Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003178

Physical Location & Seq

Genomic Coordinates Reverse (-)

13356995 .. 13360630

Gene Structure 3636 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G32400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGGAGCTGCTCAGTTCCATAGCAACAGAAGGGCCTCCTCCACCTCCACCTCCGCCTCTTCTACAACCTCACCATTCTGCACTTTCATCTTCTCCTCTGCCACCACCTTTACCTCCAAAGAAATTGTTAGCTACAACTAATACTCCACCTCCACCACCTCCACCTTTGCATTCTAATTCTCAAATGGGAGCCCCCACAAGTTCTTTGGTGTTGAAATCTCCTCATAATCTTAAAGGTCAGGGTCAAACGAGAAAGGCCAACTTGAAACCGTATCATTGGTTAAAGCTTACTAGAGCAGTGCAAGGAAGTTTATGGGCTGAAGCGCAGAAATCTGATGAAGCTGCAACTGCTCCAGATTTTGACATCTCTGAGATTGAGAAACTCTTCTCAGCAGTGAATCTTAGCTCTAACAGTGAAAATAATGGTGGGAAATCAGGCCGACGTGCCCGACCTAAAGTTGAGAAAGTGCAGCTGATTGAGCTCAAACGAGCATACAACTGTGAGATCATGCTCTCTAAAGTTAAAATACCTCTGCCTGATTTGATGAGTTCTGTTCTTGCTTTGGATGAGTCAGTGATAGATGTTGATCAAGTTGACAATCTAATAAAGTTTTGTCCAACAAAAGAAGAAGCAGAATTGCTCAAGGGCTTCATTGGAAATAAAGAAACTTTGGGGCGGTGTGAGCAGTTCTTTTTAGAACTGTTGAAAGTTCCTCGAGTGGAGACTAAGCTAAGGGTTTTCTCATTCAAGATTCAGTTTCACTCCCAGGTTACTGATCTTAGAAGAGGTTTGAATACCATACATAGTGCAACCAATGAGGCAAGTCGATTTTTCGTGCAAATAGTTAGAGGCTCGACTAAACTAAAAAGAATCATGCAAACTATACTTTCCTTGGGCAATGCATTGAACCACGGGACTGCAAGGGGCTCAGCTATCGGGTTTCACCTGGATAGTCTTCTGAAACTCACTGACACTAGATCCCGTAACATCTTTATCTTTGTGCTTGCCGAGAAGCTTCCAGGGCTACTTAATTTTCCAAAAGACATGGTGAGCTTGGAGGCTGCAACAAATATTCAACTAAAATACCTGGCAGAGGAGATGCAAGCAACTAGCAAAGGGTTGGAGAAAGTTGTACAAGAGTTTACTGCATCTGAAACTGATTGTCAAATATCAAAACACTTTCATATGAACTTGAAAGAGTTTCTTAGCGTTGCTGAAGGAGAAGTAAGGTCCTTGGCTTCTCTCTATTCGACTGTGGGTGGAAGCGCCGATGCTTTGGCGTTATACTTCGGAGAAGATCCAGCTCGTGTCCCATTTGAGCAAGTTGTATCCACTCTGCAAAACTTTGTTAGAATATTTGTGCGGTCACACGAGGAAAACTGCAAGCAAGTTGAGTTTGAGAAGAAGAGAGCACAGAAGGAAGCGGAGAACGAAAAACTTAAGAAAGGTGTTTACAATGAGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0009524 GO:0009555 GO:0009556 GO:0009574 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0030029 GO:0030036 GO:0032501 GO:0032502 GO:0034293 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043934 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044877 GO:0048229 GO:0048236 GO:0048856 GO:0048869 GO:0051015 GO:0051017 GO:0051321 GO:0051704 GO:0061572 GO:0071840 GO:0097435 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FH2 PTEN_C2

Protein Analysis

488

Amino Acids

54.15

Weight (kDa)

8.95

Isoelectric Point (pI)

