Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0010031

Physical Location & Seq

Genomic Coordinates Reverse (-)

317161 .. 333837

Gene Structure 16677 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1003000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGCAAGGAGGATCATCGGGGATCGGGTATGGTCTGAAATACCAGGCTAGATGTATATCAGATGTTAAAGCCGACACAGATCATACTAGCTTCATCACCGGCACTCTCAGCCTCAAAGAAGAAAACGAGGTGCATTTGATTCGGCTTTCTTCCGATGGAACCGAACTCGTGTGCGAGGGTTTGTTTTCTCACCCCAACGAGATCTGGGACCTTGCTTCCTGTCCCTTCGACCAACGCATCTTCTCTACTGTTTACTCTACTGGCGAGTCATACGGAGCAGCAATATGGCAGATCCCCGAGTTATACGGTGAATTGAATTCCCCTCAGTTGGAAAGAATTACCTCCCTCGACTCACAATTTGGCAAGATCAAGTGCATTCTTTGGTGGCCATCGGGAAGGCATGATAAGTTGATCAGCATTGATGAAGAAAATTTGTCCTTGTGGAGCTTAGATTTATCCAAAAAAGCGGCTCAGGTACAATCACAGGAGTCAGCTGGTGTGATGCACTACTTGTCTGGTGGTGCATGGGATCCACATGATATCAATGCTGTTGCAGCAACATGTGAATCATCCATTCATTTTTGGGATCTACGAACAATGAAGAAGACAAATTCTATTGAGCATGCTCACGTTCGCAATGTTGATTATAACCCCAACAAGAAACACGTGCTTATAACTGCAGAAGATGAATCTGGTATACATGTATGGGATCTTAGGAAGCCAAAAGTGCCTATCCAGGAGCTTCCAGGACACACACACTGGACATGGGCTGCCCGGTATAACCCTGAGTACGATGGGCTGATTTTGAGTTCCGGTACAGACTCAGCTGTCAACCTGTGGTTTGCTTCTCCATATAACAATGATGAGTCTGAACGCCTGGTTGACTCGCCTACTAGGCGGATTGATCCATTGCTCAACTCTTACAGTGACTATGAAGATAGTATTTATGGTCTTGCATGGAGCTCTCGTGAGCCTTGGATCTTTGCATCATTATCCTATGATGGGAGGGTAGTTGTAGAATCAGTGAAGCCATTTCTCTCCAAGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010646 GO:0010648 GO:0016567 GO:0019538 GO:0023051 GO:0023057 GO:0031461 GO:0032446 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0065007 GO:0070647 GO:0071704 GO:0080008 GO:1901419 GO:1901420 GO:1901564 GO:1902494 GO:1905957 GO:1905958 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

350

Amino Acids

39.36

Weight (kDa)

5.16

Isoelectric Point (pI)

