Rorug06G0288900

C2 domain of PTEN tumour-suppressor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003178
Physical Location & Seq
Reverse (-)
43907464 .. 43910784
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0288900.1

Sequence Viewer

Length: 861 bp
ATGGAGGAGAAAGCAATCAGATTTAGTTGGGGTTTTGCCAAAGATTTCAAGGGCTTTCCACACCCAGAAGACAACTTCAGATCAGCTACTGGGAATGGTGCAGTCTGGTGTTGCAAGGCAGTCTACTGCTTACTCAAGTCATTTGACCAGGGTGAGCGCATAGTTGGAACTGATGGAATTTCGGTTCTGTCTGGAAGGAATTTTACTAGAGAATTAATCAATACTAAGTGGGCTCCATTACAACTTACGAGAAACTATGGAGCAAACAATGTTGTTCCTCGCTTTAGTAGAACCATGTCTTCAGAAGCTTCACAGAAACCGCGAAGAAATCGATCTGAGACTAAGAAAGACATATCCACTGTGGAGGAGCCTTTTGATGATGCTCCTACGTACAATATCCCTCAGAAGCCACTGACGTTTGTTGAGGGGGCATCCTATAGTGTTGTCATTCTTATCGGGTTTGGAATTGCTGGCTTGGCAGCATATGCCGTTTTTAAGGAGCTTATTTTTGAACCAAAAGAGTACAAAATCTTCAACAAGGCGCTTAAACGGATTCAAGATGATGCTCAGGTTAGAATGAGGGTTGGATACCCTATCACAGGATATGGTCAAGAAAGTAGAAATCGTGCTGCTCGACAACGCATTCCAAACAGAATGTGGACTGATGAAGATGGTTTAGAGCACGTAGAGGTCAATTTCTATGTCCGTGGGCCGCACGGAGCTGGGAAAGTATTCGCTGAGATGTTCAAAGACGCAGACAAGAGGTGGAAATACACATATCTGGTTGTCGAGACAACATCACCTTCCCAATCACGTCTGATCCTAGAGTCCTATCTGCCGGAGTACAAGCCAGCCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FH2 PTEN_C2

Protein Analysis

286

Amino Acids

32.62

Weight (kDa)

