Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0016674

Physical Location & Seq

Genomic Coordinates Reverse (-)

6621128 .. 6622659

Gene Structure 1532 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1094400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 747 bp

ATGGCGAAAATAGCCTTGGGAACCACCGGTGAGGGTGCCCTTCCAGAAGTCTGGAGAGCTCTTGTGGTTGAGTTTATCACTACCTTCCTCTTCGTCTTTGCTGGTGTTGCATCAGCCATGGCTACTGATAAGCTAGCAGCAGATGCACTAGTGGGGTTGTTTGCCGTAGCTGTGACACATGCACTAGTTGTGGCCGTGATGATATCCGCCGGCCACATATCTGGCGGTCACCTTAACCCTGCCGTCACTCTTGGCCTCCTGTTTGGTGGTCATATTACCCTCTTCCGATCTATACTCTACTGGATCGATCAATTGTTAGCAGCTGCTGCAGCTTGTTACCTTCTAGAGTACCTCAGTGGATTGACTACTCCAATTCATTCACTTGCAAGTGGGGTGGGCTATTTAGAAGGTGTGATATGGGAGATTGTCCTCACATTCTCCTTGCTATTCACAGTCTATGCTACAATTGTCGACAAAAGAGGATCTCTTAATGGATTAGGCCCTACATTAACTGGATTTGTTGTCGGCGCCAACATCCTAGCTGGAGGAGCCTTCTCAGGAGCTTCAATGAACCCAGCGAGGTCCTTTGGTCCTGCTTTGGTAAGCTGGAATTGGACTGACCATTGGGTTTATTGGGTTGGTCCTCTGATTGGCGGTGGACTTGCTGGGTTCATTTATGAAAATTTCTTCATTATCGGGCAAGCTCATCTCCCAATTCCTACAGCGGAAGCTGCTTTGTTAGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005372 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006833 GO:0008150 GO:0009705 GO:0015250 GO:0015267 GO:0015318 GO:0015840 GO:0016020 GO:0016021 GO:0019755 GO:0022803 GO:0022838 GO:0022857 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042044 GO:0042807 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MIP

Protein Analysis

249

Amino Acids

25.86

Weight (kDa)

5.51

Isoelectric Point (pI)

