FvH4_5g00520

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002862
Physical Location & Seq
Forward (+)
330013 .. 334935
4923 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00520.t2

Sequence Viewer

Length: 1794 bp
ATGGCAATCTCTTCGTATAATGCTTTACAAGTGAGCGGCGCATTGCCAAACCGTCTCATTTCCCACAGCTTTGATCATCTCAAAACCTCACTTTCCTCGACTGTTCAGCCTTTCTTCAAAGAGTTGCACCAATTGCTTCCCAGGAAGGTGGATGTCCCGAACACCATGAAAAACACTTCGGGGAAAATGTTAGATGCAGTTGTGGACTCTCTGTTCCAGTTTACGGATGTACCCTCACTCCCATCTCAGAAAAACTTTTTACCCGTTGAAGAGATTGGAGAACATGTTGAGGTCGCCTGCAACTTGGGAGAAATTCCTGCTGATTTCACTGAGGGTGTTTTCATCAGAAATGGCAGTAATCCTTTGTTTGGAGGTCTAAAAGCAGCAGTTTCGATCTTCGGAGAATCACACAATATTTGGGTAGAGGGAGAAGGAATGCTCCACGCTGTATACTTCAAGAAGGATGATGACAGTGGAAGTTGGATCATCTCATATAAGAATAGATATGTTGAGACTGAGACATTTAAGAACGAAACTCAACGAAATAAACCAGCTTTCTTACCAGCCATAGAAGGAGACGCTTCAGCAGTTACAGCAGCATTTATCCTAAATACGTTGAGATTTGGGGAAGTCATTAAACACATGAGCAACACCAATGTTTTTGAGCATTCAGGCAGATTTTACTCGATTGCAGAGAACTGTCTACCTCAAGAGGTGGACATACTGACCTTGGAAACCGGTTGTGATTGGGATGTCAATGGAACTTGGGATCGACCTTTCACAAGTCATCCAAAGAAAGCTCCATACTCCGGAGAGTTAGTCATGATGGGGACAGATGCATGGAAACCTTACTATGTTTTGGGGGTCATCTCCGCTGATGGAAAGAAAGTGCACAAGGTTGATCTCAAATTCAAAAGAAGTTCGTTCAGTCATGATATAGGAGTCACGGAAAAGTACAATGTAATCCTTGATCTTCCCCTCACATTGGATGTAAAGAGGCTCACCCTGGGAGGCCAAATAATGAAGTATGTAAAGGAAGAGTACGCTAGAATTGGGGTGATGCCTCGTTATGGAGATGCAGAGTCTGTCAAGTGGTTTGAGGTACAGGCCAATAGCACACTTCACTTTCTCAATTGCTTTGAGGATGATAATCAGGTTATAGTGAGGGGATGTAGGGCTCTCGAATCCGTTATATCAGATCCTGACGGCAGCCTAAACCCTTACGAATGGTTCCATAAAGGGTTCAAGTTTTCTGGTGATGATCATGATTCTGCAGAGGTTGGATATCTCTTCACGCATTTATATGAATGGAGATTGAACATGTTAACTGGGGAAGTCGAGGAGAGAAATTTAACTGGAAATGAATACTCCATGGATTTCCCTTTCATCAACGAACACTTTACTGGTTTAAAACACAAGTATGGATATACACAAGTCATTGATTCCGGGGCAAGCTCTTTATCTGGCATGGCTAAATTTGGATCTCTAGCCAAATTATACTTGGAGGAATCAAATCCTACTTATCAGGAGGGAAGGATAAAGGTAGAATACCATAAGTTTGAGGAAAACAACTTTTGCAGTGGAAGTGTTTTTGTCCCCAGAAATGGAGGCATCAAGGAAGATGATGGCTGGATTATCAGTTTTGTCCACAATGAAGAAACTGATGAGACCCAAGTTCATATCATTGATGCACAGAATTTTGAGGGTGAAGCGATTGCAAAAATTACATTGCCACAACGGGTGCCCTATGGTTTTCATGGAAACTTTTTTCCAATGCCAGCTCGTCGATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

598

Amino Acids

67.35

Weight (kDa)

