AT2G25910

3'-5' exonuclease domain-containing protein K homology domain-containing protein KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0016652
Physical Location & Seq
Reverse (-)
11048946 .. 11052075
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25910.1

Sequence Viewer

Length: 1026 bp
ATGGCGTCGTCTTCTCCAACTCAGCTAGCTCACGTCCCGATTCCTCCAGAACCAGGAGGAAGGTCGCCGACACAAGAAGCTAATGAGCCACCAGTTCCGATTTACATTGTGACGGATCCGTTTCAACTTCCTGCTGATTTCCTAAACCCTTCTCCTGAAAAGAAATTGGTTATCGGTTTTGACTGTGAGGGTGTTGACCTCTGCCGACATGGGAAACTTTGTATCATGCAGATTGCATTCTCTAATGCAATATACTTGGTTGATGTCATCGAAGGTGGAGAGGTGATTATGAAAGCGTGTAAGCCTGCACTCGAGTCTAATTACATCACGAAAGTTATTCACGATTGCAAGCGTGACAGTGAGGCTCTATACTTCCAGTTTGGGATAAGATTGCACAATGTTGTGGACACTCAGATTGCTTATTCTCTGATTGAAGAACAAGAAGGGCGGAGGAGACCTCTAGATGATTACATATCGTTTGTTTCACTCCTTGCTGATCCACGTTACTGCGGTATATCCTATGAAGAGAAAGAAGAAGTTCGAGTTCTCATGCGCCAGGACCCAAAGTTTTGGACATACAGGCCTATGACTGAGCTCATGATCCGCGCAGCTGCTGATGATGTCCGCTTCCTTCTGTATCTCTATCACAAAATGATGGGAAAGCTAAATCAGCGGTCACTATGGCATCTTGCAGTTCGTGGTGCTTTGTACTGTCGGTGTCTCTGCTGCATGAATGATGCTGATTTTGCTGATTGGCCAACCGTTCCTCCAATTCCAGATAACCTGAAGTCAGAAGATCAATGTCTTGAAGAAGAGATCCTGTCAGTGCTTGATGTTCCACCAGGAAAGATGGGACGTGTGATTGGAAGGAAAGGAGCATCGATCCTCGCCATTAAGGAAGCTTGCAACGCGGAAATTCTAATTGGAGGGGCAAAGGGTCCACCTGATAAGATATTTGTGATTGGACCGGTGAGAGAAGTGCGTAAGGCGGAGGCTATACTGAGAGGAAGAATGATAGACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_A_exo1 KH_1

Protein Analysis

341

Amino Acids

38.39

Weight (kDa)

