AT2G27120

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0019564
Physical Location & Seq
Forward (+)
11581214 .. 11594397
13184 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27120.1

Sequence Viewer

Length: 6417 bp
ATGAGCGGAAGGAGATGTGATCGGAGGTTGAATGTGCAGAAAGTTTCAGCGGCAGATGAACTCGAAACTAAACTTGGATTTGGGCTTTTCTCCCAAGGGGAAACGCGGCTCGGGTGGCTACTCACGTTTGCATCATCATCTTGGGAAGATGCCGATACTGGGAAAACCTTTAGTTGTGTGGACCTTTTCTTTGTCACTCAGGACGGCTCTTCTTTTAAGACTAAGTACAAGTTCCGCCCTTACTTGTATGCCGCCACCAAAGACAATATGGAACTGGAAGTTGAAGCCTACTTGAGACGCCGATATGAAAGACAAGTTGCTGATATTCAGATTGTTCACAAAGAAGATCTTTATCTTAAAAATCATCTATCTGGCCTGCAAAAAAAATATCTCAAAGTATCATTTGACACTGTGCAACAATTGGTGGAAGTAAAGAGGGATCTGTTGCATATTGTCGAACGAAACCTCGCAAAATTTAATGCACTTGAAGCTTACGAATCCATCTTGTCTGGAAAACGAGAACAACGTCCTCAAGACTGCTTAGACTCTGTGGTGGACCTCCGCGAGTACGATGTTCCTTATCATGTCCGATTTGCAATTGATAATGATGTGCGTAGTGGGCAATGGTACAATGTCAGTATTTCCAGCACTGATGTCATACTAGAGAAGAGGACGGATCTTCTCCAACGCGCAGAAGTTCGCGTTTGTGCATTCGATATAGAGACAGTAAAGCTTCCATTGAAGTTCCCGGATGCTGAATATGATCAAATTATGATGATATCCTACATGGTTGATGGGCAAGGTTTTCTAATTACTAACAGAGAGTGTGTTGGCAAAGACATAGAAGATCTGGAATATACACCCAAACCAGAGTTTGAAGGGTATTTCAAAGTCACGAATGTTACCAACGAGGTTGAACTCCTCCGAAAATGGTTTTCTCATATGCAAGAGTTGAAACCTGGTATTTATGTTACATACAACGGTGACTTTTTTGACTGGCCATTTATAGAACGGAGAGCATCTCATCATGGGATCAAAATGAACGAGGAGTTGGGATTCCGTTGTGATCAAAATCAAGGTGAATGTCGAGCTAAGTTTGTGTGCCATTTGGATTGTTTTTCTTGGGTGAAACGTGATAGCTATCTTCCTCAGGGAAGCCAGGGTCTGAAGGCTGTTACAAAGGTAAAACTGGGTTACGATCCACTGGAAGTGAATCCTGAGGACATGGTTCGGTTTGCAATGGAAAAGCCACAGACAATGGCCTCCTACTCTGTCTCTGATGCTGTTGCAACTTACTACCTATACATGACATATGTCCATCCGTTCGTTTTTTCTCTCGCAACTATCATTCCAATGGTCCCTGATGAGGTTTTACGCAAAGGGAGTGGCACCCTTTGTGAAATGCTTTTGATGGTTGAGGCTTACAAGGCAAATGTTGTATGTCCCAACAAAAATCAAGCTGACCCTGAGAAGTTCTACCAAGGTAAACTTCTTGAAAGTGAGACATATATTGGTGGCCATGTAGAGTGCCTCCAAAGTGGTGTTTTCAGATCTGACATTCCAACCAGTTTTAAGCTCGACGCATCCGCATACCAGCAACTGATCGATAACCTTGGTCGGGATCTGGAATATGCTATCACTGTTGAAGGAAAAATGAGAATGGATTCAGTTTCCAATTTTGATGAAGTAAAGGAAGTAATCAGGGAAAAGCTCGAGAAGTTACGGGATGATCCTATACGTGAAGAGGGGCCTCTTATTTATCACCTTGATGTCGCGGCGATGTATCCAAATATTATCTTAACAAACAGGCTTCAGCCACCATCAATAGTTACGGATGAGGTTTGCACAGCATGTGATTTCAACGGCCCGGAAAAGACCTGTCTTAGAAAACTTGAATGGGTTTGGCGTGGGGTGACGTTCAAGGGAAATAAAAGTGAATATTACCATCTGAAGAAGCAGATTGAGTCTGAATCTGTTGATGCTGGAGCAAATATGCAGTCTTCAAAACCTTTTCTTGATCTGCCAAAGGTGGAACAACAATCCAAATTAAAAGAACGACTGAAAAAATACTGTCAAAAGGCATATAGCCGAGTACTTGACAAGCCAATCACTGAAGTTCGCGAGGCTGGGATATGCATGAGAGAAAACCCGTTCTATGTGGACACTGTGCGCAGCTTTCGAGATAGGAGGTATGAATACAAAACACTTAACAAGGTCTGGAAGGGAAAGTTGTCAGAGGCCAAGGCAAGTGGCAATTTAATCAAGATCCAGGAAGCACATGACATGGTAGTGGTTTACGATTCCTTGCAGCTAGCTCATAAGTGTATACTTAATTCCTTTTATGGATATGTCATGCGAAAGGGCGCAAGATGGTACTCCATGGAAATGGCTGGGGTAGTTACTTATACTGGAGCCAAAATCATCCAGAATGCTCGTTTGCTTATTGAACGAATTGGGAAACCACTTGAGCTGGATACAGATGGTATATGGTGTGCTCTACCTGGATCTTTTCCTGAGAACTTTACCTTTAAAACCATAGATATGAAGAAATTCACCATCTCGTATCCGTGTGTAATACTTAATGTTGATGTGGCGAAGAATAATTCAAATGACCAATATCAGACTCTAGTAGATCCTGTACGAAAGACATATAATTCACGTAGCGAATGCTCAATTGAATTTGAAGTGGATGGACCGTACAAGGCAATGATTATTCCTGCATCCAAGGAAGAGGGAATCTTAATTAAGAAGCGGTATGCTGTTTTCAATCATGACGGAACCATAGCAGAACTCAAGGGGTTTGAGATGAAGCGTAGAGGAGAGCTGAAACTCATTAAAGTTTTCCAGGCCGAGCTATTTGACAAATTTCTGCATGGATCAACCCTTGAAGAGTGTTATTCTGCTGTTGCAGCTGTTGCAAATCGTTGGCTTGACCTACTCGAGGGTCAAGGAAAAGATATTGCCGATAGTGAATTGCTAGATTATATATCAGAATCAAGCACTATGAGCAAATCGTTAGCAGATTATGGTCAACAAAAGTCATGTGCAGTGACCACAGCAAAACGCCTGGCTGATTTTCTTGGTGATACAATGGTCAAAGATAAAGGACTGCGCTGCCAATACATCGTTGCTCGTGAACCAGAGGGGACACCTGTAAGTGAACGTGCTGTCCCTGTTGCCATATTTCAAACAGATGATCCTGAAAAAAAGTTTTATCTGCAAAAATGGTGCAAGATATCATCATATACTGGAATCCGTTCAATTATTGACTGGATGTATTACAAGCAGCGTCTTCATTCAGCTATTCAAAAAGTTATTACCATTCCTGCTGCAATGCAGAAGGTGGCAAATCCTGTACTTAGGGTGCGTCATCCTTATTGGCTCGAGAAAAAGGTCTGTGACAAATTTCGTCAGGGAAAAATAGTTGATATGTTTAGCTCAGCAAACAAAGATCATTCGACAACTCAAGATAATGTAGTGGCGGATATTGAAGAGTTTTGCAAAGAAAACAGACCTTCCGTAAAAGGGCCAAAGCCAGTTGCGCGTTCATTTGAAGTCGATAGAAATCACTCTGAGGGTAAGCAGCAAGAGAGTTGGGATCCAGAGTTCCATGATATCTCATTACAGAACGTTGACAAGAATGTAGATTACCAAGGATGGCTTGAACTGGAAAAAAGAAAATGGAAAATGACTCTAACGAATAAGAAAAAAAGAAGGTACTCCTCAAGTTTATTTGGTTTTGATTTGGAACAAAATATAAATAAAAAAGTCTGTAAGGGAAGAGTAGGTGTAGGCTCATACTTTAGGAGGCCTGAAGAAGCTTTGACTAGTTCCTATTTGCAGATAATACAGTTGGTCCAAAGTCCACAGAGCGGGCAATTTTTTGCTTGGGTTGTTGTGGAAGGATTGATGCTTAAGATCCCATTGACGATCCCAAGGGTGTTTTACATTAATTCCAAAGCTTCTATAGCTGGAAACTTCACGGGAAAGTGTATCAACAAGATTCTTCCTCACGGAAAGCCTTGTTACAATTTGATGGAGGTCAATATTCAGGAAGATCAATTTATAAAAGAAAGCAAAAAGCTTGCCGCTCTACTTGCAGACCCCGAAATTGAGGGCATATATGAAACTAAGATGCCTCTGGAGTTTAGTGCTATATGTCAGATTGGATGTGTATGCAAAATTGAAGACACGGCCAAACACCGTAACACCCAAGATGGATGGAAACTTGGTGAACTTCACAGGATAACTACAACTGAGTGTCGCTACTTGGAGAATTCTATTCCACTTGTTTATTTGTATCACAGCACCTCAACAGGTCGTGCTGTTTATGTTTTATATTGTCATGCTTCGAAGCTTATGTCTGTTGTGGTAGTCAATCCTTACGGTGACAAGGAATTATTATCATCTGCCCTAGAGAGACAGTTTAGAGATAGATGCCAAGAACTGTCACCTGAGCCATTTTCTTGGGATGGTATCCTTTTCCAGGTCGAGTATGTTGACCATCCAGAAGCTGCCACGAAATTTTTACAAAAAGCGCTCTGTGAATACAGAGAAGAAAATTGTGGAGCAACTGTGGCAGTCATTGAATGCCCTGACTTTAACACCACGAAGGAAGGTGTAAAGGCCCTGGAAGATTTCCCATGTGTGAGGATTCCCTTCAACGACGATGACAATAGTTATCAGCCTGTCTCCTGGCAACGTCCAGCAGCAAAAATAGCAGTGCTCCGTTGTGCTTCAGCAATTCAGTGGTTGGACCGGAGGATTGCCCAGTCAAGATACGCCCATGTGCCTCTGGGGAATTTTGGACGTGATTGGTTAACGTTCACAGTAGATATCTTCCTGTCCAGGGCACTCCGTGACCAGCAACAGGTTCTTTGGGTCTCAGATAATGGTGTTCCAGACCTTGGAGATATAAACAATGAAGAGACATTTCTAGCTGATGAGACAAGTTTGCTATTCCCTGGAGCGTACAGAAAAGTGTCTGTGGAGTTAAAGGTCCATAGATTAGCTGTTAATGCCCTTCTGAAAAGTGACCTGGTGAGTGAAATGGAAGGAGGCGGCTTTTTGGGTGTGAATTCTAGAGGAAGTAGCTTAAACGATAATGGCAGTTTTGATGAGAATAATGGATGCGCGCAAGCATTTCGTGTCCTGAAACAATTGATCAAGCGCTTGTTGCATGATGCGTGTAATTCTGGCAATATATATGCTGATTCCATTTTGCAACATCTGTCTTGGTGGCTCCGCAGCCCTTCCTCAAAGCTTCACGATCCAGCTCTCCATCTCATGCTTCACAAGGTCATGCAAAAGGTGTTTGCGCTGCTTTTGACTGATTTGCGGAGGTTAGGTGCTATAATAATATACGCAGACTTCTCAAAGGTCATTATCGACACAGGGAAATTTGATCTATCTGCTGCAAAGACTTATTGTGAAAGCTTGCTCACAGTGATGGGGAGTAGAGATATCTTTAAGTTGATCTTGCTTGAGCCGGTTCACTACTGGCACTCGTTACTTTTCATGGACCAGCATAACTACGCTGGTATCCGAGCTACCGGAGATGAAATTTCAGGTAACGAAGTTACTATTGAACCAAAGTGGAGCGTTGCACGGCATTTACCCGAGTATATTCAGAAGGATTTTATCATAATTGTTGCCACGTTTATATTTGGCCCCTGGAAATTTGCATTAGAGAAAAAAAGGGGCAGTGCAGAGAGTTTAGAGGCAGAGATGGTAGAATATCTCAAGGAACAGATAGGAACCCGGTTCATAAGCATGATTGTTGAGAAGATTGGTAATATCAGGTCACACATCAAGGATATAAACGTGTCAGATGCATCTTGGGCTTCTGGTCAAGCTCCCAAAGGAGATTACACGTTCGAATTTATACAAATTATTACTGCTGTTCTGGCTCTTGATCAAAACGTTCAGCAAGATGTTCTGGTAATGAGAAAGATTCTGTTGAAGTACATTAAAGTAAAAGAATGTGCTGCTGAAGCCGAATTTATTGATCCTGGGCCATCCTTCATCTTACCCAACGTGGCTTGCAGCAACTGCGGTGCCTACAGAGACCTAGATTTCTGCAGAGACTCAGCTCTCTTAACAGAAAAAGAATGGTCATGTGCGGATCCGCAATGTGTGAAGATATATGACAAAGAGCAGATAGAGAGTAGCATTATCCAGATGGTGAGACAGAGGGAAAGAATGTACCAGTTGCAGGATCTAGTGTGCAATAGGTGCAATCAGGTGAAAGCCGCTCATTTGACAGAGCAGTGTGAGTGTTCTGGATCCTTTAGATGCAAGGAGAGTGGTTCAGATTTCCACAAGAGGATTGAGATTTTCTTGGATATAGCAAAGCGCCAGAAGTTTAGACTGCTAGAAGAATGCATCTCCTGGATTTTATTTGCCACCAGCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000278 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009987 GO:0010015 GO:0010086 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022616 GO:0022622 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045004 GO:0045005 GO:0046483 GO:0048046 GO:0048364 GO:0048519 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048598 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051302 GO:0051716 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0090305 GO:0097159 GO:0099402 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1905392 GO:1990234 GO:2000026 GO:2000028 GO:2000241 GO:2000242
