Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005948

Physical Location & Seq

Genomic Coordinates Reverse (-)

27943243 .. 27947886

Gene Structure 4644 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G74330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGGGTTGCGTTAGCTCGAAGCAGACGGTCTCTGTGACGCCTGCGATCGATCACTCAGGCGTCTTCAAGGATAATGAAAATGAGTGTTCTGGATCGGGTCGGATTGTGGTGGAGGATCCGCCTCGGCCGACGTTGAAGAAGCTCGTGTCGTGGAGGAGTAGGAGCGGGAAGAGGAGGAGTCAGAAGAGCGGTAGCGAGTTAGGGAGTGAGTCAGGACGTGCTAGTGACTCGCTTAGTTTTCGACTCGGTAATGTTAGTAGATACCTTGAAGCTGAGCAAGTCGCTGCTGGTTGGCCTGCGTGGTTGAGCAACGTTGCTGGTGAAGCTATCCATGGTTGGGTCCCACTTCGATCCGATGCGTTCGAAAAACTCGAAAAGATTGGACAAGGTACTTATAGCAATGTGTTTCGTGCGGTGGAGACTGAAACTGGGAGGATTGTGGCATTGAAGAAGGTGAGGTTTGATAATTTTGAGCCGGAGAGTGTTAAGTTTATGGCAAGAGAGATTTTGATACTTAGAAGACTTAATCATCCCAACATTATCAAACTGGAAGGTTTGATTACTTCAAAACTATCCTGCAACATTCAACTTGTGTTCGAGTATATGGAGCATGATCTTACTGGTCTTCTTTCTTCCCCTGACATCAAATTTACGACGCCACAGATTAAGTGTTACATGAAGCAATTGTTGTCTGGGCTTGATCATTGTCATTCACGAGGCGTGATGCATCGAGACATAAAGGGTTCGAATCTTTTGTTGAGTAATGAAGGGATATTAAAGGTGGCTGATTTTGGGTTAGCAAACTTCAGTAATAGTTCTGGACACAAGAAGAAACCTCTTACCAGTCGAGTTGTGACTCTGTGGTATCGTCCACCAGAACTTTTGCTCGGGGCAACGGACTATGGAGCATCTGTTGATTTGTGGAGTGTTGGTTGTGTTTTCGCTGAGCTGCTTCTTGGAAAACCTATTCTCCGGGGAAGAACTGAGGTTGAACAGTTGCACAAGATATTCAAACTGTGTGGATCTCCACCAGAAGATTACTGGAAAAAGTCCAAACTTCCCCATGCAATGCTTTTCAAGCCACAGCAAACTTATGATAGTTGTCTCCGCGAAACCTTGAAAGATTTGTCTGAGACTGAAATCAATCTGATAGAAACTCTTCTTTCTATAGATCCCCACAAACGCGGTACTGCCTCTAGTGCCCTTGTTTCTCAGTATTTTACTACAAAGCCTTTTGCTTGTGATCCCTCAAGCTTGCCAATATACCCACCTAGCAAGGAGATAGATACAAAGCATCGGGACGAAGCAGCAAGGAAAAAAATTAGCGGGAATGGGAGACGTGGTATAGATCCGAGGAAGCCATCAAGGAAGGCCCATTCATTCAATAGATTGGCACCAGATGTGCGGCACCAAACTGAAACATTTCAGAAACGAATTGGTCATTTAGTTCATAGTTCGATTGAAAGTGATGCCAGGTTATGTGGGAAACTGCAAAACCCGTTAGATCACAAGAAAGACGAAGCTTCCCATGTGAAGCATGCATCACAAGGAGATGTGCCTTTCTCAGGGCCGTTACAAGTCTCTAAATCAAACAGTTTTGCTTGGGCGAAACGAGAAAAAGATGACGTATGTGTTAGGGTGCATAATCGATCTCTCTCAAGAGGTTACATTCCTAGCTTGTCAGGACATTCCCCCGCATTCAATGGGAAAAGTGATGTTGAATCCAAGATAAATAAGGATGAAAAGGAGGACAAGACAGATTCTCGAGGCGAGGAGTCATATGAGATGGTGAAGCGTTCTATGCTAAAGCAGTGGAGACAACTTGAACGTCCAGATTCTTTTGGTGCATCTGACGAGTATCACTCGCAGGAACTGTCATTGGGACTCTATCAGAGAGATGAAATGGCAAAAAAGATGGGTAATAATTTGGGTGATGGGGACAAGATCGAATTCTCGGGCCCTTTGTTGTCTCAATCTTATGGAGTTGATGAACTGTTGGAACGCCATGAACGCAACATCCGCAAGCTAATTCGAAAACCGTGGTTTCAAAAAGTCCAAGACAAACAAAAAAAGATTTTGCTTTTCTCATCTGAATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009966 