Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011443

Physical Location & Seq

Genomic Coordinates Reverse (-)

10679524 .. 10682054

Gene Structure 2531 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G25100.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 891 bp

ATGGAATCTGAATGTTTGACACCAGAATGGGCATCTCAACCTTGTCTAATGGGTATCGATGAAGCCGGAAGGGGTCCTGTTCTAGGTCCTATGGTTTATGGATGTATGTATTGTCCCATTTCCTATCAGTCGTCTCTTGCTTCTCTCCATTTTGCAGATTCGAAGACTCTAAAAGAGGAGAAAAGAGAAGAGTTATATGAAAGCTTAAAGCTTGACAAGTCACTTGGATGGGCTGTTGATGTCATTGATCCTAGGGAACTCTCAGCCAAGATGCTCGCAAAAAACAAGACTAATTTAAATGAGATTTCACATAACTCTGCAATGGGGCTTATCAAAAGGGTACTAGACATGGGAGTTCTTCTGACAGAGGCTTATCTGGATACCGTGGGAGATCCCGACAAGTACAGAATAAAATTGTCTGAGAGGTTTCCTTCGATCAAGTTCGTCGTTTCAAAGAAAGCTGACAGTCTATTTCCGATTGTTAGTGGGGCAAGTATTGTGGCTAAGGTGACAAGAGACAGAGCGTTGAAAGAATGGTTGGTAGAGGAAACCGGAGAAGATATAAATAGAAACTTTGGTTCTGGATATCCTGGAGATCCTGAGACAAAGGCGTGGTTAGTGCAACACAAACATTCTGTGTTTGGATTCCCATCACTGGTCCGCTTCAGTTGGGGAACTTGCACTACACATTTGAAGGGCGAGGTTGAAGTTGCATGGGAAGCAGATGAAAATGAAGAGAGTGGTAATGGAAGTAGCAGCAAACGACAAGCGAAACTAAGCAGTTTTGGGTTCAAAACATGCGAGAAAAGAAGCGAAGAAATCGAGTCGAGTGGAAAAGGACGCTGCAAATTTCTCCAAGCTCGCAAAATCCAACAACTCACTCAGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004523 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006273 GO:0006281 GO:0006298 GO:0006401 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019439 GO:0022616 GO:0032299 GO:0032991 GO:0033554 GO:0033567 GO:0034641 GO:0034645 GO:0034655 GO:0043137 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0046483 GO:0046700 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 map03030

Pfam Domains

Protein Families

RNase_HII

Protein Analysis

296

Amino Acids

33.08

Weight (kDa)

6.28

Isoelectric Point (pI)

38.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNase_HII	PF01351	17 - 208	3.1e-40	Ribonuclease HII

