MD15G1002400.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009884
Physical Location & Seq
Forward (+)
142930 .. 146474
3545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1002400.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGCTTTTCATTTCCAGCGCCGCCGCCTCCACTACTACCACCGCTTTCGCTTTCTTCTTCCCCTCATTTCGGCCATATCCGGAGCCCTTCTCGTCCTCTTCGCTCTTCTCTTGGTTCTTGCTCCTTCCCCTCTCGATGTCAATAACCTCCATCAACCGCGTCATTCTTCTACGTTCGATGTTGCCGTGGACGACGATGCCGTTTCTGCACGGCTTACTGTCTTTCGTGTTCCAAGCGGTGGAGGGAGATTGGATCGTGATATTTGGAGCTCCAGGAATTCCAAATTCTACTATGGATGCAGCAATGGCACCAAGAAATTTCCAAATGTTGATGAAATTACGCGTCCTAATAGGTACTTGGCGATTGCAACCAGTGGAGGCTTGAATCAACAAAGAACTGGGGGCATTGACGGAATCCGATTTGGAGAAGGAGGGGAAGAGCCAAGTGGTGCCTCCAGATATCAGCCAAATACGCTGGATCGACAATGCCCAACAACCACGATGTTGTCCCTGCGTGACTTCCTTCGAAGGCGGACAGGAATACAGGGTTGTACTCGGCTTTTGAGATGGCAGATTCCTCAGCTTCCTCTGCCCCTCTTCTCTGTACAACCCTGTGGAACGTGCCGTTTGGCCGCCATAACAGATGCTGTTGTAGCAGCTCGTATACTGAATGCTAGTCTTGTTGTTCCAAAGTTGGACCAGAAATCTTTCTGGAAGGATGCAAGTAACTTTTCAGAGATTTTTGATGTTGATTGGTTTATATCATTTCTGTCAAAAGATGTTAAAATCATAAAACAGCTTCCAAGGAAGGGAGTTAACATGTGGACGCCATCCACTATGCGTGTTCCAAGGAAGTGTAGTGAAAGATGTTATCAAAATCGTGTATTACCTGTTCTTTTGAAAAGGCATGCTATTCAGCTCAACAAGTTTGATTACAGACTTTCAAACAGGTTGAATACAGATCTGCAAAAATTAAGATGTAGAGTCAACTACCATGCTTTAAAGTTTACTGACCCAATACAGAAAATGGCCAAAAAGTTGGTTCATCAAATGAGGATGAGAAGCAAGCATTATATTGCATTGCACCTGAGGTATGAACCTGATATGCTTGCATTCTCAGGATGTTATTATGGTGGAGGAGACAAGGAAAGGAAAGAATTGGGTGCCATACAAAAGAGGTGGAAGACTTTACATGTAAGGATTAAGTGA

Protein Analysis

403

Amino Acids

46.24

Weight (kDa)

10.25

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 212 - 392 1.4e-56 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 308, 449, 1163
AccB7I CCANNNNNTGG 1 cut(s) 239
AccI GTMKAC 1 cut(s) 664
AccII CGCG 2 cut(s) 160, 343
AccIII TCCGGA 1 cut(s) 80
AciI CCGC 7 cut(s) 22, 25, 43, 158, 237, 532, 633
AclWI GGATC 2 cut(s) 261, 486
AcoI YGGCCR 3 cut(s) 72, 630, 1029
AcsI RAATTY 3 cut(s) 277, 284, 317
AcyI GRCGYC 1 cut(s) 827
AfaI GTAC 3 cut(s) 356, 553, 606
AfiI CCNNNNNNNGG 3 cut(s) 70, 239, 809
AflIII ACRYGT 3 cut(s) 341, 819, 1192
AgsI TTSAA 4 cut(s) 385, 901, 945, 955
AjnI CCWGG 1 cut(s) 272
AluBI AGCT 5 cut(s) 270, 583, 659, 799, 919
AluI AGCT 5 cut(s) 270, 583, 659, 799, 919
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 1 cut(s) 1134
AlwI GGATC 2 cut(s) 261, 486
AlwNI CAGNNNCTG 1 cut(s) 647
Aor13HI TCCGGA 1 cut(s) 80
AoxI GGCC 3 cut(s) 72, 630, 1029
ApeKI GCWGC 2 cut(s) 300, 656