9.26

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIM21 PF08294 143 - 275 2.2e-24 TIM21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 322
AciI CCGC 2 cut(s) 320, 713
AclWI GGATC 1 cut(s) 814
AcsI RAATTY 2 cut(s) 177, 199
AcuI CTGAAG 2 cut(s) 61, 285
AfaI GTAC 3 cut(s) 392, 524, 845
AfiI CCNNNNNNNGG 1 cut(s) 599
AgsI TTSAA 5 cut(s) 49, 512, 535, 557, 748
AjiI CACGTC 1 cut(s) 815
AjnI CCWGG 1 cut(s) 147
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 4 cut(s) 86, 308, 502, 722
AluI AGCT 4 cut(s) 86, 308, 502, 722
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 2 cut(s) 332, 785
AlwI GGATC 1 cut(s) 814
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 1 cut(s) 710
ApeKI GCWGC 2 cut(s) 479, 629
ApoI RAATTY 2 cut(s) 177, 199
AseI ATTAAT 1 cut(s) 215
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 2 cut(s) 159, 544
AspS9I GGNCC 1 cut(s) 710
AsuHPI GGTGA 2 cut(s) 164, 792
BanII GRGCYC 1 cut(s) 235
BarI GAAGNNNNNNTAC 4 cut(s) 187, 219, 515, 547
BbsI GAAGAC 2 cut(s) 75, 291
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 2 cut(s) 491, 616
BccI CCATC 2 cut(s) 167, 665
BceAI ACGGC 1 cut(s) 473
BciT130I CCWGG 1 cut(s) 149
BciVI GTATCC 1 cut(s) 581
BcoDI GTCTC 2 cut(s) 332, 785
BfaI CTAG 3 cut(s) 207, 824, 859
BfmI CTRYAG 1 cut(s) 436
BfoI RGCGCY 1 cut(s) 545
BfuI GTATCC 1 cut(s) 581
BisI GCNGC 3 cut(s) 480, 630, 713
BlsI GCNGC 3 cut(s) 481, 631, 714
Bme1390I CCNGG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 815
BmgT120I GGNCC 1 cut(s) 710
BmiI GGNNCC 2 cut(s) 234, 369
BmrFI CCNGG 1 cut(s) 149
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 3 cut(s) 370, 440, 553
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 75, 291
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 331
BsaAI YACGTR 2 cut(s) 390, 685
BsaJI CCNNGG 2 cut(s) 148, 706
Bsc4I CCNNNNNNNGG 1 cut(s) 599
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 149
BseCI ATCGAT 1 cut(s) 331
BseDI CCNNGG 2 cut(s) 148, 706
BseGI GGATG 1 cut(s) 431
BseLI CCNNNNNNNGG 1 cut(s) 599
BseMII CTCAG 4 cut(s) 327, 416, 581, 729
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 2 cut(s) 20, 380
BseXI GCAGC 2 cut(s) 491, 616
BseYI CCCAGC 1 cut(s) 722
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 1 cut(s) 712
BshVI ATCGAT 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 684
BsiSI CCGG 1 cut(s) 839
BslI CCNNNNNNNGG 1 cut(s) 599
BsmAI GTCTC 2 cut(s) 332, 785
BsmI GAATGC 1 cut(s) 642
BsnI GGCC 1 cut(s) 712
Bsp1286I GDGCHC 2 cut(s) 235, 684
Bsp143I GATC 3 cut(s) 80, 332, 819
BspACI CCGC 2 cut(s) 320, 713
BspANI GGCC 1 cut(s) 712
BspCNI CTCAG 4 cut(s) 328, 415, 580, 730
BspDI ATCGAT 1 cut(s) 331
BspFNI CGCG 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 234, 369
BspPI GGATC 1 cut(s) 814
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 2 cut(s) 148, 706
BssMI GATC 3 cut(s) 80, 332, 819
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 361
BstAPI GCANNNNNTGC 1 cut(s) 485
BstBAI YACGTR 2 cut(s) 390, 685
BstC8I GCNNGC 2 cut(s) 472, 852
BstDEI CTNAG 6 cut(s) 225, 336, 342, 402, 567, 738
BstDSI CCRYGG 1 cut(s) 706
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 1 cut(s) 431
BstFNI CGCG 1 cut(s) 322
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 2 cut(s) 159, 544
BstKTI GATC 3 cut(s) 83, 335, 822
BstMAI GTCTC 2 cut(s) 332, 785
BstMBI GATC 3 cut(s) 80, 332, 819
BstMWI GCNNNNNNNGC 2 cut(s) 476, 485
BstNI CCWGG 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 147
BstSFI CTRYAG 1 cut(s) 436
BstSNI TACGTA 1 cut(s) 390
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 2 cut(s) 491, 616
BstV2I GAAGAC 2 cut(s) 75, 291
Bsu15I ATCGAT 1 cut(s) 331
BsuI GTATCC 1 cut(s) 581
BsuRI GGCC 1 cut(s) 712
BsuTUI ATCGAT 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 706
BtrI CACGTC 1 cut(s) 815
BtsCI GGATG 1 cut(s) 431
BtsIMutI CAGTG 2 cut(s) 357, 410
Cac8I GCNNGC 2 cut(s) 472, 852
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 2 cut(s) 159, 544
Cfr13I GGNCC 1 cut(s) 710
ClaI ATCGAT 1 cut(s) 331
CseI GACGC 1 cut(s) 761
Csp6I GTAC 3 cut(s) 391, 523, 844
CviAII CATG 1 cut(s) 295
CviQI GTAC 3 cut(s) 391, 523, 844
