AT1G76270

fucose metabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009884
Physical Location & Seq
Reverse (-)
28613207 .. 28616785
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76270.1

Sequence Viewer

Length: 1719 bp
ATGAATTTCCATCGGCGGCGCTACCCTTACTACAACCGTCTCCGTCGTCTCCTTCCTCTCGTTATCGCCGTCTCTCTATCTCTCCTCATTCTCTTCGCTTTTCTCTCTTTCCTTGCTCCGCCTCCTGGAGATTCCGATCGCCTTCCTCCACGCGTTCGCTACTCATCCAATGATGCGATCAAAGCTACAGGTTTCCATATTCCGAGAGCTGGAGGCAGATCAGATCGTGATATCTGGCGTTCACGAAATGCTGAATTCTTTTTTGGATGCAGCAATGCAAGCAGCAAATTCGCAAATTCGAAAGCAGTGACGAGAAATGATCGATACTTGGTAATAGCCACTAGTGGTGGTTTGAACCAACAACGAACTGGAATTGTAGATGCAGTTGTTGCGGCAAGGATTCTAAATGCTACTCTTGTTGTTCCCAAGTTAGACCAGAAGTCTTACTGGAAGGATGCCAGTGACTTCTCGCATATTTTCGATGTTGATTGGTTTATCTCATTTCTGTCTGGAGACGTCAGAATTATAAAGCAACTTCCACTGAAAGGAGGGCGTACATGGAGTACAAGTAGAATGCGTGTACCACGGAAATGTAACGAGAGATGCTATATTAACCGTGTTTTACCTGTTCTTCTCAAAAGGCATGCAGTTCAACTGAACAAATTTGACTATAGACTTTCAAACAAGTTGAGTGATGACTTGCAAAAGCTAAGATGTAGAGTAAATTACCATGCTCTAAAGTTCACTGATCCTATACTTACAATGGGTAATGAATTGGTCCGGCGCATGAGATTGAGGAGCAAACACTTCATTGCTTTACATTTAAGGTATGAACCTGATATGCTTGCATTCTCGGGATGCTATTATGGTGGTGGTGATAAGGAAAGGAGAGAACTTGCAGCAATTAGAAGGAGATGGAAAACATTACATATTAACAACCCAGAGAAACAGAGGCGACAAGGAAGATGTCCACTCACTCCAGAAGAAGTCGGACTGATGCTTAGAGCTTTGGGATATGGCAGTGATGTTCATATATATGTCGCGTCTGGTGAAGTATATGGAGGAGAAGAATCATTGGCTCCTTTGAAAGCACTCTTTCCACACTTCTATTCAAAAGACACTATAGCTACAAAGGAAGAGTTAGAACCTTTTTCTTCGTACTCTTCTAGAATGGCTGCACTCGACTTCCTTGTTTGTGATGAAAGCGACGTATTTGTAACCAACAACAATGGCAATATGGCAAAAATATTGGCTGGGAGGAGGAGATACTTAGGACATAAACCCACTGTTAGGCCGAATGCAAAAAAGCTTTACAGATTATTCATGAACAAAGAGAACACGACATGGGAGGAGTTTTCATCTAAAGTCCGTTCGTTTCAGAGAGGGTTCATGGGAGAGCCAAAGGAAGTAAGAGCTGGTAGAGGTGAATTTCACGAAAACCCATCCACGTGCATATGCGAAGATACAGAGGCTAAGGCAAAGGCGCAATTAGAATCTAGAAAACTTGGAAAGAAAAACAAATCCACTAATAAAGACGCAGCAGTAACTGTAACCAATGATGATCAAACCGAAGAGGATGACCCAGATTGGTCGGAGCCAGACTATGAGGAAGAACAGAGTGATCTTCAGGACAGAGGATTATACAACGGGACAAGTTTAGATTACGATGATCCATCTACCTCTGATGAGCCTGAGCTTGAAGCAATGCTATCAGATTAG

Protein Analysis

572

Amino Acids

65.58

Weight (kDa)

