Rh5CG365100

Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011443
Physical Location & Seq
Reverse (-)
44039786 .. 44042413
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG365100.1

Sequence Viewer

Length: 888 bp
ATGGGATCTGACACTACACTTCCCCAATGGGCGTCAGAGCCTTGTATCATGGGCATCGATGAAGCCGGCCGAGGCCCTGTTCTAGGGCCAATGGTGTATGGATGCTTATACTGTGCTCGCTCATATGAGAAGACTCTCTCCTCTTTGAACTTTGCGGATTCAAAGACACTAAAAGAAGAGAAGAGGGAGGAATTATTTGAGAATCTAAAGGCTGATGAATCAATTGGGTGGGCTGTCGATATCATAGATCCGAGAGAGCTTTCAGCTAAAATGCTAAAGAAGAATAAGATAAACCTTAATGAGATATCACATGACTCTGCAATTGGCCTTGTCACTAAGGTGCTTAACATGGGGGTTCTTCTAACTGAGGTCTACGTGGATACAGTGGGAGATACTGAAAAGTATAGAACTAAACTGTCTGAGAGATTTCCTGGAGTTAATTTTGTGGTTGCAAAGAAGGCTGATAGTCTTTACCCAGTTGTTAGTGGAGCAAGCATAGCTGCAAAAGTCACAAGGGACAGAGCCTTACGAGAGTGGGTGCTTGATGAAACAGCTGAAGATCTGCACAAAAACTTTGGTTCTGGATATCCTGGAGATCCCGATACCAAGGCCTGGTTGCAACATCACAAACACTCGGTATTTGGATTCCCAACATTGGTACGTTTTAGCTGGGGCACATGTACTCCATATTTTAAAGACATTGCTGAAGTCTTATGGGAATCAATGGATGACGATGGTTCTAGCAGTTCTAATGGAAAGCGGCAACTGAAATTAAGTAGTTTTGGTGTCACAACATCAAAGAGAAAGATTGAAGAAATAGAATCAAATGGTAAAGGACGCTGCAAATTTTTCCTGGCTCGTAAGCTTGAGCAAGTTACTCATTTCTAA

Protein Analysis

295

Amino Acids

32.99

Weight (kDa)

6.34

Isoelectric Point (pI)

