Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005948

Physical Location & Seq

Genomic Coordinates Reverse (-)

29311275 .. 29316610

Gene Structure 5336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1205700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGGGTTGCGTGAGCTCGAAGCAAGCGGTGTCGGTGACGCCGGCGTTCGACCACTCGGGCGCCTTCAGGGACAATGTGGGGAATTCGGGTCGGAGTCGGGTCGGGCTGGGGGAGGCGGAGAAGAAGAGTAAGAAGGGGGCTGAGTTGAGCGGGAACGAGTTGGGTGAGTCGGGAAGAGCGAGTTCGAATGGCGGTGGCGGGAATTATCACAAGAGCAATAATAGTTATTCCGAGTCTTTGAGTTTTAGATTGAGCAATCTGCAGAAGTATGTGGAGGGTGAGCAGGTGGCCGCCGGCTGGCCTACTTGGCTCAGCGCCGTTGCCGGCGAAGCCATTCAAGGGTGGGTCCCACTCCGTGCAGATGCATATGAGAAGTTAGAGAAAATTGGACAGGGTACATATAGTAGCGTGTTCCGAGCACGGGACCTTGAAACTGGGAAGATAGTTGCCTTGAAGAAGGTGCGGTTTGACAATTTTGAGCCTGAGAGCGTCAGGTTTATGGCACGAGAAATCTTGATTCTTAGAAGGCTTGACCATCCAAACGTCGTTAAACTGGAGGGCTTAATTACTTCCCGATTATCATGTAGCATATACCTTGTGTTCGAGTACATGGAACATGACATTACCGGACTCTTGTCTAACCCTGACATCAAATTCAGTGAGGCACAGATTAAATGCTACATGAAGCAGCTGTTATCTGGACTTGAGCATTGTCACTCGCGGGGTGTAATGCATCGGGACGTAAAAGGATCAAATCTTCTGGTGAGCAATGAAGGAATTCTGAAGGTGGCTGATTTTGGGTTGGCAAACTTCTGTAATACCGGGCATAAGCAACCCCTAACCAGTCGTGTTGTAACTCTATGGTATCGTCCTCCTGAACTTTTGCTTGGATCGACTGATTATGGAGCTTTTATAGATCTATGGAGTGTTGGCTGTGCGTTTGCAGAGCTACTAATGGGGAAACCTGTTCTTCAAGGGAGAACAGAGGTTGAACAATTACACAAGATTTTCAAGCTTTGTGGCTCCCCGCCAGAAGATTACTGGAAAAAGTCCAAACTTCCTCATGCCACTCTATTTAAACCACAGCAACCTTACGATAGTTCTCTGCGGGATACCTTTAAAGATTTGCCTGCGGCTACTTTGAACCTACTAGAGACTCTCCTTTCTGTAGAACCACACAAGCGTGGGACTGCCTCCTCCGCTCTTGCATCCGAGTATTTCAAAATGAAGCCTCATGCATGCGATCCATCAAGCTTGCCTATGTACCCACCTAGCAAAGAGATAGATGCGAACAACCGGGAGGAGGCAAATAGGAAAAAAATTGGTGGGCGTGTTCGTGGATCTGAAACACGAAAGCCAGCAAGAAAGTCACTTGGGTTTAGTAAATTGGCACCACCAGAGGACTTGGCAGCTGAGTCTCGAGGTTCTTATAAAACTAATGGCACTAATGGACATATTCTTAAGGAAGATAACTCAATAGTAGGGAGGGAACTTCCAAAGCCGTCAGCTGGTAAAGTAGAAGACTCTTCGCATGTAAAAAATGCATCTCAAGGAGACATTCCACTCACCGGTCCATTAGAGGTTTCTACATCAAGTGGCTTTGCATGGGCAAAGAGACGAAAAGATGAGGCCAGAAGATCTCATACTCGGTCTATTTCAAGAGGACACATTTTTAACACGTTAGAATCTTCGATCACAGTGCATACAAGGAGCAACTTTGACTCAAGAAGGCATGAAAATGGGGAACTTACGTACAAGGGGTGCACTGATTCTAAAGGGAACGATTCATACGAGATTGCAAAGCTTGCAATGCTGAAACAATGGGGCAAGCTTGAGCGTCCAGATTCCTTTGATGCTTCCGAGGAGTACCATTCGCAGGAATTATCATTGGCACTTTATCAGAGAGAGGAAATGGCAGCTAAAAGTGATACAGGGTTTCAACTTCAAGATCAAGGAGAAAAGGTTGAATTTTCCGGACCCTTGTTGTCTCAACCGTATAGAGTTGATGAATTTTTGGACAGACATGAGCGCCGCATCAGAAGGGCGACCCGGAACTCATGGTTTCATAGAGGTAAAAAGCAGGGGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009966 