21.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIP	PF00230	16 - 226	7.6e-66	Major intrinsic protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016674)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25810
fragaria_vesca	FvH4_2g35700
malus_domestica	MD08G1115300.v1.1 MD15G1094400.v1.1
prunus_persica	Prupe.1G449500_v2.0.a1
pyrus_communis	pycom15g08720
rosa_chinensis	RchiOBHm_Chr6g0306101
rosa_laevigata	RLG00000010825
rosa_rugosa	Rorug06G0346800
rosa_samantha	Rh6AG459100 Rh6BG441800 Rh6CG473600 Rh6DG459800
rosa_wichuraiana	Rw6G040020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	35, 527
AccI	GTMKAC	1 cut(s)	471
AciI	CCGC	4 cut(s)	207, 225, 654, 725
AclWI	GGATC	2 cut(s)	311, 490
AcoI	YGGCCR	2 cut(s)	192, 211
AcsI	RAATTY	1 cut(s)	682
AcyI	GRCGYC	1 cut(s)	528
AfaI	GTAC	1 cut(s)	350
AfiI	CCNNNNNNNGG	1 cut(s)	650
AgeI	ACCGGT	1 cut(s)	26
AgsI	TTSAA	1 cut(s)	567
AhlI	ACTAGT	2 cut(s)	148, 184
AjuI	GAANNNNNNNTTGG	1 cut(s)	31
Alw21I	GWGCWC	1 cut(s)	61
AlwI	GGATC	2 cut(s)	311, 490
AlwNI	CAGNNNCTG	1 cut(s)	326
AoxI	GGCC	4 cut(s)	192, 211, 253, 499
ApeKI	GCWGC	6 cut(s)	137, 320, 323, 326, 329, 731
ApoI	RAATTY	1 cut(s)	682
AsiGI	ACCGGT	1 cut(s)	26
AspLEI	GCGC	1 cut(s)	530
AspS9I	GGNCC	4 cut(s)	500, 582, 590, 641
AsuHPI	GGTGA	2 cut(s)	41, 221
AsuNHI	GCTAGC	1 cut(s)	133
AvaII	GGWCC	3 cut(s)	582, 590, 641
BaeGI	GKGCMC	1 cut(s)	40
BanI	GGYRCC	2 cut(s)	35, 527
BanII	GRGCYC	1 cut(s)	61
Bbv12I	GWGCWC	1 cut(s)	61
BbvI	GCAGC	6 cut(s)	149, 310, 313, 332, 341, 718
BceAI	ACGGC	3 cut(s)	149, 179, 227
BcuI	ACTAGT	2 cut(s)	148, 184
BfaI	CTAG	5 cut(s)	134, 149, 185, 344, 539
BfmI	CTRYAG	2 cut(s)	327, 720
BfoI	RGCGCY	1 cut(s)	531
BisI	GCNGC	6 cut(s)	138, 321, 324, 327, 330, 732
BlsI	GCNGC	6 cut(s)	139, 322, 325, 328, 331, 733
Bme18I	GGWCC	3 cut(s)	582, 590, 641
BmgT120I	GGNCC	4 cut(s)	500, 582, 590, 641
BmiI	GGNNCC	4 cut(s)	22, 37, 529, 550
BmsI	GCATC	2 cut(s)	119, 133
BmtI	GCTAGC	1 cut(s)	137
BpmI	CTGGAG	2 cut(s)	73, 564
Bsa29I	ATCGAT	1 cut(s)	306
BsaHI	GRCGYC	1 cut(s)	528
BsaJI	CCNNGG	2 cut(s)	15, 117
BsaWI	WCCGGW	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	1 cut(s)	650
Bse118I	RCCGGY	2 cut(s)	26, 209
Bse1I	ACTGG	2 cut(s)	305, 517
BseCI	ATCGAT	1 cut(s)	306
BseDI	CCNNGG	2 cut(s)	15, 117
BseGI	GGATG	1 cut(s)	534
BseLI	CCNNNNNNNGG	1 cut(s)	650
BseMII	CTCAG	2 cut(s)	367, 570
BseNI	ACTGG	2 cut(s)	305, 517
