Rorug05G0528400

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016123
Physical Location & Seq
Reverse (-)
71760805 .. 71767686
6882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0528400.1

Sequence Viewer

Length: 2988 bp
ATGGGTCGCTGCAGTTTCTCCTCGGACGATATCGTAATAAAGTCTCCGAATGATAAGAAACTGTACAGATTAATCAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGATCCTGAGATTTACCCACAAGGGCCCAAAAGCCTTGAACCCAGTGAAGAAGATGAAGCAGCCAAAGGTGGAGATGGTGCCTCTGAGACTAAGACGGCAGCAGCAGCAATGTGCGTTGGAATAGGCAGCATGTCTGACCCTGTTGAGGCACAAGGGCTTGCACACTTCCTAGAACACATGCTCTTTATGGGGAGTACAAAGTTTCCTATTGAAAATGAGTTTGGTAGTTACTTGTCCAAGCATGGAGGATGGTCAAATGCATACACAGAAGCAGAGCAGACTTGCTACTATTTTGTTGTAAAACGAGAGTTTCTTAAGGGTGCCTTATCAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGTGAAGCCATGGAGCGAGAGTTACAGGCTATAGATTCAGAATTTAACAGGGTTCTGCAAAACGATTTTTGCCGCCTTCAGCAACTTCAATGCTATACATCATCACCTGGTCACCCATTTAACAAATTCTCTTGGGGAAATAAGAAGAGCTTGGTTAATGCAAAAGAAAAAGGGATCGACTTGCGTGAACAAATAATGAAATTTTACAGTGACTATTACCATGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATGTACTTGAGGAGTGGATTTTAGAACTGTATGGAGATGTTAAAAGTGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGAAGGTCCTATATGGAAATCTGGAAAACTTTACAGGCTAGAGGCTGTTGACAATGTTCATATACTCCACTTAGCATGGACACTTCCATGTCTTCATCGACACTATTTGAAAAGCCCAACATATTATTTACGTCATCTCCTTGAGCATGAGGGCAGGGGAAGTTTGTATTTCTACCTCAAAGCTAGAGGTTGGGCAACATTTGTAAGTGCTCCAGTGGAACACTATTCTGTGGCTGATGTCTTCTGCATGATCATATACCTCACTGACTCTGGATTGGAGAAGATTTTCGATATAATTGGGTTGGTGTATCAATACATTAAGTTCTTACGGCAAGTGTTGCCCCAAGAATGGATATTTCGTGAACTCCAGGATATTGGGAACATCTACCTTAGGTTTTTAGAGGAGCAGCCCCAGGATTATTATACTTTAAGACTTGGAGGAAATTTACTACATTATCCCGCAGAGCATGCTATTTATGGGGACTATTTGCTAGAGAGTTGGGATGAGAAATTGATAGAATATACTCTAGGTTTCTTGAGACCAGAAAACATGAGGATTGATGTGATATCTAAGTCCTCCACGTTGTCAGAAGATTTCCAGTGCGAGCCTTGGTTTGGGTCACATTTGACCGAGGAAGATATATCTCCGTCTTTGATAGATTTATGGAAGGATCCTCAAGAAATTGATAGTTCATTGCATCTCCCAGAAAAGAATGAATTCATTCCCTCTGATTTTTCCATCCGTTCTGATGTTCTTGATACAGCAAATACATCTTGTCCTAGATGTATACTTGATGGGCCATTGGTGAAGTTATGGTACAAGCTTGATAGTACTTACAAACTTCCGCAGGCAAACATATATTTTCGCGTCAGTCTAAAGGGAGCATCTGGTAGTGTGAAGAGTTCTGTCTTGACTCAACTATATGGCGACCTTCTTTGGGATGAGCTGAATGAGGTTGTATATCAGGCTAGTGTCGCCCAGCTGGGAACTTCTGTATCTGTGTACACTAACTATCTGGAGCTGAAGGTCAGCGGTTTCAATGATAAGATTCCAACTCTCTTGTCAAAAATTATGACAACAGCCAAAAATTTCTCGCCAACTTACGAATGTTTCAAGGTTATTAAAGAGGACATGGAGCGAGCATATAAGAACACCAATATGGATCCTTGGAGCCACTCGACATACTTGAGAAATGAAGTTTTGTACCAAAATTTCTGTGATGTAGATGAGACGTTGCATGTTTTAAATGGATTGACTGTTTCTGATGTGAAGTCATTCATTCCCGAGCTTTGGTCCCCGCATTACATTGAAGGCCTTTGCCATGGAAATCTGTTAGAAGAAGAAGCAATCAGCCTTTTAAATATAGTGAAGACAAATTTTTCTGTGCAACCACATCCTATCGAACTGAAGCATAAAAATAACTGTATTTGTCTTCCTCCTGATGCTAACGTCATTAGAGATGTCAGAGTGAAGAATATGTCTCAAACAAACTCTGTTATTGAGCTGTATTTTCAAATTGAGTGGGCAGTGGGGATTGAGTCCACCAGATTGAGAGCATTGATAGATCTCTTTTGTGAAATTGTAAACGAACCACTTTACAATCAGCTAAGGACAAAGGAGCAGCTGGGATATGTTGTTCAATGTAACTGGAATGTAACACTCGATGTTTTTGGCTTTTATTTTAAAGTTCAGTCGTCTGATTACAATCCAATCTACTTACAAAGAAGAGTTGACAACTTTATCAATGATATGGAAGAGCTTTTGCTAGGATTGGATGATGAATCCTTTGAGAATTACCAAGGTGGGTTAATAGCGAAGCTATTGGAGAAAGATTCAACCCTTATGGATGAAACTGCTCGATATTGGGATCATATTATCTGCAAAAGGTATAAGTTTGACAAATGGGAAAAGATAGCAGAAGAACTCAGAAGTCTACAGAAGGGGGATGTTATCAACTTTTACAAGACTTACTTGCAACTATCATCTCCCAAGCGTCGGAGACTTGTAACTCGTGTTTGGGGTTGCAACACTGACATGAAAGAAGCTGAAGAAGCGCGAACGGAGTCTGAGCAAGTCATTGAAGACCTTGCAGCCTTTAAGAGGTCATCTCAGTTCTATGGTCATGGAAGTTGTCAGACAGTTGCTCCTCCGATTATGTGTAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

995

Amino Acids

114.64

Weight (kDa)

5.24

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 66 - 181 4.2e-31 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 221 - 396 8.4e-18 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 404 - 686 1.4e-80 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 691 - 873 1.5e-17 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 796 - 895 2.3e-24 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14620
fragaria_vesca FvH4_2g02970
malus_domestica MD10G1112700.v1.1
prunus_persica Prupe.8G152500_v2.0.a1
pyrus_communis pycom10g09750
rosa_chinensis RchiOBHm_Chr6g0247771
rosa_laevigata RLG00000015181
rosa_multiflora Rmu_sc0004057.1_g000004 Rmu_sc0009569.1_g000006
rosa_roxburghii Rroxscaffold_180G00433260 Rroxscaffold_7G00213680
rosa_rugosa Rorug05G0528400
rosa_samantha Rh6BG038100 Rh6DG033900
rosa_wichuraiana Rw6G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 188, 431
AccI GTMKAC 2 cut(s) 1606, 2752
AccII CGCG 2 cut(s) 1686, 2875
AciI CCGC 5 cut(s) 550, 1278, 1664, 1851, 2117
AclWI GGATC 7 cut(s) 107, 659, 1484, 1497, 1976, 1989, 2694
AcsI RAATTY 8 cut(s) 518, 602, 677, 1261, 1535, 1906, 2029, 2194
AcuI CTGAAG 4 cut(s) 539, 1862, 2246, 2886
AfaI GTAC 7 cut(s) 65, 307, 747, 1637, 1651, 1823, 2024
AfiI CCNNNNNNNGG 7 cut(s) 256, 1167, 1433, 1756, 2109, 2814, 2919
AflII CTTAAG 1 cut(s) 425
AjnI CCWGG 3 cut(s) 583, 1185, 1230
AjuI GAANNNNNNNTTGG 2 cut(s) 315, 347
AloI GAACNNNNNNTCC 2 cut(s) 2439, 2471
Alw21I GWGCWC 1 cut(s) 1030
Alw26I GTCTC 6 cut(s) 48, 191, 1351, 2042, 2304, 2812
AlwI GGATC 7 cut(s) 107, 659, 1484, 1497, 1976, 1989, 2694
Ama87I CYCGRG 1 cut(s) 2102
AoxI GGCC 4 cut(s) 88, 134, 1616, 2131
ApaI GGGCCC 1 cut(s) 138