GO:0009987 GO:0010646 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0031347 GO:0031349 GO:0032101 GO:0032103 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051726 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0097472 GO:0098542 GO:0140096 GO:1900424 GO:1900426 GO:1901564 GO:1902288 GO:1902290 GO:2000031 GO:2001023 GO:2001038

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

697

Amino Acids

77.54

Weight (kDa)

9.13

Isoelectric Point (pI)

43.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	123 - 407	6.7e-66	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	125 - 336	5.7e-29	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005948)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18670 AT1G18670 AT1G18670 AT1G74330 AT1G74330 AT1G74330
fragaria_vesca	FvH4_5g14470 FvH4_5g14470 FvH4_5g14470 FvH4_5g14470 FvH4_5g14470 FvH4_5g14470
malus_domestica	MD06G1198100.v1.1 MD14G1205700.v1.1
prunus_persica	Prupe.5G199000_v2.0.a1 Prupe.5G199000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0182181
rosa_laevigata	RLG00000005143
rosa_multiflora	Rmu_sc0000003.1_g000017
rosa_roxburghii	Rroxscaffold_3G00271440
rosa_rugosa	Rorug06G0445900 Rorug06G0445900
rosa_samantha	Rh7AG049000 Rh7BG048900 Rh7CG050300 Rh7DG048200
rosa_wichuraiana	Rw7G004000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1431
AarI	CACCTGC	1 cut(s)	274
Acc36I	ACCTGC	1 cut(s)	274
AccB1I	GGYRCC	2 cut(s)	59, 1390
AccBSI	CCGCTC	2 cut(s)	150, 1202
AccII	CGCG	1 cut(s)	721
AccIII	TCCGGA	1 cut(s)	1973
AclWI	GGATC	4 cut(s)	757, 898, 1238, 1348
AcoI	YGGCCR	1 cut(s)	288
AcsI	RAATTY	5 cut(s)	82, 653, 777, 1967, 2009
AcuI	CTGAAG	2 cut(s)	49, 803
AcyI	GRCGYC	2 cut(s)	38, 60
AdeI	CACNNNGTG	1 cut(s)	356
AfaI	GTAC	5 cut(s)	397, 608, 1265, 1754, 1868
AfiI	CCNNNNNNNGG	7 cut(s)	297, 339, 421, 1303, 1508, 1568, 1876
AflII	CTTAAG	1 cut(s)	1460
AflIII	ACRYGT	1 cut(s)	1677
AgeI	ACCGGT	1 cut(s)	1568
AjuI	GAANNNNNNNTTGG	2 cut(s)	870, 902