GO:0009987 GO:0010646 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0031347 GO:0031349 GO:0032101 GO:0032103 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0097472 GO:0098542 GO:0140096 GO:1900424 GO:1900426 GO:1901564 GO:1902288 GO:1902290 GO:2000031 GO:2001023 GO:2001038

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

699

Amino Acids

78.73

Weight (kDa)

9.26

Isoelectric Point (pI)

48.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	121 - 407	4e-67	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	123 - 333	4.9e-31	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005948)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18670 AT1G18670 AT1G18670 AT1G74330 AT1G74330 AT1G74330
fragaria_vesca	FvH4_5g14470 FvH4_5g14470 FvH4_5g14470 FvH4_5g14470 FvH4_5g14470 FvH4_5g14470
malus_domestica	MD06G1198100.v1.1 MD14G1205700.v1.1
prunus_persica	Prupe.5G199000_v2.0.a1 Prupe.5G199000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0182181
rosa_laevigata	RLG00000005143
rosa_multiflora	Rmu_sc0000003.1_g000017
rosa_roxburghii	Rroxscaffold_3G00271440
rosa_rugosa	Rorug06G0445900 Rorug06G0445900
rosa_samantha	Rh7AG049000 Rh7BG048900 Rh7CG050300 Rh7DG048200
rosa_wichuraiana	Rw7G004000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1393, 1407
AccBSI	CCGCTC	2 cut(s)	165, 189
AccII	CGCG	2 cut(s)	1110, 1185
AclI	AACGTT	1 cut(s)	312
AclWI	GGATC	8 cut(s)	100, 110, 123, 345, 1030, 1166, 1238, 1343
AcoI	YGGCCR	1 cut(s)	125
AcsI	RAATTY	2 cut(s)	647, 1949
AcuI	CTGAAG	1 cut(s)	790
AcyI	GRCGYC	3 cut(s)	38, 60, 656
AfaI	GTAC	2 cut(s)	391, 1189
AfiI	CCNNNNNNNGG	2 cut(s)	337, 1565
AjiI	CACGTC	2 cut(s)	218, 1340
AjnI	CCWGG	1 cut(s)	1472
AloI	GAACNNNNNNTCC	2 cut(s)	2001, 2033
AluBI	AGCT	9 cut(s)	15, 142, 272, 326, 949, 1254, 1523, 1677, 2026
AluI	AGCT	9 cut(s)	15, 142, 272, 326, 949, 1254, 1523, 1677, 2026
Alw26I	GTCTC	9 cut(s)	34, 413, 726, 1109, 1127, 1330, 1585, 1810, 1974
AlwI	GGATC	8 cut(s)	100, 110, 123, 345, 1030, 1166, 1238, 1343
AlwNI	CAGNNNCTG	1 cut(s)	1873
Ama87I	CYCGRG	3 cut(s)	887, 1764, 1954
AoxI	GGCC	5 cut(s)	125, 293, 1371, 1568, 1957
ApaI	GGGCCC	1 cut(s)	1961
