Rroxscaffold_5G00333720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0019564
Physical Location & Seq
Reverse (-)
1099232 .. 1099855
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00333720.1

Sequence Viewer

Length: 351 bp
ATGTGGGACCGACATGTCTCGGCTCCCGCTCAGGAGCTTCGATGCTATCTTGGCGTGGCGCGTGGCGGCGCGTCGACGGCGATCCGGGGATGGTCCGACGCCGAGGTTGTAGCCCGCCCGGCGGTGTGTCCGCATGTCATTCCTTTAGTTGGAATTAGTACCTTTGGCGGTGAGGAAGAGGCTGATCTTCTTCTCTTTGTTTTCGATGTGGGACCGACATGTCTCGGTTCCCGCTCGGAGCTTCGATGCTATCTTGGCGTGGCGCGTGGCGGCGCGTCGACGGCGATCCGGGGATGGTCCGACGCCGAGGTTGTAGCCCGCCCGGCGTGTGTCCGCATGTCATTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

12.32

Weight (kDa)

6.07

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 14, 219
AccBSI CCGCTC 2 cut(s) 29, 234
AccI GTMKAC 2 cut(s) 74, 278
AccII CGCG 4 cut(s) 61, 71, 265, 275
AclWI GGATC 2 cut(s) 76, 280
AcyI GRCGYC 2 cut(s) 99, 303
AfaI GTAC 1 cut(s) 160
AfiI CCNNNNNNNGG 2 cut(s) 121, 149
AflIII ACRYGT 2 cut(s) 13, 218
AluBI AGCT 2 cut(s) 37, 241
AluI AGCT 2 cut(s) 37, 241
Alw26I GTCTC 2 cut(s) 22, 227
AlwI GGATC 2 cut(s) 76, 280
AspLEI GCGC 4 cut(s) 61, 71, 265, 275
AspS9I GGNCC 4 cut(s) 7, 93, 212, 297
AsuC2I CCSGG 4 cut(s) 86, 119, 290, 323
AsuHPI GGTGA 1 cut(s) 182
AvaII GGWCC 4 cut(s) 7, 93, 212, 297
BccI CCATC 2 cut(s) 84, 288
BceAI ACGGC 2 cut(s) 93, 297
BcnI CCSGG 4 cut(s) 86, 119, 290, 323
BcoDI GTCTC 2 cut(s) 22, 227
BglI GCCNNNNNGGC 2 cut(s) 119, 323
BisI GCNGC 2 cut(s) 67, 271
BlsI GCNGC 2 cut(s) 68, 272
Bme1390I CCNGG 4 cut(s) 86, 119, 290, 323
Bme18I GGWCC 4 cut(s) 7, 93, 212, 297
BmgT120I GGNCC 4 cut(s) 7, 93, 212, 297
BmiI GGNNCC 4 cut(s) 8, 24, 213, 229
BmrFI CCNGG 4 cut(s) 86, 119, 290, 323
BmsI GCATC 2 cut(s) 32, 236
Bpu10I CCTNAGC 1 cut(s) 30
BpuMI CCSGG 4 cut(s) 86, 119, 290, 323
BsaHI GRCGYC 2 cut(s) 99, 303
BsaJI CCNNGG 4 cut(s) 85, 102, 289, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 149
BseDI CCNNGG 4 cut(s) 85, 102, 289, 306
BseGI GGATG 2 cut(s) 95, 299
BseLI CCNNNNNNNGG 2 cut(s) 121, 149
BseMII CTCAG 1 cut(s) 44
Bsh1236I CGCG 4 cut(s) 61, 71, 265, 275
BsiSI CCGG 4 cut(s) 85, 119, 289, 323
BslFI GGGAC 2 cut(s) 20, 225
BslI CCNNNNNNNGG 2 cut(s) 121, 149
BsmAI GTCTC 2 cut(s) 22, 227
BsmFI GGGAC 2 cut(s) 20, 225
Bsp143I GATC 3 cut(s) 81, 184, 285
BspCNI CTCAG 1 cut(s) 43
BspFNI CGCG 4 cut(s) 61, 71, 265, 275
BspLI GGNNCC 4 cut(s) 8, 24, 213, 229
BspPI GGATC 2 cut(s) 76, 280
BsrBI CCGCTC 2 cut(s) 29, 234
BssECI CCNNGG 4 cut(s) 85, 102, 289, 306
BssMI GATC 3 cut(s) 81, 184, 285
BssNI GRCGYC 2 cut(s) 99, 303
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 2 cut(s) 99, 303
BstC8I GCNNGC 2 cut(s) 115, 319
BstDEI CTNAG 1 cut(s) 30
BstF5I GGATG 2 cut(s) 95, 299
BstFNI CGCG 4 cut(s) 61, 71, 265, 275
BstHHI GCGC 4 cut(s) 61, 71, 265, 275
BstKTI GATC 3 cut(s) 84, 187, 288
BstMAI GTCTC 2 cut(s) 22, 227
BstMBI GATC 3 cut(s) 81, 184, 285
BstMWI GCNNNNNNNGC 6 cut(s) 51, 77, 119, 255, 281, 323
BstNSI RCATGY 4 cut(s) 17, 137, 222, 340
BstSCI CCNGG 4 cut(s) 84, 117, 288, 321
BstUI CGCG 4 cut(s) 61, 71, 265, 275
BtsCI GGATG 2 cut(s) 95, 299
Cac8I GCNNGC 2 cut(s) 115, 319
CfoI GCGC 4 cut(s) 61, 71, 265, 275
Cfr13I GGNCC 4 cut(s) 7, 93, 212, 297
CseI GACGC 4 cut(s) 60, 107, 264, 311
