RchiOBHm_Chr6g0306121

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002862
Physical Location & Seq
Reverse (-)
64867852 .. 64871627
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27514

Sequence Viewer

Length: 993 bp
ATGATCGTGTGCTTGCAGAAAAACTTTTTACCTATTGAAGAGATCGGAGAACTTGTTGAAGTCACCTGCACCTTAGGAGAAATTCCTGCTGATTTTGCTGAGGGCAGTAATCCTTTGTTTGGAGGTCTAAAATCAGCAGTTTCGATCTTCGGAGAATCACACAATATTTGGGTAGAGGGAGAAGGAATGCTCCATGCTCTATACTTCAAGAAAGACGATGACTGTGGAAGTTGGATCATCTCATATAAGAATAGATATGTTGAGAGTGAGACATTTAAGATAGAAACTCAACGAAATAAACCAGGTTTCTTACCGGCCCTTGCAGGAGACGCTCCAGCAATTATAGCAGCATTTCTTTTAAATTCGTTGAGATTTGGGACAGTCATCAAACACATGAGCAACACCAATGTTTTCGAGCATTCAGGGAAAATTTACTCAATTGCAGAGAACTATCTACCTCAAGAGGTGGACATTTCAACCTTGGAAACCATTTGTGATTGGGATGTCAATGGAACTTGGGATCGACCTTTCACAAGTCATCCAAAGTATGAAAAGGAAGAGTACGCTAGAATTGGGGTCATGCCTCGTTATGGAGATGCAGAGTCTGTCAAATGGTTTGAGGTTGGATATCTCTTTACGCATTTATACGAATGGAGATTGAACATGTTATCTGGGAAAGTTGAGGAGAGAAACTTAACTGGATCCGAATACTCCATGGATTTCCCTTTCATCAATGAACAATTTACTGGTTTAAAACACAAGTATGGATATACTCAGGTCATTGATTCCGGGGCAAGCTCTATATGTGGCATCGAGGAAGATGATGGCTGGATTATCACTTTTGTCCACAATGAAGAAACTGATGTGACCCAAGTTCATATCATTGATGCACAGGATTTTGAAGGTGAAGCGATTGCGAAACTTACATTGCCACAACGGGTGCCCTATGGTTTTCATTCAACCTTTCTGTCAATGCCAGCTTGTCAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

330

Amino Acids

37.34

Weight (kDa)