37.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	83 - 453	1.5e-90	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003178)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25050 AT3G32400 AT3G32400 AT3G32410
fragaria_vesca	FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080 FvH4_2g41080
malus_domestica	MD00G1143700.v1.1 MD15G1039700.v1.1
prunus_persica	Prupe.1G393200_v2.0.a1
pyrus_communis	pycom08g04710 pycom15g03710
rosa_chinensis	RchiOBHm_Chr6g0299461
rosa_laevigata	RLG00000011429
rosa_multiflora	Rmu_co8403397.1_g000001 Rmu_sc0001184.1_g000009
rosa_roxburghii	Rroxscaffold_7G00168650
rosa_rugosa	Rorug06G0288500 Rorug06G0288600 Rorug06G0288700 Rorug06G0288800 Rorug06G0288900 Rorug06G0288900
rosa_samantha	Rh6AG400300 Rh6BG409000 Rh6CG414400 Rh6DG401400
rosa_wichuraiana	Rw6G035040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	4 cut(s)	53, 676, 1363, 1424
AclWI	GGATC	2 cut(s)	972, 1292
AcuI	CTGAAG	2 cut(s)	339, 1236
AfaI	GTAC	1 cut(s)	1134
AfiI	CCNNNNNNNGG	1 cut(s)	1093
AflII	CTTAAG	1 cut(s)	1439
AgsI	TTSAA	8 cut(s)	214, 265, 706, 748, 793, 907, 1076, 1195
AjiI	CACGTC	1 cut(s)	443
AjnI	CCWGG	4 cut(s)	765, 945, 1018, 1086
Alw21I	GWGCWC	2 cut(s)	483, 1414
Alw26I	GTCTC	1 cut(s)	716
AlwI	GGATC	2 cut(s)	972, 1292
Ama87I	CYCGRG	1 cut(s)	714
AoxI	GGCC	3 cut(s)	32, 255, 436
ApeKI	GCWGC	4 cut(s)	7, 341, 469, 1061
Asp700I	GAANNNNTTC	1 cut(s)	383
AspLEI	GCGC	2 cut(s)	325, 1268
AspS9I	GGNCC	2 cut(s)	32, 1230
AsuHPI	GGTGA	3 cut(s)	62, 935, 1060
AvaI	CYCGRG	1 cut(s)	714
AvaII	GGWCC	1 cut(s)	1230
BaeGI	GKGCMC	1 cut(s)	448
BanII	GRGCYC	3 cut(s)	193, 483, 929
BauI	CACGAG	2 cut(s)	1305, 1370
BbsI	GAAGAC	1 cut(s)	947
Bbv12I	GWGCWC	2 cut(s)	483, 1414
BbvI	GCAGC	3 cut(s)	328, 481, 1048
BciT130I	CCWGG	4 cut(s)	767, 947, 1020, 1088
BciVI	GTATCC	1 cut(s)	1339
BclI	TGATCA	1 cut(s)	586
BcoDI	GTCTC	1 cut(s)	716
BfaI	CTAG	3 cut(s)	291, 975, 1110
BfoI	RGCGCY	1 cut(s)	1269
BfrI	CTTAAG	1 cut(s)	1439
BfuI	GTATCC	1 cut(s)	1339
BisI	GCNGC	4 cut(s)	8, 342, 470, 1062
BlpI	GCTNAGC	1 cut(s)	928
BlsI	GCNGC	4 cut(s)	9, 343, 471, 1063
Bme1390I	CCNGG	4 cut(s)	767, 947, 1020, 1088
