Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp01

Orthogroup ID

OG0002862

Physical Location & Seq

Genomic Coordinates Forward (+)

37919695 .. 37923512

Gene Structure 3818 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.1G449300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1668 bp

ATGGCATTCTCTTCTTATGCTAATTTACAAGTGCACGGGTCTTTCCAAAACCCTTTCATCTTCCACGGCTTAAATCACCTCAAAACCTCACTTTCCTCGTCTGTTAAGCCTCTATTCAAAGAGTTGCAGCAATTGCTTCCAAGGAAGGTCGATGTTTCTAAAACCATGAAAAACACTTCTGGGAAGATGTTAGATGCACTTGTGGACTCTATTTTCCAGTTTGTAGATCAACCGTTACTCCCATCTCAGAAAAACTTTGCACCAGTGGAAGAGATTGGAGACCATGTTGAGGTTACCTGGACCGAAGGAGAAATTCCCGCTGATTTTGCTGAAGGTGTTTTCATAAGAAATGGCATAAATCCTTTATTCGGAGGTTTAAAATCAGCAGTGTCGATTTTCAGACAAACACAGCACACTTGGGTAGAGGGAGAAGGCATGCTTCATGCACTCTACTTCAAAAAAGATCATGATGGAAGTTGGATTATCTCCTACAACAACAGATATGTTGAGACTGAAACATTTAAGCTAGAGAAACAGAAGCATAATAAGCCATGTTTCTTACCAGCCCTAGAAGGAGATGCAGCAGCAGTTGTAGCAGCATATATTCTAAATGGGTTGCGGTTTGGCACAATCAATAAACACTTGAGCAACACCAATAAAGCTCCAAACTCCGGAAAGCTAGTCATAATGGGGGTAGATGCAAAGAAACCTTACTATGTTTTGGGTGTTATCTCTGCTGATGGAAAATTACTGCATAAGGTTGATCTCGAGTTCAAAAGAAGTACGCTTAGCCATGATATAGGAGTCACACAGAAGTACAATGTAATCATTGATCATCCCCTCACGGTTGATGTAAAGAGAGTTACCATGGGTGGCCCATTATTGAAGTATGAAAAGGAAGAGTATGCAAGGATTGGAGTGATGCCACGTTATGGTGATGCAGAGTCAGTCAAGTGGTTTGAGGTACAAACCAATTGCACATTTCACATTCTCAATTGCTTTGAGGAGGCTAATGAGGTTGTAGTGAGGGGATGCAGGGCTCTCACATCCTTCTTGCCAGGTCCTGATGGTGTACTTAACAAATATGAGTGGTTCTCAAAAGGGTTCAACTTTGCAGATGATCATTCTGCAGAGACCGGATATTTGTTTTCTCGTTTATATGAATGGAGATTGAACATGGTCTCTGGAAATGTGGAGGAGAGAAATTTAACCGGAACTGATTTCTCCATGGATTTTCCTTTCATCAATGAACAAGTAACTGGTTTGAAACACAAGTATGGCTATACACAGGTCATTGATTCTATGGCAAGCTCTGACACTGGCATGGGAAAATATGGATCTCTAGCCAAATTATATTTGGAGGAATCGAATTCTACGTCATTTGTGGAGGGAAAGTGTGAGGATTTGATAAAAGTTGAATACCATAAGTTTGAGGAGAAAAACTTTTGCAGTGGAAGCGTTTTTGTGGCTAGGCATGGAGGGAAAGGCATGGAGGAAGATGATGGTTGGATTGTCACTTTTGTCCACAATGAAGAAACTGATGTCACCCAAGTTCATGTAATTGACGCAAAGAAATTTGAAAGTGAACCGATTGCGAAACTCACATTGCCACAGCGGGTGCCATATGGTTTTCATGCAACTTTTGTGTCAATGCCTTGTCAATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RPE65

Protein Analysis

556

Amino Acids

62.49

Weight (kDa)

6.01

Isoelectric Point (pI)

