MD15G1272100.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014021
Physical Location & Seq
Reverse (-)
23778227 .. 23778712
486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1272100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGGTATTTGCAGCTCGTCCGGGTCGACCCAAGTGGCCACCGCCAAGCTGATTCTACAGGACGGAAAATTGCAAGAATATCCGTATCCCGTAAAGGTCTCCTACGTGCTCCAAAAGAACCCCACCTGCTTCATTTGCAACTCCGACGATATGGACTTTGACGACGTCCTTCAGGCCGTCAGCGGTAACGAGGTGCTGCAGCCCGGCCAGCTGTACTTTGCCTTGCCACTCAGCTGGCTCAAGCACCCGCTAAAGGCGGAGGAGATGGCCGCATTGGCCGTTAAAGCCAGCTCCGCGCTTATGAAGAGTGGCGGCGAGAAATGTGGGTGCCGCCGGAATTCGGTGTCGCCGCTGATATTTTCCGGCGAGGACGTGAAGGATCGTCGGAGAGTGGTAACAGCTGGTGCCAGTGGGGGGTCGAGGAGGGGGAGGGGAAATGGGGGTAAAAGGCGGTACGCGGCGGACTTGAGTGCGATTCCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

162

Amino Acids

17.26

Weight (kDa)

9.01

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 161 5.7e-28 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23690
fragaria_vesca FvH4_1g14400
malus_domestica MD02G1157500.v1.1 MD15G1272100.v1.1
prunus_persica Prupe.7G146200_v2.0.a1
pyrus_communis pycom02g12450
rosa_chinensis RchiOBHm_Chr2g0103121
rosa_laevigata RLG00000017210
rosa_multiflora Rmu_sc0000388.1_g000007
rosa_roxburghii Rroxscaffold_2G00139910 Rroxscaffold_2G00140190
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160700 Rh2BG167400 Rh2CG167700 Rh2DG166300
rosa_wichuraiana Rw2G012580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 134
AatII GACGTC 1 cut(s) 168
Acc36I ACCTGC 1 cut(s) 134
AccB1I GGYRCC 2 cut(s) 327, 404
AccI GTMKAC 1 cut(s) 26
AccII CGCG 2 cut(s) 296, 458
AccIII TCCGGA 1 cut(s) 478
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 4 cut(s) 36, 205, 267, 276
AcsI RAATTY 1 cut(s) 337
AcuI CTGAAG 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 2 cut(s) 215, 455
AfiI CCNNNNNNNGG 3 cut(s) 253, 340, 414
AjiI CACGTC 1 cut(s) 373
AluBI AGCT 6 cut(s) 15, 49, 211, 234, 291, 401
AluI AGCT 6 cut(s) 15, 49, 211, 234, 291, 401
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 1 cut(s) 387
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 5 cut(s) 36, 174, 205, 267, 276
ApeKI GCWGC 3 cut(s) 12, 196, 199
ApoI RAATTY 1 cut(s) 337
AspLEI GCGC 1 cut(s) 298
AsuC2I CCSGG 2 cut(s) 22, 204
BalI TGGCCA 1 cut(s) 38
BanI GGYRCC 2 cut(s) 327, 404
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 3 cut(s) 24, 183, 211
BccI CCATC 1 cut(s) 259
BceAI ACGGC 2 cut(s) 161, 263
BciVI GTATCC 1 cut(s) 96
BcnI CCSGG 2 cut(s) 22, 204
BcoDI GTCTC 1 cut(s) 103
BfmI CTRYAG 2 cut(s) 56, 197
BfuAI ACCTGC 1 cut(s) 134
BfuI GTATCC 1 cut(s) 96
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 8 cut(s) 13, 197, 200, 270, 313, 331, 350, 459
BlsI GCNGC 8 cut(s) 14, 198, 201, 271, 314, 332, 351, 460
Bme1390I CCNGG 2 cut(s) 22, 204
BmgBI CACGTC 1 cut(s) 373
BmiI GGNNCC 2 cut(s) 329, 406
BmrFI CCNGG 2 cut(s) 22, 204
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 2 cut(s) 22, 204
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 103
BsaWI WCCGGW 1 cut(s) 478
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 340, 414
Bse1I ACTGG 1 cut(s) 408
BseAI TCCGGA 1 cut(s) 478
BseLI CCNNNNNNNGG 3 cut(s) 253, 340, 414
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 2 cut(s) 275, 436
BseXI GCAGC 3 cut(s) 24, 183, 211
Bsh1236I CGCG 2 cut(s) 296, 458
BshFI GGCC 5 cut(s) 38, 176, 207, 269, 278
BshNI GGYRCC 2 cut(s) 327, 404
BsiHKAI GWGCWC 1 cut(s) 111
BsiSI CCGG 5 cut(s) 21, 204, 334, 363, 479
BslI CCNNNNNNNGG 3 cut(s) 253, 340, 414
BsmAI GTCTC 1 cut(s) 103
BsnI GGCC 5 cut(s) 38, 176, 207, 269, 278
Bso31I GGTCTC 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 111
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 1 cut(s) 379
BspANI GGCC 5 cut(s) 38, 176, 207, 269, 278
BspCNI CTCAG 1 cut(s) 243
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 2 cut(s) 296, 458
BspLI GGNNCC 2 cut(s) 329, 406
BspMAI CTGCAG 1 cut(s) 201
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 1 cut(s) 387
BspT107I GGYRCC 2 cut(s) 327, 404
BspTNI GGTCTC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 408
BssMI GATC 1 cut(s) 379
BssNI GRCGYC 1 cut(s) 165