5.53

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 48 - 222 2.1e-24 3'-5' exonuclease
KH_1 PF00013 277 - 334 5.9e-09 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016652)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25910 AT2G25910
fragaria_vesca FvH4_2g32310
malus_domestica MD08G1074400.v1.1
prunus_persica Prupe.1G416100_v2.0.a1 Prupe.1G416100_v2.0.a1
pyrus_communis pycom08g06140
rosa_chinensis RchiOBHm_Chr6g0310381
rosa_laevigata RLG00000010487
rosa_roxburghii Rroxscaffold_7G00158600
rosa_rugosa Rorug06G0379600
rosa_samantha Rh6BG503400 Rh6CG508400
rosa_wichuraiana Rw6G042960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 606, 911
AciI CCGC 7 cut(s) 448, 510, 604, 625, 673, 911, 989
AclWI GGATC 6 cut(s) 110, 123, 491, 595, 811, 877
AcoI YGGCCR 1 cut(s) 755
AcsI RAATTY 1 cut(s) 915
AcuI CTGAAG 1 cut(s) 806
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 710
AfiI CCNNNNNNNGG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 856
AgeI ACCGGT 1 cut(s) 967
AgsI TTSAA 3 cut(s) 125, 434, 809
AjiI CACGTC 2 cut(s) 34, 857
AjnI CCWGG 3 cut(s) 52, 555, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 906, 938
AluBI AGCT 7 cut(s) 25, 29, 80, 595, 611, 664, 902
AluI AGCT 7 cut(s) 25, 29, 80, 595, 611, 664, 902
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 2 cut(s) 448, 725
AlwI GGATC 6 cut(s) 110, 123, 491, 595, 811, 877
AlwNI CAGNNNCTG 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 2 cut(s) 581, 755
ApeKI GCWGC 3 cut(s) 608, 611, 726
ApoI RAATTY 1 cut(s) 915
AsiGI ACCGGT 1 cut(s) 967
Asp700I GAANNNNTTC 1 cut(s) 537
AspLEI GCGC 2 cut(s) 555, 608
AspS9I GGNCC 3 cut(s) 559, 938, 965
AsuHPI GGTGA 2 cut(s) 295, 982
AsuNHI GCTAGC 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 3 cut(s) 559, 938, 965
BalI TGGCCA 1 cut(s) 757
BamHI GGATCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 597
BbsI GAAGAC 1 cut(s) 3
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 3 cut(s) 598, 620, 713
BccI CCATC 2 cut(s) 649, 844
BciT130I CCWGG 3 cut(s) 54, 557, 843
BcoDI GTCTC 2 cut(s) 448, 725
BfaI CTAG 2 cut(s) 26, 461
BisI GCNGC 3 cut(s) 609, 612, 727
BlsI GCNGC 3 cut(s) 610, 613, 728
Bme1390I CCNGG 3 cut(s) 54, 557, 843
Bme18I GGWCC 3 cut(s) 559, 938, 965
BmeT110I CYCGRG 1 cut(s) 311
BmgBI CACGTC 2 cut(s) 34, 857
BmgT120I GGNCC 3 cut(s) 559, 938, 965
BmiI GGNNCC 3 cut(s) 117, 561, 939
BmrFI CCNGG 3 cut(s) 54, 557, 843
BmsI GCATC 3 cut(s) 694, 727, 887
BmtI GCTAGC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 3
BplI GAGNNNNNCTC 2 cut(s) 442, 474
BpmI CTGGAG 1 cut(s) 30
Bsa29I ATCGAT 1 cut(s) 881
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 448
BsaWI WCCGGW 1 cut(s) 967
BsaXI ACNNNNNCTCC 2 cut(s) 751, 781
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse118I RCCGGY 1 cut(s) 967
Bse1I ACTGG 2 cut(s) 92, 376
BseBI CCWGG 3 cut(s) 54, 557, 843
BseCI ATCGAT 1 cut(s) 881
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMII CTCAG 4 cut(s) 35, 425, 582, 992
BseNI ACTGG 2 cut(s) 92, 376
BseRI GAGGAG 1 cut(s) 466
BseXI GCAGC 3 cut(s) 598, 620, 713
BsgI GTGCAG 1 cut(s) 291
Bsh1236I CGCG 2 cut(s) 606, 911
BshFI GGCC 2 cut(s) 583, 757
BshTI ACCGGT 1 cut(s) 967
BshVI ATCGAT 1 cut(s) 881
BsiHKAI GWGCWC 1 cut(s) 597
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 1 cut(s) 968
BslFI GGGAC 2 cut(s) 20, 867
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 2 cut(s) 448, 725
BsmFI GGGAC 2 cut(s) 20, 867
BsmI GAATGC 1 cut(s) 236
BsnI GGCC 2 cut(s) 583, 757
Bso31I GGTCTC 1 cut(s) 448