26.47

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002862)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35571 FvH4_2g35680 FvH4_2g35681 FvH4_2g35682 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520 FvH4_5g00520
malus_domestica	MD15G1094300.v1.1
prunus_persica	Prupe.1G449200_v2.0.a1 Prupe.1G449300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0306121
rosa_laevigata	RLG00000010823 RLG00000016512
rosa_multiflora	Rmu_sc0000785.1_g000018 Rmu_sc0000785.1_g000021 Rmu_sc0003594.1_g000022
rosa_roxburghii	Rroxscaffold_7G00162040 Rroxscaffold_7G00177250
rosa_rugosa	Rorug06G0282200 Rorug06G0346800 Rorug06G0346800
rosa_samantha	Rh6AG459300 Rh6AG459400 Rh6BG441600 Rh6CG473800 Rh6DG460000
rosa_wichuraiana	Rw6G040040 Rw7G016580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1618
AccB7I	CCANNNNNTGG	1 cut(s)	932
AccIII	TCCGGA	1 cut(s)	671
AciI	CCGC	3 cut(s)	318, 619, 1615
AclWI	GGATC	1 cut(s)	1345
AcsI	RAATTY	4 cut(s)	312, 1204, 1369, 1574
AcuI	CTGAAG	1 cut(s)	351
AfaI	GTAC	4 cut(s)	784, 818, 966, 1074
AfiI	CCNNNNNNNGG	4 cut(s)	289, 368, 671, 932
AgsI	TTSAA	9 cut(s)	118, 457, 775, 886, 1108, 1174, 1267, 1418, 1580
AjnI	CCWGG	2 cut(s)	296, 1057
AluBI	AGCT	4 cut(s)	526, 662, 679, 1311
AluI	AGCT	4 cut(s)	526, 662, 679, 1311
Alw21I	GWGCWC	1 cut(s)	36
Alw26I	GTCTC	4 cut(s)	273, 503, 1127, 1186
Alw44I	GTGCAC	1 cut(s)	32
AlwI	GGATC	1 cut(s)	1345
AlwNI	CAGNNNCTG	1 cut(s)	1064
Ama87I	CYCGRG	1 cut(s)	767
Aor13HI	TCCGGA	1 cut(s)	671
AoxI	GGCC	1 cut(s)	874
ApaLI	GTGCAC	1 cut(s)	32
ApeKI	GCWGC	4 cut(s)	127, 581, 584, 596
ApoI	RAATTY	4 cut(s)	312, 1204, 1369, 1574
ArsI	GACNNNNNNTTYG	4 cut(s)	1126, 1158, 1361, 1393
AspS9I	GGNCC	3 cut(s)	300, 875, 1061
AsuHPI	GGTGA	3 cut(s)	68, 947, 1537
AvaI	CYCGRG	1 cut(s)	767
AvaII	GGWCC	2 cut(s)	300, 1061
BaeGI	GKGCMC	1 cut(s)	36
BanI	GGYRCC	1 cut(s)	1618
BanII	GRGCYC	1 cut(s)	1042
Bbv12I	GWGCWC	1 cut(s)	36
BbvI	GCAGC	4 cut(s)	139, 593, 596, 608
BccI	CCATC	5 cut(s)	250, 464, 734, 1061, 1496
BceAI	ACGGC	1 cut(s)	82
BciT130I	CCWGG	2 cut(s)	298, 1059
BclI	TGATCA	2 cut(s)	832, 1120
BcoDI	GTCTC	4 cut(s)	273, 503, 1127, 1186
BfaI	CTAG	5 cut(s)	527, 569, 680, 1343, 1470
