Rh6DG460000

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002862
Physical Location & Seq
Reverse (-)
62954060 .. 62958673
4614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG460000.1

Sequence Viewer

Length: 1701 bp
ATGGCAATCTCTTCATATAATGCTTTACAAGTAAACGGGTCATTGCAAAGCCGTCTCATCTCCCACAGCTTTAATCATCTCAAAACCTCACTTTCCTCAACTGTTAAGCCTTTCTTCAAAGAGTTGCAGCAATTGCTTCCGATGAAGGTGGATGTCTCGAAAACCATGAAGAACACTTCCGGGAAAATGTTAGATGCAGTTGTGGACTCCCTGTTCCAATTTGTGGATATACCCTCACTCCCATCTCAGAAAAACTTTTTACCCGTTGAAGAGATCGGAGAACTTGTTGAGGTCACCTGCACCTTAGGAGAAATTCCTGCTGATTTTGCGGAGGGTGTTTTCATCAGAAATGGCAGTAATCCTTTGTTTGGAGGTCTAAAATCAGCAGTTTCGATCTTCGGAGAATCACACAATATTTGGGTAGAGGGAGAAGGAATGCTCCATGCTCTATACTTCAAGAAAGACGATGATTGTGGAAGTTGGATAATCTCATATAAGAATAGATATGTTGAGAGTGAGACATTTAAGATAGAAACTCAACGAAATAAACCAGGTTTCTTACCGGCCCTTGCAGGAGACGCTCCAGCAATTATGGCAGCATTTCTTTTAAATTCGTTGAGATTTGGGACAGTCATTAAACACATGAGCAACACCAATGTTTTCGAGCATTCCGGGAGAATTTACTCAATTGCAGAGAACTATCTGCCTCAAGAGGTGGACATTTCAACCTTGGAAACCATTTGTGATTGGGATGTCAATGGAACTTGGGATCGACCTTTCACAAGTCATCCAAAGAAAGCTCCAAACGGAGAGTTAGTCGTGATGGGGACAGATGCATGGAAACCTTACTATGTTTTAGGGGTCATCTCTGCTGATGGAAAGAAAGTGCACAAGGTTGATCTCAAATTTAAAAGATGTGTGTTCAGTCATGATATAGGAGTCACGCAAAACTACAATGTAATCCTTGATCATCCCCTCACATTGGATGTAAAGAGACTCACCATGGGAGGCCAAATAATGCAGTATGAAAAGGAAGAGTACGCTAGAATTGGGGTCATGCCTCGTTATGGAGATGCAGAGTCTGTCAAATGGTTTGAGGTACAGACCAATTGCACACTTCACTTTCTCAATTGCTTTGAGGATGCTAATCAGGTTATAGTCAGGGGATGTAGGGCTCTCACGTCTGTTGTATCAGATCCTGACGGTAGCCTAAACCCTTATGAATGGTTCTCCAAAGGGTTCATCTTTGCTGGTGATCATGATTCTGCAGAGGTTGGATATCTCTTTACGCATTTATACGAATGGAGATTGAACATGTTATCTGGGAAAGTTGAGGAGAGAAACTTAACTGGATCTGAATACTCCATGGATTTCCCCTTCATCAATGAACAATTTACTGGTTTAAAACACAGTTATGGATATACTCAGGTCATTGATTCAGGGGCAAGCTCTATATGTGGCATGGCTAAATTTGGATCTCTAGCCAAATTATATTTGGAAGAATCAGATTCTACAAAATCTGTGGAGGGAAGGGGTGAGCACTTGATAAAGGTAGAATACCATAAGTTTGAGGACAATAACTTTTGCAGTGGAAGTGCTTTTGTCCCCAGAAATGGAGGCGAGGAAGATGATGGCTGGATTATCACTTTTGTCCACAATGAAGAAACTGATGTGACCCAAAGACAACTTTTTAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

566

Amino Acids

63.65

Weight (kDa)

5.34

Isoelectric Point (pI)

