Rorug06G0346800

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002862
Physical Location & Seq
Forward (+)
49758469 .. 49761990
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0346800.1

Sequence Viewer

Length: 1455 bp
ATGGCCTGGTATCAGACAATTCCAGATTCTGATCAGAGCATTTCTGATGAGGAAGAACCGGACCAAAATTGGGCGAACAAATGCGCTACGCTCGGCGGAACCACAGAGAAAAAGGAATTGTTACAAATTTCATCTCGGCTAGAAATGCTCAAAGGTGCGAGTGCGAAGGCTTGCAATTTGGAAGATGAAGTTAGCTCTGACGATGAGGTGAACAATGTGGTGTCAAAAGTTTCTGTTAATGGCGATGCTAAAGAGTTGAGAAAAAAAGAGGATGTTCATAGCTTTTGGAGCGATAAACAAGACGAATTACACTCGTGGTCTGCTGCGAGTAAAGAAGCTGAGGCACTCATGCATTTGAATGAAAAAACTTCTTGCTCATCGTCACTGTCTGCTTACTCGAATTTAAAGAAATCGTCTAAAGGAGCCAAAGGCAAAGGCAAACCAAAATTTTTGTTCCGTTTTCCGACACACAAGGAAGGAACCTCGTCCCATTCTATATCTAAGAATGATAGTGACGTGTCATTCAGGGTTCAAGAACTGCCTGAAAGGTTGGATGCAATTGAGCCTAGAAATGTAGATCATTCAGATGTTGAACTTATTGAGGATATTCAGGGGGAAGAGGAATCAGAGATTGTGCTTAGAAATGAAGAACATTCAGATGTTGAACTTCTTGAGGACATTTCAGGGAAAGAGGAAAGTTTGTTAGAGGTTGTGCCTTTTGAAGCCAAGGCTATTCGGCATGGATGTACGGGGCAGTCAATGGCTGAGCTTCTAGATGGCCTTCAAGATAAGACTACTATTTGGAGAAAAAGTTCCAGAAAGCATAGCAGAAAACGACGTAAAAGGGAGCAGCCTGTTGTAAAGTGTGCATCTCCTCTGGGAGATGGAACTATTGACATTGAAAGCTCTCCTGAGCACTTGGGTCATGGATTGCCAAGTGATATCAAGATTGATGATCAAATTTTGAAGCTTGCTGATCCAGAAATGAAGAGGCAGACATTGGTAGATCGATTTCAGGAAGCTATGAGTGACAGACTCCTTGTTGCAGTGCCCAAAACTTTAAAGAGTGGATTATTTGGGAAACTGCAGCAGGTGGTGCAGAGTGAGAAAGAAACTGATATAGAATTCTTGAAGAAGATCCAGGAGGGAGCTAGCGAAAGTAATGACCCAAACTGCAAGGATGTGAAGATTCTTTCAAAATACCTGGATGCAAAGCTTACCGTGTGTCACTGCTCCTTCGGTAATAACTCAAAGCGCCTCCCATCGCCAGAGGTCTGCGAAGAAATGGTTGACGAAGGACTGGAAAGGACTATTATTTTCAATCCACGAGTTTGCAATGATATTGACCTTGATATTGGGAAATGGATTCGTATACACCCTCCATGGAGGGAGATCCGTGTCGGCACTGCTCAAAACATAATCCTTTCGACATATTTCTCTGAAATTTCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

484

Amino Acids

54.4

Weight (kDa)

