Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003178

Physical Location & Seq

Genomic Coordinates Reverse (-)

10653683 .. 10660558

Gene Structure 6876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G25050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3507 bp

ATGGCGTTGTTTAGAAAGTTCTTTCACAGAAAACCTCCTGAAGGGTTGCTTGAGATCTCAGAGAGAGTTTACGTCTTTGATTGCTGCTTAACCACGGATATGTTGGAAGATGAAGATTACAGAGTTTATGTGAGCCGCATAATGAGTCAGTTAAGGGAACAGTTTCCCGGTGCATCATTCATGGTGTTTAATTTCCGGGATGGAGATAGCAGAAGCCGGATGGAGAGTGTACTAACTGAGTATGACATGACCATCATGGACTATCCTCGCCATTATGAAGGTTGCCCTTTGCTTACAATGGAAACGGTGCATCACTTCCTAAAATCAGCTGAAAGTTGGTTATTGTTAAGTCAACAGAATATCTTATTGTCGCACTGTGAGCTTGGCGGATGGCCGACTTTAGCTTTTATGCTTGCTTCGCTCTTGTTATACCGCAAACAGTTTAGTGGAGAACACAGAACACTAGAAATGATATATAAGCAGGCTCCTCGTGAGTTGTTACAGCTAATGTCTCCACTAAATCCGCTGCCTTCACAGTTGAGGTTTCTTCAGTATATCTCGAGGAGGAATGTTGGCTCACAATGGCCACCGCTAGACCAGGCCCTTACATTAGACTGTGTTAATCTTAGACTGATTCCTGATTTTGATGGTGAGGGTGGTTGCCGACCCATATTCAGAATATATGGTCAAGATCCTTTTATGGCTTCTGATCGGACTTCAAAAGTTCTTTTCTCGATGCCTAAAAGAAGCAAAGCTGTTAGGCAATATAAGCAGGCAGATTGTGAACTGGTAAAGATTGACATCAATTGCCATATTCTCGGTGATGTCGTGCTAGAGTGCATTACCCTAGGTAGTGATCTTGAGAGGGAAGAGATGATGTTTCGGGTCGTGTTCAATACTGCATTTCTTAGATCAAACATTCTAACACTTAACCGTGGTGAGATTGATGTACTGTGGAATACAACGGATCGGTTTCCCAAGGATTTCAGTGCAGAGGTTATATTTTCGGAGATGGGTGCTGGTAAAAAGCTTGCCTCTGTTGATCTGCCACATATGGAGGAAAAAGATGTTTTACCTATGGAAGCATTTGCTAAGGTTCAAGAGATATTTAGTGAAGCTGAATGGTTGGATCCAAATTCTGATGTTGCTGTGACTGTTTTCAACCAAATAACAGCAGCAAATATCCTTCAAGAAAGCTTGGATTCAGGTTCTCCTCGAAGCCCTGACTCACGTAGCCTATTGGAATCGGCACTTGAGAAAGTTAAGGAGAAAACCAAGTTGATGATATCGGAAAATATTGTTTCAAGTCCTGATACATCCTCTCCTGAAAAAGAGAAAGATACAATGTCCTCTCATAAATCTTATGCAGATCCGAATTCAATCCTTAAGAAGGTTGACGAATCGCGTGGACTTCGAGTTTCAGTCCAAAGAAATGTTCACTCAAAGATATTTTCACCTCGAATGGTCCAAAGTCCAGTAACATCACCACTTCCAAACCGTAGCCCCACACAAGGATCACCTGCTTCAATATCCAGATTTCACAGCTCACCCTCTAGCCTTGGGATCACTTCAATATTACATGATCATGGTTCATGTAAAGATGAAGAGTCTACTTCATCATCACCAGCTTCACCGTCAATTTCGTTTCTACCAACATTGCATCCGCTGACATCATCGCAACCCAAAAAAGCTTCTCCACAATGTCCACAGTCTCCCACACCTGTTCATTCAAATGGCCCTCCTTCAGCAGAAGCAGCAGTGACGTCATCACCATTGCCACCACTGAAGCCACTGAGGATTTTGTCTCGTCCGCCTCCACCCCCACCCCCACCTCCCATATCCTCTCTGCGCTCCACTCCTTCACCAAGTTCCACCAGCAATTCCATAGCAACACAAGGGCCTCCTCCACCTCCACCTCCGCCTCCTCTACAATCTCACCGTTCTGCACTTTCATCTTCTCCTCTGCCACCACCTTTACCTCCAAAGAAATTGTTAGCTACAACTAATCCTCCACCTCCACCACCACCACCTTTGCATTCTAATTCTCGAATGGGAGCCCCCACAAGTTCTTTGGTGTTGAAATCTCCTCCTGTTCCTCCTCCACCTGCCCCTGCACCATTGTCTCGTTCTCATAATGGAAATATTCCTCCAGTCCCTGGACCACCATTGGGTTTAAAGGGGCGTGGGATTTTACAGAATCTTAAAGGTCAGGGTCAAACGAGAAAGGCCAACTTGAAACCGTATCATTGGTTAAAGCTTACTAGGGCAGTGCAAGGAAGTTTATGGGCTGAAGCGCAGAAATCTGATGAAGCTGCAACTGCTCCAGATTTTGACATCTCTGAGCTTGAGAAACTCTTCTCAGCAGTGAATCTTAGCTCTGACAGTGAAAACAATGGTGGGAAATCAGGTCGACGTGCCCGACCTAAAGTTGAGAAAGTGCAGCTGATTGAGCTCAGACGAGCATACAACTGTGAGATCATGCTCTCTAAAGTTAAAATACCTTTGCCTGATTTGATGAGTTCTGTTCTTGCTTTGGATGAGTCAGTGATAGATGTTGATCAAGTTGACAATCTAATAAAGTTTTGTCCAACAAAAGAAGAAGCAGAATTGCTCAAGGGCTTCACTGGAAATAAAGAAACTTTGGGGCGGTGTGAGCAGTTCTTTTTAGAACTGCTGAAAGTTCCTCGAGTGGAGACTAAGCTAAGGGTTTTCTCATTCAAGATTCAGTTTCACTCCCAAGTTACTGATCTTAGAAGAGGTTTGAATACCATACATAGTGCAGCCAATGAGGTTAGAGGCTCGGCTAAACTAAAAAGAATCATGCAAACTATACTTTCCTTGGGCAATGCATTGAACCACGGGACTGCAAGGGAGACATTGGTTCTATTCAAGAATTTGAATTCGCTTCTGCATTTTTTTTTATATATTTCTTCTCTTTTGACAGGCTCAGCTATCGGATTTCGCCTGGATAGTCTTCTGAAACTCACTGACACTAGATCCCGTAACAGTAAGATGACTCTGATGCACTATCTCTGTAAGGTGCTTGCCGAGAAACTTCCAGAGCTACTTAATTTTCCAAAAGACTTGGTGAGCTTGGAGGCTGCGACAAAGATTCAACTAAAATACCTGGCAGAGGAGATGCAAGCAATTAGCAAAGGGTTGGAGAAAGTTGTACAAGAGTTTACTGCATCTGAAACTGATGGTCAAATATCAAAACACTTTCGAATGAACTTGAAAGAGTTTCTTAGCGTTGCTGAAGGAGAAGTAAGGTCCTTGGCTTCTCTCTATTCGACTGTGGGTGGAAGCGCCGATGCTTTGGCGTTATACTTCGGAGAAGATCCAGCTCGTGTCCCATTTGAGCAAGTTGTATCCACTCTGCAAAACTTTGTTAGAATATTTGTGCGATCACACGAGGAAAACTGCAAGCAAGTTGAGTTTGAGAAGAAGAGAGCACAGAAGGAAGCAGAGAATGAAAAACTTAAGAAAGGTGTGTACAATGAGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0030029 GO:0030036 GO:0032501 GO:0032502 GO:0034293 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044877 GO:0048229 GO:0048236 GO:0048856 GO:0048869 GO:0051015 GO:0051017 GO:0051321 GO:0051704 GO:0061572 GO:0071840 GO:0097435 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FH2 PTEN_C2

Protein Analysis

1168

Amino Acids

129.98

Weight (kDa)

8.6

Isoelectric Point (pI)

57.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PTEN_C2	PF10409	199 - 337	6.8e-37	C2 domain of PTEN tumour-suppressor protein
FH2	PF02181	740 - 969	8e-60	Formin Homology 2 Domain
FH2	PF02181	965 - 1133	6.5e-43	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003178)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25050 AT3G32400 AT3G32400 AT3G32410
fragaria_vesca	FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080
malus_domestica	MD00G1143700.v1.1 MD15G1039700.v1.1
prunus_persica	Prupe.1G393200_v2.0.a1
