MD08G1003100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011290
Physical Location & Seq
Forward (+)
342577 .. 346324
3748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1003100.v1.1.491

Sequence Viewer

Length: 1356 bp
ATGGTGAAGTGGACAACCCTCCTCCCTGAAAACAGGATGACCGTCTATTTCTCCAGATGGTTTTCCGATCTCGATTGGCGCCGGCTGCTCTTACTCATCCCTCCCCTATCTCTCATACTCTTCCTCTCACTCTCCTCCTTCTCTTCTTCTTCTTCTTTTTTCTCTCCCACCAACATCCCTTTTGCTCCCATCCGATCCATCTTTCGCGGCGGCCGCGCCTTCCAGTTCCAGGTGCCGTCCAACAACTTCAATTCAACGCCAATTGCTTTGCCTCCAAACCAAAATCAAGATCGGACCCAGTCGTTGATCAAGAAGAAGGAGGATGAATTGGATCGCTCCAGAATCGCCGTCTGCTTGGTAGGTGGGGCCCGGAGGTTCGAGCTCACGGGGCCGTCCATCCTCGACAAGATTCTCAACAAATATTCCAACTCCGATCTTTTCTTGCACAGTCCTGTGGACCCCAACGCCTTCAAGTTCTCGCTCCTCAAATCGGCGCCAAGGATCGCCGCCGTCAAAATCTTCCACCCCAAACCAATGCCCGAGACTGAGTTCCAGCTCCGAGTCCTAACCGCCCAGAACTCGCCCAATGGCATCCAGGGTCTGTTGCAATATTTCAACCTGGTGGAAGGTTGCCTAACTATGATCCAAGCTTACCAAAAACAGAACAACTTCACCTACGACTGGATTGTCCGAACCCGAGTTGACGGCTACTGGAACGCCCCCCTCCACCCCAAATACTTCGTGCCCGGACGCTACCTGGTCCCACCAGGCTCCAGCTATGGCGGACTCAACGACCGCTTTGGAGTTGGCGACCTCAACACCTCCACTGTCGCCCTCTCCCGCCTCTCCCTCCTTCCCCAGCTCGACGCAGCCGGATTCCACCAACTTAACTCCGAAACCGCCTTCAAGGCCCAACTCACCACCCAAGGGGTACCTTACGTCACTAAACGACTCCCCTTTTGTATCGTAACAGACCGCCAGTACGAGTTCCCGCCACACCGCTTTGGAGTGCCGGTGGCGTCCTTGTCAAGCCCAGGGCCATTGAGCGGGGCCAAGTGCAGGCCTTGCAAGCCTGTGTGCCGAGGCCCGTGCGTGGCAGACGTGATGTTGAGCCTCTACAAAGGGTGGAGCTGGACCCACTGGGCCAACGGCACCCTTGAGCTCTGTGATGCCCATGAAGCTTGGGAAAAGAATTGGGAGAGCAAATTTGACCGAGTTGCTGGGAAGAAACTCGCTGCCGAGCGAAAGCGAGTTGCAGGCATAACGACAGAGCAATGCATTCATGATTTCAACCAACTGAAGAACCGAACTGCCAACTGGGACGCACCGCCGGCGGAACAGATTTGTAGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

452

Amino Acids

50.9

Weight (kDa)

9.44

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7796 PF25072 111 - 450 4.2e-187 Domain of unknown function (DUF7796)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
Acc65I GGTACC 1 cut(s) 931
AccB1I GGYRCC 5 cut(s) 78, 232, 493, 931, 1151
AccBSI CCGCTC 1 cut(s) 1047
AccII CGCG 2 cut(s) 207, 216
AclWI GGATC 4 cut(s) 189, 339, 509, 637
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 1205
AcuI CTGAAG 1 cut(s) 1319
AcyI GRCGYC 3 cut(s) 79, 494, 1019
AfaI GTAC 2 cut(s) 933, 983
AfiI CCNNNNNNNGG 7 cut(s) 229, 490, 681, 927, 1046, 1059, 1093
AgsI TTSAA 6 cut(s) 250, 255, 472, 616, 907, 1291
AjiI CACGTC 1 cut(s) 1102
AjnI CCWGG 6 cut(s) 228, 594, 618, 756, 766, 1033
