Rh2CG090400

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011798
Physical Location & Seq
Forward (+)
7432981 .. 7440058
7078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG090400.1

Sequence Viewer

Length: 1608 bp
ATGGAGAGGAGGCAGAACAAGTCGGAGGCGGAGGAGGGAATGGAGGAGGAGGAGGAGCAGGTGGGTCCCACGGGCGGGGCCGGGGCTGAGGACGGTCAGGTCGGGTCGAGCTTGACGCTGGAGAGGGTGGCTGCGGCCAAGCAGTTTATAGAGAGCCACTACAAGGCTCAGATGAAGCACATCCAGGATCGTAAGCAGAGACGCTCAGTGCTGGAAAAGAAGCTGGCCTCTGAACATGTACCACAGGAGGAACAGGTCAATCTGTTGAAGGATTTGGAACGCAAAGAGACTGAATATATGCGACTTAAAAGGCACAAGATTTGTGTTGAGGATTTCGACCTTCTCACCATAATTGGCAGAGGAGCCTTTGGAGAGGTTAGATTGTGTCGCGAGAAGATATCAGGCACCATTTTTGCCATGAAAAAGTTGAAGAAGTCAGAAATGCTCAGCAGGGGACAGGTTGAACATGTTAGAGCTGAAAGGAATTTGCTTGCAGAAGTTGCCAGTCACTGCATTGTGAAACTTTACTATTCATTTCAAGACGCTGAATACTTGTATCTAATTATGGAGTATCTACCTGGTGGAGACATGATGACTTTGCTTATAAGAGAAGAGACTTTGACTGAAACTGTTGCTAGATTTTATATTGCTCAAAGTGTTCTGGCAATAGAATCTATTCATAAACATAACTATATTCACAGAGATATAAAACCAGACAATCTTTTATTAGACCAAAATGGTCACATGAAGCTCTCTGATTTTGGTCTTTGCAAGCCTCTTGATTGCTCAAATTTATCTTCCATAAATGAAAATGAAGTGCTCGATGATGATAACTTGCATGAGACAATGGATATTGATGAATCCTCCCCAGAGACCAAAAATGGGAGGCCCTGGAAAAGCCCGCTTGAGCAGCTTCAGCATTGGCAGATAAACAGGAGAACACTGGCATATTCAACAGTTGGCACACCGGATTACATTGCTCCAGAAGTATTATTGAAGAAAGGATATGGCATGGAGTGTGATTGGTGGTCACTGGGTGCGATAATGTATGAGATGCTTGTTGGTTATCCCCCATTTTACTCTGATGATCCAGTAACAACATGCAGAAAGATAGTGCATTGGAAAAATCACTTGAAATTTCCCGTGGAGGCAAGGTTGACGCCTGAAGCAAAGGATCTGATTAGTAGGTTTCTCTGTGATGTTGAACATAGGCTTCGTACAGCAGACCAAATCAAGGCGCATCCTTGGTTCAGAGATGTCCAGTGGGACAGACTTTATGAAATGGAGGCAGCATTTAAACCAGAGGTCAATGGGGAACTCGATACTCAAAATTTTATGAAATATGATGAGGTAGATGCACCAGCGCCCGGAAGAATTGGATCTGGACTAATGAGAAAGATGTTGTTGACTCCTAAAGATCTCAGTTTTGTTGGCTATACATACAAAAATTTTGAGGCTGTCAAAGGTTTAAAGAGGAGTGCATCACTAAATCGGTCCTCCATTGACTCTGCAAACAGTGATTCTGTACTGGACTACTCCAAAAGGTATTCGGTAGATGACATGGAAGCTCAGGTGCTTGCTGATTCAGGGGACGTCATGTCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

535

Amino Acids

61.51

Weight (kDa)