Bme18I	GGWCC	1 cut(s)	1230
BmeT110I	CYCGRG	1 cut(s)	714
BmgBI	CACGTC	1 cut(s)	443
BmgT120I	GGNCC	2 cut(s)	32, 1230
BmiI	GGNNCC	1 cut(s)	190
BmrFI	CCNGG	4 cut(s)	767, 947, 1020, 1088
BmsI	GCATC	3 cut(s)	1089, 1157, 1261
BpiI	GAAGAC	1 cut(s)	947
BpmI	CTGGAG	1 cut(s)	336
Bpu10I	CCTNAGC	1 cut(s)	731
Bpu1102I	GCTNAGC	1 cut(s)	928
BpuEI	CTTGAG	1 cut(s)	626
BsaBI	GATNNNNATC	1 cut(s)	585
BsaJI	CCNNGG	5 cut(s)	765, 891, 910, 1019, 1233
BsaXI	ACNNNNNCTCC	4 cut(s)	127, 133, 157, 163
Bsc4I	CCNNNNNNNGG	1 cut(s)	1093
Bse3DI	GCAATG	1 cut(s)	904
Bse8I	GATNNNNATC	1 cut(s)	585
BseBI	CCWGG	4 cut(s)	767, 947, 1020, 1088
BseDI	CCNNGG	5 cut(s)	765, 891, 910, 1019, 1233
BseGI	GGATG	1 cut(s)	571
BseJI	GATNNNNATC	1 cut(s)	585
BseLI	CCNNNNNNNGG	1 cut(s)	1093
BseMI	GCAATG	1 cut(s)	904
BseMII	CTCAG	4 cut(s)	25, 360, 402, 942
BseRI	GAGGAG	4 cut(s)	27, 84, 210, 1109
BseSI	GKGCMC	1 cut(s)	448
BseXI	GCAGC	3 cut(s)	328, 481, 1048
BsgI	GTGCAG	2 cut(s)	63, 488
BshFI	GGCC	3 cut(s)	34, 257, 438
BsiHKAI	GWGCWC	2 cut(s)	483, 1414
BsiHKCI	CYCGRG	1 cut(s)	714
BslFI	GGGAC	2 cut(s)	928, 1295
BslI	CCNNNNNNNGG	1 cut(s)	1093
BsmAI	GTCTC	1 cut(s)	716
BsmFI	GGGAC	2 cut(s)	928, 1295
BsmI	GAATGC	1 cut(s)	169
BsnI	GGCC	3 cut(s)	34, 257, 438
BsoBI	CYCGRG	1 cut(s)	714
Bsp1286I	GDGCHC	5 cut(s)	193, 448, 483, 929, 1414
Bsp1407I	TGTACA	1 cut(s)	1132
Bsp143I	GATC	5 cut(s)	504, 586, 775, 977, 1297
Bsp1720I	GCTNAGC	1 cut(s)	928
BspACI	CCGC	4 cut(s)	53, 676, 1363, 1424
BspANI	GGCC	3 cut(s)	34, 257, 438
BspCNI	CTCAG	4 cut(s)	24, 361, 401, 941
BspLI	GGNNCC	1 cut(s)	190
BspPI	GGATC	2 cut(s)	972, 1292
BspTI	CTTAAG	1 cut(s)	1439
BsrDI	GCAATG	1 cut(s)	904
BsrGI	TGTACA	1 cut(s)	1132
BssECI	CCNNGG	5 cut(s)	765, 891, 910, 1019, 1233
BssMI	GATC	5 cut(s)	504, 586, 775, 977, 1297
BssSI	CACGAG	2 cut(s)	1305, 1370
BssT1I	CCWWGG	2 cut(s)	891, 1233
Bst2BI	CACGAG	2 cut(s)	1305, 1370
Bst2UI	CCWGG	4 cut(s)	767, 947, 1020, 1088
Bst4CI	ACNGT	5 cut(s)	270, 413, 500, 702, 1255
Bst6I	CTCTTC	4 cut(s)	63, 389, 778, 1400
BstAFI	CTTAAG	1 cut(s)	1439
BstAUI	TGTACA	1 cut(s)	1132
BstC8I	GCNNGC	3 cut(s)	1005, 1104, 1385
BstDEI	CTNAG	9 cut(s)	11, 369, 388, 401, 726, 731, 779, 928, 1205
