Rh6CG455000

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009884
Physical Location & Seq
Reverse (-)
63062343 .. 63067684
5342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG455000.1

Sequence Viewer

Length: 1527 bp
ATGCTTGTTCATAACCAGAGCCGTGGCGGACGAAGATCCTCCGATCGTGATCTTTGGACTTCTAGGAATGCGAATCTCTACTATGGATGCAGCGATGCCAGCAAGAAGTTTCCGAAAGTTGAAGATGTCACGCATCCTAATAGGTATTTGGCAATTGCAACAAGTGGAGGCTTGAATCAACAAAGAACCGGGATTACAGATGCTGTTGTTGCTGCTCGCATACTGAATGCTAGTCTTGTTGTTCCAAAGTTGGACCAGAAGTCTTTCTGGAAGGATGCGAGTGAGTTTTCAGAAATCTTTGATGTTGATTATTTTATATCATATCTGTCGAAAGATGTTAAAATCATTAAGCAACTCCCAAGAAAGGGTGGTAAAACATGGACTCCATACAATATGCGTGTCCCAAGGAAGTGTAGTGAAAAATGTTATCAGACTCGTCTAGTACCTGTTCTTTTGAAAAAGCATGCTGTTCAGCTCACAAAGTTTGATTACAGACTTGCAAACAGGTTGGGTACAGATTTGCAAAAATTGAGATGCAGAGTGAATTATCATGCTTTAAAGTTTACTGAGCCAATACGGAAAATGGGTGAAAAGTTGGTTCATCGAATGAGGATGAAAAGCAAGCACTATATCACATTGCACCTGAGGTTTGAACCTGATATGCTTGCATTCTCTGGATGCTATTATGGTGGAGGAGACAGAGAAAGGAAAGAACTTGGTGCAATACGGAAGAGGTGGAAGACGTTACATATAAGCAACCCTGATAAGCCAAGGAGGCATGGGAGATGTCCCCTTACTCCAGAAGAAGTTGGTCTGATGTTGAGAGCACTTGGTTATGGCAGTGATGTTCATATTTATGTGGCATCAGGGGAAGTGTATGGTGGGGAAGACACGTTGGCGCCCCTTAAAGCACTATTTCCCAACTTCTATTCAAAAGAGACAATAGCAACCAAAGAGGAGTTGGAACCATTCTATTCATTTTCTTCTCGCATGGCTGCTCTTGATTTTATAGTTTGTGATGAAAGTGATGTTTTTGTCACTAACAACAATGGTAATATGGCCAAAATATTAGCTGGACGAAGGAGATATTTTGGGCATAAGCCTACTATTCGTCCAAATGCTAAAAAACTTTATCGATTGTTCTTGAGCAAAGAGAACACAACTTGGGAAGCTTTTGCATCTAGTGTTCGTACTTACCAGAGAGGCTTTATGGGTGAGCCACATGAAGGGAGGCCAGGCAGGGGTGGGTTTCATGAGAACCCATCCACCTGCATATGTGAAAATTCTCAGGCTAAAGTCAAGAGAGATGTGGAACCTAGAAAATACGGCAAGGGTAGTAATGTAACAAGAAAAGATGAAGTAAAAGTCGATGTCCAAGACTCGGAAGATGATCCAGAATGGCCCGACTCTGATGAGGATGAAGATCTAAGTGTCCCGCTGGAGAATGATCACTTTAACAGAACTACAGAGGATTATGATACAGTAAACTCAGAGGAACCTGAATTGGAGGAGCTTATTTCTGACTAG

Protein Analysis

508

Amino Acids

58.35

Weight (kDa)

