Rorug06G0328200

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010031
Physical Location & Seq
Reverse (-)
48117845 .. 48119914
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0328200.1

Sequence Viewer

Length: 1215 bp
ATGGCCACTCTTGCTTCATCATCATCATCCTCTGTGCTCTTCTCCTCTCCACACTCCAACCTCACTCCACCTTCTCTCTCTCCTTCATGCCTCTCTCTCTCCTCTTCCTCCCACCTCTCCACCACTCTCTCCTCTTCCCTCTCACTTTCTCATTCTTTTGTCACATACCCTACAACTTCAAGGCGCTTCAGCCCTAGTTCCTTCAGTGTCAAGGCTATTGCTGCAGACAAGAAGAAAGTTCTGATCGTCAACACCAACAGTGGTGGTCATGCAGTCATTGGATTCTACTTTGCAAAAGAGCTTCTGGGCTCTGGCCATGAGGTCACCATTATGACTGTTGGTGAAGAAAGCTCAGACAAGATGAAGAAGACCCCATTCACCAGATTCTCGGAAATTGTAAGTGCTGGAGGGAAGACTGTGTGGGGAGAACCAGCAGAAATAGCTAAGGTTTTGGAAGGATCAGCATTTGATGTTGTGTTGGATAACAATGGCAAGAACTTGGACGCAGTGAAGCCTGTGGCTGATTGGGCAAAGAGTTCTGGTGCAAAGCAATTCCTATTTATCAGCAGTGCTGGGATTTATAAGCCAACCGAGGAGCCTCCTCATGTCGAAGGGGATGTCGTTAAAGCTGATGCTGGTCATGTTGCAGTAGAGAAGTACATTGCAGAAGTGTTTGGTAATTGGGCAAGTTTTCGTCCACAATACATGATAGGATCCGGCAACAACAAAGATTGCGAGGAGTGGTTCTTCGATCGAATTGTGAGGGACAGACCAGTTCCAATCCCTGGCTCCGGAATGCAACTCACAGTCATCTCCCATGTTAGGGACTTGTCCTCTATGCTTACTCTAGCCGTTGCGAAGCCAGATGCTGCATCTGGTAATATTTTCAACTGTGTAAGCGACCGTGCTGTGACTCTCGATGGATTGGCAAAACTCTGTGCTCAAGCTGCAGGACGCCCGGTGAACATAGTGCATTATGATCCAAAAGCTGCTGGGGTTGATGCAAAGAAAGCTTTTCCGTTCCGTAACATGCACTTCTATGCAGAACCAAGAGCTGCAAAGGATATCTTGGGATGGAAAAGTACCACAAACCTCACTGAAGACTTGAAAGAGAGATTTGAGGAGTACTTGAAGATTGGGAGAGACAAGAAAGCCATCAAATTTGATTTGGATGATAAGATACTGGAGTCCCTTAAAGTTCCAGTAGCAGTGTGA

Protein Analysis

404

Amino Acids

43.6

Weight (kDa)