BstDSI	CCRYGG	1 cut(s)	910
BstENI	CCTNNNNNAGG	1 cut(s)	1091
BstF5I	GGATG	1 cut(s)	571
BstH2I	RGCGCY	1 cut(s)	1269
BstHHI	GCGC	2 cut(s)	325, 1268
BstKTI	GATC	5 cut(s)	507, 589, 778, 980, 1300
BstMAI	GTCTC	1 cut(s)	716
BstMBI	GATC	5 cut(s)	504, 586, 775, 977, 1297
BstMWI	GCNNNNNNNGC	4 cut(s)	347, 478, 682, 1021
BstNI	CCWGG	4 cut(s)	767, 947, 1020, 1088
BstSCI	CCNGG	4 cut(s)	765, 945, 1018, 1086
BstSLI	GKGCMC	1 cut(s)	448
BstV1I	GCAGC	3 cut(s)	328, 481, 1048
BstV2I	GAAGAC	1 cut(s)	947
BstX2I	RGATCY	2 cut(s)	977, 1297
BstYI	RGATCY	2 cut(s)	977, 1297
BsuI	GTATCC	1 cut(s)	1339
BsuRI	GGCC	3 cut(s)	34, 257, 438
BtgI	CCRYGG	1 cut(s)	910
BtrI	CACGTC	1 cut(s)	443
BtsCI	GGATG	1 cut(s)	571
BtsI	GCAGTG	2 cut(s)	303, 399
BtsIMutI	CAGTG	5 cut(s)	303, 399, 418, 579, 966
Cac8I	GCNNGC	3 cut(s)	1005, 1104, 1385
CfoI	GCGC	2 cut(s)	325, 1268
Cfr13I	GGNCC	2 cut(s)	32, 1230
Csp6I	GTAC	1 cut(s)	1133
CviAII	CATG	3 cut(s)	508, 874, 1045
CviQI	GTAC	1 cut(s)	1133
DdeI	CTNAG	9 cut(s)	11, 369, 388, 401, 726, 731, 779, 928, 1205
DpnI	GATC	5 cut(s)	506, 588, 777, 979, 1299
DpnII	GATC	5 cut(s)	504, 586, 775, 977, 1297
Eam1104I	CTCTTC	4 cut(s)	63, 389, 778, 1400
EarI	CTCTTC	4 cut(s)	63, 389, 778, 1400
EciI	GGCGGA	1 cut(s)	42
Ecl136II	GAGCTC	1 cut(s)	481
Eco130I	CCWWGG	2 cut(s)	891, 1233
Eco24I	GRGCYC	3 cut(s)	193, 483, 929
Eco47I	GGWCC	1 cut(s)	1230
Eco53kI	GAGCTC	1 cut(s)	481
Eco57I	CTGAAG	2 cut(s)	339, 1236
Eco88I	CYCGRG	1 cut(s)	714
EcoICRI	GAGCTC	1 cut(s)	481
EcoNI	CCTNNNNNAGG	1 cut(s)	1091
EcoO109I	RGGNCCY	2 cut(s)	32, 1230
EcoRII	CCWGG	4 cut(s)	765, 945, 1018, 1086
EcoT14I	CCWWGG	2 cut(s)	891, 1233
EcoT22I	ATGCAT	1 cut(s)	904
EcoT38I	GRGCYC	3 cut(s)	193, 483, 929
ErhI	CCWWGG	2 cut(s)	891, 1233
FaeI	CATG	3 cut(s)	511, 877, 1048
FalI	AAGNNNNNCTT	4 cut(s)	245, 277, 1188, 1220
FaqI	GGGAC	2 cut(s)	928, 1295
FatI	CATG	3 cut(s)	507, 873, 1044
FauNDI	CATATG	1 cut(s)	1185
FbaI	TGATCA	1 cut(s)	586
Fnu4HI	GCNGC	4 cut(s)	8, 342, 470, 1062
FokI	GGATG	1 cut(s)	578
FriOI	GRGCYC	3 cut(s)	193, 483, 929
Fsp4HI	GCNGC	4 cut(s)	8, 342, 470, 1062
FspBI	CTAG	3 cut(s)	291, 975, 1110
GlaI	GCGC	2 cut(s)	324, 1267