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

2138

Amino Acids

244.99

Weight (kDa)

6.49

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 186 - 392 6.7e-62 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 735 - 1064 9.3e-17 DNA polymerase family B
POL2_thumb PF22634 938 - 1113 1.2e-76 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 1492 - 1848 1.5e-103 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 1998 - 2065 1.6e-18 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 2070 - 2127 2.8e-15 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4067
Acc16I TGCGCA 1 cut(s) 2171
AccB1I GGYRCC 2 cut(s) 1388, 6056
AccB7I CCANNNNNTGG 1 cut(s) 4907
AccBSI CCGCTC 4 cut(s) 6, 3873, 4091, 6254
AccI GTMKAC 1 cut(s) 2326
AccII CGCG 8 cut(s) 106, 564, 690, 702, 1775, 2121, 3544, 5135
AclI AACGTT 3 cut(s) 3630, 4823, 5922
AcoI YGGCCR 3 cut(s) 998, 1516, 4194
AcuI CTGAAG 7 cut(s) 1187, 1796, 1970, 2133, 3834, 4722, 6012
AcyI GRCGYC 1 cut(s) 298
AdeI CACNNNGTG 1 cut(s) 4859
AfeI AGCGCT 2 cut(s) 4539, 5171
AflII CTTAAG 1 cut(s) 3914
AflIII ACRYGT 2 cut(s) 5823, 5871
AhlI ACTAGT 1 cut(s) 3827
AjiI CACGTC 1 cut(s) 4811
AjuI GAANNNNNNNTTGG 6 cut(s) 57, 89, 1034, 1066, 1473, 1505
AloI GAACNNNNNNTCC 4 cut(s) 3153, 3185, 3590, 3622
Alw21I GWGCWC 2 cut(s) 2497, 4728
AlwNI CAGNNNCTG 4 cut(s) 1165, 1600, 4514, 6051
Ama87I CYCGRG 5 cut(s) 110, 1712, 2939, 3383, 5618
Aor51HI AGCGCT 2 cut(s) 4539, 5171
ArsI GACNNNNNNTTYG 6 cut(s) 173, 205, 1982, 2014, 4346, 4378
AseI ATTAAT 1 cut(s) 3951
Asp700I GAANNNNTTC 3 cut(s) 1663, 2549, 3256
AsuC2I CCSGG 3 cut(s) 749, 1868, 5761
AsuII TTCGAA 2 cut(s) 4352, 5877
AsuNHI GCTAGC 1 cut(s) 2311
AvaI CYCGRG 5 cut(s) 110, 1712, 2939, 3383, 5618
AvaII GGWCC 8 cut(s) 181, 556, 1357, 2693, 3856, 4756, 4999, 5521
AxyI CCTNAGG 2 cut(s) 1149, 1218
BaeGI GKGCMC 1 cut(s) 4855
BaeI ACNNNNGTAYC 4 cut(s) 3078, 3111, 5524, 5557
BalI TGGCCA 2 cut(s) 1000, 1518
BamHI GGATCC 3 cut(s) 3598, 6124, 6284
BanI GGYRCC 2 cut(s) 1388, 6056
BauI CACGAG 1 cut(s) 3132
BbsI GAAGAC 3 cut(s) 1992, 3284, 4194
Bbv12I GWGCWC 2 cut(s) 2497, 4728
BceAI ACGGC 4 cut(s) 220, 1879, 4209, 5624
BcgI CGANNNNNNTGC 2 cut(s) 5126, 5160
BciVI GTATCC 5 cut(s) 1794, 2467, 2574, 4487, 5552
BclI TGATCA 4 cut(s) 763, 1066, 5163, 5914
BcnI CCSGG 3 cut(s) 749, 1868, 5761
BcuI ACTAGT 1 cut(s) 3827
BfmI CTRYAG 3 cut(s) 3966, 6061, 6079