AleI	CACNNNNGTG	1 cut(s)	1182
Alw21I	GWGCWC	3 cut(s)	17, 421, 1766
Alw26I	GTCTC	5 cut(s)	1148, 1422, 1548, 1609, 1992
Alw44I	GTGCAC	1 cut(s)	1762
AlwI	GGATC	4 cut(s)	757, 898, 1238, 1348
Ama87I	CYCGRG	2 cut(s)	55, 1419
Aor13HI	TCCGGA	1 cut(s)	1973
AoxI	GGCC	3 cut(s)	288, 299, 1629
ApaLI	GTGCAC	1 cut(s)	1762
ApeKI	GCWGC	3 cut(s)	688, 1409, 1916
ApoI	RAATTY	5 cut(s)	82, 653, 777, 1967, 2009
AsiGI	ACCGGT	1 cut(s)	1568
Asp700I	GAANNNNTTC	2 cut(s)	333, 777
AspLEI	GCGC	3 cut(s)	62, 317, 2031
AspS9I	GGNCC	4 cut(s)	346, 424, 1571, 1976
AsuC2I	CCSGG	3 cut(s)	823, 1298, 2050
AsuHPI	GGTGA	5 cut(s)	46, 176, 290, 775, 1558
AsuII	TTCGAA	1 cut(s)	185
AvaI	CYCGRG	2 cut(s)	55, 1419
AvaII	GGWCC	4 cut(s)	346, 424, 1571, 1976
BaeGI	GKGCMC	1 cut(s)	1766
BanI	GGYRCC	2 cut(s)	59, 1390
BanII	GRGCYC	1 cut(s)	17
BarI	GAAGNNNNNNTAC	2 cut(s)	1627, 1659
BauI	CACGAG	1 cut(s)	504
BbsI	GAAGAC	1 cut(s)	1527
Bbv12I	GWGCWC	3 cut(s)	17, 421, 1766
BbvI	GCAGC	3 cut(s)	700, 1421, 1928
BccI	CCATC	2 cut(s)	543, 1255
BceAI	ACGGC	2 cut(s)	302, 1486
BcgI	CGANNNNNNTGC	2 cut(s)	1681, 1715
BciVI	GTATCC	1 cut(s)	1105
BcnI	CCSGG	3 cut(s)	823, 1298, 2050
BcoDI	GTCTC	5 cut(s)	1148, 1422, 1548, 1609, 1992
BfaI	CTAG	2 cut(s)	1151, 1272
BfmI	CTRYAG	2 cut(s)	260, 1167
BfoI	RGCGCY	3 cut(s)	63, 318, 2032
BfrI	CTTAAG	1 cut(s)	1460
BfuAI	ACCTGC	1 cut(s)	274
BfuI	GTATCC	1 cut(s)	1105
BglI	GCCNNNNNGGC	1 cut(s)	307
BglII	AGATCT	2 cut(s)	916, 1637
BisI	GCNGC	6 cut(s)	291, 689, 1134, 1410, 1917, 2032
BlpI	GCTNAGC	1 cut(s)	311
BlsI	GCNGC	6 cut(s)	292, 690, 1135, 1411, 1918, 2033
Bme1390I	CCNGG	3 cut(s)	823, 1298, 2050
Bme18I	GGWCC	4 cut(s)	346, 424, 1571, 1976
BmeT110I	CYCGRG	2 cut(s)	55, 1419
BmgT120I	GGNCC	4 cut(s)	346, 424, 1571, 1976
BmiI	GGNNCC	7 cut(s)	61, 347, 348, 425, 1024, 1392, 1978
BmrFI	CCNGG	3 cut(s)	823, 1298, 2050
BmrI	ACTGGG	1 cut(s)	444
BmsI	GCATC	7 cut(s)	352, 742, 1217, 1276, 1553, 1843, 2043
BmuI	ACTGGG	1 cut(s)	444
BoxI	GACNNNNGTC	1 cut(s)	634
BpiI	GAAGAC	1 cut(s)	1527
BpmI	CTGGAG	1 cut(s)	575
Bpu1102I	GCTNAGC	1 cut(s)	311
Bpu14I	TTCGAA	1 cut(s)	185
BpuEI	CTTGAG	4 cut(s)	725, 1533, 1708, 1853
BpuMI	CCSGG	3 cut(s)	823, 1298, 2050
BsaAI	YACGTR	1 cut(s)	1752