AluBI AGCT 8 cut(s) 382, 556, 650, 777, 862, 1131, 1162, 1181
AluI AGCT 8 cut(s) 382, 556, 650, 777, 862, 1131, 1162, 1181
Alw21I GWGCWC 2 cut(s) 384, 1164
Alw26I GTCTC 1 cut(s) 536
AlwI GGATC 4 cut(s) 189, 339, 509, 637
AlwNI CAGNNNCTG 1 cut(s) 601
Ama87I CYCGRG 2 cut(s) 539, 696
AoxI GGCC 9 cut(s) 211, 366, 389, 909, 1037, 1050, 1061, 1084, 1143
ApaI GGGCCC 1 cut(s) 370
ApeKI GCWGC 3 cut(s) 85, 869, 1235
ApoI RAATTY 1 cut(s) 1205
ArsI GACNNNNNNTTYG 2 cut(s) 942, 974
Asp700I GAANNNNTTC 1 cut(s) 668
Asp718I GGTACC 1 cut(s) 931
AspLEI GCGC 3 cut(s) 81, 218, 496
AsuC2I CCSGG 2 cut(s) 370, 747
AsuHPI GGTGA 3 cut(s) 16, 664, 910
AvaI CYCGRG 2 cut(s) 539, 696
AvaII GGWCC 4 cut(s) 294, 457, 760, 1134
BaeGI GKGCMC 2 cut(s) 370, 747
BanI GGYRCC 5 cut(s) 78, 232, 493, 931, 1151
BanII GRGCYC 3 cut(s) 370, 384, 1164
Bbv12I GWGCWC 2 cut(s) 384, 1164
BbvI GCAGC 3 cut(s) 72, 881, 1222
BccI CCATC 4 cut(s) 51, 197, 206, 404
BceAI ACGGC 6 cut(s) 220, 332, 376, 494, 721, 1165
BcgI CGANNNNNNTGC 2 cut(s) 1071, 1105
BciT130I CCWGG 6 cut(s) 230, 596, 620, 758, 768, 1035
BclI TGATCA 1 cut(s) 306
BcnI CCSGG 2 cut(s) 370, 747
BcoDI GTCTC 1 cut(s) 536
BfoI RGCGCY 2 cut(s) 82, 497
BglI GCCNNNNNGGC 1 cut(s) 908
BisI GCNGC 7 cut(s) 86, 208, 211, 214, 507, 870, 1236
BlsI GCNGC 7 cut(s) 87, 209, 212, 215, 508, 871, 1237
Bme1390I CCNGG 8 cut(s) 230, 370, 596, 620, 747, 758, 768, 1035
Bme18I GGWCC 4 cut(s) 294, 457, 760, 1134
BmeT110I CYCGRG 2 cut(s) 539, 696
BmgBI CACGTC 1 cut(s) 1102
BmrFI CCNGG 8 cut(s) 230, 370, 596, 620, 747, 758, 768, 1035
BmrI ACTGGG 3 cut(s) 292, 1150, 1327
BmsI GCATC 2 cut(s) 600, 1159
BmuI ACTGGG 3 cut(s) 292, 1150, 1327
BpmI CTGGAG 3 cut(s) 37, 322, 757
BpuEI CTTGAG 1 cut(s) 1178
BpuMI CCSGG 2 cut(s) 370, 747
BsaHI GRCGYC 3 cut(s) 79, 494, 1019
BsaJI CCNNGG 6 cut(s) 497, 595, 925, 1033, 1034, 1081
BsaXI ACNNNNNCTCC 2 cut(s) 999, 1029
Bsc4I CCNNNNNNNGG 7 cut(s) 229, 490, 681, 927, 1046, 1059, 1093
Bse118I RCCGGY 3 cut(s) 81, 1012, 1330
Bse1I ACTGG 7 cut(s) 223, 298, 686, 716, 979, 1145, 1322
Bse3DI GCAATG 1 cut(s) 1280
BseBI CCWGG 6 cut(s) 230, 596, 620, 758, 768, 1035
BseDI CCNNGG 6 cut(s) 497, 595, 925, 1033, 1034, 1081
BseGI GGATG 7 cut(s) 42, 96, 174, 189, 328, 396, 591
BseLI CCNNNNNNNGG 7 cut(s) 229, 490, 681, 927, 1046, 1059, 1093
BseMI GCAATG 1 cut(s) 1280
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 7 cut(s) 223, 298, 686, 716, 979, 1145, 1322