GluI	GCNGC	4 cut(s)	8, 342, 470, 1062
GsuI	CTGGAG	1 cut(s)	336
HaeII	RGCGCY	1 cut(s)	1269
HaeIII	GGCC	3 cut(s)	34, 257, 438
HhaI	GCGC	2 cut(s)	325, 1268
Hin1II	CATG	3 cut(s)	511, 877, 1048
Hin6I	GCGC	2 cut(s)	323, 1266
HinP1I	GCGC	2 cut(s)	323, 1266
HincII	GTYRAC	1 cut(s)	595
HindII	GTYRAC	1 cut(s)	595
HindIII	AAGCTT	2 cut(s)	284, 1013
HinfI	GANTC	4 cut(s)	397, 569, 751, 870
HphI	GGTGA	3 cut(s)	62, 935, 1060
Hpy166II	GTNNAC	3 cut(s)	595, 1143, 1453
Hpy188I	TCNGA	5 cut(s)	334, 370, 960, 1153, 1292
Hpy188III	TCNNGA	2 cut(s)	353, 748
Hpy8I	GTNNAC	3 cut(s)	595, 1143, 1453
Hpy99I	CGWCG	1 cut(s)	444
HpyAV	CCTTC	3 cut(s)	23, 1211, 1411
HpyCH4III	ACNGT	5 cut(s)	270, 413, 500, 702, 1255
HpyCH4IV	ACGT	1 cut(s)	442
HpyF10VI	GCNNNNNNNGC	4 cut(s)	347, 478, 682, 1021
HpyF3I	CTNAG	9 cut(s)	11, 369, 388, 401, 726, 731, 779, 928, 1205
HpySE526I	ACGT	1 cut(s)	442
Hsp92II	CATG	3 cut(s)	511, 877, 1048
HspAI	GCGC	2 cut(s)	323, 1266
Ksp22I	TGATCA	1 cut(s)	586
Kzo9I	GATC	5 cut(s)	504, 586, 775, 977, 1297
LmnI	GCTCC	3 cut(s)	4, 188, 355
Lsp1109I	GCAGC	3 cut(s)	328, 481, 1048
LweI	GCATC	3 cut(s)	1089, 1157, 1261
MaeI	CTAG	3 cut(s)	291, 975, 1110
MaeII	ACGT	1 cut(s)	442
MaeIII	GTNAC	3 cut(s)	769, 983, 1365
MalI	GATC	5 cut(s)	506, 588, 777, 979, 1299
MboI	GATC	5 cut(s)	504, 586, 775, 977, 1297
MboII	GAAGA	9 cut(s)	50, 81, 376, 638, 795, 947, 1307, 1414, 1417
MflI	RGATCY	2 cut(s)	977, 1297
MhlI	GDGCHC	5 cut(s)	193, 448, 483, 929, 1414
MluCI	AATT	4 cut(s)	122, 175, 635, 1030
MlyI	GAGTC	1 cut(s)	578
MmeI	TCCRAC	2 cut(s)	641, 1101
Mph1103I	ATGCAT	1 cut(s)	904
MroXI	GAANNNNTTC	1 cut(s)	383
MseI	TTAA	5 cut(s)	231, 281, 522, 1029, 1440
MspA1I	CMGCKG	1 cut(s)	472
MspCI	CTTAAG	1 cut(s)	1439
MspR9I	CCNGG	4 cut(s)	767, 947, 1020, 1088
Mva1269I	GAATGC	1 cut(s)	169
MvaI	CCWGG	4 cut(s)	767, 947, 1020, 1088
MwoI	GCNNNNNNNGC	4 cut(s)	347, 478, 682, 1021
NdeI	CATATG	1 cut(s)	1185
NdeII	GATC	5 cut(s)	504, 586, 775, 977, 1297
NlaIII	CATG	3 cut(s)	511, 877, 1048
NlaIV	GGNNCC	1 cut(s)	190
NmeAIII	GCCGAG	1 cut(s)	1033
NmuCI	GTSAC	1 cut(s)	1365
NsiI	ATGCAT	1 cut(s)	904
PaeR7I	CTCGAG	1 cut(s)	714
