pycom06g20840

Monoglyceride lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010185
Physical Location & Seq
Forward (+)
23744313 .. 23748517
4205 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20840.4

Sequence Viewer

Length: 1458 bp
ATGGTCTGTTTGGGAAGGGCAGCGATTATAGAGAGTAGAATTCTGGTTTTGCACGCGGAGAAGGGGCAGAAGCCAAACAACAACCTCAACAGGAAACAACCAATCCGCCCGTTGGTCGAATCGGAGGAGCTCCAATTCCGGTTCAAGTCATCATCTCCACCTCCGCCGGCGTCATCAGCATTGTTCTTTCAAGCCACAAGACCACCGCCGAAGAAGCTGCAGAAAATGGCGACGGATCGAGGCAGCGGCTTGTCATCGTCGTCGACGTTGATGTTGACTTCTGGAGCGAGCGGCCGCATAAACGCCCTCTTCTCGCTGCAGGTGCTGCGGAGTCTGATGATGCTGATTAACGCCTTCTTCCTGCTCCTCCTGCTCCCATTTCGCGGCCGCAAGCGAGCGCCGTTGTCGTCCGCCTCTTCGTCTGCGGCGGCGGCTTCGCCTGTCTTGGCGGAGAAGTCGGCCAAGGACGAGAGGCAGCCGAGGGTGCCGGTGGTGCGGGTGCCGACGAAGATAGTGCCGTGGAAGTGCAGCTCGTCATCGCCGGTGGACCAGGAGGCGGCGGCGAGGAGGGCGTTAGCGAAGCTAAGAGTTGTACAAGATGATGATGAGAATTCGGTCAGGCAATATTCGCTCTTTGCGACTGCGCGAGGTGAAACCCTCTTCACGCAGAGTTGGACTCCCGTTTCCGTTAACATCAGGGGCGTAGTTATTCTTATGCATGGTCTGAACGAGCACAGTGGAAGATACAGCGACTTTGCAAGGCAACTGAATGCTAATGGATTTAAGGTTTACGGAATGGATTGGATTGGTCATGGCGGGAGTGATGGGCTTCATGCATTCGTACCTTCTCTCGATTATGCTGTTACTGATATGAAATTATTTCTCGAAAAGGTTTTAGCTGAAAATCATGGCCTACCGTGTTTCTGTTTTGGGCATTCCACAGGTGCAGCCATTGTCCTTAAGGTAATGCTTGACCCAAAATTTGAGTCCCGCATAGACGGTGTAGTGCTCACATCACCTGCAGTTGGGGTTCAGCCTTCGCATCCAATATTAGTGGTAATTGCTCCCATTGTCTCATTTTTGCTGCCAAGATACCAAATCAGTGCAGCAAATAAACAGGGCATGCCAGTTTCTCGTGACCCGGCAGCTTTAGTTGCCAAGTATTCGGACCCTCTAGTGTACACTGGATCCATCCGCGTGAGGACTGGTTATGAGATTCTCCGAATCACATCCTACTTGCAGCAGAACCTAACCAAATTGATGGTACCCTTCTTCGTTCTCCACGGAACAGCGGATACTGTTACTGACCCCGAGGCCTCCCAGAAGCTGTACGATGAAGCCTCCTCAACTGACAAAACCATCAAGCTGTTTGACGGGTTCGTACATGACCTTCTCTTTGAGCCAGAGCGAGAAGCCATTACGAAGGATATCATTGACTGGTTGAATCATAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

486

Amino Acids

53.33

Weight (kDa)

9.53

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 310, 1027
Acc36I ACCTGC 2 cut(s) 310, 1027
Acc65I GGTACC 1 cut(s) 1264
AccB1I GGYRCC 3 cut(s) 484, 499, 1264
AccBSI CCGCTC 1 cut(s) 291
AccI GTMKAC 1 cut(s) 263
AccII CGCG 4 cut(s) 56, 384, 646, 1197
AclWI GGATC 3 cut(s) 243, 1182, 1195
AcoI YGGCCR 3 cut(s) 292, 385, 459
AcsI RAATTY 3 cut(s) 39, 610, 980
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 6 cut(s) 594, 843, 1181, 1266, 1331, 1383