BfoI RGCGCY 3 cut(s) 4541, 5173, 6358
BfrI CTTAAG 1 cut(s) 3914
BfuI GTATCC 5 cut(s) 1794, 2467, 2574, 4487, 5552
BglII AGATCT 3 cut(s) 346, 847, 1550
BlpI GCTNAGC 1 cut(s) 3439
BmcAI AGTACT 1 cut(s) 2094
Bme18I GGWCC 8 cut(s) 181, 556, 1357, 2693, 3856, 4756, 4999, 5521
BmeT110I CYCGRG 5 cut(s) 110, 1712, 2939, 3383, 5618
BmgBI CACGTC 1 cut(s) 4811
BmrI ACTGGG 3 cut(s) 168, 1199, 4765
BmtI GCTAGC 1 cut(s) 2315
BmuI ACTGGG 3 cut(s) 168, 1199, 4765
BoxI GACNNNNGTC 1 cut(s) 1313
BpiI GAAGAC 3 cut(s) 1992, 3284, 4194
BplI GAGNNNNNCTC 2 cut(s) 1006, 1038
BpmI CTGGAG 4 cut(s) 2004, 2430, 4163, 4986
Bpu10I CCTNAGC 1 cut(s) 4455
Bpu1102I GCTNAGC 1 cut(s) 3439
Bpu14I TTCGAA 2 cut(s) 4352, 5877
BpuEI CTTGAG 8 cut(s) 313, 516, 2486, 2777, 3450, 3709, 5504, 5726
BpuMI CCSGG 3 cut(s) 749, 1868, 5761
Bsa29I ATCGAT 1 cut(s) 1605
BsaAI YACGTR 2 cut(s) 1739, 2660
BsaBI GATNNNNATC 4 cut(s) 351, 1071, 1140, 3564
BsaHI GRCGYC 1 cut(s) 298
BsaI GGTCTC 2 cut(s) 4888, 6060
BsaWI WCCGGW 2 cut(s) 4758, 5552
BsaXI ACNNNNNCTCC 4 cut(s) 4560, 4590, 5332, 5362
Bse118I RCCGGY 1 cut(s) 5488
Bse21I CCTNAGG 2 cut(s) 1149, 1218
Bse3DI GCAATG 5 cut(s) 629, 1245, 2712, 3339, 6137
Bse8I GATNNNNATC 4 cut(s) 351, 1071, 1140, 3564
BseCI ATCGAT 1 cut(s) 1605
BseJI GATNNNNATC 4 cut(s) 351, 1071, 1140, 3564
BseMI GCAATG 5 cut(s) 629, 1245, 2712, 3339, 6137
BsePI GCGCGC 1 cut(s) 5133
BseRI GAGGAG 4 cut(s) 911, 1061, 2832, 3712
BseSI GKGCMC 1 cut(s) 4855
BseYI CCCAGC 2 cut(s) 2126, 2390
BsgI GTGCAG 3 cut(s) 56, 3066, 5727
Bsh1236I CGCG 8 cut(s) 106, 564, 690, 702, 1775, 2121, 3544, 5135
BshNI GGYRCC 2 cut(s) 1388, 6056
BshVI ATCGAT 1 cut(s) 1605
BsiHKAI GWGCWC 2 cut(s) 2497, 4728
BsiHKCI CYCGRG 5 cut(s) 110, 1712, 2939, 3383, 5618
BsiSI CCGG 6 cut(s) 749, 1868, 4759, 5489, 5553, 5761
BslFI GGGAC 4 cut(s) 1343, 1428, 3155, 3160
BsmBI CGTCTC 1 cut(s) 289
BsmFI GGGAC 4 cut(s) 1343, 1428, 3155, 3160
BsmI GAATGC 5 cut(s) 710, 2434, 2672, 4595, 6386
Bso31I GGTCTC 2 cut(s) 4888, 6060
BsoBI CYCGRG 5 cut(s) 110, 1712, 2939, 3383, 5618
Bsp119I TTCGAA 2 cut(s) 4352, 5877
Bsp1286I GDGCHC 3 cut(s) 2497, 4728, 4855
Bsp1720I GCTNAGC 1 cut(s) 3439
Bsp19I CCATGG 1 cut(s) 2379
Bsp68I TCGCGA 1 cut(s) 2121
BspDI ATCGAT 1 cut(s) 1605
BspFNI CGCG 8 cut(s) 106, 564, 690, 702, 1775, 2121, 3544, 5135
BspHI TCATGA 1 cut(s) 2770
BspMAI CTGCAG 1 cut(s) 6083
BspOI GCTAGC 1 cut(s) 2315
BspQI GCTCTTC 1 cut(s) 214
BspT104I TTCGAA 2 cut(s) 4352, 5877
