AT2G25810

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0016674
Physical Location & Seq
Forward (+)
11012499 .. 11014171
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25810.1

Sequence Viewer

Length: 750 bp
ATGAAGAAGATCGAGTTAGGGCACCATAGCGAAGCGGCTAAACCGGACTGCATTAAAGCCCTCATTGTCGAATTCATCACTACCTTTCTCTTTGTCTTCGCTGGTGTTGGATCTGCCATGGCCACTGACAGTTTAGTCGGAAACACATTGGTGGGACTTTTCGCGGTCGCGGTGGCTCATGCATTCGTTGTGGCGGTAATGATATCTGCGGGCCATATCTCCGGTGGCCACCTCAACCCCGCCGTCACCCTCGGTCTACTCTTGGGTGGCCACATCAGCGTATTCCGTGCATTCCTTTATTGGATTGATCAATTGTTGGCCTCCTCCGCAGCATGCTTCCTCCTCAGTTACCTCACCGGAGGAATGGGAACTCCGGTTCACACATTGGCAAGTGGAGTGAGTTACACGCAAGGGATCATATGGGAGATCATCTTAACATTTTCACTTCTCTTCACTGTCTACGCCACTATTGTGGATCCGAAGAAAGGATCCCTTGATGGGTTCGGCCCACTTCTCACAGGATTTGTCGTTGGAGCCAACATCCTTGCAGGAGGTGCCTTTTCTGGCGCCTCCATGAACCCCGCCCGGTCTTTTGGTCCCGCTTTGGTCTCTGGAAACTGGACTGACCATTGGGTATATTGGGTTGGACCGTTGATCGGTGGTGGACTCGCCGGTTTCATCTACGAGAATGTCCTCATTGACCGTCCACATGTCCCAGTCGCTGACGACGAGCAACCATTGTTGAATTAA

Protein Analysis

249

Amino Acids

26.07

Weight (kDa)

5.76

Isoelectric Point (pI)