26.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 89 - 557 6.8e-94 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 305
AccB7I CCANNNNNTGG 1 cut(s) 223
AciI CCGC 1 cut(s) 329
AclWI GGATC 4 cut(s) 777, 1190, 1360, 1483
AcsI RAATTY 5 cut(s) 312, 610, 678, 905, 1469
AfaI GTAC 2 cut(s) 1040, 1101
AfiI CCNNNNNNNGG 5 cut(s) 223, 368, 982, 1067, 1613
AflIII ACRYGT 1 cut(s) 1314
AgsI TTSAA 5 cut(s) 118, 269, 457, 726, 1312
AjiI CACGTC 1 cut(s) 1182
AjnI CCWGG 1 cut(s) 550
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 3 cut(s) 69, 800, 1449
AluI AGCT 3 cut(s) 69, 800, 1449
Alw21I GWGCWC 2 cut(s) 891, 1542
Alw26I GTCTC 5 cut(s) 59, 160, 512, 570, 988
Alw44I GTGCAC 1 cut(s) 887
AlwI GGATC 4 cut(s) 777, 1190, 1360, 1483
AlwNI CAGNNNCTG 2 cut(s) 1082, 1199
AoxI GGCC 2 cut(s) 564, 1009
ApaLI GTGCAC 1 cut(s) 887
ApeKI GCWGC 2 cut(s) 127, 596
ApoI RAATTY 5 cut(s) 312, 610, 678, 905, 1469
AspS9I GGNCC 1 cut(s) 565
AsuC2I CCSGG 2 cut(s) 181, 673
AsuHPI GGTGA 4 cut(s) 286, 991, 1265, 1547
AxyI CCTNAGG 1 cut(s) 304
BaeGI GKGCMC 1 cut(s) 891
BanII GRGCYC 1 cut(s) 1177
Bbv12I GWGCWC 2 cut(s) 891, 1542
BbvI GCAGC 2 cut(s) 139, 608
BccI CCATC 4 cut(s) 250, 817, 869, 1625
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 552
BclI TGATCA 2 cut(s) 967, 1255
BcnI CCSGG 2 cut(s) 181, 673
BcoDI GTCTC 5 cut(s) 59, 160, 512, 570, 988
BfaI CTAG 2 cut(s) 1044, 1481
BfmI CTRYAG 1 cut(s) 1266
BfuAI ACCTGC 1 cut(s) 305
BisI GCNGC 2 cut(s) 128, 597
BlsI GCNGC 2 cut(s) 129, 598
Bme1390I CCNGG 3 cut(s) 181, 552, 673
BmgBI CACGTC 1 cut(s) 1182
BmgT120I GGNCC 1 cut(s) 565
BmrFI CCNGG 3 cut(s) 181, 552, 673
BmsI GCATC 4 cut(s) 184, 823, 1063, 1132
BpmI CTGGAG 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 693
BpuMI CCSGG 2 cut(s) 181, 673
BsaBI GATNNNNATC 1 cut(s) 1146
BsaJI CCNNGG 3 cut(s) 729, 1002, 1365
BsaXI ACNNNNNCTCC 6 cut(s) 222, 252, 270, 300, 801, 831
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 368, 982, 1067, 1613
Bse118I RCCGGY 1 cut(s) 562
Bse1I ACTGG 2 cut(s) 1354, 1402
Bse21I CCTNAGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 1146
BseBI CCWGG 1 cut(s) 552
BseDI CCNNGG 3 cut(s) 729, 1002, 1365
BseGI GGATG 7 cut(s) 157, 757, 787, 970, 991, 1147, 1172
BseJI GATNNNNATC 1 cut(s) 1146
BseLI CCNNNNNNNGG 5 cut(s) 223, 368, 982, 1067, 1613
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 2 cut(s) 260, 1439
BseNI ACTGG 2 cut(s) 1354, 1402
BseRI GAGGAG 1 cut(s) 1349
BseSI GKGCMC 1 cut(s) 891
BseXI GCAGC 2 cut(s) 139, 608
BsgI GTGCAG 1 cut(s) 283