5.21

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1081
Acc36I ACCTGC 1 cut(s) 1081
AccI GTMKAC 1 cut(s) 1372
AciI CCGC 1 cut(s) 96
AclWI GGATC 3 cut(s) 971, 1132, 1387
AcsI RAATTY 6 cut(s) 126, 400, 446, 960, 1124, 1443
AfaI GTAC 1 cut(s) 748
AfiI CCNNNNNNNGG 1 cut(s) 70
AflIII ACRYGT 1 cut(s) 516
AjiI CACGTC 1 cut(s) 517
AjnI CCWGG 3 cut(s) 5, 1140, 1203
AloI GAACNNNNNNTCC 2 cut(s) 796, 828
AluBI AGCT 9 cut(s) 195, 282, 338, 769, 906, 970, 1022, 1151, 1216
AluI AGCT 9 cut(s) 195, 282, 338, 769, 906, 970, 1022, 1151, 1216
Alw21I GWGCWC 1 cut(s) 918
AlwI GGATC 3 cut(s) 971, 1132, 1387
AlwNI CAGNNNCTG 1 cut(s) 29
AoxI GGCC 2 cut(s) 3, 778
ApeKI GCWGC 3 cut(s) 323, 850, 1087
ApoI RAATTY 6 cut(s) 126, 400, 446, 960, 1124, 1443
Asp700I GAANNNNTTC 1 cut(s) 811
AspLEI GCGC 2 cut(s) 86, 1257
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 1 cut(s) 220
AsuNHI GCTAGC 1 cut(s) 1151
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 1053
BauI CACGAG 2 cut(s) 313, 1326
Bbv12I GWGCWC 1 cut(s) 918
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 310, 862, 1099
BccI CCATC 3 cut(s) 770, 878, 1270
BciT130I CCWGG 3 cut(s) 7, 1142, 1205
BclI TGATCA 2 cut(s) 31, 955
BfaI CTAG 4 cut(s) 140, 567, 773, 1152
BfmI CTRYAG 1 cut(s) 1085
BfoI RGCGCY 1 cut(s) 1258
BfuAI ACCTGC 1 cut(s) 1081
BisI GCNGC 3 cut(s) 324, 851, 1088
BlpI GCTNAGC 1 cut(s) 765
BlsI GCNGC 3 cut(s) 325, 852, 1089
Bme1390I CCNGG 3 cut(s) 7, 1142, 1205
Bme18I GGWCC 1 cut(s) 61
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 3 cut(s) 100, 424, 481
BmrFI CCNGG 3 cut(s) 7, 1142, 1205
BmsI GCATC 4 cut(s) 235, 544, 878, 1198
BmtI GCTAGC 1 cut(s) 1155
Bpu10I CCTNAGC 2 cut(s) 339, 912
Bpu1102I GCTNAGC 1 cut(s) 765
BpuEI CTTGAG 1 cut(s) 692
Bsa29I ATCGAT 1 cut(s) 1009
BsaBI GATNNNNATC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 726, 1382
BsaWI WCCGGW 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 796, 826
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 1305
Bse3DI GCAATG 1 cut(s) 1342
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 3 cut(s) 7, 1142, 1205
BseCI ATCGAT 1 cut(s) 1009
BseDI CCNNGG 2 cut(s) 726, 1382
BseGI GGATG 5 cut(s) 277, 559, 749, 1186, 1213
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMI GCAATG 1 cut(s) 1342
BseMII CTCAG 3 cut(s) 330, 756, 903
BseNI ACTGG 1 cut(s) 1305
BseRI GAGGAG 1 cut(s) 864
BseSI GKGCMC 1 cut(s) 1053
BseXI GCAGC 3 cut(s) 310, 862, 1099