BsaHI	GRCGYC	2 cut(s)	38, 60
BsaJI	CCNNGG	1 cut(s)	1860
BsaWI	WCCGGW	3 cut(s)	626, 1568, 1973
BsaXI	ACNNNNNCTCC	2 cut(s)	1947, 1977
Bsc4I	CCNNNNNNNGG	7 cut(s)	297, 339, 421, 1303, 1508, 1568, 1876
Bse118I	RCCGGY	4 cut(s)	40, 293, 323, 1568
Bse1I	ACTGG	4 cut(s)	439, 558, 843, 1046
Bse3DI	GCAATG	2 cut(s)	775, 1815
BseAI	TCCGGA	1 cut(s)	1973
BseDI	CCNNGG	1 cut(s)	1860
BseGI	GGATG	2 cut(s)	535, 1208
BseLI	CCNNNNNNNGG	7 cut(s)	297, 339, 421, 1303, 1508, 1568, 1876
BseMI	GCAATG	2 cut(s)	775, 1815
BseMII	CTCAG	4 cut(s)	132, 325, 474, 1404
BseNI	ACTGG	4 cut(s)	439, 558, 843, 1046
BseRI	GAGGAG	3 cut(s)	1186, 1316, 1877
BseSI	GKGCMC	1 cut(s)	1766
BseXI	GCAGC	3 cut(s)	700, 1421, 1928
BseYI	CCCAGC	1 cut(s)	106
BsgI	GTGCAG	1 cut(s)	378
Bsh1236I	CGCG	1 cut(s)	721
BshFI	GGCC	3 cut(s)	290, 301, 1631
BshNI	GGYRCC	2 cut(s)	59, 1390
BshTI	ACCGGT	1 cut(s)	1568
BsiHKAI	GWGCWC	3 cut(s)	17, 421, 1766
BsiHKCI	CYCGRG	2 cut(s)	55, 1419
BsiSI	CCGG	9 cut(s)	41, 294, 324, 627, 822, 1297, 1569, 1974, 2050
BslFI	GGGAC	5 cut(s)	83, 332, 437, 752, 1201
BslI	CCNNNNNNNGG	7 cut(s)	297, 339, 421, 1303, 1508, 1568, 1876
BsmAI	GTCTC	5 cut(s)	1148, 1422, 1548, 1609, 1992
BsmBI	CGTCTC	1 cut(s)	1609
BsmFI	GGGAC	5 cut(s)	83, 332, 437, 752, 1201
BsnI	GGCC	3 cut(s)	290, 301, 1631
BsoBI	CYCGRG	2 cut(s)	55, 1419
Bsp119I	TTCGAA	1 cut(s)	185
Bsp1286I	GDGCHC	3 cut(s)	17, 421, 1766
Bsp13I	TCCGGA	1 cut(s)	1973
Bsp143I	GATC	8 cut(s)	749, 890, 916, 1243, 1340, 1637, 1692, 1948
Bsp1720I	GCTNAGC	1 cut(s)	311
BspANI	GGCC	3 cut(s)	290, 301, 1631
BspCNI	CTCAG	4 cut(s)	133, 324, 475, 1405
BspEI	TCCGGA	1 cut(s)	1973
BspFNI	CGCG	1 cut(s)	721
BspLI	GGNNCC	7 cut(s)	61, 347, 348, 425, 1024, 1392, 1978
BspMAI	CTGCAG	1 cut(s)	264
BspMI	ACCTGC	1 cut(s)	274
BspPI	GGATC	4 cut(s)	757, 898, 1238, 1348
BspQI	GCTCTTC	1 cut(s)	169
BspT104I	TTCGAA	1 cut(s)	185
BspT107I	GGYRCC	2 cut(s)	59, 1390
BspTI	CTTAAG	1 cut(s)	1460
BsrBI	CCGCTC	2 cut(s)	150, 1202
BsrDI	GCAATG	2 cut(s)	775, 1815
BsrFI	RCCGGY	4 cut(s)	40, 293, 323, 1568
BsrI	ACTGG	4 cut(s)	439, 558, 843, 1046
BssAI	RCCGGY	4 cut(s)	40, 293, 323, 1568
BssECI	CCNNGG	1 cut(s)	1860
BssMI	GATC	8 cut(s)	749, 890, 916, 1243, 1340, 1637, 1692, 1948
BssNI	GRCGYC	2 cut(s)	38, 60
