Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0006465

Physical Location & Seq

Genomic Coordinates Reverse (-)

65109310 .. 65114447

Gene Structure 5138 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG401300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2067 bp

ATGCAGCGGTTCACGGCCAAAATAGTTGAAATGATGAAGAATGAGAAGCTTTATGCATCTCAGGGAGGACCCATCATTTTATCCCAGATCGAGAACGAGTATGGAAACATTGATAAACCATATGGGCCTGCCGCCAAAACCTACATTAAGTGGGCAGCAAACATGGCTGTGTCTTTGGATACAGGGGTGCCCTGGGTCATGTGCCAGCAAGCAGATGCACCTTCTTCAGTGATCAACACTTGCAATGGATTTTACTGTGATCAATTCACCCCAAATTCTAACAGCACACCTAAAATTTGGACCGAGAATTGGAGTGGATGGTTTCTTTCCTTCGGTGGTGCTGTACCTCAGAGACCGGTGGAAGACCTTGCATTTGCTGTAGCACGCTTCTACCAGCGAGGTGGAACTTTCCAAAATTATTATATGTACCATGGTGGAACCAACTTTGGGCGGTCTAGTGGAGGACCTTTTATTGCTACAAGTTATGACTATGATGCTCCACTCGATGAGTATGGACTTCTTAGGCAGCCAAAGTGGGGTCATCTAAAAGATGTGCATAAGGCGATAAAGCTTTGTGAAGCTGCAATGGTGGCCACTGACCCAACAATTTCTTCTCTTGGTCAAAACATTGAGGCAGCTGTTTACAAAACAGGATCAGTATGTTCTGCTTTTCTTGCCAATGTCGACACCAAATCTGATGCCACTGTGACTTTCAATGGAAATTCATATCAATTGCCTGCATGGTCTGTGAGCATCTTACCTGACTGCAAAAATGTAGTGCTTAACACCGCAAAGATTAACACTGCGGCTATGATTCCAAGCTTCATGCGTCAATCAATTTCAGCTGATGTTGAGCCCACTGGAGCCGTGGGCTCAGGCTGGAGTTGGATAAATGAACCAGTGGGTATCTCAAAGGCTGATGCATTTACTAGAAAGGGATTATTGGAGCAGATAAACACTACAGCTGATAAAAGTGACTATTTATGGTACTCTACAAGCATTGATGTCAAAGATGGATATAAAGCTGATCTTCACGTGGAATCTCTTGGCCATGCTCTTCATGCTTTTGTCAATGGAAAGCTTGCAGGGAGTGGAACAGGCAACAGTGGCAATGCCAAGGTTTCTGTGGACATCCCTGTCGAATTCGCATCTGGGAAGAACAAAATTGATCTTCTGAGTCTGACTGTTGGGCTTCAGAATTATGGATCTTTCTTTGACTTGGTGGGTGCTGGAATCACTGGTCCTGTACAACTGAAAGGCTCAACAAATGGCACTACTATTGATCTTTCTTCTCAACAATGGACATATCAGATTGGACTTAAAGGTGAAGATGAAGATCTTCCCAGTGGAAGTTCTCAGTGGATTTCACAACCTACCTTGCCCAAGAATCAGCCATTGACGTGGTACAAGACCCAATTTGATGCCCCCGGTGGCAGTAACCCTGTTGCACTAGACTTCACTGGAATGGGGAAGGGTGAGGCATGGGTGAATGGACAAAGCATTGGACGTTACTGGCCCACTAATATTGCTCCAAAAAGTGGTTGCCCTGACACTTGCAATTACAGAGGAGCTTATAGTGCAGACAAATGCCGAAAGAACTGTGGCAAGCCATCTCAAGAATTGTACCACGTTCCTCGTTCATGGCTGAAATCCAGTGGCAATATTCTTGTATTATTTGAAGAAGTTGGGGGTGATCCGACACAGTTATCTTTTGCCACGAGACAGGTAGAAAGTTTGTGCTCTCATGTATCAGAGTCTCACCCATCACCTGTAGATATGTGGAGTTCAGATTCAAAAGCAGGAAGGAAGTCACGGCCGAGGCTATCACTTGAGTGCCCATTTCCTAATCAAGTCATTTCTTCAATCAAGTTTGCAAGTTACGGAAGACCTAGTGGGACTTGTGGGAGCTTTAGCCATGGCTCGTGCAGGAGCCGTAGCGCTCTATCCATTGTACAGAAGGCGTGCATTGGATCAAAGAGTTGTAGCATCGAAGTCTCGACTAATACATTTGGTGACCCATGTAAAGGAGTAGCAAAGAGTTTAGCAGTAGAAGTTTCTTGTAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