ApeKI GCWGC 9 cut(s) 9, 170, 209, 212, 215, 237, 1225, 2440, 2909
ApoI RAATTY 8 cut(s) 518, 602, 677, 1261, 1535, 1906, 2029, 2194
AseI ATTAAT 2 cut(s) 71, 707
Asp700I GAANNNNTTC 4 cut(s) 1103, 1535, 1539, 2093
AspLEI GCGC 1 cut(s) 2875
AspS9I GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
AsuHPI GGTGA 4 cut(s) 456, 573, 581, 1636
AvaI CYCGRG 1 cut(s) 2102
AvaII GGWCC 3 cut(s) 791, 824, 2112
AxyI CCTNAGG 1 cut(s) 1208
BaeGI GKGCMC 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 1797, 1830
BamHI GGATCC 2 cut(s) 1489, 1981
BanI GGYRCC 2 cut(s) 188, 431
BanII GRGCYC 1 cut(s) 138
BarI GAAGNNNNNNTAC 2 cut(s) 1619, 1651
BauI CACGAG 1 cut(s) 2829
BbsI GAAGAC 5 cut(s) 902, 1051, 2195, 2243, 2907
Bbv12I GWGCWC 1 cut(s) 1030
BbvI GCAGC 8 cut(s) 182, 221, 224, 227, 249, 1237, 2452, 2921
BccI CCATC 4 cut(s) 179, 354, 1565, 1607
BceAI ACGGC 2 cut(s) 222, 1163
BciT130I CCWGG 3 cut(s) 585, 1187, 1232
BclI TGATCA 1 cut(s) 1068
BcoDI GTCTC 6 cut(s) 48, 191, 1351, 2042, 2304, 2812
BfaI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
BfmI CTRYAG 3 cut(s) 10, 507, 2753
BfrI CTTAAG 1 cut(s) 425
BglII AGATCT 1 cut(s) 2383
BmcAI AGTACT 1 cut(s) 1651
Bme1390I CCNGG 3 cut(s) 585, 1187, 1232
Bme18I GGWCC 3 cut(s) 791, 824, 2112
BmeT110I CYCGRG 1 cut(s) 2102
BmgT120I GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
BmiI GGNNCC 8 cut(s) 136, 190, 433, 793, 1491, 1983, 1991, 2114
BmrFI CCNGG 3 cut(s) 585, 1187, 1232
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 3 cut(s) 1525, 1712, 2251
BmuI ACTGGG 1 cut(s) 147
BpiI GAAGAC 5 cut(s) 902, 1051, 2195, 2243, 2907
BplI GAGNNNNNCTC 2 cut(s) 2911, 2943
BpmI CTGGAG 4 cut(s) 827, 1014, 1169, 1856
Bpu10I CCTNAGC 1 cut(s) 2426
BpuEI CTTGAG 6 cut(s) 101, 770, 980, 1375, 1479, 2026
BsaBI GATNNNNATC 1 cut(s) 2523
BsaI GGTCTC 1 cut(s) 1351
BsaJI CCNNGG 9 cut(s) 21, 486, 697, 1230, 1427, 1449, 1985, 2140, 2617
BsaXI ACNNNNNCTCC 6 cut(s) 482, 512, 939, 969, 2947, 2977
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 1167, 1433, 1756, 2109, 2814, 2919
Bse1I ACTGG 4 cut(s) 153, 1031, 1417, 2471
Bse21I CCTNAGG 1 cut(s) 1208
Bse3DI GCAATG 3 cut(s) 98, 225, 1511
Bse8I GATNNNNATC 1 cut(s) 2523
BseBI CCWGG 3 cut(s) 585, 1187, 1232
BseDI CCNNGG 9 cut(s) 21, 486, 697, 1230, 1427, 1449, 1985, 2140, 2617
BseGI GGATG 8 cut(s) 365, 1327, 1557, 1765, 2212, 2599, 2671, 2770
BseJI GATNNNNATC 1 cut(s) 2523
BseLI CCNNNNNNNGG 7 cut(s) 256, 1167, 1433, 1756, 2109, 2814, 2919
BseMI GCAATG 3 cut(s) 98, 225, 1511
BseMII CTCAG 6 cut(s) 108, 186, 464, 2758, 2877, 2942
