Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011443

Physical Location & Seq

Genomic Coordinates Forward (+)

31603370 .. 31606935

Gene Structure 3566 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1261600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 900 bp

ATGGGATCGGAGCGTGAAGCTGCAATCCCCGAATGGGCGTCAGAGCCTTGCATCATGGGCATCGATGAAGCTGGCCGTGGCCCTGTTTTAGGTCCAATGGTGTACGGATGCTTGTACTGTGCCCGCTCGTACCAGAATACTCTCTCCTCTTTGAACTTCGCAGATTCAAAGACATTGAAGGAAGAGAAGAGGGAGGAATTGTTTGAGAATTTGAAGGCTGATGAATCCATTGGGTGGGCTGTTGATGTCATAGATCCAAGGGAACTTTCGGCCAAAATGCTAAAGAAACGTAAGATAAACCTTAATGAAATATCACATGACTCTGCAATGGGTCTGGTCACAAAGGTCCTAAACATGGGAGTTCTTCTAACCGAGGTTTATGTGGATACAGTGGGTGATGCTGAGAAGTATAGAGCTAAACTGTCTGATAGATTCCCAGCTGTCAAGTTTGTGGTTGCGAAGAAGGCTGATAGCCTATATCCTGTTGTCAGTGGAGCAAGCATAGTTGCAAAAGTTACAAGAGACAGAGCGTTGCGTGAGTGGGTGCTCGAGGAAACAGCTGAAAACTTGCATAAAGACTTTGGTTCTGGATATCCTGGAGATCCGAATACAAAGGCATGGTTGGAACATCACAAGCACTTCGTTTTTGGATTCCCATCGTTGGTCCGTTTCAGCTGGGGTACATGCACTCCTTATTTCAAAGACATTGCGGAAGTCTTATGGGAATCCGATGAAATGGATGAAGATGGTTCTAGCACTGCTAATGGCAAGCGGCAAATGAAACTAAGTAGTTTTGGTGTTACAACATCCAAGAGAAAGATTGAAGAAATTGAATCAAGTGGTAAAGGGCGCTGCAAATTTTTTCTTGCTCGTAAACTTGAGCAAGTTACTCAATTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004523 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006273 GO:0006281 GO:0006298 GO:0006401 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019439 GO:0022616 GO:0032299 GO:0032991 GO:0033554 GO:0033567 GO:0034641 GO:0034645 GO:0034655 GO:0043137 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0046483 GO:0046700 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 map03030

Pfam Domains

Protein Families

RNase_HII

Protein Analysis

300

Amino Acids

33.54

Weight (kDa)

6.06

Isoelectric Point (pI)

28.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNase_HII	PF01351	19 - 229	4.7e-53	Ribonuclease HII