688

Amino Acids

74.25

Weight (kDa)

7.75

Isoelectric Point (pI)

33.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	1 - 188	5.5e-59	Glycosyl hydrolases family 35
GHD	PF17834	212 - 266	8.3e-24	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	466 - 554	7.1e-26	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	610 - 687	6.6e-22	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28470 AT2G28470 AT2G28470 AT2G28470 AT2G28470
fragaria_vesca	FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630
malus_domestica	MD03G1114300.v1.1 MD11G1133400.v1.1
prunus_persica	Prupe.6G099700_v2.0.a1 Prupe.6G099700_v2.0.a1 Prupe.6G099700_v2.0.a1
pyrus_communis	pycom11g11010
rosa_chinensis	RchiOBHm_Chr5g0059701
rosa_laevigata	RLG00000035321
rosa_multiflora	Rmu_sc0000660.1_g000045
rosa_roxburghii	Rroxscaffold_1G00020930
rosa_rugosa	Rorug05G0324200
rosa_samantha	Rh5AG389700 Rh5BG401300 Rh5DG417700
rosa_wichuraiana	Rw5G036670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1136
AccB1I	GGYRCC	1 cut(s)	187
AccB7I	CCANNNNNTGG	1 cut(s)	1538
AccI	GTMKAC	1 cut(s)	684
AciI	CCGC	5 cut(s)	7, 132, 451, 789, 806
AclWI	GGATC	4 cut(s)	661, 1213, 1688, 1978
AcoI	YGGCCR	4 cut(s)	15, 591, 1048, 1814
AcsI	RAATTY	4 cut(s)	274, 294, 721, 1142
AcuI	CTGAAG	2 cut(s)	210, 1178
AcvI	CACGTG	1 cut(s)	1036
AfaI	GTAC	7 cut(s)	345, 428, 989, 1248, 1406, 1625, 1953
AfeI	AGCGCT	1 cut(s)	1939
AfiI	CCNNNNNNNGG	5 cut(s)	309, 447, 536, 1538, 2024
AgeI	ACCGGT	1 cut(s)	355
AgsI	TTSAA	5 cut(s)	29, 715, 1679, 1794, 1863
AjiI	CACGTC	1 cut(s)	1401
AjnI	CCWGG	1 cut(s)	191
AjuI	GAANNNNNNNTTGG	2 cut(s)	595, 627
AleI	CACNNNNGTG	1 cut(s)	1831
Alw21I	GWGCWC	1 cut(s)	1742
Alw26I	GTCTC	4 cut(s)	346, 1714, 1761, 1999
AlwI	GGATC	4 cut(s)	661, 1213, 1688, 1978
Aor51HI	AGCGCT	1 cut(s)	1939
AoxI	GGCC	6 cut(s)	15, 125, 591, 1048, 1514, 1814
ApeKI	GCWGC	5 cut(s)	4, 155, 526, 581, 635
ApoI	RAATTY	4 cut(s)	274, 294, 721, 1142
ArsI	GACNNNNNNTTYG	2 cut(s)	356, 388
AsiGI	ACCGGT	1 cut(s)	355
Asp700I	GAANNNNTTC	1 cut(s)	1338
AspLEI	GCGC	1 cut(s)	1940
AspS9I	GGNCC	6 cut(s)	68, 125, 300, 464, 1241, 1515