BseNI ACTGG 4 cut(s) 153, 1031, 1417, 2471
BseRI GAGGAG 4 cut(s) 10, 767, 1235, 2955
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 8 cut(s) 182, 221, 224, 227, 249, 1237, 2452, 2921
BseYI CCCAGC 3 cut(s) 1797, 1801, 2443
Bsh1236I CGCG 2 cut(s) 1686, 2875
BshFI GGCC 4 cut(s) 90, 136, 1618, 2133
BshNI GGYRCC 2 cut(s) 188, 431
BsiHKAI GWGCWC 1 cut(s) 1030
BsiHKCI CYCGRG 1 cut(s) 2102
BslFI GGGAC 3 cut(s) 777, 1313, 2098
BslI CCNNNNNNNGG 7 cut(s) 256, 1167, 1433, 1756, 2109, 2814, 2919
BsmAI GTCTC 6 cut(s) 48, 191, 1351, 2042, 2304, 2812
BsmBI CGTCTC 1 cut(s) 2042
BsmFI GGGAC 3 cut(s) 777, 1313, 2098
BsnI GGCC 4 cut(s) 90, 136, 1618, 2133
Bso31I GGTCTC 1 cut(s) 1351
BsoBI CYCGRG 1 cut(s) 2102
Bsp120I GGGCCC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 138, 1030
Bsp1407I TGTACA 2 cut(s) 63, 1821
Bsp143I GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
Bsp19I CCATGG 3 cut(s) 486, 697, 2140
BspACI CCGC 5 cut(s) 550, 1278, 1664, 1851, 2117
BspANI GGCC 4 cut(s) 90, 136, 1618, 2133
BspCNI CTCAG 6 cut(s) 109, 187, 463, 2757, 2878, 2941
BspFNI CGCG 2 cut(s) 1686, 2875
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 8 cut(s) 136, 190, 433, 793, 1491, 1983, 1991, 2114
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 7 cut(s) 107, 659, 1484, 1497, 1976, 1989, 2694
BspQI GCTCTTC 2 cut(s) 617, 2568
BspT107I GGYRCC 2 cut(s) 188, 431
BspTI CTTAAG 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 1351
BsrDI GCAATG 3 cut(s) 98, 225, 1511
BsrGI TGTACA 2 cut(s) 63, 1821
BsrI ACTGG 4 cut(s) 153, 1031, 1417, 2471
BssECI CCNNGG 9 cut(s) 21, 486, 697, 1230, 1427, 1449, 1985, 2140, 2617
BssMI GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
BssNAI GTATAC 1 cut(s) 1607
BssSI CACGAG 1 cut(s) 2829
BssT1I CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
Bst1107I GTATAC 1 cut(s) 1607
Bst2BI CACGAG 1 cut(s) 2829
Bst2UI CCWGG 3 cut(s) 585, 1187, 1232
Bst4CI ACNGT 6 cut(s) 63, 686, 771, 2077, 2243, 2959
Bst6I CTCTTC 4 cut(s) 617, 1712, 2539, 2568
BstAFI CTTAAG 1 cut(s) 425
BstAPI GCANNNNNTGC 2 cut(s) 1156, 1286
BstAUI TGTACA 2 cut(s) 63, 1821
BstC8I GCNNGC 5 cut(s) 270, 1287, 1424, 1668, 1959
BstDSI CCRYGG 3 cut(s) 486, 697, 2140
BstEII GGTNACC 1 cut(s) 587
BstENI CCTNNNNNAGG 1 cut(s) 2917
BstF5I GGATG 8 cut(s) 365, 1327, 1557, 1765, 2212, 2599, 2671, 2770
BstFNI CGCG 2 cut(s) 1686, 2875
BstHHI GCGC 1 cut(s) 2875
BstKTI GATC 7 cut(s) 115, 654, 1071, 1492, 1984, 2386, 2689
BstMAI GTCTC 6 cut(s) 48, 191, 1351, 2042, 2304, 2812
BstMBI GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