9.27

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 109 - 424 1.4e-101 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
AatII GACGTC 1 cut(s) 519
AccII CGCG 2 cut(s) 153, 1043
AciI CCGC 3 cut(s) 16, 119, 392
AclWI GGATC 2 cut(s) 743, 1664
AcsI RAATTY 6 cut(s) 4, 254, 287, 295, 662, 1427
AcuI CTGAAG 1 cut(s) 1610
AcvI CACGTG 1 cut(s) 1449
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 4 cut(s) 556, 565, 582, 1160
AfiI CCNNNNNNNGG 4 cut(s) 125, 209, 545, 1290
AflIII ACRYGT 1 cut(s) 151
AgsI TTSAA 6 cut(s) 355, 653, 681, 1087, 1113, 1700
AhdI GACNNNNNGTC 1 cut(s) 439
AhlI ACTAGT 1 cut(s) 341
AjnI CCWGG 1 cut(s) 124
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 8 cut(s) 185, 209, 709, 1007, 1127, 1309, 1415, 1696
AluI AGCT 8 cut(s) 185, 209, 709, 1007, 1127, 1309, 1415, 1696
Alw26I GTCTC 4 cut(s) 44, 53, 76, 507
AlwI GGATC 2 cut(s) 743, 1664
AlwNI CAGNNNCTG 1 cut(s) 1547
Ama87I CYCGRG 1 cut(s) 853
AoxI GGCC 1 cut(s) 1292
ApeKI GCWGC 5 cut(s) 270, 282, 899, 1175, 1538
ApoI RAATTY 6 cut(s) 4, 254, 287, 295, 662, 1427
AspLEI GCGC 3 cut(s) 21, 786, 1486
AspS9I GGNCC 1 cut(s) 778
AsuHPI GGTGA 3 cut(s) 887, 1061, 1436
AsuII TTCGAA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 853
AvaII GGWCC 1 cut(s) 778
BbrPI CACGTG 1 cut(s) 1449
BbvI GCAGC 5 cut(s) 282, 294, 911, 1162, 1550
BccI CCATC 4 cut(s) 18, 909, 1450, 1681
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 1 cut(s) 126
BclI TGATCA 1 cut(s) 1561
BcoDI GTCTC 4 cut(s) 44, 53, 76, 507
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 3 cut(s) 342, 1167, 1497
BfmI CTRYAG 3 cut(s) 186, 670, 1122
BfoI RGCGCY 1 cut(s) 22
BisI GCNGC 7 cut(s) 17, 271, 283, 393, 900, 1176, 1539
BlsI GCNGC 7 cut(s) 18, 272, 284, 394, 901, 1177, 1540
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 1 cut(s) 778
BmeRI GACNNNNNGTC 1 cut(s) 439
BmeT110I CYCGRG 1 cut(s) 853
BmgT120I GGNCC 1 cut(s) 778
BmiI GGNNCC 2 cut(s) 1080, 1596
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 7 cut(s) 163, 257, 370, 445, 593, 848, 987
BpmI CTGGAG 4 cut(s) 147, 231, 531, 963
Bpu10I CCTNAGC 2 cut(s) 1473, 1692
Bpu14I TTCGAA 1 cut(s) 299
Bsa29I ATCGAT 1 cut(s) 322
BsaAI YACGTR 1 cut(s) 1449
BsaBI GATNNNNATC 1 cut(s) 135
BsaHI GRCGYC 1 cut(s) 516
BsaJI CCNNGG 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 209, 545, 1290
Bse1I ACTGG 3 cut(s) 373, 452, 459
Bse3DI GCAATG 3 cut(s) 280, 810, 1710
Bse8I GATNNNNATC 1 cut(s) 135
BseBI CCWGG 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 322
BseDI CCNNGG 1 cut(s) 584
BseGI GGATG 6 cut(s) 164, 272, 460, 863, 1442, 1582
BseJI GATNNNNATC 1 cut(s) 135
BseLI CCNNNNNNNGG 4 cut(s) 125, 209, 545, 1290
BseMI GCAATG 3 cut(s) 280, 810, 1710
BseMII CTCAG 1 cut(s) 1683
BseNI ACTGG 3 cut(s) 373, 452, 459