23.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 13 - 228 9.2e-70 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016674)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25810
fragaria_vesca FvH4_2g35700
malus_domestica MD08G1115300.v1.1 MD15G1094400.v1.1
prunus_persica Prupe.1G449500_v2.0.a1
pyrus_communis pycom15g08720
rosa_chinensis RchiOBHm_Chr6g0306101
rosa_laevigata RLG00000010825
rosa_rugosa Rorug06G0346800
rosa_samantha Rh6AG459100 Rh6BG441800 Rh6CG473600 Rh6DG459800
rosa_wichuraiana Rw6G040020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AccB1I GGYRCC 3 cut(s) 21, 554, 566
AccI GTMKAC 2 cut(s) 256, 459
AccII CGCG 2 cut(s) 164, 170
AciI CCGC 9 cut(s) 35, 164, 170, 194, 209, 240, 327, 582, 600
AclWI GGATC 6 cut(s) 118, 422, 470, 483, 483, 496
AcoI YGGCCR 3 cut(s) 120, 226, 268
AcsI RAATTY 1 cut(s) 71
AcyI GRCGYC 1 cut(s) 567
AfiI CCNNNNNNNGG 3 cut(s) 485, 498, 656
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 1 cut(s) 745
AleI CACNNNNGTG 2 cut(s) 149, 470
AloI GAACNNNNNNTCC 2 cut(s) 360, 392
Alw26I GTCTC 1 cut(s) 613
AlwI GGATC 6 cut(s) 118, 422, 470, 483, 483, 496
AlwNI CAGNNNCTG 1 cut(s) 722
AoxI GGCC 6 cut(s) 120, 211, 226, 268, 318, 505
ApeKI GCWGC 1 cut(s) 329
ApoI RAATTY 1 cut(s) 71
AspLEI GCGC 1 cut(s) 569
AspS9I GGNCC 4 cut(s) 211, 506, 596, 647
AsuC2I CCSGG 1 cut(s) 586
AsuHPI GGTGA 2 cut(s) 238, 346
AvaII GGWCC 2 cut(s) 596, 647
BaeGI GKGCMC 1 cut(s) 24
BalI TGGCCA 3 cut(s) 122, 228, 270
BamHI GGATCC 2 cut(s) 475, 488
BanI GGYRCC 3 cut(s) 21, 554, 566
BbsI GAAGAC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 341
BccI CCATC 1 cut(s) 491
BceAI ACGGC 1 cut(s) 227
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 613
BfoI RGCGCY 1 cut(s) 570
BisI GCNGC 2 cut(s) 36, 330
BlsI GCNGC 2 cut(s) 37, 331
Bme1390I CCNGG 1 cut(s) 586
Bme18I GGWCC 2 cut(s) 596, 647
BmgT120I GGNCC 4 cut(s) 211, 506, 596, 647
BmiI GGNNCC 7 cut(s) 23, 477, 490, 535, 556, 568, 598
BmrFI CCNGG 1 cut(s) 586
BmrI ACTGGG 1 cut(s) 710
BmuI ACTGGG 1 cut(s) 710
BpiI GAAGAC 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 586
BsaHI GRCGYC 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 613
BsaJI CCNNGG 2 cut(s) 117, 250
BsaWI WCCGGW 4 cut(s) 43, 221, 356, 373
Bsc4I CCNNNNNNNGG 3 cut(s) 485, 498, 656
Bse118I RCCGGY 1 cut(s) 671
Bse1I ACTGG 2 cut(s) 623, 716
BseDI CCNNGG 2 cut(s) 117, 250
BseGI GGATG 1 cut(s) 540
BseLI CCNNNNNNNGG 3 cut(s) 485, 498, 656
BseMII CTCAG 1 cut(s) 358
BseNI ACTGG 2 cut(s) 623, 716
BseRI GAGGAG 2 cut(s) 313, 332
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 1 cut(s) 341
Bsh1236I CGCG 2 cut(s) 164, 170
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 6 cut(s) 122, 213, 228, 270, 320, 507
BshNI GGYRCC 3 cut(s) 21, 554, 566
BsiEI CGRYCG 1 cut(s) 168
BsiSI CCGG 6 cut(s) 44, 222, 357, 374, 586, 672
BslFI GGGAC 3 cut(s) 168, 582, 698
BslI CCNNNNNNNGG 3 cut(s) 485, 498, 656
BsmAI GTCTC 1 cut(s) 613
BsmFI GGGAC 3 cut(s) 168, 582, 698
BsmI GAATGC 2 cut(s) 182, 290
BsnI GGCC 6 cut(s) 122, 213, 228, 270, 320, 507
Bso31I GGTCTC 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 8 cut(s) 9, 110, 307, 414, 426, 475, 488, 654
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 9 cut(s) 35, 164, 170, 194, 209, 240, 327, 582, 600
BspANI GGCC 6 cut(s) 122, 213, 228, 270, 320, 507