ApeKI	GCWGC	3 cut(s)	284, 949, 1307
ApoI	RAATTY	2 cut(s)	647, 1949
Asp700I	GAANNNNTTC	2 cut(s)	1158, 1422
AspS9I	GGNCC	5 cut(s)	340, 1372, 1568, 1957, 1958
AsuC2I	CCSGG	1 cut(s)	974
AsuHPI	GGTGA	4 cut(s)	332, 466, 1801, 1943
AsuII	TTCGAA	3 cut(s)	363, 746, 2032
AvaI	CYCGRG	3 cut(s)	887, 1764, 1954
AvaII	GGWCC	1 cut(s)	340
BaeGI	GKGCMC	2 cut(s)	1204, 1961
BamHI	GGATCC	1 cut(s)	115
BanI	GGYRCC	2 cut(s)	1393, 1407
BanII	GRGCYC	1 cut(s)	1961
BauI	CACGAG	2 cut(s)	143, 714
BbsI	GAAGAC	3 cut(s)	55, 526, 617
BbvI	GCAGC	3 cut(s)	271, 936, 1319
BccI	CCATC	4 cut(s)	1369, 1780, 1909, 1928
BceAI	ACGGC	1 cut(s)	1555
BciT130I	CCWGG	1 cut(s)	1474
BclI	TGATCA	1 cut(s)	700
BcnI	CCSGG	1 cut(s)	974
BcoDI	GTCTC	9 cut(s)	34, 413, 726, 1109, 1127, 1330, 1585, 1810, 1974
BfaI	CTAG	4 cut(s)	222, 1197, 1272, 1674
BfmI	CTRYAG	1 cut(s)	1167
BisI	GCNGC	4 cut(s)	285, 950, 1308, 1406
BlpI	GCTNAGC	2 cut(s)	273, 945
BlsI	GCNGC	4 cut(s)	286, 951, 1309, 1407
Bme1390I	CCNGG	2 cut(s)	974, 1474
Bme18I	GGWCC	1 cut(s)	340
BmeT110I	CYCGRG	3 cut(s)	887, 1764, 1954
BmgBI	CACGTC	2 cut(s)	218, 1340
BmgT120I	GGNCC	5 cut(s)	340, 1372, 1568, 1957, 1958
BmiI	GGNNCC	6 cut(s)	117, 341, 342, 1395, 1409, 1959
BmrFI	CCNGG	2 cut(s)	974, 1474
BmrI	ACTGGG	1 cut(s)	438
BmsI	GCATC	8 cut(s)	346, 714, 736, 917, 1303, 1459, 1550, 1856
BmuI	ACTGGG	1 cut(s)	438
BpiI	GAAGAC	3 cut(s)	55, 526, 617
BplI	GAGNNNNNCTC	2 cut(s)	1847, 1879
Bpu1102I	GCTNAGC	2 cut(s)	273, 945
Bpu14I	TTCGAA	3 cut(s)	363, 746, 2032
BpuEI	CTTGAG	2 cut(s)	1234, 1642
BpuMI	CCSGG	1 cut(s)	974
Bsa29I	ATCGAT	2 cut(s)	48, 1648
BsaHI	GRCGYC	3 cut(s)	38, 60, 656
BsaI	GGTCTC	1 cut(s)	34
BsaJI	CCNNGG	5 cut(s)	122, 331, 973, 1352, 2039
BsaXI	ACNNNNNCTCC	8 cut(s)	897, 916, 927, 946, 954, 984, 1327, 1357
Bsc4I	CCNNNNNNNGG	2 cut(s)	337, 1565
Bse1I	ACTGG	5 cut(s)	433, 552, 625, 843, 1046
Bse3DI	GCAATG	2 cut(s)	406, 1074
BseBI	CCWGG	1 cut(s)	1474
BseCI	ATCGAT	2 cut(s)	48, 1648
BseDI	CCNNGG	5 cut(s)	122, 331, 973, 1352, 2039
BseGI	GGATG	3 cut(s)	529, 1744, 2016
BseLI	CCNNNNNNNGG	2 cut(s)	337, 1565
BseMI	GCAATG	2 cut(s)	406, 1074
BseMII	CTCAG	7 cut(s)	69, 264, 936, 975, 1122, 1226, 1578
BseNI	ACTGG	5 cut(s)	433, 552, 625, 843, 1046
BseRI	GAGGAG	4 cut(s)	169, 187, 190, 1787
BseSI	GKGCMC	2 cut(s)	1204, 1961
BseX3I	CGGCCG	1 cut(s)	125