AsuC2I	CCSGG	1 cut(s)	1428
AsuHPI	GGTGA	8 cut(s)	259, 1337, 1487, 1498, 1703, 1751, 1758, 2024
AvaII	GGWCC	4 cut(s)	68, 300, 464, 1241
BaeGI	GKGCMC	2 cut(s)	192, 1838
BaeI	ACNNNNGTAYC	2 cut(s)	171, 204
BalI	TGGCCA	2 cut(s)	593, 1050
BanI	GGYRCC	1 cut(s)	187
BanII	GRGCYC	2 cut(s)	858, 875
BauI	CACGAG	2 cut(s)	1717, 1921
BbrPI	CACGTG	1 cut(s)	1036
BbsI	GAAGAC	2 cut(s)	369, 1891
Bbv12I	GWGCWC	1 cut(s)	1742
BbvI	GCAGC	5 cut(s)	16, 167, 538, 568, 647
BccI	CCATC	5 cut(s)	80, 312, 1007, 1618, 1771
BceAI	ACGGC	4 cut(s)	30, 851, 1829, 1917
BciT130I	CCWGG	1 cut(s)	193
BciVI	GTATCC	1 cut(s)	172
BclI	TGATCA	2 cut(s)	231, 259
BcnI	CCSGG	1 cut(s)	1428
BcoDI	GTCTC	4 cut(s)	346, 1714, 1761, 1999
BfaI	CTAG	4 cut(s)	456, 930, 1451, 1890
BfmI	CTRYAG	3 cut(s)	378, 960, 1770
BfoI	RGCGCY	1 cut(s)	1941
BfuI	GTATCC	1 cut(s)	172
BglII	AGATCT	1 cut(s)	1336
BisI	GCNGC	7 cut(s)	5, 132, 156, 527, 582, 636, 807
BlsI	GCNGC	7 cut(s)	6, 133, 157, 528, 583, 637, 808
Bme1390I	CCNGG	2 cut(s)	193, 1428
Bme18I	GGWCC	4 cut(s)	68, 300, 464, 1241
BmgBI	CACGTC	1 cut(s)	1401
BmgT120I	GGNCC	6 cut(s)	68, 125, 300, 464, 1241, 1515
BmiI	GGNNCC	5 cut(s)	70, 189, 439, 865, 1931
BmrFI	CCNGG	2 cut(s)	193, 1428
BmrI	ACTGGG	1 cut(s)	1338
BmsI	GCATC	9 cut(s)	65, 205, 484, 688, 762, 910, 1157, 1411, 1995
BmuI	ACTGGG	1 cut(s)	1338
BpiI	GAAGAC	2 cut(s)	369, 1891
BpmI	CTGGAG	2 cut(s)	882, 901
Bpu10I	CCTNAGC	1 cut(s)	874
BpuEI	CTTGAG	2 cut(s)	1599, 1850
BpuMI	CCSGG	1 cut(s)	1428
BsaAI	YACGTR	1 cut(s)	1036
BsaBI	GATNNNNATC	1 cut(s)	1335
BsaI	GGTCTC	1 cut(s)	346
BsaJI	CCNNGG	8 cut(s)	191, 192, 430, 867, 1116, 1426, 1817, 1915
BsaWI	WCCGGW	1 cut(s)	355
Bsc4I	CCNNNNNNNGG	5 cut(s)	309, 447, 536, 1538, 2024
Bse118I	RCCGGY	1 cut(s)	355
Bse1I	ACTGG	7 cut(s)	865, 899, 1243, 1344, 1465, 1517, 1653
Bse3DI	GCAATG	3 cut(s)	250, 591, 1117
Bse8I	GATNNNNATC	1 cut(s)	1335
BseBI	CCWGG	1 cut(s)	193
BseDI	CCNNGG	8 cut(s)	191, 192, 430, 867, 1116, 1426, 1817, 1915
BseGI	GGATG	2 cut(s)	323, 1131
BseJI	GATNNNNATC	1 cut(s)	1335
BseLI	CCNNNNNNNGG	5 cut(s)	309, 447, 536, 1538, 2024
BseMI	GCAATG	3 cut(s)	250, 591, 1117
BseMII	CTCAG	5 cut(s)	74, 362, 888, 1166, 1370
BseNI	ACTGG	7 cut(s)	865, 899, 1243, 1344, 1465, 1517, 1653
BseRI	GAGGAG	1 cut(s)	1581