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25100 AT2G25100
fragaria_vesca	FvH4_3g27560 FvH4_3g27560
malus_domestica	MD02G1261600.v1.1
prunus_persica	Prupe.2G069600_v2.0.a1 Prupe.2G069600_v2.0.a1
pyrus_communis	pycom02g22370
rosa_chinensis	RchiOBHm_Chr5g0049931
rosa_laevigata	RLG00000034666
rosa_roxburghii	Rroxscaffold_1G00031380
rosa_rugosa	Rorug05G0254300 Rorug05G0254400 Rorug05G0254500 Rorug05G0254600
rosa_samantha	Rh5AG328700 Rh5BG339300 Rh5CG365100 Rh5DG351700
rosa_wichuraiana	Rw5G031050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	234
AccBSI	CCGCTC	1 cut(s)	126
AciI	CCGC	3 cut(s)	124, 710, 772
AclWI	GGATC	3 cut(s)	13, 248, 596
AcoI	YGGCCR	2 cut(s)	73, 270
AcsI	RAATTY	2 cut(s)	208, 857
AcyI	GRCGYC	1 cut(s)	38
AfaI	GTAC	4 cut(s)	104, 116, 131, 682
AfiI	CCNNNNNNNGG	5 cut(s)	34, 89, 234, 355, 661
AgsI	TTSAA	7 cut(s)	154, 168, 178, 214, 700, 824, 833
AjnI	CCWGG	1 cut(s)	595
AjuI	GAANNNNNNNTTGG	2 cut(s)	250, 282
AluBI	AGCT	6 cut(s)	20, 71, 416, 440, 560, 675
AluI	AGCT	6 cut(s)	20, 71, 416, 440, 560, 675
Alw21I	GWGCWC	1 cut(s)	549
Alw26I	GTCTC	1 cut(s)	516
AlwI	GGATC	3 cut(s)	13, 248, 596
Ama87I	CYCGRG	1 cut(s)	548
AoxI	GGCC	3 cut(s)	73, 79, 270
ApeKI	GCWGC	2 cut(s)	20, 852
ApoI	RAATTY	2 cut(s)	208, 857
AspLEI	GCGC	1 cut(s)	852
AspS9I	GGNCC	4 cut(s)	80, 92, 346, 664
AsuHPI	GGTGA	1 cut(s)	407
AvaI	CYCGRG	1 cut(s)	548
AvaII	GGWCC	3 cut(s)	92, 346, 664
BaeGI	GKGCMC	1 cut(s)	124
Bbv12I	GWGCWC	1 cut(s)	549
BbvI	GCAGC	2 cut(s)	7, 839
BccI	CCATC	2 cut(s)	664, 740
BceAI	ACGGC	1 cut(s)	60
BciT130I	CCWGG	1 cut(s)	597
BciVI	GTATCC	1 cut(s)	379
BcoDI	GTCTC	1 cut(s)	516
BfaI	CTAG	1 cut(s)	753
BfoI	RGCGCY	1 cut(s)	853
BfuI	GTATCC	1 cut(s)	379
BisI	GCNGC	3 cut(s)	21, 773, 853
BlsI	GCNGC	3 cut(s)	22, 774, 854
Bme1390I	CCNGG	1 cut(s)	597
Bme18I	GGWCC	3 cut(s)	92, 346, 664
BmeT110I	CYCGRG	1 cut(s)	548
BmgT120I	GGNCC	4 cut(s)	80, 92, 346, 664
BmrFI	CCNGG	1 cut(s)	597
BmsI	GCATC	4 cut(s)	60, 69, 98, 388
BpmI	CTGGAG	1 cut(s)	618
BpuEI	CTTGAG	1 cut(s)	899
Bsa29I	ATCGAT	1 cut(s)	63
BsaBI	GATNNNNATC	1 cut(s)	655
BsaHI	GRCGYC	1 cut(s)	38
BsaJI	CCNNGG	3 cut(s)	76, 257, 372
BsaXI	ACNNNNNCTCC	4 cut(s)	185, 215, 673, 703
Bsc4I	CCNNNNNNNGG	5 cut(s)	34, 89, 234, 355, 661
