AT2G28470

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006465
Physical Location & Seq
Reverse (-)
12168840 .. 12173991
5152 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28470.1

Sequence Viewer

Length: 2559 bp
ATGGAAATTGCAGCGAAAATGGTTAAAGTAAGGAAGATGGAGATGATTTTATTACTAATTCTTGTGATTGTGGTGGCGGCGACGGCGGCGAATGTGACTTATGACCACCGTGCATTAGTAATCGACGGGAAACGGAAAGTTCTAATCTCTGGTTCTATTCATTATCCTCGGAGTACTCCTGAGATGTGGCCAGAGCTTATACAGAAATCTAAAGACGGTGGTTTAGATGTTATAGAGACGTATGTGTTTTGGAGTGGTCACGAACCGGAGAAAAATAAGTATAATTTTGAAGGAAGATATGATTTAGTGAAATTTGTGAAGCTTGCGGCTAAAGCTGGTCTCTATGTTCATTTAAGAATTGGTCCTTACGTCTGTGCTGAATGGAATTACGGTGGTTTCCCAGTGTGGTTGCATTTTGTTCCAGGAATTAAGTTTCGAACTGATAATGAGCCATTTAAGGAAGAAATGCAGAGATTTACCACAAAGATTGTTGATTTGATGAAGCAAGAAAAGCTTTATGCATCACAAGGAGGTCCAATCATTCTCTCGCAGATTGAGAATGAATATGGAAATATTGACTCAGCTTATGGTGCGGCTGCTAAAAGTTATATCAAGTGGTCTGCTTCTATGGCTCTTTCGTTAGATACTGGAGTACCATGGAATATGTGTCAACAAACAGATGCTCCTGATCCCATGATCAACACATGCAATGGTTTCTACTGTGACCAGTTTACACCTAACTCAAATAATAAACCAAAGATGTGGACCGAGAACTGGAGTGGATGGTTCCTTGGTTTTGGAGATCCTTCTCCTTACAGACCAGTTGAAGATCTTGCATTTGCGGTCGCGCGGTTTTACCAACGAGGTGGAACGTTCCAGAACTATTACATGTATCACGGTGGAACAAACTTTGATAGAACAAGTGGAGGACCATTAATCTCTACTAGTTATGATTATGATGCTCCAATTGATGAGTATGGACTACTTAGACAACCAAAATGGGGACACTTACGAGATCTACACAAGGCTATCAAGCTTTGTGAAGATGCATTGATTGCCACAGATCCAACAATTACTTCTCTAGGTTCAAATTTGGAGGCTGCTGTATATAAAACAGAATCTGGATCATGTGCTGCTTTTCTTGCAAATGTTGACACGAAGTCTGATGCAACTGTGACTTTCAATGGAAAATCATATAACTTGCCTGCATGGTCCGTAAGCATCTTGCCGGATTGCAAAAATGTAGCTTTCAATACCGCAAAGATAAATTCTGCAACTGAGTCTACCGCATTTGCTCGTCAATCATTAAAGCCCGATGGTGGTTCGTCTGCGGAGTTAGGTTCACAATGGAGTTACATTAAAGAACCTATTGGAATTTCCAAAGCTGATGCATTCTTGAAACCTGGATTGCTAGAGCAGATTAACACAACAGCTGATAAAAGCGATTACTTGTGGTACTCACTAAGGACGGATATAAAAGGCGATGAGACTTTCCTTGACGAGGGATCTAAAGCCGTCCTTCACATTGAATCTCTTGGTCAAGTGGTCTATGCTTTTATAAATGGAAAACTTGCAGGAAGCGGACATGGCAAACAGAAGATTTCTTTGGATATACCGATTAATCTTGTAACCGGGACGAACACAATCGATCTCCTTAGTGTTACCGTAGGGCTTGCGAATTATGGAGCTTTCTTTGACTTAGTGGGAGCAGGAATAACCGGACCTGTGACACTTAAAAGCGCTAAAGGTGGTAGCTCAATTGATTTGGCTTCACAGCAATGGACTTATCAGGTTGGACTCAAAGGAGAAGACACAGGTTTGGCAACTGTAGATTCTTCTGAATGGGTTTCAAAGTCTCCTTTGCCTACTAAACAACCACTTATTTGGTACAAGACGACATTTGATGCTCCTTCTGGGAGCGAGCCAGTAGCTATAGACTTCACGGGTACAGGAAAGGGTATTGCATGGGTGAATGGACAGAGCATAGGTAGGTACTGGCCAACTAGTATCGCTGGAAATGGCGGTTGTACAGAATCATGCGACTATAGAGGTTCTTACCGTGCAAACAAATGCCTCAAGAACTGTGGAAAACCTTCACAGACATTGTATCATGTACCTCGCTCGTGGCTAAAACCGAGCGGGAACATACTTGTTCTGTTTGAGGAGATGGGAGGAGATCCAACACAAATATCATTTGCGACAAAACAAACAGGAAGCAATCTTTGTCTAACGGTGTCACAGTCTCATCCACCACCGGTGGACACATGGACTTCCGACTCAAAGATCTCAAACAGAAACAGAACCAGGCCGGTTCTTTCGTTGAAATGCCCTATCTCTACTCAGGTGATATTTTCTATAAAATTTGCAAGCTTTGGTACACCCAAAGGTACTTGCGGTAGCTTCACACAAGGCCATTGCAATAGCTCTCGATCTCTCTCCCTCGTCCAAAAGGCATGTATTGGATTGAGGAGTTGCAACGTTGAAGTATCGACTAGAGTGTTCGGGGAACCTTGTCGTGGCGTCGTCAAGAGCTTAGCTGTTGAAGCTTCTTGTTCATGA

Protein Analysis

852

Amino Acids

93.21

Weight (kDa)

8.09

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 39 - 343 6.3e-123 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 60 - 206 5e-07 Beta-galactosidase
GHD PF17834 366 - 422 1.4e-26 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 627 - 715 3.2e-25 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 774 - 851 1.3e-19 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1559
AccBSI CCGCTC 1 cut(s) 2139
AccI GTMKAC 1 cut(s) 1283
AccII CGCG 2 cut(s) 848, 850
AclI AACGTT 2 cut(s) 872, 2478
AclWI GGATC 6 cut(s) 683, 797, 1058, 1132, 1513, 2171
AcoI YGGCCR 2 cut(s) 188, 1997
AcsI RAATTY 5 cut(s) 311, 1090, 1267, 1374, 2360
AcyI GRCGYC 1 cut(s) 2520
AfeI AGCGCT 1 cut(s) 1741
AfiI CCNNNNNNNGG 5 cut(s) 774, 1001, 1319, 1501, 2516
AflIII ACRYGT 1 cut(s) 888
AgeI ACCGGT 1 cut(s) 2254
AhdI GACNNNNNGTC 1 cut(s) 1159
AhlI ACTAGT 2 cut(s) 944, 2003
AjnI CCWGG 3 cut(s) 421, 1402, 2303
AjuI GAANNNNNNNTTGG 4 cut(s) 1060, 1092, 1589, 1621
Alw26I GTCTC 5 cut(s) 230, 344, 1481, 1860, 2247
AlwI GGATC 6 cut(s) 683, 797, 1058, 1132, 1513, 2171
AlwNI CAGNNNCTG 1 cut(s) 1121
Aor51HI AGCGCT 1 cut(s) 1741
AoxI GGCC 4 cut(s) 188, 1997, 2306, 2410
ApeKI GCWGC 4 cut(s) 11, 596, 1100, 1133
ApoI RAATTY 5 cut(s) 311, 1090, 1267, 1374, 2360
AseI ATTAAT 2 cut(s) 935, 1620
AsiGI ACCGGT 1 cut(s) 2254
Asp700I GAANNNNTTC 1 cut(s) 2092
AspLEI GCGC 2 cut(s) 850, 1742
AspS9I GGNCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
AsuC2I CCSGG 1 cut(s) 1633
