AT1G20540

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010031
Physical Location & Seq
Forward (+)
7112611 .. 7115362
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20540.1

Sequence Viewer

Length: 1056 bp
ATGCAAGGAGGATCATCGGGTATTGGATACGGTTTGAAGTATCAGTCTAGATGTATATCGGATGTGAAAGCCGATACGGATTACACAAGCTTTCTCACTGGAACTCTTAGTTTAAAAGAAGAAAATGAGGTTCATCTTCTTCGTTTGTCATCTGGTGGATCTGAGCTTATATGTGAGGGTTTGTTCTCTCATCCTAATGAGATTTGGGACCTTGCTTCTTGCCCTTTTGATCAACGCATTTTCTCCACCGTCTTCTCCACTGGTGAATCGTTTGGAGCAGCGATATGGCAAATTCCTGAGTTATATGGTCAACTGAATTCTCCACAATTGGAGCGTGTTGCTTCTCTTGATGCTCATGTTGGTAAGATTAATTGTGTTCTTTGGTGGCCTTCTGGAAGATGTGACAAGTTAATCAGCATTGATGAACAAAATATTTTCTTGTGGAGCTTAGACTGTTCGAAAAAGTCTGCTGAGGTTCTATCTAAGGATTCAGCTGGGATGTTGCATTCTTTATCTGGTGGAGCATGGGATCCACATGATGTAAATGCTGTTGCAGCAACGGGTGAATCATCTGTTCAGTTCTGGGACTTGCGTACTATGAAGAAGGTTAACTCAATTGAACATGCTCATGTACGCGGTGTTGATTACAATCCCAAGAGAGAACATATACTTGTAACTGCAGAGGATGAATCTGGCATACATGTCTGGGATCTCCGGAAGGCCAAGGTTCCTGTCCAAGAGCTTCCTGGTCATACACATTGGACTTGGGCTGTCAGGTGTAACCCTGAGTATGATGGACTGATTCTGAGCGCTGGAACAGATTCGGTTGTCAACCTGTGGTATGCTTCAGCAACATCCAGTGATGACAAAACCTCAGAAAGTCCTGTGGAATCCACACGACAGCGTGTGAACCCGTTGCTCAACTCATACACTGACTATGAAGACAGTGTCTATGGCCTTGCTTGGAGTTCACGTGAGCCTTGGGTTTTTGCATCATTATCATATGACGGAAGAGTCATCATCGAATCCGTCAAGCCTTTCCTTCCAAGAATATAG

Protein Analysis

351

Amino Acids

39.04

Weight (kDa)