BfmI	CTRYAG	1 cut(s)	1128
BisI	GCNGC	4 cut(s)	128, 582, 585, 597
BlpI	GCTNAGC	1 cut(s)	788
BlsI	GCNGC	4 cut(s)	129, 583, 586, 598
Bme1390I	CCNGG	2 cut(s)	298, 1059
Bme18I	GGWCC	2 cut(s)	300, 1061
BmeT110I	CYCGRG	1 cut(s)	767
BmgT120I	GGNCC	3 cut(s)	300, 875, 1061
BmiI	GGNNCC	1 cut(s)	1620
BmrFI	CCNGG	2 cut(s)	298, 1059
BmsI	GCATC	6 cut(s)	184, 568, 688, 912, 928, 1022
BplI	GAGNNNNNCTC	2 cut(s)	1079, 1111
Bpu1102I	GCTNAGC	1 cut(s)	788
BpuEI	CTTGAG	1 cut(s)	664
BsaI	GGTCTC	3 cut(s)	273, 1127, 1186
BsaJI	CCNNGG	4 cut(s)	64, 140, 867, 1227
BsaWI	WCCGGW	3 cut(s)	671, 1136, 1211
BsaXI	ACNNNNNCTCC	6 cut(s)	222, 252, 270, 300, 1379, 1409
Bsc4I	CCNNNNNNNGG	4 cut(s)	289, 368, 671, 932
Bse1I	ACTGG	4 cut(s)	217, 263, 1264, 1324
Bse3DI	GCAATG	1 cut(s)	1604
BseAI	TCCGGA	1 cut(s)	671
BseBI	CCWGG	2 cut(s)	298, 1059
BseDI	CCNNGG	4 cut(s)	64, 140, 867, 1227
BseGI	GGATG	3 cut(s)	835, 1037, 1046
BseLI	CCNNNNNNNGG	4 cut(s)	289, 368, 671, 932
BseMI	GCAATG	1 cut(s)	1604
BseMII	CTCAG	1 cut(s)	260
BseNI	ACTGG	4 cut(s)	217, 263, 1264, 1324
BseRI	GAGGAG	3 cut(s)	1019, 1211, 1448
BseSI	GKGCMC	1 cut(s)	36
BseXI	GCAGC	4 cut(s)	139, 593, 596, 608
BshFI	GGCC	1 cut(s)	876
BshNI	GGYRCC	1 cut(s)	1618
BsiHKAI	GWGCWC	1 cut(s)	36
BsiHKCI	CYCGRG	1 cut(s)	767
BsiSI	CCGG	3 cut(s)	672, 1137, 1212
BslI	CCNNNNNNNGG	4 cut(s)	289, 368, 671, 932
BsmAI	GTCTC	4 cut(s)	273, 503, 1127, 1186
BsmI	GAATGC	1 cut(s)	5
BsnI	GGCC	1 cut(s)	876
Bso31I	GGTCTC	3 cut(s)	273, 1127, 1186
BsoBI	CYCGRG	1 cut(s)	767
Bsp1286I	GDGCHC	2 cut(s)	36, 1042
Bsp13I	TCCGGA	1 cut(s)	671
Bsp143I	GATC	6 cut(s)	226, 463, 763, 832, 1120, 1337
Bsp1720I	GCTNAGC	1 cut(s)	788
Bsp19I	CCATGG	2 cut(s)	867, 1227
BspACI	CCGC	3 cut(s)	318, 619, 1615
BspANI	GGCC	1 cut(s)	876
BspCNI	CTCAG	1 cut(s)	259
BspEI	TCCGGA	1 cut(s)	671
BspHI	TCATGA	1 cut(s)	466
BspLI	GGNNCC	1 cut(s)	1620
BspMAI	CTGCAG	1 cut(s)	1132
BspPI	GGATC	1 cut(s)	1345
BspT107I	GGYRCC	1 cut(s)	1618
BspTNI	GGTCTC	3 cut(s)	273, 1127, 1186
BsrDI	GCAATG	1 cut(s)	1604
BsrI	ACTGG	4 cut(s)	217, 263, 1264, 1324
BssECI	CCNNGG	4 cut(s)	64, 140, 867, 1227
BssMI	GATC	6 cut(s)	226, 463, 763, 832, 1120, 1337
