FvH4_3g27560

Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011443
Physical Location & Seq
Reverse (-)
20616803 .. 20619757
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27560.t2

Sequence Viewer

Length: 888 bp
ATGGGATCTGGCACTACACTTCCCCAATGGACGTCAGAGCCTTGTATCATGGGCATTGATGAAGCCGGTCGAGGCCCTGTTTTAGGGCCAATGGTATATGGATGCTTATACTGTGCTCGCTCATACGAGAAGACTCTCTCCTCTTTGAACTTTGCAGATTCAAAGACATTAAAAGAAGAGAAGAGGGAGGAATCATTTGAGAATCTAAAGACTGATGAATCAATTGGCTGGGCTGTTGATATCATAGATCCGAGAGAGCTTTCAGCTAAAATGCTAAAGAAGATTAAGATAAACCTTAATGAAATATCACATGACTCTGCAATTGGCCTTGTCACTAAGGTACTTAACATGGGGGTTCTTCTTGCTGAGGTTTACGTGGATACAGTGGGAGATACTGAAAAGTATAGGAGTAAACTGTCTGAAAGATTTCCTTGTATCAATTTTGTGGTTGCAAAGAAGGCTGATAGTCTTTACCCAGTTGTTAGTGGAGCAAGCATAGCTGCAAAAGTCACAAGGGACAGAGCCTTACGGGAGTGGGTTTTTGATGAAACAGCTGGAGCTCTGCACAAGAACTTTGGTTCTGGATATCCTGGAGATCCCGTTACCACGGCCTGGTTGCAAAATCACAAACACTCGGTATTTGGATTCCCATCATTGGTGCGTTTCAGCTGGGGCACATGTACTCCATATTTCAAAGACATTGCTGAAGTCTTATGGGAATCAGTGGATGACGATGGTTCTAGCAATTCTAACGGAAAGCGGCAACTGAAATTAAGTAATTTTGGTGTCACAACATCAAAGAAAAAGATTGAAGAAATAGAATCCAGTGGTAAAGGACGCTGCAAATTTTTCTTCGCTCGTAAGTTTGAGCAAGTCACTCATTTCTAA

Protein Analysis

296

Amino Acids

32.85

Weight (kDa)

7.58

Isoelectric Point (pI)