pyrus_communis	pycom08g04710 pycom15g03710
rosa_chinensis	RchiOBHm_Chr6g0299461
rosa_laevigata	RLG00000011429
rosa_multiflora	Rmu_co8403397.1_g000001 Rmu_sc0001184.1_g000009
rosa_roxburghii	Rroxscaffold_7G00168650
rosa_rugosa	Rorug06G0288500 Rorug06G0288600 Rorug06G0288700 Rorug06G0288800 Rorug06G0288900 Rorug06G0288900
rosa_samantha	Rh6AG400300 Rh6BG409000 Rh6CG414400 Rh6DG401400
rosa_wichuraiana	Rw6G035040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1528, 2113
AatII	GACGTC	1 cut(s)	1766
Acc36I	ACCTGC	2 cut(s)	1528, 2113
AccB7I	CCANNNNNTGG	1 cut(s)	2156
AccI	GTMKAC	2 cut(s)	1610, 2410
AccII	CGCG	1 cut(s)	1405
AciI	CCGC	9 cut(s)	136, 387, 433, 524, 590, 1664, 1811, 1919, 2647
AclWI	GGATC	9 cut(s)	686, 975, 1124, 1137, 1364, 1522, 1571, 2991, 3332
AcoI	YGGCCR	2 cut(s)	392, 584
AcsI	RAATTY	4 cut(s)	1135, 1375, 2893, 2899
AcuI	CTGAAG	6 cut(s)	60, 533, 1728, 1805, 2310, 3276
AcyI	GRCGYC	1 cut(s)	1763
AfaI	GTAC	4 cut(s)	231, 951, 3174, 3494
AfiI	CCNNNNNNNGG	6 cut(s)	41, 1390, 1511, 2156, 2168, 3133
AflII	CTTAAG	2 cut(s)	1385, 3479
AjiI	CACGTC	1 cut(s)	2414
AjnI	CCWGG	4 cut(s)	597, 2155, 2964, 3126
AjuI	GAANNNNNNNTTGG	2 cut(s)	1419, 1451
Alw21I	GWGCWC	2 cut(s)	2454, 3454
Alw26I	GTCTC	6 cut(s)	516, 1716, 1809, 2127, 2687, 2867
AlwI	GGATC	9 cut(s)	686, 975, 1124, 1137, 1364, 1522, 1571, 2991, 3332
AlwNI	CAGNNNCTG	1 cut(s)	2156
Ama87I	CYCGRG	2 cut(s)	559, 2685
AoxI	GGCC	6 cut(s)	392, 584, 600, 1735, 1898, 2226
ApeKI	GCWGC	8 cut(s)	84, 526, 1175, 1754, 2312, 2440, 2780, 3101
ApoI	RAATTY	4 cut(s)	1135, 1375, 2893, 2899
Asp700I	GAANNNNTTC	2 cut(s)	162, 2354
AspA2I	CCTAGG	1 cut(s)	847
AspLEI	GCGC	3 cut(s)	1851, 2296, 3308
AspS9I	GGNCC	6 cut(s)	601, 1465, 1736, 1898, 2159, 3270
AsuC2I	CCSGG	2 cut(s)	168, 197
AsuII	TTCGAA	1 cut(s)	3223
AvaI	CYCGRG	2 cut(s)	559, 2685
AvaII	GGWCC	3 cut(s)	1465, 2159, 3270
AvrII	CCTAGG	1 cut(s)	847
BaeGI	GKGCMC	1 cut(s)	2419
BalI	TGGCCA	1 cut(s)	586
BamHI	GGATCC	1 cut(s)	1129
BanII	GRGCYC	2 cut(s)	2059, 2454
BauI	CACGAG	3 cut(s)	489, 3345, 3410
BbsI	GAAGAC	1 cut(s)	2966
Bbv12I	GWGCWC	2 cut(s)	2454, 3454
BbvI	GCAGC	8 cut(s)	71, 513, 1187, 1766, 2299, 2452, 2792, 3088
BccI	CCATC	7 cut(s)	194, 214, 260, 384, 641, 1006, 3194
BcgI	CGANNNNNNTGC	2 cut(s)	470, 504
BciT130I	CCWGG	4 cut(s)	599, 2157, 2966, 3128
BciVI	GTATCC	1 cut(s)	3379
BclI	TGATCA	2 cut(s)	1582, 2557
BcnI	CCSGG	2 cut(s)	168, 197