BseXI	GCAGC	3 cut(s)	271, 936, 1319
Bsh1236I	CGCG	2 cut(s)	1110, 1185
Bsh1285I	CGRYCG	2 cut(s)	48, 128
BshFI	GGCC	5 cut(s)	127, 295, 1373, 1570, 1959
BshNI	GGYRCC	2 cut(s)	1393, 1407
BshVI	ATCGAT	2 cut(s)	48, 1648
BsiEI	CGRYCG	2 cut(s)	48, 128
BsiHKCI	CYCGRG	3 cut(s)	887, 1764, 1954
BsiSI	CCGG	2 cut(s)	476, 973
BslFI	GGGAC	4 cut(s)	326, 1313, 1896, 1952
BslI	CCNNNNNNNGG	2 cut(s)	337, 1565
BsmAI	GTCTC	9 cut(s)	34, 413, 726, 1109, 1127, 1330, 1585, 1810, 1974
BsmBI	CGTCTC	1 cut(s)	1330
BsmFI	GGGAC	4 cut(s)	326, 1313, 1896, 1952
BsmI	GAATGC	1 cut(s)	1697
BsnI	GGCC	5 cut(s)	127, 295, 1373, 1570, 1959
Bso31I	GGTCTC	1 cut(s)	34
BsoBI	CYCGRG	3 cut(s)	887, 1764, 1954
Bsp119I	TTCGAA	3 cut(s)	363, 746, 2032
Bsp120I	GGGCCC	1 cut(s)	1957
Bsp1286I	GDGCHC	2 cut(s)	1204, 1961
Bsp1720I	GCTNAGC	2 cut(s)	273, 945
Bsp19I	CCATGG	1 cut(s)	331
BspANI	GGCC	5 cut(s)	127, 295, 1373, 1570, 1959
BspCNI	CTCAG	7 cut(s)	68, 265, 937, 976, 1123, 1225, 1577
BspDI	ATCGAT	2 cut(s)	48, 1648
BspFNI	CGCG	2 cut(s)	1110, 1185
BspLI	GGNNCC	6 cut(s)	117, 341, 342, 1395, 1409, 1959
BspPI	GGATC	8 cut(s)	100, 110, 123, 345, 1030, 1166, 1238, 1343
BspQI	GCTCTTC	1 cut(s)	179
BspT104I	TTCGAA	3 cut(s)	363, 746, 2032
BspT107I	GGYRCC	2 cut(s)	1393, 1407
BspTNI	GGTCTC	1 cut(s)	34
BsrBI	CCGCTC	2 cut(s)	165, 189
BsrDI	GCAATG	2 cut(s)	406, 1074
BsrI	ACTGG	5 cut(s)	433, 552, 625, 843, 1046
BssECI	CCNNGG	5 cut(s)	122, 331, 973, 1352, 2039
BssNI	GRCGYC	3 cut(s)	38, 60, 656
BssSI	CACGAG	2 cut(s)	143, 714
BssT1I	CCWWGG	1 cut(s)	331
Bst2BI	CACGAG	2 cut(s)	143, 714
Bst2UI	CCWGG	1 cut(s)	1474
Bst4CI	ACNGT	7 cut(s)	28, 996, 1017, 1595, 1875, 1995, 2040
Bst6I	CTCTTC	3 cut(s)	164, 179, 1164
BstACI	GRCGYC	3 cut(s)	38, 60, 656
BstBI	TTCGAA	3 cut(s)	363, 746, 2032
BstC8I	GCNNGC	5 cut(s)	42, 297, 1256, 1539, 2024
BstDEI	CTNAG	9 cut(s)	55, 233, 273, 515, 945, 984, 1131, 1212, 1564
BstDSI	CCRYGG	2 cut(s)	331, 2039
BstENI	CCTNNNNNAGG	1 cut(s)	1563
BstF5I	GGATG	3 cut(s)	529, 1744, 2016
BstFNI	CGCG	2 cut(s)	1110, 1185
BstMAI	GTCTC	9 cut(s)	34, 413, 726, 1109, 1127, 1330, 1585, 1810, 1974
BstMCI	CGRYCG	2 cut(s)	48, 128
BstMWI	GCNNNNNNNGC	6 cut(s)	323, 1078, 1199, 1801, 2010, 2019
BstNI	CCWGG	1 cut(s)	1474
BstNSI	RCATGY	1 cut(s)	1541
BstSCI	CCNGG	2 cut(s)	972, 1472
BstSFI	CTRYAG	1 cut(s)	1167