AsuHPI GGTGA 2 cut(s) 1981, 2356
AsuII TTCGAA 1 cut(s) 436
AvaII GGWCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
BaeI ACNNNNGTAYC 4 cut(s) 636, 636, 669, 669
BalI TGGCCA 2 cut(s) 190, 1999
BauI CACGAG 1 cut(s) 2122
BbsI GAAGAC 1 cut(s) 1815
BbvI GCAGC 4 cut(s) 23, 583, 1087, 1120
BccI CCATC 4 cut(s) 31, 777, 1310, 2161
BceAI ACGGC 2 cut(s) 99, 1499
BciT130I CCWGG 3 cut(s) 423, 1404, 2305
BclI TGATCA 1 cut(s) 696
BcnI CCSGG 1 cut(s) 1633
BcoDI GTCTC 5 cut(s) 230, 344, 1481, 1860, 2247
BcuI ACTAGT 2 cut(s) 944, 2003
BfaI CTAG 5 cut(s) 945, 1082, 1412, 2004, 2493
BfmI CTRYAG 3 cut(s) 1827, 1932, 2044
BfoI RGCGCY 1 cut(s) 1743
BglII AGATCT 3 cut(s) 829, 1015, 2283
BisI GCNGC 8 cut(s) 12, 78, 87, 327, 594, 597, 1101, 1134
BlpI GCTNAGC 1 cut(s) 2533
BlsI GCNGC 8 cut(s) 13, 79, 88, 328, 595, 598, 1102, 1135
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 4 cut(s) 423, 1404, 1633, 2305
Bme18I GGWCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
BmeRI GACNNNNNGTC 1 cut(s) 1159
BmgT120I GGNCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
BmiI GGNNCC 2 cut(s) 788, 2508
BmrFI CCNGG 4 cut(s) 423, 1404, 1633, 2305
BmrI ACTGGG 1 cut(s) 395
BmsI GCATC 8 cut(s) 530, 670, 949, 1036, 1156, 1230, 1378, 1894
BmuI ACTGGG 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 1815
BpmI CTGGAG 2 cut(s) 669, 796
Bpu1102I GCTNAGC 1 cut(s) 2533
Bpu14I TTCGAA 1 cut(s) 436
BpuEI CTTGAG 1 cut(s) 2060
BpuMI CCSGG 1 cut(s) 1633
Bsa29I ATCGAT 1 cut(s) 1647
BsaHI GRCGYC 1 cut(s) 2520
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 167, 656, 790
BsaWI WCCGGW 3 cut(s) 265, 1718, 2254
BsaXI ACNNNNNCTCC 2 cut(s) 667, 697
Bsc4I CCNNNNNNNGG 5 cut(s) 774, 1001, 1319, 1501, 2516
Bse118I RCCGGY 2 cut(s) 2254, 2308
Bse1I ACTGG 7 cut(s) 401, 652, 727, 779, 821, 1925, 2000
Bse3DI GCAATG 3 cut(s) 715, 1784, 2413
BseBI CCWGG 3 cut(s) 423, 1404, 2305
BseCI ATCGAT 1 cut(s) 1647
BseDI CCNNGG 3 cut(s) 167, 656, 790
BseGI GGATG 2 cut(s) 788, 2245
BseLI CCNNNNNNNGG 5 cut(s) 774, 1001, 1319, 1501, 2516
BseMI GCAATG 3 cut(s) 715, 1784, 2413
BseMII CTCAG 4 cut(s) 171, 594, 1269, 2354
BseNI ACTGG 7 cut(s) 401, 652, 727, 779, 821, 1925, 2000