BshFI GGCC 2 cut(s) 566, 1011
BsiHKAI GWGCWC 2 cut(s) 891, 1542
BsiSI CCGG 3 cut(s) 180, 563, 672
BslFI GGGAC 3 cut(s) 640, 841, 1589
BslI CCNNNNNNNGG 5 cut(s) 223, 368, 982, 1067, 1613
BsmAI GTCTC 5 cut(s) 59, 160, 512, 570, 988
BsmBI CGTCTC 2 cut(s) 59, 570
BsmFI GGGAC 3 cut(s) 640, 841, 1589
BsmI GAATGC 2 cut(s) 441, 667
BsnI GGCC 2 cut(s) 566, 1011
Bsp1286I GDGCHC 3 cut(s) 891, 1177, 1542
Bsp143I GATC 9 cut(s) 273, 393, 769, 898, 967, 1195, 1255, 1352, 1475
Bsp19I CCATGG 2 cut(s) 1002, 1365
BspACI CCGC 1 cut(s) 329
BspANI GGCC 2 cut(s) 566, 1011
BspCNI CTCAG 2 cut(s) 259, 1438
BspHI TCATGA 2 cut(s) 928, 1258
BspMAI CTGCAG 1 cut(s) 1270
BspMI ACCTGC 1 cut(s) 305
BspPI GGATC 4 cut(s) 777, 1190, 1360, 1483
BsrDI GCAATG 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 562
BsrI ACTGG 2 cut(s) 1354, 1402
BssAI RCCGGY 1 cut(s) 562
BssECI CCNNGG 3 cut(s) 729, 1002, 1365
BssMI GATC 9 cut(s) 273, 393, 769, 898, 967, 1195, 1255, 1352, 1475
BssT1I CCWWGG 3 cut(s) 729, 1002, 1365
Bst2UI CCWGG 1 cut(s) 552
Bst4CI ACNGT 4 cut(s) 103, 631, 1205, 1412
Bst6I CTCTTC 3 cut(s) 16, 264, 1029
BstAPI GCANNNNNTGC 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 1447
BstDEI CTNAG 3 cut(s) 246, 304, 1425
BstDSI CCRYGG 2 cut(s) 1002, 1365
BstEII GGTNACC 1 cut(s) 292
BstF5I GGATG 7 cut(s) 157, 757, 787, 970, 991, 1147, 1172
BstKTI GATC 9 cut(s) 276, 396, 772, 901, 970, 1198, 1258, 1355, 1478
BstMAI GTCTC 5 cut(s) 59, 160, 512, 570, 988
BstMBI GATC 9 cut(s) 273, 393, 769, 898, 967, 1195, 1255, 1352, 1475
BstMWI GCNNNNNNNGC 4 cut(s) 133, 326, 578, 593
BstNI CCWGG 1 cut(s) 552
BstNSI RCATGY 1 cut(s) 1318
BstPI GGTNACC 1 cut(s) 292
BstSCI CCNGG 3 cut(s) 179, 550, 671
BstSFI CTRYAG 1 cut(s) 1266
BstSLI GKGCMC 1 cut(s) 891
BstV1I GCAGC 2 cut(s) 139, 608
BstX2I RGATCY 3 cut(s) 1195, 1352, 1475
BstYI RGATCY 3 cut(s) 1195, 1352, 1475
Bsu36I CCTNAGG 1 cut(s) 304
BsuRI GGCC 2 cut(s) 566, 1011
BtgI CCRYGG 2 cut(s) 1002, 1365
BtrI CACGTC 1 cut(s) 1182
BtsCI GGATG 7 cut(s) 157, 757, 787, 970, 991, 1147, 1172
BtsI GCAGTG 1 cut(s) 1594
BtsIMutI CAGTG 1 cut(s) 1594
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 1 cut(s) 1447
CaiI CAGNNNCTG 2 cut(s) 1082, 1199
CciI TCATGA 2 cut(s) 928, 1258
Cfr10I RCCGGY 1 cut(s) 562
Cfr13I GGNCC 1 cut(s) 565
CseI GACGC 1 cut(s) 587
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 2 cut(s) 1039, 1100
CspCI CAANNNNNGTGG 2 cut(s) 1503, 1538
CviQI GTAC 2 cut(s) 1039, 1100
DdeI CTNAG 3 cut(s) 246, 304, 1425
DpnI GATC 9 cut(s) 275, 395, 771, 900, 969, 1197, 1257, 1354, 1477