BssT1I	CCWWGG	3 cut(s)	140, 867, 1227
Bst2UI	CCWGG	2 cut(s)	298, 1059
Bst4CI	ACNGT	2 cut(s)	234, 847
Bst6I	CTCTTC	3 cut(s)	16, 264, 894
BstAPI	GCANNNNNTGC	1 cut(s)	133
BstC8I	GCNNGC	2 cut(s)	437, 1309
BstDEI	CTNAG	3 cut(s)	246, 788, 1665
BstDSI	CCRYGG	3 cut(s)	64, 867, 1227
BstEII	GGTNACC	1 cut(s)	292
BstF5I	GGATG	3 cut(s)	835, 1037, 1046
BstKTI	GATC	6 cut(s)	229, 466, 766, 835, 1123, 1340
BstMAI	GTCTC	4 cut(s)	273, 503, 1127, 1186
BstMBI	GATC	6 cut(s)	226, 463, 763, 832, 1120, 1337
BstMWI	GCNNNNNNNGC	5 cut(s)	133, 326, 547, 593, 1455
BstNI	CCWGG	2 cut(s)	298, 1059
BstNSI	RCATGY	1 cut(s)	439
BstPI	GGTNACC	1 cut(s)	292
BstSCI	CCNGG	2 cut(s)	296, 1057
BstSFI	CTRYAG	1 cut(s)	1128
BstSLI	GKGCMC	1 cut(s)	36
BstV1I	GCAGC	4 cut(s)	139, 593, 596, 608
BstX2I	RGATCY	1 cut(s)	1337
BstYI	RGATCY	1 cut(s)	1337
BsuRI	GGCC	1 cut(s)	876
BtgI	CCRYGG	3 cut(s)	64, 867, 1227
BtsCI	GGATG	3 cut(s)	835, 1037, 1046
BtsI	GCAGTG	2 cut(s)	393, 1456
BtsIMutI	CAGTG	4 cut(s)	270, 393, 1317, 1456
Cac8I	GCNNGC	2 cut(s)	437, 1309
CaiI	CAGNNNCTG	1 cut(s)	1064
CciI	TCATGA	1 cut(s)	466
Cfr13I	GGNCC	3 cut(s)	300, 875, 1061
CseI	GACGC	1 cut(s)	1574
Csp6I	GTAC	4 cut(s)	783, 817, 965, 1073
CviQI	GTAC	4 cut(s)	783, 817, 965, 1073
DdeI	CTNAG	3 cut(s)	246, 788, 1665
DpnI	GATC	6 cut(s)	228, 465, 765, 834, 1122, 1339
DpnII	GATC	6 cut(s)	226, 463, 763, 832, 1120, 1337
DraI	TTTAAA	1 cut(s)	378
Eam1104I	CTCTTC	3 cut(s)	16, 264, 894
EarI	CTCTTC	3 cut(s)	16, 264, 894
Eco130I	CCWWGG	3 cut(s)	140, 867, 1227
Eco24I	GRGCYC	1 cut(s)	1042
Eco31I	GGTCTC	3 cut(s)	273, 1127, 1186
Eco47I	GGWCC	2 cut(s)	300, 1061
Eco57I	CTGAAG	1 cut(s)	351
Eco88I	CYCGRG	1 cut(s)	767
Eco91I	GGTNACC	1 cut(s)	292
EcoO109I	RGGNCCY	1 cut(s)	1061
EcoO65I	GGTNACC	1 cut(s)	292
EcoRI	GAATTC	1 cut(s)	1369
EcoRII	CCWGG	2 cut(s)	296, 1057
EcoT14I	CCWWGG	3 cut(s)	140, 867, 1227
EcoT38I	GRGCYC	1 cut(s)	1042
ErhI	CCWWGG	3 cut(s)	140, 867, 1227
FalI	AAGNNNNNCTT	2 cut(s)	423, 455
FauI	CCCGC	2 cut(s)	325, 1608
FauNDI	CATATG	1 cut(s)	1624
FbaI	TGATCA	2 cut(s)	832, 1120
Fnu4HI	GCNGC	4 cut(s)	128, 582, 585, 597
FokI	GGATG	3 cut(s)	822, 1033, 1044
FriOI	GRGCYC	1 cut(s)	1042
