AT2G16740

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0017042
Physical Location & Seq
Forward (+)
7267722 .. 7269055
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G16740.1

Sequence Viewer

Length: 447 bp
ATGGCAACGAGACGGATATTGAAAGAATTGAAGGAGTTGCAAAGAGACCCTCCTGTATCATGCAGTGCAGGTCCAACAGGAGAAGATATGTTCCACTGGCAAGCTACTATAATGGGTCCGAATGAAAGTCCTTACTCCGGAGGTGTTTTCCTTGTCAATATTCATTTCCCTCCTGATTATCCTTTTAAACCTCCCAAGGTTGTATTCAGAACCAAAGTGTTTCACCCAAACATCAACAGTAATGGAAACATATGTTTGGACATTCTCAAAGACCAATGGAGCCCTGCCCTTACCATTTCTAAGGTGCTTCTCTCTATATGCTCTCTTCTAACAGATCCAAACCCTGACGACCCTCTGGTGCCAGAGATAGCTCACATATACAAAACTGACAAGACCAAATATGAAGCCATGGCTCGAAGCTGGACCCAGAAGTATGCCTTGTTTTAA

Protein Analysis

148

Amino Acids

16.76

Weight (kDa)

7.72

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 4.1e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017042)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16740
fragaria_vesca FvH4_2g01240
malus_domestica MD05G1121200.v1.1
prunus_persica Prupe.8G165100_v2.0.a1
pyrus_communis pycom05g11540 pycom10g10670
rosa_chinensis RchiOBHm_Chr6g0251901
rosa_laevigata RLG00000015421
rosa_multiflora Rmu_ssc0000303.1_g000012
rosa_roxburghii Rroxscaffold_7G00216320
rosa_rugosa Rorug05G0504900
rosa_samantha Rh6BG014100
rosa_wichuraiana Rw6G001540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 358
AccIII TCCGGA 1 cut(s) 137
AclWI GGATC 1 cut(s) 329
AfiI CCNNNNNNNGG 1 cut(s) 137
AgsI TTSAA 2 cut(s) 22, 31
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AluBI AGCT 3 cut(s) 104, 371, 420
AluI AGCT 3 cut(s) 104, 371, 420
Alw26I GTCTC 2 cut(s) 4, 39
AlwI GGATC 1 cut(s) 329
Aor13HI TCCGGA 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 71, 116, 423
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 3 cut(s) 71, 116, 423
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 284
BcoDI GTCTC 2 cut(s) 4, 39
BfuAI ACCTGC 1 cut(s) 59
Bme18I GGWCC 3 cut(s) 71, 116, 423
BmgT120I GGNCC 3 cut(s) 71, 116, 423
BmiI GGNNCC 4 cut(s) 117, 281, 360, 425
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 195, 408
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 101
BseAI TCCGGA 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 195, 408
BseLI CCNNNNNNNGG 1 cut(s) 137
BseNI ACTGG 1 cut(s) 101
BsgI GTGCAG 1 cut(s) 87
BshNI GGYRCC 1 cut(s) 358
BsiSI CCGG 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 2 cut(s) 4, 39
BsmBI CGTCTC 1 cut(s) 4
Bso31I GGTCTC 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 284
Bsp13I TCCGGA 1 cut(s) 137
Bsp143I GATC 1 cut(s) 334
Bsp19I CCATGG 1 cut(s) 408
BspEI TCCGGA 1 cut(s) 137
BspLI GGNNCC 4 cut(s) 117, 281, 360, 425
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 195, 408
BssMI GATC 1 cut(s) 334
BssT1I CCWWGG 2 cut(s) 195, 408
Bst4CI ACNGT 1 cut(s) 239
Bst6I CTCTTC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 1 cut(s) 300
BstDSI CCRYGG 1 cut(s) 408
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 2 cut(s) 4, 39
BstMBI GATC 1 cut(s) 334
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BtgI CCRYGG 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 94
BveI ACCTGC 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 102
Cfr13I GGNCC 3 cut(s) 71, 116, 423
CviAII CATG 2 cut(s) 60, 409
CviJI RGCY 6 cut(s) 104, 282, 371, 407, 413, 420
CviKI_1 RGCY 6 cut(s) 104, 282, 371, 407, 413, 420
DdeI CTNAG 1 cut(s) 300
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
DraI TTTAAA 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 2 cut(s) 195, 408
Eco24I GRGCYC 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 71, 116, 423
EcoT14I CCWWGG 2 cut(s) 195, 408
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 195, 408
Esp3I CGTCTC 1 cut(s) 4
FaeI CATG 2 cut(s) 63, 412
FalI AAGNNNNNCTT 1 cut(s) 422
FatI CATG 2 cut(s) 59, 408
FauNDI CATATG 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 284
HapII CCGG 1 cut(s) 138
Hin1II CATG 2 cut(s) 63, 412
HpaII CCGG 1 cut(s) 138
HphI GGTGA 1 cut(s) 215
Hpy188I TCNGA 2 cut(s) 120, 209
Hpy188III TCNNGA 2 cut(s) 138, 173
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4V TGCA 3 cut(s) 40, 63, 68
HpyF3I CTNAG 1 cut(s) 300
Hsp92II CATG 2 cut(s) 63, 412
Kpn2I TCCGGA 1 cut(s) 137
Kzo9I GATC 1 cut(s) 334
LmnI GCTCC 1 cut(s) 279
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 2 cut(s) 95, 317
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 284
MluCI AATT 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 5 cut(s) 60, 134, 180, 201, 363
MroI TCCGGA 1 cut(s) 137
MseI TTAA 2 cut(s) 186, 445
MspI CCGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 408
NdeI CATATG 1 cut(s) 251
NdeII GATC 1 cut(s) 334
NlaIII CATG 2 cut(s) 63, 412
NlaIV GGNNCC 4 cut(s) 117, 281, 360, 425
PspN4I GGNNCC 4 cut(s) 117, 281, 360, 425
PspPI GGNCC 3 cut(s) 71, 116, 423
PsuI RGATCY 1 cut(s) 334
SaqAI TTAA 2 cut(s) 186, 445
Sau3AI GATC 1 cut(s) 334
Sau96I GGNCC 3 cut(s) 71, 116, 423
SduI GDGCHC 1 cut(s) 284
SetI ASST 8 cut(s) 73, 106, 145, 193, 201, 306, 373, 422
SinI GGWCC 3 cut(s) 71, 116, 423
Sse9I AATT 1 cut(s) 26
SspI AATATT 1 cut(s) 160
StyI CCWWGG 2 cut(s) 195, 408
TaaI ACNGT 1 cut(s) 239
TaqI TCGA 1 cut(s) 415
TasI AATT 1 cut(s) 26
Tru1I TTAA 2 cut(s) 186, 445
Tru9I TTAA 2 cut(s) 186, 445
TscAI CASTG 2 cut(s) 70, 101
TspDTI ATGAA 3 cut(s) 138, 152, 417
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 2 cut(s) 70, 101
VpaK11BI GGWCC 3 cut(s) 71, 116, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.