Rorug06G0288700

C2 domain of PTEN tumour-suppressor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003178
Physical Location & Seq
Reverse (-)
43878643 .. 43879140
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0288700.1

Sequence Viewer

Length: 498 bp
ATGGCCGACCCGGAAACCGACGAGGTCTTCGCCAGAATCTGGCTTCTCCCTTCCGAGAGCAGCGAGCTTTTTGCTCAAGACCGGGGCTATGTGGACTCTGATGGGTCTGACAATCTGGAAAAGCCTTCTTCTTTAGCTAAAACTCTGACCCAGTCCGACGCCAACAATGGCGGCAGCTTCTCCGTCCCGAGGTACTGCGTTGAGATCATTTTCTTGAGGCTCGACTACTCCGTTGACCCGCCTGTGCAGACGGTGGTCGCCAAGGACGTCCACGGCGAGGTGTGGAAGTTTCAGCACATTTACCTAGGCATCCCTCGCAGGCATTTGCTGATGACTGAGTGGAGCATGTTCGTGAACCAGAAGAAGCTCGTTGTCGGTGACTCGATTGTGTTCCTGCGAATCAAAAATGGCGACCTCTGCGTCTGGATTTGGCGGGCCAAGCGGGGATCGCCGGATGGGTGGTCGGAATCAGGGTGGAACCACGGTGGTTCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FH2 PTEN_C2

Protein Analysis

165

Amino Acids

18.7

Weight (kDa)

