AT1G76250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011290
Physical Location & Seq
Reverse (-)
28606444 .. 28609225
2782 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76250.1

Sequence Viewer

Length: 1305 bp
ATGTACGTTTCCAGGAGAATCTCCGACGTCGATCGTCGTCTTCTCCTCCTTCTCATAATCCCATCTCTCACCCTCCTCGTCTTAATCTCTTTCTCATCTCTCTCACTTGATCCTTTTCCAACTCTAGCCCCTCTTCGTAACCTAATCTACACTCATACCCTAACCGTCACCGATTTCACCGTCGAATTGTCAGATTCGAACCCTAACGACGTTATCCAAGCGGAGGAGATGGAAAATCGACGGCGGAGGAAGAGGGAGGAGTTGGTTAAGTCGAAGATTGCGGTGTGTTTGGTAGGAGGAGCGAGGCGGTTTGAGTTAACCGGACCGTCGATTATGGAGAAGATCTTGAGGGTTTATCCTAATGCTGATTTGTTTCTTAATAGTCCTCTCGACAAAAATTCTTTCAAACTTCGGTTACTTAAGGACGCACCAAGACTTGCTTGGGTTCGTATCTTTGAGCCTAAGCCAATCAATGAGACTGAGCCGATGGTTCGAGTCCTCACACCTATGAATTCGCCCAATGGCATTAAGGGCTTATTACAGTACTTCAACCTTGTGGAAGGTTGCATCACGATGATAAAGGCATACCAAAACGAGAATAACTTCACATACGACTGGATAGTCCGGACCCGAGTTGATGGTTACTGGGCTGACCCGCTCGACCCGGAATACTTCATACCGGGTCAATACCTAGTTCCACCTGGATCCTCTTATGGTGGTCTCAACGACCGTTTCGGCGTCGGAGATCTCAACACCTCGACGGTAGCTCTCTCTCGCCTCTCCCTCATTCCCGACCTCGATTCAGCCGGGTTAACTCATCTGAACTCCGAGTCCGCTTTCAAGGCTCAGCTCACAACCCAACGTGTTCCTTACGTCACCAAACCTCTCCCTTTCTGCATCATGACTGATCGGACCTACGATTTCCCTCCATATAGATACGGAGTTCCAGTGGCGGCACTATCGAGCCGTGGACCGTTAAACGGTGCCAAGTGCCGTCCTTGCACGGTCGCATGCAACGGCTCTTGCGTGGCAGAAGTGATGGGAAAGCTGAATAAAGAGTGGAGCTGGACAGAGTGGGAAAACGAGACGGTGGAGCTGTGCGACGCACATGGAGAATGGGAGAAAGGTTGGGAGAAGATTTTTGACGAAACCGCTGGAGAAAAACTCGCACTTGCGAGGAAACGAGTCGGTGGTTTGGATTCGAGGAGATGTGTGGAAGAGTTTGAAAATATGAGAGGAATGGCCGTTAAATGGGAAGCTCCCGCCTCCGAACAGATTTGTACTTTGGGCTTACGGCCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

434

Amino Acids

49.07

Weight (kDa)

6.41

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7796 PF25072 90 - 432 1.1e-180 Domain of unknown function (DUF7796)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 620
AatII GACGTC 1 cut(s) 30
AccB1I GGYRCC 1 cut(s) 983
AccBSI CCGCTC 1 cut(s) 658
AccIII TCCGGA 1 cut(s) 624
AciI CCGC 9 cut(s) 221, 244, 281, 307, 656, 834, 953, 1152, 1263
AclWI GGATC 3 cut(s) 104, 699, 712
AcoI YGGCCR 1 cut(s) 1242
AcsI RAATTY 2 cut(s) 397, 511
AcyI GRCGYC 2 cut(s) 27, 738
AfaI GTAC 3 cut(s) 5, 545, 1282
AfiI CCNNNNNNNGG 3 cut(s) 223, 980, 1251
AflII CTTAAG 1 cut(s) 419
AflIII ACRYGT 1 cut(s) 862
AgsI TTSAA 4 cut(s) 406, 550, 841, 1226
AjnI CCWGG 2 cut(s) 11, 700
AloI GAACNNNNNNTCC 2 cut(s) 815, 847
