Rw6G040040

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002862
Physical Location & Seq
Reverse (-)
63864565 .. 63874849
10285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040040.1

Sequence Viewer

Length: 1779 bp
ATGGCAATCTCTTCGTATAATGCTTCACAAGTAAACAGGTCATTGCAAAGCCGTCTCATCTCCCACAGCTTTAATCATCTCAAAACCTCACTTTCCTCAACTGTTAAGCCTTTCTTCAAAGAGTTGCAGCAATTGCTTCCGATGAAGGTGGATGTCTCGAAAACCATGAAGAACACTTCCGGGAAAATGTTAGATGCAGTTGTGGACTCCCTGTTCCAATTTGTGGATAAAAACTTTTTACCTGTTGAAGAGATTGGAGAACTTGTTGAGGTCACCTGCACCTTAGGAGAAATTCCTGCTGATTTTGCTGAGGGTGTTTTCATCAGAAATGGCAGTAATCCTTTGTTTGGAGGTCTAAAATCAGCAGTTTCGATCTTCGGAGAATCACACAATATTTGGGTAGAGGGAGAAGGAATGCTCCATGCTCTATACTTCAAGAAAGACGATGATTGTGGAAGTTGGATCATCTCATATAAGAATAGATATGTTGAGAGTGAGACATTTAAGATAGAAACTCAACGAAATAAACCAGGTTTCTTACCGGCCCTTGCAGGAGACGCTCCAGCAATTATAGCAGCATTTCTTTTAAATTCGTTGAGATTTGGGACAGTCATTAAACACATGAGCAACACCAATGTTTTCGAGCATTCAGGGAGAATTTACTCAATTGCAGAGAACTATCTGCCTCAAGAGGTGGACATTTCAACCTTGGAAACCATTTGTGATTGGGATGTGAATGGAACTTGGGATCGACCTTTCACAAGTCATCCAAAGAAAGCTCCAAACGGAGAGTTAGTCGTGATGGGGACAGATGCATGGAAACCTTACTATGTTTTAGGGGTCATCTCTGCTGATGGAAAGAAAGTGCACAAGGTTGATCTCAAATTTAAAAGATGTGTGTTCAGTCATGATATAGGAGTCACGCAAAACTACAATGTAATCCTTGATCATCCCCTCACATTGGATGTAAAGAGACTCACCATGGGAGGCCAAATAATGCAGTATGAAAAGGAAGAGTACGCTAGAATTGGGGTCATGCCTCGTTATGGAGATGCAGAGTCTGTCAAATGGTTTGAGGTACAGACCAATTGCACACTTCACTTTCTCAATTGCTTTGAGGATGCTAATCAGGTTATAGTCAGGGGATGTAGGGCTCTCACATCTGTTGTATCAGATCCTGACGGCAGCCTAAACCCTTATGAATGGTTCTCCAAAGGGTTCAACTTTGCTGGTGATCATGATTCAGCAGAGGTTGGATATCTCTTTACGCATTTATACGAATGGAGATTGAACATGTTATCTGGGAAAGTTGAGGAGAGAAACTTAACTGGATCCGAATACTCCATGGATTTCCCTTTCATCAATGAACAATTTACTGGTTTAAAACACAAGTATGGATATACTCAGGTCATTGATTCCGGGGCAAGCTCTATATGTGGCATGGCTAAATTTGGATCTCTAGCCAAATTATATTTGGAAGAATCAGATTCTACAAAATCTGAGGGAAGGGGTGAGCACTTGATAAAGGTGGAATACCATAAGTTTGAGGACAACAACTTTTGCAGTGGAAGTGCTTTTGTCCCCAGAAATGGAGGCATCGAGGAAGATGATGGCTGGATTATCACTTTTGTCCACAATGAAGAAACTGATGTGACCCAAGTTCATATCATTGATGCACAGGATTTTGAAGGTGAAGCGATTGCGAAACTTACATTGCCACAACGGGTGCCCTATGGTTTTCATTCAACCTTTCTGTCAATGCCAGCTTGTCAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

592

Amino Acids

66.54

Weight (kDa)