DpnII GATC 9 cut(s) 273, 393, 769, 898, 967, 1195, 1255, 1352, 1475
DraI TTTAAA 3 cut(s) 609, 910, 1404
Eam1104I CTCTTC 3 cut(s) 16, 264, 1029
EarI CTCTTC 3 cut(s) 16, 264, 1029
Eco130I CCWWGG 3 cut(s) 729, 1002, 1365
Eco24I GRGCYC 1 cut(s) 1177
Eco32I GATATC 1 cut(s) 1280
Eco81I CCTNAGG 1 cut(s) 304
Eco91I GGTNACC 1 cut(s) 292
EcoO65I GGTNACC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 550
EcoRV GATATC 1 cut(s) 1280
EcoT14I CCWWGG 3 cut(s) 729, 1002, 1365
EcoT22I ATGCAT 1 cut(s) 838
EcoT38I GRGCYC 1 cut(s) 1177
ErhI CCWWGG 3 cut(s) 729, 1002, 1365
Esp3I CGTCTC 2 cut(s) 59, 570
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 3 cut(s) 640, 841, 1589
FbaI TGATCA 2 cut(s) 967, 1255
Fnu4HI GCNGC 2 cut(s) 128, 597
FokI GGATG 7 cut(s) 164, 764, 774, 957, 998, 1154, 1179
FriOI GRGCYC 1 cut(s) 1177
Fsp4HI GCNGC 2 cut(s) 128, 597
FspBI CTAG 2 cut(s) 1044, 1481
GluI GCNGC 2 cut(s) 128, 597
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 2 cut(s) 566, 1011
HapII CCGG 3 cut(s) 180, 563, 672
HgaI GACGC 1 cut(s) 587
HinfI GANTC 9 cut(s) 206, 404, 939, 996, 1079, 1262, 1436, 1502, 1508
HpaII CCGG 3 cut(s) 180, 563, 672
HphI GGTGA 4 cut(s) 286, 991, 1265, 1547
Hpy166II GTNNAC 5 cut(s) 34, 205, 718, 889, 1654
Hpy188I TCNGA 8 cut(s) 141, 249, 278, 347, 401, 1195, 1357, 1507
Hpy188III TCNNGA 7 cut(s) 157, 457, 710, 820, 929, 1199, 1259
Hpy8I GTNNAC 5 cut(s) 34, 205, 718, 889, 1654
HpyAV CCTTC 4 cut(s) 139, 425, 1387, 1524
HpyCH4III ACNGT 4 cut(s) 103, 631, 1205, 1412
HpyCH4IV ACGT 1 cut(s) 1181
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 326, 578, 593
HpyF3I CTNAG 3 cut(s) 246, 304, 1425
HpySE526I ACGT 1 cut(s) 1181
Ksp22I TGATCA 2 cut(s) 967, 1255
Kzo9I GATC 9 cut(s) 273, 393, 769, 898, 967, 1195, 1255, 1352, 1475
LmnI GCTCC 3 cut(s) 444, 586, 805
Lsp1109I GCAGC 2 cut(s) 139, 608
LweI GCATC 4 cut(s) 184, 823, 1063, 1132
MabI ACCWGGT 1 cut(s) 550
MaeI CTAG 2 cut(s) 1044, 1481
MaeII ACGT 1 cut(s) 1181
MaeIII GTNAC 3 cut(s) 292, 940, 1672
MalI GATC 9 cut(s) 275, 395, 771, 900, 969, 1197, 1257, 1354, 1477
MboI GATC 9 cut(s) 273, 393, 769, 898, 967, 1195, 1255, 1352, 1475
MboII GAAGA 9 cut(s) 3, 106, 181, 281, 388, 1046, 1511, 1637, 1673
MfeI CAATTG 4 cut(s) 131, 687, 1108, 1129
MflI RGATCY 3 cut(s) 1195, 1352, 1475
MhlI GDGCHC 3 cut(s) 891, 1177, 1542
MlyI GAGTC 4 cut(s) 200, 948, 990, 1088
MmeI TCCRAC 2 cut(s) 461, 1255
Mph1103I ATGCAT 1 cut(s) 838
MseI TTAA 8 cut(s) 72, 105, 525, 608, 636, 909, 1346, 1403
MslI CAYNNNNRTG 1 cut(s) 1413
