AT2G19400

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011798
Physical Location & Seq
Reverse (-)
8399100 .. 8402664
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G19400.1

Sequence Viewer

Length: 1584 bp
ATGGAGAATCAAGAGGAAGATGAGGTGGTGCTGGCGAAGGTGACGTCGGAGGTTGAGGATAATTTTGAGGATGAAGGGTTGGTTTCAAATTCAACATTGGAGAAAGTTGCTGCAGCGAAGAAGTATATTGAAAACCATTACAATAGACGCATGAGACACATTCAACAACGTAAAGAAAGGCGTTGGGTGCTAGAACAAAAGATTGCATCTTTAGATGTATCAGAAAAAGAACAACTCGAGTTGCTGGAGGATTTGCAGAGGAAAGAGACAGAGTATACTAGATTAATGAGGAACAGGCTTTGCGTTGACGATTTTGATCTTCTGAGTATTATCGGGAGAGGAGCTTTTGGTGAGGTAAGACTCTGCCGTGAGAAAAAGACAGGCAACATTTATGCTATGAAGAAGCTTAAGAAGTCTGAAATGCTTAGTAGGGGGCAAGTTGAACATGTTAGAGCTGAGAGGAACTTGCTAGCCGAAGTTGCTAGTGACTGTATTGTGAAGCTTTATTATTCGTTCCAAGATCCAGAATATTTGTACCTTATCATGGAGTATCTGTCTGGTGGTGATGTGATGACTTTGCTTATGAGGGAAGAGACTTTGACGGAAACTGTGGCTCGGTTTTACATTGCCCAAAGTGTTTTGGCTATTGAGTCCATACATAAGCATAATTATGTTCACAGGGATATAAAACCAGACAATCTTCTGTTGGACAAGTATGGTCATATGAAGCTGTCGGATTTTGGTCTTTGTAAGCCTCTCGATTGTAGAAATATTTCGGCTATGAACGTAAATGAACCTTTGAATGATGAGAATATAAATGAATCAATCGATGGTGATGAGAACTGCTCGATAGGACGTCGTGGAAGGCGTTGGAAGAGCCCACTGGAACAGCTGCAGCATTGGCAGATAAACAGAAGAAAACTGGCATATTCTACGGTAGGTACCCCAGATTATATTGCACCAGAGGTGTTGCTGAAGAAGGGATACGGTGTAGAGTGTGACTGGTGGTCTTTGGGGGCCATTATGTATGAGATGCTCGTTGGTTATCCACCATTTTACTCCGATGACCCTGTAACAACATGTAGAAAGATTGTGAGCTGGAGAACTCATTTAGTATTCCCCGAGGGTGCAAGGTTGACACCTGAGGCAAGAGATCTCATTTGTAGGTTGCTTTGTGATTCTGAGCACAGGCTTGGTTCTCATGGAGCCGGAGCCGAGCAAATCAAAGCTCATACTTGGTTCAAAGATGTGGAATGGGAAAAACTCTATGAGATGGATGCAGCTTTTAAGCCAGTGGTCAATGGAGAACTTGATACCCAGAATTTTATGAAATTCGATGAGGTGGAGTGTCCAAAACCAGCAAGAACAGGATCTGGACCGTCATGGAAGGTGAGCATAACCCCTCAAAATATCAATTTTGTGGGCTATACGTACAGAAACTTTGACGCTGTTCGTGGTTCTCGCCATTCTTTAGATATCAAAGGAAGCGTTTCACCTCCACGCTCATCAACTGACTCTACCCGAAGTGACTCTGCAATTGATTACACAAAACTCTCAACTGGTGGCGATGGCTCACAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

527

Amino Acids

60.66

Weight (kDa)