BspT107I GGYRCC 2 cut(s) 1388, 6056
BspTI CTTAAG 1 cut(s) 3914
BspTNI GGTCTC 2 cut(s) 4888, 6060
BsrBI CCGCTC 4 cut(s) 6, 3873, 4091, 6254
BsrDI GCAATG 5 cut(s) 629, 1245, 2712, 3339, 6137
BsrFI RCCGGY 1 cut(s) 5488
BssAI RCCGGY 1 cut(s) 5488
BssHII GCGCGC 1 cut(s) 5133
BssNAI GTATAC 1 cut(s) 2327
BssNI GRCGYC 1 cut(s) 298
BssSI CACGAG 1 cut(s) 3132
BssT1I CCWWGG 9 cut(s) 94, 1480, 1612, 2241, 2379, 2724, 3652, 3935, 4906
Bst1107I GTATAC 1 cut(s) 2327
Bst2BI CACGAG 1 cut(s) 3132
Bst6I CTCTTC 8 cut(s) 214, 662, 1737, 2724, 2883, 3486, 3775, 4920
BstACI GRCGYC 1 cut(s) 298
BstAFI CTTAAG 1 cut(s) 3914
BstAPI GCANNNNNTGC 3 cut(s) 2915, 6051, 6234
BstBAI YACGTR 2 cut(s) 1739, 2660
BstBI TTCGAA 2 cut(s) 4352, 5877
BstC8I GCNNGC 8 cut(s) 377, 2313, 3875, 4086, 5135, 5139, 5438, 6043
BstDSI CCRYGG 1 cut(s) 2379
BstENI CCTNNNNNAGG 2 cut(s) 2939, 4484
BstFNI CGCG 8 cut(s) 106, 564, 690, 702, 1775, 2121, 3544, 5135
BstH2I RGCGCY 3 cut(s) 4541, 5173, 6358
BstNSI RCATGY 1 cut(s) 1854
BstPAI GACNNNNGTC 1 cut(s) 1313
BstSFI CTRYAG 3 cut(s) 3966, 6061, 6079
BstSLI GKGCMC 1 cut(s) 4855
BstUI CGCG 8 cut(s) 106, 564, 690, 702, 1775, 2121, 3544, 5135
BstV2I GAAGAC 3 cut(s) 1992, 3284, 4194
BstXI CCANNNNNNTGG 2 cut(s) 2386, 4467
BstZ17I GTATAC 1 cut(s) 2327
Bsu15I ATCGAT 1 cut(s) 1605
Bsu36I CCTNAGG 2 cut(s) 1149, 1218
BsuI GTATCC 5 cut(s) 1794, 2467, 2574, 4487, 5552
BsuTUI ATCGAT 1 cut(s) 1605
BtgI CCRYGG 1 cut(s) 2379
BtgZI GCGATG 1 cut(s) 1793
BtrI CACGTC 1 cut(s) 4811
BtsI GCAGTG 4 cut(s) 3054, 4728, 5710, 6275
BtuMI TCGCGA 1 cut(s) 2121
Cac8I GCNNGC 8 cut(s) 377, 2313, 3875, 4086, 5135, 5139, 5438, 6043
CaiI CAGNNNCTG 4 cut(s) 1165, 1600, 4514, 6051
CciI TCATGA 1 cut(s) 2770
Cfr10I RCCGGY 1 cut(s) 5488
ClaI ATCGAT 1 cut(s) 1605
CseI GACGC 4 cut(s) 306, 1589, 3278, 3356
CsiI ACCWGGT 2 cut(s) 958, 5037
CspCI CAANNNNNGTGG 6 cut(s) 1366, 1401, 2230, 2265, 6286, 6321
DraI TTTAAA 1 cut(s) 2530
DraIII CACNNNGTG 1 cut(s) 4859
EaeI YGGCCR 3 cut(s) 998, 1516, 4194
Eam1104I CTCTTC 8 cut(s) 214, 662, 1737, 2724, 2883, 3486, 3775, 4920
EarI CTCTTC 8 cut(s) 214, 662, 1737, 2724, 2883, 3486, 3775, 4920
EciI GGCGGA 2 cut(s) 224, 3497
Eco130I CCWWGG 9 cut(s) 94, 1480, 1612, 2241, 2379, 2724, 3652, 3935, 4906
Eco147I AGGCCT 1 cut(s) 3811
Eco31I GGTCTC 2 cut(s) 4888, 6060
Eco32I GATATC 5 cut(s) 780, 3237, 3616, 4837, 5464
Eco47I GGWCC 8 cut(s) 181, 556, 1357, 2693, 3856, 4756, 4999, 5521
