MD15G1002800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011290
Physical Location & Seq
Forward (+)
163449 .. 167804
4356 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1002800.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGAATTGGGTCACTCCAGAATCGCCATCTGCTTGGTTGGTGGGGCCCGAAAGTTCGAGCTCACGGGGCCGTCCATCCTCGACAAGATTCTCAACAAATATCTCAATTCCGATCTTTTCTTGCACAGTCCTGTTAACCCCAACGCCTTCAAGTTCTCGCTCCTCAAGTCGGCTCCAAGGATCGCCGCTGTCAAAATCTTCCACCCCAAAAGGATACCCGAGACCGAGTGCTAACCGCCAACAACTCGCCCAATGGCATCCAGGGATCCATGCTTTCGATAGGTTTGGAAGATGTGGTCTCTTGCAATATTTCAACCTGGTGGAAGGCTGCCTAACTATGATCCGAGCTTACCAAAAACAGAACAACTTCACCTACGATTGGATTGTCCGAACCCGCGTTGACGGTTACTGGAACGCCCCCCTCCACCCCAAGTACTTCGTGCCCGGTCAGTACCTGGTCCCACCAGGCTCCAGCTACGGCGGACTCAACGACCGCTTTGGAGTTGGCGACCTCAACACCTCCACTGTCGCCCTCTCGCGCCTCTCCCTCCTTCCCCAGCTCGACGCAGCCGGATTCCACCAACTAAACTCCGAAACCGCCTTCAAGGCCCAACTCACCATCCAAGGGGTACCTTACGTCACTAAACGACTCCCCTTTTGTATTGTAACAGACCGCAAGTACGAGTTCCCGCCACACCGCTTTGGAGTGCCGGTGGCGTCCTTGTCAAGCCCAGGGCCATTGAGCGGGGCCAAGTGCAGGCCTTGCACGCCCGTATGCCAAGGCCCGTGCGTGGCCGACGTGATGCAGTCTCTCTACAAAGGGTGGAGCTGGACCCACTGGGCAAACGGCACCCTTGAGCTCTGCGATGCCCATGACGCTTGGGAAAAGGATTGGGAGATCAAATTTGACCGAGTTGCTGGGAAGAAACTGGCTGCCGAGAGAAAGCGAGTTGCAGGCATGACGACAGAGCAATGCATTCATGATTTCAACCAAATGAAGAAGCGAACTGCCAACTGGGACGCACCCCCGGCAGAACAGATTTGTAGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

38.99

Weight (kDa)

9.31

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7796 PF25072 98 - 349 7.5e-137 Domain of unknown function (DUF7796)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 628
AccB1I GGYRCC 2 cut(s) 628, 848
AccBSI CCGCTC 1 cut(s) 744
AccII CGCG 2 cut(s) 396, 538
AclWI GGATC 4 cut(s) 187, 259, 272, 334
AcoI YGGCCR 1 cut(s) 792
AcsI RAATTY 1 cut(s) 902
AcyI GRCGYC 1 cut(s) 716
AfaI GTAC 4 cut(s) 434, 452, 630, 680
AfiI CCNNNNNNNGG 6 cut(s) 168, 378, 624, 743, 756, 790
AgsI TTSAA 4 cut(s) 150, 313, 604, 988
AjiI CACGTC 1 cut(s) 799
AjnI CCWGG 5 cut(s) 259, 315, 453, 463, 730
AluBI AGCT 6 cut(s) 60, 347, 474, 559, 828, 859
AluI AGCT 6 cut(s) 60, 347, 474, 559, 828, 859
Alw21I GWGCWC 2 cut(s) 62, 861
Alw26I GTCTC 3 cut(s) 214, 302, 813
AlwI GGATC 4 cut(s) 187, 259, 272, 334
AlwNI CAGNNNCTG 1 cut(s) 454
Ama87I CYCGRG 1 cut(s) 217
AoxI GGCC 8 cut(s) 44, 67, 606, 734, 747, 758, 781, 792
ApaI GGGCCC 1 cut(s) 48
ApeKI GCWGC 3 cut(s) 327, 566, 932
ApoI RAATTY 1 cut(s) 902
ArsI GACNNNNNNTTYG 2 cut(s) 639, 671
Asp700I GAANNNNTTC 1 cut(s) 365
Asp718I GGTACC 1 cut(s) 628
AspLEI GCGC 1 cut(s) 540