8.99

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 49 - 363 4.9e-102 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1277
Acc36I ACCTGC 1 cut(s) 1277
AccB1I GGYRCC 1 cut(s) 898
AciI CCGC 2 cut(s) 27, 1436
AclWI GGATC 2 cut(s) 30, 1385
AcoI YGGCCR 1 cut(s) 1059
AcsI RAATTY 1 cut(s) 1282
AcyI GRCGYC 1 cut(s) 899
AfaI GTAC 3 cut(s) 444, 514, 1192
AfiI CCNNNNNNNGG 5 cut(s) 364, 365, 1226, 1241, 1381
AflIII ACRYGT 1 cut(s) 891
AgsI TTSAA 5 cut(s) 122, 175, 457, 653, 933
AhdI GACNNNNNGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 1234
AluBI AGCT 4 cut(s) 475, 1073, 1172, 1513
AluI AGCT 4 cut(s) 475, 1073, 1172, 1513
Alw21I GWGCWC 1 cut(s) 829
Alw26I GTCTC 2 cut(s) 690, 932
AlwI GGATC 2 cut(s) 30, 1385
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 3 cut(s) 1059, 1232, 1400
ApeKI GCWGC 3 cut(s) 90, 212, 995
ApoI RAATTY 1 cut(s) 1282
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 2 cut(s) 253, 1401
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 2 cut(s) 599, 1226
AvaII GGWCC 1 cut(s) 253
AxyI CCTNAGG 1 cut(s) 644
BalI TGGCCA 1 cut(s) 1061
BanI GGYRCC 1 cut(s) 898
BbsI GAAGAC 2 cut(s) 746, 894
Bbv12I GWGCWC 1 cut(s) 829
BbvI GCAGC 3 cut(s) 102, 199, 982
BccI CCATC 1 cut(s) 1270
BceAI ACGGC 2 cut(s) 6, 1342
BciT130I CCWGG 1 cut(s) 1236
BclI TGATCA 1 cut(s) 1447
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 690, 932
BfaI CTAG 6 cut(s) 63, 231, 440, 1182, 1317, 1525
BfmI CTRYAG 1 cut(s) 1464
BfoI RGCGCY 1 cut(s) 902
BfuAI ACCTGC 1 cut(s) 1277
BglI GCCNNNNNGGC 1 cut(s) 775
BglII AGATCT 1 cut(s) 1423
BisI GCNGC 3 cut(s) 91, 213, 996
BlsI GCNGC 3 cut(s) 92, 214, 997
Bme1390I CCNGG 2 cut(s) 190, 1236
Bme18I GGWCC 1 cut(s) 253
BmeRI GACNNNNNGTC 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 253, 1401
BmiI GGNNCC 4 cut(s) 900, 966, 1314, 1497
BmrFI CCNGG 2 cut(s) 190, 1236
BmsI GCATC 9 cut(s) 77, 85, 142, 190, 265, 524, 668, 872, 1187
BpiI GAAGAC 2 cut(s) 746, 894
BpmI CTGGAG 2 cut(s) 783, 1460
BpuEI CTTGAG 1 cut(s) 1165
BpuMI CCSGG 1 cut(s) 190
Bsa29I ATCGAT 1 cut(s) 1135
BsaBI GATNNNNATC 2 cut(s) 48, 1422
BsaHI GRCGYC 1 cut(s) 899
BsaJI CCNNGG 3 cut(s) 22, 404, 770
BsaXI ACNNNNNCTCC 2 cut(s) 367, 397
Bsc4I CCNNNNNNNGG 5 cut(s) 364, 365, 1226, 1241, 1381
Bse21I CCTNAGG 1 cut(s) 644
Bse3DI GCAATG 1 cut(s) 635
Bse8I GATNNNNATC 2 cut(s) 48, 1422
BseBI CCWGG 1 cut(s) 1236
BseCI ATCGAT 1 cut(s) 1135
BseDI CCNNGG 3 cut(s) 22, 404, 770
BseGI GGATG 7 cut(s) 92, 133, 280, 618, 683, 1262, 1423
BseJI GATNNNNATC 2 cut(s) 48, 1422
BseLI CCNNNNNNNGG 5 cut(s) 364, 365, 1226, 1241, 1381
BseMI GCAATG 1 cut(s) 635
BseMII CTCAG 4 cut(s) 558, 635, 1301, 1503
BseRI GAGGAG 3 cut(s) 708, 971, 1523
BseXI GCAGC 3 cut(s) 102, 199, 982
Bsh1285I CGRYCG 1 cut(s) 46
