MD02G1157500.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014021
Physical Location & Seq
Reverse (-)
13174491 .. 13174967
477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1157500.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGGTATTTGCAGCTCGTCCGGGTCGACCCAAGTGGCCACCGCCAAGCTGATTCTACAGGACGGAAATTTACAAGAATTCCCGTACCCGGTAAAGGTTTCCTACGTGCTCCAAAAGAACCCCACTTGCTTCATCTGCAACTCCGACGAGATGGACTTCGACGACGTCCTTCAGGCCGTCAGTGATAACGAGGTGCTCCAGCCGGGACAGCTCTACTTTGCCTTGCCGCTCAGCTGGCTCAAGCACCCGCTGCAAGCGGAGGAGATGGCCGCATTGGCCGTAAAAGCCAGCTCCGCGCTTATGAAGAGTGGCGGCGAAAAATGTGGATGTCGCCGGAATTCGGTGTCTCCACTGATATTTTCCGGCGTGGAGGTGAAGGATCGCCGGAGAGTGGTGGCCAGTGGGGGGTCGAGGAGGCGGAGGGATAGTGGTGGAAATGGACGGTTTGCGGCGGAGTTGAGTGCAATTCCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

159

Amino Acids

17.07

Weight (kDa)

7.6

Isoelectric Point (pI)