AspS9I GGNCC 9 cut(s) 44, 45, 67, 457, 607, 734, 747, 782, 831
AsuC2I CCSGG 2 cut(s) 444, 1028
AsuHPI GGTGA 2 cut(s) 361, 607
AvaI CYCGRG 1 cut(s) 217
AvaII GGWCC 2 cut(s) 457, 831
BaeGI GKGCMC 2 cut(s) 48, 444
BamHI GGATCC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 628, 848
BanII GRGCYC 3 cut(s) 48, 62, 861
Bbv12I GWGCWC 2 cut(s) 62, 861
BbvI GCAGC 3 cut(s) 314, 578, 919
BccI CCATC 3 cut(s) 34, 82, 626
BceAI ACGGC 3 cut(s) 54, 493, 862
BciT130I CCWGG 5 cut(s) 261, 317, 455, 465, 732
BciVI GTATCC 1 cut(s) 206
BcnI CCSGG 2 cut(s) 444, 1028
BcoDI GTCTC 3 cut(s) 214, 302, 813
BfuI GTATCC 1 cut(s) 206
BglI GCCNNNNNGGC 1 cut(s) 605
BisI GCNGC 4 cut(s) 185, 328, 567, 933
BlsI GCNGC 4 cut(s) 186, 329, 568, 934
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 7 cut(s) 261, 317, 444, 455, 465, 732, 1028
Bme18I GGWCC 2 cut(s) 457, 831
BmeT110I CYCGRG 1 cut(s) 217
BmgBI CACGTC 1 cut(s) 799
BmgT120I GGNCC 9 cut(s) 44, 45, 67, 457, 607, 734, 747, 782, 831
BmrFI CCNGG 7 cut(s) 261, 317, 444, 455, 465, 732, 1028
BmrI ACTGGG 2 cut(s) 847, 1024
BmsI GCATC 3 cut(s) 265, 792, 856
BmuI ACTGGG 2 cut(s) 847, 1024
BpmI CTGGAG 1 cut(s) 454
BpuEI CTTGAG 2 cut(s) 148, 875
BpuMI CCSGG 2 cut(s) 444, 1028
BsaHI GRCGYC 1 cut(s) 716
BsaI GGTCTC 2 cut(s) 214, 302
BsaJI CCNNGG 7 cut(s) 175, 260, 622, 730, 731, 778, 1026
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 6 cut(s) 168, 378, 624, 743, 756, 790
Bse118I RCCGGY 1 cut(s) 709
Bse1I ACTGG 4 cut(s) 413, 842, 933, 1019
Bse3DI GCAATG 1 cut(s) 977
BseBI CCWGG 5 cut(s) 261, 317, 455, 465, 732
BseDI CCNNGG 7 cut(s) 175, 260, 622, 730, 731, 778, 1026
BseGI GGATG 3 cut(s) 74, 256, 618
BseLI CCNNNNNNNGG 6 cut(s) 168, 378, 624, 743, 756, 790
BseMI GCAATG 1 cut(s) 977
BseNI ACTGG 4 cut(s) 413, 842, 933, 1019
BseRI GAGGAG 1 cut(s) 151
BseSI GKGCMC 2 cut(s) 48, 444
BseXI GCAGC 3 cut(s) 314, 578, 919
BseYI CCCAGC 2 cut(s) 555, 917
BsgI GTGCAG 1 cut(s) 775
Bsh1236I CGCG 2 cut(s) 396, 538
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 8 cut(s) 46, 69, 608, 736, 749, 760, 783, 794
BshNI GGYRCC 2 cut(s) 628, 848
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 2 cut(s) 62, 861
BsiHKCI CYCGRG 1 cut(s) 217
BsiSI CCGG 4 cut(s) 444, 570, 710, 1028
BslFI GGGAC 2 cut(s) 443, 1031
BslI CCNNNNNNNGG 6 cut(s) 168, 378, 624, 743, 756, 790
BsmAI GTCTC 3 cut(s) 214, 302, 813
BsmFI GGGAC 2 cut(s) 443, 1031
BsmI GAATGC 1 cut(s) 975
BsnI GGCC 8 cut(s) 46, 69, 608, 736, 749, 760, 783, 794
Bso31I GGTCTC 2 cut(s) 214, 302