AfiI CCNNNNNNNGG 4 cut(s) 112, 383, 556, 1025
AflII CTTAAG 1 cut(s) 959
AgsI TTSAA 3 cut(s) 145, 191, 1444
AjnI CCWGG 1 cut(s) 549
AluBI AGCT 8 cut(s) 130, 217, 531, 583, 899, 1148, 1327, 1366
AluI AGCT 8 cut(s) 130, 217, 531, 583, 899, 1148, 1327, 1366
Alw21I GWGCWC 3 cut(s) 132, 735, 1011
Alw26I GTCTC 1 cut(s) 1078
AlwI GGATC 3 cut(s) 243, 1182, 1195
AlwNI CAGNNNCTG 2 cut(s) 325, 1327
Ama87I CYCGRG 1 cut(s) 1310
AoxI GGCC 5 cut(s) 292, 385, 459, 910, 1314
ApoI RAATTY 3 cut(s) 39, 610, 980
ArsI GACNNNNNNTTYG 6 cut(s) 965, 971, 997, 1003, 1379, 1411
Asp718I GGTACC 1 cut(s) 1264
AspLEI GCGC 2 cut(s) 400, 646
AspS9I GGNCC 2 cut(s) 547, 1168
AsuC2I CCSGG 1 cut(s) 1142
AsuHPI GGTGA 2 cut(s) 662, 1008
AvaI CYCGRG 1 cut(s) 1310
AvaII GGWCC 2 cut(s) 547, 1168
BamHI GGATCC 1 cut(s) 1187
BanI GGYRCC 3 cut(s) 484, 499, 1264
BanII GRGCYC 1 cut(s) 132
BarI GAAGNNNNNNTAC 2 cut(s) 1374, 1406
BauI CACGAG 1 cut(s) 1134
Bbv12I GWGCWC 3 cut(s) 132, 735, 1011
BccI CCATC 4 cut(s) 818, 1199, 1255, 1367
BceAI ACGGC 2 cut(s) 385, 502
BcgI CGANNNNNNTGC 4 cut(s) 199, 233, 496, 530
BciT130I CCWGG 1 cut(s) 551
BciVI GTATCC 1 cut(s) 1288
BcnI CCSGG 1 cut(s) 1142
BcoDI GTCTC 1 cut(s) 1078
BfaI CTAG 1 cut(s) 1175
BfmI CTRYAG 3 cut(s) 218, 317, 1020
BfoI RGCGCY 1 cut(s) 401
BfrI CTTAAG 1 cut(s) 959
BfuAI ACCTGC 2 cut(s) 310, 1027
BfuI GTATCC 1 cut(s) 1288
Bme1390I CCNGG 2 cut(s) 551, 1142
Bme18I GGWCC 2 cut(s) 547, 1168
BmeT110I CYCGRG 1 cut(s) 1310
BmgT120I GGNCC 2 cut(s) 547, 1168
BmiI GGNNCC 5 cut(s) 486, 501, 1170, 1189, 1266
BmrFI CCNGG 2 cut(s) 551, 1142
BmsI GCATC 2 cut(s) 330, 1051
BplI GAGNNNNNCTC 2 cut(s) 661, 693
BpmI CTGGAG 1 cut(s) 303
BpuMI CCSGG 1 cut(s) 1142
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 5 cut(s) 462, 479, 518, 1282, 1311
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 383, 556, 1025
Bse118I RCCGGY 3 cut(s) 166, 487, 541
Bse1I ACTGG 4 cut(s) 1127, 1189, 1210, 1442
BseBI CCWGG 1 cut(s) 551
BseDI CCNNGG 5 cut(s) 462, 479, 518, 1282, 1311
BseGI GGATG 3 cut(s) 1042, 1191, 1229
BseLI CCNNNNNNNGG 4 cut(s) 112, 383, 556, 1025
BseNI ACTGG 4 cut(s) 1127, 1189, 1210, 1442
BseRI GAGGAG 4 cut(s) 140, 356, 580, 1333
BseX3I CGGCCG 2 cut(s) 292, 385