5.76

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 107 - 254 2.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 108 - 254 1e-21 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 179 - 257 1.4e-06 ABC1 atypical kinase-like domain
Pkinase PF00069 303 - 414 2.8e-19 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 47, 859
Acc65I GGTACC 1 cut(s) 941
AccB1I GGYRCC 1 cut(s) 941
AccI GTMKAC 1 cut(s) 275
AclWI GGATC 2 cut(s) 515, 1378
AcsI RAATTY 3 cut(s) 88, 1321, 1331
AcuI CTGAAG 1 cut(s) 995
AcyI GRCGYC 2 cut(s) 44, 856
AfaI GTAC 3 cut(s) 536, 943, 1433
AfiI CCNNNNNNNGG 1 cut(s) 544
AflII CTTAAG 1 cut(s) 407
AflIII ACRYGT 2 cut(s) 445, 1079
AgsI TTSAA 7 cut(s) 87, 93, 131, 164, 443, 802, 1243
AhdI GACNNNNNGTC 1 cut(s) 1006
AluBI AGCT 9 cut(s) 344, 406, 455, 502, 730, 892, 1098, 1229, 1283
AluI AGCT 9 cut(s) 344, 406, 455, 502, 730, 892, 1098, 1229, 1283
Alw21I GWGCWC 1 cut(s) 1188
Alw26I GTCTC 3 cut(s) 148, 260, 587
AlwI GGATC 2 cut(s) 515, 1378
AlwNI CAGNNNCTG 1 cut(s) 1373
Ama87I CYCGRG 2 cut(s) 236, 1121
AoxI GGCC 1 cut(s) 1017
ApeKI GCWGC 5 cut(s) 110, 113, 892, 895, 1280
ApoI RAATTY 3 cut(s) 88, 1321, 1331
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
AseI ATTAAT 1 cut(s) 284
Asp700I GAANNNNTTC 2 cut(s) 772, 1489
Asp718I GGTACC 1 cut(s) 941
AspS9I GGNCC 2 cut(s) 1017, 1376
AsuHPI GGTGA 6 cut(s) 52, 362, 575, 845, 1402, 1485
AsuNHI GCTAGC 1 cut(s) 469
AvaI CYCGRG 2 cut(s) 236, 1121
AvaII GGWCC 1 cut(s) 1376
AxyI CCTNAGG 1 cut(s) 1143
BanI GGYRCC 1 cut(s) 941
BanII GRGCYC 1 cut(s) 881
BarI GAAGNNNNNNTAC 2 cut(s) 968, 1000
Bbv12I GWGCWC 1 cut(s) 1188
BbvI GCAGC 5 cut(s) 97, 125, 879, 907, 1292
BccI CCATC 3 cut(s) 824, 1267, 1562
BceAI ACGGC 1 cut(s) 351
BciVI GTATCC 1 cut(s) 977
BcoDI GTCTC 3 cut(s) 148, 260, 587
BfaI CTAG 4 cut(s) 191, 279, 470, 483
BfmI CTRYAG 2 cut(s) 111, 893
BfrI CTTAAG 1 cut(s) 407
BfuI GTATCC 1 cut(s) 977
BglII AGATCT 1 cut(s) 1153
BisI GCNGC 5 cut(s) 111, 114, 893, 896, 1281
BlsI GCNGC 5 cut(s) 112, 115, 894, 897, 1282
Bme18I GGWCC 1 cut(s) 1376
BmeRI GACNNNNNGTC 1 cut(s) 1006
BmeT110I CYCGRG 2 cut(s) 236, 1121
BmgT120I GGNCC 2 cut(s) 1017, 1376
BmiI GGNNCC 4 cut(s) 943, 1018, 1207, 1213
BmsI GCATC 3 cut(s) 215, 1023, 1267
BmtI GCTAGC 1 cut(s) 473
BplI GAGNNNNNCTC 2 cut(s) 830, 862
BpmI CTGGAG 2 cut(s) 266, 1120
Bsa29I ATCGAT 1 cut(s) 828
BsaAI YACGTR 1 cut(s) 1431
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 2 cut(s) 44, 856
BsaJI CCNNGG 1 cut(s) 1122
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse1I ACTGG 5 cut(s) 888, 927, 1007, 1292, 1564
Bse21I CCTNAGG 1 cut(s) 1143
Bse3DI GCAATG 1 cut(s) 624
Bse8I GATNNNNATC 1 cut(s) 315
BseCI ATCGAT 1 cut(s) 828