5.09

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 5 - 280 2.9e-99 EIPR1 beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 80 - 242 1.7e-06 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 84 - 243 9.7e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 107 - 282 3e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 116 - 283 7.7e-12 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 143 - 332 5.3e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 180 - 332 1.4e-08 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 184 - 281 4e-08 WDR3 first beta-propeller domain
WDR55 PF24796 185 - 276 4.1e-06 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1013
AccII CGCG 1 cut(s) 636
AccIII TCCGGA 1 cut(s) 714
AciI CCGC 1 cut(s) 636
AclWI GGATC 5 cut(s) 19, 166, 524, 537, 717
AcsI RAATTY 2 cut(s) 291, 316
AcuI CTGAAG 1 cut(s) 831
AcvI CACGTG 1 cut(s) 974
AfaI GTAC 2 cut(s) 595, 633
AfeI AGCGCT 1 cut(s) 811
AflIII ACRYGT 1 cut(s) 700
AgsI TTSAA 2 cut(s) 37, 620
AjnI CCWGG 1 cut(s) 745
AluBI AGCT 5 cut(s) 90, 166, 447, 494, 742
AluI AGCT 5 cut(s) 90, 166, 447, 494, 742
AlwI GGATC 5 cut(s) 19, 166, 524, 537, 717
Aor13HI TCCGGA 1 cut(s) 714
Aor51HI AGCGCT 1 cut(s) 811
AoxI GGCC 3 cut(s) 386, 720, 955
ApeKI GCWGC 2 cut(s) 278, 554
ApoI RAATTY 2 cut(s) 291, 316
AseI ATTAAT 1 cut(s) 369
Asp700I GAANNNNTTC 1 cut(s) 820
AspLEI GCGC 1 cut(s) 812
AspS9I GGNCC 1 cut(s) 208
AsuHPI GGTGA 2 cut(s) 275, 575
AsuII TTCGAA 1 cut(s) 458
AvaII GGWCC 1 cut(s) 208
BamHI GGATCC 1 cut(s) 529
BbrPI CACGTG 1 cut(s) 974
BbsI GAAGAC 2 cut(s) 244, 948
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 290, 566
BccI CCATC 1 cut(s) 788
BciT130I CCWGG 1 cut(s) 747
BciVI GTATCC 1 cut(s) 20
BclI TGATCA 1 cut(s) 229
BfaI CTAG 1 cut(s) 48
BfmI CTRYAG 1 cut(s) 678
BfoI RGCGCY 1 cut(s) 813
BfuI GTATCC 1 cut(s) 20
BisI GCNGC 2 cut(s) 279, 555
BlsI GCNGC 2 cut(s) 280, 556
Bme1390I CCNGG 1 cut(s) 747
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 3 cut(s) 209, 531, 729
BmrFI CCNGG 1 cut(s) 747
BmsI GCATC 2 cut(s) 340, 1001
BpiI GAAGAC 2 cut(s) 244, 948
Bpu10I CCTNAGC 1 cut(s) 471
Bpu14I TTCGAA 1 cut(s) 458
BsaAI YACGTR 1 cut(s) 974
BsaBI GATNNNNATC 2 cut(s) 55, 648
BsaJI CCNNGG 2 cut(s) 723, 980
BsaWI WCCGGW 1 cut(s) 714
BsaXI ACNNNNNCTCC 2 cut(s) 958, 988
Bse1I ACTGG 3 cut(s) 103, 265, 858
Bse8I GATNNNNATC 2 cut(s) 55, 648
BseAI TCCGGA 1 cut(s) 714
BseBI CCWGG 1 cut(s) 747
BseDI CCNNGG 2 cut(s) 723, 980
BseGI GGATG 5 cut(s) 67, 190, 504, 691, 854
BseJI GATNNNNATC 2 cut(s) 55, 648
BseMII CTCAG 6 cut(s) 153, 288, 462, 777, 797, 888
BseNI ACTGG 3 cut(s) 103, 265, 858
BseXI GCAGC 2 cut(s) 290, 566
BseYI CCCAGC 1 cut(s) 494
Bsh1236I CGCG 1 cut(s) 636
BshFI GGCC 3 cut(s) 388, 722, 957
BsiSI CCGG 1 cut(s) 715
BslFI GGGAC 2 cut(s) 221, 599
BsmFI GGGAC 2 cut(s) 221, 599
BsmI GAATGC 1 cut(s) 505
BsnI GGCC 3 cut(s) 388, 722, 957
Bsp119I TTCGAA 1 cut(s) 458
Bsp13I TCCGGA 1 cut(s) 714
Bsp143I GATC 5 cut(s) 11, 158, 229, 529, 709
BspACI CCGC 1 cut(s) 636
BspANI GGCC 3 cut(s) 388, 722, 957
BspCNI CTCAG 6 cut(s) 154, 289, 463, 778, 798, 887
BspEI TCCGGA 1 cut(s) 714
BspFNI CGCG 1 cut(s) 636
BspLI GGNNCC 3 cut(s) 209, 531, 729
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 5 cut(s) 19, 166, 524, 537, 717
BspT104I TTCGAA 1 cut(s) 458
BsrI ACTGG 3 cut(s) 103, 265, 858