BseRI	GAGGAG	1 cut(s)	561
BseSI	GKGCMC	1 cut(s)	40
BseXI	GCAGC	6 cut(s)	149, 310, 313, 332, 341, 718
BseYI	CCCAGC	2 cut(s)	574, 665
BshFI	GGCC	4 cut(s)	194, 213, 255, 501
BshNI	GGYRCC	2 cut(s)	35, 527
BshTI	ACCGGT	1 cut(s)	26
BshVI	ATCGAT	1 cut(s)	306
BsiHKAI	GWGCWC	1 cut(s)	61
BsiSI	CCGG	2 cut(s)	27, 210
BslI	CCNNNNNNNGG	1 cut(s)	650
BsnI	GGCC	4 cut(s)	194, 213, 255, 501
Bsp1286I	GDGCHC	2 cut(s)	40, 61
Bsp143I	GATC	4 cut(s)	287, 303, 307, 482
Bsp19I	CCATGG	1 cut(s)	117
BspACI	CCGC	4 cut(s)	207, 225, 654, 725
BspANI	GGCC	4 cut(s)	194, 213, 255, 501
BspCNI	CTCAG	2 cut(s)	366, 569
BspDI	ATCGAT	1 cut(s)	306
BspLI	GGNNCC	4 cut(s)	22, 37, 529, 550
BspMAI	CTGCAG	1 cut(s)	331
BspOI	GCTAGC	1 cut(s)	137
BspPI	GGATC	2 cut(s)	311, 490
BspT107I	GGYRCC	2 cut(s)	35, 527
BsrFI	RCCGGY	2 cut(s)	26, 209
BsrI	ACTGG	2 cut(s)	305, 517
BssAI	RCCGGY	2 cut(s)	26, 209
BssECI	CCNNGG	2 cut(s)	15, 117
BssMI	GATC	4 cut(s)	287, 303, 307, 482
BssNI	GRCGYC	1 cut(s)	528
BssT1I	CCWWGG	2 cut(s)	15, 117
Bst4CI	ACNGT	1 cut(s)	454
Bst6I	CTCTTC	2 cut(s)	95, 287
BstACI	GRCGYC	1 cut(s)	528
BstAPI	GCANNNNNTGC	2 cut(s)	143, 326
BstC8I	GCNNGC	3 cut(s)	135, 211, 702
BstDEI	CTNAG	2 cut(s)	353, 556
BstDSI	CCRYGG	1 cut(s)	117
BstEII	GGTNACC	1 cut(s)	227
BstF5I	GGATG	1 cut(s)	534
BstH2I	RGCGCY	1 cut(s)	531
BstHHI	GCGC	1 cut(s)	530
BstKTI	GATC	4 cut(s)	290, 306, 310, 485
BstMBI	GATC	4 cut(s)	287, 303, 307, 482
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 107, 143, 326, 329, 548, 731
BstNSI	RCATGY	1 cut(s)	182
BstPI	GGTNACC	1 cut(s)	227
BstSFI	CTRYAG	2 cut(s)	327, 720
BstSLI	GKGCMC	1 cut(s)	40
BstV1I	GCAGC	6 cut(s)	149, 310, 313, 332, 341, 718
BstX2I	RGATCY	1 cut(s)	482
BstXI	CCANNNNNNTGG	2 cut(s)	51, 221
BstYI	RGATCY	1 cut(s)	482
Bsu15I	ATCGAT	1 cut(s)	306
BsuRI	GGCC	4 cut(s)	194, 213, 255, 501
BsuTUI	ATCGAT	1 cut(s)	306
BtgI	CCRYGG	1 cut(s)	117
BtsCI	GGATG	1 cut(s)	534
BtsIMutI	CAGTG	1 cut(s)	361
Cac8I	GCNNGC	3 cut(s)	135, 211, 702
CaiI	CAGNNNCTG	1 cut(s)	326
CfoI	GCGC	1 cut(s)	530
Cfr10I	RCCGGY	2 cut(s)	26, 209
Cfr13I	GGNCC	4 cut(s)	500, 582, 590, 641
ClaI	ATCGAT	1 cut(s)	306
Csp6I	GTAC	1 cut(s)	349
CspAI	ACCGGT	1 cut(s)	26
CspCI	CAANNNNNGTGG	2 cut(s)	375, 410
CviAII	CATG	2 cut(s)	118, 179
CviQI	GTAC	1 cut(s)	349
DdeI	CTNAG	2 cut(s)	353, 556
DinI	GGCGCC	1 cut(s)	529
DpnI	GATC	4 cut(s)	289, 305, 309, 484
DpnII	GATC	4 cut(s)	287, 303, 307, 482