63.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 158 3e-27 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23690
fragaria_vesca FvH4_1g14400
malus_domestica MD02G1157500.v1.1 MD15G1272100.v1.1
prunus_persica Prupe.7G146200_v2.0.a1
pyrus_communis pycom02g12450
rosa_chinensis RchiOBHm_Chr2g0103121
rosa_laevigata RLG00000017210
rosa_multiflora Rmu_sc0000388.1_g000007
rosa_roxburghii Rroxscaffold_2G00139910 Rroxscaffold_2G00140190
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160700 Rh2BG167400 Rh2CG167700 Rh2DG166300
rosa_wichuraiana Rw2G012580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 26
AccII CGCG 1 cut(s) 296
AccIII TCCGGA 1 cut(s) 469
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 4 cut(s) 36, 267, 276, 396
AcsI RAATTY 3 cut(s) 67, 77, 337
AcuI CTGAAG 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 5 cut(s) 88, 94, 340, 391, 405
AluBI AGCT 5 cut(s) 15, 49, 211, 234, 291
AluI AGCT 5 cut(s) 15, 49, 211, 234, 291
Alw21I GWGCWC 2 cut(s) 111, 198
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 1 cut(s) 387
Aor13HI TCCGGA 1 cut(s) 469
AoxI GGCC 5 cut(s) 36, 174, 267, 276, 396
ApeKI GCWGC 2 cut(s) 12, 250
ApoI RAATTY 3 cut(s) 67, 77, 337
AspLEI GCGC 1 cut(s) 298
AsuC2I CCSGG 3 cut(s) 22, 89, 204
AsuHPI GGTGA 1 cut(s) 385
BalI TGGCCA 2 cut(s) 38, 398
Bbv12I GWGCWC 2 cut(s) 111, 198
BbvI GCAGC 2 cut(s) 24, 237
BccI CCATC 2 cut(s) 145, 259
BceAI ACGGC 2 cut(s) 161, 263
BcnI CCSGG 3 cut(s) 22, 89, 204
BcoDI GTCTC 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 56
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 6 cut(s) 13, 227, 251, 270, 313, 450
BlpI GCTNAGC 1 cut(s) 230
BlsI GCNGC 6 cut(s) 14, 228, 252, 271, 314, 451
Bme1390I CCNGG 3 cut(s) 22, 89, 204
BmrFI CCNGG 3 cut(s) 22, 89, 204
BpmI CTGGAG 1 cut(s) 182
Bpu1102I GCTNAGC 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 3 cut(s) 22, 89, 204
BsaAI YACGTR 1 cut(s) 106
BsaHI GRCGYC 1 cut(s) 165
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 94, 340, 391, 405
Bse1I ACTGG 1 cut(s) 399
BseAI TCCGGA 1 cut(s) 469
BseGI GGATG 1 cut(s) 332
BseLI CCNNNNNNNGG 5 cut(s) 88, 94, 340, 391, 405
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 399
BseRI GAGGAG 2 cut(s) 275, 427
BseXI GCAGC 2 cut(s) 24, 237
Bsh1236I CGCG 1 cut(s) 296
BshFI GGCC 5 cut(s) 38, 176, 269, 278, 398
BsiHKAI GWGCWC 2 cut(s) 111, 198
BsiSI CCGG 7 cut(s) 21, 89, 203, 334, 363, 385, 470
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 5 cut(s) 88, 94, 340, 391, 405
BsmAI GTCTC 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 5 cut(s) 38, 176, 269, 278, 398
Bsp1286I GDGCHC 2 cut(s) 111, 198
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 1 cut(s) 379
Bsp1720I GCTNAGC 1 cut(s) 230
BspANI GGCC 5 cut(s) 38, 176, 269, 278, 398
BspCNI CTCAG 1 cut(s) 243
BspEI TCCGGA 1 cut(s) 469
BspFNI CGCG 1 cut(s) 296
BspPI GGATC 1 cut(s) 387
BsrBI CCGCTC 1 cut(s) 229
BsrI ACTGG 1 cut(s) 399
BssMI GATC 1 cut(s) 379
BssNI GRCGYC 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 444
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 165
BstAPI GCANNNNNTGC 1 cut(s) 250
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 3 cut(s) 236, 255, 289
BstDEI CTNAG 1 cut(s) 230
BstF5I GGATG 1 cut(s) 332
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 7 cut(s) 135, 208, 235, 250, 275, 284, 293
BstSCI CCNGG 3 cut(s) 20, 87, 202
BstSFI CTRYAG 1 cut(s) 56
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 2 cut(s) 24, 237
BsuRI GGCC 5 cut(s) 38, 176, 269, 278, 398
BtsCI GGATG 1 cut(s) 332
BtsIMutI CAGTG 3 cut(s) 187, 350, 406
Cac8I GCNNGC 3 cut(s) 236, 255, 289
CfoI GCGC 1 cut(s) 298
Csp6I GTAC 1 cut(s) 85
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 230
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
EaeI YGGCCR 4 cut(s) 36, 267, 276, 396
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
EciI GGCGGA 2 cut(s) 433, 467
Eco57I CTGAAG 1 cut(s) 155
EcoRI GAATTC 2 cut(s) 77, 337
FaiI YATR 1 cut(s) 302
FaqI GGGAC 1 cut(s) 219
FauI CCCGC 1 cut(s) 255
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 6 cut(s) 13, 227, 251, 270, 313, 450
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 6 cut(s) 13, 227, 251, 270, 313, 450
GlaI GCGC 1 cut(s) 297
GluI GCNGC 6 cut(s) 13, 227, 251, 270, 313, 450
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 5 cut(s) 38, 176, 269, 278, 398
HapII CCGG 7 cut(s) 21, 89, 203, 334, 363, 385, 470
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 165
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 1 cut(s) 52
HpaII CCGG 7 cut(s) 21, 89, 203, 334, 363, 385, 470
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 3 cut(s) 149, 164, 167
HpyAV CCTTC 2 cut(s) 179, 370
HpyCH4III ACNGT 1 cut(s) 444
HpyCH4IV ACGT 2 cut(s) 105, 165
HpyCH4V TGCA 4 cut(s) 12, 138, 253, 464
HpyF10VI GCNNNNNNNGC 7 cut(s) 135, 208, 235, 250, 275, 284, 293
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 2 cut(s) 105, 165
Hsp92I GRCGYC 1 cut(s) 165
HspAI GCGC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 469
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 3 cut(s) 114, 201, 296
Lsp1109I GCAGC 2 cut(s) 24, 237
MaeII ACGT 2 cut(s) 105, 165
MalI GATC 1 cut(s) 381
MbiI CCGCTC 1 cut(s) 229
MboI GATC 1 cut(s) 379
MboII GAAGA 1 cut(s) 316
MhlI GDGCHC 2 cut(s) 111, 198
MlsI TGGCCA 2 cut(s) 38, 398
MluCI AATT 4 cut(s) 67, 77, 337, 465
MluNI TGGCCA 2 cut(s) 38, 398
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 6 cut(s) 184, 253, 364, 405, 408, 414
Mox20I TGGCCA 2 cut(s) 38, 398
MroI TCCGGA 1 cut(s) 469
MscI TGGCCA 2 cut(s) 38, 398
Msp20I TGGCCA 2 cut(s) 38, 398
MspA1I CMGCKG 2 cut(s) 234, 250
MspI CCGG 7 cut(s) 21, 89, 203, 334, 363, 385, 470
MspR9I CCNGG 3 cut(s) 22, 89, 204
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 7 cut(s) 135, 208, 235, 250, 275, 284, 293
NciI CCSGG 3 cut(s) 22, 89, 204
NdeII GATC 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PfeI GAWTC 1 cut(s) 52
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 6 cut(s) 14, 228, 252, 271, 314, 451
Ppu21I YACGTR 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 234
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SalI GTCGAC 1 cut(s) 25
SatI GCNGC 6 cut(s) 13, 227, 251, 270, 313, 450
Sau3AI GATC 1 cut(s) 379
ScrFI CCNGG 3 cut(s) 22, 89, 204
SduI GDGCHC 2 cut(s) 111, 198
SfcI CTRYAG 1 cut(s) 56
SfiI GGCCNNNNNGGCC 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 4 cut(s) 67, 77, 337, 465
StyD4I CCNGG 3 cut(s) 20, 87, 202
TaaI ACNGT 1 cut(s) 444
TaiI ACGT 2 cut(s) 108, 168
TaqI TCGA 3 cut(s) 26, 159, 410
TasI AATT 4 cut(s) 67, 77, 337, 465
TauI GCSGC 4 cut(s) 229, 272, 315, 452
TfiI GAWTC 1 cut(s) 52
TscAI CASTG 3 cut(s) 187, 357, 406
TseI GCWGC 2 cut(s) 12, 250
TspDTI ATGAA 2 cut(s) 121, 317
TspGWI ACGGA 1 cut(s) 78
TspRI CASTG 3 cut(s) 187, 357, 406
Tth111I GACNNNGTC 1 cut(s) 164
XapI RAATTY 3 cut(s) 67, 77, 337
XmiI GTMKAC 1 cut(s) 26
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.