BseSI	GKGCMC	2 cut(s)	192, 1838
BseX3I	CGGCCG	1 cut(s)	1814
BseXI	GCAGC	5 cut(s)	16, 167, 538, 568, 647
BsgI	GTGCAG	2 cut(s)	1599, 1945
Bsh1285I	CGRYCG	1 cut(s)	1817
BshFI	GGCC	6 cut(s)	17, 127, 593, 1050, 1516, 1816
BshNI	GGYRCC	1 cut(s)	187
BshTI	ACCGGT	1 cut(s)	355
BsiEI	CGRYCG	1 cut(s)	1817
BsiHKAI	GWGCWC	1 cut(s)	1742
BsiSI	CCGG	2 cut(s)	356, 1428
BslFI	GGGAC	1 cut(s)	1909
BslI	CCNNNNNNNGG	5 cut(s)	309, 447, 536, 1538, 2024
BsmAI	GTCTC	4 cut(s)	346, 1714, 1761, 1999
BsmFI	GGGAC	1 cut(s)	1909
BsnI	GGCC	6 cut(s)	17, 127, 593, 1050, 1516, 1816
Bso31I	GGTCTC	1 cut(s)	346
Bsp1286I	GDGCHC	5 cut(s)	192, 858, 875, 1742, 1838
Bsp1407I	TGTACA	2 cut(s)	1246, 1951
Bsp19I	CCATGG	2 cut(s)	430, 1915
BspACI	CCGC	5 cut(s)	7, 132, 451, 789, 806
BspANI	GGCC	6 cut(s)	17, 127, 593, 1050, 1516, 1816
BspCNI	CTCAG	5 cut(s)	73, 361, 887, 1167, 1369
BspLI	GGNNCC	5 cut(s)	70, 189, 439, 865, 1931
BspPI	GGATC	4 cut(s)	661, 1213, 1688, 1978
BspQI	GCTCTTC	1 cut(s)	1062
BspT107I	GGYRCC	1 cut(s)	187
BspTNI	GGTCTC	1 cut(s)	346
BsrDI	GCAATG	3 cut(s)	250, 591, 1117
BsrFI	RCCGGY	1 cut(s)	355
BsrGI	TGTACA	2 cut(s)	1246, 1951
BsrI	ACTGG	7 cut(s)	865, 899, 1243, 1344, 1465, 1517, 1653
BssAI	RCCGGY	1 cut(s)	355
BssECI	CCNNGG	8 cut(s)	191, 192, 430, 867, 1116, 1426, 1817, 1915
BssSI	CACGAG	2 cut(s)	1717, 1921
BssT1I	CCWWGG	3 cut(s)	430, 1116, 1915
Bst2BI	CACGAG	2 cut(s)	1717, 1921
Bst2UI	CCWGG	1 cut(s)	193
Bst4CI	ACNGT	6 cut(s)	257, 706, 1106, 1186, 1601, 1704
Bst6I	CTCTTC	1 cut(s)	1062
BstAUI	TGTACA	2 cut(s)	1246, 1951
BstBAI	YACGTR	1 cut(s)	1036
BstC8I	GCNNGC	8 cut(s)	129, 206, 210, 385, 738, 1083, 1607, 1963
BstDEI	CTNAG	6 cut(s)	60, 348, 521, 874, 1175, 1356
BstDSI	CCRYGG	3 cut(s)	430, 867, 1915
BstEII	GGTNACC	1 cut(s)	2012
BstF5I	GGATG	2 cut(s)	323, 1131
BstH2I	RGCGCY	1 cut(s)	1941
BstHHI	GCGC	1 cut(s)	1940
BstMAI	GTCTC	4 cut(s)	346, 1714, 1761, 1999
BstMCI	CGRYCG	1 cut(s)	1817
BstMWI	GCNNNNNNNGC	6 cut(s)	164, 590, 674, 1061, 1107, 1577
BstNI	CCWGG	1 cut(s)	193
BstPI	GGTNACC	1 cut(s)	2012
BstSCI	CCNGG	2 cut(s)	191, 1426
BstSFI	CTRYAG	3 cut(s)	378, 960, 1770
BstSLI	GKGCMC	2 cut(s)	192, 1838
BstV1I	GCAGC	5 cut(s)	16, 167, 538, 568, 647
BstV2I	GAAGAC	2 cut(s)	369, 1891
BstX2I	RGATCY	2 cut(s)	1205, 1336
BstXI	CCANNNNNNTGG	2 cut(s)	401, 1401