39.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_EIPR1	PF23609	5 - 280	1.8e-101	EIPR1 beta-propeller
WD40_Prp19	PF24814	83 - 243	2.5e-07	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	108 - 282	1e-13	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	146 - 330	3.5e-10	CDC20/Fizzy WD40 domain
Beta-prop_RIG_2nd	PF23775	162 - 272	9.4e-06	RIG second beta-propeller
Beta-prop_WDR3_2nd	PF25172	181 - 291	6.7e-06	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	184 - 280	2.2e-08	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	211 - 281	1.4e-07	WDR5 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20540 AT1G76260
fragaria_vesca	FvH4_2g37550 FvH4_2g37550 FvH4_2g37550
malus_domestica	MD08G1003000.v1.1 MD15G1002700.v1.1
prunus_persica	Prupe.1G355000_v2.0.a1 Prupe.1G355000_v2.0.a1 Prupe.1G355000_v2.0.a1
pyrus_communis	pycom15g00160
rosa_chinensis	RchiOBHm_Chr6g0304181
rosa_laevigata	RLG00000011008
rosa_multiflora	Rmu_ssc0000218.1_g000010
rosa_roxburghii	Rroxscaffold_7G00163990
rosa_rugosa	Rorug06G0328200 Rorug06G0328300
rosa_samantha	Rh6AG442400 Rh6BG460300 Rh6CG454600
rosa_wichuraiana	Rw6G038350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	648, 674
AasI	GACNNNNNNGTC	1 cut(s)	827
AccI	GTMKAC	1 cut(s)	697
AciI	CCGC	2 cut(s)	467, 898
AclWI	GGATC	9 cut(s)	19, 29, 286, 524, 537, 594, 717, 899, 986
AcoI	YGGCCR	1 cut(s)	386
AcsI	RAATTY	3 cut(s)	316, 430, 610
AcvI	CACGTG	1 cut(s)	667
AfaI	GTAC	3 cut(s)	477, 791, 817
AfiI	CCNNNNNNNGG	2 cut(s)	328, 1002
AflIII	ACRYGT	3 cut(s)	560, 664, 700
AgsI	TTSAA	1 cut(s)	316
AjnI	CCWGG	4 cut(s)	42, 735, 745, 876
AluBI	AGCT	6 cut(s)	90, 447, 494, 742, 827, 963
AluI	AGCT	6 cut(s)	90, 447, 494, 742, 827, 963
Alw21I	GWGCWC	1 cut(s)	965
AlwI	GGATC	9 cut(s)	19, 29, 286, 524, 537, 594, 717, 899, 986
Ama87I	CYCGRG	1 cut(s)	296
AoxI	GGCC	1 cut(s)	386
ApeKI	GCWGC	3 cut(s)	278, 554, 770
ApoI	RAATTY	3 cut(s)	316, 430, 610
ArsI	GACNNNNNNTTYG	2 cut(s)	341, 373
AspS9I	GGNCC	1 cut(s)	208
AsuC2I	CCSGG	1 cut(s)	775
AsuHPI	GGTGA	3 cut(s)	88, 182, 320
AvaI	CYCGRG	1 cut(s)	296
AvaII	GGWCC	1 cut(s)	208
BalI	TGGCCA	1 cut(s)	388
BamHI	GGATCC	1 cut(s)	529
BanII	GRGCYC	1 cut(s)	965
BauI	CACGAG	2 cut(s)	167, 966
BbrPI	CACGTG	1 cut(s)	667
BbsI	GAAGAC	1 cut(s)	611
Bbv12I	GWGCWC	1 cut(s)	965
BbvI	GCAGC	3 cut(s)	290, 566, 757
BccI	CCATC	4 cut(s)	149, 397, 788, 995
BcgI	CGANNNNNNTGC	2 cut(s)	122, 156
BciT130I	CCWGG	4 cut(s)	44, 737, 747, 878
BclI	TGATCA	1 cut(s)	411
BcnI	CCSGG	1 cut(s)	775
BfaI	CTAG	3 cut(s)	48, 87, 894
BfmI	CTRYAG	1 cut(s)	678
BglI	GCCNNNNNGGC	1 cut(s)	895
BglII	AGATCT	1 cut(s)	201
