AT2G14620

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0016123
Physical Location & Seq
Forward (+)
6244772 .. 6246279
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G14620.1

Sequence Viewer

Length: 900 bp
ATGACTCTAATAAATCGCTCAAAACCATTTGTACTTCTCGTAGGCTTCTCAATTATCTCAAGTTTGCTATTATGGGTCTCACAAGCTTCCGTTGTTTCATCAGGAGATTTTAACAAAGACTTCTTTGTGACATGGTCTCCTACCCACGTTAACACCTCCAATGATGGTCGATCAAGAACCCTTAAACTCGATCAAGAATCTGGAGCTTCTTTTTCTTCCATTCAGACGTTCTTGTTCGGCCAAATAGATATGAAGATCAAACTTATCCGTGGAAGTTCTCAAGGAACCGTGGTTGCTTATTATATGTCCTCAGATCAGCCTAACCGGGATGAGATTGACTTTGAGTTCCTCGGAAACGTTAATGGGCAGCCTTACATTCTCCAGACAAATGTCTATGCCGAGGGACTTGATAACCGCGAAGAAAGGATCCATCTCTGGTTCGACCCAGCCAAGGACTTTCACACTTACTCTATCTTGTGGAACATTCACCAAATTGTATTTATGGTGGATCAGATTCCGATAAGGTTGTATAGAAACCATGGGGAGAAAGGCGTAGCCTACCCAAGGTTGCAACCTATGAGTGTACAGGCGAGTCTATGGAACGGTGAGAGCTGGGCTACGCGCGGTGGACATGACAAGATTGATTGGTCAAAGGGTCCATTTGTGGCTTCCTTTGGGGATTACAAGATAGACGCTTGTATTTGGATAGGCAATACAAGTTTTTGTAATGGAGAGAGCACTGAAAACTGGTGGAACAAGAATGAGTTCAGTTCTTTGACAAGAGTGCAAAAGAGATGGTTTAAATGGGTGAGAAAATATCACTTGATTTATGATTATTGCCAAGATTATGGAAGGTTCAATAACAAGCTACCGAAGGAATGTTCTCTTCCTAAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

299

Amino Acids

34.69

Weight (kDa)