BseRI GAGGAG 6 cut(s) 74, 811, 1077, 1273, 1276, 1364
BseXI GCAGC 5 cut(s) 282, 294, 911, 1162, 1550
BseYI CCCAGC 1 cut(s) 1253
BsgI GTGCAG 1 cut(s) 1161
Bsh1236I CGCG 2 cut(s) 153, 1043
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 1 cut(s) 1294
BshVI ATCGAT 1 cut(s) 322
BsiEI CGRYCG 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 853
BsiSI CCGG 1 cut(s) 781
BslFI GGGAC 1 cut(s) 1663
BslI CCNNNNNNNGG 4 cut(s) 125, 209, 545, 1290
BsmAI GTCTC 4 cut(s) 44, 53, 76, 507
BsmBI CGTCTC 4 cut(s) 44, 53, 76, 507
BsmFI GGGAC 1 cut(s) 1663
BsmI GAATGC 3 cut(s) 579, 848, 1303
BsnI GGCC 1 cut(s) 1294
BsoBI CYCGRG 1 cut(s) 853
Bsp119I TTCGAA 1 cut(s) 299
Bsp143I GATC 9 cut(s) 136, 177, 218, 223, 319, 748, 1561, 1621, 1669
BspACI CCGC 3 cut(s) 16, 119, 392
BspANI GGCC 1 cut(s) 1294
BspCNI CTCAG 1 cut(s) 1684
BspDI ATCGAT 1 cut(s) 322
BspFNI CGCG 2 cut(s) 153, 1043
BspHI TCATGA 1 cut(s) 1323
BspLI GGNNCC 2 cut(s) 1080, 1596
BspPI GGATC 2 cut(s) 743, 1664
BspT104I TTCGAA 1 cut(s) 299
BsrDI GCAATG 3 cut(s) 280, 810, 1710
BsrI ACTGG 3 cut(s) 373, 452, 459
BssECI CCNNGG 1 cut(s) 584
BssMI GATC 9 cut(s) 136, 177, 218, 223, 319, 748, 1561, 1621, 1669
BssNI GRCGYC 1 cut(s) 516
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 4 cut(s) 38, 617, 1288, 1549
Bst6I CTCTTC 4 cut(s) 98, 1131, 1168, 1566
BstACI GRCGYC 1 cut(s) 516
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 1449
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 3 cut(s) 280, 645, 846
BstDEI CTNAG 5 cut(s) 710, 1001, 1270, 1473, 1692
BstDSI CCRYGG 1 cut(s) 584
BstF5I GGATG 6 cut(s) 164, 272, 460, 863, 1442, 1582
BstFNI CGCG 2 cut(s) 153, 1043
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 3 cut(s) 21, 786, 1486
BstKTI GATC 9 cut(s) 139, 180, 221, 226, 322, 751, 1564, 1624, 1672
BstMAI GTCTC 4 cut(s) 44, 53, 76, 507
BstMBI GATC 9 cut(s) 136, 177, 218, 223, 319, 748, 1561, 1621, 1669
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 3 cut(s) 182, 279, 389
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 1 cut(s) 647
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 3 cut(s) 186, 670, 1122
BstUI CGCG 2 cut(s) 153, 1043
BstV1I GCAGC 5 cut(s) 282, 294, 911, 1162, 1550
Bsu15I ATCGAT 1 cut(s) 322
BsuRI GGCC 1 cut(s) 1294
BsuTUI ATCGAT 1 cut(s) 322
BtgI CCRYGG 1 cut(s) 584
BtsCI GGATG 6 cut(s) 164, 272, 460, 863, 1442, 1582
BtsI GCAGTG 2 cut(s) 312, 1027
BtsIMutI CAGTG 6 cut(s) 312, 466, 539, 744, 1027, 1284
Cac8I GCNNGC 3 cut(s) 280, 645, 846
CaiI CAGNNNCTG 1 cut(s) 1547
CciI TCATGA 1 cut(s) 1323
CfoI GCGC 3 cut(s) 21, 786, 1486
Cfr13I GGNCC 1 cut(s) 778
ClaI ATCGAT 1 cut(s) 322
CseI GACGC 2 cut(s) 1032, 1544
Csp6I GTAC 4 cut(s) 555, 564, 581, 1159
CviAII CATG 7 cut(s) 558, 644, 731, 787, 1324, 1344, 1390