28.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_HII PF01351 17 - 227 5.5e-52 Ribonuclease HII
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 612
AccI GTMKAC 1 cut(s) 372
AciI CCGC 2 cut(s) 155, 760
AclWI GGATC 3 cut(s) 13, 242, 590
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 1 cut(s) 845
AcuI CTGAAG 2 cut(s) 576, 726
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 660, 682
AfiI CCNNNNNNNGG 3 cut(s) 83, 612, 655
AflIII ACRYGT 1 cut(s) 677
AgsI TTSAA 3 cut(s) 148, 162, 812
AjnI CCWGG 4 cut(s) 430, 589, 611, 852
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 6 cut(s) 259, 266, 500, 554, 669, 865
AluI AGCT 6 cut(s) 259, 266, 500, 554, 669, 865
Alw21I GWGCWC 1 cut(s) 118
AlwI GGATC 3 cut(s) 13, 242, 590
AoxI GGCC 5 cut(s) 67, 73, 86, 325, 609
ApeKI GCWGC 2 cut(s) 500, 840
ApoI RAATTY 1 cut(s) 845
AspS9I GGNCC 2 cut(s) 74, 86
BaeGI GKGCMC 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 2 cut(s) 487, 827
BccI CCATC 1 cut(s) 728
BciT130I CCWGG 4 cut(s) 432, 591, 613, 854
BciVI GTATCC 1 cut(s) 373
BfaI CTAG 2 cut(s) 83, 741
BfuI GTATCC 1 cut(s) 373
BglII AGATCT 1 cut(s) 559
BisI GCNGC 3 cut(s) 501, 761, 841
BlsI GCNGC 3 cut(s) 502, 762, 842
Bme1390I CCNGG 4 cut(s) 432, 591, 613, 854
BmgT120I GGNCC 2 cut(s) 74, 86
BmrFI CCNGG 4 cut(s) 432, 591, 613, 854
BmrI ACTGGG 1 cut(s) 470
BmsI GCATC 2 cut(s) 63, 92
BmuI ACTGGG 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 137
BpmI CTGGAG 2 cut(s) 453, 612
BpuEI CTTGAG 1 cut(s) 887
Bsa29I ATCGAT 1 cut(s) 57
BsaAI YACGTR 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 70, 606
BsaXI ACNNNNNCTCC 2 cut(s) 667, 697
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 612, 655
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 4 cut(s) 432, 591, 613, 854
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 2 cut(s) 70, 606
BseGI GGATG 2 cut(s) 107, 733
BseLI CCNNNNNNNGG 3 cut(s) 83, 612, 655
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 2 cut(s) 357, 411
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 677
BseX3I CGGCCG 1 cut(s) 67
BseXI GCAGC 2 cut(s) 487, 827
BseYI CCCAGC 1 cut(s) 669
BsgI GTGCAG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 69, 75, 88, 327, 611
BshVI ATCGAT 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 3 cut(s) 83, 612, 655
BsmFI GGGAC 1 cut(s) 530
BsnI GGCC 5 cut(s) 69, 75, 88, 327, 611
Bsp1286I GDGCHC 2 cut(s) 118, 677
Bsp143I GATC 4 cut(s) 5, 247, 559, 595
BspACI CCGC 2 cut(s) 155, 760
BspANI GGCC 5 cut(s) 69, 75, 88, 327, 611
BspCNI CTCAG 2 cut(s) 358, 412
BspDI ATCGAT 1 cut(s) 57
BspPI GGATC 3 cut(s) 13, 242, 590
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 70, 606
BssMI GATC 4 cut(s) 5, 247, 559, 595
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 4 cut(s) 432, 591, 613, 854
Bst4CI ACNGT 3 cut(s) 113, 385, 417
Bst6I CTCTTC 2 cut(s) 171, 176
BstACI GRCGYC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 3 cut(s) 67, 118, 493
BstDEI CTNAG 3 cut(s) 336, 366, 420
BstENI CCTNNNNNAGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 107, 733
BstKTI GATC 4 cut(s) 8, 250, 562, 598
BstMBI GATC 4 cut(s) 5, 247, 559, 595
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 2 cut(s) 458, 497
BstNI CCWGG 4 cut(s) 432, 591, 613, 854
BstNSI RCATGY 1 cut(s) 681
BstSCI CCNGG 4 cut(s) 430, 589, 611, 852
BstSLI GKGCMC 1 cut(s) 677
BstV1I GCAGC 2 cut(s) 487, 827
BstV2I GAAGAC 1 cut(s) 137
BstX2I RGATCY 4 cut(s) 5, 247, 559, 595
BstYI RGATCY 4 cut(s) 5, 247, 559, 595
BstZI CGGCCG 1 cut(s) 67
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 5 cut(s) 69, 75, 88, 327, 611
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 2 cut(s) 107, 733
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 3 cut(s) 67, 118, 493
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 74, 86
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 2 cut(s) 21, 846
Csp6I GTAC 2 cut(s) 659, 681
CviAII CATG 4 cut(s) 49, 311, 349, 678
CviQI GTAC 2 cut(s) 659, 681
DdeI CTNAG 3 cut(s) 336, 366, 420
DpnI GATC 4 cut(s) 7, 249, 561, 597
DpnII GATC 4 cut(s) 5, 247, 559, 595
DraI TTTAAA 1 cut(s) 694
EaeI YGGCCR 1 cut(s) 67
EagI CGGCCG 1 cut(s) 67
Eam1104I CTCTTC 2 cut(s) 171, 176
EarI CTCTTC 2 cut(s) 171, 176
EclXI CGGCCG 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 606
Eco147I AGGCCT 1 cut(s) 611
Eco32I GATATC 3 cut(s) 241, 306, 587
Eco52I CGGCCG 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 576, 726
EcoNI CCTNNNNNAGG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 74
