FvH4_2g35681

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002862
Physical Location & Seq
Forward (+)
26314907 .. 26316751
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35681.t1

Sequence Viewer

Length: 717 bp
ATGGCAATCTCTTCTTATAATGCCTTACATGTAAACGGGTCAGTTCAAAGCCGACTCAGCTCCCACGGCTTCAATCATCTTAAAACCTCTCTTTCTTCAACAGTCAAGCCTTTCTTCAAAGAGTTCCAGCTGCAATTGCTTCCGAGGAAGGTGGATGTCTTAAAAACCATGAAAAACACTTCGGGGAAATTGTTGGAAGCAGTTGTGGACTCCCTATTCCAGTTTGTTGATGTACCGTCACTCCCATCTCAGAAAAACTTTTTACCAGTTGAAGAAATCGGAGAACTTGTTGAGGTTACCTGCACTTCAGGAGAAATTCCTGCTGATTTTGCTGAGGGTGTCTTCATCAGAAATGGTGGTAATCCTTTGTTTGGAGGTCTGAAATCAGCAGTGTCGATCTTTGGAGAATCACACCATACTTGGGTAGAGGGAGAAGGAATGCTCCATGCCCTATACTTCAAAAGAGACGATGACCGTGATAGCTGGGTGATCTCGTATAAGAATAGATATGTTGAGACTGAGACATTCAAGATAGAAACTCAACTAAATAAACCAGGCTTCTTACCTGCCACAGCAGGAGACAGTCCAGCAACCATTGCAGCAATTCTCCTAAATTCGTTGAGATTTGGGACCGTCAATAAACGCACGAGCAACACCAGTGTCTTTGAGCATTCAGGGAAGTTTTACGCGAATGATGAGACCTATGTGCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

239

Amino Acids

26.37

Weight (kDa)