BshFI GGCC 3 cut(s) 1061, 1234, 1402
BshNI GGYRCC 1 cut(s) 898
BshVI ATCGAT 1 cut(s) 1135
BsiEI CGRYCG 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 829
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 3 cut(s) 386, 774, 1418
BslI CCNNNNNNNGG 5 cut(s) 364, 365, 1226, 1241, 1381
BsmAI GTCTC 2 cut(s) 690, 932
BsmFI GGGAC 3 cut(s) 386, 774, 1418
BsmI GAATGC 3 cut(s) 73, 232, 668
BsnI GGCC 3 cut(s) 1061, 1234, 1402
Bsp1286I GDGCHC 1 cut(s) 829
Bsp143I GATC 6 cut(s) 35, 43, 49, 1390, 1423, 1447
BspACI CCGC 2 cut(s) 27, 1436
BspANI GGCC 3 cut(s) 1061, 1234, 1402
BspCNI CTCAG 4 cut(s) 559, 636, 1300, 1502
BspDI ATCGAT 1 cut(s) 1135
BspHI TCATGA 1 cut(s) 1252
BspLI GGNNCC 4 cut(s) 900, 966, 1314, 1497
BspMI ACCTGC 1 cut(s) 1277
BspPI GGATC 2 cut(s) 30, 1385
BspT107I GGYRCC 1 cut(s) 898
BsrDI GCAATG 1 cut(s) 635
BssECI CCNNGG 3 cut(s) 22, 404, 770
BssMI GATC 6 cut(s) 35, 43, 49, 1390, 1423, 1447
BssNI GRCGYC 1 cut(s) 899
BssT1I CCWWGG 2 cut(s) 404, 770
Bst2UI CCWGG 1 cut(s) 1236
Bst4CI ACNGT 1 cut(s) 1483
Bst6I CTCTTC 1 cut(s) 725
BstACI GRCGYC 1 cut(s) 899
BstC8I GCNNGC 5 cut(s) 100, 217, 465, 623, 666
BstDEI CTNAG 5 cut(s) 567, 644, 1287, 1427, 1489
BstDSI CCRYGG 1 cut(s) 22
BstF5I GGATG 7 cut(s) 92, 133, 280, 618, 683, 1262, 1423
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 1 cut(s) 901
BstKTI GATC 6 cut(s) 38, 46, 52, 1393, 1426, 1450
BstMAI GTCTC 2 cut(s) 690, 932
BstMBI GATC 6 cut(s) 35, 43, 49, 1390, 1423, 1447
BstMCI CGRYCG 1 cut(s) 46
BstMWI GCNNNNNNNGC 3 cut(s) 99, 209, 775
BstNI CCWGG 1 cut(s) 1236
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 2 cut(s) 188, 1234
BstSFI CTRYAG 1 cut(s) 1464
BstV1I GCAGC 3 cut(s) 102, 199, 982
BstV2I GAAGAC 2 cut(s) 746, 894
BstX2I RGATCY 2 cut(s) 35, 1423
BstXI CCANNNNNNTGG 1 cut(s) 23
BstYI RGATCY 2 cut(s) 35, 1423
Bsu15I ATCGAT 1 cut(s) 1135
Bsu36I CCTNAGG 1 cut(s) 644
BsuRI GGCC 3 cut(s) 1061, 1234, 1402
BsuTUI ATCGAT 1 cut(s) 1135
BtgI CCRYGG 1 cut(s) 22
BtgZI GCGATG 1 cut(s) 108
BtsCI GGATG 7 cut(s) 92, 133, 280, 618, 683, 1262, 1423
BtsI GCAGTG 1 cut(s) 847
BtsIMutI CAGTG 1 cut(s) 847
BveI ACCTGC 1 cut(s) 1277
Cac8I GCNNGC 5 cut(s) 100, 217, 465, 623, 666
CaiI CAGNNNCTG 1 cut(s) 203
CciI TCATGA 1 cut(s) 1252
CfoI GCGC 1 cut(s) 901
Cfr13I GGNCC 2 cut(s) 253, 1401
ClaI ATCGAT 1 cut(s) 1135
Csp6I GTAC 3 cut(s) 443, 513, 1191
CviAII CATG 7 cut(s) 378, 464, 551, 779, 991, 1223, 1253
CviQI GTAC 3 cut(s) 443, 513, 1191
DdeI CTNAG 5 cut(s) 567, 644, 1287, 1427, 1489
DinI GGCGCC 1 cut(s) 900
DpnI GATC 6 cut(s) 37, 45, 51, 1392, 1425, 1449
DpnII GATC 6 cut(s) 35, 43, 49, 1390, 1423, 1447
DraI TTTAAA 1 cut(s) 558
DriI GACNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 1059
Eam1104I CTCTTC 1 cut(s) 725
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 725