AluBI AGCT 6 cut(s) 767, 850, 1048, 1065, 1096, 1259
AluI AGCT 6 cut(s) 767, 850, 1048, 1065, 1096, 1259
Alw26I GTCTC 3 cut(s) 470, 725, 1079
AlwI GGATC 3 cut(s) 104, 699, 712
Ama87I CYCGRG 1 cut(s) 630
Aor13HI TCCGGA 1 cut(s) 624
AoxI GGCC 2 cut(s) 1242, 1295
ApoI RAATTY 2 cut(s) 397, 511
Asp700I GAANNNNTTC 1 cut(s) 602
AspS9I GGNCC 5 cut(s) 323, 627, 912, 971, 1296
AsuC2I CCSGG 3 cut(s) 665, 681, 808
AsuHPI GGTGA 4 cut(s) 61, 160, 169, 868
AsuII TTCGAA 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 4 cut(s) 323, 627, 912, 971
BamHI GGATCC 1 cut(s) 704
BanI GGYRCC 1 cut(s) 983
BbsI GAAGAC 1 cut(s) 32
BccI CCATC 5 cut(s) 70, 223, 481, 632, 1033
BceAI ACGGC 5 cut(s) 257, 951, 978, 1033, 1229
BciT130I CCWGG 2 cut(s) 13, 702
BcnI CCSGG 3 cut(s) 665, 681, 808
BcoDI GTCTC 3 cut(s) 470, 725, 1079
BfaI CTAG 2 cut(s) 125, 692
BfrI CTTAAG 1 cut(s) 419
BglII AGATCT 2 cut(s) 342, 745
BisI GCNGC 1 cut(s) 954
BlpI GCTNAGC 1 cut(s) 846
BlsI GCNGC 1 cut(s) 955
BmcAI AGTACT 1 cut(s) 545
Bme1390I CCNGG 5 cut(s) 13, 665, 681, 702, 808
Bme18I GGWCC 4 cut(s) 323, 627, 912, 971
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 5 cut(s) 323, 627, 912, 971, 1296
BmiI GGNNCC 3 cut(s) 629, 706, 985
BmrFI CCNGG 5 cut(s) 13, 665, 681, 702, 808
BmrI ACTGGG 1 cut(s) 655
BmsI GCATC 2 cut(s) 576, 906
BmuI ACTGGG 1 cut(s) 655
BpiI GAAGAC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 1149, 1181
BpmI CTGGAG 1 cut(s) 1176
Bpu10I CCTNAGC 1 cut(s) 462
Bpu1102I GCTNAGC 1 cut(s) 846
Bpu14I TTCGAA 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 367
BpuMI CCSGG 3 cut(s) 665, 681, 808
BsaHI GRCGYC 2 cut(s) 27, 738
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 1 cut(s) 967
BsaWI WCCGGW 2 cut(s) 320, 624
BsaXI ACNNNNNCTCC 2 cut(s) 933, 963
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 980, 1251
Bse1I ACTGG 3 cut(s) 620, 650, 947
BseAI TCCGGA 1 cut(s) 624
BseBI CCWGG 2 cut(s) 13, 702
BseDI CCNNGG 1 cut(s) 967
BseLI CCNNNNNNNGG 3 cut(s) 223, 980, 1251
BseMII CTCAG 2 cut(s) 471, 860
BseNI ACTGG 3 cut(s) 620, 650, 947
BseRI GAGGAG 6 cut(s) 35, 65, 239, 272, 312, 1219
Bsh1285I CGRYCG 3 cut(s) 34, 730, 1008
BshFI GGCC 2 cut(s) 1244, 1297
BshNI GGYRCC 1 cut(s) 983
BsiEI CGRYCG 3 cut(s) 34, 730, 1008
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 5 cut(s) 321, 625, 665, 680, 807