BsgI GTGCAG 3 cut(s) 547, 966, 1125
Bsh1236I CGCG 4 cut(s) 56, 384, 646, 1197
Bsh1285I CGRYCG 2 cut(s) 295, 388
BshFI GGCC 5 cut(s) 294, 387, 461, 912, 1316
BshNI GGYRCC 3 cut(s) 484, 499, 1264
BsiEI CGRYCG 2 cut(s) 295, 388
BsiHKAI GWGCWC 3 cut(s) 132, 735, 1011
BsiHKCI CYCGRG 1 cut(s) 1310
BsiSI CCGG 5 cut(s) 139, 167, 488, 542, 1142
BslFI GGGAC 1 cut(s) 973
BslI CCNNNNNNNGG 4 cut(s) 112, 383, 556, 1025
BsmAI GTCTC 1 cut(s) 1078
BsmFI GGGAC 1 cut(s) 973
BsmI GAATGC 3 cut(s) 775, 836, 934
BsnI GGCC 5 cut(s) 294, 387, 461, 912, 1316
BsoBI CYCGRG 1 cut(s) 1310
Bsp1286I GDGCHC 3 cut(s) 132, 735, 1011
Bsp1407I TGTACA 2 cut(s) 592, 1179
Bsp143I GATC 2 cut(s) 235, 1187
BspANI GGCC 5 cut(s) 294, 387, 461, 912, 1316
BspFNI CGCG 4 cut(s) 56, 384, 646, 1197
BspLI GGNNCC 5 cut(s) 486, 501, 1170, 1189, 1266
BspMAI CTGCAG 3 cut(s) 222, 321, 1024
BspMI ACCTGC 2 cut(s) 310, 1027
BspPI GGATC 3 cut(s) 243, 1182, 1195
BspT107I GGYRCC 3 cut(s) 484, 499, 1264
BspTI CTTAAG 1 cut(s) 959
BsrBI CCGCTC 1 cut(s) 291
BsrFI RCCGGY 3 cut(s) 166, 487, 541
BsrGI TGTACA 2 cut(s) 592, 1179
BsrI ACTGG 4 cut(s) 1127, 1189, 1210, 1442
BssAI RCCGGY 3 cut(s) 166, 487, 541
BssECI CCNNGG 5 cut(s) 462, 479, 518, 1282, 1311
BssMI GATC 2 cut(s) 235, 1187
BssNI GRCGYC 1 cut(s) 170
BssSI CACGAG 1 cut(s) 1134
BssT1I CCWWGG 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 551
Bst4CI ACNGT 4 cut(s) 737, 918, 1001, 1300
Bst6I CTCTTC 3 cut(s) 314, 421, 665
BstACI GRCGYC 1 cut(s) 170
BstAFI CTTAAG 1 cut(s) 959
BstAPI GCANNNNNTGC 1 cut(s) 325
BstAUI TGTACA 2 cut(s) 592, 1179
BstC8I GCNNGC 6 cut(s) 54, 168, 289, 392, 396, 1124
BstDEI CTNAG 1 cut(s) 584
BstDSI CCRYGG 2 cut(s) 518, 1282
BstF5I GGATG 3 cut(s) 1042, 1191, 1229
BstFNI CGCG 4 cut(s) 56, 384, 646, 1197
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 400, 646
BstKTI GATC 2 cut(s) 238, 1190
BstMAI GTCTC 1 cut(s) 1078
BstMBI GATC 2 cut(s) 235, 1187
BstMCI CGRYCG 2 cut(s) 295, 388
BstNI CCWGG 1 cut(s) 551
BstNSI RCATGY 1 cut(s) 1126
BstSCI CCNGG 2 cut(s) 549, 1140
BstSFI CTRYAG 3 cut(s) 218, 317, 1020
BstUI CGCG 4 cut(s) 56, 384, 646, 1197
BstX2I RGATCY 1 cut(s) 1187
BstXI CCANNNNNNTGG 1 cut(s) 1261
BstYI RGATCY 1 cut(s) 1187
BstZI CGGCCG 2 cut(s) 292, 385