CviQI GTAC 4 cut(s) 555, 564, 581, 1159
DdeI CTNAG 5 cut(s) 710, 1001, 1270, 1473, 1692
DpnI GATC 9 cut(s) 138, 179, 220, 225, 321, 750, 1563, 1623, 1671
DpnII GATC 9 cut(s) 136, 177, 218, 223, 319, 748, 1561, 1621, 1669
DriI GACNNNNNGTC 1 cut(s) 439
Eam1104I CTCTTC 4 cut(s) 98, 1131, 1168, 1566
Eam1105I GACNNNNNGTC 1 cut(s) 439
EarI CTCTTC 4 cut(s) 98, 1131, 1168, 1566
EciI GGCGGA 1 cut(s) 108
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 778
Eco57I CTGAAG 1 cut(s) 1610
Eco72I CACGTG 1 cut(s) 1449
Eco88I CYCGRG 1 cut(s) 853
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 124
EcoRV GATATC 1 cut(s) 232
Esp3I CGTCTC 4 cut(s) 44, 53, 76, 507
FaeI CATG 7 cut(s) 561, 647, 734, 790, 1327, 1347, 1393
FaqI GGGAC 1 cut(s) 1663
FatI CATG 7 cut(s) 557, 643, 730, 786, 1323, 1343, 1389
FauNDI CATATG 1 cut(s) 1454
FbaI TGATCA 1 cut(s) 1561
Fnu4HI GCNGC 7 cut(s) 17, 271, 283, 393, 900, 1176, 1539
FokI GGATG 6 cut(s) 151, 279, 467, 870, 1429, 1589
Fsp4HI GCNGC 7 cut(s) 17, 271, 283, 393, 900, 1176, 1539
FspBI CTAG 3 cut(s) 342, 1167, 1497
GlaI GCGC 3 cut(s) 20, 785, 1485
GluI GCNGC 7 cut(s) 17, 271, 283, 393, 900, 1176, 1539
GsaI CCCAGC 1 cut(s) 1257
GsuI CTGGAG 4 cut(s) 147, 231, 531, 963
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 1 cut(s) 1294
HapII CCGG 1 cut(s) 781
HgaI GACGC 2 cut(s) 1032, 1544
HhaI GCGC 3 cut(s) 21, 786, 1486
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 7 cut(s) 561, 647, 734, 790, 1327, 1347, 1393
Hin6I GCGC 3 cut(s) 19, 784, 1484
HinP1I GCGC 3 cut(s) 19, 784, 1484
HindIII AAGCTT 1 cut(s) 1307
HinfI GANTC 4 cut(s) 131, 400, 1070, 1493
HpaII CCGG 1 cut(s) 781
HphI GGTGA 3 cut(s) 887, 1061, 1436
Hpy166II GTNNAC 4 cut(s) 242, 581, 744, 971
Hpy188I TCNGA 9 cut(s) 136, 204, 223, 521, 992, 1380, 1594, 1684, 1714
Hpy8I GTNNAC 4 cut(s) 242, 581, 744, 971
Hpy99I CGWCG 2 cut(s) 48, 1211
HpyAV CCTTC 4 cut(s) 62, 152, 445, 903
HpyCH4III ACNGT 4 cut(s) 38, 617, 1288, 1549
HpyCH4IV ACGT 3 cut(s) 516, 1209, 1448
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 279, 389
HpyF3I CTNAG 5 cut(s) 710, 1001, 1270, 1473, 1692
HpySE526I ACGT 3 cut(s) 516, 1209, 1448
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 7 cut(s) 561, 647, 734, 790, 1327, 1347, 1393
HspAI GCGC 3 cut(s) 19, 784, 1484
Ksp22I TGATCA 1 cut(s) 1561
Kzo9I GATC 9 cut(s) 136, 177, 218, 223, 319, 748, 1561, 1621, 1669
LmnI GCTCC 4 cut(s) 121, 798, 1084, 1594
Lsp1109I GCAGC 5 cut(s) 282, 294, 911, 1162, 1550
LweI GCATC 7 cut(s) 163, 257, 370, 445, 593, 848, 987
MaeI CTAG 3 cut(s) 342, 1167, 1497
MaeII ACGT 3 cut(s) 516, 1209, 1448
MaeIII GTNAC 6 cut(s) 307, 461, 593, 1216, 1543, 1549
MalI GATC 9 cut(s) 138, 179, 220, 225, 321, 750, 1563, 1623, 1671
MboI GATC 9 cut(s) 136, 177, 218, 223, 319, 748, 1561, 1621, 1669