EcoRII CCWGG 4 cut(s) 430, 589, 611, 852
EcoRV GATATC 3 cut(s) 241, 306, 587
EcoT14I CCWWGG 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 4 cut(s) 52, 314, 352, 681
FaqI GGGAC 1 cut(s) 530
FatI CATG 4 cut(s) 48, 310, 348, 677
FauNDI CATATG 1 cut(s) 124
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 501, 761, 841
FokI GGATG 2 cut(s) 114, 740
Fsp4HI GCNGC 3 cut(s) 501, 761, 841
FspBI CTAG 2 cut(s) 83, 741
GluI GCNGC 3 cut(s) 501, 761, 841
GsaI CCCAGC 1 cut(s) 673
GsuI CTGGAG 2 cut(s) 453, 612
HaeIII GGCC 5 cut(s) 69, 75, 88, 327, 611
HapII CCGG 1 cut(s) 66
HgaI GACGC 2 cut(s) 21, 846
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 52, 314, 352, 681
HindIII AAGCTT 1 cut(s) 863
HinfI GANTC 8 cut(s) 133, 158, 202, 218, 314, 645, 719, 821
HpaII CCGG 1 cut(s) 66
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 4 cut(s) 10, 37, 252, 421
Hpy188III TCNNGA 2 cut(s) 582, 599
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 3 cut(s) 113, 385, 417
HpyCH4IV ACGT 2 cut(s) 375, 661
HpyCH4V TGCA 6 cut(s) 320, 452, 503, 565, 619, 843
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 497
HpyF3I CTNAG 3 cut(s) 336, 366, 420
HpySE526I ACGT 2 cut(s) 375, 661
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 52, 314, 352, 681
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 4 cut(s) 5, 247, 559, 595
LmnI GCTCC 1 cut(s) 488
Lsp1109I GCAGC 2 cut(s) 487, 827
LweI GCATC 2 cut(s) 63, 92
MaeI CTAG 2 cut(s) 83, 741
MaeII ACGT 2 cut(s) 375, 661
MaeIII GTNAC 4 cut(s) 331, 508, 787, 874
MalI GATC 4 cut(s) 7, 249, 561, 597
MboI GATC 4 cut(s) 5, 247, 559, 595
MboII GAAGA 7 cut(s) 142, 188, 193, 292, 350, 569, 824
MfeI CAATTG 2 cut(s) 222, 321
MflI RGATCY 4 cut(s) 5, 247, 559, 595
MhlI GDGCHC 2 cut(s) 118, 677
MluCI AATT 6 cut(s) 191, 222, 321, 439, 770, 845
MlyI GAGTC 2 cut(s) 127, 308
MnlI CCTC 5 cut(s) 65, 151, 177, 181, 361
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 5 cut(s) 297, 345, 438, 693, 773
MslI CAYNNNNRTG 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 4 cut(s) 432, 591, 613, 854
MunI CAATTG 2 cut(s) 222, 321
MvaI CCWGG 4 cut(s) 432, 591, 613, 854
MwoI GCNNNNNNNGC 2 cut(s) 458, 497
NaeI GCCGGC 1 cut(s) 67
NdeI CATATG 1 cut(s) 124
NdeII GATC 4 cut(s) 5, 247, 559, 595
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 4 cut(s) 52, 314, 352, 681
NmeAIII GCCGAG 1 cut(s) 95
NmuCI GTSAC 3 cut(s) 331, 508, 787
NspI RCATGY 1 cut(s) 681
OliI CACNNNNGTG 1 cut(s) 338
PceI AGGCCT 1 cut(s) 611
PciI ACATGT 1 cut(s) 677
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 6 cut(s) 158, 202, 218, 645, 719, 821
PflMI CCANNNNNTGG 1 cut(s) 612
PfoI TCCNGGA 2 cut(s) 430, 589
PkrI GCNGC 3 cut(s) 502, 762, 842
PleI GAGTC 2 cut(s) 127, 308
PpsI GAGTC 2 cut(s) 127, 308
Ppu21I YACGTR 1 cut(s) 376
PscI ACATGT 1 cut(s) 677
Psp6I CCWGG 4 cut(s) 430, 589, 611, 852
PspFI CCCAGC 1 cut(s) 669
PspGI CCWGG 4 cut(s) 430, 589, 611, 852
PspPI GGNCC 2 cut(s) 74, 86
PsuI RGATCY 4 cut(s) 5, 247, 559, 595
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 2 cut(s) 660, 682
RsaNI GTAC 2 cut(s) 659, 681
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 5 cut(s) 297, 345, 438, 693, 773
SatI GCNGC 3 cut(s) 501, 761, 841
Sau3AI GATC 4 cut(s) 5, 247, 559, 595
Sau96I GGNCC 2 cut(s) 74, 86
SchI GAGTC 2 cut(s) 127, 308
ScrFI CCNGG 4 cut(s) 432, 591, 613, 854
SduI GDGCHC 2 cut(s) 118, 677
SfaNI GCATC 2 cut(s) 63, 92
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 1 cut(s) 866
SmoI CTYRAG 1 cut(s) 866
Sse9I AATT 6 cut(s) 191, 222, 321, 439, 770, 845
SseBI AGGCCT 1 cut(s) 611
SsiI CCGC 2 cut(s) 155, 760
SspMI CTAG 2 cut(s) 83, 741
StuI AGGCCT 1 cut(s) 611
StyD4I CCNGG 4 cut(s) 430, 589, 611, 852
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 3 cut(s) 113, 385, 417
TaiI ACGT 2 cut(s) 378, 664
TaqI TCGA 2 cut(s) 57, 237
TasI AATT 6 cut(s) 191, 222, 321, 439, 770, 845
TatI WGTACW 1 cut(s) 680
TauI GCSGC 1 cut(s) 763
TfiI GAWTC 6 cut(s) 158, 202, 218, 645, 719, 821
Tru1I TTAA 5 cut(s) 297, 345, 438, 693, 773
Tru9I TTAA 5 cut(s) 297, 345, 438, 693, 773
TscAI CASTG 1 cut(s) 390
TseFI GTSAC 3 cut(s) 331, 508, 787
TseI GCWGC 2 cut(s) 500, 840
Tsp45I GTSAC 3 cut(s) 331, 508, 787
TspDTI ATGAA 3 cut(s) 75, 231, 561
TspRI CASTG 1 cut(s) 390
Van91I CCANNNNNTGG 1 cut(s) 612
XagI CCTNNNNNAGG 1 cut(s) 81
XapI RAATTY 1 cut(s) 845
XceI RCATGY 1 cut(s) 681
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 2 cut(s) 83, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.