BseRI GAGGAG 3 cut(s) 2177, 2187, 2482
BseXI GCAGC 4 cut(s) 23, 583, 1087, 1120
Bsh1236I CGCG 2 cut(s) 848, 850
Bsh1285I CGRYCG 1 cut(s) 846
BshFI GGCC 4 cut(s) 190, 1999, 2308, 2412
BshTI ACCGGT 1 cut(s) 2254
BshVI ATCGAT 1 cut(s) 1647
BsiEI CGRYCG 1 cut(s) 846
BsiSI CCGG 6 cut(s) 266, 1229, 1632, 1719, 2255, 2309
BslFI GGGAC 2 cut(s) 1017, 1648
BslI CCNNNNNNNGG 5 cut(s) 774, 1001, 1319, 1501, 2516
BsmAI GTCTC 5 cut(s) 230, 344, 1481, 1860, 2247
BsmBI CGTCTC 1 cut(s) 230
BsmFI GGGAC 2 cut(s) 1017, 1648
BsmI GAATGC 1 cut(s) 1391
BsnI GGCC 4 cut(s) 190, 1999, 2308, 2412
Bso31I GGTCTC 1 cut(s) 344
Bsp119I TTCGAA 1 cut(s) 436
Bsp1407I TGTACA 1 cut(s) 2027
Bsp1720I GCTNAGC 1 cut(s) 2533
Bsp19I CCATGG 1 cut(s) 656
BspANI GGCC 4 cut(s) 190, 1999, 2308, 2412
BspCNI CTCAG 4 cut(s) 172, 593, 1270, 2353
BspDI ATCGAT 1 cut(s) 1647
BspFNI CGCG 2 cut(s) 848, 850
BspHI TCATGA 1 cut(s) 2555
BspLI GGNNCC 2 cut(s) 788, 2508
BspPI GGATC 6 cut(s) 683, 797, 1058, 1132, 1513, 2171
BspT104I TTCGAA 1 cut(s) 436
BspTNI GGTCTC 1 cut(s) 344
BsrBI CCGCTC 1 cut(s) 2139
BsrDI GCAATG 3 cut(s) 715, 1784, 2413
BsrFI RCCGGY 2 cut(s) 2254, 2308
BsrGI TGTACA 1 cut(s) 2027
BsrI ACTGG 7 cut(s) 401, 652, 727, 779, 821, 1925, 2000
BssAI RCCGGY 2 cut(s) 2254, 2308
BssECI CCNNGG 3 cut(s) 167, 656, 790
BssNI GRCGYC 1 cut(s) 2520
BssSI CACGAG 1 cut(s) 2122
BssT1I CCWWGG 2 cut(s) 656, 790
Bst2BI CACGAG 1 cut(s) 2122
Bst2UI CCWGG 3 cut(s) 423, 1404, 2305
BstACI GRCGYC 1 cut(s) 2520
BstAPI GCANNNNNTGC 1 cut(s) 1055
BstAUI TGTACA 1 cut(s) 2027
BstBI TTCGAA 1 cut(s) 436
BstC8I GCNNGC 5 cut(s) 324, 1206, 1674, 1922, 2368
BstDEI CTNAG 9 cut(s) 180, 580, 986, 1278, 1463, 1655, 1699, 2340, 2533
BstDSI CCRYGG 1 cut(s) 656
BstENI CCTNNNNNAGG 1 cut(s) 1499
BstF5I GGATG 2 cut(s) 788, 2245
BstFNI CGCG 2 cut(s) 848, 850
BstH2I RGCGCY 1 cut(s) 1743
BstHHI GCGC 2 cut(s) 850, 1742
BstMAI GTCTC 5 cut(s) 230, 344, 1481, 1860, 2247
BstMCI CGRYCG 1 cut(s) 846
BstNI CCWGG 3 cut(s) 423, 1404, 2305
BstNSI RCATGY 3 cut(s) 708, 892, 2457
BstSCI CCNGG 4 cut(s) 421, 1402, 1631, 2303
BstSFI CTRYAG 3 cut(s) 1827, 1932, 2044
BstUI CGCG 2 cut(s) 848, 850
BstV1I GCAGC 4 cut(s) 23, 583, 1087, 1120
