Prupe.7G146200_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0014021
Physical Location & Seq
Forward (+)
15925856 .. 15926708
853 bp
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UTR
Exon/CDS
Intron
Prupe.7G146200.1

Sequence Viewer

Length: 480 bp
ATGGGTATTTGCAGCTCTTCCGGGTCGACCCAAGTGGCCACGGCCAAGCTGATCCTACAAGACGGGAAATTACAAGAATACCCGTACCCAGTAAAGGTTTCCTACGTGCTTCAAAAGAACCCGGCCTCCTTTATATGCAACTCGGACGAGATGGACTTCGACGACATCGTTCAGGCCGTTAATGAGAACGAGGAGCTCCAACCGGGCCAGCTCTACTTCGCTTTGCCACTCAGCTGGCTCAAGCATCCATTGCAGGCGGAGGAGATGGCCGCATTGGCCGTCAAAGCCAGCTCCGCGCTTATGAAGAGTGGGGGTGAGAAATGCGGCTGTCGCCGCAATTCGGTGTCGCAGGTGATGATATCCGGCCAGGAGGTCAAGGATCGCCGGCGAGTGGTCCCCGCCGGTGGTGCGTCGAGGACGGGGAGGGGTAGTGGAGGAAACAGACGGTGTGCGGCGTTGTTGAGTGCGATTCCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

160

Amino Acids

17.06

Weight (kDa)

8.29

Isoelectric Point (pI)