BsgI GTGCAG 1 cut(s) 1118
BshFI GGCC 2 cut(s) 5, 780
BshVI ATCGAT 1 cut(s) 1009
BsiHKAI GWGCWC 1 cut(s) 918
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 472
BslI CCNNNNNNNGG 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 472
BsnI GGCC 2 cut(s) 5, 780
Bsp1286I GDGCHC 2 cut(s) 918, 1053
Bsp143I GATC 7 cut(s) 31, 577, 955, 976, 1006, 1137, 1392
Bsp1720I GCTNAGC 1 cut(s) 765
Bsp19I CCATGG 1 cut(s) 1382
BspACI CCGC 1 cut(s) 96
BspANI GGCC 2 cut(s) 5, 780
BspCNI CTCAG 3 cut(s) 331, 757, 904
BspDI ATCGAT 1 cut(s) 1009
BspLI GGNNCC 3 cut(s) 100, 424, 481
BspMAI CTGCAG 1 cut(s) 1089
BspMI ACCTGC 1 cut(s) 1081
BspOI GCTAGC 1 cut(s) 1155
BspPI GGATC 3 cut(s) 971, 1132, 1387
BsrDI GCAATG 1 cut(s) 1342
BsrI ACTGG 1 cut(s) 1305
BssECI CCNNGG 2 cut(s) 726, 1382
BssMI GATC 7 cut(s) 31, 577, 955, 976, 1006, 1137, 1392
BssNAI GTATAC 1 cut(s) 1373
BssSI CACGAG 2 cut(s) 313, 1326
BssT1I CCWWGG 2 cut(s) 726, 1382
Bst1107I GTATAC 1 cut(s) 1373
Bst2BI CACGAG 2 cut(s) 313, 1326
Bst2UI CCWGG 3 cut(s) 7, 1142, 1205
Bst4CI ACNGT 2 cut(s) 387, 1222
Bst6I CTCTTC 2 cut(s) 612, 983
BstAPI GCANNNNNTGC 1 cut(s) 1096
BstC8I GCNNGC 3 cut(s) 172, 972, 1153
BstDEI CTNAG 5 cut(s) 339, 501, 638, 765, 912
BstDSI CCRYGG 1 cut(s) 1382
BstF5I GGATG 5 cut(s) 277, 559, 749, 1186, 1213
BstH2I RGCGCY 1 cut(s) 1258
BstHHI GCGC 2 cut(s) 86, 1257
BstKTI GATC 7 cut(s) 34, 580, 958, 979, 1009, 1140, 1395
BstMBI GATC 7 cut(s) 31, 577, 955, 976, 1006, 1137, 1392
BstMWI GCNNNNNNNGC 3 cut(s) 145, 288, 1096
BstNI CCWGG 3 cut(s) 7, 1142, 1205
BstSCI CCNGG 3 cut(s) 5, 1140, 1203
BstSFI CTRYAG 1 cut(s) 1085
BstSLI GKGCMC 1 cut(s) 1053
BstV1I GCAGC 3 cut(s) 310, 862, 1099
BstX2I RGATCY 2 cut(s) 1137, 1392
BstYI RGATCY 2 cut(s) 1137, 1392
BstZ17I GTATAC 1 cut(s) 1373
Bsu15I ATCGAT 1 cut(s) 1009
BsuRI GGCC 2 cut(s) 5, 780
BsuTUI ATCGAT 1 cut(s) 1009
BtgI CCRYGG 1 cut(s) 1382
BtgZI GCGATG 2 cut(s) 258, 1248
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 5 cut(s) 277, 559, 749, 1186, 1213
BtsI GCAGTG 3 cut(s) 1053, 1228, 1404
BtsIMutI CAGTG 4 cut(s) 383, 1053, 1228, 1404
BveI ACCTGC 1 cut(s) 1081
Cac8I GCNNGC 3 cut(s) 172, 972, 1153
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 2 cut(s) 86, 1257
Cfr13I GGNCC 1 cut(s) 61
ClaI ATCGAT 1 cut(s) 1009
Csp6I GTAC 1 cut(s) 747
CviAII CATG 4 cut(s) 349, 740, 926, 1383
CviQI GTAC 1 cut(s) 747
DdeI CTNAG 5 cut(s) 339, 501, 638, 765, 912
DpnI GATC 7 cut(s) 33, 579, 957, 978, 1008, 1139, 1394
