Prupe.5G238400_v2.0.a1

Monoglyceride lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0010185
Physical Location & Seq
Forward (+)
18014195 .. 18021043
6849 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G238400.4

Sequence Viewer

Length: 1440 bp
ATGATCTGTTTGGGAACAACAGCGATTATAGAAACTAGAATTCTGGTGTTTCATGTGAAGAAGAGAAACAGCCATAGAAAACCAAAGCAGCTATTCATAGAAGAAGCGGAGGTCCAATCCCAATCCCAAACCCAATTCAAGTCATCGTCTTCGTCGTCAGCATCATCAGCATTACTGCTGCTTCACACCAGAAGATCAAGCCTCAAACCGAAACACCAAATGGCGGCGGAGCGAGGCAACGATGCGTCGTCGTCTTTGATCTTAACTTCTGGGGCAAGCGGCCGCATAAACGCCTTGTTCTCGCTGCGGGCGTTGCGGAGTCTGATGATGCTGATTAATGCCTTCTTCCTGCTCCTCCTGCTTCCCTTCCGCGGCCGCAAGCGAACGGCGTCGTCCTCCTCGTCTTCTTCTCCGTCGATCATTGCCTTGGCGGAGAAGTCGCCCAAGGACGAGAGGCAGCAGAGGGGGCCGGTAGTGCGGGTGCCGACGGCGATTGTGCCCTGGAAGAGCAGCTCGTCGTCGCCGGTGGACCAGGAGGTGGCGGCAAGGAGAGCTTTAGCCAAGCTTAGAGTTGTCCAAGATGATGATGAGAATTCGGTCAGGCAATTTTCCATTTTTGGAACTCCCAGAGGTGAAACCCTTTTCACGCAGAGTTGGACTCCCGTTTCGGTTAACGTCAGGGGCGTTGTCATTCTTATGCATGGCTTGAACGAACATAGTGGCAGATACAGTGACTTTGCAAAGCAGCTGAATTCTAATGGCTTCAAGGTTTATGGAATGGATTGGATTGGTCATGGTGGGAGTGAGGGGCTTCATGCATATGTTCCTTCTCTTGATTATGCTGTTACGGATATGAAATCATTTCTTGAAAAGGTTTTAGCTGAAAATCCTGGCCTACCATGTTTCTGTTTTGGACATTCCACAGGTGCAGCTATTATACTTAAGGCAATGCTTGACCCAAAAGTTGAGTCCAGCATAGAAGGTGTGGTGCTGACATCACCTGCAGTTGGGGTTCAGCCTTCGCACCCAATATTTGTGGTAATTGCTCCAATTGCCTCATTTTTGCTTCCAAGATACCAGATAAGTGCAGCAAATAAAAAGGGCATGCCCGTTTCTCGTGACCCAGCAGCTCTAGTTGCCAAGTATTCAGACCCTCTAGTGTACACTGGATCAATCCGTGTGAGGACTGGTTATGAGATTCTCCGAATCACATCCTACTTGCAGCAGAATCTAAGCAAATTGAGAGTCCCCTTCTTGGTTCTCCATGGCACCGCTGATACTGTTACTGACCCTGAAGCCTCCCAGAAACTGTACAATGAAGCCTCCTCAACTGACAAAAGCATAAAATTGTTTGACGGGTTATTACATGACCTCCTCTTTGAGCCAGAACGAGAAGCTATTGCCAAGGACATTATCGACTGGTTGAATCAGAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

480

Amino Acids

52.59

Weight (kDa)