27.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_HII PF01351 17 - 227 3.5e-49 Ribonuclease HII
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 612
AciI CCGC 1 cut(s) 760
AclWI GGATC 3 cut(s) 13, 242, 590
AcsI RAATTY 1 cut(s) 845
AcuI CTGAAG 1 cut(s) 726
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 342, 682
AfiI CCNNNNNNNGG 3 cut(s) 83, 612, 655
AflIII ACRYGT 1 cut(s) 677
AgsI TTSAA 4 cut(s) 148, 162, 694, 812
AjnI CCWGG 2 cut(s) 589, 611
AluBI AGCT 6 cut(s) 259, 266, 500, 554, 560, 669
AluI AGCT 6 cut(s) 259, 266, 500, 554, 560, 669
Alw21I GWGCWC 2 cut(s) 118, 562
AlwI GGATC 3 cut(s) 13, 242, 590
AoxI GGCC 4 cut(s) 73, 86, 325, 609
ApeKI GCWGC 2 cut(s) 500, 840
ApoI RAATTY 1 cut(s) 845
Asp700I GAANNNNTTC 1 cut(s) 426
AspS9I GGNCC 2 cut(s) 74, 86
BaeGI GKGCMC 1 cut(s) 677
BanII GRGCYC 1 cut(s) 562
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 2 cut(s) 118, 562
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 487, 827
BccI CCATC 2 cut(s) 658, 728
BceAI ACGGC 1 cut(s) 624
BciT130I CCWGG 2 cut(s) 591, 613
BciVI GTATCC 1 cut(s) 373
BfaI CTAG 1 cut(s) 741
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 3 cut(s) 501, 761, 841
BlsI GCNGC 3 cut(s) 502, 762, 842
Bme1390I CCNGG 2 cut(s) 591, 613
BmgT120I GGNCC 2 cut(s) 74, 86
BmrFI CCNGG 2 cut(s) 591, 613
BmrI ACTGGG 1 cut(s) 470
BmsI GCATC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 137
BpmI CTGGAG 2 cut(s) 576, 612
Bpu10I CCTNAGC 1 cut(s) 366
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 1 cut(s) 649
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 606
BsaXI ACNNNNNCTCC 4 cut(s) 585, 615, 667, 697
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 612, 655
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 476, 825
Bse3DI GCAATG 1 cut(s) 699
Bse8I GATNNNNATC 1 cut(s) 649
BseBI CCWGG 2 cut(s) 591, 613
BseDI CCNNGG 1 cut(s) 606
BseGI GGATG 2 cut(s) 107, 733
BseJI GATNNNNATC 1 cut(s) 649
BseLI CCNNNNNNNGG 3 cut(s) 83, 612, 655
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 476, 825
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 2 cut(s) 487, 827
BseYI CCCAGC 2 cut(s) 228, 669
BsgI GTGCAG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 4 cut(s) 75, 88, 327, 611
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 2 cut(s) 118, 562
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 3 cut(s) 83, 612, 655
BsmFI GGGAC 1 cut(s) 530
BsnI GGCC 4 cut(s) 75, 88, 327, 611
Bsp1286I GDGCHC 3 cut(s) 118, 562, 677
Bsp143I GATC 3 cut(s) 5, 247, 595
BspACI CCGC 1 cut(s) 760
BspANI GGCC 4 cut(s) 75, 88, 327, 611
BspCNI CTCAG 1 cut(s) 358
BspPI GGATC 3 cut(s) 13, 242, 590
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 2 cut(s) 476, 825
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 606
BssMI GATC 3 cut(s) 5, 247, 595
BssNI GRCGYC 1 cut(s) 32
Bst2UI CCWGG 2 cut(s) 591, 613
Bst4CI ACNGT 3 cut(s) 113, 385, 417
Bst6I CTCTTC 2 cut(s) 171, 176
BstACI GRCGYC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 118, 493
BstDEI CTNAG 2 cut(s) 336, 366
BstDSI CCRYGG 1 cut(s) 606
BstENI CCTNNNNNAGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 107, 733
BstKTI GATC 3 cut(s) 8, 250, 598
BstMBI GATC 3 cut(s) 5, 247, 595
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 2 cut(s) 458, 497
BstNI CCWGG 2 cut(s) 591, 613
BstNSI RCATGY 1 cut(s) 681
BstSCI CCNGG 2 cut(s) 589, 611
BstSLI GKGCMC 1 cut(s) 677
BstV1I GCAGC 2 cut(s) 487, 827
BstV2I GAAGAC 1 cut(s) 137
BstX2I RGATCY 3 cut(s) 5, 247, 595
BstYI RGATCY 3 cut(s) 5, 247, 595
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 4 cut(s) 75, 88, 327, 611
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 2 cut(s) 107, 733
BtsIMutI CAGTG 3 cut(s) 390, 729, 832
Cac8I GCNNGC 2 cut(s) 118, 493
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 74, 86
CseI GACGC 1 cut(s) 846
Csp6I GTAC 2 cut(s) 341, 681
CviAII CATG 4 cut(s) 49, 311, 349, 678
CviQI GTAC 2 cut(s) 341, 681
DdeI CTNAG 2 cut(s) 336, 366
DpnI GATC 3 cut(s) 7, 249, 597
DpnII GATC 3 cut(s) 5, 247, 595
Eam1104I CTCTTC 2 cut(s) 171, 176
EarI CTCTTC 2 cut(s) 171, 176
Ecl136II GAGCTC 1 cut(s) 560
Eco24I GRGCYC 1 cut(s) 562
Eco32I GATATC 2 cut(s) 241, 587
