Rroxscaffold_2G00140190

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014021
Physical Location & Seq
Reverse (-)
78434707 .. 78435773
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140190.1

Sequence Viewer

Length: 447 bp
ATGGGAATCTGCAGCTCCACGAGCTCGACCCAAGTGGCCACCGCCAAGCTTATTCTACCCGACGGCAAACTACAGGAGTACTCCTACCCCGTCCGGGTTTCCTACGTCCTCCAGAAGCACCCCGATTGCTTTATATGCAACGCCGACGAGATGGACTTCGACGACGTCGTTTCCGCCGTCAACGACAACGACGAGCTCCACCCGGGGCAGCTCTACTTCGCTTTGCCGACGAGCTGGCTCAAGCTTCCCTTACAGGCCGAGGAAATGGCCGCATTGGCCGTCAGAGCCAGCTCCGCTCTCATGCAGAGAGGCGGCGAGAAATGCGGCTTTCGAAAAAATTCCGTTTCTCCGGTGACTTTTTCCGGCGAGGATGTGAAGTCCGGCCGGACCACCGGCGGGGCGAGGAGGGGGAGTGGTAGTGGGGGAGGAAGTTCTCGGCAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

148

Amino Acids

15.8

Weight (kDa)

6.09

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 135 1.8e-27 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23690
fragaria_vesca FvH4_1g14400
malus_domestica MD02G1157500.v1.1 MD15G1272100.v1.1
prunus_persica Prupe.7G146200_v2.0.a1
pyrus_communis pycom02g12450
rosa_chinensis RchiOBHm_Chr2g0103121
rosa_laevigata RLG00000017210
rosa_multiflora Rmu_sc0000388.1_g000007
rosa_roxburghii Rroxscaffold_2G00139910 Rroxscaffold_2G00140190
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160700 Rh2BG167400 Rh2CG167700 Rh2DG166300
rosa_wichuraiana Rw2G012580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 296
AciI CCGC 7 cut(s) 42, 174, 270, 294, 312, 324, 396
AcoI YGGCCR 4 cut(s) 36, 267, 276, 382
AcsI RAATTY 1 cut(s) 337
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 94, 396
AluBI AGCT 8 cut(s) 15, 24, 49, 196, 211, 234, 244, 291
AluI AGCT 8 cut(s) 15, 24, 49, 196, 211, 234, 244, 291
Alw21I GWGCWC 2 cut(s) 26, 198
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 5 cut(s) 36, 255, 267, 276, 382
ApeKI GCWGC 2 cut(s) 12, 208
ApoI RAATTY 1 cut(s) 337
Asp700I GAANNNNTTC 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 387
AsuC2I CCSGG 3 cut(s) 95, 203, 204
AsuHPI GGTGA 1 cut(s) 364
AsuII TTCGAA 1 cut(s) 331
AvaI CYCGRG 1 cut(s) 202
AvaII GGWCC 1 cut(s) 387
BalI TGGCCA 1 cut(s) 38
BanII GRGCYC 2 cut(s) 26, 198
BauI CACGAG 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 26, 198
BbvI GCAGC 2 cut(s) 24, 220
BccI CCATC 1 cut(s) 145
BceAI ACGGC 3 cut(s) 79, 161, 263
BcnI CCSGG 3 cut(s) 95, 203, 204
BfmI CTRYAG 2 cut(s) 10, 71
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 5 cut(s) 13, 209, 270, 313, 325
BlsI GCNGC 5 cut(s) 14, 210, 271, 314, 326
BmcAI AGTACT 1 cut(s) 80
Bme1390I CCNGG 3 cut(s) 95, 203, 204
Bme18I GGWCC 1 cut(s) 387
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 387
BmrFI CCNGG 3 cut(s) 95, 203, 204
BpmI CTGGAG 1 cut(s) 95
Bpu14I TTCGAA 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 3 cut(s) 95, 203, 204