5.34

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 89 - 591 2e-111 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1741
AccBSI CCGCTC 1 cut(s) 36
AccI GTMKAC 2 cut(s) 450, 703
AccIII TCCGGA 1 cut(s) 809
AciI CCGC 2 cut(s) 36, 873
AclWI GGATC 4 cut(s) 491, 777, 1193, 1489
AcsI RAATTY 5 cut(s) 312, 908, 1348, 1475, 1696
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 4 cut(s) 231, 956, 1043, 1104
AfiI CCNNNNNNNGG 6 cut(s) 223, 368, 809, 985, 1070, 1604
AflIII ACRYGT 2 cut(s) 283, 1320
AgeI ACCGGT 1 cut(s) 737
AgsI TTSAA 6 cut(s) 118, 269, 457, 913, 1246, 1318
AjnI CCWGG 2 cut(s) 140, 1005
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 5 cut(s) 69, 554, 800, 1455, 1781
AluI AGCT 5 cut(s) 69, 554, 800, 1455, 1781
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 5 cut(s) 59, 506, 512, 570, 1661
Alw44I GTGCAC 1 cut(s) 890
AlwI GGATC 4 cut(s) 491, 777, 1193, 1489
AlwNI CAGNNNCTG 2 cut(s) 1085, 1202
Aor13HI TCCGGA 1 cut(s) 809
AoxI GGCC 2 cut(s) 1012, 1107
ApaLI GTGCAC 1 cut(s) 890
ApeKI GCWGC 3 cut(s) 383, 596, 1209
ApoI RAATTY 5 cut(s) 312, 908, 1348, 1475, 1696
AsiGI ACCGGT 1 cut(s) 737
AspLEI GCGC 1 cut(s) 41
AsuC2I CCSGG 1 cut(s) 1447
AsuHPI GGTGA 4 cut(s) 994, 1069, 1268, 1718
BaeGI GKGCMC 2 cut(s) 894, 1746
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BanI GGYRCC 1 cut(s) 1741
BanII GRGCYC 1 cut(s) 1180
Bbv12I GWGCWC 1 cut(s) 894
BbvI GCAGC 3 cut(s) 395, 608, 1221
BccI CCATC 4 cut(s) 250, 820, 872, 1619
BceAI ACGGC 1 cut(s) 1222
BcgI CGANNNNNNTGC 3 cut(s) 28, 372, 406
BciT130I CCWGG 2 cut(s) 142, 1007
BclI TGATCA 2 cut(s) 73, 1261
BcnI CCSGG 1 cut(s) 1447
BcoDI GTCTC 5 cut(s) 59, 506, 512, 570, 1661
BfaI CTAG 2 cut(s) 1047, 1487
BfmI CTRYAG 1 cut(s) 1272
BisI GCNGC 4 cut(s) 37, 384, 597, 1210
BlsI GCNGC 4 cut(s) 38, 385, 598, 1211
Bme1390I CCNGG 3 cut(s) 142, 1007, 1447
BmiI GGNNCC 2 cut(s) 1232, 1743
BmrFI CCNGG 3 cut(s) 142, 1007, 1447
BmrI ACTGGG 1 cut(s) 1338
BmsI GCATC 6 cut(s) 184, 826, 1050, 1066, 1620, 1678
BmuI ACTGGG 1 cut(s) 1338
BpuEI CTTGAG 1 cut(s) 693
BpuMI CCSGG 1 cut(s) 1447
BsaBI GATNNNNATC 1 cut(s) 1149
BsaI GGTCTC 1 cut(s) 1661
BsaJI CCNNGG 6 cut(s) 140, 729, 1005, 1006, 1371, 1446
BsaWI WCCGGW 2 cut(s) 737, 809
BsaXI ACNNNNNCTCC 6 cut(s) 222, 252, 270, 300, 804, 834
Bsc4I CCNNNNNNNGG 6 cut(s) 223, 368, 809, 985, 1070, 1604
Bse118I RCCGGY 1 cut(s) 737
Bse1I ACTGG 4 cut(s) 217, 1333, 1360, 1408
Bse3DI GCAATG 2 cut(s) 41, 1727
Bse8I GATNNNNATC 1 cut(s) 1149
BseAI TCCGGA 1 cut(s) 809
BseBI CCWGG 2 cut(s) 142, 1007
