AT2G23690

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014021
Physical Location & Seq
Forward (+)
10072057 .. 10073126
1070 bp
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UTR
Exon/CDS
Intron
AT2G23690.1

Sequence Viewer

Length: 492 bp
ATGGGTATATGCAGCTCGTACGAGTCGACACAAGTCGCAACGGCGAAGCTGATCTTACACGACGGAAGGATGATGGAGTTCACAAGTCCGGTCAAAGTTGGTTACGTGTTACAAAAGAATCCGATGTGTTTCATATGTAACTCCGATGATATGGACTTTGACAACGTTGTATCCGCCATTAGCGCCGACGAGGAGTTTCAGCTTGGTCAACTGTACTTCGCTCTTCCTTTAAGTTCGCTTCATCATTCTCTTAAAGCCGAGGAAATGGCTGCATTGGCTGTTAAAGCTAGCTCTGCTCTTATGAGAAGTGGTGGTTCATGTGGCCGAGATAAATGTCGGTGTCGTCGGAAATGTGTGTCTCCGGTGATCTTTTCTGCTCGAAGAGTGGCGGCGGTGGGAGCTAATGGAGAGACAAGGAATGGGAAAAGACGCGGTGGTGGTGGTAGCGGGAGGAGGAAGTATGCGGCGAAGTTGAGTAAGATAGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

163

Amino Acids

17.65

Weight (kDa)