BstSLI	GKGCMC	2 cut(s)	1204, 1961
BstUI	CGCG	2 cut(s)	1110, 1185
BstV1I	GCAGC	3 cut(s)	271, 936, 1319
BstV2I	GAAGAC	3 cut(s)	55, 526, 617
BstX2I	RGATCY	4 cut(s)	115, 1022, 1171, 1348
BstYI	RGATCY	4 cut(s)	115, 1022, 1171, 1348
BstZI	CGGCCG	1 cut(s)	125
Bsu15I	ATCGAT	2 cut(s)	48, 1648
BsuRI	GGCC	5 cut(s)	127, 295, 1373, 1570, 1959
BsuTUI	ATCGAT	2 cut(s)	48, 1648
BtgI	CCRYGG	2 cut(s)	331, 2039
BtrI	CACGTC	2 cut(s)	218, 1340
BtsCI	GGATG	3 cut(s)	529, 1744, 2016
BtsI	GCAGTG	1 cut(s)	1817
BtsIMutI	CAGTG	1 cut(s)	1817
Cac8I	GCNNGC	5 cut(s)	42, 297, 1256, 1539, 2024
CaiI	CAGNNNCTG	1 cut(s)	1873
Cfr13I	GGNCC	5 cut(s)	340, 1372, 1568, 1957, 1958
ClaI	ATCGAT	2 cut(s)	48, 1648
CseI	GACGC	3 cut(s)	46, 49, 664
Csp6I	GTAC	2 cut(s)	390, 1188
CspCI	CAANNNNNGTGG	2 cut(s)	1000, 1035
CviAII	CATG	7 cut(s)	332, 611, 676, 1064, 1529, 1538, 2006
CviQI	GTAC	2 cut(s)	390, 1188
DdeI	CTNAG	9 cut(s)	55, 233, 273, 515, 945, 984, 1131, 1212, 1564
EaeI	YGGCCR	1 cut(s)	125
EagI	CGGCCG	1 cut(s)	125
Eam1104I	CTCTTC	3 cut(s)	164, 179, 1164
EarI	CTCTTC	3 cut(s)	164, 179, 1164
EciI	GGCGGA	1 cut(s)	108
EclXI	CGGCCG	1 cut(s)	125
Eco130I	CCWWGG	1 cut(s)	331
Eco24I	GRGCYC	1 cut(s)	1961
Eco31I	GGTCTC	1 cut(s)	34
Eco47I	GGWCC	1 cut(s)	340
Eco52I	CGGCCG	1 cut(s)	125
Eco57I	CTGAAG	1 cut(s)	790
Eco88I	CYCGRG	3 cut(s)	887, 1764, 1954
EcoNI	CCTNNNNNAGG	1 cut(s)	1563
EcoO109I	RGGNCCY	2 cut(s)	340, 1958
EcoRI	GAATTC	1 cut(s)	1949
EcoRII	CCWGG	1 cut(s)	1472
EcoT14I	CCWWGG	1 cut(s)	331
EcoT22I	ATGCAT	2 cut(s)	729, 1543
EcoT38I	GRGCYC	1 cut(s)	1961
ErhI	CCWWGG	1 cut(s)	331
Esp3I	CGTCTC	1 cut(s)	1330
FaeI	CATG	7 cut(s)	335, 614, 679, 1067, 1532, 1541, 2009
FaqI	GGGAC	4 cut(s)	326, 1313, 1896, 1952
FatI	CATG	7 cut(s)	331, 610, 675, 1063, 1528, 1537, 2005
FauI	CCCGC	3 cut(s)	158, 1319, 1702
FauNDI	CATATG	1 cut(s)	1780
FbaI	TGATCA	1 cut(s)	700
Fnu4HI	GCNGC	4 cut(s)	285, 950, 1308, 1406
FokI	GGATG	3 cut(s)	516, 1751, 2003
FriOI	GRGCYC	1 cut(s)	1961
Fsp4HI	GCNGC	4 cut(s)	285, 950, 1308, 1406
FspBI	CTAG	4 cut(s)	222, 1197, 1272, 1674
GluI	GCNGC	4 cut(s)	285, 950, 1308, 1406
HaeIII	GGCC	5 cut(s)	127, 295, 1373, 1570, 1959
HapII	CCGG	2 cut(s)	476, 973
HgaI	GACGC	3 cut(s)	46, 49, 664
Hin1I	GRCGYC	3 cut(s)	38, 60, 656
Hin1II	CATG	7 cut(s)	335, 614, 679, 1067, 1532, 1541, 2009
HindIII	AAGCTT	2 cut(s)	1252, 1521