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 404, 770
Eco47I GGWCC 1 cut(s) 253
Eco81I CCTNAGG 1 cut(s) 644
EcoRII CCWGG 1 cut(s) 1234
EcoT14I CCWWGG 2 cut(s) 404, 770
EgeI GGCGCC 1 cut(s) 900
EheI GGCGCC 1 cut(s) 900
ErhI CCWWGG 2 cut(s) 404, 770
FaeI CATG 7 cut(s) 381, 467, 554, 782, 994, 1226, 1256
FaqI GGGAC 3 cut(s) 386, 774, 1418
FatI CATG 7 cut(s) 377, 463, 550, 778, 990, 1222, 1252
FauI CCCGC 1 cut(s) 1443
FauNDI CATATG 1 cut(s) 1274
FbaI TGATCA 1 cut(s) 1447
Fnu4HI GCNGC 3 cut(s) 91, 213, 996
FokI GGATG 7 cut(s) 99, 120, 287, 625, 690, 1249, 1430
Fsp4HI GCNGC 3 cut(s) 91, 213, 996
FspBI CTAG 6 cut(s) 63, 231, 440, 1182, 1317, 1525
GlaI GCGC 1 cut(s) 900
GluI GCNGC 3 cut(s) 91, 213, 996
GsuI CTGGAG 2 cut(s) 783, 1460
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 3 cut(s) 1061, 1234, 1402
HapII CCGG 1 cut(s) 189
HhaI GCGC 1 cut(s) 901
Hin1I GRCGYC 1 cut(s) 899
Hin1II CATG 7 cut(s) 381, 467, 554, 782, 994, 1226, 1256
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HindIII AAGCTT 1 cut(s) 1170
HinfI GANTC 6 cut(s) 73, 175, 382, 433, 1379, 1406
HpaII CCGG 1 cut(s) 189
HphI GGTGA 2 cut(s) 599, 1226
Hpy166II GTNNAC 2 cut(s) 564, 1486
Hpy188I TCNGA 9 cut(s) 43, 114, 292, 432, 816, 1384, 1411, 1492, 1522
Hpy188III TCNNGA 9 cut(s) 47, 268, 675, 800, 1001, 1144, 1253, 1300, 1394
Hpy8I GTNNAC 2 cut(s) 564, 1486
HpyAV CCTTC 3 cut(s) 265, 1074, 1220
HpyCH4III ACNGT 1 cut(s) 1483
HpyCH4IV ACGT 2 cut(s) 743, 893
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 209, 775
HpyF3I CTNAG 5 cut(s) 567, 644, 1287, 1427, 1489
HpySE526I ACGT 2 cut(s) 743, 893
Hsp92I GRCGYC 1 cut(s) 899
Hsp92II CATG 7 cut(s) 381, 467, 554, 782, 994, 1226, 1256
HspAI GCGC 1 cut(s) 899
KasI GGCGCC 1 cut(s) 898
Ksp22I TGATCA 1 cut(s) 1447
Kzo9I GATC 6 cut(s) 35, 43, 49, 1390, 1423, 1447
LmnI GCTCC 1 cut(s) 1510
Lsp1109I GCAGC 3 cut(s) 102, 199, 982
LweI GCATC 9 cut(s) 77, 85, 142, 190, 265, 524, 668, 872, 1187
MaeI CTAG 6 cut(s) 63, 231, 440, 1182, 1317, 1525
MaeII ACGT 2 cut(s) 743, 893
MaeIII GTNAC 4 cut(s) 127, 744, 1036, 1342
MalI GATC 6 cut(s) 37, 45, 51, 1392, 1425, 1449
MboI GATC 6 cut(s) 35, 43, 49, 1390, 1423, 1447
MboII GAAGA 9 cut(s) 45, 134, 742, 751, 815, 899, 975, 1397, 1433
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 2 cut(s) 35, 1423
MhlI GDGCHC 1 cut(s) 829
MlsI TGGCCA 1 cut(s) 1061
MluCI AATT 5 cut(s) 153, 527, 544, 1282, 1502
MluNI TGGCCA 1 cut(s) 1061
Mly113I GGCGCC 1 cut(s) 899
MlyI GAGTC 4 cut(s) 376, 427, 1373, 1400
MmeI TCCRAC 2 cut(s) 231, 942
Mox20I TGGCCA 1 cut(s) 1061
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 1061
MseI TTAA 5 cut(s) 339, 348, 557, 906, 1455
MslI CAYNNNNRTG 1 cut(s) 855
Msp20I TGGCCA 1 cut(s) 1061