BstYI	RGATCY	2 cut(s)	1205, 1336
BstZI	CGGCCG	1 cut(s)	1814
BsuI	GTATCC	1 cut(s)	172
BsuRI	GGCC	6 cut(s)	17, 127, 593, 1050, 1516, 1816
BtgI	CCRYGG	3 cut(s)	430, 867, 1915
BtrI	CACGTC	1 cut(s)	1401
BtsCI	GGATG	2 cut(s)	323, 1131
BtsI	GCAGTG	1 cut(s)	801
Cac8I	GCNNGC	8 cut(s)	129, 206, 210, 385, 738, 1083, 1607, 1963
CfoI	GCGC	1 cut(s)	1940
Cfr10I	RCCGGY	1 cut(s)	355
Cfr13I	GGNCC	6 cut(s)	68, 125, 300, 464, 1241, 1515
CseI	GACGC	1 cut(s)	818
Csp6I	GTAC	7 cut(s)	344, 427, 988, 1247, 1405, 1624, 1952
CspAI	ACCGGT	1 cut(s)	355
CspCI	CAANNNNNGTGG	2 cut(s)	1507, 1542
CviQI	GTAC	7 cut(s)	344, 427, 988, 1247, 1405, 1624, 1952
DdeI	CTNAG	6 cut(s)	60, 348, 521, 874, 1175, 1356
DrdI	GACNNNNNNGTC	1 cut(s)	1136
DseDI	GACNNNNNNGTC	1 cut(s)	1136
EaeI	YGGCCR	4 cut(s)	15, 591, 1048, 1814
EagI	CGGCCG	1 cut(s)	1814
Eam1104I	CTCTTC	1 cut(s)	1062
EarI	CTCTTC	1 cut(s)	1062
EclXI	CGGCCG	1 cut(s)	1814
Eco130I	CCWWGG	3 cut(s)	430, 1116, 1915
Eco24I	GRGCYC	2 cut(s)	858, 875
Eco31I	GGTCTC	1 cut(s)	346
Eco47I	GGWCC	4 cut(s)	68, 300, 464, 1241
Eco47III	AGCGCT	1 cut(s)	1939
Eco52I	CGGCCG	1 cut(s)	1814
Eco57I	CTGAAG	2 cut(s)	210, 1178
Eco72I	CACGTG	1 cut(s)	1036
Eco91I	GGTNACC	1 cut(s)	2012
EcoO109I	RGGNCCY	2 cut(s)	68, 464
EcoO65I	GGTNACC	1 cut(s)	2012
EcoRI	GAATTC	1 cut(s)	1142
EcoRII	CCWGG	1 cut(s)	191
EcoT14I	CCWWGG	3 cut(s)	430, 1116, 1915
EcoT22I	ATGCAT	2 cut(s)	58, 925
EcoT38I	GRGCYC	2 cut(s)	858, 875
ErhI	CCWWGG	3 cut(s)	430, 1116, 1915
FalI	AAGNNNNNCTT	2 cut(s)	1014, 1046
FaqI	GGGAC	1 cut(s)	1909
FauNDI	CATATG	1 cut(s)	121
FbaI	TGATCA	2 cut(s)	231, 259
FblI	GTMKAC	1 cut(s)	684
Fnu4HI	GCNGC	7 cut(s)	5, 132, 156, 527, 582, 636, 807
FokI	GGATG	2 cut(s)	330, 1118
FriOI	GRGCYC	2 cut(s)	858, 875
Fsp4HI	GCNGC	7 cut(s)	5, 132, 156, 527, 582, 636, 807
FspBI	CTAG	4 cut(s)	456, 930, 1451, 1890
GlaI	GCGC	1 cut(s)	1939
GluI	GCNGC	7 cut(s)	5, 132, 156, 527, 582, 636, 807
GsuI	CTGGAG	2 cut(s)	882, 901
HaeII	RGCGCY	1 cut(s)	1941
HaeIII	GGCC	6 cut(s)	17, 127, 593, 1050, 1516, 1816
HapII	CCGG	2 cut(s)	356, 1428
HgaI	GACGC	1 cut(s)	818
HhaI	GCGC	1 cut(s)	1940
Hin6I	GCGC	1 cut(s)	1938
HinP1I	GCGC	1 cut(s)	1938
HincII	GTYRAC	1 cut(s)	685
HindII	GTYRAC	1 cut(s)	685
HindIII	AAGCTT	4 cut(s)	47, 569, 820, 1079