BssSI	CACGAG	1 cut(s)	504
Bst2BI	CACGAG	1 cut(s)	504
Bst4CI	ACNGT	2 cut(s)	1699, 1995
Bst6I	CTCTTC	3 cut(s)	119, 169, 1531
BstACI	GRCGYC	2 cut(s)	38, 60
BstAFI	CTTAAG	1 cut(s)	1460
BstAPI	GCANNNNNTGC	1 cut(s)	1805
BstBAI	YACGTR	1 cut(s)	1752
BstBI	TTCGAA	1 cut(s)	185
BstDEI	CTNAG	5 cut(s)	141, 311, 483, 521, 1413
BstF5I	GGATG	2 cut(s)	535, 1208
BstFNI	CGCG	1 cut(s)	721
BstH2I	RGCGCY	3 cut(s)	63, 318, 2032
BstHHI	GCGC	3 cut(s)	62, 317, 2031
BstKTI	GATC	8 cut(s)	752, 893, 919, 1246, 1343, 1640, 1695, 1951
BstMAI	GTCTC	5 cut(s)	1148, 1422, 1548, 1609, 1992
BstMBI	GATC	8 cut(s)	749, 890, 916, 1243, 1340, 1637, 1692, 1948
BstMWI	GCNNNNNNNGC	5 cut(s)	307, 329, 1199, 1805, 1810
BstNSI	RCATGY	2 cut(s)	1242, 1535
BstPAI	GACNNNNGTC	1 cut(s)	634
BstSCI	CCNGG	3 cut(s)	821, 1296, 2048
BstSFI	CTRYAG	2 cut(s)	260, 1167
BstSLI	GKGCMC	1 cut(s)	1766
BstSNI	TACGTA	1 cut(s)	1752
BstUI	CGCG	1 cut(s)	721
BstV1I	GCAGC	3 cut(s)	700, 1421, 1928
BstV2I	GAAGAC	1 cut(s)	1527
BstX2I	RGATCY	3 cut(s)	916, 1340, 1637
BstYI	RGATCY	3 cut(s)	916, 1340, 1637
BsuI	GTATCC	1 cut(s)	1105
BsuRI	GGCC	3 cut(s)	290, 301, 1631
BtsCI	GGATG	2 cut(s)	535, 1208
BtsIMutI	CAGTG	3 cut(s)	664, 1704, 1764
BveI	ACCTGC	1 cut(s)	274
CfoI	GCGC	3 cut(s)	62, 317, 2031
Cfr10I	RCCGGY	4 cut(s)	40, 293, 323, 1568
Cfr13I	GGNCC	4 cut(s)	346, 424, 1571, 1976
CseI	GACGC	3 cut(s)	46, 478, 1826
Csp6I	GTAC	5 cut(s)	396, 607, 1264, 1753, 1867
CspAI	ACCGGT	1 cut(s)	1568
CspCI	CAANNNNNGTGG	2 cut(s)	1000, 1035
CviQI	GTAC	5 cut(s)	396, 607, 1264, 1753, 1867
DdeI	CTNAG	5 cut(s)	141, 311, 483, 521, 1413
DinI	GGCGCC	1 cut(s)	61
DpnI	GATC	8 cut(s)	751, 892, 918, 1245, 1342, 1639, 1694, 1950
DpnII	GATC	8 cut(s)	749, 890, 916, 1243, 1340, 1637, 1692, 1948
DraI	TTTAAA	2 cut(s)	1078, 1120
DraIII	CACNNNGTG	1 cut(s)	356
EaeI	YGGCCR	1 cut(s)	288
Eam1104I	CTCTTC	3 cut(s)	119, 169, 1531
EarI	CTCTTC	3 cut(s)	119, 169, 1531
EciI	GGCGGA	1 cut(s)	131
Ecl136II	GAGCTC	1 cut(s)	15
Eco105I	TACGTA	1 cut(s)	1752
Eco24I	GRGCYC	1 cut(s)	17
Eco47I	GGWCC	4 cut(s)	346, 424, 1571, 1976
Eco53kI	GAGCTC	1 cut(s)	15
Eco57I	CTGAAG	2 cut(s)	49, 803
Eco88I	CYCGRG	2 cut(s)	55, 1419
EcoICRI	GAGCTC	1 cut(s)	15
EcoO109I	RGGNCCY	2 cut(s)	346, 424