BisI	GCNGC	4 cut(s)	279, 468, 555, 771
BlsI	GCNGC	4 cut(s)	280, 469, 556, 772
Bme1390I	CCNGG	5 cut(s)	44, 737, 747, 775, 878
Bme18I	GGWCC	1 cut(s)	208
BmeT110I	CYCGRG	1 cut(s)	296
BmgT120I	GGNCC	1 cut(s)	208
BmiI	GGNNCC	3 cut(s)	160, 209, 531
BmrFI	CCNGG	5 cut(s)	44, 737, 747, 775, 878
BmsI	GCATC	3 cut(s)	246, 492, 995
BpiI	GAAGAC	1 cut(s)	611
Bpu10I	CCTNAGC	1 cut(s)	471
BpuMI	CCSGG	1 cut(s)	775
BsaAI	YACGTR	1 cut(s)	667
BsaBI	GATNNNNATC	1 cut(s)	55
BsaJI	CCNNGG	1 cut(s)	974
BsaWI	WCCGGW	1 cut(s)	812
BsaXI	ACNNNNNCTCC	2 cut(s)	952, 982
Bsc4I	CCNNNNNNNGG	2 cut(s)	328, 1002
Bse118I	RCCGGY	1 cut(s)	98
Bse1I	ACTGG	2 cut(s)	265, 764
Bse3DI	GCAATG	2 cut(s)	643, 908
Bse8I	GATNNNNATC	1 cut(s)	55
BseBI	CCWGG	4 cut(s)	44, 737, 747, 878
BseDI	CCNNGG	1 cut(s)	974
BseGI	GGATG	1 cut(s)	569
BseJI	GATNNNNATC	1 cut(s)	55
BseLI	CCNNNNNNNGG	2 cut(s)	328, 1002
BseMI	GCAATG	2 cut(s)	643, 908
BseMII	CTCAG	5 cut(s)	121, 338, 485, 777, 837
BseNI	ACTGG	2 cut(s)	265, 764
BseXI	GCAGC	3 cut(s)	290, 566, 757
BshFI	GGCC	1 cut(s)	388
BsiHKAI	GWGCWC	1 cut(s)	965
BsiHKCI	CYCGRG	1 cut(s)	296
BsiSI	CCGG	3 cut(s)	99, 775, 813
BslFI	GGGAC	2 cut(s)	207, 221
BslI	CCNNNNNNNGG	2 cut(s)	328, 1002
BsmFI	GGGAC	2 cut(s)	207, 221
BsmI	GAATGC	1 cut(s)	375
BsnI	GGCC	1 cut(s)	388
BsoBI	CYCGRG	1 cut(s)	296
Bsp1286I	GDGCHC	1 cut(s)	965
BspACI	CCGC	2 cut(s)	467, 898
BspANI	GGCC	1 cut(s)	388
BspCNI	CTCAG	5 cut(s)	120, 337, 484, 778, 836
BspLI	GGNNCC	3 cut(s)	160, 209, 531
BspMAI	CTGCAG	1 cut(s)	682
BspPI	GGATC	9 cut(s)	19, 29, 286, 524, 537, 594, 717, 899, 986
BsrDI	GCAATG	2 cut(s)	643, 908
BsrFI	RCCGGY	1 cut(s)	98
BsrI	ACTGG	2 cut(s)	265, 764
BssAI	RCCGGY	1 cut(s)	98
BssECI	CCNNGG	1 cut(s)	974
BssNAI	GTATAC	1 cut(s)	698
BssSI	CACGAG	2 cut(s)	167, 966
BssT1I	CCWWGG	1 cut(s)	974
Bst1107I	GTATAC	1 cut(s)	698
Bst2BI	CACGAG	2 cut(s)	167, 966
Bst2UI	CCWGG	4 cut(s)	44, 737, 747, 878
Bst4CI	ACNGT	3 cut(s)	250, 308, 926
BstBAI	YACGTR	1 cut(s)	667
BstC8I	GCNNGC	1 cut(s)	624
BstDEI	CTNAG	7 cut(s)	107, 324, 448, 471, 713, 786, 823
BstF5I	GGATG	1 cut(s)	569
BstMWI	GCNNNNNNNGC	4 cut(s)	108, 554, 727, 895
BstNI	CCWGG	4 cut(s)	44, 737, 747, 878
BstNSI	RCATGY	3 cut(s)	564, 626, 704
BstSCI	CCNGG	5 cut(s)	42, 735, 745, 773, 876
BstSFI	CTRYAG	1 cut(s)	678
BstV1I	GCAGC	3 cut(s)	290, 566, 757
BstV2I	GAAGAC	1 cut(s)	611
BstX2I	RGATCY	6 cut(s)	201, 291, 529, 586, 709, 978
BstYI	RGATCY	6 cut(s)	201, 291, 529, 586, 709, 978
BstZ17I	GTATAC	1 cut(s)	698
BsuRI	GGCC	1 cut(s)	388
BtsCI	GGATG	1 cut(s)	569
BtsIMutI	CAGTG	3 cut(s)	757, 931, 1029
Cac8I	GCNNGC	1 cut(s)	624
Cfr10I	RCCGGY	1 cut(s)	98