BseDI CCNNGG 1 cut(s) 1122
BseGI GGATG 2 cut(s) 76, 1282
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 544
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 4 cut(s) 314, 447, 1134, 1173
BseNI ACTGG 5 cut(s) 888, 927, 1007, 1292, 1564
BseRI GAGGAG 1 cut(s) 354
BseXI GCAGC 5 cut(s) 97, 125, 879, 907, 1292
BshFI GGCC 1 cut(s) 1019
BshNI GGYRCC 1 cut(s) 941
BshVI ATCGAT 1 cut(s) 828
BsiHKAI GWGCWC 1 cut(s) 1188
BsiHKCI CYCGRG 2 cut(s) 236, 1121
BsiSI CCGG 1 cut(s) 1209
BslI CCNNNNNNNGG 1 cut(s) 544
BsmAI GTCTC 3 cut(s) 148, 260, 587
BsnI GGCC 1 cut(s) 1019
BsoBI CYCGRG 2 cut(s) 236, 1121
Bsp1286I GDGCHC 2 cut(s) 881, 1188
Bsp143I GATC 4 cut(s) 316, 520, 1153, 1370
BspANI GGCC 1 cut(s) 1019
BspCNI CTCAG 4 cut(s) 315, 448, 1135, 1174
BspDI ATCGAT 1 cut(s) 828
BspLI GGNNCC 4 cut(s) 943, 1018, 1207, 1213
BspMAI CTGCAG 2 cut(s) 115, 897
BspOI GCTAGC 1 cut(s) 473
BspPI GGATC 2 cut(s) 515, 1378
BspQI GCTCTTC 1 cut(s) 869
BspT107I GGYRCC 1 cut(s) 941
BspTI CTTAAG 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 624
BsrI ACTGG 5 cut(s) 888, 927, 1007, 1292, 1564
BssECI CCNNGG 1 cut(s) 1122
BssMI GATC 4 cut(s) 316, 520, 1153, 1370
BssNAI GTATAC 1 cut(s) 276
BssNI GRCGYC 2 cut(s) 44, 856
Bst1107I GTATAC 1 cut(s) 276
Bst4CI ACNGT 5 cut(s) 491, 610, 937, 989, 1380
Bst6I CTCTTC 2 cut(s) 585, 869
BstACI GRCGYC 2 cut(s) 44, 856
BstAFI CTTAAG 1 cut(s) 407
BstBAI YACGTR 1 cut(s) 1431
BstC8I GCNNGC 2 cut(s) 33, 471
BstDEI CTNAG 5 cut(s) 323, 425, 456, 1143, 1182
BstF5I GGATG 2 cut(s) 76, 1282
BstKTI GATC 4 cut(s) 319, 523, 1156, 1373
BstMAI GTCTC 3 cut(s) 148, 260, 587
BstMBI GATC 4 cut(s) 316, 520, 1153, 1370
BstMWI GCNNNNNNNGC 3 cut(s) 187, 479, 901
BstNSI RCATGY 2 cut(s) 449, 1083
BstSFI CTRYAG 2 cut(s) 111, 893
BstSNI TACGTA 1 cut(s) 1431
BstV1I GCAGC 5 cut(s) 97, 125, 879, 907, 1292
BstX2I RGATCY 3 cut(s) 520, 1153, 1370
BstYI RGATCY 3 cut(s) 520, 1153, 1370
BstZ17I GTATAC 1 cut(s) 276
Bsu15I ATCGAT 1 cut(s) 828
Bsu36I CCTNAGG 1 cut(s) 1143
BsuI GTATCC 1 cut(s) 977
BsuRI GGCC 1 cut(s) 1019
BsuTUI ATCGAT 1 cut(s) 828
BtsCI GGATG 2 cut(s) 76, 1282
BtsIMutI CAGTG 2 cut(s) 881, 1299
Cac8I GCNNGC 2 cut(s) 33, 471
CaiI CAGNNNCTG 1 cut(s) 1373
Cfr13I GGNCC 2 cut(s) 1017, 1376
ClaI ATCGAT 1 cut(s) 828
CseI GACGC 2 cut(s) 156, 1454
Csp6I GTAC 3 cut(s) 535, 942, 1432
CviAII CATG 6 cut(s) 151, 446, 544, 1080, 1202, 1383
CviQI GTAC 3 cut(s) 535, 942, 1432
DdeI CTNAG 5 cut(s) 323, 425, 456, 1143, 1182
DpnI GATC 4 cut(s) 318, 522, 1155, 1372
DpnII GATC 4 cut(s) 316, 520, 1153, 1370
DriI GACNNNNNGTC 1 cut(s) 1006
Eam1104I CTCTTC 2 cut(s) 585, 869
Eam1105I GACNNNNNGTC 1 cut(s) 1006
EarI CTCTTC 2 cut(s) 585, 869