BseRI GAGGAG 3 cut(s) 11, 124, 473
BseSI GKGCMC 2 cut(s) 370, 747
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 3 cut(s) 72, 881, 1222
BseYI CCCAGC 2 cut(s) 858, 1220
BsgI GTGCAG 1 cut(s) 1078
Bsh1236I CGCG 2 cut(s) 207, 216
Bsh1285I CGRYCG 2 cut(s) 214, 796
BshFI GGCC 9 cut(s) 213, 368, 391, 911, 1039, 1052, 1063, 1086, 1145
BshNI GGYRCC 5 cut(s) 78, 232, 493, 931, 1151
BsiEI CGRYCG 2 cut(s) 214, 796
BsiHKAI GWGCWC 2 cut(s) 384, 1164
BsiHKCI CYCGRG 2 cut(s) 539, 696
BsiSI CCGG 6 cut(s) 82, 370, 747, 873, 1013, 1331
BslFI GGGAC 2 cut(s) 746, 1334
BslI CCNNNNNNNGG 7 cut(s) 229, 490, 681, 927, 1046, 1059, 1093
BsmAI GTCTC 1 cut(s) 536
BsmFI GGGAC 2 cut(s) 746, 1334
BsmI GAATGC 1 cut(s) 1278
BsnI GGCC 9 cut(s) 213, 368, 391, 911, 1039, 1052, 1063, 1086, 1145
BsoBI CYCGRG 2 cut(s) 539, 696
Bsp120I GGGCCC 1 cut(s) 366
Bsp1286I GDGCHC 4 cut(s) 370, 384, 747, 1164
Bsp143I GATC 8 cut(s) 67, 194, 289, 306, 331, 433, 501, 642
BspANI GGCC 9 cut(s) 213, 368, 391, 911, 1039, 1052, 1063, 1086, 1145
BspCNI CTCAG 1 cut(s) 538
BspFNI CGCG 2 cut(s) 207, 216
BspHI TCATGA 1 cut(s) 1282
BspPI GGATC 4 cut(s) 189, 339, 509, 637
BspT107I GGYRCC 5 cut(s) 78, 232, 493, 931, 1151
BsrBI CCGCTC 1 cut(s) 1047
BsrDI GCAATG 1 cut(s) 1280
BsrFI RCCGGY 3 cut(s) 81, 1012, 1330
BsrI ACTGG 7 cut(s) 223, 298, 686, 716, 979, 1145, 1322
BssAI RCCGGY 3 cut(s) 81, 1012, 1330
BssECI CCNNGG 6 cut(s) 497, 595, 925, 1033, 1034, 1081
BssMI GATC 8 cut(s) 67, 194, 289, 306, 331, 433, 501, 642
BssNI GRCGYC 3 cut(s) 79, 494, 1019
BssT1I CCWWGG 2 cut(s) 497, 925
Bst2UI CCWGG 6 cut(s) 230, 596, 620, 758, 768, 1035
Bst4CI ACNGT 3 cut(s) 43, 449, 829
Bst6I CTCTTC 2 cut(s) 125, 148
BstACI GRCGYC 3 cut(s) 79, 494, 1019
BstAPI GCANNNNNTGC 1 cut(s) 1065
BstC8I GCNNGC 5 cut(s) 83, 1061, 1070, 1258, 1332
BstDEI CTNAG 1 cut(s) 546
BstF5I GGATG 7 cut(s) 42, 96, 174, 189, 328, 396, 591
BstFNI CGCG 2 cut(s) 207, 216
BstH2I RGCGCY 2 cut(s) 82, 497
BstHHI GCGC 3 cut(s) 81, 218, 496
BstKTI GATC 8 cut(s) 70, 197, 292, 309, 334, 436, 504, 645
BstMAI GTCTC 1 cut(s) 536
BstMBI GATC 8 cut(s) 67, 194, 289, 306, 331, 433, 501, 642
BstMCI CGRYCG 2 cut(s) 214, 796
BstMWI GCNNNNNNNGC 8 cut(s) 85, 213, 388, 908, 1065, 1069, 1178, 1331
BstNI CCWGG 6 cut(s) 230, 596, 620, 758, 768, 1035
BstSCI CCNGG 8 cut(s) 228, 368, 594, 618, 745, 756, 766, 1033
BstSLI GKGCMC 2 cut(s) 370, 747
BstUI CGCG 2 cut(s) 207, 216