BstMWI GCNNNNNNNGC 5 cut(s) 215, 1156, 1286, 1793, 2870
BstNI CCWGG 3 cut(s) 585, 1187, 1232
BstNSI RCATGY 4 cut(s) 244, 292, 1289, 2060
BstPI GGTNACC 1 cut(s) 587
BstSCI CCNGG 3 cut(s) 583, 1185, 1230
BstSFI CTRYAG 3 cut(s) 10, 507, 2753
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 2 cut(s) 1686, 2875
BstV1I GCAGC 8 cut(s) 182, 221, 224, 227, 249, 1237, 2452, 2921
BstV2I GAAGAC 5 cut(s) 902, 1051, 2195, 2243, 2907
BstX2I RGATCY 3 cut(s) 1489, 1981, 2383
BstXI CCANNNNNNTGG 1 cut(s) 1193
BstYI RGATCY 3 cut(s) 1489, 1981, 2383
BstZ17I GTATAC 1 cut(s) 1607
Bsu36I CCTNAGG 1 cut(s) 1208
BsuRI GGCC 4 cut(s) 90, 136, 1618, 2133
BtgI CCRYGG 3 cut(s) 486, 697, 2140
BtsCI GGATG 8 cut(s) 365, 1327, 1557, 1765, 2212, 2599, 2671, 2770
BtsI GCAGTG 2 cut(s) 93, 2352
BtsIMutI CAGTG 8 cut(s) 93, 160, 691, 1038, 1080, 1424, 2352, 2847
Cac8I GCNNGC 5 cut(s) 270, 1287, 1424, 1668, 1959
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 2875
Cfr13I GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
CseI GACGC 2 cut(s) 1675, 2801
CsiI ACCWGGT 1 cut(s) 583
Csp6I GTAC 7 cut(s) 64, 306, 746, 1636, 1650, 1822, 2023
CspCI CAANNNNNGTGG 2 cut(s) 2321, 2356
CviQI GTAC 7 cut(s) 64, 306, 746, 1636, 1650, 1822, 2023
DpnI GATC 7 cut(s) 114, 653, 1070, 1491, 1983, 2385, 2688
DpnII GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
DraI TTTAAA 3 cut(s) 2064, 2178, 2503
Eam1104I CTCTTC 4 cut(s) 617, 1712, 2539, 2568
EarI CTCTTC 4 cut(s) 617, 1712, 2539, 2568
Eco130I CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
Eco147I AGGCCT 1 cut(s) 2133
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 1351
Eco32I GATATC 2 cut(s) 31, 1386
Eco47I GGWCC 3 cut(s) 791, 824, 2112
Eco57I CTGAAG 4 cut(s) 539, 1862, 2246, 2886
Eco81I CCTNAGG 1 cut(s) 1208
Eco88I CYCGRG 1 cut(s) 2102
Eco91I GGTNACC 1 cut(s) 587
EcoNI CCTNNNNNAGG 1 cut(s) 2917
EcoO109I RGGNCCY 2 cut(s) 134, 824
EcoO65I GGTNACC 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 1535
EcoRII CCWGG 3 cut(s) 583, 1185, 1230
EcoRV GATATC 2 cut(s) 31, 1386
EcoT14I CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
Esp3I CGTCTC 1 cut(s) 2042
FalI AAGNNNNNCTT 6 cut(s) 419, 451, 611, 643, 2774, 2806
FaqI GGGAC 3 cut(s) 777, 1313, 2098
FauI CCCGC 2 cut(s) 1285, 2124
FbaI TGATCA 1 cut(s) 1068
FblI GTMKAC 2 cut(s) 1606, 2752
FokI GGATG 8 cut(s) 372, 1334, 1544, 1772, 2199, 2606, 2678, 2777
FriOI GRGCYC 1 cut(s) 138
FspBI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
GlaI GCGC 1 cut(s) 2874
GsaI CCCAGC 3 cut(s) 1801, 1805, 2447