ApoI RAATTY 3 cut(s) 277, 284, 317
Asp700I GAANNNNTTC 1 cut(s) 707
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 697
AsuII TTCGAA 1 cut(s) 526
AvaII GGWCC 1 cut(s) 697
AxyI CCTNAGG 1 cut(s) 1088
BalI TGGCCA 1 cut(s) 1031
BanI GGYRCC 3 cut(s) 308, 449, 1163
BanII GRGCYC 2 cut(s) 88, 272
BbsI GAAGAC 1 cut(s) 1190
Bbv12I GWGCWC 1 cut(s) 272
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 2 cut(s) 312, 668
BccI CCATC 3 cut(s) 159, 561, 838
BceAI ACGGC 4 cut(s) 170, 185, 227, 609
BciT130I CCWGG 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 1134
BfaI CTAG 1 cut(s) 675
BfoI RGCGCY 1 cut(s) 22
BglII AGATCT 1 cut(s) 961
BisI GCNGC 5 cut(s) 22, 25, 301, 633, 657
BlsI GCNGC 5 cut(s) 23, 26, 302, 634, 658
Bme1390I CCNGG 1 cut(s) 274
Bme18I GGWCC 1 cut(s) 697
BmgT120I GGNCC 1 cut(s) 697
BmiI GGNNCC 4 cut(s) 85, 310, 451, 1165
BmrFI CCNGG 1 cut(s) 274
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 4 cut(s) 187, 287, 634, 709
BmuI ACTGGG 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 1190
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpmI CTGGAG 2 cut(s) 256, 439
Bpu10I CCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 526
BsaHI GRCGYC 1 cut(s) 827
BsaJI CCNNGG 3 cut(s) 186, 803, 848
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 239, 809
Bse1I ACTGG 2 cut(s) 372, 403
Bse21I CCTNAGG 1 cut(s) 1088
Bse3DI GCAATG 2 cut(s) 310, 1079
BseAI TCCGGA 1 cut(s) 80
BseBI CCWGG 1 cut(s) 274
BseDI CCNNGG 3 cut(s) 186, 803, 848
BseGI GGATG 5 cut(s) 302, 724, 830, 1062, 1127
BseLI CCNNNNNNNGG 3 cut(s) 70, 239, 809
BseMI GCAATG 2 cut(s) 310, 1079
BseMII CTCAG 3 cut(s) 593, 1079, 1131
BseNI ACTGG 2 cut(s) 372, 403
BseRI GAGGAG 1 cut(s) 1152
BseXI GCAGC 2 cut(s) 312, 668
BsgI GTGCAG 1 cut(s) 192
Bsh1236I CGCG 2 cut(s) 160, 343
BshFI GGCC 3 cut(s) 74, 632, 1031
BshNI GGYRCC 3 cut(s) 308, 449, 1163
BsiHKAI GWGCWC 1 cut(s) 272
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 70, 239, 809
BsmAI GTCTC 1 cut(s) 1134
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 2 cut(s) 676, 1112
BsnI GGCC 3 cut(s) 74, 632, 1031
Bsp119I TTCGAA 1 cut(s) 526
Bsp1286I GDGCHC 2 cut(s) 88, 272
Bsp13I TCCGGA 1 cut(s) 80
Bsp1407I TGTACA 1 cut(s) 604
Bsp143I GATC 3 cut(s) 253, 478, 961
BspACI CCGC 7 cut(s) 22, 25, 43, 158, 237, 532, 633
BspANI GGCC 3 cut(s) 74, 632, 1031
BspCNI CTCAG 3 cut(s) 592, 1080, 1130
BspEI TCCGGA 1 cut(s) 80
BspFNI CGCG 2 cut(s) 160, 343
BspLI GGNNCC 4 cut(s) 85, 310, 451, 1165