BspCNI CTCAG 1 cut(s) 357
BspFNI CGCG 2 cut(s) 164, 170
BspLI GGNNCC 7 cut(s) 23, 477, 490, 535, 556, 568, 598
BspPI GGATC 6 cut(s) 118, 422, 470, 483, 483, 496
BspT107I GGYRCC 3 cut(s) 21, 554, 566
BspTNI GGTCTC 1 cut(s) 613
BsrFI RCCGGY 1 cut(s) 671
BsrI ACTGG 2 cut(s) 623, 716
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 2 cut(s) 117, 250
BssMI GATC 8 cut(s) 9, 110, 307, 414, 426, 475, 488, 654
BssNI GRCGYC 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 4 cut(s) 131, 457, 651, 704
Bst6I CTCTTC 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 567
BstAPI GCANNNNNTGC 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 211, 334
BstDEI CTNAG 1 cut(s) 344
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 540
BstFNI CGCG 2 cut(s) 164, 170
BstH2I RGCGCY 1 cut(s) 570
BstHHI GCGC 1 cut(s) 569
BstKTI GATC 8 cut(s) 12, 113, 310, 417, 429, 478, 491, 657
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 8 cut(s) 9, 110, 307, 414, 426, 475, 488, 654
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 3 cut(s) 276, 326, 554
BstNSI RCATGY 2 cut(s) 336, 713
BstSCI CCNGG 1 cut(s) 584
BstSLI GKGCMC 1 cut(s) 24
BstUI CGCG 2 cut(s) 164, 170
BstV1I GCAGC 1 cut(s) 341
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 3 cut(s) 110, 475, 488
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 3 cut(s) 110, 475, 488
BsuRI GGCC 6 cut(s) 122, 213, 228, 270, 320, 507
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 1 cut(s) 540
BtsIMutI CAGTG 2 cut(s) 123, 453
Cac8I GCNNGC 2 cut(s) 211, 334
CaiI CAGNNNCTG 1 cut(s) 722
CfoI GCGC 1 cut(s) 569
Cfr10I RCCGGY 1 cut(s) 671
Cfr13I GGNCC 4 cut(s) 211, 506, 596, 647
CviAII CATG 5 cut(s) 118, 179, 333, 574, 710
DdeI CTNAG 1 cut(s) 344
DinI GGCGCC 1 cut(s) 568
DpnI GATC 8 cut(s) 11, 112, 309, 416, 428, 477, 490, 656
DpnII GATC 8 cut(s) 9, 110, 307, 414, 426, 475, 488, 654
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 3 cut(s) 120, 226, 268
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Eco130I CCWWGG 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 613
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 596, 647
EcoRI GAATTC 1 cut(s) 71
EcoRV GATATC 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 184
EgeI GGCGCC 1 cut(s) 568
EheI GGCGCC 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 5 cut(s) 121, 182, 336, 577, 713
FalI AAGNNNNNCTT 2 cut(s) 477, 509
FaqI GGGAC 3 cut(s) 168, 582, 698
FatI CATG 5 cut(s) 117, 178, 332, 573, 709
FauI CCCGC 4 cut(s) 202, 247, 589, 607
FauNDI CATATG 1 cut(s) 419
FbaI TGATCA 1 cut(s) 307
FblI GTMKAC 2 cut(s) 256, 459
Fnu4HI GCNGC 2 cut(s) 36, 330
FokI GGATG 1 cut(s) 527
Fsp4HI GCNGC 2 cut(s) 36, 330
GlaI GCGC 1 cut(s) 568
GluI GCNGC 2 cut(s) 36, 330
HaeII RGCGCY 1 cut(s) 570
HaeIII GGCC 6 cut(s) 122, 213, 228, 270, 320, 507
HapII CCGG 6 cut(s) 44, 222, 357, 374, 586, 672
HhaI GCGC 1 cut(s) 569
Hin1I GRCGYC 1 cut(s) 567
Hin1II CATG 5 cut(s) 121, 182, 336, 577, 713
Hin6I GCGC 1 cut(s) 567
HinP1I GCGC 1 cut(s) 567
HinfI GANTC 1 cut(s) 666
HpaII CCGG 6 cut(s) 44, 222, 357, 374, 586, 672
HphI GGTGA 2 cut(s) 238, 346
Hpy166II GTNNAC 5 cut(s) 257, 379, 460, 665, 707
Hpy188I TCNGA 2 cut(s) 140, 480
Hpy188III TCNNGA 1 cut(s) 612
Hpy8I GTNNAC 5 cut(s) 257, 379, 460, 665, 707
Hpy99I CGWCG 1 cut(s) 731
HpyCH4III ACNGT 4 cut(s) 131, 457, 651, 704