DdeI CTNAG 6 cut(s) 225, 336, 342, 402, 567, 738
DpnI GATC 3 cut(s) 82, 334, 821
DpnII GATC 3 cut(s) 80, 332, 819
Eco105I TACGTA 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 61, 285
EcoNI CCTNNNNNAGG 1 cut(s) 597
EcoRII CCWGG 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 1 cut(s) 298
FatI CATG 1 cut(s) 294
FauNDI CATATG 1 cut(s) 484
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 480, 630, 713
FokI GGATG 1 cut(s) 418
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 3 cut(s) 480, 630, 713
FspBI CTAG 3 cut(s) 207, 824, 859
GlaI GCGC 2 cut(s) 158, 543
GluI GCNGC 3 cut(s) 480, 630, 713
GsaI CCCAGC 1 cut(s) 726
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 1 cut(s) 712
HapII CCGG 1 cut(s) 839
HgaI GACGC 1 cut(s) 761
HhaI GCGC 2 cut(s) 159, 544
Hin1II CATG 1 cut(s) 298
Hin6I GCGC 2 cut(s) 157, 542
HinP1I GCGC 2 cut(s) 157, 542
HindIII AAGCTT 1 cut(s) 306
HinfI GANTC 2 cut(s) 553, 827
HpaII CCGG 1 cut(s) 839
HphI GGTGA 2 cut(s) 164, 792
Hpy166II GTNNAC 2 cut(s) 124, 660
Hpy188I TCNGA 6 cut(s) 20, 80, 304, 337, 405, 819
Hpy188III TCNNGA 4 cut(s) 192, 557, 611, 790
Hpy8I GTNNAC 2 cut(s) 124, 660
HpyAV CCTTC 2 cut(s) 189, 813
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 4 cut(s) 389, 416, 684, 814
HpyCH4V TGCA 2 cut(s) 101, 114
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 485
HpyF3I CTNAG 6 cut(s) 225, 336, 342, 402, 567, 738
HpySE526I ACGT 4 cut(s) 389, 416, 684, 814
Hsp92II CATG 1 cut(s) 298
HspAI GCGC 2 cut(s) 157, 542
Kzo9I GATC 3 cut(s) 80, 332, 819
LmnI GCTCC 6 cut(s) 238, 260, 367, 388, 499, 719
Lsp1109I GCAGC 2 cut(s) 491, 616
LweI GCATC 3 cut(s) 370, 440, 553
MaeI CTAG 3 cut(s) 207, 824, 859
MaeII ACGT 4 cut(s) 389, 416, 684, 814
MalI GATC 3 cut(s) 82, 334, 821
MboI GATC 3 cut(s) 80, 332, 819
MboII GAAGA 5 cut(s) 80, 291, 336, 523, 680
MhlI GDGCHC 2 cut(s) 235, 684
MluCI AATT 5 cut(s) 177, 199, 212, 465, 694
MlyI GAGTC 1 cut(s) 836
MmeI TCCRAC 2 cut(s) 145, 565
MnlI CCTC 7 cut(s) 288, 358, 411, 418, 573, 682, 756
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 3 cut(s) 215, 495, 546
MspI CCGG 1 cut(s) 839
MspR9I CCNGG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 642
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 476, 485
NdeI CATATG 1 cut(s) 484
NdeII GATC 3 cut(s) 80, 332, 819
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 2 cut(s) 234, 369
PcsI WCGNNNNNNNCGW 1 cut(s) 631
PctI GAATGC 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 1 cut(s) 553
PkrI GCNGC 3 cut(s) 481, 631, 714
PleI GAGTC 1 cut(s) 835
PpsI GAGTC 1 cut(s) 835
Ppu21I YACGTR 2 cut(s) 390, 685
PshBI ATTAAT 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 722
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 234, 369
PspPI GGNCC 1 cut(s) 710
PstNI CAGNNNCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 392, 524, 845
RsaNI GTAC 3 cut(s) 391, 523, 844
SaqAI TTAA 3 cut(s) 215, 495, 546
SatI GCNGC 3 cut(s) 480, 630, 713
Sau3AI GATC 3 cut(s) 80, 332, 819
Sau96I GGNCC 1 cut(s) 710
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 1 cut(s) 149
SduI GDGCHC 2 cut(s) 235, 684
SfaNI GCATC 3 cut(s) 370, 440, 553
SfcI CTRYAG 1 cut(s) 436
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
SnaBI TACGTA 1 cut(s) 390
Sse9I AATT 5 cut(s) 177, 199, 212, 465, 694
SsiI CCGC 2 cut(s) 320, 713
SspMI CTAG 3 cut(s) 207, 824, 859
StyD4I CCNGG 1 cut(s) 147
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 4 cut(s) 392, 419, 687, 817
TaqI TCGA 3 cut(s) 331, 634, 789
TasI AATT 5 cut(s) 177, 199, 212, 465, 694
TatI WGTACW 2 cut(s) 522, 843
TauI GCSGC 1 cut(s) 715
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 3 cut(s) 215, 495, 546
Tru9I TTAA 3 cut(s) 215, 495, 546
TscAI CASTG 2 cut(s) 364, 417
TseI GCWGC 2 cut(s) 479, 629
TspDTI ATGAA 1 cut(s) 681
TspGWI ACGGA 3 cut(s) 565, 695, 732
TspRI CASTG 2 cut(s) 364, 417
VspI ATTAAT 1 cut(s) 215
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 2 cut(s) 177, 199
XcmI CCANNNNNNNNNTGG 1 cut(s) 654
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 3 cut(s) 207, 824, 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.