4.71

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 14 - 186 2.6e-26 Retinal pigment epithelial membrane protein
RPE65 PF03055 213 - 264 5.8e-07 Retinal pigment epithelial membrane protein
RPE65 PF03055 272 - 323 4.7e-16 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 940
AclWI GGATC 4 cut(s) 242, 528, 696, 709
AcsI RAATTY 3 cut(s) 81, 361, 429
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 2 cut(s) 119, 590
AflIII ACRYGT 1 cut(s) 663
AgsI TTSAA 7 cut(s) 38, 59, 208, 477, 661, 902, 960
AjnI CCWGG 1 cut(s) 301
AluBI AGCT 2 cut(s) 798, 980
AluI AGCT 2 cut(s) 798, 980
Alw26I GTCTC 2 cut(s) 263, 321
AlwI GGATC 4 cut(s) 242, 528, 696, 709
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 347
ApoI RAATTY 3 cut(s) 81, 361, 429
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 790
AsuHPI GGTGA 2 cut(s) 55, 917
AxyI CCTNAGG 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 945
BamHI GGATCC 1 cut(s) 701
BanI GGYRCC 1 cut(s) 940
BbvCI CCTCAGC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 359
BccI CCATC 1 cut(s) 818
BciT130I CCWGG 1 cut(s) 303
BcnI CCSGG 1 cut(s) 790
BcoDI GTCTC 2 cut(s) 263, 321
BfaI CTAG 1 cut(s) 567
BfuAI ACCTGC 1 cut(s) 74
BisI GCNGC 1 cut(s) 348
BlsI GCNGC 1 cut(s) 349
Bme1390I CCNGG 2 cut(s) 303, 790
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 2 cut(s) 703, 942
BmrFI CCNGG 2 cut(s) 303, 790
BmsI GCATC 3 cut(s) 586, 819, 877
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 790
BsaJI CCNNGG 3 cut(s) 480, 714, 789
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 590
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 2 cut(s) 703, 751
Bse21I CCTNAGG 1 cut(s) 73
Bse3DI GCAATG 1 cut(s) 926
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 3 cut(s) 480, 714, 789
BseGI GGATG 2 cut(s) 508, 538
BseLI CCNNNNNNNGG 2 cut(s) 119, 590
BseMI GCAATG 1 cut(s) 926
BseMII CTCAG 2 cut(s) 90, 788
BseNI ACTGG 2 cut(s) 703, 751
BseRI GAGGAG 1 cut(s) 698
BseSI GKGCMC 1 cut(s) 945
BseXI GCAGC 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 52
BshFI GGCC 1 cut(s) 317
BshNI GGYRCC 1 cut(s) 940
BsiSI CCGG 2 cut(s) 314, 789
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 2 cut(s) 119, 590
BsmAI GTCTC 2 cut(s) 263, 321
BsmBI CGTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 391
BsmI GAATGC 2 cut(s) 192, 418
BsnI GGCC 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 945
Bsp143I GATC 6 cut(s) 3, 42, 144, 234, 520, 701
Bsp19I CCATGG 1 cut(s) 714
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 2 cut(s) 91, 787
BspLI GGNNCC 2 cut(s) 703, 942
BspMI ACCTGC 1 cut(s) 74
BspPI GGATC 4 cut(s) 242, 528, 696, 709
BspT107I GGYRCC 1 cut(s) 940
BsrDI GCAATG 1 cut(s) 926
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 2 cut(s) 703, 751
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 3 cut(s) 480, 714, 789
BssMI GATC 6 cut(s) 3, 42, 144, 234, 520, 701
BssT1I CCWWGG 2 cut(s) 480, 714
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 224, 382
Bst6I CTCTTC 2 cut(s) 33, 552
BstC8I GCNNGC 3 cut(s) 14, 796, 978
BstDEI CTNAG 4 cut(s) 73, 99, 774, 990
BstDSI CCRYGG 1 cut(s) 714
BstF5I GGATG 2 cut(s) 508, 538
BstKTI GATC 6 cut(s) 6, 45, 147, 237, 523, 704
BstMAI GTCTC 2 cut(s) 263, 321
BstMBI GATC 6 cut(s) 3, 42, 144, 234, 520, 701
BstMWI GCNNNNNNNGC 3 cut(s) 95, 329, 344
BstNI CCWGG 1 cut(s) 303
BstNSI RCATGY 1 cut(s) 667
BstSCI CCNGG 2 cut(s) 301, 788
BstSLI GKGCMC 1 cut(s) 945
BstV1I GCAGC 1 cut(s) 359
BstX2I RGATCY 1 cut(s) 701
BstYI RGATCY 1 cut(s) 701
Bsu36I CCTNAGG 1 cut(s) 73
BsuRI GGCC 1 cut(s) 317
BtgI CCRYGG 1 cut(s) 714
BtsCI GGATG 2 cut(s) 508, 538
BveI ACCTGC 1 cut(s) 74
Cac8I GCNNGC 3 cut(s) 14, 796, 978
CaiI CAGNNNCTG 1 cut(s) 605
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 1 cut(s) 338
CsiI ACCWGGT 1 cut(s) 301
Csp6I GTAC 1 cut(s) 562
CviAII CATG 5 cut(s) 194, 394, 580, 664, 715
CviJI RGCY 4 cut(s) 317, 798, 828, 980
CviKI_1 RGCY 4 cut(s) 317, 798, 828, 980