BslI CCNNNNNNNGG 3 cut(s) 223, 980, 1251
BsmAI GTCTC 3 cut(s) 470, 725, 1079
BsmBI CGTCTC 1 cut(s) 1079
BsnI GGCC 2 cut(s) 1244, 1297
Bso31I GGTCTC 1 cut(s) 725
BsoBI CYCGRG 1 cut(s) 630
Bsp119I TTCGAA 1 cut(s) 197
Bsp13I TCCGGA 1 cut(s) 624
Bsp143I GATC 6 cut(s) 31, 109, 342, 704, 745, 907
Bsp1720I GCTNAGC 1 cut(s) 846
BspACI CCGC 9 cut(s) 221, 244, 281, 307, 656, 834, 953, 1152, 1263
BspANI GGCC 2 cut(s) 1244, 1297
BspCNI CTCAG 2 cut(s) 472, 859
BspEI TCCGGA 1 cut(s) 624
BspHI TCATGA 1 cut(s) 900
BspLI GGNNCC 3 cut(s) 629, 706, 985
BspPI GGATC 3 cut(s) 104, 699, 712
BspT104I TTCGAA 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 983
BspTI CTTAAG 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 725
BsrBI CCGCTC 1 cut(s) 658
BsrI ACTGG 3 cut(s) 620, 650, 947
BssECI CCNNGG 1 cut(s) 967
BssMI GATC 6 cut(s) 31, 109, 342, 704, 745, 907
BssNI GRCGYC 2 cut(s) 27, 738
Bst2UI CCWGG 2 cut(s) 13, 702
Bst6I CTCTTC 3 cut(s) 138, 245, 1212
BstACI GRCGYC 2 cut(s) 27, 738
BstAFI CTTAAG 1 cut(s) 419
BstBI TTCGAA 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 1012
BstDEI CTNAG 3 cut(s) 462, 480, 846
BstDSI CCRYGG 1 cut(s) 967
BstKTI GATC 6 cut(s) 34, 112, 345, 707, 748, 910
BstMAI GTCTC 3 cut(s) 470, 725, 1079
BstMBI GATC 6 cut(s) 31, 109, 342, 704, 745, 907
BstMCI CGRYCG 3 cut(s) 34, 730, 1008
BstMWI GCNNNNNNNGC 3 cut(s) 531, 842, 999
BstNI CCWGG 2 cut(s) 13, 702
BstNSI RCATGY 1 cut(s) 1014
BstSCI CCNGG 5 cut(s) 11, 663, 679, 700, 806
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 3 cut(s) 342, 704, 745
BstYI RGATCY 3 cut(s) 342, 704, 745
BsuRI GGCC 2 cut(s) 1244, 1297
BtgI CCRYGG 1 cut(s) 967
BtsIMutI CAGTG 1 cut(s) 954
Cac8I GCNNGC 1 cut(s) 1012
CciI TCATGA 1 cut(s) 900
Cfr13I GGNCC 5 cut(s) 323, 627, 912, 971, 1296
CpoI CGGWCCG 1 cut(s) 323
CseI GACGC 3 cut(s) 434, 727, 1112
Csp6I GTAC 3 cut(s) 4, 544, 1281
CspI CGGWCCG 1 cut(s) 323
CviAII CATG 3 cut(s) 901, 1011, 1109
CviQI GTAC 3 cut(s) 4, 544, 1281
DdeI CTNAG 3 cut(s) 462, 480, 846
DpnI GATC 6 cut(s) 33, 111, 344, 706, 747, 909
DpnII GATC 6 cut(s) 31, 109, 342, 704, 745, 907
DrdI GACNNNNNNGTC 1 cut(s) 620
DseDI GACNNNNNNGTC 1 cut(s) 620
EaeI YGGCCR 1 cut(s) 1242
Eam1104I CTCTTC 3 cut(s) 138, 245, 1212
EarI CTCTTC 3 cut(s) 138, 245, 1212
EciI GGCGGA 1 cut(s) 259
Eco31I GGTCTC 1 cut(s) 725
Eco47I GGWCC 4 cut(s) 323, 627, 912, 971