BspPI GGATC 2 cut(s) 261, 486
BspQI GCTCTTC 2 cut(s) 111, 432
BspT104I TTCGAA 1 cut(s) 526
BspT107I GGYRCC 3 cut(s) 308, 449, 1163
BsrDI GCAATG 2 cut(s) 310, 1079
BsrGI TGTACA 1 cut(s) 604
BsrI ACTGG 2 cut(s) 372, 403
BssECI CCNNGG 3 cut(s) 186, 803, 848
BssMI GATC 3 cut(s) 253, 478, 961
BssNAI GTATAC 1 cut(s) 665
BssNI GRCGYC 1 cut(s) 827
BssT1I CCWWGG 2 cut(s) 803, 848
Bst1107I GTATAC 1 cut(s) 665
Bst2UI CCWGG 1 cut(s) 274
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 4 cut(s) 104, 111, 432, 602
BstACI GRCGYC 1 cut(s) 827
BstAUI TGTACA 1 cut(s) 604
BstBI TTCGAA 1 cut(s) 526
BstC8I GCNNGC 3 cut(s) 909, 1067, 1110
BstDEI CTNAG 3 cut(s) 579, 1088, 1117
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 5 cut(s) 302, 724, 830, 1062, 1127
BstFNI CGCG 2 cut(s) 160, 343
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 3 cut(s) 256, 481, 964
BstMAI GTCTC 1 cut(s) 1134
BstMBI GATC 3 cut(s) 253, 478, 961
BstMWI GCNNNNNNNGC 5 cut(s) 206, 306, 472, 589, 653
BstNI CCWGG 1 cut(s) 274
BstNSI RCATGY 3 cut(s) 823, 911, 1196
BstSCI CCNGG 1 cut(s) 272
BstUI CGCG 2 cut(s) 160, 343
BstV1I GCAGC 2 cut(s) 312, 668
BstV2I GAAGAC 1 cut(s) 1190
BstX2I RGATCY 1 cut(s) 961
BstXI CCANNNNNNTGG 1 cut(s) 1039
BstYI RGATCY 1 cut(s) 961
BstZ17I GTATAC 1 cut(s) 665
Bsu36I CCTNAGG 1 cut(s) 1088
BsuRI GGCC 3 cut(s) 74, 632, 1031
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 5 cut(s) 302, 724, 830, 1062, 1127
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 3 cut(s) 909, 1067, 1110
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 697
CseI GACGC 3 cut(s) 149, 332, 835
Csp6I GTAC 3 cut(s) 355, 552, 605
CspCI CAANNNNNGTGG 2 cut(s) 1160, 1195
CviAII CATG 4 cut(s) 820, 908, 995, 1193
CviQI GTAC 3 cut(s) 355, 552, 605
DdeI CTNAG 3 cut(s) 579, 1088, 1117
DpnI GATC 3 cut(s) 255, 480, 963
DpnII GATC 3 cut(s) 253, 478, 961
DraI TTTAAA 1 cut(s) 1002
EaeI YGGCCR 3 cut(s) 72, 630, 1029
Eam1104I CTCTTC 4 cut(s) 104, 111, 432, 602
EarI CTCTTC 4 cut(s) 104, 111, 432, 602
EciI GGCGGA 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 2 cut(s) 803, 848
Eco24I GRGCYC 2 cut(s) 88, 272
Eco32I GATATC 1 cut(s) 461
Eco47I GGWCC 1 cut(s) 697
Eco53kI GAGCTC 1 cut(s) 270
Eco81I CCTNAGG 1 cut(s) 1088
EcoICRI GAGCTC 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 272
EcoRV GATATC 1 cut(s) 461
EcoT14I CCWWGG 2 cut(s) 803, 848
EcoT38I GRGCYC 2 cut(s) 88, 272
ErhI CCWWGG 2 cut(s) 803, 848
FaeI CATG 4 cut(s) 823, 911, 998, 1196