MspI CCGG 3 cut(s) 180, 563, 672
MspR9I CCNGG 3 cut(s) 181, 552, 673
MunI CAATTG 4 cut(s) 131, 687, 1108, 1129
Mva1269I GAATGC 2 cut(s) 441, 667
MvaI CCWGG 1 cut(s) 552
MwoI GCNNNNNNNGC 4 cut(s) 133, 326, 578, 593
NciI CCSGG 2 cut(s) 181, 673
NcoI CCATGG 2 cut(s) 1002, 1365
NdeII GATC 9 cut(s) 273, 393, 769, 898, 967, 1195, 1255, 1352, 1475
NmuCI GTSAC 3 cut(s) 292, 940, 1672
NsiI ATGCAT 1 cut(s) 838
NspI RCATGY 1 cut(s) 1318
PagI TCATGA 2 cut(s) 928, 1258
PaqCI CACCTGC 1 cut(s) 305
PciI ACATGT 1 cut(s) 1314
PctI GAATGC 2 cut(s) 441, 667
PfeI GAWTC 5 cut(s) 404, 1262, 1436, 1502, 1508
PflMI CCANNNNNTGG 1 cut(s) 223
PfoI TCCNGGA 2 cut(s) 179, 671
PkrI GCNGC 2 cut(s) 129, 598
PleI GAGTC 4 cut(s) 200, 947, 990, 1087
PpsI GAGTC 4 cut(s) 200, 947, 990, 1087
PscI ACATGT 1 cut(s) 1314
Psp6I CCWGG 1 cut(s) 550
PspEI GGTNACC 1 cut(s) 292
PspGI CCWGG 1 cut(s) 550
PspPI GGNCC 1 cut(s) 565
PstI CTGCAG 1 cut(s) 1270
PstNI CAGNNNCTG 2 cut(s) 1082, 1199
PsuI RGATCY 3 cut(s) 1195, 1352, 1475
RsaI GTAC 2 cut(s) 1040, 1101
RsaNI GTAC 2 cut(s) 1039, 1100
RseI CAYNNNNRTG 1 cut(s) 1413
SaqAI TTAA 8 cut(s) 72, 105, 525, 608, 636, 909, 1346, 1403
SatI GCNGC 2 cut(s) 128, 597
Sau3AI GATC 9 cut(s) 273, 393, 769, 898, 967, 1195, 1255, 1352, 1475
Sau96I GGNCC 1 cut(s) 565
SchI GAGTC 4 cut(s) 200, 948, 990, 1088
ScrFI CCNGG 3 cut(s) 181, 552, 673
SduI GDGCHC 3 cut(s) 891, 1177, 1542
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 4 cut(s) 184, 823, 1063, 1132
SfcI CTRYAG 1 cut(s) 1266
SmiMI CAYNNNNRTG 1 cut(s) 1413
SmlI CTYRAG 1 cut(s) 708
SmoI CTYRAG 1 cut(s) 708
SsiI CCGC 1 cut(s) 329
SspI AATATT 1 cut(s) 415
SspMI CTAG 2 cut(s) 1044, 1481
StyD4I CCNGG 3 cut(s) 179, 550, 671
StyI CCWWGG 3 cut(s) 729, 1002, 1365
TaaI ACNGT 4 cut(s) 103, 631, 1205, 1412
TaiI ACGT 1 cut(s) 1184
TaqI TCGA 4 cut(s) 158, 392, 663, 772
TfiI GAWTC 5 cut(s) 404, 1262, 1436, 1502, 1508
Tru1I TTAA 8 cut(s) 72, 105, 525, 608, 636, 909, 1346, 1403
Tru9I TTAA 8 cut(s) 72, 105, 525, 608, 636, 909, 1346, 1403
TscAI CASTG 1 cut(s) 1594
TseFI GTSAC 3 cut(s) 292, 940, 1672
TseI GCWGC 2 cut(s) 127, 596
Tsp45I GTSAC 3 cut(s) 292, 940, 1672
TspDTI ATGAA 9 cut(s) 158, 182, 331, 1041, 1231, 1236, 1369, 1401, 1674
TspGWI ACGGA 1 cut(s) 822
TspRI CASTG 1 cut(s) 1594
Van91I CCANNNNNTGG 1 cut(s) 223
VneI GTGCAC 1 cut(s) 887
XapI RAATTY 5 cut(s) 312, 610, 678, 905, 1469
XceI RCATGY 1 cut(s) 1318
XcmI CCANNNNNNNNNTGG 1 cut(s) 1492
XspI CTAG 2 cut(s) 1044, 1481
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.