BstV2I GAAGAC 1 cut(s) 1815
BstX2I RGATCY 7 cut(s) 802, 829, 1015, 1063, 1505, 2176, 2283
BstXI CCANNNNNNTGG 3 cut(s) 762, 866, 1884
BstYI RGATCY 7 cut(s) 802, 829, 1015, 1063, 1505, 2176, 2283
Bsu15I ATCGAT 1 cut(s) 1647
BsuRI GGCC 4 cut(s) 190, 1999, 2308, 2412
BsuTUI ATCGAT 1 cut(s) 1647
BtgI CCRYGG 1 cut(s) 656
BtgZI GCGATG 1 cut(s) 1497
BtsCI GGATG 2 cut(s) 788, 2245
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 5 cut(s) 324, 1206, 1674, 1922, 2368
CaiI CAGNNNCTG 1 cut(s) 1121
CciI TCATGA 1 cut(s) 2555
CfoI GCGC 2 cut(s) 850, 1742
Cfr10I RCCGGY 2 cut(s) 2254, 2308
Cfr13I GGNCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
ClaI ATCGAT 1 cut(s) 1647
CseI GACGC 1 cut(s) 2509
CspAI ACCGGT 1 cut(s) 2254
CspCI CAANNNNNGTGG 4 cut(s) 469, 504, 2065, 2100
DdeI CTNAG 9 cut(s) 180, 580, 986, 1278, 1463, 1655, 1699, 2340, 2533
DriI GACNNNNNGTC 1 cut(s) 1159
EaeI YGGCCR 2 cut(s) 188, 1997
Eam1105I GACNNNNNGTC 1 cut(s) 1159
Eco130I CCWWGG 2 cut(s) 656, 790
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
Eco47III AGCGCT 1 cut(s) 1741
EcoNI CCTNNNNNAGG 1 cut(s) 1499
EcoRII CCWGG 3 cut(s) 421, 1402, 2303
EcoT14I CCWWGG 2 cut(s) 656, 790
EcoT22I ATGCAT 3 cut(s) 523, 1051, 1393
ErhI CCWWGG 2 cut(s) 656, 790
Esp3I CGTCTC 1 cut(s) 230
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 1503, 1535
FaqI GGGAC 2 cut(s) 1017, 1648
FauI CCCGC 1 cut(s) 2132
FbaI TGATCA 1 cut(s) 696
FblI GTMKAC 1 cut(s) 1283
Fnu4HI GCNGC 8 cut(s) 12, 78, 87, 327, 594, 597, 1101, 1134
FokI GGATG 2 cut(s) 795, 2232
Fsp4HI GCNGC 8 cut(s) 12, 78, 87, 327, 594, 597, 1101, 1134
FspBI CTAG 5 cut(s) 945, 1082, 1412, 2004, 2493
GlaI GCGC 2 cut(s) 849, 1741
GluI GCNGC 8 cut(s) 12, 78, 87, 327, 594, 597, 1101, 1134
GsuI CTGGAG 2 cut(s) 669, 796
HaeII RGCGCY 1 cut(s) 1743
HaeIII GGCC 4 cut(s) 190, 1999, 2308, 2412
HapII CCGG 6 cut(s) 266, 1229, 1632, 1719, 2255, 2309
HgaI GACGC 1 cut(s) 2509
HhaI GCGC 2 cut(s) 850, 1742
Hin1I GRCGYC 1 cut(s) 2520
Hin6I GCGC 2 cut(s) 848, 1740
HinP1I GCGC 2 cut(s) 848, 1740
HincII GTYRAC 2 cut(s) 671, 1153
HindII GTYRAC 2 cut(s) 671, 1153
HindIII AAGCTT 5 cut(s) 320, 512, 1034, 2368, 2544