HinfI	GANTC	7 cut(s)	814, 1040, 1177, 1233, 1387, 1754, 1790
HpaII	CCGG	2 cut(s)	356, 1428
HphI	GGTGA	8 cut(s)	259, 1337, 1487, 1498, 1703, 1751, 1758, 2024
Hpy166II	GTNNAC	4 cut(s)	12, 643, 685, 1129
Hpy188I	TCNGA	9 cut(s)	351, 697, 1176, 1182, 1197, 1311, 1698, 1753, 1789
Hpy188III	TCNNGA	3 cut(s)	91, 1616, 1996
Hpy8I	GTNNAC	4 cut(s)	12, 643, 685, 1129
HpyAV	CCTTC	5 cut(s)	231, 340, 1465, 1797, 1951
HpyCH4III	ACNGT	6 cut(s)	257, 706, 1106, 1186, 1601, 1704
HpyCH4IV	ACGT	4 cut(s)	1035, 1400, 1507, 1629
HpyF10VI	GCNNNNNNNGC	6 cut(s)	164, 590, 674, 1061, 1107, 1577
HpyF3I	CTNAG	6 cut(s)	60, 348, 521, 874, 1175, 1356
HpySE526I	ACGT	4 cut(s)	1035, 1400, 1507, 1629
HspAI	GCGC	1 cut(s)	1938
Ksp22I	TGATCA	2 cut(s)	231, 259
LguI	GCTCTTC	1 cut(s)	1062
LmnI	GCTCC	7 cut(s)	502, 863, 946, 1534, 1568, 1905, 1929
Lsp1109I	GCAGC	5 cut(s)	16, 167, 538, 568, 647
LweI	GCATC	9 cut(s)	65, 205, 484, 688, 762, 910, 1157, 1411, 1995
MaeI	CTAG	4 cut(s)	456, 930, 1451, 1890
MaeII	ACGT	4 cut(s)	1035, 1400, 1507, 1629
MaeIII	GTNAC	7 cut(s)	706, 974, 1436, 1508, 1809, 1877, 2012
MfeI	CAATTG	1 cut(s)	731
MflI	RGATCY	2 cut(s)	1205, 1336
MhlI	GDGCHC	5 cut(s)	192, 858, 875, 1742, 1838
MlsI	TGGCCA	2 cut(s)	593, 1050
MluNI	TGGCCA	2 cut(s)	593, 1050
MlyI	GAGTC	2 cut(s)	1186, 1763
MmeI	TCCRAC	2 cut(s)	866, 1721
MnlI	CCTC	9 cut(s)	59, 357, 392, 455, 625, 1471, 1559, 1644, 1812
Mox20I	TGGCCA	2 cut(s)	593, 1050
Mph1103I	ATGCAT	2 cut(s)	58, 925
MroXI	GAANNNNTTC	1 cut(s)	1338
MscI	TGGCCA	2 cut(s)	593, 1050
MseI	TTAA	4 cut(s)	147, 783, 798, 1320
MslI	CAYNNNNRTG	4 cut(s)	167, 1399, 1463, 1831
Msp20I	TGGCCA	2 cut(s)	593, 1050
MspA1I	CMGCKG	4 cut(s)	7, 638, 845, 965
MspI	CCGG	2 cut(s)	356, 1428
MspR9I	CCNGG	2 cut(s)	193, 1428
MunI	CAATTG	1 cut(s)	731
MvaI	CCWGG	1 cut(s)	193
MwoI	GCNNNNNNNGC	6 cut(s)	164, 590, 674, 1061, 1107, 1577
NciI	CCSGG	1 cut(s)	1428
NcoI	CCATGG	2 cut(s)	430, 1915
NdeI	CATATG	1 cut(s)	121
NlaIV	GGNNCC	5 cut(s)	70, 189, 439, 865, 1931
NmeAIII	GCCGAG	1 cut(s)	1842
NmuCI	GTSAC	4 cut(s)	706, 974, 1809, 2012
NsiI	ATGCAT	2 cut(s)	58, 925
OliI	CACNNNNGTG	1 cut(s)	1831
PasI	CCCWGGG	1 cut(s)	192
PciSI	GCTCTTC	1 cut(s)	1062
PdmI	GAANNNNTTC	1 cut(s)	1338
PfeI	GAWTC	5 cut(s)	814, 1040, 1233, 1387, 1790
PflMI	CCANNNNNTGG	1 cut(s)	1538