9.28

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 162 5e-28 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23690
fragaria_vesca FvH4_1g14400
malus_domestica MD02G1157500.v1.1 MD15G1272100.v1.1
prunus_persica Prupe.7G146200_v2.0.a1
pyrus_communis pycom02g12450
rosa_chinensis RchiOBHm_Chr2g0103121
rosa_laevigata RLG00000017210
rosa_multiflora Rmu_sc0000388.1_g000007
rosa_roxburghii Rroxscaffold_2G00139910 Rroxscaffold_2G00140190
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160700 Rh2BG167400 Rh2CG167700 Rh2DG166300
rosa_wichuraiana Rw2G012580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 26
AccII CGCG 1 cut(s) 432
AciI CCGC 6 cut(s) 174, 389, 392, 432, 447, 464
AclI AACGTT 1 cut(s) 165
AcoI YGGCCR 1 cut(s) 322
AfaI GTAC 2 cut(s) 20, 215
AflIII ACRYGT 1 cut(s) 105
AluBI AGCT 6 cut(s) 15, 49, 202, 287, 291, 401
AluI AGCT 6 cut(s) 15, 49, 202, 287, 291, 401
Alw26I GTCTC 2 cut(s) 363, 404
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 2 cut(s) 12, 269
AspLEI GCGC 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 376
AsuNHI GCTAGC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 24, 256
BccI CCATC 1 cut(s) 67
BceAI ACGGC 1 cut(s) 57
BciVI GTATCC 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 363, 404
BfaI CTAG 1 cut(s) 288
BfoI RGCGCY 1 cut(s) 186
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 4 cut(s) 13, 270, 390, 465
BlsI GCNGC 4 cut(s) 14, 271, 391, 466
BmtI GCTAGC 1 cut(s) 291
BoxI GACNNNNGTC 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 258
BsaWI WCCGGW 2 cut(s) 88, 361
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 75
BseRI GAGGAG 2 cut(s) 206, 466
BseXI GCAGC 2 cut(s) 24, 256
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 1 cut(s) 324
BsiSI CCGG 2 cut(s) 89, 362
BsiWI CGTACG 1 cut(s) 18
BsmAI GTCTC 2 cut(s) 363, 404
BsnI GGCC 1 cut(s) 324
Bsp143I GATC 2 cut(s) 51, 366
BspACI CCGC 6 cut(s) 174, 389, 392, 432, 447, 464
BspANI GGCC 1 cut(s) 324
BspFNI CGCG 1 cut(s) 432
BspOI GCTAGC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 228
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 2 cut(s) 51, 366
Bst4CI ACNGT 1 cut(s) 213
Bst6I CTCTTC 2 cut(s) 228, 376
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 289
BstF5I GGATG 1 cut(s) 75
BstFNI CGCG 1 cut(s) 432
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 2 cut(s) 54, 369
BstMAI GTCTC 2 cut(s) 363, 404
BstMBI GATC 2 cut(s) 51, 366
BstMWI GCNNNNNNNGC 5 cut(s) 182, 275, 284, 293, 398
BstPAI GACNNNNGTC 1 cut(s) 32
BstUI CGCG 1 cut(s) 432
BstV1I GCAGC 2 cut(s) 24, 256
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 1 cut(s) 324
BtsCI GGATG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 289
CfoI GCGC 1 cut(s) 185
CseI GACGC 1 cut(s) 438
Csp6I GTAC 2 cut(s) 19, 214
CviAII CATG 1 cut(s) 318
CviQI GTAC 2 cut(s) 19, 214
DpnI GATC 2 cut(s) 53, 368
DpnII GATC 2 cut(s) 51, 366
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 228, 376
EarI CTCTTC 2 cut(s) 228, 376
EciI GGCGGA 1 cut(s) 163
FaeI CATG 1 cut(s) 321
FaiI YATR 8 cut(s) 8, 10, 134, 136, 152, 302, 319, 462
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FatI CATG 1 cut(s) 317
FauI CCCGC 1 cut(s) 440
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 4 cut(s) 13, 270, 390, 465
FokI GGATG 1 cut(s) 82
Fsp4HI GCNGC 4 cut(s) 13, 270, 390, 465
FspBI CTAG 1 cut(s) 288
GlaI GCGC 1 cut(s) 184
GluI GCNGC 4 cut(s) 13, 270, 390, 465
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 1 cut(s) 324
HapII CCGG 2 cut(s) 89, 362
HgaI GACGC 1 cut(s) 438
HhaI GCGC 1 cut(s) 185
Hin1II CATG 1 cut(s) 321
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 2 cut(s) 27, 209
HindII GTYRAC 2 cut(s) 27, 209
HinfI GANTC 2 cut(s) 23, 118
HpaII CCGG 2 cut(s) 89, 362
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 3 cut(s) 27, 81, 209
Hpy188I TCNGA 3 cut(s) 123, 145, 348
Hpy8I GTNNAC 3 cut(s) 27, 81, 209
Hpy99I CGWCG 3 cut(s) 65, 191, 348
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 2 cut(s) 105, 165
HpyCH4V TGCA 2 cut(s) 12, 272
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 275, 284, 293, 398
HpySE526I ACGT 2 cut(s) 105, 165
Hsp92II CATG 1 cut(s) 321
HspAI GCGC 1 cut(s) 183
Kzo9I GATC 2 cut(s) 51, 366
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 1 cut(s) 398
LpnPI CCDG 2 cut(s) 102, 375
Lsp1109I GCAGC 2 cut(s) 24, 256
MaeI CTAG 1 cut(s) 288
MaeII ACGT 2 cut(s) 105, 165
MaeIII GTNAC 3 cut(s) 101, 108, 137
MalI GATC 2 cut(s) 53, 368
MboI GATC 2 cut(s) 51, 366
MboII GAAGA 2 cut(s) 215, 393
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 4 cut(s) 184, 253, 444, 447
MseI TTAA 3 cut(s) 230, 252, 282
MspI CCGG 2 cut(s) 89, 362
MvnI CGCG 1 cut(s) 432
MwoI GCNNNNNNNGC 5 cut(s) 182, 275, 284, 293, 398
NdeI CATATG 1 cut(s) 134
NdeII GATC 2 cut(s) 51, 366
NheI GCTAGC 1 cut(s) 287
NlaIII CATG 1 cut(s) 321
NmeAIII GCCGAG 2 cut(s) 283, 350
PciSI GCTCTTC 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PfeI GAWTC 1 cut(s) 118
Pfl23II CGTACG 1 cut(s) 18
PkrI GCNGC 4 cut(s) 14, 271, 391, 466
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 106
PshAI GACNNNNGTC 1 cut(s) 32
Psp1406I AACGTT 1 cut(s) 165
PspLI CGTACG 1 cut(s) 18
RsaI GTAC 2 cut(s) 20, 215
RsaNI GTAC 2 cut(s) 19, 214
SalI GTCGAC 1 cut(s) 25
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 3 cut(s) 230, 252, 282
SatI GCNGC 4 cut(s) 13, 270, 390, 465
Sau3AI GATC 2 cut(s) 51, 366
SchI GAGTC 1 cut(s) 32
SetI ASST 8 cut(s) 17, 51, 108, 168, 204, 289, 293, 403
SsiI CCGC 6 cut(s) 174, 389, 392, 432, 447, 464
SspMI CTAG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 2 cut(s) 108, 168
TaqI TCGA 2 cut(s) 26, 379
TatI WGTACW 1 cut(s) 213
TauI GCSGC 2 cut(s) 392, 467
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 3 cut(s) 230, 252, 282
Tru9I TTAA 3 cut(s) 230, 252, 282
TseI GCWGC 2 cut(s) 12, 269
TspDTI ATGAA 3 cut(s) 121, 230, 306
TspGWI ACGGA 1 cut(s) 78
XmiI GTMKAC 1 cut(s) 26
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.