MluI ACGCGT 1 cut(s) 151
MmeI TCCRAC 2 cut(s) 970, 1572
MseI TTAA 3 cut(s) 612, 824, 933
MslI CAYNNNNRTG 3 cut(s) 589, 1035, 1447
MspI CCGG 1 cut(s) 781
MspR9I CCNGG 1 cut(s) 126
Mva1269I GAATGC 3 cut(s) 579, 848, 1303
MvaI CCWGG 1 cut(s) 126
MvnI CGCG 2 cut(s) 153, 1043
MwoI GCNNNNNNNGC 3 cut(s) 182, 279, 389
NdeI CATATG 1 cut(s) 1454
NdeII GATC 9 cut(s) 136, 177, 218, 223, 319, 748, 1561, 1621, 1669
NlaIII CATG 7 cut(s) 561, 647, 734, 790, 1327, 1347, 1393
NlaIV GGNNCC 2 cut(s) 1080, 1596
NmuCI GTSAC 2 cut(s) 307, 461
NspI RCATGY 1 cut(s) 647
NspV TTCGAA 1 cut(s) 299
PaeI GCATGC 1 cut(s) 647
PagI TCATGA 1 cut(s) 1323
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 3 cut(s) 579, 848, 1303
PfeI GAWTC 4 cut(s) 131, 400, 1070, 1493
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 7 cut(s) 18, 272, 284, 394, 901, 1177, 1540
Ple19I CGATCG 1 cut(s) 139
PmaCI CACGTG 1 cut(s) 1449
PmlI CACGTG 1 cut(s) 1449
Ppu21I YACGTR 1 cut(s) 1449
PsiI TTATAA 1 cut(s) 527
Psp6I CCWGG 1 cut(s) 124
PspCI CACGTG 1 cut(s) 1449
PspFI CCCAGC 1 cut(s) 1253
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 1080, 1596
PspPI GGNCC 1 cut(s) 778
PstNI CAGNNNCTG 1 cut(s) 1547
PvuI CGATCG 1 cut(s) 139
RsaI GTAC 4 cut(s) 556, 565, 582, 1160
RsaNI GTAC 4 cut(s) 555, 564, 581, 1159
RseI CAYNNNNRTG 3 cut(s) 589, 1035, 1447
SaqAI TTAA 3 cut(s) 612, 824, 933
SatI GCNGC 7 cut(s) 17, 271, 283, 393, 900, 1176, 1539
Sau3AI GATC 9 cut(s) 136, 177, 218, 223, 319, 748, 1561, 1621, 1669
Sau96I GGNCC 1 cut(s) 778
ScrFI CCNGG 1 cut(s) 126
SfaNI GCATC 7 cut(s) 163, 257, 370, 445, 593, 848, 987
SfcI CTRYAG 3 cut(s) 186, 670, 1122
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 1 cut(s) 778
SmiMI CAYNNNNRTG 3 cut(s) 589, 1035, 1447
SpeI ACTAGT 1 cut(s) 341
SphI GCATGC 1 cut(s) 647
SsiI CCGC 3 cut(s) 16, 119, 392
SspI AATATT 1 cut(s) 1248
SspMI CTAG 3 cut(s) 342, 1167, 1497
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 4 cut(s) 38, 617, 1288, 1549
TaiI ACGT 3 cut(s) 519, 1212, 1451
TaqI TCGA 4 cut(s) 299, 322, 480, 1182
TatI WGTACW 1 cut(s) 563
TauI GCSGC 2 cut(s) 19, 395
TfiI GAWTC 4 cut(s) 131, 400, 1070, 1493
Tru1I TTAA 3 cut(s) 612, 824, 933
Tru9I TTAA 3 cut(s) 612, 824, 933
TscAI CASTG 6 cut(s) 312, 466, 546, 751, 1027, 1291
TseFI GTSAC 2 cut(s) 307, 461
TseI GCWGC 5 cut(s) 270, 282, 899, 1175, 1538
Tsp45I GTSAC 2 cut(s) 307, 461
TspGWI ACGGA 3 cut(s) 32, 601, 1358
TspRI CASTG 6 cut(s) 312, 466, 546, 751, 1027, 1291
VpaK11BI GGWCC 1 cut(s) 778
XapI RAATTY 6 cut(s) 4, 254, 287, 295, 662, 1427
XbaI TCTAGA 2 cut(s) 1166, 1496
XceI RCATGY 1 cut(s) 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
XspI CTAG 3 cut(s) 342, 1167, 1497
ZraI GACGTC 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.