Fsp4HI	GCNGC	4 cut(s)	128, 582, 585, 597
FspBI	CTAG	5 cut(s)	527, 569, 680, 1343, 1470
GluI	GCNGC	4 cut(s)	128, 582, 585, 597
HaeIII	GGCC	1 cut(s)	876
HapII	CCGG	3 cut(s)	672, 1137, 1212
HgaI	GACGC	1 cut(s)	1574
HinfI	GANTC	5 cut(s)	206, 804, 944, 1298, 1364
HpaII	CCGG	3 cut(s)	672, 1137, 1212
HphI	GGTGA	3 cut(s)	68, 947, 1537
Hpy166II	GTNNAC	5 cut(s)	34, 205, 1073, 1525, 1586
Hpy188I	TCNGA	4 cut(s)	249, 371, 401, 1315
Hpy188III	TCNNGA	5 cut(s)	467, 672, 767, 1064, 1185
Hpy8I	GTNNAC	5 cut(s)	34, 205, 1073, 1525, 1586
HpyAV	CCTTC	6 cut(s)	139, 299, 326, 425, 566, 1060
HpyCH4III	ACNGT	2 cut(s)	234, 847
HpyCH4IV	ACGT	2 cut(s)	928, 1376
HpyF10VI	GCNNNNNNNGC	5 cut(s)	133, 326, 547, 593, 1455
HpyF3I	CTNAG	3 cut(s)	246, 788, 1665
HpySE526I	ACGT	2 cut(s)	928, 1376
Kpn2I	TCCGGA	1 cut(s)	671
Ksp22I	TGATCA	2 cut(s)	832, 1120
Kzo9I	GATC	6 cut(s)	226, 463, 763, 832, 1120, 1337
LmnI	GCTCC	1 cut(s)	667
Lsp1109I	GCAGC	4 cut(s)	139, 593, 596, 608
LweI	GCATC	6 cut(s)	184, 568, 688, 912, 928, 1022
MaeI	CTAG	5 cut(s)	527, 569, 680, 1343, 1470
MaeII	ACGT	2 cut(s)	928, 1376
MaeIII	GTNAC	7 cut(s)	234, 292, 805, 862, 1255, 1513, 1543
MalI	GATC	6 cut(s)	228, 465, 765, 834, 1122, 1339
MboI	GATC	6 cut(s)	226, 463, 763, 832, 1120, 1337
MboII	GAAGA	7 cut(s)	3, 52, 196, 281, 911, 1508, 1544
MfeI	CAATTG	3 cut(s)	131, 973, 994
MflI	RGATCY	1 cut(s)	1337
MhlI	GDGCHC	2 cut(s)	36, 1042
MlyI	GAGTC	3 cut(s)	200, 813, 953
MmeI	TCCRAC	2 cut(s)	458, 1487
MroI	TCCGGA	1 cut(s)	671
MseI	TTAA	6 cut(s)	71, 105, 377, 522, 1077, 1208
MslI	CAYNNNNRTG	2 cut(s)	1275, 1322
MspA1I	CMGCKG	2 cut(s)	320, 1615
MspI	CCGG	3 cut(s)	672, 1137, 1212
MspR9I	CCNGG	2 cut(s)	298, 1059
MunI	CAATTG	3 cut(s)	131, 973, 994
Mva1269I	GAATGC	1 cut(s)	5
MvaI	CCWGG	2 cut(s)	298, 1059
MwoI	GCNNNNNNNGC	5 cut(s)	133, 326, 547, 593, 1455
NcoI	CCATGG	2 cut(s)	867, 1227
NdeI	CATATG	1 cut(s)	1624
NdeII	GATC	6 cut(s)	226, 463, 763, 832, 1120, 1337
NlaIV	GGNNCC	1 cut(s)	1620
NmuCI	GTSAC	3 cut(s)	805, 1513, 1543
NspI	RCATGY	1 cut(s)	439
PaeI	GCATGC	1 cut(s)	439
PaeR7I	CTCGAG	1 cut(s)	767
PagI	TCATGA	1 cut(s)	466
PcsI	WCGNNNNNNNCGW	1 cut(s)	1373
PctI	GAATGC	1 cut(s)	5