HinfI GANTC 8 cut(s) 578, 1118, 1280, 1529, 1797, 1832, 2033, 2276
HpaII CCGG 6 cut(s) 266, 1229, 1632, 1719, 2255, 2309
HphI GGTGA 2 cut(s) 1981, 2356
Hpy166II GTNNAC 8 cut(s) 671, 732, 765, 1153, 1284, 1343, 2260, 2378
Hpy188I TCNGA 4 cut(s) 171, 1165, 1840, 2275
Hpy8I GTNNAC 8 cut(s) 671, 732, 765, 1153, 1284, 1343, 2260, 2378
Hpy99I CGWCG 3 cut(s) 85, 128, 2525
HpyAV CCTTC 5 cut(s) 284, 816, 1529, 1920, 2103
HpyCH4IV ACGT 4 cut(s) 239, 369, 872, 2478
HpyF3I CTNAG 9 cut(s) 180, 580, 986, 1278, 1463, 1655, 1699, 2340, 2533
HpySE526I ACGT 4 cut(s) 239, 369, 872, 2478
Hsp92I GRCGYC 1 cut(s) 2520
HspAI GCGC 2 cut(s) 848, 1740
Ksp22I TGATCA 1 cut(s) 696
LmnI GCTCC 6 cut(s) 688, 967, 1685, 1706, 1912, 1917
Lsp1109I GCAGC 4 cut(s) 23, 583, 1087, 1120
LweI GCATC 8 cut(s) 530, 670, 949, 1036, 1156, 1230, 1378, 1894
MaeI CTAG 5 cut(s) 945, 1082, 1412, 2004, 2493
MaeII ACGT 4 cut(s) 239, 369, 872, 2478
MaeIII GTNAC 9 cut(s) 94, 257, 722, 1174, 1352, 1627, 1660, 1726, 2235
MbiI CCGCTC 1 cut(s) 2139
MboII GAAGA 8 cut(s) 46, 306, 473, 839, 1055, 1609, 1820, 1827
MfeI CAATTG 2 cut(s) 966, 1758
MflI RGATCY 7 cut(s) 802, 829, 1015, 1063, 1505, 2176, 2283
MlsI TGGCCA 2 cut(s) 190, 1999
MluNI TGGCCA 2 cut(s) 190, 1999
MlyI GAGTC 4 cut(s) 572, 1289, 1791, 2270
MmeI TCCRAC 4 cut(s) 1091, 1774, 2204, 2298
Mox20I TGGCCA 2 cut(s) 190, 1999
Mph1103I ATGCAT 3 cut(s) 523, 1051, 1393
MroXI GAANNNNTTC 1 cut(s) 2092
MscI TGGCCA 2 cut(s) 190, 1999
MslI CAYNNNNRTG 1 cut(s) 1777
Msp20I TGGCCA 2 cut(s) 190, 1999
MspA1I CMGCKG 1 cut(s) 1433
MspI CCGG 6 cut(s) 266, 1229, 1632, 1719, 2255, 2309
MspR9I CCNGG 4 cut(s) 423, 1404, 1633, 2305
MunI CAATTG 2 cut(s) 966, 1758
Mva1269I GAATGC 1 cut(s) 1391
MvaI CCWGG 3 cut(s) 423, 1404, 2305
MvnI CGCG 2 cut(s) 848, 850
NciI CCSGG 1 cut(s) 1633
NcoI CCATGG 1 cut(s) 656
NlaIV GGNNCC 2 cut(s) 788, 2508
NmuCI GTSAC 6 cut(s) 94, 257, 722, 1174, 1726, 2235
NsiI ATGCAT 3 cut(s) 523, 1051, 1393
NspI RCATGY 3 cut(s) 708, 892, 2457
NspV TTCGAA 1 cut(s) 436
PagI TCATGA 1 cut(s) 2555
PciI ACATGT 1 cut(s) 888
PctI GAATGC 1 cut(s) 1391
PdmI GAANNNNTTC 1 cut(s) 2092
PfeI GAWTC 4 cut(s) 1118, 1529, 1832, 2033
PfoI TCCNGGA 1 cut(s) 421