BstV1I GCAGC 3 cut(s) 72, 881, 1222
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 9 cut(s) 213, 368, 391, 911, 1039, 1052, 1063, 1086, 1145
BtrI CACGTC 1 cut(s) 1102
BtsCI GGATG 7 cut(s) 42, 96, 174, 189, 328, 396, 591
BtsIMutI CAGTG 3 cut(s) 825, 1138, 1351
Cac8I GCNNGC 5 cut(s) 83, 1061, 1070, 1258, 1332
CaiI CAGNNNCTG 1 cut(s) 601
CciI TCATGA 1 cut(s) 1282
CciNI GCGGCCGC 1 cut(s) 211
CfoI GCGC 3 cut(s) 81, 218, 496
Cfr10I RCCGGY 3 cut(s) 81, 1012, 1330
CseI GACGC 4 cut(s) 759, 875, 1008, 1331
CsiI ACCWGGT 2 cut(s) 618, 756
Csp6I GTAC 2 cut(s) 932, 982
CviAII CATG 2 cut(s) 1175, 1283
CviQI GTAC 2 cut(s) 932, 982
DdeI CTNAG 1 cut(s) 546
DinI GGCGCC 2 cut(s) 80, 495
DpnI GATC 8 cut(s) 69, 196, 291, 308, 333, 435, 503, 644
DpnII GATC 8 cut(s) 67, 194, 289, 306, 331, 433, 501, 642
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
Eam1104I CTCTTC 2 cut(s) 125, 148
EarI CTCTTC 2 cut(s) 125, 148
EciI GGCGGA 2 cut(s) 798, 1349
Ecl136II GAGCTC 2 cut(s) 382, 1162
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 497, 925
Eco147I AGGCCT 1 cut(s) 1063
Eco24I GRGCYC 3 cut(s) 370, 384, 1164
Eco47I GGWCC 4 cut(s) 294, 457, 760, 1134
Eco52I CGGCCG 1 cut(s) 211
Eco53kI GAGCTC 2 cut(s) 382, 1162
Eco57I CTGAAG 1 cut(s) 1319
Eco88I CYCGRG 2 cut(s) 539, 696
EcoICRI GAGCTC 2 cut(s) 382, 1162
EcoO109I RGGNCCY 1 cut(s) 366
EcoRII CCWGG 6 cut(s) 228, 594, 618, 756, 766, 1033
EcoT14I CCWWGG 2 cut(s) 497, 925
EcoT22I ATGCAT 1 cut(s) 1280
EcoT38I GRGCYC 3 cut(s) 370, 384, 1164
EgeI GGCGCC 2 cut(s) 80, 495
EheI GGCGCC 2 cut(s) 80, 495
ErhI CCWWGG 2 cut(s) 497, 925
FaeI CATG 2 cut(s) 1178, 1286
FaiI YATR 6 cut(s) 116, 641, 780, 1176, 1262, 1284
FaqI GGGAC 2 cut(s) 746, 1334
FatI CATG 2 cut(s) 1174, 1282
FauI CCCGC 3 cut(s) 848, 999, 1040
FbaI TGATCA 1 cut(s) 306
Fnu4HI GCNGC 7 cut(s) 86, 208, 211, 214, 507, 870, 1236
FokI GGATG 7 cut(s) 49, 83, 161, 176, 335, 383, 578
FriOI GRGCYC 3 cut(s) 370, 384, 1164
Fsp4HI GCNGC 7 cut(s) 86, 208, 211, 214, 507, 870, 1236
GlaI GCGC 3 cut(s) 80, 217, 495
GluI GCNGC 7 cut(s) 86, 208, 211, 214, 507, 870, 1236
GsaI CCCAGC 2 cut(s) 862, 1224
GsuI CTGGAG 3 cut(s) 37, 322, 757
HaeII RGCGCY 2 cut(s) 82, 497
HaeIII GGCC 9 cut(s) 213, 368, 391, 911, 1039, 1052, 1063, 1086, 1145
HapII CCGG 6 cut(s) 82, 370, 747, 873, 1013, 1331
HgaI GACGC 4 cut(s) 759, 875, 1008, 1331
HhaI GCGC 3 cut(s) 81, 218, 496
Hin1I GRCGYC 3 cut(s) 79, 494, 1019