GsuI CTGGAG 4 cut(s) 827, 1014, 1169, 1856
HaeIII GGCC 4 cut(s) 90, 136, 1618, 2133
HgaI GACGC 2 cut(s) 1675, 2801
HhaI GCGC 1 cut(s) 2875
Hin6I GCGC 1 cut(s) 2873
HinP1I GCGC 1 cut(s) 2873
HincII GTYRAC 2 cut(s) 868, 2551
HindII GTYRAC 2 cut(s) 868, 2551
HindIII AAGCTT 3 cut(s) 77, 1640, 2981
HinfI GANTC 9 cut(s) 512, 734, 1085, 1732, 1867, 2357, 2600, 2651, 2882
HphI GGTGA 4 cut(s) 456, 573, 581, 1636
Hpy99I CGWCG 1 cut(s) 2817
HpyAV CCTTC 7 cut(s) 563, 815, 1480, 1760, 1837, 2123, 2752
HpyCH4III ACNGT 6 cut(s) 63, 686, 771, 2077, 2243, 2959
HpyCH4IV ACGT 4 cut(s) 949, 1400, 2051, 2268
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 1156, 1286, 1793, 2870
HpySE526I ACGT 4 cut(s) 949, 1400, 2051, 2268
HspAI GCGC 1 cut(s) 2873
Ksp22I TGATCA 1 cut(s) 1068
Kzo9I GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
LguI GCTCTTC 2 cut(s) 617, 2568
LmnI GCTCC 9 cut(s) 490, 1033, 1222, 1700, 1837, 1954, 1989, 2437, 2968
Lsp1109I GCAGC 8 cut(s) 182, 221, 224, 227, 249, 1237, 2452, 2921
LweI GCATC 3 cut(s) 1525, 1712, 2251
MabI ACCWGGT 1 cut(s) 583
MaeI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
MaeII ACGT 4 cut(s) 949, 1400, 2051, 2268
MaeIII GTNAC 8 cut(s) 338, 498, 587, 686, 1437, 2462, 2473, 2824
MalI GATC 7 cut(s) 114, 653, 1070, 1491, 1983, 2385, 2688
MboI GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
MflI RGATCY 3 cut(s) 1489, 1981, 2383
MhlI GDGCHC 2 cut(s) 138, 1030
MlyI GAGTC 4 cut(s) 1079, 1726, 2366, 2891
MmeI TCCRAC 3 cut(s) 208, 1895, 2795
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 4 cut(s) 1103, 1535, 1539, 2093
MslI CAYNNNNRTG 3 cut(s) 904, 1976, 2852
MspA1I CMGCKG 3 cut(s) 1801, 1851, 2443
MspCI CTTAAG 1 cut(s) 425
MspR9I CCNGG 3 cut(s) 585, 1187, 1232
MvaI CCWGG 3 cut(s) 585, 1187, 1232
MvnI CGCG 2 cut(s) 1686, 2875
MwoI GCNNNNNNNGC 5 cut(s) 215, 1156, 1286, 1793, 2870
NcoI CCATGG 3 cut(s) 486, 697, 2140
NdeII GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
NlaIV GGNNCC 8 cut(s) 136, 190, 433, 793, 1491, 1983, 1991, 2114
NmuCI GTSAC 3 cut(s) 587, 686, 1437
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 4 cut(s) 244, 292, 1289, 2060
PaeI GCATGC 1 cut(s) 1289
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 2133
PciSI GCTCTTC 2 cut(s) 617, 2568
PdmI GAANNNNTTC 4 cut(s) 1103, 1535, 1539, 2093
PfeI GAWTC 5 cut(s) 512, 734, 1867, 2600, 2651
PfoI TCCNGGA 1 cut(s) 1185
PleI GAGTC 4 cut(s) 1079, 1726, 2365, 2890
PpsI GAGTC 4 cut(s) 1079, 1726, 2365, 2890
PpuMI RGGWCCY 1 cut(s) 824
PshBI ATTAAT 2 cut(s) 71, 707