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 597
Bsp143I GATC 6 cut(s) 115, 496, 600, 796, 816, 882
BspACI CCGC 7 cut(s) 448, 510, 604, 625, 673, 911, 989
BspANI GGCC 2 cut(s) 583, 757
BspCNI CTCAG 4 cut(s) 34, 424, 583, 993
BspDI ATCGAT 1 cut(s) 881
BspFNI CGCG 2 cut(s) 606, 911
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 3 cut(s) 117, 561, 939
BspOI GCTAGC 1 cut(s) 29
BspPI GGATC 6 cut(s) 110, 123, 491, 595, 811, 877
BspTNI GGTCTC 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 967
BsrI ACTGG 2 cut(s) 92, 376
BssAI RCCGGY 1 cut(s) 967
BssMI GATC 6 cut(s) 115, 496, 600, 796, 816, 882
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 3 cut(s) 54, 557, 843
Bst4CI ACNGT 4 cut(s) 185, 359, 713, 763
Bst6I CTCTTC 2 cut(s) 519, 807
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 4 cut(s) 27, 306, 350, 904
BstDEI CTNAG 4 cut(s) 21, 411, 591, 1001
BstFNI CGCG 2 cut(s) 606, 911
BstHHI GCGC 2 cut(s) 555, 608
BstKTI GATC 6 cut(s) 118, 499, 603, 799, 819, 885
BstMAI GTCTC 2 cut(s) 448, 725
BstMBI GATC 6 cut(s) 115, 496, 600, 796, 816, 882
BstMWI GCNNNNNNNGC 3 cut(s) 670, 746, 908
BstNI CCWGG 3 cut(s) 54, 557, 843
BstSCI CCNGG 3 cut(s) 52, 555, 841
BstUI CGCG 2 cut(s) 606, 911
BstV1I GCAGC 3 cut(s) 598, 620, 713
BstV2I GAAGAC 1 cut(s) 3
BstX2I RGATCY 2 cut(s) 115, 816
BstXI CCANNNNNNTGG 1 cut(s) 570
BstYI RGATCY 2 cut(s) 115, 816
Bsu15I ATCGAT 1 cut(s) 881
BsuRI GGCC 2 cut(s) 583, 757
BsuTUI ATCGAT 1 cut(s) 881
BtrI CACGTC 2 cut(s) 34, 857
BtsIMutI CAGTG 2 cut(s) 364, 831
Cac8I GCNNGC 4 cut(s) 27, 306, 350, 904
CaiI CAGNNNCTG 1 cut(s) 614
CciI TCATGA 1 cut(s) 597
CfoI GCGC 2 cut(s) 555, 608
Cfr10I RCCGGY 1 cut(s) 967
Cfr13I GGNCC 3 cut(s) 559, 938, 965
ClaI ATCGAT 1 cut(s) 881
Csp6I GTAC 1 cut(s) 709
CspAI ACCGGT 1 cut(s) 967
CviAII CATG 5 cut(s) 209, 226, 550, 598, 730
CviQI GTAC 1 cut(s) 709
DdeI CTNAG 4 cut(s) 21, 411, 591, 1001
DpnI GATC 6 cut(s) 117, 498, 602, 798, 818, 884
DpnII GATC 6 cut(s) 115, 496, 600, 796, 816, 882
EaeI YGGCCR 1 cut(s) 755
Eam1104I CTCTTC 2 cut(s) 519, 807
EarI CTCTTC 2 cut(s) 519, 807
EciI GGCGGA 2 cut(s) 463, 1004
Ecl136II GAGCTC 1 cut(s) 595
Eco147I AGGCCT 1 cut(s) 583
Eco24I GRGCYC 1 cut(s) 597
Eco31I GGTCTC 1 cut(s) 448
Eco47I GGWCC 3 cut(s) 559, 938, 965
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 806
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 595
EcoO109I RGGNCCY 1 cut(s) 559
EcoRII CCWGG 3 cut(s) 52, 555, 841
EcoT38I GRGCYC 1 cut(s) 597
FaeI CATG 5 cut(s) 212, 229, 553, 601, 733
FaqI GGGAC 2 cut(s) 20, 867
FatI CATG 5 cut(s) 208, 225, 549, 597, 729
Fnu4HI GCNGC 3 cut(s) 609, 612, 727
FriOI GRGCYC 1 cut(s) 597
Fsp4HI GCNGC 3 cut(s) 609, 612, 727
FspBI CTAG 2 cut(s) 26, 461
GlaI GCGC 2 cut(s) 554, 607
GluI GCNGC 3 cut(s) 609, 612, 727
GsuI CTGGAG 1 cut(s) 30
HaeIII GGCC 2 cut(s) 583, 757
HapII CCGG 1 cut(s) 968
HhaI GCGC 2 cut(s) 555, 608
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 212, 229, 553, 601, 733
Hin6I GCGC 2 cut(s) 553, 606
HinP1I GCGC 2 cut(s) 553, 606
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 900
HinfI GANTC 2 cut(s) 40, 314
HpaII CCGG 1 cut(s) 968
HphI GGTGA 2 cut(s) 295, 982
Hpy166II GTNNAC 3 cut(s) 196, 406, 941
Hpy188I TCNGA 4 cut(s) 99, 414, 429, 793
Hpy188III TCNNGA 9 cut(s) 37, 47, 155, 328, 341, 461, 598, 776, 806
Hpy8I GTNNAC 3 cut(s) 196, 406, 941
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 6 cut(s) 54, 159, 266, 437, 641, 861
HpyCH4III ACNGT 4 cut(s) 185, 359, 713, 763
HpyCH4IV ACGT 3 cut(s) 33, 502, 856
HpyCH4V TGCA 9 cut(s) 229, 236, 248, 308, 348, 394, 692, 729, 906