BseDI CCNNGG 6 cut(s) 140, 729, 1005, 1006, 1371, 1446
BseGI GGATG 8 cut(s) 157, 232, 469, 757, 787, 994, 1150, 1175
BseJI GATNNNNATC 1 cut(s) 1149
BseLI CCNNNNNNNGG 6 cut(s) 223, 368, 809, 985, 1070, 1604
BseMI GCAATG 2 cut(s) 41, 1727
BseMII CTCAG 3 cut(s) 260, 321, 507
BseNI ACTGG 4 cut(s) 217, 1333, 1360, 1408
BseRI GAGGAG 1 cut(s) 1355
BseSI GKGCMC 2 cut(s) 894, 1746
BseXI GCAGC 3 cut(s) 395, 608, 1221
BshFI GGCC 2 cut(s) 1014, 1109
BshNI GGYRCC 1 cut(s) 1741
BshTI ACCGGT 1 cut(s) 737
BsiHKAI GWGCWC 1 cut(s) 894
BsiSI CCGG 3 cut(s) 738, 810, 1446
BslFI GGGAC 3 cut(s) 140, 844, 1580
BslI CCNNNNNNNGG 6 cut(s) 223, 368, 809, 985, 1070, 1604
BsmAI GTCTC 5 cut(s) 59, 506, 512, 570, 1661
BsmBI CGTCTC 2 cut(s) 59, 570
BsmFI GGGAC 3 cut(s) 140, 844, 1580
BsmI GAATGC 2 cut(s) 441, 667
BsnI GGCC 2 cut(s) 1014, 1109
Bso31I GGTCTC 1 cut(s) 1661
Bsp1286I GDGCHC 3 cut(s) 894, 1180, 1746
Bsp13I TCCGGA 1 cut(s) 809
Bsp19I CCATGG 1 cut(s) 1371
BspACI CCGC 2 cut(s) 36, 873
BspANI GGCC 2 cut(s) 1014, 1109
BspCNI CTCAG 3 cut(s) 259, 322, 508
BspEI TCCGGA 1 cut(s) 809
BspHI TCATGA 3 cut(s) 822, 931, 1264
BspLI GGNNCC 2 cut(s) 1232, 1743
BspMAI CTGCAG 1 cut(s) 1276
BspPI GGATC 4 cut(s) 491, 777, 1193, 1489
BspT107I GGYRCC 1 cut(s) 1741
BspTNI GGTCTC 1 cut(s) 1661
BsrBI CCGCTC 1 cut(s) 36
BsrDI GCAATG 2 cut(s) 41, 1727
BsrFI RCCGGY 1 cut(s) 737
BsrI ACTGG 4 cut(s) 217, 1333, 1360, 1408
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 6 cut(s) 140, 729, 1005, 1006, 1371, 1446
BssNAI GTATAC 1 cut(s) 451
BssT1I CCWWGG 2 cut(s) 729, 1371
Bst1107I GTATAC 1 cut(s) 451
Bst2UI CCWGG 2 cut(s) 142, 1007
Bst4CI ACNGT 4 cut(s) 53, 103, 473, 701
Bst6I CTCTTC 4 cut(s) 16, 264, 1032, 1295
BstAPI GCANNNNNTGC 1 cut(s) 133
BstC8I GCNNGC 3 cut(s) 298, 1453, 1779
BstDEI CTNAG 4 cut(s) 246, 330, 516, 1791
BstDSI CCRYGG 1 cut(s) 1371
BstF5I GGATG 8 cut(s) 157, 232, 469, 757, 787, 994, 1150, 1175
BstHHI GCGC 1 cut(s) 41
BstMAI GTCTC 5 cut(s) 59, 506, 512, 570, 1661
BstMWI GCNNNNNNNGC 2 cut(s) 133, 593
BstNI CCWGG 2 cut(s) 142, 1007
BstNSI RCATGY 2 cut(s) 287, 1324
BstSCI CCNGG 3 cut(s) 140, 1005, 1445
BstSFI CTRYAG 1 cut(s) 1272
BstSLI GKGCMC 2 cut(s) 894, 1746
BstV1I GCAGC 3 cut(s) 395, 608, 1221
BstX2I RGATCY 2 cut(s) 1198, 1481
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 2 cut(s) 1198, 1481
BstZ17I GTATAC 1 cut(s) 451
BsuRI GGCC 2 cut(s) 1014, 1109
BtgI CCRYGG 1 cut(s) 1371
BtsCI GGATG 8 cut(s) 157, 232, 469, 757, 787, 994, 1150, 1175
BtsI GCAGTG 1 cut(s) 1585
BtsIMutI CAGTG 3 cut(s) 327, 478, 1585
Cac8I GCNNGC 3 cut(s) 298, 1453, 1779