HpyCH4V TGCA 4 cut(s) 51, 182, 290, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 276, 326, 554
HpyF3I CTNAG 1 cut(s) 344
Hsp92I GRCGYC 1 cut(s) 567
Hsp92II CATG 5 cut(s) 121, 182, 336, 577, 713
HspAI GCGC 1 cut(s) 567
KasI GGCGCC 1 cut(s) 566
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 8 cut(s) 9, 110, 307, 414, 426, 475, 488, 654
LmnI GCTCC 1 cut(s) 533
Lsp1109I GCAGC 1 cut(s) 341
MaeIII GTNAC 3 cut(s) 244, 347, 401
MalI GATC 8 cut(s) 11, 112, 309, 416, 428, 477, 490, 656
MboI GATC 8 cut(s) 9, 110, 307, 414, 426, 475, 488, 654
MboII GAAGA 5 cut(s) 16, 19, 88, 442, 493
MfeI CAATTG 1 cut(s) 311
MflI RGATCY 3 cut(s) 110, 475, 488
MhlI GDGCHC 1 cut(s) 24
MlsI TGGCCA 3 cut(s) 122, 228, 270
MluCI AATT 3 cut(s) 71, 311, 745
MluNI TGGCCA 3 cut(s) 122, 228, 270
Mly113I GGCGCC 1 cut(s) 567
MlyI GAGTC 1 cut(s) 660
MmeI TCCRAC 4 cut(s) 88, 118, 511, 625
Mox20I TGGCCA 3 cut(s) 122, 228, 270
Mph1103I ATGCAT 1 cut(s) 184
MscI TGGCCA 3 cut(s) 122, 228, 270
MseI TTAA 3 cut(s) 54, 434, 748
MslI CAYNNNNRTG 2 cut(s) 149, 470
Msp20I TGGCCA 3 cut(s) 122, 228, 270
MspI CCGG 6 cut(s) 44, 222, 357, 374, 586, 672
MspR9I CCNGG 1 cut(s) 586
MunI CAATTG 1 cut(s) 311
Mva1269I GAATGC 2 cut(s) 182, 290
MvnI CGCG 2 cut(s) 164, 170
MwoI GCNNNNNNNGC 3 cut(s) 276, 326, 554
NarI GGCGCC 1 cut(s) 567
NciI CCSGG 1 cut(s) 586
NcoI CCATGG 1 cut(s) 117
NdeI CATATG 1 cut(s) 419
NdeII GATC 8 cut(s) 9, 110, 307, 414, 426, 475, 488, 654
NlaIII CATG 5 cut(s) 121, 182, 336, 577, 713
NlaIV GGNNCC 7 cut(s) 23, 477, 490, 535, 556, 568, 598
NmuCI GTSAC 1 cut(s) 244
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 2 cut(s) 336, 713
OliI CACNNNNGTG 2 cut(s) 149, 470
PaeI GCATGC 1 cut(s) 336
PciI ACATGT 1 cut(s) 709
PcsI WCGNNNNNNNCGW 1 cut(s) 726
PctI GAATGC 2 cut(s) 182, 290
PkrI GCNGC 2 cut(s) 37, 331
PleI GAGTC 1 cut(s) 660
PluTI GGCGCC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 660
PscI ACATGT 1 cut(s) 709
PspN4I GGNNCC 7 cut(s) 23, 477, 490, 535, 556, 568, 598
PspPI GGNCC 4 cut(s) 211, 506, 596, 647
PstNI CAGNNNCTG 1 cut(s) 722
PsuI RGATCY 3 cut(s) 110, 475, 488
RseI CAYNNNNRTG 2 cut(s) 149, 470
SaqAI TTAA 3 cut(s) 54, 434, 748
SatI GCNGC 2 cut(s) 36, 330
Sau3AI GATC 8 cut(s) 9, 110, 307, 414, 426, 475, 488, 654
Sau96I GGNCC 4 cut(s) 211, 506, 596, 647
SchI GAGTC 1 cut(s) 660
ScrFI CCNGG 1 cut(s) 586
SduI GDGCHC 1 cut(s) 24
SetI ASST 4 cut(s) 86, 234, 354, 556
SfoI GGCGCC 1 cut(s) 568
SinI GGWCC 2 cut(s) 596, 647
SmiMI CAYNNNNRTG 2 cut(s) 149, 470
SphI GCATGC 1 cut(s) 336
Sse9I AATT 3 cut(s) 71, 311, 745
SsiI CCGC 9 cut(s) 35, 164, 170, 194, 209, 240, 327, 582, 600
SspDI GGCGCC 1 cut(s) 566
StyD4I CCNGG 1 cut(s) 584
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 4 cut(s) 131, 457, 651, 704
TaqI TCGA 2 cut(s) 12, 69
TaqII GACCGA 1 cut(s) 242
TasI AATT 3 cut(s) 71, 311, 745
TauI GCSGC 1 cut(s) 38
Tru1I TTAA 3 cut(s) 54, 434, 748
Tru9I TTAA 3 cut(s) 54, 434, 748
TscAI CASTG 2 cut(s) 130, 460
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 4 cut(s) 17, 64, 590, 667
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 2 cut(s) 130, 460
VpaK11BI GGWCC 2 cut(s) 596, 647
XapI RAATTY 1 cut(s) 71
XceI RCATGY 2 cut(s) 336, 713
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
XmiI GTMKAC 2 cut(s) 256, 459
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.