55.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23690
fragaria_vesca FvH4_1g14400
malus_domestica MD02G1157500.v1.1 MD15G1272100.v1.1
prunus_persica Prupe.7G146200_v2.0.a1
pyrus_communis pycom02g12450
rosa_chinensis RchiOBHm_Chr2g0103121
rosa_laevigata RLG00000017210
rosa_multiflora Rmu_sc0000388.1_g000007
rosa_roxburghii Rroxscaffold_2G00139910 Rroxscaffold_2G00140190
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160700 Rh2BG167400 Rh2CG167700 Rh2DG166300
rosa_wichuraiana Rw2G012580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
Acc36I ACCTGC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 26
AccII CGCG 1 cut(s) 296
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 7 cut(s) 257, 270, 294, 324, 334, 399, 452
AclWI GGATC 2 cut(s) 46, 387
AcoI YGGCCR 5 cut(s) 36, 42, 267, 276, 364
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 4 cut(s) 94, 340, 391, 404
AgsI TTSAA 1 cut(s) 113
AjnI CCWGG 1 cut(s) 366
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 6 cut(s) 15, 49, 196, 211, 234, 291
AluI AGCT 6 cut(s) 15, 49, 196, 211, 234, 291
Alw21I GWGCWC 1 cut(s) 198
AlwI GGATC 2 cut(s) 46, 387
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 8 cut(s) 36, 42, 123, 174, 205, 267, 276, 364
ApeKI GCWGC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 2 cut(s) 205, 394
AsuC2I CCSGG 3 cut(s) 22, 122, 204
AsuHPI GGTGA 2 cut(s) 326, 364
AvaII GGWCC 1 cut(s) 394
BalI TGGCCA 1 cut(s) 38
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 1 cut(s) 24
BccI CCATC 2 cut(s) 145, 259
BceAI ACGGC 3 cut(s) 57, 161, 263
BciT130I CCWGG 1 cut(s) 368
BcnI CCSGG 3 cut(s) 22, 122, 204
BfuAI ACCTGC 1 cut(s) 340
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 5 cut(s) 13, 270, 325, 334, 453
BlsI GCNGC 5 cut(s) 14, 271, 326, 335, 454
Bme1390I CCNGG 4 cut(s) 22, 122, 204, 368
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 2 cut(s) 205, 394
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 4 cut(s) 22, 122, 204, 368
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 253
BmuI ACTGGG 1 cut(s) 83
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 3 cut(s) 22, 122, 204
BsaAI YACGTR 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 39
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 415, 445
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 340, 391, 404
Bse118I RCCGGY 2 cut(s) 384, 401
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 248
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 4 cut(s) 94, 340, 391, 404
BseMI GCAATG 1 cut(s) 248
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 206, 275
BseXI GCAGC 1 cut(s) 24
Bsh1236I CGCG 1 cut(s) 296
BshFI GGCC 8 cut(s) 38, 44, 125, 176, 207, 269, 278, 366
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 7 cut(s) 21, 122, 203, 363, 385, 402, 473
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 4 cut(s) 94, 340, 391, 404
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 8 cut(s) 38, 44, 125, 176, 207, 269, 278, 366
Bsp1286I GDGCHC 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 2 cut(s) 51, 379
BspACI CCGC 7 cut(s) 257, 270, 294, 324, 334, 399, 452
BspANI GGCC 8 cut(s) 38, 44, 125, 176, 207, 269, 278, 366
BspCNI CTCAG 1 cut(s) 243
BspEI TCCGGA 1 cut(s) 472
BspFNI CGCG 1 cut(s) 296
BspLI GGNNCC 1 cut(s) 396
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 2 cut(s) 46, 387
BspQI GCTCTTC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 248
BsrFI RCCGGY 2 cut(s) 384, 401
BsrI ACTGG 1 cut(s) 89
BssAI RCCGGY 2 cut(s) 384, 401
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 2 cut(s) 51, 379
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 1 cut(s) 447
Bst6I CTCTTC 2 cut(s) 22, 299
BstAPI GCANNNNNTGC 1 cut(s) 250
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 5 cut(s) 209, 236, 255, 289, 386
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 2 cut(s) 54, 382
BstMBI GATC 2 cut(s) 51, 379
BstMWI GCNNNNNNNGC 7 cut(s) 250, 275, 284, 293, 330, 333, 407
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 4 cut(s) 20, 120, 202, 366
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 1 cut(s) 24
BstXI CCANNNNNNTGG 1 cut(s) 234
BsuRI GGCC 8 cut(s) 38, 44, 125, 176, 207, 269, 278, 366
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 244
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 5 cut(s) 209, 236, 255, 289, 386
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 2 cut(s) 384, 401
Cfr13I GGNCC 2 cut(s) 205, 394
CseI GACGC 1 cut(s) 399
Csp6I GTAC 1 cut(s) 85
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 230
DpnI GATC 2 cut(s) 53, 381
DpnII GATC 2 cut(s) 51, 379
EaeI YGGCCR 5 cut(s) 36, 42, 267, 276, 364
Eam1104I CTCTTC 2 cut(s) 22, 299
EarI CTCTTC 2 cut(s) 22, 299
EciI GGCGGA 1 cut(s) 272
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 366
EcoRV GATATC 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 198
FaiI YATR 3 cut(s) 134, 136, 302
FaqI GGGAC 1 cut(s) 380
FauI CCCGC 1 cut(s) 406
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 5 cut(s) 13, 270, 325, 334, 453
FokI GGATG 1 cut(s) 231
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 5 cut(s) 13, 270, 325, 334, 453
GlaI GCGC 1 cut(s) 297
GluI GCNGC 5 cut(s) 13, 270, 325, 334, 453
HaeIII GGCC 8 cut(s) 38, 44, 125, 176, 207, 269, 278, 366
HapII CCGG 7 cut(s) 21, 122, 203, 363, 385, 402, 473
HgaI GACGC 1 cut(s) 399
HhaI GCGC 1 cut(s) 298
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 1 cut(s) 469
HpaII CCGG 7 cut(s) 21, 122, 203, 363, 385, 402, 473
HphI GGTGA 2 cut(s) 326, 364
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 473
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 2 cut(s) 164, 415
HpyCH4III ACNGT 1 cut(s) 447
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 3 cut(s) 12, 138, 253
HpyF10VI GCNNNNNNNGC 7 cut(s) 250, 275, 284, 293, 330, 333, 407
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 1 cut(s) 105
HspAI GCGC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 472
KroI GCCGGC 1 cut(s) 384
KroNI GCCGGC 1 cut(s) 386
Kzo9I GATC 2 cut(s) 51, 379
LguI GCTCTTC 1 cut(s) 22
LmnI GCTCC 3 cut(s) 193, 201, 296
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 1 cut(s) 253
MaeII ACGT 1 cut(s) 105
MalI GATC 2 cut(s) 53, 381
MboI GATC 2 cut(s) 51, 379
MboII GAAGA 2 cut(s) 9, 316
MhlI GDGCHC 1 cut(s) 198
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 2 cut(s) 68, 337
MluNI TGGCCA 1 cut(s) 38
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 7 cut(s) 136, 184, 253, 364, 408, 417, 428
Mox20I TGGCCA 1 cut(s) 38
MreI CGCCGGCG 1 cut(s) 384
MroI TCCGGA 1 cut(s) 472
MroNI GCCGGC 1 cut(s) 384
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 180
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 7 cut(s) 21, 122, 203, 363, 385, 402, 473
MspR9I CCNGG 4 cut(s) 22, 122, 204, 368
MvaI CCWGG 1 cut(s) 368
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 7 cut(s) 250, 275, 284, 293, 330, 333, 407
NaeI GCCGGC 1 cut(s) 386
NciI CCSGG 3 cut(s) 22, 122, 204
NdeII GATC 2 cut(s) 51, 379
NgoMIV GCCGGC 1 cut(s) 384
NlaIV GGNNCC 1 cut(s) 396
PaqCI CACCTGC 1 cut(s) 340
PciSI GCTCTTC 1 cut(s) 22
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 174
PdiI GCCGGC 1 cut(s) 386
PfeI GAWTC 1 cut(s) 469
PkrI GCNGC 5 cut(s) 14, 271, 326, 335, 454
Ppu21I YACGTR 1 cut(s) 106
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 396
PspPI GGNCC 2 cut(s) 205, 394
PvuII CAGCTG 1 cut(s) 234
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SacI GAGCTC 1 cut(s) 198
SalI GTCGAC 1 cut(s) 25
SapI GCTCTTC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 5 cut(s) 13, 270, 325, 334, 453
Sau3AI GATC 2 cut(s) 51, 379
Sau96I GGNCC 2 cut(s) 205, 394
ScrFI CCNGG 4 cut(s) 22, 122, 204, 368
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 1 cut(s) 253
SfiI GGCCNNNNNGGCC 1 cut(s) 275
SgrAI CRCCGGYG 2 cut(s) 384, 401
SinI GGWCC 1 cut(s) 394
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 2 cut(s) 68, 337
SsiI CCGC 7 cut(s) 257, 270, 294, 324, 334, 399, 452
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 20, 120, 202, 366
TaaI ACNGT 1 cut(s) 447
TaiI ACGT 1 cut(s) 108
TaqI TCGA 3 cut(s) 26, 159, 413
TasI AATT 2 cut(s) 68, 337
TauI GCSGC 4 cut(s) 272, 327, 336, 455
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TseI GCWGC 1 cut(s) 12
TspDTI ATGAA 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.