BsuI GTATCC 1 cut(s) 1288
BsuRI GGCC 5 cut(s) 294, 387, 461, 912, 1316
BtgI CCRYGG 2 cut(s) 518, 1282
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 3 cut(s) 1042, 1191, 1229
BtsIMutI CAGTG 3 cut(s) 742, 1108, 1182
BveI ACCTGC 2 cut(s) 310, 1027
Cac8I GCNNGC 6 cut(s) 54, 168, 289, 392, 396, 1124
CaiI CAGNNNCTG 2 cut(s) 325, 1327
CciNI GCGGCCGC 2 cut(s) 292, 385
CfoI GCGC 2 cut(s) 400, 646
Cfr10I RCCGGY 3 cut(s) 166, 487, 541
Cfr13I GGNCC 2 cut(s) 547, 1168
CseI GACGC 1 cut(s) 159
Csp6I GTAC 6 cut(s) 593, 842, 1180, 1265, 1330, 1382
CspCI CAANNNNNGTGG 2 cut(s) 1035, 1070
CviAII CATG 6 cut(s) 719, 812, 833, 908, 1123, 1385
CviQI GTAC 6 cut(s) 593, 842, 1180, 1265, 1330, 1382
DdeI CTNAG 1 cut(s) 584
DpnI GATC 2 cut(s) 237, 1189
DpnII GATC 2 cut(s) 235, 1187
EaeI YGGCCR 3 cut(s) 292, 385, 459
EagI CGGCCG 2 cut(s) 292, 385
Eam1104I CTCTTC 3 cut(s) 314, 421, 665
EarI CTCTTC 3 cut(s) 314, 421, 665
EciI GGCGGA 4 cut(s) 95, 153, 400, 464
Ecl136II GAGCTC 1 cut(s) 130
EclXI CGGCCG 2 cut(s) 292, 385
Eco130I CCWWGG 1 cut(s) 462
Eco147I AGGCCT 1 cut(s) 1316
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 1429
Eco47I GGWCC 2 cut(s) 547, 1168
Eco52I CGGCCG 2 cut(s) 292, 385
Eco53kI GAGCTC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 1310
EcoICRI GAGCTC 1 cut(s) 130
EcoRI GAATTC 2 cut(s) 39, 610
EcoRII CCWGG 1 cut(s) 549
EcoRV GATATC 1 cut(s) 1429
EcoT14I CCWWGG 1 cut(s) 462
EcoT22I ATGCAT 2 cut(s) 720, 838
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 6 cut(s) 722, 815, 836, 911, 1126, 1388
FaqI GGGAC 1 cut(s) 973
FatI CATG 6 cut(s) 718, 811, 832, 907, 1122, 1384
FauI CCCGC 3 cut(s) 489, 809, 998
FblI GTMKAC 1 cut(s) 263
FokI GGATG 3 cut(s) 1029, 1178, 1216
FriOI GRGCYC 1 cut(s) 132
FspBI CTAG 1 cut(s) 1175
GlaI GCGC 2 cut(s) 399, 645
GsuI CTGGAG 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 5 cut(s) 294, 387, 461, 912, 1316
HapII CCGG 5 cut(s) 139, 167, 488, 542, 1142
HgaI GACGC 1 cut(s) 159
HhaI GCGC 2 cut(s) 400, 646
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 6 cut(s) 722, 815, 836, 911, 1126, 1388
Hin6I GCGC 2 cut(s) 398, 644
HinP1I GCGC 2 cut(s) 398, 644
HincII GTYRAC 3 cut(s) 264, 276, 691
HindII GTYRAC 3 cut(s) 264, 276, 691