9.61

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1009
Acc36I ACCTGC 1 cut(s) 1009
AccB1I GGYRCC 2 cut(s) 481, 1268
AccB7I CCANNNNNTGG 1 cut(s) 538
AccII CGCG 1 cut(s) 372
AclWI GGATC 1 cut(s) 1177
AcoI YGGCCR 2 cut(s) 280, 373
AcsI RAATTY 3 cut(s) 39, 592, 751
AcuI CTGAAG 1 cut(s) 1314
AcyI GRCGYC 1 cut(s) 389
AfaI GTAC 2 cut(s) 1163, 1313
AfiI CCNNNNNNNGG 5 cut(s) 223, 371, 538, 1007, 1255
AflII CTTAAG 1 cut(s) 941
AgsI TTSAA 5 cut(s) 139, 709, 766, 869, 1426
AjnI CCWGG 3 cut(s) 500, 531, 889
AluBI AGCT 9 cut(s) 91, 513, 554, 565, 748, 881, 932, 1130, 1397
AluI AGCT 9 cut(s) 91, 513, 554, 565, 748, 881, 932, 1130, 1397
AlwI GGATC 1 cut(s) 1177
AlwNI CAGNNNCTG 1 cut(s) 1309
AoxI GGCC 4 cut(s) 280, 373, 467, 892
ApoI RAATTY 3 cut(s) 39, 592, 751
ArsI GACNNNNNNTTYG 8 cut(s) 649, 681, 953, 985, 1230, 1262, 1361, 1393
AseI ATTAAT 1 cut(s) 336
AspS9I GGNCC 3 cut(s) 112, 467, 529
AsuHPI GGTGA 2 cut(s) 644, 990
AvaII GGWCC 2 cut(s) 112, 529
BaeGI GKGCMC 1 cut(s) 501
BanI GGYRCC 2 cut(s) 481, 1268
BauI CACGAG 1 cut(s) 1116
BbsI GAAGAC 2 cut(s) 141, 396
BceAI ACGGC 2 cut(s) 402, 504
BciT130I CCWGG 3 cut(s) 502, 533, 891
BfaI CTAG 3 cut(s) 36, 1133, 1157
BfmI CTRYAG 1 cut(s) 1002
BfrI CTTAAG 1 cut(s) 941
BfuAI ACCTGC 1 cut(s) 1009
Bme1390I CCNGG 3 cut(s) 502, 533, 891
Bme18I GGWCC 2 cut(s) 112, 529
BmgT120I GGNCC 3 cut(s) 112, 467, 529
BmiI GGNNCC 3 cut(s) 468, 483, 1270
BmrFI CCNGG 3 cut(s) 502, 533, 891
BmsI GCATC 3 cut(s) 170, 232, 318
BpiI GAAGAC 2 cut(s) 141, 396
BplI GAGNNNNNCTC 2 cut(s) 643, 675
BsaHI GRCGYC 1 cut(s) 389
BsaJI CCNNGG 6 cut(s) 370, 426, 444, 500, 1264, 1404
BsaXI ACNNNNNCTCC 2 cut(s) 1356, 1386
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 371, 538, 1007, 1255
Bse118I RCCGGY 2 cut(s) 469, 523
Bse1I ACTGG 3 cut(s) 1171, 1192, 1424
Bse3DI GCAATG 2 cut(s) 420, 954
BseBI CCWGG 3 cut(s) 502, 533, 891
BseDI CCNNGG 6 cut(s) 370, 426, 444, 500, 1264, 1404
BseGI GGATG 1 cut(s) 1211
BseLI CCNNNNNNNGG 5 cut(s) 223, 371, 538, 1007, 1255
BseMI GCAATG 2 cut(s) 420, 954
BseNI ACTGG 3 cut(s) 1171, 1192, 1424
BseRI GAGGAG 4 cut(s) 344, 388, 1315, 1364
BseSI GKGCMC 1 cut(s) 501
BseX3I CGGCCG 2 cut(s) 280, 373
BseYI CCCAGC 1 cut(s) 1123
BsgI GTGCAG 2 cut(s) 948, 1107
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 2 cut(s) 283, 376
BshFI GGCC 4 cut(s) 282, 375, 469, 894
BshNI GGYRCC 2 cut(s) 481, 1268
BsiEI CGRYCG 2 cut(s) 283, 376
BsiSI CCGG 2 cut(s) 470, 524
BslFI GGGAC 1 cut(s) 1232
BslI CCNNNNNNNGG 5 cut(s) 223, 371, 538, 1007, 1255
BsmFI GGGAC 1 cut(s) 1232
BsnI GGCC 4 cut(s) 282, 375, 469, 894
Bsp1286I GDGCHC 1 cut(s) 501