5.48

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 114 - 263 3.1e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 115 - 261 1.4e-22 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 186 - 261 2.7e-06 ABC1 atypical kinase-like domain
Pkinase PF00069 311 - 417 7.7e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AarI CACCTGC 1 cut(s) 49
AasI GACNNNNNNGTC 1 cut(s) 98
AatII GACGTC 1 cut(s) 1596
Acc36I ACCTGC 1 cut(s) 49
AccB1I GGYRCC 1 cut(s) 404
AccII CGCG 1 cut(s) 390
AciI CCGC 4 cut(s) 29, 75, 134, 902
AclWI GGATC 4 cut(s) 195, 1082, 1182, 1387
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 5 cut(s) 484, 790, 1136, 1330, 1447
AcuI CTGAAG 2 cut(s) 899, 1185
AcyI GRCGYC 2 cut(s) 1160, 1593
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 3 cut(s) 240, 1219, 1527
AfiI CCNNNNNNNGG 6 cut(s) 74, 75, 163, 882, 1234, 1367
AflIII ACRYGT 2 cut(s) 235, 466
AgsI TTSAA 8 cut(s) 268, 430, 464, 539, 954, 997, 1135, 1205
AhdI GACNNNNNGTC 1 cut(s) 1597
AjnI CCWGG 3 cut(s) 183, 577, 890
AluBI AGCT 6 cut(s) 111, 223, 476, 751, 913, 1568
AluI AGCT 6 cut(s) 111, 223, 476, 751, 913, 1568
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 6 cut(s) 193, 281, 579, 608, 836, 866
AlwI GGATC 4 cut(s) 195, 1082, 1182, 1387
AlwNI CAGNNNCTG 1 cut(s) 510
AoxI GGCC 4 cut(s) 78, 135, 225, 887
ApeKI GCWGC 3 cut(s) 131, 910, 1289
ApoI RAATTY 5 cut(s) 484, 790, 1136, 1330, 1447
Asp700I GAANNNNTTC 1 cut(s) 675
AspLEI GCGC 2 cut(s) 1240, 1366
AspS9I GGNCC 4 cut(s) 65, 78, 888, 1494
AsuC2I CCSGG 2 cut(s) 82, 1368
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 2 cut(s) 65, 1494
BanI GGYRCC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 822
BbvCI CCTCAGC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 118, 922, 1301
BciT130I CCWGG 3 cut(s) 185, 579, 892
BcnI CCSGG 2 cut(s) 82, 1368
BcoDI GTCTC 6 cut(s) 193, 281, 579, 608, 836, 866
BfaI CTAG 1 cut(s) 636
BfoI RGCGCY 1 cut(s) 1367
BfuAI ACCTGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 1417
BisI GCNGC 4 cut(s) 132, 135, 911, 1290
BlpI GCTNAGC 1 cut(s) 446
BlsI GCNGC 4 cut(s) 133, 136, 912, 1291
Bme1390I CCNGG 5 cut(s) 82, 185, 579, 892, 1368
Bme18I GGWCC 2 cut(s) 65, 1494
BmeRI GACNNNNNGTC 1 cut(s) 1597
BmgT120I GGNCC 4 cut(s) 65, 78, 888, 1494
BmiI GGNNCC 5 cut(s) 66, 67, 79, 364, 406
BmrFI CCNGG 5 cut(s) 82, 185, 579, 892, 1368
BmrI ACTGGG 1 cut(s) 1043
BmsI GCATC 4 cut(s) 1044, 1249, 1345, 1490
BmuI ACTGGG 1 cut(s) 1043
BpmI CTGGAG 2 cut(s) 140, 966
Bpu10I CCTNAGC 2 cut(s) 87, 1569
Bpu1102I GCTNAGC 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 926
BpuMI CCSGG 2 cut(s) 82, 1368
BsaHI GRCGYC 2 cut(s) 1160, 1593
BsaI GGTCTC 1 cut(s) 866
BsaJI CCNNGG 5 cut(s) 69, 81, 890, 1143, 1244
BsaWI WCCGGW 1 cut(s) 967
BsaXI ACNNNNNCTCC 2 cut(s) 1139, 1169
Bsc4I CCNNNNNNNGG 6 cut(s) 74, 75, 163, 882, 1234, 1367