Bse3DI	GCAATG	2 cut(s)	333, 705
Bse8I	GATNNNNATC	1 cut(s)	655
BseBI	CCWGG	1 cut(s)	597
BseCI	ATCGAT	1 cut(s)	63
BseDI	CCNNGG	3 cut(s)	76, 257, 372
BseGI	GGATG	3 cut(s)	113, 745, 806
BseJI	GATNNNNATC	1 cut(s)	655
BseLI	CCNNNNNNNGG	5 cut(s)	34, 89, 234, 355, 661
BseMI	GCAATG	2 cut(s)	333, 705
BseMII	CTCAG	1 cut(s)	393
BseRI	GAGGAG	1 cut(s)	136
BseSI	GKGCMC	1 cut(s)	124
BseXI	GCAGC	2 cut(s)	7, 839
BseYI	CCCAGC	2 cut(s)	436, 675
BshFI	GGCC	3 cut(s)	75, 81, 272
BshVI	ATCGAT	1 cut(s)	63
BsiHKAI	GWGCWC	1 cut(s)	549
BsiHKCI	CYCGRG	1 cut(s)	548
BslI	CCNNNNNNNGG	5 cut(s)	34, 89, 234, 355, 661
BsmAI	GTCTC	1 cut(s)	516
BsnI	GGCC	3 cut(s)	75, 81, 272
BsoBI	CYCGRG	1 cut(s)	548
Bsp1286I	GDGCHC	2 cut(s)	124, 549
Bsp143I	GATC	3 cut(s)	5, 253, 601
BspACI	CCGC	3 cut(s)	124, 710, 772
BspANI	GGCC	3 cut(s)	75, 81, 272
BspCNI	CTCAG	1 cut(s)	394
BspDI	ATCGAT	1 cut(s)	63
BspPI	GGATC	3 cut(s)	13, 248, 596
BsrBI	CCGCTC	1 cut(s)	126
BsrDI	GCAATG	2 cut(s)	333, 705
BssECI	CCNNGG	3 cut(s)	76, 257, 372
BssMI	GATC	3 cut(s)	5, 253, 601
BssNI	GRCGYC	1 cut(s)	38
BssT1I	CCWWGG	1 cut(s)	257
Bst2UI	CCWGG	1 cut(s)	597
Bst4CI	ACNGT	3 cut(s)	119, 391, 423
Bst6I	CTCTTC	2 cut(s)	177, 182
BstACI	GRCGYC	1 cut(s)	38
BstC8I	GCNNGC	4 cut(s)	73, 124, 499, 770
BstDEI	CTNAG	2 cut(s)	402, 785
BstDSI	CCRYGG	1 cut(s)	76
BstENI	CCTNNNNNAGG	1 cut(s)	87
BstF5I	GGATG	3 cut(s)	113, 745, 806
BstH2I	RGCGCY	1 cut(s)	853
BstHHI	GCGC	1 cut(s)	852
BstKTI	GATC	3 cut(s)	8, 256, 604
BstMAI	GTCTC	1 cut(s)	516
BstMBI	GATC	3 cut(s)	5, 253, 601
BstMWI	GCNNNNNNNGC	2 cut(s)	57, 464
BstNI	CCWGG	1 cut(s)	597
BstNSI	RCATGY	1 cut(s)	687
BstSCI	CCNGG	1 cut(s)	595
BstSLI	GKGCMC	1 cut(s)	124
BstV1I	GCAGC	2 cut(s)	7, 839
BstX2I	RGATCY	2 cut(s)	253, 601
BstYI	RGATCY	2 cut(s)	253, 601
Bsu15I	ATCGAT	1 cut(s)	63
BsuI	GTATCC	1 cut(s)	379
BsuRI	GGCC	3 cut(s)	75, 81, 272
BsuTUI	ATCGAT	1 cut(s)	63
BtgI	CCRYGG	1 cut(s)	76
BtsCI	GGATG	3 cut(s)	113, 745, 806
BtsI	GCAGTG	1 cut(s)	756
BtsIMutI	CAGTG	3 cut(s)	396, 496, 756
Cac8I	GCNNGC	4 cut(s)	73, 124, 499, 770
CfoI	GCGC	1 cut(s)	852
Cfr13I	GGNCC	4 cut(s)	80, 92, 346, 664
ClaI	ATCGAT	1 cut(s)	63
CseI	GACGC	1 cut(s)	27
Csp6I	GTAC	4 cut(s)	103, 115, 130, 681
CviAII	CATG	5 cut(s)	55, 317, 355, 618, 684