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25100 AT2G25100
fragaria_vesca	FvH4_3g27560 FvH4_3g27560
malus_domestica	MD02G1261600.v1.1
prunus_persica	Prupe.2G069600_v2.0.a1 Prupe.2G069600_v2.0.a1
pyrus_communis	pycom02g22370
rosa_chinensis	RchiOBHm_Chr5g0049931
rosa_laevigata	RLG00000034666
rosa_roxburghii	Rroxscaffold_1G00031380
rosa_rugosa	Rorug05G0254300 Rorug05G0254400 Rorug05G0254500 Rorug05G0254600
rosa_samantha	Rh5AG328700 Rh5BG339300 Rh5CG365100 Rh5DG351700
rosa_wichuraiana	Rw5G031050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	661
AclWI	GGATC	3 cut(s)	242, 386, 590
AcsI	RAATTY	1 cut(s)	848
AcuI	CTGAAG	1 cut(s)	649
AfaI	GTAC	2 cut(s)	342, 404
AfiI	CCNNNNNNNGG	2 cut(s)	83, 655
AgsI	TTSAA	5 cut(s)	453, 529, 694, 707, 793
AjnI	CCWGG	1 cut(s)	589
AjuI	GAANNNNNNNTTGG	2 cut(s)	521, 553
AluBI	AGCT	4 cut(s)	204, 211, 461, 860
AluI	AGCT	4 cut(s)	204, 211, 461, 860
Alw26I	GTCTC	3 cut(s)	138, 510, 596
AlwI	GGATC	3 cut(s)	242, 386, 590
ApeKI	GCWGC	2 cut(s)	756, 843
ApoI	RAATTY	1 cut(s)	848
AspA2I	CCTAGG	1 cut(s)	251
AspS9I	GGNCC	3 cut(s)	74, 86, 658
AsuHPI	GGTGA	1 cut(s)	520
AsuII	TTCGAA	1 cut(s)	161
AvaII	GGWCC	3 cut(s)	74, 86, 658
AvrII	CCTAGG	1 cut(s)	251
BbsI	GAAGAC	1 cut(s)	170
BbvI	GCAGC	2 cut(s)	768, 830
BccI	CCATC	2 cut(s)	222, 658
BciT130I	CCWGG	1 cut(s)	591
BciVI	GTATCC	1 cut(s)	373
BcoDI	GTCTC	3 cut(s)	138, 510, 596
BfaI	CTAG	3 cut(s)	83, 252, 344
BfuI	GTATCC	1 cut(s)	373
BisI	GCNGC	2 cut(s)	757, 844
BlnI	CCTAGG	1 cut(s)	251
BlsI	GCNGC	2 cut(s)	758, 845
Bme1390I	CCNGG	1 cut(s)	591
Bme18I	GGWCC	3 cut(s)	74, 86, 658
BmgT120I	GGNCC	3 cut(s)	74, 86, 658
BmiI	GGNNCC	1 cut(s)	75
BmrFI	CCNGG	1 cut(s)	591
BmsI	GCATC	2 cut(s)	41, 261
BpiI	GAAGAC	1 cut(s)	170
BpmI	CTGGAG	1 cut(s)	612
Bpu10I	CCTNAGC	1 cut(s)	504
Bpu14I	TTCGAA	1 cut(s)	161
Bsa29I	ATCGAT	1 cut(s)	57
BsaBI	GATNNNNATC	1 cut(s)	649
BsaJI	CCNNGG	2 cut(s)	251, 384
BsaWI	WCCGGW	1 cut(s)	551
Bsc4I	CCNNNNNNNGG	2 cut(s)	83, 655
Bse1I	ACTGG	1 cut(s)	660
Bse3DI	GCAATG	1 cut(s)	327
Bse8I	GATNNNNATC	1 cut(s)	649
BseBI	CCWGG	1 cut(s)	591
BseCI	ATCGAT	1 cut(s)	57
BseDI	CCNNGG	2 cut(s)	251, 384
BseGI	GGATG	2 cut(s)	107, 233
BseJI	GATNNNNATC	1 cut(s)	649
BseLI	CCNNNNNNNGG	2 cut(s)	83, 655
BseMI	GCAATG	1 cut(s)	327
BseMII	CTCAG	3 cut(s)	276, 411, 591
BseNI	ACTGG	1 cut(s)	660
BseRI	GAGGAG	1 cut(s)	191
BseXI	GCAGC	2 cut(s)	768, 830
BshVI	ATCGAT	1 cut(s)	57
BsiSI	CCGG	2 cut(s)	66, 552
BslFI	GGGAC	1 cut(s)	99
BslI	CCNNNNNNNGG	2 cut(s)	83, 655
BsmAI	GTCTC	3 cut(s)	138, 510, 596
BsmBI	CGTCTC	1 cut(s)	138
BsmFI	GGGAC	1 cut(s)	99
Bsp119I	TTCGAA	1 cut(s)	161
Bsp143I	GATC	4 cut(s)	247, 391, 435, 595