5.34

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 82 - 585 9.4e-110 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 1726
AccB7I CCANNNNNTGG 1 cut(s) 223
AclWI GGATC 6 cut(s) 470, 756, 1169, 1326, 1339, 1462
AcsI RAATTY 5 cut(s) 291, 589, 657, 884, 1448
AfaI GTAC 2 cut(s) 1019, 1080
AfiI CCNNNNNNNGG 5 cut(s) 223, 347, 961, 1046, 1589
AflIII ACRYGT 1 cut(s) 1293
AgsI TTSAA 8 cut(s) 118, 248, 436, 705, 1222, 1291, 1688, 1746
AjnI CCWGG 1 cut(s) 529
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 4 cut(s) 69, 779, 1428, 1766
AluI AGCT 4 cut(s) 69, 779, 1428, 1766
Alw21I GWGCWC 2 cut(s) 870, 1518
Alw26I GTCTC 5 cut(s) 59, 160, 491, 549, 967
Alw44I GTGCAC 1 cut(s) 866
AlwI GGATC 6 cut(s) 470, 756, 1169, 1326, 1339, 1462
AlwNI CAGNNNCTG 2 cut(s) 1061, 1178
AoxI GGCC 2 cut(s) 543, 988
ApaLI GTGCAC 1 cut(s) 866
ApeKI GCWGC 3 cut(s) 127, 575, 1185
ApoI RAATTY 5 cut(s) 291, 589, 657, 884, 1448
AspS9I GGNCC 1 cut(s) 544
AsuC2I CCSGG 2 cut(s) 181, 1420
AsuHPI GGTGA 5 cut(s) 265, 970, 1244, 1523, 1703
AxyI CCTNAGG 1 cut(s) 283
BaeGI GKGCMC 2 cut(s) 870, 1731
BamHI GGATCC 1 cut(s) 1331
BanI GGYRCC 1 cut(s) 1726
BanII GRGCYC 1 cut(s) 1156
Bbv12I GWGCWC 2 cut(s) 870, 1518
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 3 cut(s) 139, 587, 1197
BccI CCATC 3 cut(s) 796, 848, 1604
BceAI ACGGC 2 cut(s) 36, 1198
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 531
BclI TGATCA 2 cut(s) 946, 1234
BcnI CCSGG 2 cut(s) 181, 1420
BcoDI GTCTC 5 cut(s) 59, 160, 491, 549, 967
BfaI CTAG 2 cut(s) 1023, 1460
BfuAI ACCTGC 1 cut(s) 284
BisI GCNGC 3 cut(s) 128, 576, 1186
BlsI GCNGC 3 cut(s) 129, 577, 1187
Bme1390I CCNGG 3 cut(s) 181, 531, 1420
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 2 cut(s) 1333, 1728
BmrFI CCNGG 3 cut(s) 181, 531, 1420
BmsI GCATC 6 cut(s) 184, 802, 1042, 1111, 1605, 1663
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 672
BpuMI CCSGG 2 cut(s) 181, 1420
BsaBI GATNNNNATC 1 cut(s) 1125
BsaJI CCNNGG 4 cut(s) 708, 981, 1344, 1419
BsaXI ACNNNNNCTCC 4 cut(s) 249, 279, 780, 810
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 347, 961, 1046, 1589
Bse118I RCCGGY 1 cut(s) 541
Bse1I ACTGG 2 cut(s) 1333, 1381
Bse21I CCTNAGG 1 cut(s) 283
Bse3DI GCAATG 2 cut(s) 41, 1712
Bse8I GATNNNNATC 1 cut(s) 1125
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 4 cut(s) 708, 981, 1344, 1419
BseGI GGATG 7 cut(s) 157, 736, 766, 949, 970, 1126, 1151
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 5 cut(s) 223, 347, 961, 1046, 1589
BseMI GCAATG 2 cut(s) 41, 1712