Eco105I TACGTA 1 cut(s) 1431
Eco24I GRGCYC 1 cut(s) 881
Eco32I GATATC 1 cut(s) 1477
Eco47I GGWCC 1 cut(s) 1376
Eco57I CTGAAG 1 cut(s) 995
Eco81I CCTNAGG 1 cut(s) 1143
Eco88I CYCGRG 2 cut(s) 236, 1121
EcoRV GATATC 1 cut(s) 1477
EcoT38I GRGCYC 1 cut(s) 881
FaeI CATG 6 cut(s) 154, 449, 547, 1083, 1205, 1386
FatI CATG 6 cut(s) 150, 445, 543, 1079, 1201, 1382
FauNDI CATATG 1 cut(s) 723
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 5 cut(s) 111, 114, 893, 896, 1281
FokI GGATG 2 cut(s) 83, 1289
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 5 cut(s) 111, 114, 893, 896, 1281
FspBI CTAG 4 cut(s) 191, 279, 470, 483
GluI GCNGC 5 cut(s) 111, 114, 893, 896, 1281
GsuI CTGGAG 2 cut(s) 266, 1120
HaeIII GGCC 1 cut(s) 1019
HapII CCGG 1 cut(s) 1209
HgaI GACGC 2 cut(s) 156, 1454
Hin1I GRCGYC 2 cut(s) 44, 856
Hin1II CATG 6 cut(s) 154, 449, 547, 1083, 1205, 1386
HincII GTYRAC 2 cut(s) 307, 1137
HindII GTYRAC 2 cut(s) 307, 1137
HindIII AAGCTT 2 cut(s) 404, 500
HinfI GANTC 7 cut(s) 7, 360, 650, 821, 1178, 1514, 1529
HpaII CCGG 1 cut(s) 1209
HphI GGTGA 6 cut(s) 52, 362, 575, 845, 1402, 1485
Hpy166II GTNNAC 4 cut(s) 276, 307, 676, 1137
Hpy188I TCNGA 7 cut(s) 49, 223, 324, 418, 736, 1063, 1183
Hpy188III TCNNGA 5 cut(s) 11, 334, 524, 758, 1374
Hpy8I GTNNAC 4 cut(s) 276, 307, 676, 1137
Hpy99I CGWCG 2 cut(s) 49, 861
HpyAV CCTTC 5 cut(s) 31, 68, 858, 973, 1381
HpyCH4III ACNGT 5 cut(s) 491, 610, 937, 989, 1380
HpyCH4IV ACGT 5 cut(s) 44, 169, 786, 856, 1430
HpyCH4V TGCA 8 cut(s) 113, 206, 256, 895, 959, 1130, 1280, 1535
HpyF10VI GCNNNNNNNGC 3 cut(s) 187, 479, 901
HpyF3I CTNAG 5 cut(s) 323, 425, 456, 1143, 1182
HpySE526I ACGT 5 cut(s) 44, 169, 786, 856, 1430
Hsp92I GRCGYC 2 cut(s) 44, 856
Hsp92II CATG 6 cut(s) 154, 449, 547, 1083, 1205, 1386
KpnI GGTACC 1 cut(s) 945
Kzo9I GATC 4 cut(s) 316, 520, 1153, 1370
LguI GCTCTTC 1 cut(s) 869
LmnI GCTCC 3 cut(s) 341, 1205, 1211
Lsp1109I GCAGC 5 cut(s) 97, 125, 879, 907, 1292
LweI GCATC 3 cut(s) 215, 1023, 1267
MaeI CTAG 4 cut(s) 191, 279, 470, 483
MaeII ACGT 5 cut(s) 44, 169, 786, 856, 1430
MaeIII GTNAC 5 cut(s) 40, 485, 998, 1072, 1526
MalI GATC 4 cut(s) 318, 522, 1155, 1372
MboI GATC 4 cut(s) 316, 520, 1153, 1370
MboII GAAGA 9 cut(s) 29, 130, 311, 412, 602, 692, 886, 927, 988
MfeI CAATTG 1 cut(s) 1536
MflI RGATCY 3 cut(s) 520, 1153, 1370
MhlI GDGCHC 2 cut(s) 881, 1188
MluCI AATT 7 cut(s) 61, 88, 667, 1321, 1331, 1414, 1536
MlyI GAGTC 4 cut(s) 354, 659, 1508, 1523
MmeI TCCRAC 4 cut(s) 27, 687, 714, 851
MroXI GAANNNNTTC 2 cut(s) 772, 1489
MseI TTAA 3 cut(s) 284, 408, 1287
MslI CAYNNNNRTG 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 892
MspCI CTTAAG 1 cut(s) 407
MspI CCGG 1 cut(s) 1209