BcoDI	GTCTC	6 cut(s)	516, 1716, 1809, 2127, 2687, 2867
BfaI	CTAG	7 cut(s)	464, 593, 833, 848, 1554, 2262, 2994
BfoI	RGCGCY	1 cut(s)	3309
BfrI	CTTAAG	2 cut(s)	1385, 3479
BfuAI	ACCTGC	2 cut(s)	1528, 2113
BfuI	GTATCC	1 cut(s)	3379
BglII	AGATCT	1 cut(s)	54
BisI	GCNGC	9 cut(s)	85, 136, 527, 1176, 1755, 2313, 2441, 2781, 3102
BlnI	CCTAGG	1 cut(s)	847
BlpI	GCTNAGC	1 cut(s)	2947
BlsI	GCNGC	9 cut(s)	86, 137, 528, 1177, 1756, 2314, 2442, 2782, 3103
Bme1390I	CCNGG	6 cut(s)	168, 197, 599, 2157, 2966, 3128
Bme18I	GGWCC	3 cut(s)	1465, 2159, 3270
BmeT110I	CYCGRG	2 cut(s)	559, 2685
BmgBI	CACGTC	1 cut(s)	2414
BmgT120I	GGNCC	6 cut(s)	601, 1465, 1736, 1898, 2159, 3270
BmiI	GGNNCC	3 cut(s)	486, 1131, 2056
BmrFI	CCNGG	6 cut(s)	168, 197, 599, 2157, 2966, 3128
BmsI	GCATC	8 cut(s)	182, 319, 726, 1669, 3012, 3129, 3197, 3301
BpiI	GAAGAC	1 cut(s)	2966
BpmI	CTGGAG	2 cut(s)	2133, 2307
Bpu10I	CCTNAGC	2 cut(s)	1092, 2702
Bpu1102I	GCTNAGC	1 cut(s)	2947
Bpu14I	TTCGAA	1 cut(s)	3223
BpuEI	CTTGAG	5 cut(s)	71, 881, 1274, 2366, 2597
BpuMI	CCSGG	2 cut(s)	168, 197
BsaAI	YACGTR	1 cut(s)	1232
BsaBI	GATNNNNATC	2 cut(s)	800, 2556
BsaHI	GRCGYC	1 cut(s)	1763
BsaJI	CCNNGG	9 cut(s)	93, 847, 934, 978, 1558, 2155, 2838, 2857, 3273
BsaXI	ACNNNNNCTCC	4 cut(s)	1306, 1336, 1993, 2023
Bsc4I	CCNNNNNNNGG	6 cut(s)	41, 1390, 1511, 2156, 2168, 3133
Bse1I	ACTGG	4 cut(s)	792, 1475, 2150, 2629
Bse3DI	GCAATG	3 cut(s)	1655, 1772, 2851
Bse8I	GATNNNNATC	2 cut(s)	800, 2556
BseBI	CCWGG	4 cut(s)	599, 2157, 2966, 3128
BseDI	CCNNGG	9 cut(s)	93, 847, 934, 978, 1558, 2155, 2838, 2857, 3273
BseGI	GGATG	6 cut(s)	205, 225, 395, 1316, 1660, 2542
BseJI	GATNNNNATC	2 cut(s)	800, 2556
BseLI	CCNNNNNNNGG	6 cut(s)	41, 1390, 1511, 2156, 2168, 3133
BseMI	GCAATG	3 cut(s)	1655, 1772, 2851
BseMII	CTCAG	7 cut(s)	72, 228, 1784, 2331, 2373, 2467, 2961
BseNI	ACTGG	4 cut(s)	792, 1475, 2150, 2629
BseRI	GAGGAG	9 cut(s)	477, 577, 1203, 1893, 1914, 1950, 2076, 2088, 3149
BseSI	GKGCMC	1 cut(s)	2419
BseXI	GCAGC	8 cut(s)	71, 513, 1187, 1766, 2299, 2452, 2792, 3088
BsgI	GTGCAG	5 cut(s)	1011, 1929, 2097, 2459, 2799
Bsh1236I	CGCG	1 cut(s)	1405
BshFI	GGCC	6 cut(s)	394, 586, 602, 1737, 1900, 2228
BsiHKAI	GWGCWC	2 cut(s)	2454, 3454
BsiHKCI	CYCGRG	2 cut(s)	559, 2685
BsiSI	CCGG	3 cut(s)	168, 196, 217
BslFI	GGGAC	3 cut(s)	2138, 2875, 3335
BslI	CCNNNNNNNGG	6 cut(s)	41, 1390, 1511, 2156, 2168, 3133
BsmAI	GTCTC	6 cut(s)	516, 1716, 1809, 2127, 2687, 2867