MspA1I CMGCKG 1 cut(s) 1438
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 2 cut(s) 190, 1236
MunI CAATTG 1 cut(s) 153
Mva1269I GAATGC 3 cut(s) 73, 232, 668
MvaI CCWGG 1 cut(s) 1236
MwoI GCNNNNNNNGC 3 cut(s) 99, 209, 775
NarI GGCGCC 1 cut(s) 899
NciI CCSGG 1 cut(s) 190
NdeI CATATG 1 cut(s) 1274
NdeII GATC 6 cut(s) 35, 43, 49, 1390, 1423, 1447
NlaIII CATG 7 cut(s) 381, 467, 554, 782, 994, 1226, 1256
NlaIV GGNNCC 4 cut(s) 900, 966, 1314, 1497
NmuCI GTSAC 2 cut(s) 127, 1036
NspI RCATGY 1 cut(s) 467
PaeI GCATGC 1 cut(s) 467
PagI TCATGA 1 cut(s) 1252
PaqCI CACCTGC 1 cut(s) 1277
PctI GAATGC 3 cut(s) 73, 232, 668
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 73, 175
PkrI GCNGC 3 cut(s) 92, 214, 997
Ple19I CGATCG 1 cut(s) 46
PleI GAGTC 4 cut(s) 376, 427, 1373, 1400
PluTI GGCGCC 1 cut(s) 902
PpsI GAGTC 4 cut(s) 376, 427, 1373, 1400
Psp6I CCWGG 1 cut(s) 1234
PspGI CCWGG 1 cut(s) 1234
PspN4I GGNNCC 4 cut(s) 900, 966, 1314, 1497
PspPI GGNCC 2 cut(s) 253, 1401
PsrI GAACNNNNNNTAC 2 cut(s) 178, 210
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 2 cut(s) 35, 1423
PvuI CGATCG 1 cut(s) 46
RsaI GTAC 3 cut(s) 444, 514, 1192
RsaNI GTAC 3 cut(s) 443, 513, 1191
RseI CAYNNNNRTG 1 cut(s) 855
SaqAI TTAA 5 cut(s) 339, 348, 557, 906, 1455
SatI GCNGC 3 cut(s) 91, 213, 996
Sau3AI GATC 6 cut(s) 35, 43, 49, 1390, 1423, 1447
Sau96I GGNCC 2 cut(s) 253, 1401
SchI GAGTC 4 cut(s) 376, 427, 1373, 1400
ScrFI CCNGG 2 cut(s) 190, 1236
SduI GDGCHC 1 cut(s) 829
SfaNI GCATC 9 cut(s) 77, 85, 142, 190, 265, 524, 668, 872, 1187
SfcI CTRYAG 1 cut(s) 1464
SfoI GGCGCC 1 cut(s) 900
SinI GGWCC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 855
SmlI CTYRAG 1 cut(s) 1144
SmoI CTYRAG 1 cut(s) 1144
SphI GCATGC 1 cut(s) 467
Sse9I AATT 5 cut(s) 153, 527, 544, 1282, 1502
SsiI CCGC 2 cut(s) 27, 1436
SspDI GGCGCC 1 cut(s) 898
SspI AATATT 1 cut(s) 1068
SspMI CTAG 6 cut(s) 63, 231, 440, 1182, 1317, 1525
StyD4I CCNGG 2 cut(s) 188, 1234
StyI CCWWGG 2 cut(s) 404, 770
TaaI ACNGT 1 cut(s) 1483
TaiI ACGT 2 cut(s) 746, 896
TaqI TCGA 4 cut(s) 329, 604, 1135, 1368
TasI AATT 5 cut(s) 153, 527, 544, 1282, 1502
TfiI GAWTC 2 cut(s) 73, 175
Tru1I TTAA 5 cut(s) 339, 348, 557, 906, 1455
Tru9I TTAA 5 cut(s) 339, 348, 557, 906, 1455
TscAI CASTG 1 cut(s) 847
TseFI GTSAC 2 cut(s) 127, 1036
TseI GCWGC 3 cut(s) 90, 212, 995
Tsp45I GTSAC 2 cut(s) 127, 1036
TspDTI ATGAA 9 cut(s) 590, 629, 839, 966, 1035, 1239, 1241, 1371, 1434
TspGWI ACGGA 2 cut(s) 592, 742
TspRI CASTG 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 253
XapI RAATTY 1 cut(s) 1282
XceI RCATGY 1 cut(s) 467
XcmI CCANNNNNNNNNTGG 1 cut(s) 958
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 6 cut(s) 63, 231, 440, 1182, 1317, 1525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.