Hin1II CATG 2 cut(s) 1178, 1286
Hin6I GCGC 3 cut(s) 79, 216, 494
HinP1I GCGC 3 cut(s) 79, 216, 494
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HindIII AAGCTT 2 cut(s) 648, 1179
HinfI GANTC 6 cut(s) 342, 409, 561, 786, 876, 951
HpaII CCGG 6 cut(s) 82, 370, 747, 873, 1013, 1331
HphI GGTGA 3 cut(s) 16, 664, 910
Hpy166II GTNNAC 3 cut(s) 12, 457, 703
Hpy188I TCNGA 7 cut(s) 67, 194, 294, 433, 560, 692, 895
Hpy188III TCNNGA 6 cut(s) 54, 71, 287, 310, 339, 1283
Hpy8I GTNNAC 3 cut(s) 12, 457, 703
Hpy99I CGWCG 1 cut(s) 869
HpyAV CCTTC 7 cut(s) 148, 229, 310, 478, 620, 863, 913
HpyCH4III ACNGT 3 cut(s) 43, 449, 829
HpyCH4IV ACGT 2 cut(s) 939, 1101
HpyCH4V TGCA 6 cut(s) 445, 607, 1059, 1068, 1256, 1278
HpyF10VI GCNNNNNNNGC 8 cut(s) 85, 213, 388, 908, 1065, 1069, 1178, 1331
HpyF3I CTNAG 1 cut(s) 546
HpySE526I ACGT 2 cut(s) 939, 1101
Hsp92I GRCGYC 3 cut(s) 79, 494, 1019
Hsp92II CATG 2 cut(s) 1178, 1286
HspAI GCGC 3 cut(s) 79, 216, 494
KasI GGCGCC 2 cut(s) 78, 493
KpnI GGTACC 1 cut(s) 935
KroI GCCGGC 2 cut(s) 81, 1330
KroNI GCCGGC 2 cut(s) 83, 1332
Ksp22I TGATCA 1 cut(s) 306
Kzo9I GATC 8 cut(s) 67, 194, 289, 306, 331, 433, 501, 642
LmnI GCTCC 6 cut(s) 190, 341, 486, 561, 776, 1128
Lsp1109I GCAGC 3 cut(s) 72, 881, 1222
LweI GCATC 2 cut(s) 600, 1159
MabI ACCWGGT 2 cut(s) 618, 756
MaeII ACGT 2 cut(s) 939, 1101
MaeIII GTNAC 2 cut(s) 940, 967
MalI GATC 8 cut(s) 69, 196, 291, 308, 333, 435, 503, 644
MbiI CCGCTC 1 cut(s) 1047
MboI GATC 8 cut(s) 67, 194, 289, 306, 331, 433, 501, 642
MboII GAAGA 9 cut(s) 112, 135, 138, 141, 144, 325, 511, 1237, 1312
MfeI CAATTG 1 cut(s) 261
MhlI GDGCHC 4 cut(s) 370, 384, 747, 1164
MluCI AATT 5 cut(s) 250, 261, 326, 1192, 1205
Mly113I GGCGCC 2 cut(s) 79, 494
MlyI GAGTC 3 cut(s) 570, 780, 945
MmeI TCCRAC 2 cut(s) 264, 450
Mph1103I ATGCAT 1 cut(s) 1280
MreI CGCCGGCG 1 cut(s) 1330
MroNI GCCGGC 2 cut(s) 81, 1330
MroXI GAANNNNTTC 1 cut(s) 668
MseI TTAA 1 cut(s) 888
MspI CCGG 6 cut(s) 82, 370, 747, 873, 1013, 1331
MspR9I CCNGG 8 cut(s) 230, 370, 596, 620, 747, 758, 768, 1035
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 1278
MvaI CCWGG 6 cut(s) 230, 596, 620, 758, 768, 1035
MvnI CGCG 2 cut(s) 207, 216
MwoI GCNNNNNNNGC 8 cut(s) 85, 213, 388, 908, 1065, 1069, 1178, 1331
NaeI GCCGGC 2 cut(s) 83, 1332
NarI GGCGCC 2 cut(s) 79, 494
NciI CCSGG 2 cut(s) 370, 747
NdeII GATC 8 cut(s) 67, 194, 289, 306, 331, 433, 501, 642