BsmFI	GGGAC	3 cut(s)	2138, 2875, 3335
BsmI	GAATGC	1 cut(s)	2035
BsnI	GGCC	6 cut(s)	394, 586, 602, 1737, 1900, 2228
BsoBI	CYCGRG	2 cut(s)	559, 2685
Bsp119I	TTCGAA	1 cut(s)	3223
Bsp1286I	GDGCHC	4 cut(s)	2059, 2419, 2454, 3454
Bsp1407I	TGTACA	2 cut(s)	3172, 3492
Bsp1720I	GCTNAGC	1 cut(s)	2947
BspACI	CCGC	9 cut(s)	136, 387, 433, 524, 590, 1664, 1811, 1919, 2647
BspANI	GGCC	6 cut(s)	394, 586, 602, 1737, 1900, 2228
BspCNI	CTCAG	7 cut(s)	71, 229, 1785, 2332, 2372, 2466, 2960
BspFNI	CGCG	1 cut(s)	1405
BspLI	GGNNCC	3 cut(s)	486, 1131, 2056
BspMI	ACCTGC	2 cut(s)	1528, 2113
BspPI	GGATC	9 cut(s)	686, 975, 1124, 1137, 1364, 1522, 1571, 2991, 3332
BspT104I	TTCGAA	1 cut(s)	3223
BspTI	CTTAAG	2 cut(s)	1385, 3479
BsrDI	GCAATG	3 cut(s)	1655, 1772, 2851
BsrGI	TGTACA	2 cut(s)	3172, 3492
BsrI	ACTGG	4 cut(s)	792, 1475, 2150, 2629
BssECI	CCNNGG	9 cut(s)	93, 847, 934, 978, 1558, 2155, 2838, 2857, 3273
BssNI	GRCGYC	1 cut(s)	1763
BssSI	CACGAG	3 cut(s)	489, 3345, 3410
BssT1I	CCWWGG	5 cut(s)	847, 978, 1558, 2838, 3273
Bst2BI	CACGAG	3 cut(s)	489, 3345, 3410
Bst2UI	CCWGG	4 cut(s)	599, 2157, 2966, 3128
Bst6I	CTCTTC	5 cut(s)	864, 1599, 2360, 2749, 3440
BstACI	GRCGYC	1 cut(s)	1763
BstAFI	CTTAAG	2 cut(s)	1385, 3479
BstAUI	TGTACA	2 cut(s)	3172, 3492
BstBAI	YACGTR	1 cut(s)	1232
BstBI	TTCGAA	1 cut(s)	3223
BstC8I	GCNNGC	7 cut(s)	414, 483, 774, 1032, 3045, 3144, 3425
BstDSI	CCRYGG	3 cut(s)	93, 934, 2857
BstENI	CCTNNNNNAGG	3 cut(s)	39, 1388, 3131
BstF5I	GGATG	6 cut(s)	205, 225, 395, 1316, 1660, 2542
BstFNI	CGCG	1 cut(s)	1405
BstH2I	RGCGCY	1 cut(s)	3309
BstHHI	GCGC	3 cut(s)	1851, 2296, 3308
BstMAI	GTCTC	6 cut(s)	516, 1716, 1809, 2127, 2687, 2867
BstMWI	GCNNNNNNNGC	7 cut(s)	379, 418, 769, 1754, 2318, 2449, 2653
BstNI	CCWGG	4 cut(s)	599, 2157, 2966, 3128
BstSCI	CCNGG	6 cut(s)	166, 195, 597, 2155, 2964, 3126
BstSLI	GKGCMC	1 cut(s)	2419
BstUI	CGCG	1 cut(s)	1405
BstV1I	GCAGC	8 cut(s)	71, 513, 1187, 1766, 2299, 2452, 2792, 3088
BstV2I	GAAGAC	1 cut(s)	2966
BstX2I	RGATCY	6 cut(s)	54, 691, 1129, 1369, 2996, 3337
BstYI	RGATCY	6 cut(s)	54, 691, 1129, 1369, 2996, 3337
BsuI	GTATCC	1 cut(s)	3379
BsuRI	GGCC	6 cut(s)	394, 586, 602, 1737, 1900, 2228
BtgI	CCRYGG	3 cut(s)	93, 934, 2857
BtgZI	GCGATG	1 cut(s)	1659
BtrI	CACGTC	1 cut(s)	2414
BtsCI	GGATG	6 cut(s)	205, 225, 395, 1316, 1660, 2542
BtsI	GCAGTG	3 cut(s)	1764, 2274, 2370
BveI	ACCTGC	2 cut(s)	1528, 2113
Cac8I	GCNNGC	7 cut(s)	414, 483, 774, 1032, 3045, 3144, 3425
