Rroxscaffold_7G00162040

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002862
Physical Location & Seq
Forward (+)
4700250 .. 4701895
1646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162040.1

Sequence Viewer

Length: 537 bp
ATGGGAGGCCAAATAATGCAGTATGAAAAGGAAGAGTACGCTAGAATTGGGGTCATGCCTCGTTATGGAGATGCAGAGTCTGTCAAATGGTTTGAGGTACAGACCAATTGCACACTTCACTTTCTCAATTGCTTTGAGGATGCTAATCAGGTTATCGTCAGGGGATGTAGGGCTCTCACATCTGTTGTATCAGATCCTGACGGCAGCCTAAACCCTTATGAATGGTTCTCCAAAGGGTTCCTCTTTGCTGGTGATCATGATTCTGCAGAGGTTGGATATCTCTTTACGCATTTATACGAATGGAGATTGAACATGTTATCTGGGAAAGTTGTGGAGAGAAACTTAACTGGATCTGAATACTCCATGGATTTCCCTTTCATCAATGAACAATTTACGGGTTTAAAACACAAGTATGGATACACTCAGGTCATTGATTCCAGGGCAAGCTCTATATGTGGCATGGCTAAATTTGGGATCTGTAGCCAAATTATATTTGGAAGAATCAGATTCTACAAAATCTGTGGAGGGAAGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

178

Amino Acids

20.26

Weight (kDa)

6.08

Isoelectric Point (pI)