PctI	GAATGC	1 cut(s)	169
PdmI	GAANNNNTTC	1 cut(s)	383
PfeI	GAWTC	3 cut(s)	397, 751, 870
PkrI	GCNGC	4 cut(s)	9, 343, 471, 1063
PleI	GAGTC	1 cut(s)	577
PpsI	GAGTC	1 cut(s)	577
PpuMI	RGGWCCY	1 cut(s)	1230
Psp124BI	GAGCTC	1 cut(s)	483
Psp5II	RGGWCCY	1 cut(s)	1230
Psp6I	CCWGG	4 cut(s)	765, 945, 1018, 1086
PspGI	CCWGG	4 cut(s)	765, 945, 1018, 1086
PspN4I	GGNNCC	1 cut(s)	190
PspPI	GGNCC	2 cut(s)	32, 1230
PspPPI	RGGWCCY	1 cut(s)	1230
PspXI	VCTCGAGB	1 cut(s)	714
PsuI	RGATCY	2 cut(s)	977, 1297
PvuII	CAGCTG	1 cut(s)	472
RsaI	GTAC	1 cut(s)	1134
RsaNI	GTAC	1 cut(s)	1133
SacI	GAGCTC	1 cut(s)	483
SaqAI	TTAA	5 cut(s)	231, 281, 522, 1029, 1440
SatI	GCNGC	4 cut(s)	8, 342, 470, 1062
Sau3AI	GATC	5 cut(s)	504, 586, 775, 977, 1297
Sau96I	GGNCC	2 cut(s)	32, 1230
SchI	GAGTC	1 cut(s)	578
ScrFI	CCNGG	4 cut(s)	767, 947, 1020, 1088
SduI	GDGCHC	5 cut(s)	193, 448, 483, 929, 1414
SfaNI	GCATC	3 cut(s)	1089, 1157, 1261
Sfr274I	CTCGAG	1 cut(s)	714
SinI	GGWCC	1 cut(s)	1230
SlaI	CTCGAG	1 cut(s)	714
SmlI	CTYRAG	3 cut(s)	641, 714, 1439
SmoI	CTYRAG	3 cut(s)	641, 714, 1439
Sse9I	AATT	4 cut(s)	122, 175, 635, 1030
SsiI	CCGC	4 cut(s)	53, 676, 1363, 1424
SspI	AATATT	2 cut(s)	1072, 1356
SspMI	CTAG	3 cut(s)	291, 975, 1110
SstI	GAGCTC	1 cut(s)	483
StyD4I	CCNGG	4 cut(s)	765, 945, 1018, 1086
StyI	CCWWGG	2 cut(s)	891, 1233
TaaI	ACNGT	5 cut(s)	270, 413, 500, 702, 1255
TaiI	ACGT	1 cut(s)	445
TaqI	TCGA	4 cut(s)	715, 826, 854, 1250
TasI	AATT	4 cut(s)	122, 175, 635, 1030
TatI	WGTACW	1 cut(s)	1132
TfiI	GAWTC	3 cut(s)	397, 751, 870
Tru1I	TTAA	5 cut(s)	231, 281, 522, 1029, 1440
Tru9I	TTAA	5 cut(s)	231, 281, 522, 1029, 1440
TscAI	CASTG	5 cut(s)	303, 399, 418, 579, 973
TseFI	GTSAC	1 cut(s)	1365
TseI	GCWGC	4 cut(s)	7, 341, 469, 1061
Tsp45I	GTSAC	1 cut(s)	1365
TspDTI	ATGAA	5 cut(s)	75, 351, 640, 1172, 1202
TspRI	CASTG	5 cut(s)	303, 399, 418, 579, 973
Vha464I	CTTAAG	1 cut(s)	1439
VpaK11BI	GGWCC	1 cut(s)	1230
XagI	CCTNNNNNAGG	1 cut(s)	1091
XcmI	CCANNNNNNNNNTGG	1 cut(s)	202
XhoI	CTCGAG	1 cut(s)	714
XmnI	GAANNNNTTC	1 cut(s)	383
XspI	CTAG	3 cut(s)	291, 975, 1110
Zsp2I	ATGCAT	1 cut(s)	904

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.