BsoBI CYCGRG 1 cut(s) 217
Bsp120I GGGCCC 1 cut(s) 44
Bsp1286I GDGCHC 4 cut(s) 48, 62, 444, 861
Bsp143I GATC 5 cut(s) 111, 179, 264, 339, 897
BspANI GGCC 8 cut(s) 46, 69, 608, 736, 749, 760, 783, 794
BspFNI CGCG 2 cut(s) 396, 538
BspHI TCATGA 1 cut(s) 979
BspPI GGATC 4 cut(s) 187, 259, 272, 334
BspT107I GGYRCC 2 cut(s) 628, 848
BspTNI GGTCTC 2 cut(s) 214, 302
BsrBI CCGCTC 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 977
BsrFI RCCGGY 1 cut(s) 709
BsrI ACTGG 4 cut(s) 413, 842, 933, 1019
BssAI RCCGGY 1 cut(s) 709
BssECI CCNNGG 7 cut(s) 175, 260, 622, 730, 731, 778, 1026
BssMI GATC 5 cut(s) 111, 179, 264, 339, 897
BssNI GRCGYC 1 cut(s) 716
BssT1I CCWWGG 3 cut(s) 175, 622, 778
Bst2UI CCWGG 5 cut(s) 261, 317, 455, 465, 732
Bst4CI ACNGT 3 cut(s) 127, 404, 526
BstACI GRCGYC 1 cut(s) 716
BstAPI GCANNNNNTGC 1 cut(s) 762
BstC8I GCNNGC 3 cut(s) 758, 767, 955
BstF5I GGATG 3 cut(s) 74, 256, 618
BstFNI CGCG 2 cut(s) 396, 538
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 5 cut(s) 114, 182, 267, 342, 900
BstMAI GTCTC 3 cut(s) 214, 302, 813
BstMBI GATC 5 cut(s) 111, 179, 264, 339, 897
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 6 cut(s) 66, 605, 762, 766, 875, 1028
BstNI CCWGG 5 cut(s) 261, 317, 455, 465, 732
BstSCI CCNGG 7 cut(s) 259, 315, 442, 453, 463, 730, 1026
BstSLI GKGCMC 2 cut(s) 48, 444
BstUI CGCG 2 cut(s) 396, 538
BstV1I GCAGC 3 cut(s) 314, 578, 919
BstX2I RGATCY 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 33
BstYI RGATCY 1 cut(s) 264
BsuI GTATCC 1 cut(s) 206
BsuRI GGCC 8 cut(s) 46, 69, 608, 736, 749, 760, 783, 794
BtgZI GCGATG 1 cut(s) 879
BtrI CACGTC 1 cut(s) 799
BtsCI GGATG 3 cut(s) 74, 256, 618
BtsIMutI CAGTG 3 cut(s) 522, 835, 1048
Cac8I GCNNGC 3 cut(s) 758, 767, 955
CaiI CAGNNNCTG 1 cut(s) 454
CciI TCATGA 1 cut(s) 979
CfoI GCGC 1 cut(s) 540
Cfr10I RCCGGY 1 cut(s) 709
Cfr13I GGNCC 9 cut(s) 44, 45, 67, 457, 607, 734, 747, 782, 831
CseI GACGC 4 cut(s) 572, 705, 884, 1028
CsiI ACCWGGT 2 cut(s) 315, 453
Csp6I GTAC 4 cut(s) 433, 451, 629, 679
CviAII CATG 4 cut(s) 269, 872, 958, 980
CviQI GTAC 4 cut(s) 433, 451, 629, 679
DpnI GATC 5 cut(s) 113, 181, 266, 341, 899
DpnII GATC 5 cut(s) 111, 179, 264, 339, 897
EaeI YGGCCR 1 cut(s) 792
EciI GGCGGA 1 cut(s) 495
Ecl136II GAGCTC 2 cut(s) 60, 859
Eco130I CCWWGG 3 cut(s) 175, 622, 778
Eco147I AGGCCT 1 cut(s) 760
Eco24I GRGCYC 3 cut(s) 48, 62, 861
Eco31I GGTCTC 2 cut(s) 214, 302
Eco47I GGWCC 2 cut(s) 457, 831
Eco53kI GAGCTC 2 cut(s) 60, 859
Eco88I CYCGRG 1 cut(s) 217
EcoICRI GAGCTC 2 cut(s) 60, 859