EcoRI	GAATTC	2 cut(s)	82, 777
EcoT22I	ATGCAT	4 cut(s)	367, 735, 1240, 1546
EcoT38I	GRGCYC	1 cut(s)	17
EgeI	GGCGCC	1 cut(s)	61
EheI	GGCGCC	1 cut(s)	61
Esp3I	CGTCTC	1 cut(s)	1609
FaqI	GGGAC	5 cut(s)	83, 332, 437, 752, 1201
FauI	CCCGC	5 cut(s)	143, 191, 714, 1035, 1101
FauNDI	CATATG	1 cut(s)	367
Fnu4HI	GCNGC	6 cut(s)	291, 689, 1134, 1410, 1917, 2032
FokI	GGATG	2 cut(s)	522, 1195
FriOI	GRGCYC	1 cut(s)	17
Fsp4HI	GCNGC	6 cut(s)	291, 689, 1134, 1410, 1917, 2032
FspBI	CTAG	2 cut(s)	1151, 1272
GlaI	GCGC	3 cut(s)	61, 316, 2030
GluI	GCNGC	6 cut(s)	291, 689, 1134, 1410, 1917, 2032
GsaI	CCCAGC	1 cut(s)	110
GsuI	CTGGAG	1 cut(s)	575
HaeII	RGCGCY	3 cut(s)	63, 318, 2032
HaeIII	GGCC	3 cut(s)	290, 301, 1631
HapII	CCGG	9 cut(s)	41, 294, 324, 627, 822, 1297, 1569, 1974, 2050
HgaI	GACGC	3 cut(s)	46, 478, 1826
HhaI	GCGC	3 cut(s)	62, 317, 2031
Hin1I	GRCGYC	2 cut(s)	38, 60
Hin6I	GCGC	3 cut(s)	60, 315, 2029
HinP1I	GCGC	3 cut(s)	60, 315, 2029
HindIII	AAGCTT	4 cut(s)	1013, 1252, 1802, 1829
HpaII	CCGG	9 cut(s)	41, 294, 324, 627, 822, 1297, 1569, 1974, 2050
HphI	GGTGA	5 cut(s)	46, 176, 290, 775, 1558
Hpy166II	GTNNAC	1 cut(s)	1764
Hpy188I	TCNGA	9 cut(s)	93, 232, 416, 783, 1213, 1345, 1861, 1902, 2039
Hpy8I	GTNNAC	1 cut(s)	1764
Hpy99I	CGWCG	1 cut(s)	548
HpyAV	CCTTC	8 cut(s)	73, 127, 451, 519, 767, 778, 1722, 2034
HpyCH4III	ACNGT	2 cut(s)	1699, 1995
HpyCH4IV	ACGT	4 cut(s)	543, 741, 1679, 1751
HpyF10VI	GCNNNNNNNGC	5 cut(s)	307, 329, 1199, 1805, 1810
HpyF3I	CTNAG	5 cut(s)	141, 311, 483, 521, 1413
HpySE526I	ACGT	4 cut(s)	543, 741, 1679, 1751
Hsp92I	GRCGYC	2 cut(s)	38, 60
HspAI	GCGC	3 cut(s)	60, 315, 2029
KasI	GGCGCC	1 cut(s)	59
KflI	GGGWCCC	1 cut(s)	346
Kpn2I	TCCGGA	1 cut(s)	1973
KroI	GCCGGC	3 cut(s)	40, 293, 323
KroNI	GCCGGC	3 cut(s)	42, 295, 325
Kzo9I	GATC	8 cut(s)	749, 890, 916, 1243, 1340, 1637, 1692, 1948
LguI	GCTCTTC	1 cut(s)	169
LmnI	GCTCC	3 cut(s)	905, 1028, 1710
Lsp1109I	GCAGC	3 cut(s)	700, 1421, 1928
LweI	GCATC	7 cut(s)	352, 742, 1217, 1276, 1553, 1843, 2043
MaeI	CTAG	2 cut(s)	1151, 1272
MaeII	ACGT	4 cut(s)	543, 741, 1679, 1751
MaeIII	GTNAC	4 cut(s)	34, 713, 853, 1368
MalI	GATC	8 cut(s)	751, 892, 918, 1245, 1342, 1639, 1694, 1950
MbiI	CCGCTC	2 cut(s)	150, 1202
