FvH4_1g14400

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014021
Physical Location & Seq
Reverse (-)
8037577 .. 8038418
842 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14400.t1

Sequence Viewer

Length: 450 bp
ATGGGAATCTGCAGCTCCACCGGCTCCACCCAAGTGGCCACCGCCAAGCTGATCCTACCGGACGGCAAGCTTCAAGAGTACTCCTACCCCGTCCGGGTTTCCTACGTCCTCCAGAAGCACCCGGCTTGCTTTATATGCAACGCCGACGAGATGGACTTCGACGACGTCATTTCCGCCGTCAACGACGACGACGAGCTCCACCCGGGGCAGCTCTACTTCGCTTTGCCGATGAGCTGGCTCAAGCATCCCTTACAGGCCGAGGAGATGGCCGCATTGGCCGTCAAAGCCAGCTCCGCTCTCATGAAGAGCGGCGGCGAGAAATGCGCCTACAGGAAAAATTCCGTTTCTCCGGTGACTTTTTCCGGCGAGGAGGTCAAGTCTCGCCGGAGCGGCGGCGGAGCTAGGAGGGGGAGTAAGTTCTCCGCAATGTTGAGTGCAATACCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

150

Amino Acids

16.08

Weight (kDa)

6.3

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 139 8.6e-31 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23690
fragaria_vesca FvH4_1g14400
malus_domestica MD02G1157500.v1.1 MD15G1272100.v1.1
prunus_persica Prupe.7G146200_v2.0.a1
pyrus_communis pycom02g12450
rosa_chinensis RchiOBHm_Chr2g0103121
rosa_laevigata RLG00000017210
rosa_multiflora Rmu_sc0000388.1_g000007
rosa_roxburghii Rroxscaffold_2G00139910 Rroxscaffold_2G00140190
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160700 Rh2BG167400 Rh2CG167700 Rh2DG166300
rosa_wichuraiana Rw2G012580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AccBSI CCGCTC 3 cut(s) 296, 309, 390
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 3 cut(s) 36, 267, 276
AcsI RAATTY 1 cut(s) 337
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 1 cut(s) 74
AleI CACNNNNGTG 1 cut(s) 32
AluBI AGCT 8 cut(s) 15, 49, 70, 196, 211, 234, 291, 401
AluI AGCT 8 cut(s) 15, 49, 70, 196, 211, 234, 291, 401
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 4 cut(s) 36, 255, 267, 276
ApeKI GCWGC 2 cut(s) 12, 208
ApoI RAATTY 1 cut(s) 337
AspLEI GCGC 1 cut(s) 326
AsuC2I CCSGG 4 cut(s) 95, 122, 203, 204
AsuHPI GGTGA 1 cut(s) 364
AvaI CYCGRG 1 cut(s) 202
BalI TGGCCA 1 cut(s) 38
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 24, 220
BccI CCATC 2 cut(s) 145, 259
BceAI ACGGC 3 cut(s) 79, 161, 263
BcnI CCSGG 4 cut(s) 95, 122, 203, 204
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 1 cut(s) 402
BfmI CTRYAG 2 cut(s) 10, 328
BglI GCCNNNNNGGC 2 cut(s) 275, 390
BisI GCNGC 7 cut(s) 13, 209, 270, 310, 313, 391, 394
BlsI GCNGC 7 cut(s) 14, 210, 271, 311, 314, 392, 395
BmcAI AGTACT 1 cut(s) 80
Bme1390I CCNGG 4 cut(s) 95, 122, 203, 204
BmeT110I CYCGRG 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 4 cut(s) 95, 122, 203, 204
BmsI GCATC 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 4 cut(s) 95, 122, 203, 204
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 3 cut(s) 202, 203, 258
BsaWI WCCGGW 3 cut(s) 58, 349, 442
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 397, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse118I RCCGGY 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 432
BseDI CCNNGG 3 cut(s) 202, 203, 258
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 432
BseRI GAGGAG 2 cut(s) 275, 383
BseXI GCAGC 2 cut(s) 24, 220
BshFI GGCC 4 cut(s) 38, 257, 269, 278
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 9 cut(s) 21, 59, 94, 122, 203, 350, 363, 385, 443
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 384
BsnI GGCC 4 cut(s) 38, 257, 269, 278
BsoBI CYCGRG 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 1 cut(s) 51
BspANI GGCC 4 cut(s) 38, 257, 269, 278
BspHI TCATGA 1 cut(s) 300
BspLI GGNNCC 1 cut(s) 25
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 1 cut(s) 46
BspQI GCTCTTC 1 cut(s) 299
BsrBI CCGCTC 3 cut(s) 296, 309, 390
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 3 cut(s) 202, 203, 258
BssMI GATC 1 cut(s) 51
BssNI GRCGYC 1 cut(s) 165
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 4 cut(s) 68, 127, 236, 289
BstF5I GGATG 1 cut(s) 244
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 7 cut(s) 21, 135, 275, 284, 293, 321, 390
BstSCI CCNGG 4 cut(s) 93, 120, 201, 202
BstSFI CTRYAG 2 cut(s) 10, 328
BstV1I GCAGC 2 cut(s) 24, 220
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 4 cut(s) 38, 257, 269, 278