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 3 cut(s) 583, 1185, 1230
PspEI GGTNACC 1 cut(s) 587
PspFI CCCAGC 3 cut(s) 1797, 1801, 2443
PspGI CCWGG 3 cut(s) 583, 1185, 1230
PspN4I GGNNCC 8 cut(s) 136, 190, 433, 793, 1491, 1983, 1991, 2114
PspOMI GGGCCC 1 cut(s) 134
PspPI GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
PspPPI RGGWCCY 1 cut(s) 824
PstI CTGCAG 1 cut(s) 14
PsuI RGATCY 3 cut(s) 1489, 1981, 2383
PvuII CAGCTG 2 cut(s) 1801, 2443
RsaI GTAC 7 cut(s) 65, 307, 747, 1637, 1651, 1823, 2024
RsaNI GTAC 7 cut(s) 64, 306, 746, 1636, 1650, 1822, 2023
RseI CAYNNNNRTG 3 cut(s) 904, 1976, 2852
SapI GCTCTTC 2 cut(s) 617, 2568
Sau3AI GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
Sau96I GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
ScaI AGTACT 1 cut(s) 1651
SchI GAGTC 4 cut(s) 1079, 1726, 2366, 2891
ScrFI CCNGG 3 cut(s) 585, 1187, 1232
SduI GDGCHC 2 cut(s) 138, 1030
SexAI ACCWGGT 1 cut(s) 583
SfaNI GCATC 3 cut(s) 1525, 1712, 2251
SfcI CTRYAG 3 cut(s) 10, 507, 2753
SinI GGWCC 3 cut(s) 791, 824, 2112
SmiMI CAYNNNNRTG 3 cut(s) 904, 1976, 2852
SmlI CTYRAG 7 cut(s) 80, 425, 749, 959, 1354, 1494, 2005
SmoI CTYRAG 7 cut(s) 80, 425, 749, 959, 1354, 1494, 2005
SphI GCATGC 1 cut(s) 1289
SseBI AGGCCT 1 cut(s) 2133
SsiI CCGC 5 cut(s) 550, 1278, 1664, 1851, 2117
SspMI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
StuI AGGCCT 1 cut(s) 2133
StyD4I CCNGG 3 cut(s) 583, 1185, 1230
StyI CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
TaaI ACNGT 6 cut(s) 63, 686, 771, 2077, 2243, 2959
TaiI ACGT 4 cut(s) 952, 1403, 2054, 2271
TaqI TCGA 7 cut(s) 654, 916, 1107, 1997, 2220, 2481, 2677
TaqII GACCGA 1 cut(s) 1463
TatI WGTACW 5 cut(s) 63, 305, 745, 1649, 1821
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 5 cut(s) 512, 734, 1867, 2600, 2651
TscAI CASTG 8 cut(s) 100, 160, 691, 1038, 1087, 1424, 2352, 2854
TseFI GTSAC 3 cut(s) 587, 686, 1437
TseI GCWGC 9 cut(s) 9, 170, 209, 212, 215, 237, 1225, 2440, 2909
Tsp45I GTSAC 3 cut(s) 587, 686, 1437
TspGWI ACGGA 3 cut(s) 1455, 1550, 2894
TspRI CASTG 8 cut(s) 100, 160, 691, 1038, 1087, 1424, 2352, 2854
Vha464I CTTAAG 1 cut(s) 425
VpaK11BI GGWCC 3 cut(s) 791, 824, 2112
VspI ATTAAT 2 cut(s) 71, 707
XagI CCTNNNNNAGG 1 cut(s) 2917
XapI RAATTY 8 cut(s) 518, 602, 677, 1261, 1535, 1906, 2029, 2194
XceI RCATGY 4 cut(s) 244, 292, 1289, 2060
XmiI GTMKAC 2 cut(s) 1606, 2752
XmnI GAANNNNTTC 4 cut(s) 1103, 1535, 1539, 2093
XspI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
ZrmI AGTACT 1 cut(s) 1651
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.