8.24

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 38 - 218 2.7e-61 Glycosyl hydrolases family 16
XET_C PF06955 246 - 294 4.2e-16 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14620
fragaria_vesca FvH4_2g02970
malus_domestica MD10G1112700.v1.1
prunus_persica Prupe.8G152500_v2.0.a1
pyrus_communis pycom10g09750
rosa_chinensis RchiOBHm_Chr6g0247771
rosa_laevigata RLG00000015181
rosa_multiflora Rmu_sc0004057.1_g000004 Rmu_sc0009569.1_g000006
rosa_roxburghii Rroxscaffold_180G00433260 Rroxscaffold_7G00213680
rosa_rugosa Rorug05G0528400
rosa_samantha Rh6BG038100 Rh6DG033900
rosa_wichuraiana Rw6G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 417, 622, 624
AciI CCGC 2 cut(s) 415, 624
AclI AACGTT 1 cut(s) 357
AclWI GGATC 3 cut(s) 421, 434, 516
AcoI YGGCCR 1 cut(s) 238
AfaI GTAC 2 cut(s) 33, 585
AfiI CCNNNNNNNGG 2 cut(s) 451, 564
AgsI TTSAA 1 cut(s) 859
AluBI AGCT 4 cut(s) 86, 206, 612, 868
AluI AGCT 4 cut(s) 86, 206, 612, 868
Alw21I GWGCWC 1 cut(s) 740
Alw26I GTCTC 2 cut(s) 82, 141
AlwI GGATC 3 cut(s) 421, 434, 516
AoxI GGCC 1 cut(s) 238
ApeKI GCWGC 1 cut(s) 367
Asp700I GAANNNNTTC 1 cut(s) 764
AspLEI GCGC 1 cut(s) 624
AspS9I GGNCC 1 cut(s) 656
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 3 cut(s) 479, 617, 820
AvaII GGWCC 1 cut(s) 656
BamHI GGATCC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 740
BbvI GCAGC 1 cut(s) 379
BccI CCATC 3 cut(s) 158, 438, 789
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 2 cut(s) 82, 141
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 3 cut(s) 286, 428, 657
BmrFI CCNGG 1 cut(s) 326
BoxI GACNNNNGTC 1 cut(s) 389
BpmI CTGGAG 2 cut(s) 222, 365
BpuEI CTTGAG 2 cut(s) 43, 264
BpuMI CCSGG 1 cut(s) 326
BsaI GGTCTC 2 cut(s) 82, 141
BsaJI CCNNGG 7 cut(s) 268, 288, 349, 399, 450, 538, 563
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 2 cut(s) 451, 564
Bse1I ACTGG 1 cut(s) 752
BseDI CCNNGG 7 cut(s) 268, 288, 349, 399, 450, 538, 563
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 2 cut(s) 451, 564
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 752
BseXI GCAGC 1 cut(s) 379
BseYI CCCAGC 2 cut(s) 445, 612
Bsh1236I CGCG 3 cut(s) 417, 622, 624
BshFI GGCC 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 740
BsiSI CCGG 1 cut(s) 325
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 2 cut(s) 451, 564
BsmAI GTCTC 2 cut(s) 82, 141
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 1 cut(s) 240
Bso31I GGTCTC 2 cut(s) 82, 141
Bsp1286I GDGCHC 1 cut(s) 740
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 6 cut(s) 170, 190, 255, 313, 426, 508
Bsp19I CCATGG 1 cut(s) 538
BspACI CCGC 2 cut(s) 415, 624
BspANI GGCC 1 cut(s) 240
BspCNI CTCAG 1 cut(s) 323
BspFNI CGCG 3 cut(s) 417, 622, 624
BspLI GGNNCC 3 cut(s) 286, 428, 657
BspPI GGATC 3 cut(s) 421, 434, 516
BspTNI GGTCTC 2 cut(s) 82, 141
BsrGI TGTACA 1 cut(s) 583
BsrI ACTGG 1 cut(s) 752
BssECI CCNNGG 7 cut(s) 268, 288, 349, 399, 450, 538, 563
BssMI GATC 6 cut(s) 170, 190, 255, 313, 426, 508
BssT1I CCWWGG 3 cut(s) 450, 538, 563
Bst4CI ACNGT 2 cut(s) 289, 605
Bst6I CTCTTC 1 cut(s) 891
BstAUI TGTACA 1 cut(s) 583
BstDEI CTNAG 1 cut(s) 310
BstDSI CCRYGG 3 cut(s) 268, 288, 538
BstENI CCTNNNNNAGG 1 cut(s) 562
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 3 cut(s) 417, 622, 624
BstHHI GCGC 1 cut(s) 624
BstKTI GATC 6 cut(s) 173, 193, 258, 316, 429, 511
BstMAI GTCTC 2 cut(s) 82, 141
BstMBI GATC 6 cut(s) 170, 190, 255, 313, 426, 508
BstPAI GACNNNNGTC 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 324
BstUI CGCG 3 cut(s) 417, 622, 624
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 426
BstXI CCANNNNNNTGG 1 cut(s) 848
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 1 cut(s) 240
BtgI CCRYGG 3 cut(s) 268, 288, 538
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 738
CfoI GCGC 1 cut(s) 624
Cfr13I GGNCC 1 cut(s) 656
CseI GACGC 1 cut(s) 701
Csp6I GTAC 2 cut(s) 32, 584