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 4 cut(s) 68, 127, 236, 289
CciI TCATGA 1 cut(s) 300
CfoI GCGC 1 cut(s) 326
Cfr10I RCCGGY 1 cut(s) 20
Cfr9I CCCGGG 1 cut(s) 202
Csp6I GTAC 1 cut(s) 79
CviAII CATG 1 cut(s) 301
CviQI GTAC 1 cut(s) 79
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EaeI YGGCCR 3 cut(s) 36, 267, 276
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
EciI GGCGGA 2 cut(s) 163, 411
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 196
Eco88I CYCGRG 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 198
FaeI CATG 1 cut(s) 304
FaiI YATR 3 cut(s) 134, 136, 302
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FatI CATG 1 cut(s) 300
Fnu4HI GCNGC 7 cut(s) 13, 209, 270, 310, 313, 391, 394
FokI GGATG 1 cut(s) 231
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 7 cut(s) 13, 209, 270, 310, 313, 391, 394
FspBI CTAG 1 cut(s) 402
GlaI GCGC 1 cut(s) 325
GluI GCNGC 7 cut(s) 13, 209, 270, 310, 313, 391, 394
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 4 cut(s) 38, 257, 269, 278
HapII CCGG 9 cut(s) 21, 59, 94, 122, 203, 350, 363, 385, 443
HhaI GCGC 1 cut(s) 326
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 1 cut(s) 304
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 1 cut(s) 6
HpaII CCGG 9 cut(s) 21, 59, 94, 122, 203, 350, 363, 385, 443
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 181
Hpy188III TCNNGA 3 cut(s) 74, 112, 301
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 6 cut(s) 149, 164, 167, 188, 191, 194
HpyCH4IV ACGT 2 cut(s) 105, 165
HpyCH4V TGCA 3 cut(s) 12, 138, 437
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 135, 275, 284, 293, 321, 390
HpySE526I ACGT 2 cut(s) 105, 165
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 1 cut(s) 304
HspAI GCGC 1 cut(s) 324
Kzo9I GATC 1 cut(s) 51
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 6 cut(s) 20, 29, 201, 296, 387, 398
Lsp1109I GCAGC 2 cut(s) 24, 220
LweI GCATC 1 cut(s) 253
MaeI CTAG 1 cut(s) 402
MaeII ACGT 2 cut(s) 105, 165
MaeIII GTNAC 1 cut(s) 352
MalI GATC 1 cut(s) 53
MbiI CCGCTC 3 cut(s) 296, 309, 390
MboI GATC 1 cut(s) 51
MboII GAAGA 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 198
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 1 cut(s) 337
MluNI TGGCCA 1 cut(s) 38
MnlI CCTC 5 cut(s) 119, 253, 361, 364, 399
Mox20I TGGCCA 1 cut(s) 38
MscI TGGCCA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 38
MspI CCGG 9 cut(s) 21, 59, 94, 122, 203, 350, 363, 385, 443
MspR9I CCNGG 4 cut(s) 95, 122, 203, 204
MwoI GCNNNNNNNGC 7 cut(s) 21, 135, 275, 284, 293, 321, 390
NciI CCSGG 4 cut(s) 95, 122, 203, 204
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 304
NlaIV GGNNCC 1 cut(s) 25
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 352
OliI CACNNNNGTG 1 cut(s) 32
PagI TCATGA 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 1 cut(s) 6
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 7 cut(s) 14, 210, 271, 311, 314, 392, 395
Psp124BI GAGCTC 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 25
PstI CTGCAG 1 cut(s) 14
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 32
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 299
SatI GCNGC 7 cut(s) 13, 209, 270, 310, 313, 391, 394
Sau3AI GATC 1 cut(s) 51
ScaI AGTACT 1 cut(s) 80
ScrFI CCNGG 4 cut(s) 95, 122, 203, 204
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 2 cut(s) 10, 328
SfiI GGCCNNNNNGGCC 1 cut(s) 275
SmaI CCCGGG 1 cut(s) 204
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 337
SspMI CTAG 1 cut(s) 402
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 93, 120, 201, 202
TaiI ACGT 2 cut(s) 108, 168
TaqI TCGA 1 cut(s) 159
TasI AATT 1 cut(s) 337
TatI WGTACW 1 cut(s) 78
TauI GCSGC 5 cut(s) 272, 312, 315, 393, 396
TfiI GAWTC 1 cut(s) 6
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 12, 208
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 317
TspGWI ACGGA 1 cut(s) 331
TspMI CCCGGG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 164
XapI RAATTY 1 cut(s) 337
XmaI CCCGGG 1 cut(s) 202
XspI CTAG 1 cut(s) 402
ZraI GACGTC 1 cut(s) 166
ZrmI AGTACT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.