Bse1I ACTGG 6 cut(s) 504, 948, 1038, 1091, 1261, 1533
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 3 cut(s) 185, 579, 892
BseDI CCNNGG 5 cut(s) 69, 81, 890, 1143, 1244
BseGI GGATG 2 cut(s) 180, 1240
BseLI CCNNNNNNNGG 6 cut(s) 74, 75, 163, 882, 1234, 1367
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 6 cut(s) 78, 182, 219, 460, 1435, 1583
BseNI ACTGG 6 cut(s) 504, 948, 1038, 1091, 1261, 1533
BseRI GAGGAG 8 cut(s) 22, 47, 59, 62, 65, 68, 375, 1489
BseXI GCAGC 3 cut(s) 118, 922, 1301
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 4 cut(s) 80, 137, 227, 889
BshNI GGYRCC 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 822
BsiSI CCGG 3 cut(s) 81, 968, 1368
BslFI GGGAC 4 cut(s) 51, 468, 1280, 1604
BslI CCNNNNNNNGG 6 cut(s) 74, 75, 163, 882, 1234, 1367
BsmAI GTCTC 6 cut(s) 193, 281, 579, 608, 836, 866
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 4 cut(s) 51, 468, 1280, 1604
BsnI GGCC 4 cut(s) 80, 137, 227, 889
Bso31I GGTCTC 1 cut(s) 866
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 5 cut(s) 187, 1087, 1174, 1379, 1417
Bsp1720I GCTNAGC 1 cut(s) 446
Bsp68I TCGCGA 1 cut(s) 390
BspACI CCGC 4 cut(s) 29, 75, 134, 902
BspANI GGCC 4 cut(s) 80, 137, 227, 889
BspCNI CTCAG 6 cut(s) 79, 181, 218, 459, 1434, 1582
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 5 cut(s) 66, 67, 79, 364, 406
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 4 cut(s) 195, 1082, 1182, 1387
BspT107I GGYRCC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 866
BsrDI GCAATG 1 cut(s) 975
BsrI ACTGG 6 cut(s) 504, 948, 1038, 1091, 1261, 1533
BssECI CCNNGG 5 cut(s) 69, 81, 890, 1143, 1244
BssMI GATC 5 cut(s) 187, 1087, 1174, 1379, 1417
BssNI GRCGYC 2 cut(s) 1160, 1593
BssT1I CCWWGG 1 cut(s) 1244
Bst2UI CCWGG 3 cut(s) 185, 579, 892
Bst4CI ACNGT 4 cut(s) 95, 631, 958, 1517
Bst6I CTCTTC 1 cut(s) 606
BstACI GRCGYC 2 cut(s) 1160, 1593
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 5 cut(s) 225, 492, 773, 902, 1578
BstDEI CTNAG 6 cut(s) 87, 168, 205, 446, 1421, 1569
BstDSI CCRYGG 2 cut(s) 69, 1143
BstF5I GGATG 2 cut(s) 180, 1240
BstFNI CGCG 1 cut(s) 390
BstH2I RGCGCY 1 cut(s) 1367
BstHHI GCGC 2 cut(s) 1240, 1366
BstKTI GATC 5 cut(s) 190, 1090, 1177, 1382, 1420
BstMAI GTCTC 6 cut(s) 193, 281, 579, 608, 836, 866
BstMBI GATC 5 cut(s) 187, 1087, 1174, 1379, 1417
BstMWI GCNNNNNNNGC 3 cut(s) 500, 910, 916
BstNI CCWGG 3 cut(s) 185, 579, 892
BstNSI RCATGY 3 cut(s) 239, 470, 1104
BstSCI CCNGG 5 cut(s) 80, 183, 577, 890, 1366
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 3 cut(s) 118, 922, 1301
BstX2I RGATCY 3 cut(s) 1174, 1379, 1417
BstYI RGATCY 3 cut(s) 1174, 1379, 1417
BsuRI GGCC 4 cut(s) 80, 137, 227, 889
BtgI CCRYGG 2 cut(s) 69, 1143
BtsCI GGATG 2 cut(s) 180, 1240
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 6 cut(s) 213, 508, 941, 1031, 1268, 1522
BtuMI TCGCGA 1 cut(s) 390