CaiI	CAGNNNCTG	1 cut(s)	2156
CfoI	GCGC	3 cut(s)	1851, 2296, 3308
Cfr13I	GGNCC	6 cut(s)	601, 1465, 1736, 1898, 2159, 3270
Csp6I	GTAC	4 cut(s)	230, 950, 3173, 3493
CspCI	CAANNNNNGTGG	2 cut(s)	2013, 2048
CviAII	CATG	8 cut(s)	181, 247, 256, 1580, 1586, 1593, 2479, 2821
CviQI	GTAC	4 cut(s)	230, 950, 3173, 3493
DraI	TTTAAA	1 cut(s)	2175
EaeI	YGGCCR	2 cut(s)	392, 584
Eam1104I	CTCTTC	5 cut(s)	864, 1599, 2360, 2749, 3440
EarI	CTCTTC	5 cut(s)	864, 1599, 2360, 2749, 3440
EciI	GGCGGA	3 cut(s)	402, 1800, 1908
Ecl136II	GAGCTC	1 cut(s)	2452
Eco130I	CCWWGG	5 cut(s)	847, 978, 1558, 2838, 3273
Eco24I	GRGCYC	2 cut(s)	2059, 2454
Eco32I	GATATC	1 cut(s)	1287
Eco47I	GGWCC	3 cut(s)	1465, 2159, 3270
Eco53kI	GAGCTC	1 cut(s)	2452
Eco57I	CTGAAG	6 cut(s)	60, 533, 1728, 1805, 2310, 3276
Eco88I	CYCGRG	2 cut(s)	559, 2685
EcoICRI	GAGCTC	1 cut(s)	2452
EcoNI	CCTNNNNNAGG	3 cut(s)	39, 1388, 3131
EcoO109I	RGGNCCY	3 cut(s)	601, 1898, 3270
EcoRI	GAATTC	2 cut(s)	1375, 2899
EcoRII	CCWGG	4 cut(s)	597, 2155, 2964, 3126
EcoRV	GATATC	1 cut(s)	1287
EcoT14I	CCWWGG	5 cut(s)	847, 978, 1558, 2838, 3273
EcoT22I	ATGCAT	1 cut(s)	2851
EcoT38I	GRGCYC	2 cut(s)	2059, 2454
ErhI	CCWWGG	5 cut(s)	847, 978, 1558, 2838, 3273
FaeI	CATG	8 cut(s)	184, 250, 259, 1583, 1589, 1596, 2482, 2824
FalI	AAGNNNNNCTT	6 cut(s)	33, 65, 2216, 2248, 3228, 3260
FaqI	GGGAC	3 cut(s)	2138, 2875, 3335
FatI	CATG	8 cut(s)	180, 246, 255, 1579, 1585, 1592, 2478, 2820
FauNDI	CATATG	1 cut(s)	1053
FbaI	TGATCA	2 cut(s)	1582, 2557
FblI	GTMKAC	2 cut(s)	1610, 2410
Fnu4HI	GCNGC	9 cut(s)	85, 136, 527, 1176, 1755, 2313, 2441, 2781, 3102
FokI	GGATG	6 cut(s)	212, 232, 402, 1303, 1647, 2549
FriOI	GRGCYC	2 cut(s)	2059, 2454
Fsp4HI	GCNGC	9 cut(s)	85, 136, 527, 1176, 1755, 2313, 2441, 2781, 3102
FspBI	CTAG	7 cut(s)	464, 593, 833, 848, 1554, 2262, 2994
GlaI	GCGC	3 cut(s)	1850, 2295, 3307
GluI	GCNGC	9 cut(s)	85, 136, 527, 1176, 1755, 2313, 2441, 2781, 3102
GsuI	CTGGAG	2 cut(s)	2133, 2307
HaeII	RGCGCY	1 cut(s)	3309
HaeIII	GGCC	6 cut(s)	394, 586, 602, 1737, 1900, 2228
HapII	CCGG	3 cut(s)	168, 196, 217
HhaI	GCGC	3 cut(s)	1851, 2296, 3308
Hin1I	GRCGYC	1 cut(s)	1763
Hin1II	CATG	8 cut(s)	184, 250, 259, 1583, 1589, 1596, 2482, 2824
Hin6I	GCGC	3 cut(s)	1849, 2294, 3306
HinP1I	GCGC	3 cut(s)	1849, 2294, 3306
HincII	GTYRAC	4 cut(s)	353, 1396, 2411, 2566
HindII	GTYRAC	4 cut(s)	353, 1396, 2411, 2566
HindIII	AAGCTT	4 cut(s)	1028, 1195, 1689, 2255