HpyF10VI GCNNNNNNNGC 3 cut(s) 670, 746, 908
HpyF3I CTNAG 4 cut(s) 21, 411, 591, 1001
HpySE526I ACGT 3 cut(s) 33, 502, 856
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 212, 229, 553, 601, 733
HspAI GCGC 2 cut(s) 553, 606
Kzo9I GATC 6 cut(s) 115, 496, 600, 796, 816, 882
LmnI GCTCC 1 cut(s) 875
Lsp1109I GCAGC 3 cut(s) 598, 620, 713
LweI GCATC 3 cut(s) 694, 727, 887
MaeI CTAG 2 cut(s) 26, 461
MaeII ACGT 3 cut(s) 33, 502, 856
MaeIII GTNAC 4 cut(s) 109, 353, 503, 675
MalI GATC 6 cut(s) 117, 498, 602, 798, 818, 884
MboI GATC 6 cut(s) 115, 496, 600, 796, 816, 882
MboII GAAGA 8 cut(s) 3, 446, 536, 545, 806, 821, 824, 1020
MflI RGATCY 2 cut(s) 115, 816
MhlI GDGCHC 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 757
MluCI AATT 5 cut(s) 164, 319, 771, 915, 921
MluNI TGGCCA 1 cut(s) 757
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 41
Mox20I TGGCCA 1 cut(s) 757
MroXI GAANNNNTTC 1 cut(s) 537
MscI TGGCCA 1 cut(s) 757
MseI TTAA 1 cut(s) 894
Msp20I TGGCCA 1 cut(s) 757
MspA1I CMGCKG 2 cut(s) 611, 673
MspI CCGG 1 cut(s) 968
MspR9I CCNGG 3 cut(s) 54, 557, 843
Mva1269I GAATGC 1 cut(s) 236
MvaI CCWGG 3 cut(s) 54, 557, 843
MvnI CGCG 2 cut(s) 606, 911
MwoI GCNNNNNNNGC 3 cut(s) 670, 746, 908
NdeII GATC 6 cut(s) 115, 496, 600, 796, 816, 882
NheI GCTAGC 1 cut(s) 25
NlaIII CATG 5 cut(s) 212, 229, 553, 601, 733
NlaIV GGNNCC 3 cut(s) 117, 561, 939
NmuCI GTSAC 3 cut(s) 109, 353, 675
PaeR7I CTCGAG 1 cut(s) 311
PagI TCATGA 1 cut(s) 597
PceI AGGCCT 1 cut(s) 583
PctI GAATGC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 537
PfeI GAWTC 1 cut(s) 40
PinAI ACCGGT 1 cut(s) 967
PkrI GCNGC 3 cut(s) 610, 613, 728
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 559
Psp124BI GAGCTC 1 cut(s) 597
Psp5II RGGWCCY 1 cut(s) 559
Psp6I CCWGG 3 cut(s) 52, 555, 841
PspGI CCWGG 3 cut(s) 52, 555, 841
PspN4I GGNNCC 3 cut(s) 117, 561, 939
PspPI GGNCC 3 cut(s) 559, 938, 965
PspPPI RGGWCCY 1 cut(s) 559
PspXI VCTCGAGB 1 cut(s) 311
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 2 cut(s) 115, 816
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 1 cut(s) 710
RsaNI GTAC 1 cut(s) 709
SacI GAGCTC 1 cut(s) 597
SaqAI TTAA 1 cut(s) 894
SatI GCNGC 3 cut(s) 609, 612, 727
Sau3AI GATC 6 cut(s) 115, 496, 600, 796, 816, 882
Sau96I GGNCC 3 cut(s) 559, 938, 965
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 3 cut(s) 54, 557, 843
SduI GDGCHC 1 cut(s) 597
SfaNI GCATC 3 cut(s) 694, 727, 887
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 3 cut(s) 559, 938, 965
SlaI CTCGAG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 5 cut(s) 164, 319, 771, 915, 921
SseBI AGGCCT 1 cut(s) 583
SsiI CCGC 7 cut(s) 448, 510, 604, 625, 673, 911, 989
SspMI CTAG 2 cut(s) 26, 461
SstI GAGCTC 1 cut(s) 597
StuI AGGCCT 1 cut(s) 583
StyD4I CCNGG 3 cut(s) 52, 555, 841
TaaI ACNGT 4 cut(s) 185, 359, 713, 763
TaiI ACGT 3 cut(s) 36, 505, 859
TaqI TCGA 4 cut(s) 270, 312, 541, 881
TasI AATT 5 cut(s) 164, 319, 771, 915, 921
TatI WGTACW 1 cut(s) 708
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 1 cut(s) 894
Tru9I TTAA 1 cut(s) 894
TscAI CASTG 2 cut(s) 364, 831
TseFI GTSAC 3 cut(s) 109, 353, 675
TseI GCWGC 3 cut(s) 608, 611, 726
Tsp45I GTSAC 3 cut(s) 109, 353, 675
TspDTI ATGAA 3 cut(s) 305, 537, 746
TspGWI ACGGA 2 cut(s) 108, 128
TspRI CASTG 2 cut(s) 364, 831
VpaK11BI GGWCC 3 cut(s) 559, 938, 965
XapI RAATTY 1 cut(s) 915
XbaI TCTAGA 1 cut(s) 460
XhoI CTCGAG 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 537
XspI CTAG 2 cut(s) 26, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.