DpnII GATC 7 cut(s) 31, 577, 955, 976, 1006, 1137, 1392
DraI TTTAAA 2 cut(s) 405, 1062
Eam1104I CTCTTC 2 cut(s) 612, 983
EarI CTCTTC 2 cut(s) 612, 983
EciI GGCGGA 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 726, 1382
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 1124
EcoRII CCWGG 3 cut(s) 5, 1140, 1203
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 2 cut(s) 726, 1382
EcoT22I ATGCAT 1 cut(s) 354
ErhI CCWWGG 2 cut(s) 726, 1382
FaeI CATG 4 cut(s) 352, 743, 929, 1386
FaqI GGGAC 1 cut(s) 472
FatI CATG 4 cut(s) 348, 739, 925, 1382
FbaI TGATCA 2 cut(s) 31, 955
FblI GTMKAC 1 cut(s) 1372
Fnu4HI GCNGC 3 cut(s) 324, 851, 1088
FokI GGATG 5 cut(s) 284, 566, 756, 1193, 1220
Fsp4HI GCNGC 3 cut(s) 324, 851, 1088
FspBI CTAG 4 cut(s) 140, 567, 773, 1152
GlaI GCGC 2 cut(s) 85, 1256
GluI GCNGC 3 cut(s) 324, 851, 1088
HaeII RGCGCY 1 cut(s) 1258
HaeIII GGCC 2 cut(s) 5, 780
HapII CCGG 1 cut(s) 59
HhaI GCGC 2 cut(s) 86, 1257
Hin1II CATG 4 cut(s) 352, 743, 929, 1386
Hin6I GCGC 2 cut(s) 84, 1255
HinP1I GCGC 2 cut(s) 84, 1255
HincII GTYRAC 1 cut(s) 1291
HindII GTYRAC 1 cut(s) 1291
HindIII AAGCTT 2 cut(s) 968, 1214
HinfI GANTC 5 cut(s) 26, 623, 1035, 1189, 1366
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 3 cut(s) 211, 1291, 1373
Hpy8I GTNNAC 3 cut(s) 211, 1291, 1373
Hpy99I CGWCG 1 cut(s) 840
HpyAV CCTTC 5 cut(s) 160, 470, 791, 1246, 1289
HpyCH4III ACNGT 2 cut(s) 387, 1222
HpyCH4IV ACGT 2 cut(s) 516, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 288, 1096
HpyF3I CTNAG 5 cut(s) 339, 501, 638, 765, 912
HpySE526I ACGT 2 cut(s) 516, 838
Hsp92II CATG 4 cut(s) 352, 743, 929, 1386
HspAI GCGC 2 cut(s) 84, 1255
Ksp22I TGATCA 2 cut(s) 31, 955
Kzo9I GATC 7 cut(s) 31, 577, 955, 976, 1006, 1137, 1392
LmnI GCTCC 5 cut(s) 288, 422, 847, 1148, 1238
Lsp1109I GCAGC 3 cut(s) 310, 862, 1099
LweI GCATC 4 cut(s) 235, 544, 878, 1198
MaeI CTAG 4 cut(s) 140, 567, 773, 1152
MaeII ACGT 2 cut(s) 516, 838
MaeIII GTNAC 5 cut(s) 120, 381, 512, 1028, 1226
MalI GATC 7 cut(s) 33, 579, 957, 978, 1008, 1139, 1394
MboI GATC 7 cut(s) 31, 577, 955, 976, 1006, 1137, 1392
MboII GAAGA 9 cut(s) 65, 194, 629, 659, 1000, 1144, 1147, 1198, 1292
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 2 cut(s) 1137, 1392
MhlI GDGCHC 2 cut(s) 918, 1053
MlyI GAGTC 1 cut(s) 1029
MmeI TCCRAC 2 cut(s) 488, 531
Mph1103I ATGCAT 1 cut(s) 354
MroXI GAANNNNTTC 1 cut(s) 811
MseI TTAA 3 cut(s) 237, 404, 1061