CviQI	GTAC	4 cut(s)	103, 115, 130, 681
DdeI	CTNAG	2 cut(s)	402, 785
DpnI	GATC	3 cut(s)	7, 255, 603
DpnII	GATC	3 cut(s)	5, 253, 601
EaeI	YGGCCR	2 cut(s)	73, 270
Eam1104I	CTCTTC	2 cut(s)	177, 182
EarI	CTCTTC	2 cut(s)	177, 182
Eco130I	CCWWGG	1 cut(s)	257
Eco32I	GATATC	1 cut(s)	593
Eco47I	GGWCC	3 cut(s)	92, 346, 664
Eco88I	CYCGRG	1 cut(s)	548
EcoNI	CCTNNNNNAGG	1 cut(s)	87
EcoO109I	RGGNCCY	1 cut(s)	346
EcoRII	CCWGG	1 cut(s)	595
EcoRV	GATATC	1 cut(s)	593
EcoT14I	CCWWGG	1 cut(s)	257
ErhI	CCWWGG	1 cut(s)	257
FaeI	CATG	5 cut(s)	58, 320, 358, 621, 687
FatI	CATG	5 cut(s)	54, 316, 354, 617, 683
FauI	CCCGC	1 cut(s)	131
Fnu4HI	GCNGC	3 cut(s)	21, 773, 853
FokI	GGATG	3 cut(s)	120, 752, 793
Fsp4HI	GCNGC	3 cut(s)	21, 773, 853
FspBI	CTAG	1 cut(s)	753
GlaI	GCGC	1 cut(s)	851
GluI	GCNGC	3 cut(s)	21, 773, 853
GsaI	CCCAGC	2 cut(s)	440, 679
GsuI	CTGGAG	1 cut(s)	618
HaeII	RGCGCY	1 cut(s)	853
HaeIII	GGCC	3 cut(s)	75, 81, 272
HgaI	GACGC	1 cut(s)	27
HhaI	GCGC	1 cut(s)	852
Hin1I	GRCGYC	1 cut(s)	38
Hin1II	CATG	5 cut(s)	58, 320, 358, 621, 687
Hin6I	GCGC	1 cut(s)	850
HinP1I	GCGC	1 cut(s)	850
HinfI	GANTC	7 cut(s)	164, 224, 320, 432, 651, 725, 833
HphI	GGTGA	1 cut(s)	407
Hpy166II	GTNNAC	2 cut(s)	103, 875
Hpy188I	TCNGA	5 cut(s)	10, 43, 427, 606, 730
Hpy188III	TCNNGA	1 cut(s)	588
Hpy8I	GTNNAC	2 cut(s)	103, 875
HpyAV	CCTTC	3 cut(s)	172, 208, 457
HpyCH4III	ACNGT	3 cut(s)	119, 391, 423
HpyCH4IV	ACGT	1 cut(s)	289
HpyCH4V	TGCA	7 cut(s)	23, 51, 326, 509, 571, 687, 855
HpyF10VI	GCNNNNNNNGC	2 cut(s)	57, 464
HpyF3I	CTNAG	2 cut(s)	402, 785
HpySE526I	ACGT	1 cut(s)	289
Hsp92I	GRCGYC	1 cut(s)	38
Hsp92II	CATG	5 cut(s)	58, 320, 358, 621, 687
HspAI	GCGC	1 cut(s)	850
Kzo9I	GATC	3 cut(s)	5, 253, 601
LmnI	GCTCC	2 cut(s)	10, 494
Lsp1109I	GCAGC	2 cut(s)	7, 839
LweI	GCATC	4 cut(s)	60, 69, 98, 388
MaeI	CTAG	1 cut(s)	753
MaeII	ACGT	1 cut(s)	289
MaeIII	GTNAC	4 cut(s)	337, 514, 799, 886
MalI	GATC	3 cut(s)	7, 255, 603
MbiI	CCGCTC	1 cut(s)	126
MboI	GATC	3 cut(s)	5, 253, 601
MboII	GAAGA	6 cut(s)	194, 199, 356, 472, 755, 836
MflI	RGATCY	2 cut(s)	253, 601
MhlI	GDGCHC	2 cut(s)	124, 549
MluCI	AATT	5 cut(s)	197, 208, 828, 857, 893
MlyI	GAGTC	1 cut(s)	314
MmeI	TCCRAC	1 cut(s)	603