6.17

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 90 - 237 1.6e-23 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 18
Acc36I ACCTGC 2 cut(s) 308, 574
AccII CGCG 1 cut(s) 689
AcsI RAATTY 2 cut(s) 315, 613
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 2 cut(s) 371, 421
AflIII ACRYGT 1 cut(s) 28
AgsI TTSAA 7 cut(s) 47, 73, 99, 118, 272, 460, 529
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 3 cut(s) 60, 130, 483
AluI AGCT 3 cut(s) 60, 130, 483
Alw26I GTCTC 5 cut(s) 459, 509, 515, 573, 692
ApeKI GCWGC 2 cut(s) 130, 599
ApoI RAATTY 2 cut(s) 315, 613
AspS9I GGNCC 1 cut(s) 630
AsuHPI GGTGA 1 cut(s) 499
AvaII GGWCC 1 cut(s) 630
BauI CACGAG 1 cut(s) 646
BbsI GAAGAC 1 cut(s) 334
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 2 cut(s) 117, 611
BccI CCATC 1 cut(s) 253
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 555
BcoDI GTCTC 5 cut(s) 459, 509, 515, 573, 692
BfuAI ACCTGC 2 cut(s) 308, 574
BisI GCNGC 2 cut(s) 131, 600
BlsI GCNGC 2 cut(s) 132, 601
Bme1390I CCNGG 1 cut(s) 555
Bme18I GGWCC 1 cut(s) 630
BmgT120I GGNCC 1 cut(s) 630
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 1 cut(s) 555
BpiI GAAGAC 1 cut(s) 334
Bpu10I CCTNAGC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 692
BsaJI CCNNGG 2 cut(s) 64, 143
BsaXI ACNNNNNCTCC 4 cut(s) 225, 255, 273, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 421
Bse1I ACTGG 3 cut(s) 220, 266, 657
Bse3DI GCAATG 1 cut(s) 594
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 64, 143
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 2 cut(s) 371, 421
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 4 cut(s) 70, 263, 324, 510
BseNI ACTGG 3 cut(s) 220, 266, 657
BseXI GCAGC 2 cut(s) 117, 611
BseYI CCCAGC 1 cut(s) 483
BsgI GTGCAG 1 cut(s) 286
Bsh1236I CGCG 1 cut(s) 689
BslFI GGGAC 1 cut(s) 643
BslI CCNNNNNNNGG 2 cut(s) 371, 421
BsmAI GTCTC 5 cut(s) 459, 509, 515, 573, 692
BsmBI CGTCTC 1 cut(s) 459
BsmFI GGGAC 1 cut(s) 643
BsmI GAATGC 2 cut(s) 444, 670
Bso31I GGTCTC 1 cut(s) 692
Bsp143I GATC 2 cut(s) 396, 489
BspCNI CTCAG 4 cut(s) 69, 262, 325, 511
BspFNI CGCG 1 cut(s) 689
BspLI GGNNCC 1 cut(s) 631
BspMI ACCTGC 2 cut(s) 308, 574
BspTNI GGTCTC 1 cut(s) 692
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 3 cut(s) 220, 266, 657
BssECI CCNNGG 2 cut(s) 64, 143
BssMI GATC 2 cut(s) 396, 489
BssSI CACGAG 1 cut(s) 646
Bst2BI CACGAG 1 cut(s) 646
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 5 cut(s) 103, 237, 476, 584, 634
Bst6I CTCTTC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 596
BstDEI CTNAG 4 cut(s) 56, 249, 333, 519
BstDSI CCRYGG 1 cut(s) 64
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 1 cut(s) 160
BstFNI CGCG 1 cut(s) 689
BstKTI GATC 2 cut(s) 399, 492
BstMAI GTCTC 5 cut(s) 459, 509, 515, 573, 692
BstMBI GATC 2 cut(s) 396, 489
BstMWI GCNNNNNNNGC 5 cut(s) 57, 66, 136, 329, 596
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 1 cut(s) 32
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 553
BstUI CGCG 1 cut(s) 689
BstV1I GCAGC 2 cut(s) 117, 611
BstV2I GAAGAC 1 cut(s) 334
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 1 cut(s) 160
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 396, 664
BveI ACCTGC 2 cut(s) 308, 574
Cfr13I GGNCC 1 cut(s) 630
Csp6I GTAC 1 cut(s) 233
CviAII CATG 3 cut(s) 29, 169, 446
CviJI RGCY 7 cut(s) 51, 60, 69, 109, 130, 483, 558
CviKI_1 RGCY 7 cut(s) 51, 60, 69, 109, 130, 483, 558
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 4 cut(s) 56, 249, 333, 519
DpnI GATC 2 cut(s) 398, 491
DpnII GATC 2 cut(s) 396, 489
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco31I GGTCTC 1 cut(s) 692
Eco47I GGWCC 1 cut(s) 630
Eco57I CTGAAG 1 cut(s) 291
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 553
Esp3I CGTCTC 1 cut(s) 459
FaeI CATG 3 cut(s) 32, 172, 449
FaiI YATR 9 cut(s) 18, 30, 170, 417, 447, 454, 498, 510, 705
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 1 cut(s) 643
FatI CATG 3 cut(s) 28, 168, 445
Fnu4HI GCNGC 2 cut(s) 131, 600
FokI GGATG 1 cut(s) 167
Fsp4HI GCNGC 2 cut(s) 131, 600
GluI GCNGC 2 cut(s) 131, 600
GsaI CCCAGC 1 cut(s) 487
Hin1II CATG 3 cut(s) 32, 172, 449
HinfI GANTC 3 cut(s) 54, 209, 407
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 2 cut(s) 34, 208
Hpy188I TCNGA 5 cut(s) 144, 252, 281, 350, 381
Hpy188III TCNNGA 2 cut(s) 309, 529
Hpy8I GTNNAC 2 cut(s) 34, 208
HpyAV CCTTC 2 cut(s) 142, 428
HpyCH4III ACNGT 5 cut(s) 103, 237, 476, 584, 634
HpyCH4V TGCA 3 cut(s) 133, 303, 599
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 66, 136, 329, 596
HpyF3I CTNAG 4 cut(s) 56, 249, 333, 519
Hsp92II CATG 3 cut(s) 32, 172, 449
Kzo9I GATC 2 cut(s) 396, 489
LmnI GCTCC 2 cut(s) 65, 447
Lsp1109I GCAGC 2 cut(s) 117, 611
MaeIII GTNAC 2 cut(s) 237, 295
MalI GATC 2 cut(s) 398, 491
MboI GATC 2 cut(s) 396, 489
MboII GAAGA 5 cut(s) 3, 87, 106, 284, 334
MfeI CAATTG 1 cut(s) 134
MluCI AATT 5 cut(s) 134, 188, 315, 603, 613
MlyI GAGTC 2 cut(s) 48, 203
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 6 cut(s) 97, 138, 286, 328, 368, 421
MseI TTAA 2 cut(s) 81, 161
MspA1I CMGCKG 1 cut(s) 130
MspR9I CCNGG 1 cut(s) 555
MunI CAATTG 1 cut(s) 134
Mva1269I GAATGC 2 cut(s) 444, 670
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 1 cut(s) 689
MwoI GCNNNNNNNGC 5 cut(s) 57, 66, 136, 329, 596
NdeII GATC 2 cut(s) 396, 489
NlaIII CATG 3 cut(s) 32, 172, 449
NlaIV GGNNCC 1 cut(s) 631
NmuCI GTSAC 1 cut(s) 237
NspI RCATGY 1 cut(s) 32
PciI ACATGT 1 cut(s) 28
PctI GAATGC 2 cut(s) 444, 670
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 2 cut(s) 132, 601
PleI GAGTC 2 cut(s) 48, 203
PpsI GAGTC 2 cut(s) 48, 203
PscI ACATGT 1 cut(s) 28
PsiI TTATAA 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 553
PspEI GGTNACC 1 cut(s) 295
PspFI CCCAGC 1 cut(s) 483
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 1 cut(s) 631
PspPI GGNCC 1 cut(s) 630
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SaqAI TTAA 2 cut(s) 81, 161
SatI GCNGC 2 cut(s) 131, 600
Sau3AI GATC 2 cut(s) 396, 489
Sau96I GGNCC 1 cut(s) 630
SchI GAGTC 2 cut(s) 48, 203
ScrFI CCNGG 1 cut(s) 555
SinI GGWCC 1 cut(s) 630
Sse9I AATT 5 cut(s) 134, 188, 315, 603, 613
StyD4I CCNGG 1 cut(s) 553
TaaI ACNGT 5 cut(s) 103, 237, 476, 584, 634
TaqI TCGA 1 cut(s) 395
TasI AATT 5 cut(s) 134, 188, 315, 603, 613
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 2 cut(s) 81, 161
Tru9I TTAA 2 cut(s) 81, 161
TscAI CASTG 2 cut(s) 396, 664
TseFI GTSAC 1 cut(s) 237
TseI GCWGC 2 cut(s) 130, 599
Tsp45I GTSAC 1 cut(s) 237
TspDTI ATGAA 2 cut(s) 185, 334
TspRI CASTG 2 cut(s) 396, 664
VpaK11BI GGWCC 1 cut(s) 630
XapI RAATTY 2 cut(s) 315, 613
XceI RCATGY 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.