Eco88I CYCGRG 1 cut(s) 630
EcoRI GAATTC 1 cut(s) 511
EcoRII CCWGG 2 cut(s) 11, 700
Esp3I CGTCTC 1 cut(s) 1079
FaeI CATG 3 cut(s) 904, 1014, 1112
FalI AAGNNNNNCTT 2 cut(s) 424, 456
FatI CATG 3 cut(s) 900, 1010, 1108
FauI CCCGC 2 cut(s) 663, 1270
Fnu4HI GCNGC 1 cut(s) 954
Fsp4HI GCNGC 1 cut(s) 954
FspBI CTAG 2 cut(s) 125, 692
GluI GCNGC 1 cut(s) 954
GsuI CTGGAG 1 cut(s) 1176
HaeIII GGCC 2 cut(s) 1244, 1297
HapII CCGG 5 cut(s) 321, 625, 665, 680, 807
HgaI GACGC 3 cut(s) 434, 727, 1112
Hin1I GRCGYC 2 cut(s) 27, 738
Hin1II CATG 3 cut(s) 904, 1014, 1112
HincII GTYRAC 2 cut(s) 318, 813
HindII GTYRAC 2 cut(s) 318, 813
HinfI GANTC 7 cut(s) 18, 194, 495, 800, 830, 1185, 1199
HpaI GTTAAC 2 cut(s) 318, 813
HpaII CCGG 5 cut(s) 321, 625, 665, 680, 807
HphI GGTGA 4 cut(s) 61, 160, 169, 868
Hpy166II GTNNAC 3 cut(s) 318, 813, 971
Hpy188I TCNGA 7 cut(s) 25, 193, 743, 822, 829, 912, 1270
Hpy188III TCNNGA 6 cut(s) 346, 389, 571, 625, 791, 901
Hpy8I GTNNAC 3 cut(s) 318, 813, 971
HpyAV CCTTC 2 cut(s) 59, 554
HpyCH4IV ACGT 5 cut(s) 6, 27, 210, 862, 873
HpyCH4V TGCA 4 cut(s) 567, 897, 1002, 1014
HpyF10VI GCNNNNNNNGC 3 cut(s) 531, 842, 999
HpyF3I CTNAG 3 cut(s) 462, 480, 846
HpySE526I ACGT 5 cut(s) 6, 27, 210, 862, 873
Hsp92I GRCGYC 2 cut(s) 27, 738
Hsp92II CATG 3 cut(s) 904, 1014, 1112
Kpn2I TCCGGA 1 cut(s) 624
KspAI GTTAAC 2 cut(s) 318, 813
Kzo9I GATC 6 cut(s) 31, 109, 342, 704, 745, 907
LmnI GCTCC 4 cut(s) 299, 1062, 1093, 1264
LweI GCATC 2 cut(s) 576, 906
MaeI CTAG 2 cut(s) 125, 692
MaeII ACGT 5 cut(s) 6, 27, 210, 862, 873
MaeIII GTNAC 5 cut(s) 137, 166, 414, 641, 874
MalI GATC 6 cut(s) 33, 111, 344, 706, 747, 909
MbiI CCGCTC 1 cut(s) 658
MboI GATC 6 cut(s) 31, 109, 342, 704, 745, 907
MboII GAAGA 7 cut(s) 32, 125, 262, 286, 352, 1147, 1229
MflI RGATCY 3 cut(s) 342, 704, 745
MluCI AATT 4 cut(s) 185, 397, 511, 1300
MlyI GAGTC 3 cut(s) 504, 839, 1194
MmeI TCCRAC 3 cut(s) 48, 143, 721
MroI TCCGGA 1 cut(s) 624
MroXI GAANNNNTTC 1 cut(s) 602
MseI TTAA 9 cut(s) 83, 267, 317, 378, 420, 528, 812, 977, 1248
MslI CAYNNNNRTG 2 cut(s) 506, 572
MspA1I CMGCKG 1 cut(s) 1154
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 5 cut(s) 321, 625, 665, 680, 807
MspR9I CCNGG 5 cut(s) 13, 665, 681, 702, 808
MvaI CCWGG 2 cut(s) 13, 702
MwoI GCNNNNNNNGC 3 cut(s) 531, 842, 999
NciI CCSGG 3 cut(s) 665, 681, 808