EaeI	YGGCCR	2 cut(s)	192, 211
Eam1104I	CTCTTC	2 cut(s)	95, 287
EarI	CTCTTC	2 cut(s)	95, 287
EciI	GGCGGA	1 cut(s)	196
Ecl136II	GAGCTC	1 cut(s)	59
Eco130I	CCWWGG	2 cut(s)	15, 117
Eco24I	GRGCYC	1 cut(s)	61
Eco32I	GATATC	1 cut(s)	204
Eco47I	GGWCC	3 cut(s)	582, 590, 641
Eco53kI	GAGCTC	1 cut(s)	59
Eco91I	GGTNACC	1 cut(s)	227
EcoICRI	GAGCTC	1 cut(s)	59
EcoO109I	RGGNCCY	2 cut(s)	500, 582
EcoO65I	GGTNACC	1 cut(s)	227
EcoRV	GATATC	1 cut(s)	204
EcoT14I	CCWWGG	2 cut(s)	15, 117
EcoT38I	GRGCYC	1 cut(s)	61
EgeI	GGCGCC	1 cut(s)	529
EheI	GGCGCC	1 cut(s)	529
ErhI	CCWWGG	2 cut(s)	15, 117
FaeI	CATG	2 cut(s)	121, 182
FaiI	YATR	8 cut(s)	119, 180, 218, 273, 293, 418, 459, 678
FatI	CATG	2 cut(s)	117, 178
FblI	GTMKAC	1 cut(s)	471
Fnu4HI	GCNGC	6 cut(s)	138, 321, 324, 327, 330, 732
FokI	GGATG	1 cut(s)	521
FriOI	GRGCYC	1 cut(s)	61
Fsp4HI	GCNGC	6 cut(s)	138, 321, 324, 327, 330, 732
FspBI	CTAG	5 cut(s)	134, 149, 185, 344, 539
GlaI	GCGC	1 cut(s)	529
GluI	GCNGC	6 cut(s)	138, 321, 324, 327, 330, 732
GsaI	CCCAGC	2 cut(s)	578, 669
GsuI	CTGGAG	2 cut(s)	73, 564
HaeII	RGCGCY	1 cut(s)	531
HaeIII	GGCC	4 cut(s)	194, 213, 255, 501
HapII	CCGG	2 cut(s)	27, 210
HhaI	GCGC	1 cut(s)	530
Hin1I	GRCGYC	1 cut(s)	528
Hin1II	CATG	2 cut(s)	121, 182
Hin6I	GCGC	1 cut(s)	528
HinP1I	GCGC	1 cut(s)	528
HincII	GTYRAC	1 cut(s)	472
HindII	GTYRAC	1 cut(s)	472
HpaII	CCGG	2 cut(s)	27, 210
HphI	GGTGA	2 cut(s)	41, 221
Hpy166II	GTNNAC	2 cut(s)	472, 659
Hpy188I	TCNGA	2 cut(s)	287, 648
Hpy188III	TCNNGA	4 cut(s)	44, 52, 344, 558
Hpy8I	GTNNAC	2 cut(s)	472, 659
HpyAV	CCTTC	5 cut(s)	50, 94, 350, 401, 562
HpyCH4III	ACNGT	1 cut(s)	454
HpyCH4V	TGCA	5 cut(s)	110, 146, 182, 329, 386
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 107, 143, 326, 329, 548, 731
HpyF3I	CTNAG	2 cut(s)	353, 556
Hsp92I	GRCGYC	1 cut(s)	528
Hsp92II	CATG	2 cut(s)	121, 182
HspAI	GCGC	1 cut(s)	528
KasI	GGCGCC	1 cut(s)	527
KroI	GCCGGC	1 cut(s)	209
KroNI	GCCGGC	1 cut(s)	211
Kzo9I	GATC	4 cut(s)	287, 303, 307, 482
LmnI	GCTCC	2 cut(s)	548, 560
Lsp1109I	GCAGC	6 cut(s)	149, 310, 313, 332, 341, 718
LweI	GCATC	2 cut(s)	119, 133
MaeI	CTAG	5 cut(s)	134, 149, 185, 344, 539
MaeIII	GTNAC	4 cut(s)	172, 227, 244, 335
MalI	GATC	4 cut(s)	289, 305, 309, 484
MboI	GATC	4 cut(s)	287, 303, 307, 482
MboII	GAAGA	3 cut(s)	82, 274, 679
MfeI	CAATTG	2 cut(s)	311, 465
MflI	RGATCY	1 cut(s)	482
MhlI	GDGCHC	2 cut(s)	40, 61