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 3 cut(s) 202, 203, 258
BsaWI WCCGGW 1 cut(s) 349
BsaXI ACNNNNNCTCC 4 cut(s) 397, 403, 427, 433
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 396
Bse118I RCCGGY 1 cut(s) 392
Bse3DI GCAATG 1 cut(s) 446
BseDI CCNNGG 3 cut(s) 202, 203, 258
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 2 cut(s) 94, 396
BseMI GCAATG 1 cut(s) 446
BseRI GAGGAG 1 cut(s) 418
BseX3I CGGCCG 1 cut(s) 382
BseXI GCAGC 2 cut(s) 24, 220
Bsh1285I CGRYCG 1 cut(s) 385
BshFI GGCC 5 cut(s) 38, 257, 269, 278, 384
BsiEI CGRYCG 1 cut(s) 385
BsiHKAI GWGCWC 2 cut(s) 26, 198
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 7 cut(s) 94, 203, 350, 363, 381, 385, 393
BslI CCNNNNNNNGG 2 cut(s) 94, 396
BsnI GGCC 5 cut(s) 38, 257, 269, 278, 384
BsoBI CYCGRG 1 cut(s) 202
Bsp119I TTCGAA 1 cut(s) 331
Bsp1286I GDGCHC 2 cut(s) 26, 198
BspACI CCGC 7 cut(s) 42, 174, 270, 294, 312, 324, 396
BspANI GGCC 5 cut(s) 38, 257, 269, 278, 384
BspMAI CTGCAG 1 cut(s) 14
BspT104I TTCGAA 1 cut(s) 331
BsrBI CCGCTC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 392
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 3 cut(s) 202, 203, 258
BssNI GRCGYC 1 cut(s) 165
BssSI CACGAG 1 cut(s) 19
Bst2BI CACGAG 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 165
BstBI TTCGAA 1 cut(s) 331
BstC8I GCNNGC 2 cut(s) 236, 289
BstF5I GGATG 1 cut(s) 376
BstMCI CGRYCG 1 cut(s) 385
BstMWI GCNNNNNNNGC 6 cut(s) 21, 135, 275, 284, 293, 321
BstSCI CCNGG 3 cut(s) 93, 201, 202
BstSFI CTRYAG 2 cut(s) 10, 71
BstV1I GCAGC 2 cut(s) 24, 220
BstZI CGGCCG 1 cut(s) 382
BsuRI GGCC 5 cut(s) 38, 257, 269, 278, 384
BtsCI GGATG 1 cut(s) 376
Cac8I GCNNGC 2 cut(s) 236, 289
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 387
Cfr9I CCCGGG 1 cut(s) 202
Csp6I GTAC 1 cut(s) 79
CviAII CATG 1 cut(s) 301
CviQI GTAC 1 cut(s) 79
EaeI YGGCCR 4 cut(s) 36, 267, 276, 382
EagI CGGCCG 1 cut(s) 382
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 2 cut(s) 24, 196
EclXI CGGCCG 1 cut(s) 382
Eco24I GRGCYC 2 cut(s) 26, 198
Eco47I GGWCC 1 cut(s) 387
Eco52I CGGCCG 1 cut(s) 382
Eco53kI GAGCTC 2 cut(s) 24, 196
Eco88I CYCGRG 1 cut(s) 202
EcoICRI GAGCTC 2 cut(s) 24, 196
EcoT38I GRGCYC 2 cut(s) 26, 198
FaeI CATG 1 cut(s) 304
FaiI YATR 3 cut(s) 134, 136, 302
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FatI CATG 1 cut(s) 300
FauI CCCGC 1 cut(s) 389
Fnu4HI GCNGC 5 cut(s) 13, 209, 270, 313, 325
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 2 cut(s) 26, 198
Fsp4HI GCNGC 5 cut(s) 13, 209, 270, 313, 325
GluI GCNGC 5 cut(s) 13, 209, 270, 313, 325
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 5 cut(s) 38, 257, 269, 278, 384
HapII CCGG 7 cut(s) 94, 203, 350, 363, 381, 385, 393