Bsp1407I TGTACA 2 cut(s) 1161, 1311
Bsp143I GATC 5 cut(s) 3, 194, 258, 417, 1169
Bsp19I CCATGG 1 cut(s) 1264
BspANI GGCC 4 cut(s) 282, 375, 469, 894
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 3 cut(s) 468, 483, 1270
BspMAI CTGCAG 1 cut(s) 1006
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 1 cut(s) 1177
BspQI GCTCTTC 1 cut(s) 500
BspT107I GGYRCC 2 cut(s) 481, 1268
BspTI CTTAAG 1 cut(s) 941
BsrDI GCAATG 2 cut(s) 420, 954
BsrFI RCCGGY 2 cut(s) 469, 523
BsrGI TGTACA 2 cut(s) 1161, 1311
BsrI ACTGG 3 cut(s) 1171, 1192, 1424
BssAI RCCGGY 2 cut(s) 469, 523
BssECI CCNNGG 6 cut(s) 370, 426, 444, 500, 1264, 1404
BssMI GATC 5 cut(s) 3, 194, 258, 417, 1169
BssNI GRCGYC 1 cut(s) 389
BssSI CACGAG 1 cut(s) 1116
BssT1I CCWWGG 4 cut(s) 426, 444, 1264, 1404
Bst2BI CACGAG 1 cut(s) 1116
Bst2UI CCWGG 3 cut(s) 502, 533, 891
Bst4CI ACNGT 3 cut(s) 731, 1282, 1311
Bst6I CTCTTC 2 cut(s) 56, 500
BstACI GRCGYC 1 cut(s) 389
BstAFI CTTAAG 1 cut(s) 941
BstAUI TGTACA 2 cut(s) 1161, 1311
BstC8I GCNNGC 4 cut(s) 277, 309, 380, 1106
BstDEI CTNAG 2 cut(s) 566, 1232
BstDSI CCRYGG 2 cut(s) 370, 1264
BstF5I GGATG 1 cut(s) 1211
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 5 cut(s) 6, 197, 261, 420, 1172
BstMBI GATC 5 cut(s) 3, 194, 258, 417, 1169
BstMCI CGRYCG 2 cut(s) 283, 376
BstMWI GCNNNNNNNGC 8 cut(s) 167, 313, 358, 466, 475, 551, 1052, 1136
BstNI CCWGG 3 cut(s) 502, 533, 891
BstNSI RCATGY 1 cut(s) 1108
BstSCI CCNGG 3 cut(s) 500, 531, 889
BstSFI CTRYAG 1 cut(s) 1002
BstSLI GKGCMC 1 cut(s) 501
BstUI CGCG 1 cut(s) 372
BstV2I GAAGAC 2 cut(s) 141, 396
BstZI CGGCCG 2 cut(s) 280, 373
BsuRI GGCC 4 cut(s) 282, 375, 469, 894
BtgI CCRYGG 2 cut(s) 370, 1264
BtsCI GGATG 1 cut(s) 1211
BtsIMutI CAGTG 2 cut(s) 736, 1164
BveI ACCTGC 1 cut(s) 1009
Cac8I GCNNGC 4 cut(s) 277, 309, 380, 1106
CaiI CAGNNNCTG 1 cut(s) 1309
CciNI GCGGCCGC 2 cut(s) 280, 373
Cfr10I RCCGGY 2 cut(s) 469, 523
Cfr13I GGNCC 3 cut(s) 112, 467, 529
Cfr42I CCGCGG 1 cut(s) 373
CseI GACGC 2 cut(s) 234, 378
Csp6I GTAC 2 cut(s) 1162, 1312
CspCI CAANNNNNGTGG 2 cut(s) 1017, 1052
CviAII CATG 8 cut(s) 53, 701, 794, 815, 900, 1105, 1265, 1367
CviQI GTAC 2 cut(s) 1162, 1312
DdeI CTNAG 2 cut(s) 566, 1232
DpnI GATC 5 cut(s) 5, 196, 260, 419, 1171
DpnII GATC 5 cut(s) 3, 194, 258, 417, 1169
EaeI YGGCCR 2 cut(s) 280, 373
EagI CGGCCG 2 cut(s) 280, 373
Eam1104I CTCTTC 2 cut(s) 56, 500
EarI CTCTTC 2 cut(s) 56, 500
EciI GGCGGA 2 cut(s) 242, 446
EclXI CGGCCG 2 cut(s) 280, 373
Eco130I CCWWGG 4 cut(s) 426, 444, 1264, 1404
Eco47I GGWCC 2 cut(s) 112, 529
Eco52I CGGCCG 2 cut(s) 280, 373
Eco57I CTGAAG 1 cut(s) 1314
EcoRI GAATTC 3 cut(s) 39, 592, 751