MboI	GATC	8 cut(s)	749, 890, 916, 1243, 1340, 1637, 1692, 1948
MflI	RGATCY	3 cut(s)	916, 1340, 1637
MhlI	GDGCHC	3 cut(s)	17, 421, 1766
Mly113I	GGCGCC	1 cut(s)	60
MlyI	GAGTC	8 cut(s)	103, 176, 242, 624, 1150, 1424, 1517, 1715
MmeI	TCCRAC	1 cut(s)	71
Mph1103I	ATGCAT	4 cut(s)	367, 735, 1240, 1546
MreI	CGCCGGCG	1 cut(s)	40
MroI	TCCGGA	1 cut(s)	1973
MroNI	GCCGGC	3 cut(s)	40, 293, 323
MroXI	GAANNNNTTC	2 cut(s)	333, 777
MseI	TTAA	7 cut(s)	549, 563, 672, 1077, 1119, 1461, 1674
MslI	CAYNNNNRTG	2 cut(s)	1182, 1737
MspA1I	CMGCKG	3 cut(s)	691, 1412, 1508
MspCI	CTTAAG	1 cut(s)	1460
MspI	CCGG	9 cut(s)	41, 294, 324, 627, 822, 1297, 1569, 1974, 2050
MspR9I	CCNGG	3 cut(s)	823, 1298, 2050
MvnI	CGCG	1 cut(s)	721
MwoI	GCNNNNNNNGC	5 cut(s)	307, 329, 1199, 1805, 1810
NaeI	GCCGGC	3 cut(s)	42, 295, 325
NarI	GGCGCC	1 cut(s)	60
NciI	CCSGG	3 cut(s)	823, 1298, 2050
NdeI	CATATG	1 cut(s)	367
NdeII	GATC	8 cut(s)	749, 890, 916, 1243, 1340, 1637, 1692, 1948
NgoMIV	GCCGGC	3 cut(s)	40, 293, 323
NlaIV	GGNNCC	7 cut(s)	61, 347, 348, 425, 1024, 1392, 1978
NmuCI	GTSAC	3 cut(s)	34, 713, 1368
NsiI	ATGCAT	4 cut(s)	367, 735, 1240, 1546
NspI	RCATGY	2 cut(s)	1242, 1535
NspV	TTCGAA	1 cut(s)	185
OliI	CACNNNNGTG	1 cut(s)	1182
PaeI	GCATGC	1 cut(s)	1242
PaeR7I	CTCGAG	1 cut(s)	1419
PaqCI	CACCTGC	1 cut(s)	274
PciSI	GCTCTTC	1 cut(s)	169
PcsI	WCGNNNNNNNCGW	2 cut(s)	176, 1788
PdiI	GCCGGC	3 cut(s)	42, 295, 325
PdmI	GAANNNNTTC	2 cut(s)	333, 777
PfeI	GAWTC	5 cut(s)	517, 1685, 1769, 1784, 1844
PinAI	ACCGGT	1 cut(s)	1568
PkrI	GCNGC	6 cut(s)	292, 690, 1135, 1411, 1918, 2033
PleI	GAGTC	8 cut(s)	102, 175, 241, 624, 1150, 1423, 1517, 1715
PluTI	GGCGCC	1 cut(s)	63
PpsI	GAGTC	8 cut(s)	102, 175, 241, 624, 1150, 1423, 1517, 1715
Ppu21I	YACGTR	1 cut(s)	1752
PpuMI	RGGWCCY	2 cut(s)	346, 424
PshAI	GACNNNNGTC	1 cut(s)	634
PsiI	TTATAA	1 cut(s)	1431
Psp124BI	GAGCTC	1 cut(s)	17
Psp5II	RGGWCCY	2 cut(s)	346, 424
PspFI	CCCAGC	1 cut(s)	106
PspN4I	GGNNCC	7 cut(s)	61, 347, 348, 425, 1024, 1392, 1978
PspPI	GGNCC	4 cut(s)	346, 424, 1571, 1976
PspPPI	RGGWCCY	2 cut(s)	346, 424
PsrI	GAACNNNNNNTAC	2 cut(s)	1772, 1804
PstI	CTGCAG	1 cut(s)	264
PsuI	RGATCY	3 cut(s)	916, 1340, 1637
PvuII	CAGCTG	3 cut(s)	691, 1412, 1508