BspACI	CCGC	1 cut(s)	661
BspCNI	CTCAG	3 cut(s)	275, 412, 592
BspDI	ATCGAT	1 cut(s)	57
BspLI	GGNNCC	1 cut(s)	75
BspPI	GGATC	3 cut(s)	242, 386, 590
BspT104I	TTCGAA	1 cut(s)	161
BsrDI	GCAATG	1 cut(s)	327
BsrI	ACTGG	1 cut(s)	660
BssECI	CCNNGG	2 cut(s)	251, 384
BssMI	GATC	4 cut(s)	247, 391, 435, 595
BssT1I	CCWWGG	1 cut(s)	251
Bst2UI	CCWGG	1 cut(s)	591
Bst4CI	ACNGT	2 cut(s)	385, 467
Bst6I	CTCTTC	2 cut(s)	183, 729
BstBI	TTCGAA	1 cut(s)	161
BstC8I	GCNNGC	2 cut(s)	276, 862
BstDEI	CTNAG	6 cut(s)	262, 420, 504, 600, 776, 882
BstDSI	CCRYGG	1 cut(s)	384
BstENI	CCTNNNNNAGG	1 cut(s)	81
BstF5I	GGATG	2 cut(s)	107, 233
BstKTI	GATC	4 cut(s)	250, 394, 438, 598
BstMAI	GTCTC	3 cut(s)	138, 510, 596
BstMBI	GATC	4 cut(s)	247, 391, 435, 595
BstMWI	GCNNNNNNNGC	1 cut(s)	719
BstNI	CCWGG	1 cut(s)	591
BstNSI	RCATGY	1 cut(s)	801
BstSCI	CCNGG	1 cut(s)	589
BstV1I	GCAGC	2 cut(s)	768, 830
BstV2I	GAAGAC	1 cut(s)	170
BstX2I	RGATCY	2 cut(s)	391, 595
BstYI	RGATCY	2 cut(s)	391, 595
Bsu15I	ATCGAT	1 cut(s)	57
BsuI	GTATCC	1 cut(s)	373
BsuTUI	ATCGAT	1 cut(s)	57
BtgI	CCRYGG	1 cut(s)	384
BtsCI	GGATG	2 cut(s)	107, 233
BtsIMutI	CAGTG	1 cut(s)	653
Cac8I	GCNNGC	2 cut(s)	276, 862
Cfr13I	GGNCC	3 cut(s)	74, 86, 658
ClaI	ATCGAT	1 cut(s)	57
CseI	GACGC	1 cut(s)	849
Csp6I	GTAC	2 cut(s)	341, 403
CspCI	CAANNNNNGTGG	2 cut(s)	480, 515
CviAII	CATG	3 cut(s)	349, 714, 798
CviQI	GTAC	2 cut(s)	341, 403
DdeI	CTNAG	6 cut(s)	262, 420, 504, 600, 776, 882
DpnI	GATC	4 cut(s)	249, 393, 437, 597
DpnII	GATC	4 cut(s)	247, 391, 435, 595
DraI	TTTAAA	1 cut(s)	297
Eam1104I	CTCTTC	2 cut(s)	183, 729
EarI	CTCTTC	2 cut(s)	183, 729
Eco130I	CCWWGG	1 cut(s)	251
Eco32I	GATATC	1 cut(s)	587
Eco47I	GGWCC	3 cut(s)	74, 86, 658
Eco57I	CTGAAG	1 cut(s)	649
EcoNI	CCTNNNNNAGG	1 cut(s)	81
EcoO109I	RGGNCCY	2 cut(s)	74, 86
EcoRII	CCWGG	1 cut(s)	589
EcoRV	GATATC	1 cut(s)	587
EcoT14I	CCWWGG	1 cut(s)	251
ErhI	CCWWGG	1 cut(s)	251
Esp3I	CGTCTC	1 cut(s)	138
FaeI	CATG	3 cut(s)	352, 717, 801
FaqI	GGGAC	1 cut(s)	99
FatI	CATG	3 cut(s)	348, 713, 797
Fnu4HI	GCNGC	2 cut(s)	757, 844
FokI	GGATG	2 cut(s)	114, 240
Fsp4HI	GCNGC	2 cut(s)	757, 844
FspBI	CTAG	3 cut(s)	83, 252, 344
GluI	GCNGC	2 cut(s)	757, 844
GsuI	CTGGAG	1 cut(s)	612
HapII	CCGG	2 cut(s)	66, 552
HgaI	GACGC	1 cut(s)	849
Hin1II	CATG	3 cut(s)	352, 717, 801
HindIII	AAGCTT	2 cut(s)	202, 209
HinfI	GANTC	5 cut(s)	5, 158, 166, 645, 824
HpaII	CCGG	2 cut(s)	66, 552
HphI	GGTGA	1 cut(s)	520
Hpy188I	TCNGA	4 cut(s)	10, 363, 421, 477
Hpy188III	TCNNGA	4 cut(s)	377, 395, 582, 599
Hpy99I	CGWCG	1 cut(s)	449
HpyAV	CCTTC	3 cut(s)	63, 441, 688
HpyCH4III	ACNGT	2 cut(s)	385, 467
HpyCH4V	TGCA	6 cut(s)	155, 320, 622, 681, 713, 846