Cfr13I	GGNCC	1 cut(s)	208
Csp6I	GTAC	3 cut(s)	476, 790, 816
CviAII	CATG	8 cut(s)	401, 525, 536, 561, 623, 701, 765, 957
CviQI	GTAC	3 cut(s)	476, 790, 816
DdeI	CTNAG	7 cut(s)	107, 324, 448, 471, 713, 786, 823
DrdI	GACNNNNNNGTC	1 cut(s)	827
DseDI	GACNNNNNNGTC	1 cut(s)	827
EaeI	YGGCCR	1 cut(s)	386
EciI	GGCGGA	1 cut(s)	913
Ecl136II	GAGCTC	1 cut(s)	963
Eco130I	CCWWGG	1 cut(s)	974
Eco24I	GRGCYC	1 cut(s)	965
Eco32I	GATATC	1 cut(s)	541
Eco47I	GGWCC	1 cut(s)	208
Eco53kI	GAGCTC	1 cut(s)	963
Eco72I	CACGTG	1 cut(s)	667
Eco88I	CYCGRG	1 cut(s)	296
EcoICRI	GAGCTC	1 cut(s)	963
EcoO109I	RGGNCCY	1 cut(s)	208
EcoRI	GAATTC	1 cut(s)	316
EcoRII	CCWGG	4 cut(s)	42, 735, 745, 876
EcoRV	GATATC	1 cut(s)	541
EcoT14I	CCWWGG	1 cut(s)	974
EcoT38I	GRGCYC	1 cut(s)	965
ErhI	CCWWGG	1 cut(s)	974
FaeI	CATG	8 cut(s)	404, 528, 539, 564, 626, 704, 768, 960
FaqI	GGGAC	2 cut(s)	207, 221
FatI	CATG	8 cut(s)	400, 524, 535, 560, 622, 700, 764, 956
FbaI	TGATCA	1 cut(s)	411
FblI	GTMKAC	1 cut(s)	697
Fnu4HI	GCNGC	4 cut(s)	279, 468, 555, 771
FokI	GGATG	1 cut(s)	556
FriOI	GRGCYC	1 cut(s)	965
Fsp4HI	GCNGC	4 cut(s)	279, 468, 555, 771
FspBI	CTAG	3 cut(s)	48, 87, 894
GluI	GCNGC	4 cut(s)	279, 468, 555, 771
HaeIII	GGCC	1 cut(s)	388
HapII	CCGG	3 cut(s)	99, 775, 813
Hin1II	CATG	8 cut(s)	404, 528, 539, 564, 626, 704, 768, 960
HincII	GTYRAC	2 cut(s)	832, 883
HindII	GTYRAC	2 cut(s)	832, 883
HpaII	CCGG	3 cut(s)	99, 775, 813
HphI	GGTGA	3 cut(s)	88, 182, 320
Hpy166II	GTNNAC	4 cut(s)	253, 698, 832, 883
Hpy188I	TCNGA	4 cut(s)	36, 61, 154, 871
Hpy188III	TCNNGA	2 cut(s)	393, 968
Hpy8I	GTNNAC	4 cut(s)	253, 698, 832, 883
HpyAV	CCTTC	2 cut(s)	235, 390
HpyCH4III	ACNGT	3 cut(s)	250, 308, 926
HpyCH4IV	ACGT	2 cut(s)	630, 666
HpyCH4V	TGCA	9 cut(s)	4, 133, 375, 505, 524, 554, 680, 956, 986
HpyF10VI	GCNNNNNNNGC	4 cut(s)	108, 554, 727, 895
HpyF3I	CTNAG	7 cut(s)	107, 324, 448, 471, 713, 786, 823
HpySE526I	ACGT	2 cut(s)	630, 666
Hsp92II	CATG	8 cut(s)	404, 528, 539, 564, 626, 704, 768, 960
Ksp22I	TGATCA	1 cut(s)	411
LmnI	GCTCC	4 cut(s)	275, 444, 739, 960
Lsp1109I	GCAGC	3 cut(s)	290, 566, 757
LweI	GCATC	3 cut(s)	246, 492, 995
MaeI	CTAG	3 cut(s)	48, 87, 894
MaeII	ACGT	2 cut(s)	630, 666
MaeIII	GTNAC	1 cut(s)	926
MboII	GAAGA	8 cut(s)	131, 141, 232, 437, 613, 616, 695, 947
MflI	RGATCY	6 cut(s)	201, 291, 529, 586, 709, 978
MhlI	GDGCHC	1 cut(s)	965
MlsI	TGGCCA	1 cut(s)	388
MluCI	AATT	6 cut(s)	311, 316, 336, 356, 430, 610
MluNI	TGGCCA	1 cut(s)	388
MlyI	GAGTC	6 cut(s)	275, 344, 497, 815, 875, 878
MmeI	TCCRAC	1 cut(s)	309
MnlI	CCTC	7 cut(s)	121, 122, 169, 333, 352, 356, 999
Mox20I	TGGCCA	1 cut(s)	388
MscI	TGGCCA	1 cut(s)	388