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 4 cut(s) 73, 99, 774, 990
DpnI GATC 6 cut(s) 5, 44, 146, 236, 522, 703
DpnII GATC 6 cut(s) 3, 42, 144, 234, 520, 701
DraI TTTAAA 2 cut(s) 360, 753
Eam1104I CTCTTC 2 cut(s) 33, 552
EarI CTCTTC 2 cut(s) 33, 552
Eco130I CCWWGG 2 cut(s) 480, 714
Eco32I GATATC 1 cut(s) 629
Eco81I CCTNAGG 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 301
EcoRV GATATC 1 cut(s) 629
EcoT14I CCWWGG 2 cut(s) 480, 714
ErhI CCWWGG 2 cut(s) 480, 714
Esp3I CGTCTC 1 cut(s) 321
FaeI CATG 5 cut(s) 197, 397, 583, 667, 718
FaqI GGGAC 1 cut(s) 391
FatI CATG 5 cut(s) 193, 393, 579, 663, 714
Fnu4HI GCNGC 1 cut(s) 348
FokI GGATG 2 cut(s) 515, 525
Fsp4HI GCNGC 1 cut(s) 348
FspBI CTAG 1 cut(s) 567
GluI GCNGC 1 cut(s) 348
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 1 cut(s) 317
HapII CCGG 2 cut(s) 314, 789
HgaI GACGC 1 cut(s) 338
Hin1II CATG 5 cut(s) 197, 397, 583, 667, 718
HinfI GANTC 3 cut(s) 155, 602, 785
HpaII CCGG 2 cut(s) 314, 789
HphI GGTGA 2 cut(s) 55, 917
Hpy166II GTNNAC 2 cut(s) 469, 847
Hpy188I TCNGA 3 cut(s) 47, 152, 706
Hpy188III TCNNGA 2 cut(s) 208, 461
Hpy8I GTNNAC 2 cut(s) 469, 847
HpyAV CCTTC 2 cut(s) 176, 896
HpyCH4III ACNGT 2 cut(s) 224, 382
HpyCH4V TGCA 6 cut(s) 16, 69, 323, 443, 599, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 329, 344
HpyF3I CTNAG 4 cut(s) 73, 99, 774, 990
Hsp92II CATG 5 cut(s) 197, 397, 583, 667, 718
Kzo9I GATC 6 cut(s) 3, 42, 144, 234, 520, 701
LmnI GCTCC 2 cut(s) 195, 337
Lsp1109I GCAGC 1 cut(s) 359
LweI GCATC 3 cut(s) 586, 819, 877
MabI ACCWGGT 1 cut(s) 301
MaeI CTAG 1 cut(s) 567
MaeIII GTNAC 2 cut(s) 61, 865
MalI GATC 6 cut(s) 5, 44, 146, 236, 522, 703
MboI GATC 6 cut(s) 3, 42, 144, 234, 520, 701
MboII GAAGA 5 cut(s) 50, 139, 569, 830, 866
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 1 cut(s) 701
MhlI GDGCHC 1 cut(s) 945
MluCI AATT 7 cut(s) 81, 339, 361, 429, 438, 570, 740
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 2 cut(s) 212, 604
MnlI CCTC 9 cut(s) 94, 116, 169, 457, 468, 594, 613, 676, 808
MseI TTAA 4 cut(s) 276, 359, 695, 752
MslI CAYNNNNRTG 1 cut(s) 762
MspI CCGG 2 cut(s) 314, 789
MspR9I CCNGG 2 cut(s) 303, 790
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 2 cut(s) 192, 418
MvaI CCWGG 1 cut(s) 303
MwoI GCNNNNNNNGC 3 cut(s) 95, 329, 344
NciI CCSGG 1 cut(s) 790
NcoI CCATGG 1 cut(s) 714
NdeII GATC 6 cut(s) 3, 42, 144, 234, 520, 701
NlaIII CATG 5 cut(s) 197, 397, 583, 667, 718
NlaIV GGNNCC 2 cut(s) 703, 942
NmuCI GTSAC 2 cut(s) 61, 865
NspI RCATGY 1 cut(s) 667
PaqCI CACCTGC 1 cut(s) 74
PciI ACATGT 1 cut(s) 663
PctI GAATGC 2 cut(s) 192, 418
PfeI GAWTC 2 cut(s) 155, 785
PkrI GCNGC 1 cut(s) 349
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
PscI ACATGT 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 301
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 2 cut(s) 703, 942
PspPI GGNCC 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 1 cut(s) 701
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 1 cut(s) 762
SaqAI TTAA 4 cut(s) 276, 359, 695, 752
SatI GCNGC 1 cut(s) 348
Sau3AI GATC 6 cut(s) 3, 42, 144, 234, 520, 701
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 2 cut(s) 303, 790
SduI GDGCHC 1 cut(s) 945
SexAI ACCWGGT 1 cut(s) 301
SfaNI GCATC 3 cut(s) 586, 819, 877
SmiMI CAYNNNNRTG 1 cut(s) 762
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 7 cut(s) 81, 339, 361, 429, 438, 570, 740
SspI AATATT 1 cut(s) 166
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 301, 788
StyI CCWWGG 2 cut(s) 480, 714
TaaI ACNGT 2 cut(s) 224, 382
TaqI TCGA 4 cut(s) 143, 414, 523, 813
TasI AATT 7 cut(s) 81, 339, 361, 429, 438, 570, 740
TfiI GAWTC 2 cut(s) 155, 785
Tru1I TTAA 4 cut(s) 276, 359, 695, 752
Tru9I TTAA 4 cut(s) 276, 359, 695, 752
TseFI GTSAC 2 cut(s) 61, 865
TseI GCWGC 1 cut(s) 347
Tsp45I GTSAC 2 cut(s) 61, 865
TspDTI ATGAA 6 cut(s) 564, 718, 750, 866, 867, 944
XapI RAATTY 3 cut(s) 81, 361, 429
XceI RCATGY 1 cut(s) 667
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.