EcoO109I RGGNCCY 1 cut(s) 44
EcoRII CCWGG 5 cut(s) 259, 315, 453, 463, 730
EcoT14I CCWWGG 3 cut(s) 175, 622, 778
EcoT22I ATGCAT 1 cut(s) 977
EcoT38I GRGCYC 3 cut(s) 48, 62, 861
ErhI CCWWGG 3 cut(s) 175, 622, 778
FaeI CATG 4 cut(s) 272, 875, 961, 983
FaiI YATR 6 cut(s) 270, 338, 775, 873, 959, 981
FaqI GGGAC 2 cut(s) 443, 1031
FatI CATG 4 cut(s) 268, 871, 957, 979
FauI CCCGC 3 cut(s) 401, 696, 737
Fnu4HI GCNGC 4 cut(s) 185, 328, 567, 933
FokI GGATG 3 cut(s) 61, 243, 605
FriOI GRGCYC 3 cut(s) 48, 62, 861
Fsp4HI GCNGC 4 cut(s) 185, 328, 567, 933
GlaI GCGC 1 cut(s) 539
GluI GCNGC 4 cut(s) 185, 328, 567, 933
GsaI CCCAGC 2 cut(s) 559, 921
GsuI CTGGAG 1 cut(s) 454
HaeIII GGCC 8 cut(s) 46, 69, 608, 736, 749, 760, 783, 794
HapII CCGG 4 cut(s) 444, 570, 710, 1028
HgaI GACGC 4 cut(s) 572, 705, 884, 1028
HhaI GCGC 1 cut(s) 540
Hin1I GRCGYC 1 cut(s) 716
Hin1II CATG 4 cut(s) 272, 875, 961, 983
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HincII GTYRAC 2 cut(s) 135, 400
HindII GTYRAC 2 cut(s) 135, 400
HinfI GANTC 5 cut(s) 20, 87, 483, 573, 648
HpaI GTTAAC 1 cut(s) 135
HpaII CCGG 4 cut(s) 444, 570, 710, 1028
HphI GGTGA 2 cut(s) 361, 607
Hpy166II GTNNAC 2 cut(s) 135, 400
Hpy188I TCNGA 4 cut(s) 111, 344, 389, 592
Hpy188III TCNNGA 2 cut(s) 17, 980
Hpy8I GTNNAC 2 cut(s) 135, 400
Hpy99I CGWCG 2 cut(s) 566, 800
HpyAV CCTTC 4 cut(s) 156, 317, 560, 610
HpyCH4III ACNGT 3 cut(s) 127, 404, 526
HpyCH4IV ACGT 2 cut(s) 636, 798
HpyCH4V TGCA 7 cut(s) 123, 304, 756, 765, 805, 953, 975
HpyF10VI GCNNNNNNNGC 6 cut(s) 66, 605, 762, 766, 875, 1028
HpySE526I ACGT 2 cut(s) 636, 798
Hsp92I GRCGYC 1 cut(s) 716
Hsp92II CATG 4 cut(s) 272, 875, 961, 983
HspAI GCGC 1 cut(s) 538
KpnI GGTACC 1 cut(s) 632
KspAI GTTAAC 1 cut(s) 135
Kzo9I GATC 5 cut(s) 111, 179, 264, 339, 897
LmnI GCTCC 4 cut(s) 164, 177, 473, 825
Lsp1109I GCAGC 3 cut(s) 314, 578, 919
LweI GCATC 3 cut(s) 265, 792, 856
MabI ACCWGGT 2 cut(s) 315, 453
MaeII ACGT 2 cut(s) 636, 798
MaeIII GTNAC 4 cut(s) 10, 404, 637, 664
MalI GATC 5 cut(s) 113, 181, 266, 341, 899
MbiI CCGCTC 1 cut(s) 744
MboI GATC 5 cut(s) 111, 179, 264, 339, 897
MboII GAAGA 4 cut(s) 189, 300, 934, 1009
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 4 cut(s) 48, 62, 444, 861
MluCI AATT 3 cut(s) 4, 105, 902
MlyI GAGTC 2 cut(s) 477, 642
MnlI CCTC 8 cut(s) 88, 172, 431, 521, 529, 542, 551, 557
Mph1103I ATGCAT 1 cut(s) 977
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 4 cut(s) 444, 570, 710, 1028
MspR9I CCNGG 7 cut(s) 261, 317, 444, 455, 465, 732, 1028