CviAII CATG 3 cut(s) 132, 539, 632
CviQI GTAC 2 cut(s) 32, 584
DdeI CTNAG 1 cut(s) 310
DpnI GATC 6 cut(s) 172, 192, 257, 315, 428, 510
DpnII GATC 6 cut(s) 170, 190, 255, 313, 426, 508
DraI TTTAAA 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 891
Eco130I CCWWGG 3 cut(s) 450, 538, 563
Eco31I GGTCTC 2 cut(s) 82, 141
Eco47I GGWCC 1 cut(s) 656
EcoNI CCTNNNNNAGG 1 cut(s) 562
EcoT14I CCWWGG 3 cut(s) 450, 538, 563
ErhI CCWWGG 3 cut(s) 450, 538, 563
FaeI CATG 3 cut(s) 135, 542, 635
FaqI GGGAC 1 cut(s) 417
FatI CATG 3 cut(s) 131, 538, 631
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 368
GlaI GCGC 1 cut(s) 623
GluI GCNGC 1 cut(s) 368
GsaI CCCAGC 2 cut(s) 449, 616
GsuI CTGGAG 2 cut(s) 222, 365
HaeIII GGCC 1 cut(s) 240
HapII CCGG 1 cut(s) 325
HgaI GACGC 1 cut(s) 701
HhaI GCGC 1 cut(s) 624
Hin1II CATG 3 cut(s) 135, 542, 635
Hin6I GCGC 1 cut(s) 622
HinP1I GCGC 1 cut(s) 622
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 4 cut(s) 4, 197, 514, 592
HpaI GTTAAC 1 cut(s) 151
HpaII CCGG 1 cut(s) 325
HphI GGTGA 3 cut(s) 479, 617, 820
Hpy166II GTNNAC 3 cut(s) 151, 584, 629
Hpy188I TCNGA 5 cut(s) 225, 313, 353, 513, 519
Hpy188III TCNNGA 5 cut(s) 102, 174, 194, 201, 382
Hpy8I GTNNAC 3 cut(s) 151, 584, 629
HpyAV CCTTC 2 cut(s) 846, 868
HpyCH4III ACNGT 2 cut(s) 289, 605
HpyCH4IV ACGT 3 cut(s) 147, 227, 357
HpyCH4V TGCA 2 cut(s) 571, 787
HpyF3I CTNAG 1 cut(s) 310
HpySE526I ACGT 3 cut(s) 147, 227, 357
Hsp92II CATG 3 cut(s) 135, 542, 635
HspAI GCGC 1 cut(s) 622
KspAI GTTAAC 1 cut(s) 151
Kzo9I GATC 6 cut(s) 170, 190, 255, 313, 426, 508
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 9 cut(s) 87, 186, 338, 395, 421, 459, 572, 598, 733
Lsp1109I GCAGC 1 cut(s) 379
MaeII ACGT 3 cut(s) 147, 227, 357
MaeIII GTNAC 1 cut(s) 127
MalI GATC 6 cut(s) 172, 192, 257, 315, 428, 510
MboI GATC 6 cut(s) 170, 190, 255, 313, 426, 508
MboII GAAGA 4 cut(s) 207, 265, 431, 878
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 740
MluCI AATT 2 cut(s) 51, 492
MlyI GAGTC 1 cut(s) 601
MnlI CCTC 4 cut(s) 166, 319, 359, 394
MroXI GAANNNNTTC 1 cut(s) 764
MseI TTAA 5 cut(s) 111, 150, 183, 360, 801
MspI CCGG 1 cut(s) 325
MspR9I CCNGG 1 cut(s) 326
MvnI CGCG 3 cut(s) 417, 622, 624
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 1 cut(s) 538
NdeII GATC 6 cut(s) 170, 190, 255, 313, 426, 508
NlaIII CATG 3 cut(s) 135, 542, 635
NlaIV GGNNCC 3 cut(s) 286, 428, 657
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 764
PfeI GAWTC 2 cut(s) 197, 514
PflFI GACNNNGTC 1 cut(s) 133
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 1 cut(s) 600
PpsI GAGTC 1 cut(s) 600
PshAI GACNNNNGTC 1 cut(s) 389
Psp1406I AACGTT 1 cut(s) 357
PspFI CCCAGC 2 cut(s) 445, 612
PspN4I GGNNCC 3 cut(s) 286, 428, 657
PspPI GGNCC 1 cut(s) 656
PsuI RGATCY 1 cut(s) 426
PsyI GACNNNGTC 1 cut(s) 133
RsaI GTAC 2 cut(s) 33, 585
RsaNI GTAC 2 cut(s) 32, 584
SaqAI TTAA 5 cut(s) 111, 150, 183, 360, 801
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 6 cut(s) 170, 190, 255, 313, 426, 508
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 1 cut(s) 740
SinI GGWCC 1 cut(s) 656
SmlI CTYRAG 2 cut(s) 58, 279
SmoI CTYRAG 2 cut(s) 58, 279
Sse9I AATT 2 cut(s) 51, 492
SsiI CCGC 2 cut(s) 415, 624
SspI AATATT 1 cut(s) 896
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 3 cut(s) 450, 538, 563
TaaI ACNGT 2 cut(s) 289, 605
TaiI ACGT 3 cut(s) 150, 230, 360
TaqI TCGA 3 cut(s) 169, 189, 441
TasI AATT 2 cut(s) 51, 492
TatI WGTACW 2 cut(s) 31, 583
TfiI GAWTC 2 cut(s) 197, 514
Tru1I TTAA 5 cut(s) 111, 150, 183, 360, 801
Tru9I TTAA 5 cut(s) 111, 150, 183, 360, 801
TscAI CASTG 1 cut(s) 745
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 87, 266
TspGWI ACGGA 2 cut(s) 79, 257
TspRI CASTG 1 cut(s) 745
Tth111I GACNNNGTC 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 656
XagI CCTNNNNNAGG 1 cut(s) 562
XmnI GAANNNNTTC 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.