HpyF10VI	GCNNNNNNNGC	1 cut(s)	719
HpyF3I	CTNAG	6 cut(s)	262, 420, 504, 600, 776, 882
Hsp92II	CATG	3 cut(s)	352, 717, 801
Kzo9I	GATC	4 cut(s)	247, 391, 435, 595
Lsp1109I	GCAGC	2 cut(s)	768, 830
LweI	GCATC	2 cut(s)	41, 261
MaeI	CTAG	3 cut(s)	83, 252, 344
MaeIII	GTNAC	2 cut(s)	219, 508
MalI	GATC	4 cut(s)	249, 393, 437, 597
MboI	GATC	4 cut(s)	247, 391, 435, 595
MboII	GAAGA	6 cut(s)	175, 200, 350, 569, 746, 827
MflI	RGATCY	2 cut(s)	391, 595
MluCI	AATT	3 cut(s)	292, 413, 848
MlyI	GAGTC	2 cut(s)	160, 833
MnlI	CCTC	5 cut(s)	169, 361, 417, 538, 694
MseI	TTAA	2 cut(s)	206, 296
MslI	CAYNNNNRTG	2 cut(s)	25, 226
MspI	CCGG	2 cut(s)	66, 552
MspR9I	CCNGG	1 cut(s)	591
MvaI	CCWGG	1 cut(s)	591
MwoI	GCNNNNNNNGC	1 cut(s)	719
NdeII	GATC	4 cut(s)	247, 391, 435, 595
NlaIII	CATG	3 cut(s)	352, 717, 801
NlaIV	GGNNCC	1 cut(s)	75
NmuCI	GTSAC	2 cut(s)	219, 508
NspI	RCATGY	1 cut(s)	801
NspV	TTCGAA	1 cut(s)	161
PfeI	GAWTC	3 cut(s)	5, 158, 645
PfoI	TCCNGGA	1 cut(s)	589
PkrI	GCNGC	2 cut(s)	758, 845
PleI	GAGTC	2 cut(s)	160, 832
PpsI	GAGTC	2 cut(s)	160, 832
PpuMI	RGGWCCY	2 cut(s)	74, 86
Psp5II	RGGWCCY	2 cut(s)	74, 86
Psp6I	CCWGG	1 cut(s)	589
PspGI	CCWGG	1 cut(s)	589
PspN4I	GGNNCC	1 cut(s)	75
PspPI	GGNCC	3 cut(s)	74, 86, 658
PspPPI	RGGWCCY	2 cut(s)	74, 86
PsrI	GAACNNNNNNTAC	2 cut(s)	667, 699
PsuI	RGATCY	2 cut(s)	391, 595
RsaI	GTAC	2 cut(s)	342, 404
RsaNI	GTAC	2 cut(s)	341, 403
RseI	CAYNNNNRTG	2 cut(s)	25, 226
SaqAI	TTAA	2 cut(s)	206, 296
SatI	GCNGC	2 cut(s)	757, 844
Sau3AI	GATC	4 cut(s)	247, 391, 435, 595
Sau96I	GGNCC	3 cut(s)	74, 86, 658
SchI	GAGTC	2 cut(s)	160, 833
ScrFI	CCNGG	1 cut(s)	591
SetI	ASST	9 cut(s)	43, 88, 206, 213, 428, 463, 510, 705, 862
SfaNI	GCATC	2 cut(s)	41, 261
SfuI	TTCGAA	1 cut(s)	161
SinI	GGWCC	3 cut(s)	74, 86, 658
SmiI	ATTTAAAT	1 cut(s)	297
SmiMI	CAYNNNNRTG	2 cut(s)	25, 226
Sse9I	AATT	3 cut(s)	292, 413, 848
SsiI	CCGC	1 cut(s)	661
SspMI	CTAG	3 cut(s)	83, 252, 344
StyD4I	CCNGG	1 cut(s)	589
StyI	CCWWGG	1 cut(s)	251
SwaI	ATTTAAAT	1 cut(s)	297
TaaI	ACNGT	2 cut(s)	385, 467
TaqI	TCGA	5 cut(s)	57, 161, 434, 822, 827
TasI	AATT	3 cut(s)	292, 413, 848
TatI	WGTACW	1 cut(s)	402
TfiI	GAWTC	3 cut(s)	5, 158, 645
Tru1I	TTAA	2 cut(s)	206, 296
Tru9I	TTAA	2 cut(s)	206, 296
TscAI	CASTG	1 cut(s)	660
TseFI	GTSAC	2 cut(s)	219, 508
TseI	GCWGC	2 cut(s)	756, 843
Tsp45I	GTSAC	2 cut(s)	219, 508
TspDTI	ATGAA	4 cut(s)	75, 213, 741, 747
TspRI	CASTG	1 cut(s)	660
VpaK11BI	GGWCC	3 cut(s)	74, 86, 658
XagI	CCTNNNNNAGG	1 cut(s)	81
XapI	RAATTY	1 cut(s)	848
XceI	RCATGY	1 cut(s)	801
XmaJI	CCTAGG	1 cut(s)	251
XspI	CTAG	3 cut(s)	83, 252, 344

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.