HinfI GANTC 7 cut(s) 119, 331, 676, 986, 1216, 1224, 1444
HpaI GTTAAC 1 cut(s) 691
HpaII CCGG 5 cut(s) 139, 167, 488, 542, 1142
HphI GGTGA 2 cut(s) 662, 1008
Hpy166II GTNNAC 7 cut(s) 264, 276, 547, 691, 790, 1180, 1182
Hpy188I TCNGA 5 cut(s) 124, 336, 726, 1168, 1223
Hpy188III TCNNGA 4 cut(s) 282, 851, 884, 1136
Hpy8I GTNNAC 7 cut(s) 264, 276, 547, 691, 790, 1180, 1182
Hpy99I CGWCG 5 cut(s) 235, 262, 265, 268, 508
HpyAV CCTTC 8 cut(s) 9, 55, 364, 855, 1047, 1279, 1400, 1417
HpyCH4III ACNGT 4 cut(s) 737, 918, 1001, 1300
HpyCH4IV ACGT 1 cut(s) 266
HpyF3I CTNAG 1 cut(s) 584
HpySE526I ACGT 1 cut(s) 266
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 6 cut(s) 722, 815, 836, 911, 1126, 1388
HspAI GCGC 2 cut(s) 398, 644
KpnI GGTACC 1 cut(s) 1268
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
KspAI GTTAAC 1 cut(s) 691
Kzo9I GATC 2 cut(s) 235, 1187
LmnI GCTCC 6 cut(s) 127, 135, 284, 369, 378, 1069
LweI GCATC 2 cut(s) 330, 1051
MaeI CTAG 1 cut(s) 1175
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 3 cut(s) 862, 1136, 1300
MalI GATC 2 cut(s) 237, 1189
MbiI CCGCTC 1 cut(s) 291
MboI GATC 2 cut(s) 235, 1187
MboII GAAGA 8 cut(s) 223, 301, 349, 408, 520, 652, 753, 1264
MflI RGATCY 1 cut(s) 1187
MhlI GDGCHC 3 cut(s) 132, 735, 1011
MluCI AATT 7 cut(s) 39, 134, 610, 875, 980, 1059, 1256
MlyI GAGTC 3 cut(s) 340, 670, 995
MmeI TCCRAC 1 cut(s) 653
Mph1103I ATGCAT 2 cut(s) 720, 838
MreI CGCCGGCG 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 166
MseI TTAA 4 cut(s) 348, 690, 783, 960
MslI CAYNNNNRTG 1 cut(s) 1196
MspA1I CMGCKG 2 cut(s) 246, 1292
MspCI CTTAAG 1 cut(s) 959
MspI CCGG 5 cut(s) 139, 167, 488, 542, 1142
MspR9I CCNGG 2 cut(s) 551, 1142
Mva1269I GAATGC 3 cut(s) 775, 836, 934
MvaI CCWGG 1 cut(s) 551
MvnI CGCG 4 cut(s) 56, 384, 646, 1197
NaeI GCCGGC 1 cut(s) 168
NciI CCSGG 1 cut(s) 1142
NdeII GATC 2 cut(s) 235, 1187
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 6 cut(s) 722, 815, 836, 911, 1126, 1388
NlaIV GGNNCC 5 cut(s) 486, 501, 1170, 1189, 1266
NmeAIII GCCGAG 1 cut(s) 504
NmuCI GTSAC 1 cut(s) 1136
NotI GCGGCCGC 2 cut(s) 292, 385
NsiI ATGCAT 2 cut(s) 720, 838
NspI RCATGY 1 cut(s) 1126
PaeI GCATGC 1 cut(s) 1126
PaqCI CACCTGC 2 cut(s) 310, 1027
PceI AGGCCT 1 cut(s) 1316