MnlI	CCTC	5 cut(s)	157, 183, 187, 367, 544
MseI	TTAA	1 cut(s)	303
MspA1I	CMGCKG	3 cut(s)	440, 560, 675
MspR9I	CCNGG	1 cut(s)	597
MvaI	CCWGG	1 cut(s)	597
MwoI	GCNNNNNNNGC	2 cut(s)	57, 464
NdeII	GATC	3 cut(s)	5, 253, 601
NlaIII	CATG	5 cut(s)	58, 320, 358, 621, 687
NmuCI	GTSAC	1 cut(s)	337
NspI	RCATGY	1 cut(s)	687
PaeR7I	CTCGAG	1 cut(s)	548
PfeI	GAWTC	6 cut(s)	164, 224, 432, 651, 725, 833
PflMI	CCANNNNNTGG	1 cut(s)	234
PfoI	TCCNGGA	1 cut(s)	595
PkrI	GCNGC	3 cut(s)	22, 774, 854
PleI	GAGTC	1 cut(s)	314
PpsI	GAGTC	1 cut(s)	314
PpuMI	RGGWCCY	1 cut(s)	346
Psp5II	RGGWCCY	1 cut(s)	346
Psp6I	CCWGG	1 cut(s)	595
PspFI	CCCAGC	2 cut(s)	436, 675
PspGI	CCWGG	1 cut(s)	595
PspPI	GGNCC	4 cut(s)	80, 92, 346, 664
PspPPI	RGGWCCY	1 cut(s)	346
PspXI	VCTCGAGB	1 cut(s)	548
PsuI	RGATCY	2 cut(s)	253, 601
PvuII	CAGCTG	3 cut(s)	440, 560, 675
RsaI	GTAC	4 cut(s)	104, 116, 131, 682
RsaNI	GTAC	4 cut(s)	103, 115, 130, 681
SaqAI	TTAA	1 cut(s)	303
SatI	GCNGC	3 cut(s)	21, 773, 853
Sau3AI	GATC	3 cut(s)	5, 253, 601
Sau96I	GGNCC	4 cut(s)	80, 92, 346, 664
SchI	GAGTC	1 cut(s)	314
ScrFI	CCNGG	1 cut(s)	597
SduI	GDGCHC	2 cut(s)	124, 549
SfaNI	GCATC	4 cut(s)	60, 69, 98, 388
Sfr274I	CTCGAG	1 cut(s)	548
SinI	GGWCC	3 cut(s)	92, 346, 664
SlaI	CTCGAG	1 cut(s)	548
SmlI	CTYRAG	2 cut(s)	548, 878
SmoI	CTYRAG	2 cut(s)	548, 878
Sse9I	AATT	5 cut(s)	197, 208, 828, 857, 893
SsiI	CCGC	3 cut(s)	124, 710, 772
SspMI	CTAG	1 cut(s)	753
StyD4I	CCNGG	1 cut(s)	595
StyI	CCWWGG	1 cut(s)	257
TaaI	ACNGT	3 cut(s)	119, 391, 423
TaiI	ACGT	1 cut(s)	292
TaqI	TCGA	2 cut(s)	63, 549
TasI	AATT	5 cut(s)	197, 208, 828, 857, 893
TatI	WGTACW	1 cut(s)	114
TauI	GCSGC	1 cut(s)	775
TfiI	GAWTC	6 cut(s)	164, 224, 432, 651, 725, 833
Tru1I	TTAA	1 cut(s)	303
Tru9I	TTAA	1 cut(s)	303
TscAI	CASTG	3 cut(s)	396, 496, 763
TseFI	GTSAC	1 cut(s)	337
TseI	GCWGC	2 cut(s)	20, 852
Tsp45I	GTSAC	1 cut(s)	337
TspDTI	ATGAA	6 cut(s)	81, 237, 321, 747, 756, 794
TspGWI	ACGGA	2 cut(s)	120, 656
TspRI	CASTG	3 cut(s)	396, 496, 763
Van91I	CCANNNNNTGG	1 cut(s)	234
VpaK11BI	GGWCC	3 cut(s)	92, 346, 664
XagI	CCTNNNNNAGG	1 cut(s)	87
XapI	RAATTY	2 cut(s)	208, 857
XceI	RCATGY	1 cut(s)	687
XhoI	CTCGAG	1 cut(s)	548
XspI	CTAG	1 cut(s)	753

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.