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 3 cut(s) 209, 236, 289
BstDEI CTNAG 1 cut(s) 230
BstFNI CGCG 2 cut(s) 296, 458
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 5 cut(s) 135, 208, 275, 284, 293
BstSCI CCNGG 2 cut(s) 20, 202
BstSFI CTRYAG 2 cut(s) 56, 197
BstUI CGCG 2 cut(s) 296, 458
BstV1I GCAGC 3 cut(s) 24, 183, 211
BstXI CCANNNNNNTGG 1 cut(s) 234
BsuI GTATCC 1 cut(s) 96
BsuRI GGCC 5 cut(s) 38, 176, 207, 269, 278
BtrI CACGTC 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 415
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 3 cut(s) 209, 236, 289
CfoI GCGC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 214, 454
CviQI GTAC 2 cut(s) 214, 454
DdeI CTNAG 1 cut(s) 230
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
EaeI YGGCCR 4 cut(s) 36, 205, 267, 276
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
EciI GGCGGA 2 cut(s) 272, 476
Eco31I GGTCTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 155
EcoRI GAATTC 1 cut(s) 337
FaiI YATR 2 cut(s) 152, 302
FauI CCCGC 1 cut(s) 255
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 8 cut(s) 13, 197, 200, 270, 313, 331, 350, 459
Fsp4HI GCNGC 8 cut(s) 13, 197, 200, 270, 313, 331, 350, 459
GlaI GCGC 1 cut(s) 297
GluI GCNGC 8 cut(s) 13, 197, 200, 270, 313, 331, 350, 459
HaeIII GGCC 5 cut(s) 38, 176, 207, 269, 278
HapII CCGG 5 cut(s) 21, 204, 334, 363, 479
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 165
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 2 cut(s) 52, 475
HpaII CCGG 5 cut(s) 21, 204, 334, 363, 479
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 2 cut(s) 145, 387
Hpy188III TCNNGA 1 cut(s) 479
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 3 cut(s) 149, 167, 387
HpyAV CCTTC 2 cut(s) 179, 370
HpyCH4IV ACGT 3 cut(s) 105, 165, 372
HpyCH4V TGCA 4 cut(s) 12, 73, 138, 199
HpyF10VI GCNNNNNNNGC 5 cut(s) 135, 208, 275, 284, 293
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 3 cut(s) 105, 165, 372
Hsp92I GRCGYC 1 cut(s) 165
HspAI GCGC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 478
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 2 cut(s) 114, 296
Lsp1109I GCAGC 3 cut(s) 24, 183, 211
MaeII ACGT 3 cut(s) 105, 165, 372
MaeIII GTNAC 2 cut(s) 185, 394
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 111
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 2 cut(s) 68, 337
MluNI TGGCCA 1 cut(s) 38
MmeI TCCRAC 2 cut(s) 168, 365
MnlI CCTC 6 cut(s) 184, 253, 361, 414, 417, 423
Mox20I TGGCCA 1 cut(s) 38
MroI TCCGGA 1 cut(s) 478
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 5 cut(s) 183, 211, 234, 352, 401
MspI CCGG 5 cut(s) 21, 204, 334, 363, 479
MspR9I CCNGG 2 cut(s) 22, 204
MvnI CGCG 2 cut(s) 296, 458
MwoI GCNNNNNNNGC 5 cut(s) 135, 208, 275, 284, 293
NciI CCSGG 2 cut(s) 22, 204
NdeII GATC 1 cut(s) 379
NlaIV GGNNCC 2 cut(s) 329, 406
PaqCI CACCTGC 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PfeI GAWTC 2 cut(s) 52, 475
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 8 cut(s) 14, 198, 201, 271, 314, 332, 351, 460
Ppu21I YACGTR 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 329, 406
PstI CTGCAG 1 cut(s) 201
PsyI GACNNNGTC 1 cut(s) 164
PvuII CAGCTG 3 cut(s) 211, 234, 401
RsaI GTAC 2 cut(s) 215, 455
RsaNI GTAC 2 cut(s) 214, 454
SalI GTCGAC 1 cut(s) 25
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 8 cut(s) 13, 197, 200, 270, 313, 331, 350, 459
Sau3AI GATC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 22, 204
SduI GDGCHC 1 cut(s) 111
SfcI CTRYAG 2 cut(s) 56, 197
SfiI GGCCNNNNNGGCC 1 cut(s) 275
SmlI CTYRAG 2 cut(s) 239, 466
SmoI CTYRAG 2 cut(s) 239, 466
Sse9I AATT 2 cut(s) 68, 337
StyD4I CCNGG 2 cut(s) 20, 202
TaiI ACGT 3 cut(s) 108, 168, 375
TaqI TCGA 2 cut(s) 26, 419
TasI AATT 2 cut(s) 68, 337
TatI WGTACW 1 cut(s) 213
TauI GCSGC 5 cut(s) 272, 315, 333, 352, 461
TfiI GAWTC 2 cut(s) 52, 475
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 415
TseI GCWGC 3 cut(s) 12, 196, 199
TspDTI ATGAA 2 cut(s) 121, 317
TspGWI ACGGA 2 cut(s) 72, 78
TspRI CASTG 1 cut(s) 415
Tth111I GACNNNGTC 1 cut(s) 164
XapI RAATTY 1 cut(s) 337
XmiI GTMKAC 1 cut(s) 26
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.