EcoRII CCWGG 3 cut(s) 500, 531, 889
EcoT14I CCWWGG 4 cut(s) 426, 444, 1264, 1404
EcoT22I ATGCAT 2 cut(s) 702, 820
ErhI CCWWGG 4 cut(s) 426, 444, 1264, 1404
FaeI CATG 8 cut(s) 56, 704, 797, 818, 903, 1108, 1268, 1370
FalI AAGNNNNNCTT 2 cut(s) 538, 570
FaqI GGGAC 1 cut(s) 1232
FatI CATG 8 cut(s) 52, 700, 793, 814, 899, 1104, 1264, 1366
FauI CCCGC 2 cut(s) 300, 471
FauNDI CATATG 1 cut(s) 820
FokI GGATG 1 cut(s) 1198
FspBI CTAG 3 cut(s) 36, 1133, 1157
GsaI CCCAGC 1 cut(s) 1127
HaeIII GGCC 4 cut(s) 282, 375, 469, 894
HapII CCGG 2 cut(s) 470, 524
HgaI GACGC 2 cut(s) 234, 378
Hin1I GRCGYC 1 cut(s) 389
Hin1II CATG 8 cut(s) 56, 704, 797, 818, 903, 1108, 1268, 1370
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 8 cut(s) 319, 658, 968, 1198, 1206, 1228, 1245, 1426
HpaI GTTAAC 1 cut(s) 673
HpaII CCGG 2 cut(s) 470, 524
HphI GGTGA 2 cut(s) 644, 990
Hpy166II GTNNAC 4 cut(s) 529, 673, 1162, 1164
Hpy188I TCNGA 4 cut(s) 324, 1150, 1205, 1431
Hpy188III TCNNGA 3 cut(s) 833, 866, 1118
Hpy8I GTNNAC 4 cut(s) 529, 673, 1162, 1164
Hpy99I CGWCG 8 cut(s) 157, 250, 253, 394, 418, 490, 520, 523
HpyAV CCTTC 6 cut(s) 352, 376, 837, 974, 1029, 1261
HpyCH4III ACNGT 3 cut(s) 731, 1282, 1311
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 7 cut(s) 700, 740, 818, 929, 1004, 1088, 1222
HpyF10VI GCNNNNNNNGC 8 cut(s) 167, 313, 358, 466, 475, 551, 1052, 1136
HpyF3I CTNAG 2 cut(s) 566, 1232
HpySE526I ACGT 1 cut(s) 675
Hsp92I GRCGYC 1 cut(s) 389
Hsp92II CATG 8 cut(s) 56, 704, 797, 818, 903, 1108, 1268, 1370
KspAI GTTAAC 1 cut(s) 673
KspI CCGCGG 1 cut(s) 373
Kzo9I GATC 5 cut(s) 3, 194, 258, 417, 1169
LguI GCTCTTC 1 cut(s) 500
LmnI GCTCC 3 cut(s) 229, 357, 1051
LweI GCATC 3 cut(s) 170, 232, 318
MaeI CTAG 3 cut(s) 36, 1133, 1157
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 4 cut(s) 731, 844, 1118, 1282
MalI GATC 5 cut(s) 5, 196, 260, 419, 1171
MboI GATC 5 cut(s) 3, 194, 258, 417, 1169
MboII GAAGA 9 cut(s) 70, 73, 113, 141, 204, 337, 396, 399, 517
MfeI CAATTG 1 cut(s) 1050
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 9 cut(s) 39, 134, 592, 605, 751, 1041, 1050, 1238, 1346
MlyI GAGTC 4 cut(s) 328, 652, 977, 1254
MmeI TCCRAC 1 cut(s) 635
Mph1103I ATGCAT 2 cut(s) 702, 820
MseI TTAA 4 cut(s) 263, 336, 672, 942
MslI CAYNNNNRTG 2 cut(s) 695, 819
MspA1I CMGCKG 3 cut(s) 372, 748, 1274
MspCI CTTAAG 1 cut(s) 941
MspI CCGG 2 cut(s) 470, 524
MspR9I CCNGG 3 cut(s) 502, 533, 891
MunI CAATTG 1 cut(s) 1050
MvaI CCWGG 3 cut(s) 502, 533, 891
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 8 cut(s) 167, 313, 358, 466, 475, 551, 1052, 1136
NcoI CCATGG 1 cut(s) 1264
NdeI CATATG 1 cut(s) 820