PcsI WCGNNNNNNNCGW 2 cut(s) 263, 635
PctI GAATGC 3 cut(s) 775, 836, 934
PdiI GCCGGC 1 cut(s) 168
PfeI GAWTC 4 cut(s) 119, 1216, 1224, 1444
PleI GAGTC 3 cut(s) 339, 670, 994
PpsI GAGTC 3 cut(s) 339, 670, 994
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 549
PspGI CCWGG 1 cut(s) 549
PspN4I GGNNCC 5 cut(s) 486, 501, 1170, 1189, 1266
PspPI GGNCC 2 cut(s) 547, 1168
PsrI GAACNNNNNNTAC 2 cut(s) 1279, 1311
PstI CTGCAG 3 cut(s) 222, 321, 1024
PstNI CAGNNNCTG 2 cut(s) 325, 1327
PsuI RGATCY 1 cut(s) 1187
RsaI GTAC 6 cut(s) 594, 843, 1181, 1266, 1331, 1383
RsaNI GTAC 6 cut(s) 593, 842, 1180, 1265, 1330, 1382
RseI CAYNNNNRTG 1 cut(s) 1196
SacI GAGCTC 1 cut(s) 132
SalI GTCGAC 1 cut(s) 262
SaqAI TTAA 4 cut(s) 348, 690, 783, 960
Sau3AI GATC 2 cut(s) 235, 1187
Sau96I GGNCC 2 cut(s) 547, 1168
SchI GAGTC 3 cut(s) 340, 670, 995
ScrFI CCNGG 2 cut(s) 551, 1142
SduI GDGCHC 3 cut(s) 132, 735, 1011
SfaNI GCATC 2 cut(s) 330, 1051
SfcI CTRYAG 3 cut(s) 218, 317, 1020
SgrAI CRCCGGYG 2 cut(s) 166, 541
SgrDI CGTCGACG 1 cut(s) 262
SinI GGWCC 2 cut(s) 547, 1168
SmiMI CAYNNNNRTG 1 cut(s) 1196
SmlI CTYRAG 1 cut(s) 959
SmoI CTYRAG 1 cut(s) 959
SphI GCATGC 1 cut(s) 1126
Sse9I AATT 7 cut(s) 39, 134, 610, 875, 980, 1059, 1256
SseBI AGGCCT 1 cut(s) 1316
SspI AATATT 2 cut(s) 626, 1050
SspMI CTAG 1 cut(s) 1175
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 1 cut(s) 1316
StyD4I CCNGG 2 cut(s) 549, 1140
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 4 cut(s) 737, 918, 1001, 1300
TaiI ACGT 1 cut(s) 269
TaqI TCGA 5 cut(s) 117, 238, 263, 852, 885
TaqII GACCGA 1 cut(s) 604
TasI AATT 7 cut(s) 39, 134, 610, 875, 980, 1059, 1256
TatI WGTACW 2 cut(s) 592, 1179
TfiI GAWTC 4 cut(s) 119, 1216, 1224, 1444
Tru1I TTAA 4 cut(s) 348, 690, 783, 960
Tru9I TTAA 4 cut(s) 348, 690, 783, 960
TscAI CASTG 3 cut(s) 742, 1108, 1189
TseFI GTSAC 1 cut(s) 1136
Tsp45I GTSAC 1 cut(s) 1136
TspDTI ATGAA 3 cut(s) 821, 887, 1350
TspGWI ACGGA 4 cut(s) 248, 676, 807, 1299
TspRI CASTG 3 cut(s) 742, 1108, 1189
Vha464I CTTAAG 1 cut(s) 959
VpaK11BI GGWCC 2 cut(s) 547, 1168
XapI RAATTY 3 cut(s) 39, 610, 980
XceI RCATGY 1 cut(s) 1126
XmiI GTMKAC 1 cut(s) 263
XspI CTAG 1 cut(s) 1175
Zsp2I ATGCAT 2 cut(s) 720, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.