Eco47III AGCGCT 2 cut(s) 4539, 5171
Eco57I CTGAAG 7 cut(s) 1187, 1796, 1970, 2133, 3834, 4722, 6012
Eco81I CCTNAGG 2 cut(s) 1149, 1218
Eco88I CYCGRG 5 cut(s) 110, 1712, 2939, 3383, 5618
EcoNI CCTNNNNNAGG 2 cut(s) 2939, 4484
EcoO109I RGGNCCY 2 cut(s) 1748, 4627
EcoRI GAATTC 2 cut(s) 4276, 5077
EcoRV GATATC 5 cut(s) 780, 3237, 3616, 4837, 5464
EcoT14I CCWWGG 9 cut(s) 94, 1480, 1612, 2241, 2379, 2724, 3652, 3935, 4906
EcoT22I ATGCAT 3 cut(s) 2138, 5836, 6386
ErhI CCWWGG 9 cut(s) 94, 1480, 1612, 2241, 2379, 2724, 3652, 3935, 4906
Esp3I CGTCTC 1 cut(s) 289
FalI AAGNNNNNCTT 8 cut(s) 333, 365, 1473, 1505, 3645, 3677, 5462, 5494
FaqI GGGAC 4 cut(s) 1343, 1428, 3155, 3160
FauI CCCGC 1 cut(s) 3866
FauNDI CATATG 2 cut(s) 942, 1312
FbaI TGATCA 4 cut(s) 763, 1066, 5163, 5914
FblI GTMKAC 1 cut(s) 2326
FspI TGCGCA 1 cut(s) 2171
GsaI CCCAGC 2 cut(s) 2130, 2394
GsuI CTGGAG 4 cut(s) 2004, 2430, 4163, 4986
HaeII RGCGCY 3 cut(s) 4541, 5173, 6358
HapII CCGG 6 cut(s) 749, 1868, 4759, 5489, 5553, 5761
HgaI GACGC 4 cut(s) 306, 1589, 3278, 3356
Hin1I GRCGYC 1 cut(s) 298
HincII GTYRAC 4 cut(s) 3032, 3634, 4501, 4821
HindII GTYRAC 4 cut(s) 3032, 3634, 4501, 4821
HindIII AAGCTT 8 cut(s) 489, 731, 3819, 3960, 4082, 4355, 5261, 5434
HpaI GTTAAC 1 cut(s) 4821
HpaII CCGG 6 cut(s) 749, 1868, 4759, 5489, 5553, 5761
Hpy99I CGWCG 2 cut(s) 1583, 4670
Hsp92I GRCGYC 1 cut(s) 298
Ksp22I TGATCA 4 cut(s) 763, 1066, 5163, 5914
KspAI GTTAAC 1 cut(s) 4821
LguI GCTCTTC 1 cut(s) 214
LmnI GCTCC 8 cut(s) 1985, 2411, 4568, 4731, 4967, 5247, 5598, 5860
MabI ACCWGGT 2 cut(s) 958, 5037
MbiI CCGCTC 4 cut(s) 6, 3873, 4091, 6254
MfeI CAATTG 4 cut(s) 419, 597, 2673, 5159
MhlI GDGCHC 3 cut(s) 2497, 4728, 4855
MlsI TGGCCA 2 cut(s) 1000, 1518
MluNI TGGCCA 2 cut(s) 1000, 1518
MlyI GAGTC 5 cut(s) 539, 1973, 2617, 3685, 6080
MmeI TCCRAC 3 cut(s) 709, 1586, 4734
Mox20I TGGCCA 2 cut(s) 1000, 1518
Mph1103I ATGCAT 3 cut(s) 2138, 5836, 6386
MroXI GAANNNNTTC 3 cut(s) 1663, 2549, 3256
MscI TGGCCA 2 cut(s) 1000, 1518
MslI CAYNNNNRTG 7 cut(s) 1352, 1767, 2288, 2384, 2540, 5447, 5771
Msp20I TGGCCA 2 cut(s) 1000, 1518
MspA1I CMGCKG 3 cut(s) 50, 2912, 6411
MspCI CTTAAG 1 cut(s) 3914
MspI CCGG 6 cut(s) 749, 1868, 4759, 5489, 5553, 5761
MunI CAATTG 4 cut(s) 419, 597, 2673, 5159
Mva1269I GAATGC 5 cut(s) 710, 2434, 2672, 4595, 6386
MvnI CGCG 8 cut(s) 106, 564, 690, 702, 1775, 2121, 3544, 5135
NciI CCSGG 3 cut(s) 749, 1868, 5761
NcoI CCATGG 1 cut(s) 2379