NdeII GATC 5 cut(s) 3, 194, 258, 417, 1169
NlaIII CATG 8 cut(s) 56, 704, 797, 818, 903, 1108, 1268, 1370
NlaIV GGNNCC 3 cut(s) 468, 483, 1270
NmuCI GTSAC 2 cut(s) 731, 1118
NotI GCGGCCGC 2 cut(s) 280, 373
NsiI ATGCAT 2 cut(s) 702, 820
NspI RCATGY 1 cut(s) 1108
PaeI GCATGC 1 cut(s) 1108
PaqCI CACCTGC 1 cut(s) 1009
PciSI GCTCTTC 1 cut(s) 500
PcsI WCGNNNNNNNCGW 4 cut(s) 152, 308, 398, 681
PfeI GAWTC 4 cut(s) 1198, 1206, 1228, 1426
PflMI CCANNNNNTGG 1 cut(s) 538
PleI GAGTC 4 cut(s) 327, 652, 976, 1253
PpsI GAGTC 4 cut(s) 327, 652, 976, 1253
PshBI ATTAAT 1 cut(s) 336
Psp6I CCWGG 3 cut(s) 500, 531, 889
PspFI CCCAGC 1 cut(s) 1123
PspGI CCWGG 3 cut(s) 500, 531, 889
PspN4I GGNNCC 3 cut(s) 468, 483, 1270
PspPI GGNCC 3 cut(s) 112, 467, 529
PstI CTGCAG 1 cut(s) 1006
PstNI CAGNNNCTG 1 cut(s) 1309
PvuII CAGCTG 1 cut(s) 748
RsaI GTAC 2 cut(s) 1163, 1313
RsaNI GTAC 2 cut(s) 1162, 1312
RseI CAYNNNNRTG 2 cut(s) 695, 819
SacII CCGCGG 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 500
SaqAI TTAA 4 cut(s) 263, 336, 672, 942
Sau3AI GATC 5 cut(s) 3, 194, 258, 417, 1169
Sau96I GGNCC 3 cut(s) 112, 467, 529
SchI GAGTC 4 cut(s) 328, 652, 977, 1254
ScrFI CCNGG 3 cut(s) 502, 533, 891
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 3 cut(s) 170, 232, 318
SfcI CTRYAG 1 cut(s) 1002
Sfr303I CCGCGG 1 cut(s) 373
SgrAI CRCCGGYG 1 cut(s) 523
SgrBI CCGCGG 1 cut(s) 373
SinI GGWCC 2 cut(s) 112, 529
SmiMI CAYNNNNRTG 2 cut(s) 695, 819
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
SphI GCATGC 1 cut(s) 1108
Sse9I AATT 9 cut(s) 39, 134, 592, 605, 751, 1041, 1050, 1238, 1346
SspI AATATT 1 cut(s) 1032
SspMI CTAG 3 cut(s) 36, 1133, 1157
StyD4I CCNGG 3 cut(s) 500, 531, 889
StyI CCWWGG 4 cut(s) 426, 444, 1264, 1404
TaaI ACNGT 3 cut(s) 731, 1282, 1311
TaiI ACGT 1 cut(s) 678
TaqI TCGA 2 cut(s) 416, 1416
TaqII GACCGA 1 cut(s) 586
TasI AATT 9 cut(s) 39, 134, 592, 605, 751, 1041, 1050, 1238, 1346
TatI WGTACW 2 cut(s) 1161, 1311
TauI GCSGC 6 cut(s) 227, 282, 285, 375, 378, 545
TfiI GAWTC 4 cut(s) 1198, 1206, 1228, 1426
Tru1I TTAA 4 cut(s) 263, 336, 672, 942
Tru9I TTAA 4 cut(s) 263, 336, 672, 942
TscAI CASTG 2 cut(s) 736, 1171
TseFI GTSAC 2 cut(s) 731, 1118
Tsp45I GTSAC 2 cut(s) 731, 1118
TspDTI ATGAA 5 cut(s) 41, 85, 803, 869, 1332
TspGWI ACGGA 3 cut(s) 402, 863, 1166
TspRI CASTG 2 cut(s) 736, 1171
Van91I CCANNNNNTGG 1 cut(s) 538
Vha464I CTTAAG 1 cut(s) 941
VpaK11BI GGWCC 2 cut(s) 112, 529
VspI ATTAAT 1 cut(s) 336
XapI RAATTY 3 cut(s) 39, 592, 751
XceI RCATGY 1 cut(s) 1108
XspI CTAG 3 cut(s) 36, 1133, 1157
Zsp2I ATGCAT 2 cut(s) 702, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.