HpaII	CCGG	3 cut(s)	168, 196, 217
Hpy99I	CGWCG	1 cut(s)	2415
HpyAV	CCTTC	9 cut(s)	35, 272, 540, 1196, 1384, 1752, 1869, 3251, 3451
HpyCH4IV	ACGT	4 cut(s)	72, 1231, 1763, 2413
HpyF10VI	GCNNNNNNNGC	7 cut(s)	379, 418, 769, 1754, 2318, 2449, 2653
HpySE526I	ACGT	4 cut(s)	72, 1231, 1763, 2413
Hsp92I	GRCGYC	1 cut(s)	1763
Hsp92II	CATG	8 cut(s)	184, 250, 259, 1583, 1589, 1596, 2482, 2824
HspAI	GCGC	3 cut(s)	1849, 2294, 3306
Ksp22I	TGATCA	2 cut(s)	1582, 2557
LmnI	GCTCC	4 cut(s)	490, 1856, 2054, 2326
Lsp1109I	GCAGC	8 cut(s)	71, 513, 1187, 1766, 2299, 2452, 2792, 3088
LweI	GCATC	8 cut(s)	182, 319, 726, 1669, 3012, 3129, 3197, 3301
MaeI	CTAG	7 cut(s)	464, 593, 833, 848, 1554, 2262, 2994
MaeII	ACGT	4 cut(s)	72, 1231, 1763, 2413
MaeIII	GTNAC	6 cut(s)	498, 1150, 1477, 1759, 2740, 3002
MfeI	CAATTG	1 cut(s)	805
MflI	RGATCY	6 cut(s)	54, 691, 1129, 1369, 2996, 3337
MhlI	GDGCHC	4 cut(s)	2059, 2419, 2454, 3454
MlsI	TGGCCA	1 cut(s)	586
MluNI	TGGCCA	1 cut(s)	586
MlyI	GAGTC	5 cut(s)	154, 1220, 1616, 2549, 3010
MmeI	TCCRAC	4 cut(s)	84, 1107, 2612, 3141
Mox20I	TGGCCA	1 cut(s)	586
Mph1103I	ATGCAT	1 cut(s)	2851
MroXI	GAANNNNTTC	2 cut(s)	162, 2354
MscI	TGGCCA	1 cut(s)	586
MslI	CAYNNNNRTG	3 cut(s)	98, 1584, 1731
Msp20I	TGGCCA	1 cut(s)	586
MspA1I	CMGCKG	4 cut(s)	329, 526, 1666, 2443
MspCI	CTTAAG	2 cut(s)	1385, 3479
MspI	CCGG	3 cut(s)	168, 196, 217
MspR9I	CCNGG	6 cut(s)	168, 197, 599, 2157, 2966, 3128
MunI	CAATTG	1 cut(s)	805
Mva1269I	GAATGC	1 cut(s)	2035
MvaI	CCWGG	4 cut(s)	599, 2157, 2966, 3128
MvnI	CGCG	1 cut(s)	1405
MwoI	GCNNNNNNNGC	7 cut(s)	379, 418, 769, 1754, 2318, 2449, 2653
NciI	CCSGG	2 cut(s)	168, 197
NdeI	CATATG	1 cut(s)	1053
NlaIII	CATG	8 cut(s)	184, 250, 259, 1583, 1589, 1596, 2482, 2824
NlaIV	GGNNCC	3 cut(s)	486, 1131, 2056
NmeAIII	GCCGAG	2 cut(s)	2780, 3073
NmuCI	GTSAC	2 cut(s)	1150, 1759
NsiI	ATGCAT	1 cut(s)	2851
NspV	TTCGAA	1 cut(s)	3223
PaeR7I	CTCGAG	2 cut(s)	559, 2685
PaqCI	CACCTGC	2 cut(s)	1528, 2113
PcsI	WCGNNNNNNNCGW	2 cut(s)	825, 2416
PctI	GAATGC	1 cut(s)	2035
PdmI	GAANNNNTTC	2 cut(s)	162, 2354
PfeI	GAWTC	9 cut(s)	634, 1202, 1244, 1400, 2197, 2368, 2722, 2817, 3112
PflMI	CCANNNNNTGG	1 cut(s)	2156
PfoI	TCCNGGA	1 cut(s)	195
PkrI	GCNGC	9 cut(s)	86, 137, 528, 1177, 1756, 2314, 2442, 2782, 3103
PleI	GAGTC	5 cut(s)	153, 1220, 1615, 2548, 3010
PpsI	GAGTC	5 cut(s)	153, 1220, 1615, 2548, 3010
Ppu21I	YACGTR	1 cut(s)	1232
PpuMI	RGGWCCY	1 cut(s)	3270