BseMII CTCAG 3 cut(s) 300, 1418, 1491
BseNI ACTGG 2 cut(s) 1333, 1381
BseRI GAGGAG 1 cut(s) 1328
BseSI GKGCMC 2 cut(s) 870, 1731
BseXI GCAGC 3 cut(s) 139, 587, 1197
BsgI GTGCAG 1 cut(s) 262
BshFI GGCC 2 cut(s) 545, 990
BshNI GGYRCC 1 cut(s) 1726
BsiHKAI GWGCWC 2 cut(s) 870, 1518
BsiSI CCGG 3 cut(s) 180, 542, 1419
BslFI GGGAC 3 cut(s) 619, 820, 1565
BslI CCNNNNNNNGG 5 cut(s) 223, 347, 961, 1046, 1589
BsmAI GTCTC 5 cut(s) 59, 160, 491, 549, 967
BsmBI CGTCTC 2 cut(s) 59, 549
BsmFI GGGAC 3 cut(s) 619, 820, 1565
BsmI GAATGC 2 cut(s) 420, 646
BsnI GGCC 2 cut(s) 545, 990
Bsp1286I GDGCHC 4 cut(s) 870, 1156, 1518, 1731
Bsp143I GATC 9 cut(s) 372, 462, 748, 877, 946, 1174, 1234, 1331, 1454
Bsp19I CCATGG 2 cut(s) 981, 1344
BspANI GGCC 2 cut(s) 545, 990
BspCNI CTCAG 3 cut(s) 301, 1417, 1492
BspHI TCATGA 2 cut(s) 907, 1237
BspLI GGNNCC 2 cut(s) 1333, 1728
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 6 cut(s) 470, 756, 1169, 1326, 1339, 1462
BspT107I GGYRCC 1 cut(s) 1726
BsrDI GCAATG 2 cut(s) 41, 1712
BsrFI RCCGGY 1 cut(s) 541
BsrI ACTGG 2 cut(s) 1333, 1381
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 4 cut(s) 708, 981, 1344, 1419
BssMI GATC 9 cut(s) 372, 462, 748, 877, 946, 1174, 1234, 1331, 1454
BssT1I CCWWGG 3 cut(s) 708, 981, 1344
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 2 cut(s) 103, 610
Bst6I CTCTTC 3 cut(s) 16, 243, 1008
BstAPI GCANNNNNTGC 1 cut(s) 133
BstC8I GCNNGC 2 cut(s) 1426, 1764
BstDEI CTNAG 5 cut(s) 283, 309, 1404, 1500, 1776
BstDSI CCRYGG 2 cut(s) 981, 1344
BstEII GGTNACC 1 cut(s) 271
BstF5I GGATG 7 cut(s) 157, 736, 766, 949, 970, 1126, 1151
BstKTI GATC 9 cut(s) 375, 465, 751, 880, 949, 1177, 1237, 1334, 1457
BstMAI GTCTC 5 cut(s) 59, 160, 491, 549, 967
BstMBI GATC 9 cut(s) 372, 462, 748, 877, 946, 1174, 1234, 1331, 1454
BstMWI GCNNNNNNNGC 4 cut(s) 133, 305, 557, 572
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 1 cut(s) 1297
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 3 cut(s) 179, 529, 1418
BstSLI GKGCMC 2 cut(s) 870, 1731
BstV1I GCAGC 3 cut(s) 139, 587, 1197
BstX2I RGATCY 3 cut(s) 1174, 1331, 1454
BstYI RGATCY 3 cut(s) 1174, 1331, 1454
Bsu36I CCTNAGG 1 cut(s) 283
BsuRI GGCC 2 cut(s) 545, 990
BtgI CCRYGG 2 cut(s) 981, 1344
BtsCI GGATG 7 cut(s) 157, 736, 766, 949, 970, 1126, 1151
BtsI GCAGTG 1 cut(s) 1570
BtsIMutI CAGTG 1 cut(s) 1570
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 2 cut(s) 1426, 1764
CaiI CAGNNNCTG 2 cut(s) 1061, 1178
CciI TCATGA 2 cut(s) 907, 1237
Cfr10I RCCGGY 1 cut(s) 541
Cfr13I GGNCC 1 cut(s) 544
CseI GACGC 1 cut(s) 566
CsiI ACCWGGT 1 cut(s) 529