MluCI	AATT	6 cut(s)	311, 372, 465, 610, 682, 714
Mly113I	GGCGCC	1 cut(s)	528
MroNI	GCCGGC	1 cut(s)	209
MseI	TTAA	4 cut(s)	234, 489, 509, 745
MspA1I	CMGCKG	2 cut(s)	323, 725
MspI	CCGG	2 cut(s)	27, 210
MunI	CAATTG	2 cut(s)	311, 465
MwoI	GCNNNNNNNGC	7 cut(s)	11, 107, 143, 326, 329, 548, 731
NaeI	GCCGGC	1 cut(s)	211
NarI	GGCGCC	1 cut(s)	528
NcoI	CCATGG	1 cut(s)	117
NdeII	GATC	4 cut(s)	287, 303, 307, 482
NgoMIV	GCCGGC	1 cut(s)	209
NheI	GCTAGC	1 cut(s)	133
NlaIII	CATG	2 cut(s)	121, 182
NlaIV	GGNNCC	4 cut(s)	22, 37, 529, 550
NmuCI	GTSAC	3 cut(s)	172, 227, 244
NspI	RCATGY	1 cut(s)	182
PdiI	GCCGGC	1 cut(s)	211
PinAI	ACCGGT	1 cut(s)	26
PkrI	GCNGC	6 cut(s)	139, 322, 325, 328, 331, 733
PluTI	GGCGCC	1 cut(s)	531
PpuMI	RGGWCCY	1 cut(s)	582
Psp124BI	GAGCTC	1 cut(s)	61
Psp5II	RGGWCCY	1 cut(s)	582
PspEI	GGTNACC	1 cut(s)	227
PspFI	CCCAGC	2 cut(s)	574, 665
PspN4I	GGNNCC	4 cut(s)	22, 37, 529, 550
PspPI	GGNCC	4 cut(s)	500, 582, 590, 641
PspPPI	RGGWCCY	1 cut(s)	582
PstI	CTGCAG	1 cut(s)	331
PstNI	CAGNNNCTG	1 cut(s)	326
PsuI	RGATCY	1 cut(s)	482
PvuII	CAGCTG	1 cut(s)	323
RsaI	GTAC	1 cut(s)	350
RsaNI	GTAC	1 cut(s)	349
SacI	GAGCTC	1 cut(s)	61
SalI	GTCGAC	1 cut(s)	470
SaqAI	TTAA	4 cut(s)	234, 489, 509, 745
SatI	GCNGC	6 cut(s)	138, 321, 324, 327, 330, 732
Sau3AI	GATC	4 cut(s)	287, 303, 307, 482
Sau96I	GGNCC	4 cut(s)	500, 582, 590, 641
SduI	GDGCHC	2 cut(s)	40, 61
SfaNI	GCATC	2 cut(s)	119, 133
SfcI	CTRYAG	2 cut(s)	327, 720
SfoI	GGCGCC	1 cut(s)	529
SgrAI	CRCCGGYG	1 cut(s)	26
SinI	GGWCC	3 cut(s)	582, 590, 641
SpeI	ACTAGT	2 cut(s)	148, 184
Sse9I	AATT	6 cut(s)	311, 372, 465, 610, 682, 714
SsiI	CCGC	4 cut(s)	207, 225, 654, 725
SspDI	GGCGCC	1 cut(s)	527
SspMI	CTAG	5 cut(s)	134, 149, 185, 344, 539
SstI	GAGCTC	1 cut(s)	61
StyI	CCWWGG	2 cut(s)	15, 117
TaaI	ACNGT	1 cut(s)	454
TaqI	TCGA	2 cut(s)	306, 471
TasI	AATT	6 cut(s)	311, 372, 465, 610, 682, 714
Tru1I	TTAA	4 cut(s)	234, 489, 509, 745
Tru9I	TTAA	4 cut(s)	234, 489, 509, 745
TscAI	CASTG	1 cut(s)	361
TseFI	GTSAC	3 cut(s)	172, 227, 244
TseI	GCWGC	6 cut(s)	137, 320, 323, 326, 329, 731
Tsp45I	GTSAC	3 cut(s)	172, 227, 244
TspDTI	ATGAA	5 cut(s)	365, 584, 661, 679, 693
TspRI	CASTG	1 cut(s)	361
VpaK11BI	GGWCC	3 cut(s)	582, 590, 641
XapI	RAATTY	1 cut(s)	682
XbaI	TCTAGA	1 cut(s)	343
XceI	RCATGY	1 cut(s)	182
XmiI	GTMKAC	1 cut(s)	471
XspI	CTAG	5 cut(s)	134, 149, 185, 344, 539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.