CaiI CAGNNNCTG 2 cut(s) 1085, 1202
CciI TCATGA 3 cut(s) 822, 931, 1264
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 737
CseI GACGC 1 cut(s) 587
Csp6I GTAC 4 cut(s) 230, 955, 1042, 1103
CspAI ACCGGT 1 cut(s) 737
CviQI GTAC 4 cut(s) 230, 955, 1042, 1103
DdeI CTNAG 4 cut(s) 246, 330, 516, 1791
DraI TTTAAA 1 cut(s) 1410
Eam1104I CTCTTC 4 cut(s) 16, 264, 1032, 1295
EarI CTCTTC 4 cut(s) 16, 264, 1032, 1295
Eco130I CCWWGG 2 cut(s) 729, 1371
Eco24I GRGCYC 1 cut(s) 1180
Eco31I GGTCTC 1 cut(s) 1661
Eco32I GATATC 1 cut(s) 1286
Eco57I CTGAAG 1 cut(s) 567
EcoRII CCWGG 2 cut(s) 140, 1005
EcoRV GATATC 1 cut(s) 1286
EcoT14I CCWWGG 2 cut(s) 729, 1371
EcoT22I ATGCAT 1 cut(s) 841
EcoT38I GRGCYC 1 cut(s) 1180
ErhI CCWWGG 2 cut(s) 729, 1371
Esp3I CGTCTC 2 cut(s) 59, 570
FaqI GGGAC 3 cut(s) 140, 844, 1580
FbaI TGATCA 2 cut(s) 73, 1261
FblI GTMKAC 2 cut(s) 450, 703
Fnu4HI GCNGC 4 cut(s) 37, 384, 597, 1210
FokI GGATG 8 cut(s) 164, 239, 476, 764, 774, 1001, 1157, 1182
FriOI GRGCYC 1 cut(s) 1180
Fsp4HI GCNGC 4 cut(s) 37, 384, 597, 1210
FspBI CTAG 2 cut(s) 1047, 1487
GlaI GCGC 1 cut(s) 40
GluI GCNGC 4 cut(s) 37, 384, 597, 1210
HaeIII GGCC 2 cut(s) 1014, 1109
HapII CCGG 3 cut(s) 738, 810, 1446
HgaI GACGC 1 cut(s) 587
HhaI GCGC 1 cut(s) 41
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HincII GTYRAC 1 cut(s) 1326
HindII GTYRAC 1 cut(s) 1326
HinfI GANTC 8 cut(s) 206, 404, 942, 1082, 1184, 1268, 1442, 1508
HpaI GTTAAC 1 cut(s) 1326
HpaII CCGG 3 cut(s) 738, 810, 1446
HphI GGTGA 4 cut(s) 994, 1069, 1268, 1718
Hpy166II GTNNAC 8 cut(s) 205, 222, 451, 704, 718, 892, 1326, 1648
Hpy188I TCNGA 4 cut(s) 249, 347, 401, 1198
Hpy8I GTNNAC 8 cut(s) 205, 222, 451, 704, 718, 892, 1326, 1648
Hpy99I CGWCG 1 cut(s) 1788
HpyAV CCTTC 5 cut(s) 139, 425, 454, 566, 1527
HpyCH4III ACNGT 4 cut(s) 53, 103, 473, 701
HpyCH4IV ACGT 1 cut(s) 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 593
HpyF3I CTNAG 4 cut(s) 246, 330, 516, 1791
HpySE526I ACGT 1 cut(s) 614
HspAI GCGC 1 cut(s) 39
Kpn2I TCCGGA 1 cut(s) 809
Ksp22I TGATCA 2 cut(s) 73, 1261
KspAI GTTAAC 1 cut(s) 1326
LmnI GCTCC 2 cut(s) 444, 805
Lsp1109I GCAGC 3 cut(s) 395, 608, 1221
LweI GCATC 6 cut(s) 184, 826, 1050, 1066, 1620, 1678
MaeI CTAG 2 cut(s) 1047, 1487
MaeII ACGT 1 cut(s) 614
MaeIII GTNAC 2 cut(s) 589, 943
MbiI CCGCTC 1 cut(s) 36
MboII GAAGA 9 cut(s) 3, 106, 281, 388, 965, 1049, 1282, 1631, 1667
MfeI CAATTG 2 cut(s) 131, 1132
MflI RGATCY 2 cut(s) 1198, 1481
MhlI GDGCHC 3 cut(s) 894, 1180, 1746
MlyI GAGTC 3 cut(s) 200, 951, 1091
MmeI TCCRAC 2 cut(s) 461, 1261