Eco53kI GAGCTC 1 cut(s) 560
Eco57I CTGAAG 1 cut(s) 726
EcoICRI GAGCTC 1 cut(s) 560
EcoNI CCTNNNNNAGG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 589, 611
EcoRV GATATC 2 cut(s) 241, 587
EcoT38I GRGCYC 1 cut(s) 562
FaeI CATG 4 cut(s) 52, 314, 352, 681
FalI AAGNNNNNCTT 2 cut(s) 415, 447
FaqI GGGAC 1 cut(s) 530
FatI CATG 4 cut(s) 48, 310, 348, 677
Fnu4HI GCNGC 3 cut(s) 501, 761, 841
FokI GGATG 2 cut(s) 114, 740
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 3 cut(s) 501, 761, 841
FspBI CTAG 1 cut(s) 741
GluI GCNGC 3 cut(s) 501, 761, 841
GsaI CCCAGC 2 cut(s) 232, 673
GsuI CTGGAG 2 cut(s) 576, 612
HaeIII GGCC 4 cut(s) 75, 88, 327, 611
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 846
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 52, 314, 352, 681
HinfI GANTC 9 cut(s) 133, 158, 191, 202, 218, 314, 645, 719, 821
HpaII CCGG 1 cut(s) 66
Hpy166II GTNNAC 2 cut(s) 373, 413
Hpy188I TCNGA 3 cut(s) 37, 252, 421
Hpy188III TCNNGA 1 cut(s) 582
Hpy8I GTNNAC 2 cut(s) 373, 413
HpyAV CCTTC 1 cut(s) 451
HpyCH4III ACNGT 3 cut(s) 113, 385, 417
HpyCH4IV ACGT 2 cut(s) 32, 375
HpyCH4V TGCA 7 cut(s) 155, 320, 452, 503, 565, 619, 843
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 497
HpyF3I CTNAG 2 cut(s) 336, 366
HpySE526I ACGT 2 cut(s) 32, 375
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 52, 314, 352, 681
Kzo9I GATC 3 cut(s) 5, 247, 595
LmnI GCTCC 2 cut(s) 488, 557
Lsp1109I GCAGC 2 cut(s) 487, 827
LweI GCATC 1 cut(s) 92
MaeI CTAG 1 cut(s) 741
MaeII ACGT 2 cut(s) 32, 375
MaeIII GTNAC 5 cut(s) 331, 508, 601, 787, 874
MalI GATC 3 cut(s) 7, 249, 597
MboI GATC 3 cut(s) 5, 247, 595
MboII GAAGA 7 cut(s) 142, 188, 193, 292, 350, 824, 844
MfeI CAATTG 2 cut(s) 222, 321
MflI RGATCY 3 cut(s) 5, 247, 595
MhlI GDGCHC 3 cut(s) 118, 562, 677
MluCI AATT 7 cut(s) 222, 321, 439, 745, 770, 778, 845
MlyI GAGTC 2 cut(s) 127, 308
MnlI CCTC 5 cut(s) 65, 151, 177, 181, 361
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 5 cut(s) 170, 285, 297, 345, 773
MspA1I CMGCKG 2 cut(s) 554, 669
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 2 cut(s) 591, 613
MunI CAATTG 2 cut(s) 222, 321
MvaI CCWGG 2 cut(s) 591, 613
MwoI GCNNNNNNNGC 2 cut(s) 458, 497
NdeII GATC 3 cut(s) 5, 247, 595
NlaIII CATG 4 cut(s) 52, 314, 352, 681
NmuCI GTSAC 4 cut(s) 331, 508, 787, 874
NspI RCATGY 1 cut(s) 681
PciI ACATGT 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 7 cut(s) 158, 191, 202, 218, 645, 719, 821
PflMI CCANNNNNTGG 1 cut(s) 612
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 3 cut(s) 502, 762, 842
PleI GAGTC 2 cut(s) 127, 308
PpsI GAGTC 2 cut(s) 127, 308
Ppu21I YACGTR 1 cut(s) 376
PscI ACATGT 1 cut(s) 677
Psp124BI GAGCTC 1 cut(s) 562
Psp6I CCWGG 2 cut(s) 589, 611
PspFI CCCAGC 2 cut(s) 228, 669
PspGI CCWGG 2 cut(s) 589, 611
PspPI GGNCC 2 cut(s) 74, 86
PsuI RGATCY 3 cut(s) 5, 247, 595
PvuII CAGCTG 2 cut(s) 554, 669
RsaI GTAC 2 cut(s) 342, 682
RsaNI GTAC 2 cut(s) 341, 681
SacI GAGCTC 1 cut(s) 562
SaqAI TTAA 5 cut(s) 170, 285, 297, 345, 773
SatI GCNGC 3 cut(s) 501, 761, 841
Sau3AI GATC 3 cut(s) 5, 247, 595
Sau96I GGNCC 2 cut(s) 74, 86
SchI GAGTC 2 cut(s) 127, 308
ScrFI CCNGG 2 cut(s) 591, 613
SduI GDGCHC 3 cut(s) 118, 562, 677
SfaNI GCATC 1 cut(s) 92
Sse9I AATT 7 cut(s) 222, 321, 439, 745, 770, 778, 845
SsiI CCGC 1 cut(s) 760
SspMI CTAG 1 cut(s) 741
SstI GAGCTC 1 cut(s) 562
StyD4I CCNGG 2 cut(s) 589, 611
TaaI ACNGT 3 cut(s) 113, 385, 417
TaiI ACGT 2 cut(s) 35, 378
TaqI TCGA 1 cut(s) 70
TasI AATT 7 cut(s) 222, 321, 439, 745, 770, 778, 845
TatI WGTACW 1 cut(s) 680
TauI GCSGC 1 cut(s) 763
TfiI GAWTC 7 cut(s) 158, 191, 202, 218, 645, 719, 821
Tru1I TTAA 5 cut(s) 170, 285, 297, 345, 773
Tru9I TTAA 5 cut(s) 170, 285, 297, 345, 773
TscAI CASTG 3 cut(s) 390, 729, 832
TseFI GTSAC 4 cut(s) 331, 508, 787, 874
TseI GCWGC 2 cut(s) 500, 840
Tsp45I GTSAC 4 cut(s) 331, 508, 787, 874
TspDTI ATGAA 4 cut(s) 75, 231, 315, 561
TspGWI ACGGA 1 cut(s) 768
TspRI CASTG 3 cut(s) 390, 729, 832
Van91I CCANNNNNTGG 1 cut(s) 612
XagI CCTNNNNNAGG 1 cut(s) 81
XapI RAATTY 1 cut(s) 845
XceI RCATGY 1 cut(s) 681
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 1 cut(s) 741
ZraI GACGTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.