NgoMIV GCCGGC 2 cut(s) 81, 1330
NlaIII CATG 2 cut(s) 1178, 1286
NmeAIII GCCGAG 2 cut(s) 1106, 1264
NmuCI GTSAC 1 cut(s) 940
NotI GCGGCCGC 1 cut(s) 211
NsiI ATGCAT 1 cut(s) 1280
PagI TCATGA 1 cut(s) 1282
PasI CCCWGGG 1 cut(s) 1034
PceI AGGCCT 1 cut(s) 1063
PctI GAATGC 1 cut(s) 1278
PdiI GCCGGC 2 cut(s) 83, 1332
PdmI GAANNNNTTC 1 cut(s) 668
PfeI GAWTC 3 cut(s) 342, 409, 876
PflFI GACNNNGTC 1 cut(s) 298
PkrI GCNGC 7 cut(s) 87, 209, 212, 215, 508, 871, 1237
PleI GAGTC 3 cut(s) 569, 780, 945
PluTI GGCGCC 2 cut(s) 82, 497
PpsI GAGTC 3 cut(s) 569, 780, 945
Psp124BI GAGCTC 2 cut(s) 384, 1164
Psp6I CCWGG 6 cut(s) 228, 594, 618, 756, 766, 1033
PspFI CCCAGC 2 cut(s) 858, 1220
PspGI CCWGG 6 cut(s) 228, 594, 618, 756, 766, 1033
PspOMI GGGCCC 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 601
PsyI GACNNNGTC 1 cut(s) 298
RsaI GTAC 2 cut(s) 933, 983
RsaNI GTAC 2 cut(s) 932, 982
SacI GAGCTC 2 cut(s) 384, 1164
SaqAI TTAA 1 cut(s) 888
SatI GCNGC 7 cut(s) 86, 208, 211, 214, 507, 870, 1236
Sau3AI GATC 8 cut(s) 67, 194, 289, 306, 331, 433, 501, 642
SchI GAGTC 3 cut(s) 570, 780, 945
ScrFI CCNGG 8 cut(s) 230, 370, 596, 620, 747, 758, 768, 1035
SduI GDGCHC 4 cut(s) 370, 384, 747, 1164
SexAI ACCWGGT 2 cut(s) 618, 756
SfaNI GCATC 2 cut(s) 600, 1159
SfoI GGCGCC 2 cut(s) 80, 495
SgrAI CRCCGGYG 1 cut(s) 1330
SinI GGWCC 4 cut(s) 294, 457, 760, 1134
SmlI CTYRAG 1 cut(s) 1157
SmoI CTYRAG 1 cut(s) 1157
Sse9I AATT 5 cut(s) 250, 261, 326, 1192, 1205
SseBI AGGCCT 1 cut(s) 1063
SspDI GGCGCC 2 cut(s) 78, 493
SspI AATATT 2 cut(s) 422, 611
SstI GAGCTC 2 cut(s) 384, 1164
StuI AGGCCT 1 cut(s) 1063
StyD4I CCNGG 8 cut(s) 228, 368, 594, 618, 745, 756, 766, 1033
StyI CCWWGG 2 cut(s) 497, 925
TaaI ACNGT 3 cut(s) 43, 449, 829
TaiI ACGT 2 cut(s) 942, 1104
TaqI TCGA 4 cut(s) 72, 378, 402, 864
TaqII GACCGA 1 cut(s) 1227
TasI AATT 5 cut(s) 250, 261, 326, 1192, 1205
TauI GCSGC 4 cut(s) 210, 213, 216, 509
TfiI GAWTC 3 cut(s) 342, 409, 876
Tru1I TTAA 1 cut(s) 888
Tru9I TTAA 1 cut(s) 888
TscAI CASTG 2 cut(s) 832, 1145
TseFI GTSAC 1 cut(s) 940
TseI GCWGC 3 cut(s) 85, 869, 1235
Tsp45I GTSAC 1 cut(s) 940
TspDTI ATGAA 3 cut(s) 339, 1191, 1271
TspRI CASTG 2 cut(s) 832, 1145
Tth111I GACNNNGTC 1 cut(s) 298
VpaK11BI GGWCC 4 cut(s) 294, 457, 760, 1134
XapI RAATTY 1 cut(s) 1205
XmnI GAANNNNTTC 1 cut(s) 668
Zsp2I ATGCAT 1 cut(s) 1280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.