NdeI CATATG 2 cut(s) 942, 1312
NheI GCTAGC 1 cut(s) 2311
NmeAIII GCCGAG 2 cut(s) 2114, 2875
NruI TCGCGA 1 cut(s) 2121
NsbI TGCGCA 1 cut(s) 2171
NsiI ATGCAT 3 cut(s) 2138, 5836, 6386
NspI RCATGY 1 cut(s) 1854
NspV TTCGAA 2 cut(s) 4352, 5877
PacI TTAATTAA 1 cut(s) 2745
PaeR7I CTCGAG 3 cut(s) 1712, 2939, 3383
PagI TCATGA 1 cut(s) 2770
PauI GCGCGC 1 cut(s) 5133
PceI AGGCCT 1 cut(s) 3811
PciSI GCTCTTC 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 523
PctI GAATGC 5 cut(s) 710, 2434, 2672, 4595, 6386
PdmI GAANNNNTTC 3 cut(s) 1663, 2549, 3256
PflMI CCANNNNNTGG 1 cut(s) 4907
PfoI TCCNGGA 3 cut(s) 747, 2268, 6389
PleI GAGTC 5 cut(s) 539, 1972, 2617, 3685, 6080
PpsI GAGTC 5 cut(s) 539, 1972, 2617, 3685, 6080
Ppu21I YACGTR 2 cut(s) 1739, 2660
PshAI GACNNNNGTC 1 cut(s) 1313
PshBI ATTAAT 1 cut(s) 3951
PsiI TTATAA 1 cut(s) 4067
Psp1406I AACGTT 3 cut(s) 3630, 4823, 5922
PspFI CCCAGC 2 cut(s) 2126, 2390
PspXI VCTCGAGB 1 cut(s) 2939
PstI CTGCAG 1 cut(s) 6083
PstNI CAGNNNCTG 4 cut(s) 1165, 1600, 4514, 6051
PteI GCGCGC 1 cut(s) 5133
PvuII CAGCTG 2 cut(s) 2912, 6411
RruI TCGCGA 1 cut(s) 2121
RseI CAYNNNNRTG 7 cut(s) 1352, 1767, 2288, 2384, 2540, 5447, 5771
SapI GCTCTTC 1 cut(s) 214
ScaI AGTACT 1 cut(s) 2094
SchI GAGTC 5 cut(s) 539, 1973, 2617, 3685, 6080
SduI GDGCHC 3 cut(s) 2497, 4728, 4855
SexAI ACCWGGT 2 cut(s) 958, 5037
SfcI CTRYAG 3 cut(s) 3966, 6061, 6079
Sfr274I CTCGAG 3 cut(s) 1712, 2939, 3383
SfuI TTCGAA 2 cut(s) 4352, 5877
SinI GGWCC 8 cut(s) 181, 556, 1357, 2693, 3856, 4756, 4999, 5521
SlaI CTCGAG 3 cut(s) 1712, 2939, 3383
SmiMI CAYNNNNRTG 7 cut(s) 1352, 1767, 2288, 2384, 2540, 5447, 5771
SpeI ACTAGT 1 cut(s) 3827
SseBI AGGCCT 1 cut(s) 3811
SspI AATATT 3 cut(s) 1792, 1940, 4048
StuI AGGCCT 1 cut(s) 3811
StyI CCWWGG 9 cut(s) 94, 1480, 1612, 2241, 2379, 2724, 3652, 3935, 4906
TatI WGTACW 4 cut(s) 225, 2092, 3355, 5964
TauI GCSGC 7 cut(s) 53, 109, 254, 1778, 4091, 5064, 6254
Van91I CCANNNNNTGG 1 cut(s) 4907
Vha464I CTTAAG 1 cut(s) 3914
VpaK11BI GGWCC 8 cut(s) 181, 556, 1357, 2693, 3856, 4756, 4999, 5521
VspI ATTAAT 1 cut(s) 3951
XagI CCTNNNNNAGG 2 cut(s) 2939, 4484
XbaI TCTAGA 1 cut(s) 5081
XceI RCATGY 1 cut(s) 1854
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XhoI CTCGAG 3 cut(s) 1712, 2939, 3383
XmiI GTMKAC 1 cut(s) 2326
XmnI GAANNNNTTC 3 cut(s) 1663, 2549, 3256
ZrmI AGTACT 1 cut(s) 2094
Zsp2I ATGCAT 3 cut(s) 2138, 5836, 6386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.