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 5 cut(s) 225, 492, 773, 902, 1578
CaiI CAGNNNCTG 1 cut(s) 510
CfoI GCGC 2 cut(s) 1240, 1366
Cfr13I GGNCC 4 cut(s) 65, 78, 888, 1494
CseI GACGC 4 cut(s) 124, 210, 551, 1168
CsiI ACCWGGT 1 cut(s) 577
Csp6I GTAC 3 cut(s) 239, 1218, 1526
CviQI GTAC 3 cut(s) 239, 1218, 1526
DdeI CTNAG 6 cut(s) 87, 168, 205, 446, 1421, 1569
DpnI GATC 5 cut(s) 189, 1089, 1176, 1381, 1419
DpnII GATC 5 cut(s) 187, 1087, 1174, 1379, 1417
DraI TTTAAA 2 cut(s) 1297, 1470
DraIII CACNNNGTG 1 cut(s) 1037
DrdI GACNNNNNNGTC 1 cut(s) 98
DriI GACNNNNNGTC 1 cut(s) 1597
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 1 cut(s) 135
Eam1104I CTCTTC 1 cut(s) 606
Eam1105I GACNNNNNGTC 1 cut(s) 1597
EarI CTCTTC 1 cut(s) 606
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 1244
Eco31I GGTCTC 1 cut(s) 866
Eco32I GATATC 1 cut(s) 399
Eco47I GGWCC 2 cut(s) 65, 1494
Eco57I CTGAAG 2 cut(s) 899, 1185
EcoO109I RGGNCCY 2 cut(s) 65, 888
EcoRII CCWGG 3 cut(s) 183, 577, 890
EcoRV GATATC 1 cut(s) 399
EcoT14I CCWWGG 1 cut(s) 1244
ErhI CCWWGG 1 cut(s) 1244
Esp3I CGTCTC 1 cut(s) 193
FaqI GGGAC 4 cut(s) 51, 468, 1280, 1604
FauI CCCGC 2 cut(s) 68, 909
Fnu4HI GCNGC 4 cut(s) 132, 135, 911, 1290
FokI GGATG 2 cut(s) 167, 1227
Fsp4HI GCNGC 4 cut(s) 132, 135, 911, 1290
FspBI CTAG 1 cut(s) 636
GlaI GCGC 2 cut(s) 1239, 1365
GluI GCNGC 4 cut(s) 132, 135, 911, 1290
GsuI CTGGAG 2 cut(s) 140, 966
HaeII RGCGCY 1 cut(s) 1367
HaeIII GGCC 4 cut(s) 80, 137, 227, 889
HapII CCGG 3 cut(s) 81, 968, 1368
HgaI GACGC 4 cut(s) 124, 210, 551, 1168
HhaI GCGC 2 cut(s) 1240, 1366
Hin1I GRCGYC 2 cut(s) 1160, 1593
Hin6I GCGC 2 cut(s) 1238, 1364
HinP1I GCGC 2 cut(s) 1238, 1364
HincII GTYRAC 2 cut(s) 1158, 1407
HindII GTYRAC 2 cut(s) 1158, 1407
HinfI GANTC 6 cut(s) 671, 860, 1408, 1505, 1520, 1583
HpaII CCGG 3 cut(s) 81, 968, 1368
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 2 cut(s) 1158, 1407
Hpy188I TCNGA 9 cut(s) 25, 171, 232, 439, 757, 1084, 1179, 1253, 1603
Hpy188III TCNNGA 5 cut(s) 389, 539, 779, 983, 1383
Hpy8I GTNNAC 2 cut(s) 1158, 1407
HpyAV CCTTC 2 cut(s) 262, 350
HpyCH4III ACNGT 4 cut(s) 95, 631, 958, 1517
HpyCH4IV ACGT 1 cut(s) 1593
HpyCH4V TGCA 9 cut(s) 494, 513, 771, 838, 1104, 1117, 1358, 1481, 1511
HpyF10VI GCNNNNNNNGC 3 cut(s) 500, 910, 916
HpyF3I CTNAG 6 cut(s) 87, 168, 205, 446, 1421, 1569
HpySE526I ACGT 1 cut(s) 1593
Hsp92I GRCGYC 2 cut(s) 1160, 1593
HspAI GCGC 2 cut(s) 1238, 1364
KflI GGGWCCC 1 cut(s) 65
Kzo9I GATC 5 cut(s) 187, 1087, 1174, 1379, 1417
LmnI GCTCC 3 cut(s) 55, 362, 985
Lsp1109I GCAGC 3 cut(s) 118, 922, 1301
LweI GCATC 4 cut(s) 1044, 1249, 1345, 1490
MabI ACCWGGT 1 cut(s) 577
MaeI CTAG 1 cut(s) 636
MaeII ACGT 1 cut(s) 1593
MaeIII GTNAC 4 cut(s) 506, 740, 1029, 1093
MalI GATC 5 cut(s) 189, 1089, 1176, 1381, 1419
MboI GATC 5 cut(s) 187, 1087, 1174, 1379, 1417
MboII GAAGA 6 cut(s) 406, 442, 623, 789, 1009, 1383