BssECI CCNNGG 2 cut(s) 723, 980
BssMI GATC 5 cut(s) 11, 158, 229, 529, 709
BssT1I CCWWGG 2 cut(s) 723, 980
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 4 cut(s) 32, 250, 455, 947
Bst6I CTCTTC 1 cut(s) 1006
BstBAI YACGTR 1 cut(s) 974
BstBI TTCGAA 1 cut(s) 458
BstDEI CTNAG 9 cut(s) 107, 162, 297, 448, 471, 483, 786, 806, 874
BstF5I GGATG 5 cut(s) 67, 190, 504, 691, 854
BstFNI CGCG 1 cut(s) 636
BstH2I RGCGCY 1 cut(s) 813
BstHHI GCGC 1 cut(s) 812
BstKTI GATC 5 cut(s) 14, 161, 232, 532, 712
BstMBI GATC 5 cut(s) 11, 158, 229, 529, 709
BstMWI GCNNNNNNNGC 1 cut(s) 554
BstNI CCWGG 1 cut(s) 747
BstNSI RCATGY 2 cut(s) 626, 704
BstSCI CCNGG 1 cut(s) 745
BstSFI CTRYAG 1 cut(s) 678
BstUI CGCG 1 cut(s) 636
BstV1I GCAGC 2 cut(s) 290, 566
BstV2I GAAGAC 2 cut(s) 244, 948
BstX2I RGATCY 3 cut(s) 158, 529, 709
BstYI RGATCY 3 cut(s) 158, 529, 709
BsuI GTATCC 1 cut(s) 20
BsuRI GGCC 3 cut(s) 388, 722, 957
BtsCI GGATG 5 cut(s) 67, 190, 504, 691, 854
BtsIMutI CAGTG 5 cut(s) 96, 258, 865, 930, 952
CfoI GCGC 1 cut(s) 812
Cfr13I GGNCC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 594, 632
CviAII CATG 6 cut(s) 356, 525, 536, 623, 629, 701
CviQI GTAC 2 cut(s) 594, 632
DdeI CTNAG 9 cut(s) 107, 162, 297, 448, 471, 483, 786, 806, 874
DpnI GATC 5 cut(s) 13, 160, 231, 531, 711
DpnII GATC 5 cut(s) 11, 158, 229, 529, 709
DraI TTTAAA 1 cut(s) 114
DrdI GACNNNNNNGTC 1 cut(s) 1013
DseDI GACNNNNNNGTC 1 cut(s) 1013
Eam1104I CTCTTC 1 cut(s) 1006
EarI CTCTTC 1 cut(s) 1006
Eco130I CCWWGG 2 cut(s) 723, 980
Eco47I GGWCC 1 cut(s) 208
Eco47III AGCGCT 1 cut(s) 811
Eco57I CTGAAG 1 cut(s) 831
Eco72I CACGTG 1 cut(s) 974
EcoO109I RGGNCCY 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 723, 980
ErhI CCWWGG 2 cut(s) 723, 980
FaeI CATG 6 cut(s) 359, 528, 539, 626, 632, 704
FaqI GGGAC 2 cut(s) 221, 599
FatI CATG 6 cut(s) 355, 524, 535, 622, 628, 700
FauNDI CATATG 1 cut(s) 1003
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 2 cut(s) 279, 555
FokI GGATG 5 cut(s) 74, 177, 511, 698, 841
Fsp4HI GCNGC 2 cut(s) 279, 555
FspBI CTAG 1 cut(s) 48
GlaI GCGC 1 cut(s) 811
GluI GCNGC 2 cut(s) 279, 555
GsaI CCCAGC 1 cut(s) 498
HaeII RGCGCY 1 cut(s) 813
HaeIII GGCC 3 cut(s) 388, 722, 957
HapII CCGG 1 cut(s) 715
HhaI GCGC 1 cut(s) 812
Hin1II CATG 6 cut(s) 359, 528, 539, 626, 632, 704
Hin6I GCGC 1 cut(s) 810
HinP1I GCGC 1 cut(s) 810
HincII GTYRAC 3 cut(s) 311, 610, 832
HindII GTYRAC 3 cut(s) 311, 610, 832
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 9 cut(s) 266, 488, 566, 689, 802, 821, 890, 1014, 1025
HpaI GTTAAC 1 cut(s) 610
HpaII CCGG 1 cut(s) 715
HphI GGTGA 2 cut(s) 275, 575
Hpy166II GTNNAC 5 cut(s) 311, 610, 832, 910, 971
Hpy188I TCNGA 4 cut(s) 61, 163, 807, 877
Hpy188III TCNNGA 5 cut(s) 48, 296, 347, 393, 715
Hpy8I GTNNAC 5 cut(s) 311, 610, 832, 910, 971
HpyAV CCTTC 4 cut(s) 399, 598, 712, 1052
HpyCH4III ACNGT 4 cut(s) 32, 250, 455, 947
HpyCH4IV ACGT 1 cut(s) 973
HpyCH4V TGCA 5 cut(s) 4, 505, 554, 680, 992
HpyF10VI GCNNNNNNNGC 1 cut(s) 554
HpyF3I CTNAG 9 cut(s) 107, 162, 297, 448, 471, 483, 786, 806, 874
HpySE526I ACGT 1 cut(s) 973
Hsp92II CATG 6 cut(s) 359, 528, 539, 626, 632, 704
HspAI GCGC 1 cut(s) 810
Kpn2I TCCGGA 1 cut(s) 714
Ksp22I TGATCA 1 cut(s) 229
KspAI GTTAAC 1 cut(s) 610
Kzo9I GATC 5 cut(s) 11, 158, 229, 529, 709
LmnI GCTCC 4 cut(s) 275, 331, 444, 521