MslI CAYNNNNRTG 3 cut(s) 357, 585, 657
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 3 cut(s) 7, 1142, 1205
MunI CAATTG 1 cut(s) 558
MvaI CCWGG 3 cut(s) 7, 1142, 1205
MwoI GCNNNNNNNGC 3 cut(s) 145, 288, 1096
NcoI CCATGG 1 cut(s) 1382
NdeII GATC 7 cut(s) 31, 577, 955, 976, 1006, 1137, 1392
NheI GCTAGC 1 cut(s) 1151
NlaIII CATG 4 cut(s) 352, 743, 929, 1386
NlaIV GGNNCC 3 cut(s) 100, 424, 481
NmeAIII GCCGAG 2 cut(s) 72, 115
NmuCI GTSAC 4 cut(s) 381, 512, 1028, 1226
NsiI ATGCAT 1 cut(s) 354
PaqCI CACCTGC 1 cut(s) 1081
PdmI GAANNNNTTC 1 cut(s) 811
PfeI GAWTC 4 cut(s) 26, 623, 1189, 1366
PfoI TCCNGGA 1 cut(s) 1140
PkrI GCNGC 3 cut(s) 325, 852, 1089
PleI GAGTC 1 cut(s) 1029
PpsI GAGTC 1 cut(s) 1029
Psp6I CCWGG 3 cut(s) 5, 1140, 1203
PspGI CCWGG 3 cut(s) 5, 1140, 1203
PspN4I GGNNCC 3 cut(s) 100, 424, 481
PspPI GGNCC 1 cut(s) 61
PstI CTGCAG 1 cut(s) 1089
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 2 cut(s) 1137, 1392
RsaI GTAC 1 cut(s) 748
RsaNI GTAC 1 cut(s) 747
RseI CAYNNNNRTG 3 cut(s) 357, 585, 657
SaqAI TTAA 3 cut(s) 237, 404, 1061
SatI GCNGC 3 cut(s) 324, 851, 1088
Sau3AI GATC 7 cut(s) 31, 577, 955, 976, 1006, 1137, 1392
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 1 cut(s) 1029
ScrFI CCNGG 3 cut(s) 7, 1142, 1205
SduI GDGCHC 2 cut(s) 918, 1053
SfaNI GCATC 4 cut(s) 235, 544, 878, 1198
SfcI CTRYAG 1 cut(s) 1085
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 3 cut(s) 357, 585, 657
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
SsiI CCGC 1 cut(s) 96
SspMI CTAG 4 cut(s) 140, 567, 773, 1152
StyD4I CCNGG 3 cut(s) 5, 1140, 1203
StyI CCWWGG 2 cut(s) 726, 1382
TaaI ACNGT 2 cut(s) 387, 1222
TaiI ACGT 2 cut(s) 519, 841
TaqI TCGA 3 cut(s) 398, 1009, 1427
TfiI GAWTC 4 cut(s) 26, 623, 1189, 1366
Tru1I TTAA 3 cut(s) 237, 404, 1061
Tru9I TTAA 3 cut(s) 237, 404, 1061
TscAI CASTG 4 cut(s) 390, 1053, 1235, 1411
TseFI GTSAC 4 cut(s) 381, 512, 1028, 1226
TseI GCWGC 3 cut(s) 323, 850, 1087
Tsp45I GTSAC 4 cut(s) 381, 512, 1028, 1226
TspDTI ATGAA 6 cut(s) 120, 201, 266, 375, 660, 1001
TspGWI ACGGA 2 cut(s) 446, 1385
TspRI CASTG 4 cut(s) 390, 1053, 1235, 1411
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 6 cut(s) 126, 400, 446, 960, 1124, 1443
XbaI TCTAGA 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 1372
XmnI GAANNNNTTC 1 cut(s) 811
XspI CTAG 4 cut(s) 140, 567, 773, 1152
Zsp2I ATGCAT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.