HpaII	CCGG	2 cut(s)	476, 973
HphI	GGTGA	4 cut(s)	332, 466, 1801, 1943
Hpy166II	GTNNAC	1 cut(s)	872
Hpy8I	GTNNAC	1 cut(s)	872
Hpy99I	CGWCG	2 cut(s)	133, 658
HpyAV	CCTTC	4 cut(s)	445, 545, 761, 1363
HpyCH4III	ACNGT	7 cut(s)	28, 996, 1017, 1595, 1875, 1995, 2040
HpyCH4IV	ACGT	6 cut(s)	131, 217, 312, 1339, 1626, 1828
HpyCH4V	TGCA	8 cut(s)	579, 727, 1000, 1067, 1492, 1541, 1642, 1847
HpyF10VI	GCNNNNNNNGC	6 cut(s)	323, 1078, 1199, 1801, 2010, 2019
HpyF3I	CTNAG	9 cut(s)	55, 233, 273, 515, 945, 984, 1131, 1212, 1564
HpySE526I	ACGT	6 cut(s)	131, 217, 312, 1339, 1626, 1828
Hsp92I	GRCGYC	3 cut(s)	38, 60, 656
Hsp92II	CATG	7 cut(s)	335, 614, 679, 1067, 1532, 1541, 2009
KflI	GGGWCCC	1 cut(s)	340
Ksp22I	TGATCA	1 cut(s)	700
LguI	GCTCTTC	1 cut(s)	179
LmnI	GCTCC	3 cut(s)	162, 607, 905
Lsp1109I	GCAGC	3 cut(s)	271, 936, 1319
LweI	GCATC	8 cut(s)	346, 714, 736, 917, 1303, 1459, 1550, 1856
MaeI	CTAG	4 cut(s)	222, 1197, 1272, 1674
MaeII	ACGT	6 cut(s)	131, 217, 312, 1339, 1626, 1828
MaeIII	GTNAC	6 cut(s)	34, 224, 671, 853, 1572, 1664
MbiI	CCGCTC	2 cut(s)	165, 189
MfeI	CAATTG	1 cut(s)	683
MflI	RGATCY	4 cut(s)	115, 1022, 1171, 1348
MhlI	GDGCHC	2 cut(s)	1204, 1961
MluCI	AATT	9 cut(s)	466, 647, 683, 1320, 1434, 1924, 1949, 2028, 2093
MlyI	GAGTC	7 cut(s)	187, 218, 221, 237, 850, 1784, 1878
MmeI	TCCRAC	2 cut(s)	80, 1977
Mph1103I	ATGCAT	2 cut(s)	729, 1543
MroXI	GAANNNNTTC	2 cut(s)	1158, 1422
MseI	TTAA	4 cut(s)	486, 525, 666, 776
MspI	CCGG	2 cut(s)	476, 973
MspR9I	CCNGG	2 cut(s)	974, 1474
MunI	CAATTG	1 cut(s)	683
Mva1269I	GAATGC	1 cut(s)	1697
MvaI	CCWGG	1 cut(s)	1474
MvnI	CGCG	2 cut(s)	1110, 1185
MwoI	GCNNNNNNNGC	6 cut(s)	323, 1078, 1199, 1801, 2010, 2019
NciI	CCSGG	1 cut(s)	974
NcoI	CCATGG	1 cut(s)	331
NdeI	CATATG	1 cut(s)	1780
NlaIII	CATG	7 cut(s)	335, 614, 679, 1067, 1532, 1541, 2009
NlaIV	GGNNCC	6 cut(s)	117, 341, 342, 1395, 1409, 1959
NmeAIII	GCCGAG	1 cut(s)	103
NmuCI	GTSAC	3 cut(s)	34, 224, 853
NsiI	ATGCAT	2 cut(s)	729, 1543
NspI	RCATGY	1 cut(s)	1541
NspV	TTCGAA	3 cut(s)	363, 746, 2032
PaeI	GCATGC	1 cut(s)	1541
PaeR7I	CTCGAG	1 cut(s)	1764
PciSI	GCTCTTC	1 cut(s)	179
PcsI	WCGNNNNNNNCGW	1 cut(s)	369
PctI	GAATGC	1 cut(s)	1697
PdmI	GAANNNNTTC	2 cut(s)	1158, 1422
PfeI	GAWTC	4 cut(s)	748, 1721, 1760, 1835
PkrI	GCNGC	4 cut(s)	286, 951, 1309, 1407