5.54

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 46 - 145 1.1e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 419
AatII GACGTC 1 cut(s) 270
AccB7I CCANNNNNTGG 1 cut(s) 39
AciI CCGC 4 cut(s) 171, 239, 433, 442
AclWI GGATC 1 cut(s) 454
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 2 cut(s) 159, 267
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 3 cut(s) 39, 189, 277
AluBI AGCT 4 cut(s) 67, 137, 177, 367
AluI AGCT 4 cut(s) 67, 137, 177, 367
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 39
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 2 cut(s) 3, 435
ApeKI GCWGC 2 cut(s) 60, 174
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspA2I CCTAGG 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 435
AsuC2I CCSGG 2 cut(s) 11, 83
AsuHPI GGTGA 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 187
AvrII CCTAGG 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 72, 186
BccI CCATC 2 cut(s) 95, 449
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BcnI CCSGG 2 cut(s) 11, 83
BfaI CTAG 2 cut(s) 305, 492
BisI GCNGC 3 cut(s) 61, 172, 175
BlnI CCTAGG 1 cut(s) 304
BlsI GCNGC 3 cut(s) 62, 173, 176
Bme1390I CCNGG 2 cut(s) 11, 83
BmeT110I CYCGRG 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 1 cut(s) 479
BmrFI CCNGG 2 cut(s) 11, 83
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 1 cut(s) 318
BmuI ACTGGG 1 cut(s) 145
BoxI GACNNNNGTC 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 19
BpuEI CTTGAG 2 cut(s) 60, 235
BpuMI CCSGG 2 cut(s) 11, 83
BsaHI GRCGYC 2 cut(s) 159, 267
BsaJI CCNNGG 6 cut(s) 82, 188, 261, 271, 304, 481
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 189, 277
Bse1I ACTGG 1 cut(s) 151
BseDI CCNNGG 6 cut(s) 82, 188, 261, 271, 304, 481
BseGI GGATG 2 cut(s) 309, 460
BseLI CCNNNNNNNGG 3 cut(s) 39, 189, 277
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 72, 186
BsgI GTGCAG 1 cut(s) 266
BshFI GGCC 2 cut(s) 5, 437
BsiHKCI CYCGRG 1 cut(s) 187
BsiSI CCGG 3 cut(s) 11, 82, 452
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 39, 189, 277
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 2 cut(s) 5, 437
BsoBI CYCGRG 1 cut(s) 187
Bsp143I GATC 2 cut(s) 204, 446
BspACI CCGC 4 cut(s) 171, 239, 433, 442
BspANI GGCC 2 cut(s) 5, 437
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 479
BspPI GGATC 1 cut(s) 454
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 6 cut(s) 82, 188, 261, 271, 304, 481
BssMI GATC 2 cut(s) 204, 446
BssNI GRCGYC 2 cut(s) 159, 267
BssT1I CCWWGG 2 cut(s) 261, 304
Bst4CI ACNGT 2 cut(s) 253, 485
BstACI GRCGYC 2 cut(s) 159, 267
BstC8I GCNNGC 3 cut(s) 65, 320, 435
BstDEI CTNAG 1 cut(s) 336
BstDSI CCRYGG 2 cut(s) 271, 481
BstF5I GGATG 2 cut(s) 309, 460
BstKTI GATC 2 cut(s) 207, 449
BstMBI GATC 2 cut(s) 204, 446
BstMWI GCNNNNNNNGC 4 cut(s) 315, 417, 439, 448
BstNSI RCATGY 1 cut(s) 349
BstPAI GACNNNNGTC 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 9, 81
BstV1I GCAGC 2 cut(s) 72, 186
BstV2I GAAGAC 1 cut(s) 19
BsuRI GGCC 2 cut(s) 5, 437
BtgI CCRYGG 2 cut(s) 271, 481
BtsCI GGATG 2 cut(s) 309, 460
Cac8I GCNNGC 3 cut(s) 65, 320, 435
CaiI CAGNNNCTG 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 435
CseI GACGC 2 cut(s) 167, 409
Csp6I GTAC 1 cut(s) 193
CviAII CATG 1 cut(s) 346
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 336
DpnI GATC 2 cut(s) 206, 448
DpnII GATC 2 cut(s) 204, 446
DrdI GACNNNNNNGTC 1 cut(s) 419
DseDI GACNNNNNNGTC 1 cut(s) 419
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 261, 304
Eco88I CYCGRG 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 261, 304
ErhI CCWWGG 2 cut(s) 261, 304
FaeI CATG 1 cut(s) 349
FaiI YATR 2 cut(s) 90, 347
FaqI GGGAC 1 cut(s) 170
FatI CATG 1 cut(s) 345
FauI CCCGC 3 cut(s) 246, 426, 435
Fnu4HI GCNGC 3 cut(s) 61, 172, 175
FokI GGATG 2 cut(s) 296, 467
Fsp4HI GCNGC 3 cut(s) 61, 172, 175
FspBI CTAG 2 cut(s) 305, 492
GluI GCNGC 3 cut(s) 61, 172, 175
HaeIII GGCC 2 cut(s) 5, 437
HapII CCGG 3 cut(s) 11, 82, 452
HgaI GACGC 2 cut(s) 167, 409
Hin1I GRCGYC 2 cut(s) 159, 267
Hin1II CATG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 5 cut(s) 36, 95, 380, 399, 467
HpaII CCGG 3 cut(s) 11, 82, 452
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 4 cut(s) 94, 235, 271, 355
Hpy188I TCNGA 6 cut(s) 55, 100, 109, 147, 157, 466
Hpy188III TCNNGA 7 cut(s) 77, 116, 187, 214, 352, 424, 492
Hpy8I GTNNAC 4 cut(s) 94, 235, 271, 355
Hpy99I CGWCG 2 cut(s) 23, 161
HpyAV CCTTC 2 cut(s) 60, 135
HpyCH4III ACNGT 2 cut(s) 253, 485
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 1 cut(s) 247
HpyF10VI GCNNNNNNNGC 4 cut(s) 315, 417, 439, 448
HpyF3I CTNAG 1 cut(s) 336
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 2 cut(s) 159, 267
Hsp92II CATG 1 cut(s) 349
Kzo9I GATC 2 cut(s) 204, 446
LmnI GCTCC 1 cut(s) 342
Lsp1109I GCAGC 2 cut(s) 72, 186
LweI GCATC 1 cut(s) 318
MaeI CTAG 2 cut(s) 305, 492
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 1 cut(s) 377
MalI GATC 2 cut(s) 206, 448
MboI GATC 2 cut(s) 204, 446
MboII GAAGA 3 cut(s) 19, 120, 373
MlyI GAGTC 2 cut(s) 89, 374
MmeI TCCRAC 2 cut(s) 180, 444
MnlI CCTC 6 cut(s) 16, 183, 210, 271, 324, 425
MslI CAYNNNNRTG 1 cut(s) 350
MspI CCGG 3 cut(s) 11, 82, 452
MspR9I CCNGG 2 cut(s) 11, 83
MwoI GCNNNNNNNGC 4 cut(s) 315, 417, 439, 448
NciI CCSGG 2 cut(s) 11, 83
NdeII GATC 2 cut(s) 204, 446
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 1 cut(s) 479
NmuCI GTSAC 1 cut(s) 377
NspI RCATGY 1 cut(s) 349
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 264, 273
PfeI GAWTC 3 cut(s) 36, 399, 467
PflFI GACNNNGTC 2 cut(s) 23, 151
PflMI CCANNNNNTGG 1 cut(s) 39
PkrI GCNGC 3 cut(s) 62, 173, 176
PleI GAGTC 2 cut(s) 89, 374
PpsI GAGTC 2 cut(s) 89, 374
PshAI GACNNNNGTC 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 479
PspPI GGNCC 1 cut(s) 435
PstNI CAGNNNCTG 1 cut(s) 39
PsyI GACNNNGTC 2 cut(s) 23, 151
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 350
SatI GCNGC 3 cut(s) 61, 172, 175
Sau3AI GATC 2 cut(s) 204, 446
Sau96I GGNCC 1 cut(s) 435
SchI GAGTC 2 cut(s) 89, 374
ScrFI CCNGG 2 cut(s) 11, 83
SfaNI GCATC 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 75, 214
SmoI CTYRAG 2 cut(s) 75, 214
SsiI CCGC 4 cut(s) 171, 239, 433, 442
SspMI CTAG 2 cut(s) 305, 492
StyD4I CCNGG 2 cut(s) 9, 81
StyI CCWWGG 2 cut(s) 261, 304
TaaI ACNGT 2 cut(s) 253, 485
TaiI ACGT 1 cut(s) 270
TaqI TCGA 2 cut(s) 222, 383
TauI GCSGC 1 cut(s) 174
TfiI GAWTC 3 cut(s) 36, 399, 467
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 2 cut(s) 60, 174
Tsp45I GTSAC 1 cut(s) 377
TspGWI ACGGA 2 cut(s) 172, 220
Tth111I GACNNNGTC 2 cut(s) 23, 151
Van91I CCANNNNNTGG 1 cut(s) 39
XbaI TCTAGA 1 cut(s) 491
XceI RCATGY 1 cut(s) 349
XmaJI CCTAGG 1 cut(s) 304
XspI CTAG 2 cut(s) 305, 492
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.