8.18

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 88 - 298 2.4e-08 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
AccB7I CCANNNNNTGG 1 cut(s) 785
AccIII TCCGGA 1 cut(s) 791
AclWI GGATC 4 cut(s) 466, 708, 721, 974
AcoI YGGCCR 2 cut(s) 3, 313
AcsI RAATTY 1 cut(s) 1160
AcuI CTGAAG 3 cut(s) 172, 187, 1119
AcyI GRCGYC 1 cut(s) 955
AfaI GTAC 3 cut(s) 659, 1084, 1127
AfiI CCNNNNNNNGG 4 cut(s) 785, 791, 822, 823
AgsI TTSAA 4 cut(s) 180, 889, 1108, 1132
AjnI CCWGG 1 cut(s) 784
AluBI AGCT 8 cut(s) 301, 351, 443, 629, 947, 989, 1013, 1055
AluI AGCT 8 cut(s) 301, 351, 443, 629, 947, 989, 1013, 1055
Alw21I GWGCWC 2 cut(s) 39, 943
Alw26I GTCTC 1 cut(s) 1137
AlwI GGATC 4 cut(s) 466, 708, 721, 974
AlwNI CAGNNNCTG 1 cut(s) 869
Aor13HI TCCGGA 1 cut(s) 791
AoxI GGCC 2 cut(s) 3, 313
ApeKI GCWGC 5 cut(s) 221, 869, 947, 989, 1055
ApoI RAATTY 1 cut(s) 1160
ArsI GACNNNNNNTTYG 2 cut(s) 603, 635
AspLEI GCGC 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 959
AsuHPI GGTGA 4 cut(s) 316, 353, 370, 973
BaeI ACNNNNGTAYC 2 cut(s) 1172, 1205
BalI TGGCCA 2 cut(s) 5, 315
BamHI GGATCC 1 cut(s) 713
BanII GRGCYC 1 cut(s) 311
BbsI GAAGAC 3 cut(s) 374, 419, 1107
Bbv12I GWGCWC 2 cut(s) 39, 943
BbvI GCAGC 5 cut(s) 208, 856, 934, 976, 1042
BccI CCATC 3 cut(s) 914, 1068, 1163
BceAI ACGGC 1 cut(s) 836
BciT130I CCWGG 1 cut(s) 786
BcnI CCSGG 1 cut(s) 959
BcoDI GTCTC 1 cut(s) 1137
BfaI CTAG 2 cut(s) 195, 848
BfmI CTRYAG 2 cut(s) 222, 948
BfoI RGCGCY 1 cut(s) 187
BisI GCNGC 5 cut(s) 222, 870, 948, 990, 1056
BlsI GCNGC 5 cut(s) 223, 871, 949, 991, 1057
BmcAI AGTACT 1 cut(s) 1127
Bme1390I CCNGG 2 cut(s) 786, 959
BmiI GGNNCC 3 cut(s) 597, 715, 790
BmrFI CCNGG 2 cut(s) 786, 959
BmsI GCATC 4 cut(s) 622, 856, 881, 991
BpiI GAAGAC 3 cut(s) 374, 419, 1107
BpmI CTGGAG 2 cut(s) 426, 1205
Bpu10I CCTNAGC 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 927
BpuMI CCSGG 1 cut(s) 959
BsaHI GRCGYC 1 cut(s) 955
BsaJI CCNNGG 2 cut(s) 591, 784
BsaWI WCCGGW 1 cut(s) 791
Bsc4I CCNNNNNNNGG 4 cut(s) 785, 791, 822, 823
Bse1I ACTGG 3 cut(s) 773, 1188, 1202
Bse3DI GCAATG 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 791
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 591, 784
BseGI GGATG 4 cut(s) 26, 622, 1079, 1177
BseLI CCNNNNNNNGG 4 cut(s) 785, 791, 822, 823
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 3 cut(s) 773, 1188, 1202
BseRI GAGGAG 7 cut(s) 34, 91, 121, 591, 608, 752, 1136
BseXI GCAGC 5 cut(s) 208, 856, 934, 976, 1042
BseYI CCCAGC 2 cut(s) 572, 992
Bsh1285I CGRYCG 2 cut(s) 754, 904
BshFI GGCC 2 cut(s) 5, 315
BsiEI CGRYCG 2 cut(s) 754, 904
BsiHKAI GWGCWC 2 cut(s) 39, 943
BsiSI CCGG 3 cut(s) 717, 792, 959
BslFI GGGAC 3 cut(s) 779, 839, 1174
BslI CCNNNNNNNGG 4 cut(s) 785, 791, 822, 823
BsmAI GTCTC 1 cut(s) 1137
BsmFI GGGAC 3 cut(s) 779, 839, 1174
BsmI GAATGC 1 cut(s) 801
BsnI GGCC 2 cut(s) 5, 315
Bsp1286I GDGCHC 3 cut(s) 39, 311, 943
Bsp13I TCCGGA 1 cut(s) 791
Bsp143I GATC 5 cut(s) 243, 458, 713, 751, 979
BspANI GGCC 2 cut(s) 5, 315
BspCNI CTCAG 1 cut(s) 365
BspEI TCCGGA 1 cut(s) 791
BspLI GGNNCC 3 cut(s) 597, 715, 790
BspMAI CTGCAG 2 cut(s) 226, 952
BspPI GGATC 4 cut(s) 466, 708, 721, 974
BspQI GCTCTTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 660