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 1 cut(s) 304
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 2 cut(s) 47, 242
HinfI GANTC 1 cut(s) 6
HpaII CCGG 7 cut(s) 94, 203, 350, 363, 381, 385, 393
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 1 cut(s) 284
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 7 cut(s) 65, 149, 164, 167, 170, 194, 232
HpyCH4IV ACGT 2 cut(s) 105, 165
HpyCH4V TGCA 3 cut(s) 12, 138, 304
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 135, 275, 284, 293, 321
HpySE526I ACGT 2 cut(s) 105, 165
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 1 cut(s) 304
LmnI GCTCC 3 cut(s) 20, 201, 296
Lsp1109I GCAGC 2 cut(s) 24, 220
MaeII ACGT 2 cut(s) 105, 165
MaeIII GTNAC 1 cut(s) 352
MbiI CCGCTC 1 cut(s) 296
MhlI GDGCHC 2 cut(s) 26, 198
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 1 cut(s) 337
MluNI TGGCCA 1 cut(s) 38
MnlI CCTC 7 cut(s) 119, 253, 302, 361, 396, 399, 419
Mox20I TGGCCA 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 337
MscI TGGCCA 1 cut(s) 38
Msp20I TGGCCA 1 cut(s) 38
MspI CCGG 7 cut(s) 94, 203, 350, 363, 381, 385, 393
MspR9I CCNGG 3 cut(s) 95, 203, 204
MwoI GCNNNNNNNGC 6 cut(s) 21, 135, 275, 284, 293, 321
NciI CCSGG 3 cut(s) 95, 203, 204
NlaIII CATG 1 cut(s) 304
NmeAIII GCCGAG 2 cut(s) 283, 415
NmuCI GTSAC 1 cut(s) 352
NspV TTCGAA 1 cut(s) 331
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 174, 189
PdmI GAANNNNTTC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 6
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 5 cut(s) 14, 210, 271, 314, 326
Psp124BI GAGCTC 2 cut(s) 26, 198
PspPI GGNCC 1 cut(s) 387
PstI CTGCAG 1 cut(s) 14
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SacI GAGCTC 2 cut(s) 26, 198
SatI GCNGC 5 cut(s) 13, 209, 270, 313, 325
Sau96I GGNCC 1 cut(s) 387
ScaI AGTACT 1 cut(s) 80
ScrFI CCNGG 3 cut(s) 95, 203, 204
SduI GDGCHC 2 cut(s) 26, 198
SfcI CTRYAG 2 cut(s) 10, 71
SfiI GGCCNNNNNGGCC 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 331
SgrAI CRCCGGYG 1 cut(s) 392
SinI GGWCC 1 cut(s) 387
SmaI CCCGGG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 337
SsiI CCGC 7 cut(s) 42, 174, 270, 294, 312, 324, 396
SstI GAGCTC 2 cut(s) 26, 198
StyD4I CCNGG 3 cut(s) 93, 201, 202
TaiI ACGT 2 cut(s) 108, 168
TaqI TCGA 3 cut(s) 26, 159, 331
TasI AATT 1 cut(s) 337
TatI WGTACW 1 cut(s) 78
TauI GCSGC 3 cut(s) 272, 315, 327
TfiI GAWTC 1 cut(s) 6
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 12, 208
Tsp45I GTSAC 1 cut(s) 352
TspGWI ACGGA 1 cut(s) 331
TspMI CCCGGG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 387
XapI RAATTY 1 cut(s) 337
XmaI CCCGGG 1 cut(s) 202
XmnI GAANNNNTTC 1 cut(s) 337
ZraI GACGTC 1 cut(s) 166
ZrmI AGTACT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.