Csp6I GTAC 2 cut(s) 1018, 1079
CviQI GTAC 2 cut(s) 1018, 1079
DdeI CTNAG 5 cut(s) 283, 309, 1404, 1500, 1776
DpnI GATC 9 cut(s) 374, 464, 750, 879, 948, 1176, 1236, 1333, 1456
DpnII GATC 9 cut(s) 372, 462, 748, 877, 946, 1174, 1234, 1331, 1454
DraI TTTAAA 3 cut(s) 588, 889, 1383
Eam1104I CTCTTC 3 cut(s) 16, 243, 1008
EarI CTCTTC 3 cut(s) 16, 243, 1008
Eco130I CCWWGG 3 cut(s) 708, 981, 1344
Eco24I GRGCYC 1 cut(s) 1156
Eco32I GATATC 1 cut(s) 1259
Eco81I CCTNAGG 1 cut(s) 283
Eco91I GGTNACC 1 cut(s) 271
EcoO65I GGTNACC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 1259
EcoT14I CCWWGG 3 cut(s) 708, 981, 1344
EcoT22I ATGCAT 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 1156
ErhI CCWWGG 3 cut(s) 708, 981, 1344
Esp3I CGTCTC 2 cut(s) 59, 549
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 3 cut(s) 619, 820, 1565
FbaI TGATCA 2 cut(s) 946, 1234
Fnu4HI GCNGC 3 cut(s) 128, 576, 1186
FokI GGATG 7 cut(s) 164, 743, 753, 936, 977, 1133, 1158
FriOI GRGCYC 1 cut(s) 1156
Fsp4HI GCNGC 3 cut(s) 128, 576, 1186
FspBI CTAG 2 cut(s) 1023, 1460
GluI GCNGC 3 cut(s) 128, 576, 1186
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 2 cut(s) 545, 990
HapII CCGG 3 cut(s) 180, 542, 1419
HgaI GACGC 1 cut(s) 566
HinfI GANTC 9 cut(s) 206, 383, 918, 975, 1058, 1241, 1415, 1481, 1487
HpaII CCGG 3 cut(s) 180, 542, 1419
HphI GGTGA 5 cut(s) 265, 970, 1244, 1523, 1703
Hpy166II GTNNAC 5 cut(s) 34, 205, 697, 868, 1633
Hpy188I TCNGA 7 cut(s) 141, 326, 380, 1174, 1336, 1486, 1501
Hpy188III TCNNGA 7 cut(s) 157, 436, 689, 799, 908, 1178, 1238
Hpy8I GTNNAC 5 cut(s) 34, 205, 697, 868, 1633
HpyAV CCTTC 4 cut(s) 139, 404, 1500, 1682
HpyCH4III ACNGT 2 cut(s) 103, 610
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 305, 557, 572
HpyF3I CTNAG 5 cut(s) 283, 309, 1404, 1500, 1776
Ksp22I TGATCA 2 cut(s) 946, 1234
Kzo9I GATC 9 cut(s) 372, 462, 748, 877, 946, 1174, 1234, 1331, 1454
LmnI GCTCC 3 cut(s) 423, 565, 784
Lsp1109I GCAGC 3 cut(s) 139, 587, 1197
LweI GCATC 6 cut(s) 184, 802, 1042, 1111, 1605, 1663
MabI ACCWGGT 1 cut(s) 529
MaeI CTAG 2 cut(s) 1023, 1460
MaeIII GTNAC 3 cut(s) 271, 919, 1651
MalI GATC 9 cut(s) 374, 464, 750, 879, 948, 1176, 1236, 1333, 1456
MboI GATC 9 cut(s) 372, 462, 748, 877, 946, 1174, 1234, 1331, 1454
MboII GAAGA 9 cut(s) 3, 106, 181, 260, 367, 1025, 1490, 1616, 1652
MfeI CAATTG 4 cut(s) 131, 666, 1087, 1108
MflI RGATCY 3 cut(s) 1174, 1331, 1454
MhlI GDGCHC 4 cut(s) 870, 1156, 1518, 1731
MlyI GAGTC 4 cut(s) 200, 927, 969, 1067
MmeI TCCRAC 2 cut(s) 440, 1234
Mph1103I ATGCAT 1 cut(s) 817
