Rroxscaffold_5G00380310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0019564
Physical Location & Seq
Forward (+)
60463345 .. 60482991
19647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380310.1

Sequence Viewer

Length: 399 bp
ATGACGACGACGACGATAATGATTCGTTACTGCTTGAGTACGTTCTGGGCAGGTGGTGAAATAAATTTCACGTGTATGCATGGCTCTCTCTCCTTTCCACTTCCACAGAGCAACTTCAATTCCTCCGGCCAACTAGCAGCGACTCTTAAACGAACCCATGGGAGATTGCAAGAAACATCATCTCGATCCACAAGGTTAAGAAAGATCGAAGTGGGCTCTTCCATGTTTTCAATGTGGGACCGATATGCTTCGGTTGCAGCTTCGGAGGTTCGATGCCATCTTGGAGCGGCGCGTGGCGGCTCATCAGCGGTGATCCGAGGGTTGGAGACACAACTTCCAAAAGGTCGGTCCGCAATCTCACACCTGCATTTTGGGTTTTTGGATATCAGTGTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.43

Weight (kDa)

10.18

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 41, 372
Acc36I ACCTGC 2 cut(s) 41, 372
AccBSI CCGCTC 1 cut(s) 287
AccII CGCG 1 cut(s) 292
AciI CCGC 4 cut(s) 287, 297, 308, 351
AclWI GGATC 2 cut(s) 180, 307
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 64
AcvI CACGTG 1 cut(s) 72
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 1 cut(s) 322
AflIII ACRYGT 1 cut(s) 71
AgsI TTSAA 2 cut(s) 118, 231
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw26I GTCTC 1 cut(s) 320
AlwI GGATC 2 cut(s) 180, 307
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 2 cut(s) 137, 257
ApoI RAATTY 1 cut(s) 64
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 2 cut(s) 238, 348
AsuHPI GGTGA 2 cut(s) 68, 322
AvaII GGWCC 2 cut(s) 238, 348
BanII GRGCYC 1 cut(s) 218
BbrPI CACGTG 1 cut(s) 72
BbvI GCAGC 2 cut(s) 149, 269
BccI CCATC 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 320
BfaI CTAG 1 cut(s) 134
BfuAI ACCTGC 2 cut(s) 41, 372
BisI GCNGC 4 cut(s) 138, 258, 288, 298
BlsI GCNGC 4 cut(s) 139, 259, 289, 299
Bme18I GGWCC 2 cut(s) 238, 348
BmgT120I GGNCC 2 cut(s) 238, 348
BmiI GGNNCC 1 cut(s) 239
BmsI GCATC 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 55
BsaAI YACGTR 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 157, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 322
BseDI CCNNGG 2 cut(s) 157, 316
BseLI CCNNNNNNNGG 1 cut(s) 322
BseXI GCAGC 2 cut(s) 149, 269
Bsh1236I CGCG 1 cut(s) 292
BshFI GGCC 1 cut(s) 129
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 1 cut(s) 320
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 218
Bsp143I GATC 3 cut(s) 185, 204, 312
Bsp19I CCATGG 1 cut(s) 157
BspACI CCGC 4 cut(s) 287, 297, 308, 351
BspANI GGCC 1 cut(s) 129
BspFNI CGCG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 239
BspMI ACCTGC 2 cut(s) 41, 372
BspPI GGATC 2 cut(s) 180, 307
BspQI GCTCTTC 1 cut(s) 223
BsrBI CCGCTC 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 157, 316
BssMI GATC 3 cut(s) 185, 204, 312
BssT1I CCWWGG 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 223
BstBAI YACGTR 1 cut(s) 72
BstDSI CCRYGG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 292
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 3 cut(s) 188, 207, 315
BstMAI GTCTC 1 cut(s) 320
BstMBI GATC 3 cut(s) 185, 204, 312
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstUI CGCG 1 cut(s) 292
BstV1I GCAGC 2 cut(s) 149, 269
BsuRI GGCC 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 394
BveI ACCTGC 2 cut(s) 41, 372
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 238, 348
CpoI CGGWCCG 1 cut(s) 348
Csp6I GTAC 1 cut(s) 39
CspI CGGWCCG 1 cut(s) 348
CviAII CATG 3 cut(s) 80, 158, 223
CviJI RGCY 5 cut(s) 84, 129, 216, 260, 300
CviKI_1 RGCY 5 cut(s) 84, 129, 216, 260, 300
CviQI GTAC 1 cut(s) 39
DpnI GATC 3 cut(s) 187, 206, 314