Lsp1109I GCAGC 2 cut(s) 290, 566
LweI GCATC 2 cut(s) 340, 1001
MaeI CTAG 1 cut(s) 48
MaeII ACGT 1 cut(s) 973
MaeIII GTNAC 3 cut(s) 401, 673, 779
MalI GATC 5 cut(s) 13, 160, 231, 531, 711
MboI GATC 5 cut(s) 11, 158, 229, 529, 709
MboII GAAGA 8 cut(s) 128, 131, 131, 244, 408, 613, 953, 1023
MfeI CAATTG 2 cut(s) 326, 615
MflI RGATCY 3 cut(s) 158, 529, 709
MluCI AATT 5 cut(s) 291, 316, 326, 370, 615
MlyI GAGTC 1 cut(s) 1023
MnlI CCTC 5 cut(s) 121, 169, 466, 676, 883
MroI TCCGGA 1 cut(s) 714
MroXI GAANNNNTTC 1 cut(s) 820
MseI TTAA 4 cut(s) 113, 369, 410, 609
MslI CAYNNNNRTG 2 cut(s) 195, 627
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 1 cut(s) 715
MspR9I CCNGG 1 cut(s) 747
MunI CAATTG 2 cut(s) 326, 615
Mva1269I GAATGC 1 cut(s) 505
MvaI CCWGG 1 cut(s) 747
MvnI CGCG 1 cut(s) 636
MwoI GCNNNNNNNGC 1 cut(s) 554
NdeI CATATG 1 cut(s) 1003
NdeII GATC 5 cut(s) 11, 158, 229, 529, 709
NlaIII CATG 6 cut(s) 359, 528, 539, 626, 632, 704
NlaIV GGNNCC 3 cut(s) 209, 531, 729
NmuCI GTSAC 1 cut(s) 401
NspI RCATGY 2 cut(s) 626, 704
NspV TTCGAA 1 cut(s) 458
PciI ACATGT 1 cut(s) 700
PctI GAATGC 1 cut(s) 505
PdmI GAANNNNTTC 1 cut(s) 820
PfeI GAWTC 8 cut(s) 266, 488, 566, 689, 802, 821, 890, 1025
PflFI GACNNNGTC 1 cut(s) 947
PkrI GCNGC 2 cut(s) 280, 556
PleI GAGTC 1 cut(s) 1022
PmaCI CACGTG 1 cut(s) 974
PmlI CACGTG 1 cut(s) 974
PpsI GAGTC 1 cut(s) 1022
Ppu21I YACGTR 1 cut(s) 974
PpuMI RGGWCCY 1 cut(s) 208
PscI ACATGT 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 208
Psp6I CCWGG 1 cut(s) 745
PspCI CACGTG 1 cut(s) 974
PspFI CCCAGC 1 cut(s) 494
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 3 cut(s) 209, 531, 729
PspPI GGNCC 1 cut(s) 208
PspPPI RGGWCCY 1 cut(s) 208
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 3 cut(s) 158, 529, 709
PsyI GACNNNGTC 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 595, 633
RsaNI GTAC 2 cut(s) 594, 632
RseI CAYNNNNRTG 2 cut(s) 195, 627
SaqAI TTAA 4 cut(s) 113, 369, 410, 609
SatI GCNGC 2 cut(s) 279, 555
Sau3AI GATC 5 cut(s) 11, 158, 229, 529, 709
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 1 cut(s) 1023
ScrFI CCNGG 1 cut(s) 747
SfaNI GCATC 2 cut(s) 340, 1001
SfcI CTRYAG 1 cut(s) 678
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 195, 627
Sse9I AATT 5 cut(s) 291, 316, 326, 370, 615
SsiI CCGC 1 cut(s) 636
SspI AATATT 1 cut(s) 433
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 745
StyI CCWWGG 2 cut(s) 723, 980
TaaI ACNGT 4 cut(s) 32, 250, 455, 947
TaiI ACGT 1 cut(s) 976
TaqI TCGA 2 cut(s) 458, 1023
TasI AATT 5 cut(s) 291, 316, 326, 370, 615
TfiI GAWTC 8 cut(s) 266, 488, 566, 689, 802, 821, 890, 1025
Tru1I TTAA 4 cut(s) 113, 369, 410, 609
Tru9I TTAA 4 cut(s) 113, 369, 410, 609
TscAI CASTG 5 cut(s) 103, 265, 865, 937, 952
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 2 cut(s) 278, 554
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 5 cut(s) 122, 438, 614, 702, 954
TspGWI ACGGA 3 cut(s) 92, 1018, 1023
TspRI CASTG 5 cut(s) 103, 265, 865, 937, 952
Tth111I GACNNNGTC 1 cut(s) 947
VpaK11BI GGWCC 1 cut(s) 208
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 2 cut(s) 291, 316
XbaI TCTAGA 1 cut(s) 47
XceI RCATGY 2 cut(s) 626, 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XmnI GAANNNNTTC 1 cut(s) 820
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.