Psp124BI	GAGCTC	1 cut(s)	2454
Psp5II	RGGWCCY	1 cut(s)	3270
Psp6I	CCWGG	4 cut(s)	597, 2155, 2964, 3126
PspGI	CCWGG	4 cut(s)	597, 2155, 2964, 3126
PspN4I	GGNNCC	3 cut(s)	486, 1131, 2056
PspPI	GGNCC	6 cut(s)	601, 1465, 1736, 1898, 2159, 3270
PspPPI	RGGWCCY	1 cut(s)	3270
PspXI	VCTCGAGB	1 cut(s)	2685
PstNI	CAGNNNCTG	1 cut(s)	2156
PsuI	RGATCY	6 cut(s)	54, 691, 1129, 1369, 2996, 3337
PvuII	CAGCTG	2 cut(s)	329, 2443
RsaI	GTAC	4 cut(s)	231, 951, 3174, 3494
RsaNI	GTAC	4 cut(s)	230, 950, 3173, 3493
RseI	CAYNNNNRTG	3 cut(s)	98, 1584, 1731
SacI	GAGCTC	1 cut(s)	2454
SalI	GTCGAC	1 cut(s)	2409
SatI	GCNGC	9 cut(s)	85, 136, 527, 1176, 1755, 2313, 2441, 2781, 3102
Sau96I	GGNCC	6 cut(s)	601, 1465, 1736, 1898, 2159, 3270
SchI	GAGTC	5 cut(s)	154, 1220, 1616, 2549, 3010
ScrFI	CCNGG	6 cut(s)	168, 197, 599, 2157, 2966, 3128
SduI	GDGCHC	4 cut(s)	2059, 2419, 2454, 3454
SfaNI	GCATC	8 cut(s)	182, 319, 726, 1669, 3012, 3129, 3197, 3301
Sfr274I	CTCGAG	2 cut(s)	559, 2685
SfuI	TTCGAA	1 cut(s)	3223
SinI	GGWCC	3 cut(s)	1465, 2159, 3270
SlaI	CTCGAG	2 cut(s)	559, 2685
SmiMI	CAYNNNNRTG	3 cut(s)	98, 1584, 1731
SmlI	CTYRAG	9 cut(s)	50, 559, 860, 1253, 1385, 2345, 2612, 2685, 3479
SmoI	CTYRAG	9 cut(s)	50, 559, 860, 1253, 1385, 2345, 2612, 2685, 3479
SsiI	CCGC	9 cut(s)	136, 387, 433, 524, 590, 1664, 1811, 1919, 2647
SspI	AATATT	4 cut(s)	1297, 1575, 2143, 3396
SspMI	CTAG	7 cut(s)	464, 593, 833, 848, 1554, 2262, 2994
SstI	GAGCTC	1 cut(s)	2454
StyD4I	CCNGG	6 cut(s)	166, 195, 597, 2155, 2964, 3126
StyI	CCWWGG	5 cut(s)	847, 978, 1558, 2838, 3273
TaiI	ACGT	4 cut(s)	75, 1234, 1766, 2416
TatI	WGTACW	4 cut(s)	229, 949, 3172, 3492
TauI	GCSGC	1 cut(s)	138
TfiI	GAWTC	9 cut(s)	634, 1202, 1244, 1400, 2197, 2368, 2722, 2817, 3112
TseFI	GTSAC	2 cut(s)	1150, 1759
TseI	GCWGC	8 cut(s)	84, 526, 1175, 1754, 2312, 2440, 2780, 3101
Tsp45I	GTSAC	2 cut(s)	1150, 1759
TspGWI	ACGGA	2 cut(s)	110, 980
Van91I	CCANNNNNTGG	1 cut(s)	2156
Vha464I	CTTAAG	2 cut(s)	1385, 3479
VpaK11BI	GGWCC	3 cut(s)	1465, 2159, 3270
XagI	CCTNNNNNAGG	3 cut(s)	39, 1388, 3131
XapI	RAATTY	4 cut(s)	1135, 1375, 2893, 2899
XcmI	CCANNNNNNNNNTGG	2 cut(s)	100, 2068
XhoI	CTCGAG	2 cut(s)	559, 2685
XmaJI	CCTAGG	1 cut(s)	847
XmiI	GTMKAC	2 cut(s)	1610, 2410
XmnI	GAANNNNTTC	2 cut(s)	162, 2354
XspI	CTAG	7 cut(s)	464, 593, 833, 848, 1554, 2262, 2994
ZraI	GACGTC	1 cut(s)	1764
Zsp2I	ATGCAT	1 cut(s)	2851

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.