Csp6I GTAC 1 cut(s) 159
CviAII CATG 4 cut(s) 14, 134, 219, 337
CviJI RGCY 6 cut(s) 23, 37, 113, 182, 241, 317
CviKI_1 RGCY 6 cut(s) 23, 37, 113, 182, 241, 317
CviQI GTAC 1 cut(s) 159
DdeI CTNAG 1 cut(s) 30
DpnI GATC 3 cut(s) 83, 186, 287
DpnII GATC 3 cut(s) 81, 184, 285
DrdI GACNNNNNNGTC 2 cut(s) 14, 219
DseDI GACNNNNNNGTC 2 cut(s) 14, 219
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco47I GGWCC 4 cut(s) 7, 93, 212, 297
FaeI CATG 4 cut(s) 17, 137, 222, 340
FaiI YATR 4 cut(s) 15, 135, 220, 338
FaqI GGGAC 2 cut(s) 20, 225
FatI CATG 4 cut(s) 13, 133, 218, 336
FauI CCCGC 4 cut(s) 34, 122, 239, 326
FblI GTMKAC 2 cut(s) 74, 278
Fnu4HI GCNGC 2 cut(s) 67, 271
FokI GGATG 2 cut(s) 102, 306
Fsp4HI GCNGC 2 cut(s) 67, 271
GlaI GCGC 4 cut(s) 60, 70, 264, 274
GluI GCNGC 2 cut(s) 67, 271
HapII CCGG 4 cut(s) 85, 119, 289, 323
HgaI GACGC 4 cut(s) 60, 107, 264, 311
HhaI GCGC 4 cut(s) 61, 71, 265, 275
Hin1I GRCGYC 2 cut(s) 99, 303
Hin1II CATG 4 cut(s) 17, 137, 222, 340
Hin6I GCGC 4 cut(s) 59, 69, 263, 273
HinP1I GCGC 4 cut(s) 59, 69, 263, 273
HincII GTYRAC 2 cut(s) 75, 279
HindII GTYRAC 2 cut(s) 75, 279
HpaII CCGG 4 cut(s) 85, 119, 289, 323
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 2 cut(s) 75, 279
Hpy188I TCNGA 3 cut(s) 97, 238, 301
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 2 cut(s) 75, 279
Hpy99I CGWCG 6 cut(s) 76, 79, 101, 280, 283, 305
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 77, 119, 255, 281, 323
HpyF3I CTNAG 1 cut(s) 30
Hsp92I GRCGYC 2 cut(s) 99, 303
Hsp92II CATG 4 cut(s) 17, 137, 222, 340
HspAI GCGC 4 cut(s) 59, 69, 263, 273
Kzo9I GATC 3 cut(s) 81, 184, 285
LmnI GCTCC 3 cut(s) 28, 34, 238
LpnPI CCDG 5 cut(s) 17, 98, 132, 302, 336
LweI GCATC 2 cut(s) 32, 236
MalI GATC 3 cut(s) 83, 186, 287
MbiI CCGCTC 2 cut(s) 29, 234
MboI GATC 3 cut(s) 81, 184, 285
MboII GAAGA 3 cut(s) 179, 182, 188
MluCI AATT 1 cut(s) 153
MmeI TCCRAC 3 cut(s) 120, 130, 324
MnlI CCTC 4 cut(s) 97, 166, 172, 301
MspI CCGG 4 cut(s) 85, 119, 289, 323
MspR9I CCNGG 4 cut(s) 86, 119, 290, 323
MvnI CGCG 4 cut(s) 61, 71, 265, 275
MwoI GCNNNNNNNGC 6 cut(s) 51, 77, 119, 255, 281, 323
NciI CCSGG 4 cut(s) 86, 119, 290, 323
NdeII GATC 3 cut(s) 81, 184, 285
NlaIII CATG 4 cut(s) 17, 137, 222, 340
NlaIV GGNNCC 4 cut(s) 8, 24, 213, 229
NmeAIII GCCGAG 2 cut(s) 127, 331
NspI RCATGY 4 cut(s) 17, 137, 222, 340
PciI ACATGT 2 cut(s) 13, 218
PkrI GCNGC 2 cut(s) 68, 272
PscI ACATGT 2 cut(s) 13, 218
PspN4I GGNNCC 4 cut(s) 8, 24, 213, 229
PspPI GGNCC 4 cut(s) 7, 93, 212, 297
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
SalI GTCGAC 2 cut(s) 73, 277
SatI GCNGC 2 cut(s) 67, 271
Sau3AI GATC 3 cut(s) 81, 184, 285
Sau96I GGNCC 4 cut(s) 7, 93, 212, 297
ScrFI CCNGG 4 cut(s) 86, 119, 290, 323
SetI ASST 5 cut(s) 39, 108, 164, 243, 312
SfaNI GCATC 2 cut(s) 32, 236
SgrDI CGTCGACG 2 cut(s) 73, 277
SinI GGWCC 4 cut(s) 7, 93, 212, 297
Sse9I AATT 1 cut(s) 153
StyD4I CCNGG 4 cut(s) 84, 117, 288, 321
TaqI TCGA 5 cut(s) 40, 74, 204, 244, 278
TaqII GACCGA 2 cut(s) 24, 229
TasI AATT 1 cut(s) 153
TauI GCSGC 2 cut(s) 69, 273
VpaK11BI GGWCC 4 cut(s) 7, 93, 212, 297
XceI RCATGY 4 cut(s) 17, 137, 222, 340
XmiI GTMKAC 2 cut(s) 74, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.