Mph1103I ATGCAT 1 cut(s) 841
MroI TCCGGA 1 cut(s) 809
MseI TTAA 5 cut(s) 525, 636, 1325, 1352, 1409
MslI CAYNNNNRTG 2 cut(s) 1302, 1419
MspA1I CMGCKG 1 cut(s) 875
MspI CCGG 3 cut(s) 738, 810, 1446
MspR9I CCNGG 3 cut(s) 142, 1007, 1447
MunI CAATTG 2 cut(s) 131, 1132
Mva1269I GAATGC 2 cut(s) 441, 667
MvaI CCWGG 2 cut(s) 142, 1007
MwoI GCNNNNNNNGC 2 cut(s) 133, 593
NciI CCSGG 1 cut(s) 1447
NcoI CCATGG 1 cut(s) 1371
NlaIV GGNNCC 2 cut(s) 1232, 1743
NmuCI GTSAC 1 cut(s) 943
NsiI ATGCAT 1 cut(s) 841
NspI RCATGY 2 cut(s) 287, 1324
PagI TCATGA 3 cut(s) 822, 931, 1264
PasI CCCWGGG 1 cut(s) 1006
PciI ACATGT 2 cut(s) 283, 1320
PctI GAATGC 2 cut(s) 441, 667
PfeI GAWTC 5 cut(s) 404, 1184, 1268, 1442, 1508
PinAI ACCGGT 1 cut(s) 737
PkrI GCNGC 4 cut(s) 38, 385, 598, 1211
PleI GAGTC 3 cut(s) 200, 950, 1090
PpsI GAGTC 3 cut(s) 200, 950, 1090
PscI ACATGT 2 cut(s) 283, 1320
Psp6I CCWGG 2 cut(s) 140, 1005
PspGI CCWGG 2 cut(s) 140, 1005
PspN4I GGNNCC 2 cut(s) 1232, 1743
PstI CTGCAG 1 cut(s) 1276
PstNI CAGNNNCTG 2 cut(s) 1085, 1202
PsuI RGATCY 2 cut(s) 1198, 1481
RsaI GTAC 4 cut(s) 231, 956, 1043, 1104
RsaNI GTAC 4 cut(s) 230, 955, 1042, 1103
RseI CAYNNNNRTG 2 cut(s) 1302, 1419
SaqAI TTAA 5 cut(s) 525, 636, 1325, 1352, 1409
SatI GCNGC 4 cut(s) 37, 384, 597, 1210
SchI GAGTC 3 cut(s) 200, 951, 1091
ScrFI CCNGG 3 cut(s) 142, 1007, 1447
SduI GDGCHC 3 cut(s) 894, 1180, 1746
SfaNI GCATC 6 cut(s) 184, 826, 1050, 1066, 1620, 1678
SfcI CTRYAG 1 cut(s) 1272
SmiMI CAYNNNNRTG 2 cut(s) 1302, 1419
SmlI CTYRAG 1 cut(s) 708
SmoI CTYRAG 1 cut(s) 708
SsiI CCGC 2 cut(s) 36, 873
SspI AATATT 1 cut(s) 415
SspMI CTAG 2 cut(s) 1047, 1487
StyD4I CCNGG 3 cut(s) 140, 1005, 1445
StyI CCWWGG 2 cut(s) 729, 1371
TaaI ACNGT 4 cut(s) 53, 103, 473, 701
TaiI ACGT 1 cut(s) 617
TaqI TCGA 7 cut(s) 98, 392, 686, 772, 1182, 1338, 1786
TatI WGTACW 1 cut(s) 954
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 5 cut(s) 404, 1184, 1268, 1442, 1508
Tru1I TTAA 5 cut(s) 525, 636, 1325, 1352, 1409
Tru9I TTAA 5 cut(s) 525, 636, 1325, 1352, 1409
TscAI CASTG 3 cut(s) 334, 478, 1585
TseFI GTSAC 1 cut(s) 943
TseI GCWGC 3 cut(s) 383, 596, 1209
Tsp45I GTSAC 1 cut(s) 943
TspDTI ATGAA 9 cut(s) 182, 331, 1037, 1320, 1375, 1377, 1667, 1668, 1745
TspGWI ACGGA 3 cut(s) 239, 962, 1177
TspRI CASTG 3 cut(s) 334, 478, 1585
VneI GTGCAC 1 cut(s) 890
XapI RAATTY 5 cut(s) 312, 908, 1348, 1475, 1696
XceI RCATGY 2 cut(s) 287, 1324
XcmI CCANNNNNNNNNTGG 1 cut(s) 1498
XmiI GTMKAC 2 cut(s) 450, 703
XspI CTAG 2 cut(s) 1047, 1487
Zsp2I ATGCAT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.