PfeI	GAWTC	2 cut(s)	1298, 1364
PflMI	CCANNNNNTGG	1 cut(s)	932
PkrI	GCNGC	4 cut(s)	129, 583, 586, 598
PleI	GAGTC	3 cut(s)	200, 812, 952
PpsI	GAGTC	3 cut(s)	200, 812, 952
PpuMI	RGGWCCY	1 cut(s)	1061
Psp5II	RGGWCCY	1 cut(s)	1061
Psp6I	CCWGG	2 cut(s)	296, 1057
PspEI	GGTNACC	1 cut(s)	292
PspGI	CCWGG	2 cut(s)	296, 1057
PspN4I	GGNNCC	1 cut(s)	1620
PspPI	GGNCC	3 cut(s)	300, 875, 1061
PspPPI	RGGWCCY	1 cut(s)	1061
PstI	CTGCAG	1 cut(s)	1132
PstNI	CAGNNNCTG	1 cut(s)	1064
PsuI	RGATCY	1 cut(s)	1337
RsaI	GTAC	4 cut(s)	784, 818, 966, 1074
RsaNI	GTAC	4 cut(s)	783, 817, 965, 1073
RseI	CAYNNNNRTG	2 cut(s)	1275, 1322
SaqAI	TTAA	6 cut(s)	71, 105, 377, 522, 1077, 1208
SatI	GCNGC	4 cut(s)	128, 582, 585, 597
Sau3AI	GATC	6 cut(s)	226, 463, 763, 832, 1120, 1337
Sau96I	GGNCC	3 cut(s)	300, 875, 1061
SchI	GAGTC	3 cut(s)	200, 813, 953
ScrFI	CCNGG	2 cut(s)	298, 1059
SduI	GDGCHC	2 cut(s)	36, 1042
SfaNI	GCATC	6 cut(s)	184, 568, 688, 912, 928, 1022
SfcI	CTRYAG	1 cut(s)	1128
Sfr274I	CTCGAG	1 cut(s)	767
SinI	GGWCC	2 cut(s)	300, 1061
SlaI	CTCGAG	1 cut(s)	767
SmiMI	CAYNNNNRTG	2 cut(s)	1275, 1322
SmlI	CTYRAG	2 cut(s)	643, 767
SmoI	CTYRAG	2 cut(s)	643, 767
SphI	GCATGC	1 cut(s)	439
SsiI	CCGC	3 cut(s)	318, 619, 1615
SspMI	CTAG	5 cut(s)	527, 569, 680, 1343, 1470
StyD4I	CCNGG	2 cut(s)	296, 1057
StyI	CCWWGG	3 cut(s)	140, 867, 1227
TaaI	ACNGT	2 cut(s)	234, 847
TaiI	ACGT	2 cut(s)	931, 1379
TaqI	TCGA	4 cut(s)	150, 392, 768, 1367
TaqII	GACCGA	1 cut(s)	317
TatI	WGTACW	2 cut(s)	816, 1072
TfiI	GAWTC	2 cut(s)	1298, 1364
Tru1I	TTAA	6 cut(s)	71, 105, 377, 522, 1077, 1208
Tru9I	TTAA	6 cut(s)	71, 105, 377, 522, 1077, 1208
TscAI	CASTG	4 cut(s)	270, 393, 1324, 1456
TseFI	GTSAC	3 cut(s)	805, 1513, 1543
TseI	GCWGC	4 cut(s)	127, 581, 584, 596
Tsp45I	GTSAC	3 cut(s)	805, 1513, 1543
TspRI	CASTG	4 cut(s)	270, 393, 1324, 1456
Van91I	CCANNNNNTGG	1 cut(s)	932
VneI	GTGCAC	1 cut(s)	32
VpaK11BI	GGWCC	2 cut(s)	300, 1061
XapI	RAATTY	4 cut(s)	312, 1204, 1369, 1574
XceI	RCATGY	1 cut(s)	439
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1354
XhoI	CTCGAG	1 cut(s)	767
XspI	CTAG	5 cut(s)	527, 569, 680, 1343, 1470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.