BsrI ACTGG 3 cut(s) 773, 1188, 1202
BssECI CCNNGG 2 cut(s) 591, 784
BssMI GATC 5 cut(s) 243, 458, 713, 751, 979
BssNI GRCGYC 1 cut(s) 955
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 6 cut(s) 260, 337, 418, 808, 893, 905
Bst6I CTCTTC 3 cut(s) 44, 109, 139
BstACI GRCGYC 1 cut(s) 955
BstDEI CTNAG 2 cut(s) 352, 444
BstEII GGTNACC 1 cut(s) 322
BstF5I GGATG 4 cut(s) 26, 622, 1079, 1177
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 5 cut(s) 246, 461, 716, 754, 982
BstMAI GTCTC 1 cut(s) 1137
BstMBI GATC 5 cut(s) 243, 458, 713, 751, 979
BstMCI CGRYCG 2 cut(s) 754, 904
BstMWI GCNNNNNNNGC 6 cut(s) 11, 221, 440, 527, 947, 1010
BstNI CCWGG 1 cut(s) 786
BstNSI RCATGY 1 cut(s) 1033
BstPI GGTNACC 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 784, 957
BstSFI CTRYAG 2 cut(s) 222, 948
BstV1I GCAGC 5 cut(s) 208, 856, 934, 976, 1042
BstV2I GAAGAC 3 cut(s) 374, 419, 1107
BstX2I RGATCY 1 cut(s) 713
BstYI RGATCY 1 cut(s) 713
BsuRI GGCC 2 cut(s) 5, 315
BtsCI GGATG 4 cut(s) 26, 622, 1079, 1177
BtsI GCAGTG 3 cut(s) 513, 574, 1215
BtsIMutI CAGTG 6 cut(s) 211, 265, 513, 574, 1095, 1215
CaiI CAGNNNCTG 1 cut(s) 869
CfoI GCGC 1 cut(s) 186
CseI GACGC 2 cut(s) 512, 963
Csp6I GTAC 3 cut(s) 658, 1083, 1126
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 8 cut(s) 87, 269, 317, 605, 641, 706, 818, 1030
CviQI GTAC 3 cut(s) 658, 1083, 1126
DdeI CTNAG 2 cut(s) 352, 444
DpnI GATC 5 cut(s) 245, 460, 715, 753, 981
DpnII GATC 5 cut(s) 243, 458, 713, 751, 979
EaeI YGGCCR 2 cut(s) 3, 313
Eam1104I CTCTTC 3 cut(s) 44, 109, 139
EarI CTCTTC 3 cut(s) 44, 109, 139
Eco24I GRGCYC 1 cut(s) 311
Eco32I GATATC 1 cut(s) 1066
Eco57I CTGAAG 3 cut(s) 172, 187, 1119
Eco91I GGTNACC 1 cut(s) 322
EcoO65I GGTNACC 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 784
EcoRV GATATC 1 cut(s) 1066
EcoT38I GRGCYC 1 cut(s) 311
FaeI CATG 8 cut(s) 90, 272, 320, 608, 644, 709, 821, 1033
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FaqI GGGAC 3 cut(s) 779, 839, 1174
FatI CATG 8 cut(s) 86, 268, 316, 604, 640, 705, 817, 1029
Fnu4HI GCNGC 5 cut(s) 222, 870, 948, 990, 1056
FokI GGATG 4 cut(s) 13, 629, 1086, 1184
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 5 cut(s) 222, 870, 948, 990, 1056
FspBI CTAG 2 cut(s) 195, 848
GlaI GCGC 1 cut(s) 185
GluI GCNGC 5 cut(s) 222, 870, 948, 990, 1056
GsaI CCCAGC 2 cut(s) 576, 996
GsuI CTGGAG 2 cut(s) 426, 1205
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 2 cut(s) 5, 315
HapII CCGG 3 cut(s) 717, 792, 959
HgaI GACGC 2 cut(s) 512, 963
HhaI GCGC 1 cut(s) 186
Hin1I GRCGYC 1 cut(s) 955
Hin1II CATG 8 cut(s) 90, 272, 320, 608, 644, 709, 821, 1033
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 1 cut(s) 1011
HinfI GANTC 4 cut(s) 282, 384, 913, 1187
HpaII CCGG 3 cut(s) 717, 792, 959
HphI GGTGA 4 cut(s) 316, 353, 370, 973
Hpy166II GTNNAC 3 cut(s) 250, 698, 964
Hpy188I TCNGA 3 cut(s) 243, 355, 391
Hpy188III TCNNGA 2 cut(s) 792, 917
Hpy8I GTNNAC 3 cut(s) 250, 698, 964
HpyAV CCTTC 5 cut(s) 81, 93, 211, 449, 605
HpyCH4III ACNGT 6 cut(s) 260, 337, 418, 808, 893, 905
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 221, 440, 527, 947, 1010
HpyF3I CTNAG 2 cut(s) 352, 444
Hsp92I GRCGYC 1 cut(s) 955