MseI TTAA 8 cut(s) 72, 105, 504, 587, 615, 888, 1325, 1382
MslI CAYNNNNRTG 1 cut(s) 1392
MspI CCGG 3 cut(s) 180, 542, 1419
MspR9I CCNGG 3 cut(s) 181, 531, 1420
MunI CAATTG 4 cut(s) 131, 666, 1087, 1108
Mva1269I GAATGC 2 cut(s) 420, 646
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 4 cut(s) 133, 305, 557, 572
NciI CCSGG 2 cut(s) 181, 1420
NcoI CCATGG 2 cut(s) 981, 1344
NdeII GATC 9 cut(s) 372, 462, 748, 877, 946, 1174, 1234, 1331, 1454
NlaIV GGNNCC 2 cut(s) 1333, 1728
NmuCI GTSAC 3 cut(s) 271, 919, 1651
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 1 cut(s) 1297
PagI TCATGA 2 cut(s) 907, 1237
PaqCI CACCTGC 1 cut(s) 284
PciI ACATGT 1 cut(s) 1293
PctI GAATGC 2 cut(s) 420, 646
PfeI GAWTC 5 cut(s) 383, 1241, 1415, 1481, 1487
PflMI CCANNNNNTGG 1 cut(s) 223
PfoI TCCNGGA 1 cut(s) 179
PkrI GCNGC 3 cut(s) 129, 577, 1187
PleI GAGTC 4 cut(s) 200, 926, 969, 1066
PpsI GAGTC 4 cut(s) 200, 926, 969, 1066
PscI ACATGT 1 cut(s) 1293
Psp6I CCWGG 1 cut(s) 529
PspEI GGTNACC 1 cut(s) 271
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 2 cut(s) 1333, 1728
PspPI GGNCC 1 cut(s) 544
PstNI CAGNNNCTG 2 cut(s) 1061, 1178
PsuI RGATCY 3 cut(s) 1174, 1331, 1454
RsaI GTAC 2 cut(s) 1019, 1080
RsaNI GTAC 2 cut(s) 1018, 1079
RseI CAYNNNNRTG 1 cut(s) 1392
SaqAI TTAA 8 cut(s) 72, 105, 504, 587, 615, 888, 1325, 1382
SatI GCNGC 3 cut(s) 128, 576, 1186
Sau3AI GATC 9 cut(s) 372, 462, 748, 877, 946, 1174, 1234, 1331, 1454
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 4 cut(s) 200, 927, 969, 1067
ScrFI CCNGG 3 cut(s) 181, 531, 1420
SduI GDGCHC 4 cut(s) 870, 1156, 1518, 1731
SexAI ACCWGGT 1 cut(s) 529
SfaNI GCATC 6 cut(s) 184, 802, 1042, 1111, 1605, 1663
SmiMI CAYNNNNRTG 1 cut(s) 1392
SmlI CTYRAG 1 cut(s) 687
SmoI CTYRAG 1 cut(s) 687
SspI AATATT 1 cut(s) 394
SspMI CTAG 2 cut(s) 1023, 1460
StyD4I CCNGG 3 cut(s) 179, 529, 1418
StyI CCWWGG 3 cut(s) 708, 981, 1344
TaaI ACNGT 2 cut(s) 103, 610
TaqI TCGA 5 cut(s) 158, 371, 642, 751, 1599
TfiI GAWTC 5 cut(s) 383, 1241, 1415, 1481, 1487
Tru1I TTAA 8 cut(s) 72, 105, 504, 587, 615, 888, 1325, 1382
Tru9I TTAA 8 cut(s) 72, 105, 504, 587, 615, 888, 1325, 1382
TscAI CASTG 1 cut(s) 1570
TseFI GTSAC 3 cut(s) 271, 919, 1651
TseI GCWGC 3 cut(s) 127, 575, 1185
Tsp45I GTSAC 3 cut(s) 271, 919, 1651
TspGWI ACGGA 1 cut(s) 801
TspRI CASTG 1 cut(s) 1570
Van91I CCANNNNNTGG 1 cut(s) 223
VneI GTGCAC 1 cut(s) 866
XapI RAATTY 5 cut(s) 291, 589, 657, 884, 1448
XceI RCATGY 1 cut(s) 1297
XcmI CCANNNNNNNNNTGG 1 cut(s) 1471
XspI CTAG 2 cut(s) 1023, 1460
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.