MflI RGATCY 3 cut(s) 1174, 1379, 1417
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 8 cut(s) 351, 484, 561, 790, 1136, 1330, 1374, 1447
MlyI GAGTC 2 cut(s) 1402, 1499
MroXI GAANNNNTTC 1 cut(s) 675
MseI TTAA 3 cut(s) 306, 1296, 1469
MspI CCGG 3 cut(s) 81, 968, 1368
MspR9I CCNGG 5 cut(s) 82, 185, 579, 892, 1368
MvaI CCWGG 3 cut(s) 185, 579, 892
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 3 cut(s) 500, 910, 916
NciI CCSGG 2 cut(s) 82, 1368
NdeII GATC 5 cut(s) 187, 1087, 1174, 1379, 1417
NlaIV GGNNCC 5 cut(s) 66, 67, 79, 364, 406
NmuCI GTSAC 3 cut(s) 506, 740, 1029
NruI TCGCGA 1 cut(s) 390
NspI RCATGY 3 cut(s) 239, 470, 1104
PaqCI CACCTGC 1 cut(s) 49
PciI ACATGT 2 cut(s) 235, 466
PdmI GAANNNNTTC 1 cut(s) 675
PfeI GAWTC 4 cut(s) 671, 860, 1520, 1583
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 4 cut(s) 133, 136, 912, 1291
PleI GAGTC 2 cut(s) 1402, 1499
PpsI GAGTC 2 cut(s) 1402, 1499
PpuMI RGGWCCY 1 cut(s) 65
PscI ACATGT 2 cut(s) 235, 466
PsiI TTATAA 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 3 cut(s) 183, 577, 890
PspGI CCWGG 3 cut(s) 183, 577, 890
PspN4I GGNNCC 5 cut(s) 66, 67, 79, 364, 406
PspPI GGNCC 4 cut(s) 65, 78, 888, 1494
PspPPI RGGWCCY 1 cut(s) 65
PstNI CAGNNNCTG 1 cut(s) 510
PsuI RGATCY 3 cut(s) 1174, 1379, 1417
RruI TCGCGA 1 cut(s) 390
RsaI GTAC 3 cut(s) 240, 1219, 1527
RsaNI GTAC 3 cut(s) 239, 1218, 1526
SaqAI TTAA 3 cut(s) 306, 1296, 1469
SatI GCNGC 4 cut(s) 132, 135, 911, 1290
Sau3AI GATC 5 cut(s) 187, 1087, 1174, 1379, 1417
Sau96I GGNCC 4 cut(s) 65, 78, 888, 1494
SchI GAGTC 2 cut(s) 1402, 1499
ScrFI CCNGG 5 cut(s) 82, 185, 579, 892, 1368
SduI GDGCHC 1 cut(s) 822
SexAI ACCWGGT 1 cut(s) 577
SfaNI GCATC 4 cut(s) 1044, 1249, 1345, 1490
SinI GGWCC 2 cut(s) 65, 1494
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
Sse9I AATT 8 cut(s) 351, 484, 561, 790, 1136, 1330, 1374, 1447
SsiI CCGC 4 cut(s) 29, 75, 134, 902
SspMI CTAG 1 cut(s) 636
StyD4I CCNGG 5 cut(s) 80, 183, 577, 890, 1366
StyI CCWWGG 1 cut(s) 1244
TaaI ACNGT 4 cut(s) 95, 631, 958, 1517
TaiI ACGT 1 cut(s) 1596
TaqI TCGA 4 cut(s) 107, 336, 822, 1320
TaqII GACCGA 1 cut(s) 1482
TasI AATT 8 cut(s) 351, 484, 561, 790, 1136, 1330, 1374, 1447
TatI WGTACW 1 cut(s) 1525
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 4 cut(s) 671, 860, 1520, 1583
Tru1I TTAA 3 cut(s) 306, 1296, 1469
Tru9I TTAA 3 cut(s) 306, 1296, 1469
TscAI CASTG 6 cut(s) 213, 515, 948, 1038, 1268, 1522
TseFI GTSAC 3 cut(s) 506, 740, 1029
TseI GCWGC 3 cut(s) 131, 910, 1289
Tsp45I GTSAC 3 cut(s) 506, 740, 1029
TspRI CASTG 6 cut(s) 213, 515, 948, 1038, 1268, 1522
VpaK11BI GGWCC 2 cut(s) 65, 1494
XapI RAATTY 5 cut(s) 484, 790, 1136, 1330, 1447
XceI RCATGY 3 cut(s) 239, 470, 1104
XmnI GAANNNNTTC 1 cut(s) 675
XspI CTAG 1 cut(s) 636
ZraI GACGTC 1 cut(s) 1594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.