16.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 2 - 157 4.3e-24 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 188, 358, 482
AcsI RAATTY 1 cut(s) 467
AfaI GTAC 2 cut(s) 38, 99
AfiI CCNNNNNNNGG 1 cut(s) 65
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 1 cut(s) 310
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 1 cut(s) 447
AluI AGCT 1 cut(s) 447
AlwI GGATC 3 cut(s) 188, 358, 482
AlwNI CAGNNNCTG 2 cut(s) 80, 197
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 1 cut(s) 204
ApoI RAATTY 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 263
BanII GRGCYC 1 cut(s) 175
BbvI GCAGC 1 cut(s) 216
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 1 cut(s) 439
BciVI GTATCC 1 cut(s) 410
BclI TGATCA 1 cut(s) 253
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 2 cut(s) 264, 478
BfuI GTATCC 1 cut(s) 410
BisI GCNGC 1 cut(s) 205
BlsI GCNGC 1 cut(s) 206
Bme1390I CCNGG 1 cut(s) 439
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 439
BmsI GCATC 2 cut(s) 61, 130
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 2 cut(s) 363, 438
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 352
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 363, 438
BseGI GGATG 2 cut(s) 145, 170
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 352
BseXI GCAGC 1 cut(s) 216
BshFI GGCC 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 65
BsnI GGCC 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 4 cut(s) 193, 253, 350, 474
Bsp19I CCATGG 1 cut(s) 363
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 1 cut(s) 436
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 239
BspMAI CTGCAG 1 cut(s) 268
BspPI GGATC 3 cut(s) 188, 358, 482
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 363, 438
BssMI GATC 4 cut(s) 193, 253, 350, 474
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 439
Bst6I CTCTTC 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 1 cut(s) 423
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 2 cut(s) 145, 170
BstKTI GATC 4 cut(s) 196, 256, 353, 477
BstMBI GATC 4 cut(s) 193, 253, 350, 474
BstNI CCWGG 1 cut(s) 439
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 437
BstSFI CTRYAG 2 cut(s) 264, 478
BstV1I GCAGC 1 cut(s) 216
BstX2I RGATCY 3 cut(s) 193, 350, 474
BstYI RGATCY 3 cut(s) 193, 350, 474
BsuI GTATCC 1 cut(s) 410
BsuRI GGCC 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 2 cut(s) 145, 170
Cac8I GCNNGC 1 cut(s) 445
CaiI CAGNNNCTG 2 cut(s) 80, 197
CciI TCATGA 1 cut(s) 256
Csp6I GTAC 2 cut(s) 37, 98
CspCI CAANNNNNGTGG 2 cut(s) 502, 537
CviAII CATG 5 cut(s) 55, 257, 313, 364, 460
CviJI RGCY 6 cut(s) 9, 173, 207, 447, 464, 483
CviKI_1 RGCY 6 cut(s) 9, 173, 207, 447, 464, 483
CviQI GTAC 2 cut(s) 37, 98
DdeI CTNAG 1 cut(s) 423
DpnI GATC 4 cut(s) 195, 255, 352, 476
DpnII GATC 4 cut(s) 193, 253, 350, 474
DraI TTTAAA 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 175
Eco32I GATATC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 437
EcoRV GATATC 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 5 cut(s) 58, 260, 316, 367, 463
FatI CATG 5 cut(s) 54, 256, 312, 363, 459
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 205
FokI GGATG 2 cut(s) 152, 177
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 1 cut(s) 205
FspBI CTAG 1 cut(s) 42
GluI GCNGC 1 cut(s) 205
HaeIII GGCC 1 cut(s) 9
Hin1II CATG 5 cut(s) 58, 260, 316, 367, 463
HinfI GANTC 5 cut(s) 77, 260, 434, 501, 507
HphI GGTGA 1 cut(s) 263
Hpy188I TCNGA 3 cut(s) 193, 355, 506
Hpy188III TCNNGA 2 cut(s) 197, 257
HpyAV CCTTC 1 cut(s) 523
HpyCH4V TGCA 4 cut(s) 19, 74, 111, 266
HpyF3I CTNAG 1 cut(s) 423
Hsp92II CATG 5 cut(s) 58, 260, 316, 367, 463
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 4 cut(s) 193, 253, 350, 474
LpnPI CCDG 9 cut(s) 134, 145, 210, 234, 306, 333, 410, 424, 451
Lsp1109I GCAGC 1 cut(s) 216
LweI GCATC 2 cut(s) 61, 130
MaeI CTAG 1 cut(s) 42
MalI GATC 4 cut(s) 195, 255, 352, 476
MboI GATC 4 cut(s) 193, 253, 350, 474
MboII GAAGA 2 cut(s) 44, 510
MfeI CAATTG 2 cut(s) 106, 127
MflI RGATCY 3 cut(s) 193, 350, 474
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 6 cut(s) 45, 106, 127, 389, 467, 486
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 6 cut(s) 69, 88, 130, 251, 262, 518
MseI TTAA 2 cut(s) 344, 401
MslI CAYNNNNRTG 1 cut(s) 411
MspR9I CCNGG 1 cut(s) 439
MunI CAATTG 2 cut(s) 106, 127
MvaI CCWGG 1 cut(s) 439
NcoI CCATGG 1 cut(s) 363
NdeII GATC 4 cut(s) 193, 253, 350, 474
NlaIII CATG 5 cut(s) 58, 260, 316, 367, 463
NlaIV GGNNCC 1 cut(s) 239
NspI RCATGY 1 cut(s) 316
PagI TCATGA 1 cut(s) 256
PciI ACATGT 1 cut(s) 312
PfeI GAWTC 4 cut(s) 260, 434, 501, 507
PkrI GCNGC 1 cut(s) 206
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PscI ACATGT 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 437
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 1 cut(s) 239
PstI CTGCAG 1 cut(s) 268
PstNI CAGNNNCTG 2 cut(s) 80, 197
PsuI RGATCY 3 cut(s) 193, 350, 474
RsaI GTAC 2 cut(s) 38, 99
RsaNI GTAC 2 cut(s) 37, 98
RseI CAYNNNNRTG 1 cut(s) 411
SaqAI TTAA 2 cut(s) 344, 401
SatI GCNGC 1 cut(s) 205
Sau3AI GATC 4 cut(s) 193, 253, 350, 474
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 439
SduI GDGCHC 1 cut(s) 175
SetI ASST 5 cut(s) 99, 153, 273, 429, 449
SfaNI GCATC 2 cut(s) 61, 130
SfcI CTRYAG 2 cut(s) 264, 478
SmiMI CAYNNNNRTG 1 cut(s) 411
Sse9I AATT 6 cut(s) 45, 106, 127, 389, 467, 486
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 437
StyI CCWWGG 1 cut(s) 363
TasI AATT 6 cut(s) 45, 106, 127, 389, 467, 486
TfiI GAWTC 4 cut(s) 260, 434, 501, 507
Tru1I TTAA 2 cut(s) 344, 401
Tru9I TTAA 2 cut(s) 344, 401
TseI GCWGC 1 cut(s) 204
TspDTI ATGAA 4 cut(s) 39, 234, 367, 399
XapI RAATTY 1 cut(s) 467
XceI RCATGY 1 cut(s) 316
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.