Hsp92II CATG 8 cut(s) 90, 272, 320, 608, 644, 709, 821, 1033
HspAI GCGC 1 cut(s) 184
Kpn2I TCCGGA 1 cut(s) 791
Kzo9I GATC 5 cut(s) 243, 458, 713, 751, 979
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 2 cut(s) 595, 794
Lsp1109I GCAGC 5 cut(s) 208, 856, 934, 976, 1042
LweI GCATC 4 cut(s) 622, 856, 881, 991
MaeI CTAG 2 cut(s) 195, 848
MaeIII GTNAC 4 cut(s) 160, 322, 910, 1025
MalI GATC 5 cut(s) 245, 460, 715, 753, 981
MboI GATC 5 cut(s) 243, 458, 713, 751, 979
MflI RGATCY 1 cut(s) 713
MhlI GDGCHC 3 cut(s) 39, 311, 943
MlsI TGGCCA 2 cut(s) 5, 315
MluCI AATT 5 cut(s) 393, 551, 679, 756, 1160
MluNI TGGCCA 2 cut(s) 5, 315
MlyI GAGTC 2 cut(s) 907, 1196
MmeI TCCRAC 2 cut(s) 81, 459
Mox20I TGGCCA 2 cut(s) 5, 315
MroI TCCGGA 1 cut(s) 791
MscI TGGCCA 2 cut(s) 5, 315
MseI TTAA 2 cut(s) 624, 1194
MslI CAYNNNNRTG 2 cut(s) 329, 1038
Msp20I TGGCCA 2 cut(s) 5, 315
MspI CCGG 3 cut(s) 717, 792, 959
MspR9I CCNGG 2 cut(s) 786, 959
Mva1269I GAATGC 1 cut(s) 801
MvaI CCWGG 1 cut(s) 786
MwoI GCNNNNNNNGC 6 cut(s) 11, 221, 440, 527, 947, 1010
NciI CCSGG 1 cut(s) 959
NdeII GATC 5 cut(s) 243, 458, 713, 751, 979
NlaIII CATG 8 cut(s) 90, 272, 320, 608, 644, 709, 821, 1033
NlaIV GGNNCC 3 cut(s) 597, 715, 790
NmuCI GTSAC 3 cut(s) 160, 322, 910
NspI RCATGY 1 cut(s) 1033
PciSI GCTCTTC 1 cut(s) 44
PctI GAATGC 1 cut(s) 801
PfeI GAWTC 2 cut(s) 282, 384
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 5 cut(s) 223, 871, 949, 991, 1057
Ple19I CGATCG 1 cut(s) 754
PleI GAGTC 2 cut(s) 907, 1195
PpsI GAGTC 2 cut(s) 907, 1195
PsiI TTATAA 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 322
PspFI CCCAGC 2 cut(s) 572, 992
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 3 cut(s) 597, 715, 790
PstI CTGCAG 2 cut(s) 226, 952
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 1 cut(s) 713
PvuI CGATCG 1 cut(s) 754
RsaI GTAC 3 cut(s) 659, 1084, 1127
RsaNI GTAC 3 cut(s) 658, 1083, 1126
RseI CAYNNNNRTG 2 cut(s) 329, 1038
SapI GCTCTTC 1 cut(s) 44
SaqAI TTAA 2 cut(s) 624, 1194
SatI GCNGC 5 cut(s) 222, 870, 948, 990, 1056
Sau3AI GATC 5 cut(s) 243, 458, 713, 751, 979
ScaI AGTACT 1 cut(s) 1127
SchI GAGTC 2 cut(s) 907, 1196
ScrFI CCNGG 2 cut(s) 786, 959
SduI GDGCHC 3 cut(s) 39, 311, 943
SfaNI GCATC 4 cut(s) 622, 856, 881, 991
SfcI CTRYAG 2 cut(s) 222, 948
SmiMI CAYNNNNRTG 2 cut(s) 329, 1038
SmlI CTYRAG 1 cut(s) 942
SmoI CTYRAG 1 cut(s) 942
Sse9I AATT 5 cut(s) 393, 551, 679, 756, 1160
SspI AATATT 1 cut(s) 883
SspMI CTAG 2 cut(s) 195, 848
StyD4I CCNGG 2 cut(s) 784, 957
TaaI ACNGT 6 cut(s) 260, 337, 418, 808, 893, 905
TaqI TCGA 4 cut(s) 609, 750, 754, 918
TasI AATT 5 cut(s) 393, 551, 679, 756, 1160
TatI WGTACW 2 cut(s) 657, 1125
TfiI GAWTC 2 cut(s) 282, 384
Tru1I TTAA 2 cut(s) 624, 1194
Tru9I TTAA 2 cut(s) 624, 1194
TscAI CASTG 6 cut(s) 211, 265, 513, 574, 1102, 1215
TseFI GTSAC 3 cut(s) 160, 322, 910
TseI GCWGC 5 cut(s) 221, 869, 947, 989, 1055
Tsp45I GTSAC 3 cut(s) 160, 322, 910
TspDTI ATGAA 3 cut(s) 6, 75, 377
TspGWI ACGGA 2 cut(s) 1008, 1013
TspRI CASTG 6 cut(s) 211, 265, 513, 574, 1102, 1215
Van91I CCANNNNNTGG 1 cut(s) 785
XapI RAATTY 1 cut(s) 1160
XceI RCATGY 1 cut(s) 1033
XspI CTAG 2 cut(s) 195, 848
ZrmI AGTACT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.