MseI	TTAA	1 cut(s)	66
Msp20I	TGGCCA	1 cut(s)	388
MspA1I	CMGCKG	2 cut(s)	494, 827
MspI	CCGG	3 cut(s)	99, 775, 813
MspR9I	CCNGG	5 cut(s)	44, 737, 747, 775, 878
Mva1269I	GAATGC	1 cut(s)	375
MvaI	CCWGG	4 cut(s)	44, 737, 747, 878
MwoI	GCNNNNNNNGC	4 cut(s)	108, 554, 727, 895
NciI	CCSGG	1 cut(s)	775
NlaIII	CATG	8 cut(s)	404, 528, 539, 564, 626, 704, 768, 960
NlaIV	GGNNCC	3 cut(s)	160, 209, 531
NmuCI	GTSAC	1 cut(s)	926
NspI	RCATGY	3 cut(s)	564, 626, 704
PaeI	GCATGC	1 cut(s)	626
PciI	ACATGT	2 cut(s)	560, 700
PctI	GAATGC	1 cut(s)	375
PfeI	GAWTC	4 cut(s)	139, 566, 689, 1019
PfoI	TCCNGGA	2 cut(s)	735, 745
PkrI	GCNGC	4 cut(s)	280, 469, 556, 772
PleI	GAGTC	6 cut(s)	274, 344, 496, 815, 874, 878
PmaCI	CACGTG	1 cut(s)	667
PmlI	CACGTG	1 cut(s)	667
PpsI	GAGTC	6 cut(s)	274, 344, 496, 815, 874, 878
Ppu21I	YACGTR	1 cut(s)	667
PpuMI	RGGWCCY	1 cut(s)	208
PscI	ACATGT	2 cut(s)	560, 700
PsiI	TTATAA	2 cut(s)	648, 674
Psp124BI	GAGCTC	1 cut(s)	965
Psp5II	RGGWCCY	1 cut(s)	208
Psp6I	CCWGG	4 cut(s)	42, 735, 745, 876
PspCI	CACGTG	1 cut(s)	667
PspGI	CCWGG	4 cut(s)	42, 735, 745, 876
PspN4I	GGNNCC	3 cut(s)	160, 209, 531
PspPI	GGNCC	1 cut(s)	208
PspPPI	RGGWCCY	1 cut(s)	208
PstI	CTGCAG	1 cut(s)	682
PsuI	RGATCY	6 cut(s)	201, 291, 529, 586, 709, 978
PvuII	CAGCTG	2 cut(s)	494, 827
RsaI	GTAC	3 cut(s)	477, 791, 817
RsaNI	GTAC	3 cut(s)	476, 790, 816
SacI	GAGCTC	1 cut(s)	965
SaqAI	TTAA	1 cut(s)	66
SatI	GCNGC	4 cut(s)	279, 468, 555, 771
Sau96I	GGNCC	1 cut(s)	208
SchI	GAGTC	6 cut(s)	275, 344, 497, 815, 875, 878
ScrFI	CCNGG	5 cut(s)	44, 737, 747, 775, 878
SduI	GDGCHC	1 cut(s)	965
SfaNI	GCATC	3 cut(s)	246, 492, 995
SfcI	CTRYAG	1 cut(s)	678
SinI	GGWCC	1 cut(s)	208
SphI	GCATGC	1 cut(s)	626
Sse9I	AATT	6 cut(s)	311, 316, 336, 356, 430, 610
SsiI	CCGC	2 cut(s)	467, 898
SspMI	CTAG	3 cut(s)	48, 87, 894
SstI	GAGCTC	1 cut(s)	965
StyD4I	CCNGG	5 cut(s)	42, 735, 745, 773, 876
StyI	CCWWGG	1 cut(s)	974
TaaI	ACNGT	3 cut(s)	250, 308, 926
TaiI	ACGT	2 cut(s)	633, 669
TaqI	TCGA	2 cut(s)	228, 348
TasI	AATT	6 cut(s)	311, 316, 336, 356, 430, 610
TauI	GCSGC	1 cut(s)	470
TfiI	GAWTC	4 cut(s)	139, 566, 689, 1019
Tru1I	TTAA	1 cut(s)	66
Tru9I	TTAA	1 cut(s)	66
TscAI	CASTG	3 cut(s)	764, 931, 1029
TseFI	GTSAC	1 cut(s)	926
TseI	GCWGC	3 cut(s)	278, 554, 770
Tsp45I	GTSAC	1 cut(s)	926
TspDTI	ATGAA	6 cut(s)	82, 438, 566, 614, 702, 948
TspGWI	ACGGA	1 cut(s)	288
TspRI	CASTG	3 cut(s)	764, 931, 1029
VpaK11BI	GGWCC	1 cut(s)	208
XapI	RAATTY	3 cut(s)	316, 430, 610
XceI	RCATGY	3 cut(s)	564, 626, 704
XmiI	GTMKAC	1 cut(s)	697
XspI	CTAG	3 cut(s)	48, 87, 894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.