NdeII GATC 6 cut(s) 31, 109, 342, 704, 745, 907
NlaIII CATG 3 cut(s) 904, 1014, 1112
NlaIV GGNNCC 3 cut(s) 629, 706, 985
NmuCI GTSAC 2 cut(s) 166, 874
NspI RCATGY 1 cut(s) 1014
NspV TTCGAA 1 cut(s) 197
PaeI GCATGC 1 cut(s) 1014
PagI TCATGA 1 cut(s) 900
PcsI WCGNNNNNNNCGW 1 cut(s) 1023
PdmI GAANNNNTTC 1 cut(s) 602
PfeI GAWTC 4 cut(s) 18, 194, 800, 1199
PfoI TCCNGGA 1 cut(s) 11
PkrI GCNGC 1 cut(s) 955
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 3 cut(s) 503, 838, 1193
PpsI GAGTC 3 cut(s) 503, 838, 1193
Psp6I CCWGG 2 cut(s) 11, 700
PspGI CCWGG 2 cut(s) 11, 700
PspN4I GGNNCC 3 cut(s) 629, 706, 985
PspPI GGNCC 5 cut(s) 323, 627, 912, 971, 1296
PsuI RGATCY 3 cut(s) 342, 704, 745
PvuI CGATCG 1 cut(s) 34
RsaI GTAC 3 cut(s) 5, 545, 1282
RsaNI GTAC 3 cut(s) 4, 544, 1281
RseI CAYNNNNRTG 2 cut(s) 506, 572
Rsr2I CGGWCCG 1 cut(s) 323
RsrII CGGWCCG 1 cut(s) 323
SaqAI TTAA 9 cut(s) 83, 267, 317, 378, 420, 528, 812, 977, 1248
SatI GCNGC 1 cut(s) 954
Sau3AI GATC 6 cut(s) 31, 109, 342, 704, 745, 907
Sau96I GGNCC 5 cut(s) 323, 627, 912, 971, 1296
ScaI AGTACT 1 cut(s) 545
SchI GAGTC 3 cut(s) 504, 839, 1194
ScrFI CCNGG 5 cut(s) 13, 665, 681, 702, 808
SfaNI GCATC 2 cut(s) 576, 906
SfuI TTCGAA 1 cut(s) 197
SinI GGWCC 4 cut(s) 323, 627, 912, 971
SmiMI CAYNNNNRTG 2 cut(s) 506, 572
SmlI CTYRAG 2 cut(s) 346, 419
SmoI CTYRAG 2 cut(s) 346, 419
SphI GCATGC 1 cut(s) 1014
Sse9I AATT 4 cut(s) 185, 397, 511, 1300
SsiI CCGC 9 cut(s) 221, 244, 281, 307, 656, 834, 953, 1152, 1263
SspMI CTAG 2 cut(s) 125, 692
StyD4I CCNGG 5 cut(s) 11, 663, 679, 700, 806
TaiI ACGT 5 cut(s) 9, 30, 213, 865, 876
TasI AATT 4 cut(s) 185, 397, 511, 1300
TatI WGTACW 2 cut(s) 543, 1280
TauI GCSGC 1 cut(s) 956
TfiI GAWTC 4 cut(s) 18, 194, 800, 1199
Tru1I TTAA 9 cut(s) 83, 267, 317, 378, 420, 528, 812, 977, 1248
Tru9I TTAA 9 cut(s) 83, 267, 317, 378, 420, 528, 812, 977, 1248
TscAI CASTG 1 cut(s) 954
TseFI GTSAC 2 cut(s) 166, 874
Tsp45I GTSAC 2 cut(s) 166, 874
TspDTI ATGAA 2 cut(s) 524, 664
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 1 cut(s) 954
Vha464I CTTAAG 1 cut(s) 419
VpaK11BI GGWCC 4 cut(s) 323, 627, 912, 971
XapI RAATTY 2 cut(s) 397, 511
XceI RCATGY 1 cut(s) 1014
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 2 cut(s) 125, 692
ZraI GACGTC 1 cut(s) 28
ZrmI AGTACT 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.