MunI CAATTG 1 cut(s) 1536
MwoI GCNNNNNNNGC 3 cut(s) 187, 479, 901
NdeI CATATG 1 cut(s) 723
NdeII GATC 4 cut(s) 316, 520, 1153, 1370
NheI GCTAGC 1 cut(s) 469
NlaIII CATG 6 cut(s) 154, 449, 547, 1083, 1205, 1386
NlaIV GGNNCC 4 cut(s) 943, 1018, 1207, 1213
NmeAIII GCCGAG 1 cut(s) 1240
NmuCI GTSAC 4 cut(s) 40, 485, 998, 1526
NspI RCATGY 2 cut(s) 449, 1083
PaeR7I CTCGAG 1 cut(s) 236
PciI ACATGT 2 cut(s) 445, 1079
PciSI GCTCTTC 1 cut(s) 869
PcsI WCGNNNNNNNCGW 1 cut(s) 865
PdmI GAANNNNTTC 2 cut(s) 772, 1489
PfeI GAWTC 3 cut(s) 7, 821, 1178
PkrI GCNGC 5 cut(s) 112, 115, 894, 897, 1282
PleI GAGTC 4 cut(s) 354, 658, 1508, 1523
PpsI GAGTC 4 cut(s) 354, 658, 1508, 1523
Ppu21I YACGTR 1 cut(s) 1431
PscI ACATGT 2 cut(s) 445, 1079
PshBI ATTAAT 1 cut(s) 284
PspN4I GGNNCC 4 cut(s) 943, 1018, 1207, 1213
PspPI GGNCC 2 cut(s) 1017, 1376
PspXI VCTCGAGB 1 cut(s) 236
PstI CTGCAG 2 cut(s) 115, 897
PstNI CAGNNNCTG 1 cut(s) 1373
PsuI RGATCY 3 cut(s) 520, 1153, 1370
PvuII CAGCTG 1 cut(s) 892
RsaI GTAC 3 cut(s) 536, 943, 1433
RsaNI GTAC 3 cut(s) 535, 942, 1432
RseI CAYNNNNRTG 1 cut(s) 669
SapI GCTCTTC 1 cut(s) 869
SaqAI TTAA 3 cut(s) 284, 408, 1287
SatI GCNGC 5 cut(s) 111, 114, 893, 896, 1281
Sau3AI GATC 4 cut(s) 316, 520, 1153, 1370
Sau96I GGNCC 2 cut(s) 1017, 1376
SchI GAGTC 4 cut(s) 354, 659, 1508, 1523
SduI GDGCHC 2 cut(s) 881, 1188
SfaNI GCATC 3 cut(s) 215, 1023, 1267
SfcI CTRYAG 2 cut(s) 111, 893
Sfr274I CTCGAG 1 cut(s) 236
SinI GGWCC 1 cut(s) 1376
SlaI CTCGAG 1 cut(s) 236
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 2 cut(s) 236, 407
SmoI CTYRAG 2 cut(s) 236, 407
SnaBI TACGTA 1 cut(s) 1431
Sse9I AATT 7 cut(s) 61, 88, 667, 1321, 1331, 1414, 1536
SspI AATATT 2 cut(s) 530, 772
SspMI CTAG 4 cut(s) 191, 279, 470, 483
TaaI ACNGT 5 cut(s) 491, 610, 937, 989, 1380
TaiI ACGT 5 cut(s) 47, 172, 789, 859, 1433
TaqI TCGA 5 cut(s) 237, 759, 828, 848, 1335
TasI AATT 7 cut(s) 61, 88, 667, 1321, 1331, 1414, 1536
TfiI GAWTC 3 cut(s) 7, 821, 1178
Tru1I TTAA 3 cut(s) 284, 408, 1287
Tru9I TTAA 3 cut(s) 284, 408, 1287
TscAI CASTG 2 cut(s) 888, 1299
TseFI GTSAC 4 cut(s) 40, 485, 998, 1526
TseI GCWGC 5 cut(s) 110, 113, 892, 895, 1280
Tsp45I GTSAC 4 cut(s) 40, 485, 998, 1526
TspDTI ATGAA 7 cut(s) 87, 413, 740, 797, 807, 834, 1343
TspGWI ACGGA 1 cut(s) 617
TspRI CASTG 2 cut(s) 888, 1299
Vha464I CTTAAG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 1376
VspI ATTAAT 1 cut(s) 284
XapI RAATTY 3 cut(s) 88, 1321, 1331
XceI RCATGY 2 cut(s) 449, 1083
XhoI CTCGAG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 275
XmnI GAANNNNTTC 2 cut(s) 772, 1489
XspI CTAG 4 cut(s) 191, 279, 470, 483
ZraI GACGTC 2 cut(s) 45, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.