DpnII GATC 3 cut(s) 185, 204, 312
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 218
Eco32I GATATC 1 cut(s) 385
Eco47I GGWCC 2 cut(s) 238, 348
Eco72I CACGTG 1 cut(s) 72
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 157
EcoT22I ATGCAT 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 3 cut(s) 83, 161, 226
FaiI YATR 5 cut(s) 77, 81, 159, 224, 246
FaqI GGGAC 1 cut(s) 251
FatI CATG 3 cut(s) 79, 157, 222
Fnu4HI GCNGC 4 cut(s) 138, 258, 288, 298
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 4 cut(s) 138, 258, 288, 298
FspBI CTAG 1 cut(s) 134
GlaI GCGC 1 cut(s) 291
GluI GCNGC 4 cut(s) 138, 258, 288, 298
HaeIII GGCC 1 cut(s) 129
HapII CCGG 1 cut(s) 126
HhaI GCGC 1 cut(s) 292
Hin1II CATG 3 cut(s) 83, 161, 226
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HinfI GANTC 2 cut(s) 22, 142
HpaII CCGG 1 cut(s) 126
HphI GGTGA 2 cut(s) 68, 322
Hpy188I TCNGA 2 cut(s) 265, 317
Hpy188III TCNNGA 1 cut(s) 183
Hpy99I CGWCG 3 cut(s) 10, 13, 16
HpyCH4IV ACGT 2 cut(s) 41, 71
HpyCH4V TGCA 4 cut(s) 79, 169, 257, 367
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpySE526I ACGT 2 cut(s) 41, 71
Hsp92II CATG 3 cut(s) 83, 161, 226
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 3 cut(s) 185, 204, 312
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 4 cut(s) 31, 36, 139, 377
Lsp1109I GCAGC 2 cut(s) 149, 269
LweI GCATC 1 cut(s) 263
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 41, 71
MaeIII GTNAC 1 cut(s) 26
MalI GATC 3 cut(s) 187, 206, 314
MbiI CCGCTC 1 cut(s) 287
MboI GATC 3 cut(s) 185, 204, 312
MboII GAAGA 1 cut(s) 210
MhlI GDGCHC 1 cut(s) 218
MluCI AATT 2 cut(s) 64, 118
MlyI GAGTC 1 cut(s) 136
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 3 cut(s) 133, 259, 311
Mph1103I ATGCAT 1 cut(s) 81
MseI TTAA 2 cut(s) 147, 197
MslI CAYNNNNRTG 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 1 cut(s) 126
MvnI CGCG 1 cut(s) 292
MwoI GCNNNNNNNGC 1 cut(s) 254
NcoI CCATGG 1 cut(s) 157
NdeII GATC 3 cut(s) 185, 204, 312
NlaIII CATG 3 cut(s) 83, 161, 226
NlaIV GGNNCC 1 cut(s) 239
NsiI ATGCAT 1 cut(s) 81
PaqCI CACCTGC 2 cut(s) 41, 372
PciSI GCTCTTC 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 4 cut(s) 139, 259, 289, 299
PleI GAGTC 1 cut(s) 136
PmaCI CACGTG 1 cut(s) 72
PmlI CACGTG 1 cut(s) 72
PpsI GAGTC 1 cut(s) 136
Ppu21I YACGTR 1 cut(s) 72
PspCI CACGTG 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 239
PspPI GGNCC 2 cut(s) 238, 348
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
RseI CAYNNNNRTG 1 cut(s) 74
Rsr2I CGGWCCG 1 cut(s) 348
RsrII CGGWCCG 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 2 cut(s) 147, 197
SatI GCNGC 4 cut(s) 138, 258, 288, 298
Sau3AI GATC 3 cut(s) 185, 204, 312
Sau96I GGNCC 2 cut(s) 238, 348
SchI GAGTC 1 cut(s) 136
SduI GDGCHC 1 cut(s) 218
SetI ASST 8 cut(s) 44, 55, 74, 197, 262, 270, 346, 366
SfaNI GCATC 1 cut(s) 263
SinI GGWCC 2 cut(s) 238, 348
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 2 cut(s) 64, 118
SsiI CCGC 4 cut(s) 287, 297, 308, 351
SspMI CTAG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 157
TaiI ACGT 2 cut(s) 44, 74
TaqI TCGA 3 cut(s) 184, 207, 271
TaqII GACCGA 2 cut(s) 255, 336
TasI AATT 2 cut(s) 64, 118
TauI GCSGC 2 cut(s) 290, 300
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 2 cut(s) 147, 197
Tru9I TTAA 2 cut(s) 147, 197
TscAI CASTG 1 cut(s) 394
TseI GCWGC 2 cut(s) 137, 257
TspRI CASTG 1 cut(s) 394
VpaK11BI GGWCC 2 cut(s) 238, 348
XapI RAATTY 1 cut(s) 64
XspI CTAG 1 cut(s) 134
Zsp2I ATGCAT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.