FaqI GGGAC 1 cut(s) 493
FatI CATG 4 cut(s) 819, 907, 994, 1192
FblI GTMKAC 1 cut(s) 664
Fnu4HI GCNGC 5 cut(s) 22, 25, 301, 633, 657
FokI GGATG 5 cut(s) 309, 731, 817, 1069, 1134
FriOI GRGCYC 2 cut(s) 88, 272
Fsp4HI GCNGC 5 cut(s) 22, 25, 301, 633, 657
FspBI CTAG 1 cut(s) 675
GlaI GCGC 1 cut(s) 20
GluI GCNGC 5 cut(s) 22, 25, 301, 633, 657
GsuI CTGGAG 2 cut(s) 256, 439
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 3 cut(s) 74, 632, 1031
HapII CCGG 1 cut(s) 81
HgaI GACGC 3 cut(s) 149, 332, 835
HhaI GCGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 827
Hin1II CATG 4 cut(s) 823, 911, 998, 1196
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HincII GTYRAC 2 cut(s) 817, 988
HindII GTYRAC 2 cut(s) 817, 988
HinfI GANTC 4 cut(s) 385, 414, 574, 984
HpaI GTTAAC 1 cut(s) 817
HpaII CCGG 1 cut(s) 81
Hpy166II GTNNAC 6 cut(s) 190, 665, 817, 825, 988, 1008
Hpy188I TCNGA 2 cut(s) 419, 736
Hpy188III TCNNGA 6 cut(s) 81, 134, 257, 456, 712, 1119
Hpy8I GTNNAC 6 cut(s) 190, 665, 817, 825, 988, 1008
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 7 cut(s) 98, 135, 422, 522, 533, 709, 802
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 2 cut(s) 173, 620
HpyCH4V TGCA 8 cut(s) 209, 300, 368, 722, 967, 1079, 1084, 1112
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 306, 472, 589, 653
HpyF3I CTNAG 3 cut(s) 579, 1088, 1117
HpySE526I ACGT 2 cut(s) 173, 620
Hsp92I GRCGYC 1 cut(s) 827
Hsp92II CATG 4 cut(s) 823, 911, 998, 1196
HspAI GCGC 1 cut(s) 19
Kpn2I TCCGGA 1 cut(s) 80
KspAI GTTAAC 1 cut(s) 817
Kzo9I GATC 3 cut(s) 253, 478, 961
LguI GCTCTTC 2 cut(s) 111, 432
LmnI GCTCC 4 cut(s) 83, 127, 267, 275
Lsp1109I GCAGC 2 cut(s) 312, 668
LweI GCATC 4 cut(s) 187, 287, 634, 709
MaeI CTAG 1 cut(s) 675
MaeII ACGT 2 cut(s) 173, 620
MaeIII GTNAC 2 cut(s) 515, 725
MalI GATC 3 cut(s) 255, 480, 963
MboI GATC 3 cut(s) 253, 478, 961
MboII GAAGA 8 cut(s) 47, 50, 91, 98, 159, 449, 589, 1195
MflI RGATCY 1 cut(s) 961
MhlI GDGCHC 2 cut(s) 88, 272
MlsI TGGCCA 1 cut(s) 1031
MluCI AATT 6 cut(s) 277, 284, 317, 336, 971, 1157
MluI ACGCGT 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 1031
MlyI GAGTC 1 cut(s) 993
MmeI TCCRAC 1 cut(s) 675
Mox20I TGGCCA 1 cut(s) 1031
MroI TCCGGA 1 cut(s) 80
MroXI GAANNNNTTC 1 cut(s) 707
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 5 cut(s) 783, 816, 974, 1001, 1203
Msp20I TGGCCA 1 cut(s) 1031
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 274