Mva1269I GAATGC 1 cut(s) 975
MvaI CCWGG 5 cut(s) 261, 317, 455, 465, 732
MvnI CGCG 2 cut(s) 396, 538
MwoI GCNNNNNNNGC 6 cut(s) 66, 605, 762, 766, 875, 1028
NciI CCSGG 2 cut(s) 444, 1028
NdeII GATC 5 cut(s) 111, 179, 264, 339, 897
NlaIII CATG 4 cut(s) 272, 875, 961, 983
NmeAIII GCCGAG 1 cut(s) 961
NmuCI GTSAC 2 cut(s) 10, 637
NsiI ATGCAT 1 cut(s) 977
PagI TCATGA 1 cut(s) 979
PasI CCCWGGG 1 cut(s) 731
PceI AGGCCT 1 cut(s) 760
PctI GAATGC 1 cut(s) 975
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 3 cut(s) 20, 87, 573
PkrI GCNGC 4 cut(s) 186, 329, 568, 934
PleI GAGTC 2 cut(s) 477, 642
PpsI GAGTC 2 cut(s) 477, 642
Psp124BI GAGCTC 2 cut(s) 62, 861
Psp6I CCWGG 5 cut(s) 259, 315, 453, 463, 730
PspFI CCCAGC 2 cut(s) 555, 917
PspGI CCWGG 5 cut(s) 259, 315, 453, 463, 730
PspOMI GGGCCC 1 cut(s) 44
PspPI GGNCC 9 cut(s) 44, 45, 67, 457, 607, 734, 747, 782, 831
PstNI CAGNNNCTG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 264
RsaI GTAC 4 cut(s) 434, 452, 630, 680
RsaNI GTAC 4 cut(s) 433, 451, 629, 679
SacI GAGCTC 2 cut(s) 62, 861
SaqAI TTAA 1 cut(s) 134
SatI GCNGC 4 cut(s) 185, 328, 567, 933
Sau3AI GATC 5 cut(s) 111, 179, 264, 339, 897
Sau96I GGNCC 9 cut(s) 44, 45, 67, 457, 607, 734, 747, 782, 831
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 2 cut(s) 477, 642
ScrFI CCNGG 7 cut(s) 261, 317, 444, 455, 465, 732, 1028
SduI GDGCHC 4 cut(s) 48, 62, 444, 861
SexAI ACCWGGT 2 cut(s) 315, 453
SfaNI GCATC 3 cut(s) 265, 792, 856
SinI GGWCC 2 cut(s) 457, 831
SmlI CTYRAG 2 cut(s) 163, 854
SmoI CTYRAG 2 cut(s) 163, 854
Sse9I AATT 3 cut(s) 4, 105, 902
SseBI AGGCCT 1 cut(s) 760
SspI AATATT 1 cut(s) 308
SstI GAGCTC 2 cut(s) 62, 861
StuI AGGCCT 1 cut(s) 760
StyD4I CCNGG 7 cut(s) 259, 315, 442, 453, 463, 730, 1026
StyI CCWWGG 3 cut(s) 175, 622, 778
TaaI ACNGT 3 cut(s) 127, 404, 526
TaiI ACGT 2 cut(s) 639, 801
TaqI TCGA 4 cut(s) 56, 80, 276, 561
TaqII GACCGA 2 cut(s) 238, 924
TasI AATT 3 cut(s) 4, 105, 902
TatI WGTACW 1 cut(s) 432
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 3 cut(s) 20, 87, 573
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TscAI CASTG 2 cut(s) 529, 842
TseFI GTSAC 2 cut(s) 10, 637
TseI GCWGC 3 cut(s) 327, 566, 932
Tsp45I GTSAC 2 cut(s) 10, 637
TspDTI ATGAA 3 cut(s) 17, 968, 1010
TspRI CASTG 2 cut(s) 529, 842
VpaK11BI GGWCC 2 cut(s) 457, 831
XapI RAATTY 1 cut(s) 902
XmnI GAANNNNTTC 1 cut(s) 365
ZrmI AGTACT 1 cut(s) 434
Zsp2I ATGCAT 1 cut(s) 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.