PinAI ACCGGT 1 cut(s) 2254
PkrI GCNGC 8 cut(s) 13, 79, 88, 328, 595, 598, 1102, 1135
PleI GAGTC 4 cut(s) 572, 1288, 1791, 2270
PpsI GAGTC 4 cut(s) 572, 1288, 1791, 2270
PscI ACATGT 1 cut(s) 888
PshBI ATTAAT 2 cut(s) 935, 1620
PsiI TTATAA 1 cut(s) 1559
Psp1406I AACGTT 2 cut(s) 872, 2478
Psp6I CCWGG 3 cut(s) 421, 1402, 2303
PspGI CCWGG 3 cut(s) 421, 1402, 2303
PspN4I GGNNCC 2 cut(s) 788, 2508
PspPI GGNCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 7 cut(s) 802, 829, 1015, 1063, 1505, 2176, 2283
PvuII CAGCTG 1 cut(s) 1433
RseI CAYNNNNRTG 1 cut(s) 1777
SatI GCNGC 8 cut(s) 12, 78, 87, 327, 594, 597, 1101, 1134
Sau96I GGNCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 4 cut(s) 572, 1289, 1791, 2270
ScrFI CCNGG 4 cut(s) 423, 1404, 1633, 2305
SfaNI GCATC 8 cut(s) 530, 670, 949, 1036, 1156, 1230, 1378, 1894
SfcI CTRYAG 3 cut(s) 1827, 1932, 2044
SfuI TTCGAA 1 cut(s) 436
SgrAI CRCCGGYG 1 cut(s) 2254
SinI GGWCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
SmiMI CAYNNNNRTG 1 cut(s) 1777
SmlI CTYRAG 1 cut(s) 2075
SmoI CTYRAG 1 cut(s) 2075
SpeI ACTAGT 2 cut(s) 944, 2003
SspI AATATT 1 cut(s) 574
SspMI CTAG 5 cut(s) 945, 1082, 1412, 2004, 2493
StyD4I CCNGG 4 cut(s) 421, 1402, 1631, 2303
StyI CCWWGG 2 cut(s) 656, 790
TaiI ACGT 4 cut(s) 242, 372, 875, 2481
TaqI TCGA 5 cut(s) 123, 436, 1647, 2428, 2489
TaqII GACCGA 1 cut(s) 782
TatI WGTACW 2 cut(s) 173, 2027
TauI GCSGC 4 cut(s) 80, 89, 329, 596
TfiI GAWTC 4 cut(s) 1118, 1529, 1832, 2033
TscAI CASTG 1 cut(s) 408
TseFI GTSAC 6 cut(s) 94, 257, 722, 1174, 1726, 2235
TseI GCWGC 4 cut(s) 11, 596, 1100, 1133
Tsp45I GTSAC 6 cut(s) 94, 257, 722, 1174, 1726, 2235
TspDTI ATGAA 5 cut(s) 149, 338, 515, 576, 2544
TspGWI ACGGA 3 cut(s) 148, 1204, 1484
TspRI CASTG 1 cut(s) 408
VpaK11BI GGWCC 6 cut(s) 362, 533, 765, 929, 1212, 1721
VspI ATTAAT 2 cut(s) 935, 1620
XagI CCTNNNNNAGG 1 cut(s) 1499
XapI RAATTY 5 cut(s) 311, 1090, 1267, 1374, 2360
XceI RCATGY 3 cut(s) 708, 892, 2457
XmiI GTMKAC 1 cut(s) 1283
XmnI GAANNNNTTC 1 cut(s) 2092
XspI CTAG 5 cut(s) 945, 1082, 1412, 2004, 2493
ZrmI AGTACT 1 cut(s) 175
Zsp2I ATGCAT 3 cut(s) 523, 1051, 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.