Mva1269I GAATGC 2 cut(s) 676, 1112
MvaI CCWGG 1 cut(s) 274
MvnI CGCG 2 cut(s) 160, 343
MwoI GCNNNNNNNGC 5 cut(s) 206, 306, 472, 589, 653
NdeII GATC 3 cut(s) 253, 478, 961
NlaIII CATG 4 cut(s) 823, 911, 998, 1196
NlaIV GGNNCC 4 cut(s) 85, 310, 451, 1165
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 515
NspI RCATGY 3 cut(s) 823, 911, 1196
NspV TTCGAA 1 cut(s) 526
PaeI GCATGC 1 cut(s) 911
PciI ACATGT 2 cut(s) 819, 1192
PciSI GCTCTTC 2 cut(s) 111, 432
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 198
PctI GAATGC 2 cut(s) 676, 1112
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 3 cut(s) 385, 414, 574
PflMI CCANNNNNTGG 1 cut(s) 239
PfoI TCCNGGA 1 cut(s) 272
PkrI GCNGC 5 cut(s) 23, 26, 302, 634, 658
PleI GAGTC 1 cut(s) 992
PpsI GAGTC 1 cut(s) 992
PscI ACATGT 2 cut(s) 819, 1192
Psp124BI GAGCTC 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 272
PspGI CCWGG 1 cut(s) 272
PspN4I GGNNCC 4 cut(s) 85, 310, 451, 1165
PspPI GGNCC 1 cut(s) 697
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 1 cut(s) 961
RsaI GTAC 3 cut(s) 356, 553, 606
RsaNI GTAC 3 cut(s) 355, 552, 605
SacI GAGCTC 1 cut(s) 272
SapI GCTCTTC 2 cut(s) 111, 432
SaqAI TTAA 5 cut(s) 783, 816, 974, 1001, 1203
SatI GCNGC 5 cut(s) 22, 25, 301, 633, 657
Sau3AI GATC 3 cut(s) 253, 478, 961
Sau96I GGNCC 1 cut(s) 697
SchI GAGTC 1 cut(s) 993
ScrFI CCNGG 1 cut(s) 274
SduI GDGCHC 2 cut(s) 88, 272
SfaNI GCATC 4 cut(s) 187, 287, 634, 709
SfuI TTCGAA 1 cut(s) 526
SinI GGWCC 1 cut(s) 697
SphI GCATGC 1 cut(s) 911
Sse9I AATT 6 cut(s) 277, 284, 317, 336, 971, 1157
SsiI CCGC 7 cut(s) 22, 25, 43, 158, 237, 532, 633
SspMI CTAG 1 cut(s) 675
SstI GAGCTC 1 cut(s) 272
StyD4I CCNGG 1 cut(s) 272
StyI CCWWGG 2 cut(s) 803, 848
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 2 cut(s) 176, 623
TaqI TCGA 4 cut(s) 135, 177, 481, 526
TasI AATT 6 cut(s) 277, 284, 317, 336, 971, 1157
TatI WGTACW 2 cut(s) 551, 604
TauI GCSGC 3 cut(s) 24, 27, 635
TfiI GAWTC 3 cut(s) 385, 414, 574
Tru1I TTAA 5 cut(s) 783, 816, 974, 1001, 1203
Tru9I TTAA 5 cut(s) 783, 816, 974, 1001, 1203
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 2 cut(s) 300, 656
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 348, 1034, 1110
TspGWI ACGGA 1 cut(s) 426
TspRI CASTG 1 cut(s) 379
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 697
XapI RAATTY 3 cut(s) 277, 284, 317
XceI RCATGY 3 cut(s) 823, 911, 1196
XmiI GTMKAC 1 cut(s) 664
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.