Ple19I	CGATCG	1 cut(s)	48
PleI	GAGTC	7 cut(s)	186, 217, 221, 237, 850, 1783, 1878
PpsI	GAGTC	7 cut(s)	186, 217, 221, 237, 850, 1783, 1878
PpuMI	RGGWCCY	1 cut(s)	340
Psp1406I	AACGTT	1 cut(s)	312
Psp5II	RGGWCCY	1 cut(s)	340
Psp6I	CCWGG	1 cut(s)	1472
PspGI	CCWGG	1 cut(s)	1472
PspN4I	GGNNCC	6 cut(s)	117, 341, 342, 1395, 1409, 1959
PspOMI	GGGCCC	1 cut(s)	1957
PspPI	GGNCC	5 cut(s)	340, 1372, 1568, 1957, 1958
PspPPI	RGGWCCY	1 cut(s)	340
PstNI	CAGNNNCTG	1 cut(s)	1873
PsuI	RGATCY	4 cut(s)	115, 1022, 1171, 1348
PvuI	CGATCG	1 cut(s)	48
RsaI	GTAC	2 cut(s)	391, 1189
RsaNI	GTAC	2 cut(s)	390, 1188
SapI	GCTCTTC	1 cut(s)	179
SaqAI	TTAA	4 cut(s)	486, 525, 666, 776
SatI	GCNGC	4 cut(s)	285, 950, 1308, 1406
Sau96I	GGNCC	5 cut(s)	340, 1372, 1568, 1957, 1958
SchI	GAGTC	7 cut(s)	187, 218, 221, 237, 850, 1784, 1878
ScrFI	CCNGG	2 cut(s)	974, 1474
SduI	GDGCHC	2 cut(s)	1204, 1961
SfaNI	GCATC	8 cut(s)	346, 714, 736, 917, 1303, 1459, 1550, 1856
SfcI	CTRYAG	1 cut(s)	1167
Sfr274I	CTCGAG	1 cut(s)	1764
SfuI	TTCGAA	3 cut(s)	363, 746, 2032
SinI	GGWCC	1 cut(s)	340
SlaI	CTCGAG	1 cut(s)	1764
SmlI	CTYRAG	3 cut(s)	1249, 1657, 1764
SmoI	CTYRAG	3 cut(s)	1249, 1657, 1764
SphI	GCATGC	1 cut(s)	1541
Sse9I	AATT	9 cut(s)	466, 647, 683, 1320, 1434, 1924, 1949, 2028, 2093
SspMI	CTAG	4 cut(s)	222, 1197, 1272, 1674
StyD4I	CCNGG	2 cut(s)	972, 1472
StyI	CCWWGG	1 cut(s)	331
TaaI	ACNGT	7 cut(s)	28, 996, 1017, 1595, 1875, 1995, 2040
TaiI	ACGT	6 cut(s)	134, 220, 315, 1342, 1629, 1831
TasI	AATT	9 cut(s)	466, 647, 683, 1320, 1434, 1924, 1949, 2028, 2093
TauI	GCSGC	1 cut(s)	1408
TfiI	GAWTC	4 cut(s)	748, 1721, 1760, 1835
Tru1I	TTAA	4 cut(s)	486, 525, 666, 776
Tru9I	TTAA	4 cut(s)	486, 525, 666, 776
TscAI	CASTG	1 cut(s)	1817
TseFI	GTSAC	3 cut(s)	34, 224, 853
TseI	GCWGC	3 cut(s)	284, 949, 1307
Tsp45I	GTSAC	3 cut(s)	34, 224, 853
TspDTI	ATGAA	9 cut(s)	90, 692, 780, 1368, 1439, 1755, 1914, 2004, 2022
TspGWI	ACGGA	1 cut(s)	911
TspRI	CASTG	1 cut(s)	1817
VpaK11BI	GGWCC	1 cut(s)	340
XagI	CCTNNNNNAGG	1 cut(s)	1563
XapI	RAATTY	2 cut(s)	647, 1949
XceI	RCATGY	1 cut(s)	1541
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1038
XhoI	CTCGAG	1 cut(s)	1764
XmnI	GAANNNNTTC	2 cut(s)	1158, 1422
XspI	CTAG	4 cut(s)	222, 1197, 1272, 1674
Zsp2I	ATGCAT	2 cut(s)	729, 1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.