RsaI	GTAC	5 cut(s)	397, 608, 1265, 1754, 1868
RsaNI	GTAC	5 cut(s)	396, 607, 1264, 1753, 1867
RseI	CAYNNNNRTG	2 cut(s)	1182, 1737
SacI	GAGCTC	1 cut(s)	17
SapI	GCTCTTC	1 cut(s)	169
SaqAI	TTAA	7 cut(s)	549, 563, 672, 1077, 1119, 1461, 1674
SatI	GCNGC	6 cut(s)	291, 689, 1134, 1410, 1917, 2032
Sau3AI	GATC	8 cut(s)	749, 890, 916, 1243, 1340, 1637, 1692, 1948
Sau96I	GGNCC	4 cut(s)	346, 424, 1571, 1976
SchI	GAGTC	8 cut(s)	103, 176, 242, 624, 1150, 1424, 1517, 1715
ScrFI	CCNGG	3 cut(s)	823, 1298, 2050
SduI	GDGCHC	3 cut(s)	17, 421, 1766
SfaNI	GCATC	7 cut(s)	352, 742, 1217, 1276, 1553, 1843, 2043
SfcI	CTRYAG	2 cut(s)	260, 1167
SfoI	GGCGCC	1 cut(s)	61
Sfr274I	CTCGAG	1 cut(s)	1419
SfuI	TTCGAA	1 cut(s)	185
SgrAI	CRCCGGYG	1 cut(s)	40
SinI	GGWCC	4 cut(s)	346, 424, 1571, 1976
SlaI	CTCGAG	1 cut(s)	1419
SmiMI	CAYNNNNRTG	2 cut(s)	1182, 1737
SmlI	CTYRAG	6 cut(s)	704, 1419, 1460, 1548, 1723, 1832
SmoI	CTYRAG	6 cut(s)	704, 1419, 1460, 1548, 1723, 1832
SnaBI	TACGTA	1 cut(s)	1752
SphI	GCATGC	1 cut(s)	1242
SspDI	GGCGCC	1 cut(s)	59
SspMI	CTAG	2 cut(s)	1151, 1272
SstI	GAGCTC	1 cut(s)	17
StyD4I	CCNGG	3 cut(s)	821, 1296, 2048
TaaI	ACNGT	2 cut(s)	1699, 1995
TaiI	ACGT	4 cut(s)	546, 744, 1682, 1754
TaqI	TCGA	7 cut(s)	17, 48, 185, 603, 893, 1420, 1691
TaqII	GACCGA	1 cut(s)	1638
TatI	WGTACW	1 cut(s)	606
TauI	GCSGC	3 cut(s)	293, 1136, 2034
TfiI	GAWTC	5 cut(s)	517, 1685, 1769, 1784, 1844
Tru1I	TTAA	7 cut(s)	549, 563, 672, 1077, 1119, 1461, 1674
Tru9I	TTAA	7 cut(s)	549, 563, 672, 1077, 1119, 1461, 1674
TscAI	CASTG	3 cut(s)	664, 1704, 1771
TseFI	GTSAC	3 cut(s)	34, 713, 1368
TseI	GCWGC	3 cut(s)	688, 1409, 1916
Tsp45I	GTSAC	3 cut(s)	34, 713, 1368
TspDTI	ATGAA	7 cut(s)	698, 786, 1241, 1749, 1776, 2022, 2054
TspGWI	ACGGA	1 cut(s)	344
TspRI	CASTG	3 cut(s)	664, 1704, 1771
Vha464I	CTTAAG	1 cut(s)	1460
VneI	GTGCAC	1 cut(s)	1762
VpaK11BI	GGWCC	4 cut(s)	346, 424, 1571, 1976
XapI	RAATTY	5 cut(s)	82, 653, 777, 1967, 2009
XceI	RCATGY	2 cut(s)	1242, 1535
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1038
XhoI	CTCGAG	1 cut(s)	1419
XmnI	GAANNNNTTC	2 cut(s)	333, 777
XspI	CTAG	2 cut(s)	1151, 1272
Zsp2I	ATGCAT	4 cut(s)	367, 735, 1240, 1546

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.