PinAI	ACCGGT	1 cut(s)	355
PkrI	GCNGC	7 cut(s)	6, 133, 157, 528, 583, 637, 808
PleI	GAGTC	2 cut(s)	1185, 1762
PmaCI	CACGTG	1 cut(s)	1036
PmlI	CACGTG	1 cut(s)	1036
PpsI	GAGTC	2 cut(s)	1185, 1762
Ppu21I	YACGTR	1 cut(s)	1036
PpuMI	RGGWCCY	2 cut(s)	68, 464
Psp5II	RGGWCCY	2 cut(s)	68, 464
Psp6I	CCWGG	1 cut(s)	191
PspCI	CACGTG	1 cut(s)	1036
PspEI	GGTNACC	1 cut(s)	2012
PspGI	CCWGG	1 cut(s)	191
PspN4I	GGNNCC	5 cut(s)	70, 189, 439, 865, 1931
PspPI	GGNCC	6 cut(s)	68, 125, 300, 464, 1241, 1515
PspPPI	RGGWCCY	2 cut(s)	68, 464
PsuI	RGATCY	2 cut(s)	1205, 1336
PvuII	CAGCTG	3 cut(s)	638, 845, 965
RsaI	GTAC	7 cut(s)	345, 428, 989, 1248, 1406, 1625, 1953
RsaNI	GTAC	7 cut(s)	344, 427, 988, 1247, 1405, 1624, 1952
RseI	CAYNNNNRTG	4 cut(s)	167, 1399, 1463, 1831
SalI	GTCGAC	1 cut(s)	683
SapI	GCTCTTC	1 cut(s)	1062
SaqAI	TTAA	4 cut(s)	147, 783, 798, 1320
SatI	GCNGC	7 cut(s)	5, 132, 156, 527, 582, 636, 807
Sau96I	GGNCC	6 cut(s)	68, 125, 300, 464, 1241, 1515
SchI	GAGTC	2 cut(s)	1186, 1763
ScrFI	CCNGG	2 cut(s)	193, 1428
SduI	GDGCHC	5 cut(s)	192, 858, 875, 1742, 1838
SfaNI	GCATC	9 cut(s)	65, 205, 484, 688, 762, 910, 1157, 1411, 1995
SfcI	CTRYAG	3 cut(s)	378, 960, 1770
SinI	GGWCC	4 cut(s)	68, 300, 464, 1241
SmiMI	CAYNNNNRTG	4 cut(s)	167, 1399, 1463, 1831
SmlI	CTYRAG	2 cut(s)	1614, 1829
SmoI	CTYRAG	2 cut(s)	1614, 1829
SsiI	CCGC	5 cut(s)	7, 132, 451, 789, 806
SspI	AATATT	2 cut(s)	1525, 1663
SspMI	CTAG	4 cut(s)	456, 930, 1451, 1890
StyD4I	CCNGG	2 cut(s)	191, 1426
StyI	CCWWGG	3 cut(s)	430, 1116, 1915
TaaI	ACNGT	6 cut(s)	257, 706, 1106, 1186, 1601, 1704
TaiI	ACGT	4 cut(s)	1038, 1403, 1510, 1632
TaqI	TCGA	6 cut(s)	90, 504, 684, 1140, 1989, 1997
TaqII	GACCGA	1 cut(s)	317
TatI	WGTACW	2 cut(s)	1246, 1951
TauI	GCSGC	2 cut(s)	134, 809
TfiI	GAWTC	5 cut(s)	814, 1040, 1233, 1387, 1790
Tru1I	TTAA	4 cut(s)	147, 783, 798, 1320
Tru9I	TTAA	4 cut(s)	147, 783, 798, 1320
TseFI	GTSAC	4 cut(s)	706, 974, 1809, 2012
TseI	GCWGC	5 cut(s)	4, 155, 526, 581, 635
Tsp45I	GTSAC	4 cut(s)	706, 974, 1809, 2012
TspDTI	ATGAA	7 cut(s)	50, 714, 814, 909, 1049, 1347, 1629
TspGWI	ACGGA	1 cut(s)	1896
Van91I	CCANNNNNTGG	1 cut(s)	1538
VpaK11BI	GGWCC	4 cut(s)	68, 300, 464, 1241
XapI	RAATTY	4 cut(s)	274, 294, 721, 1142
XcmI	CCANNNNNNNNNTGG	2 cut(s)	865, 1123
XmiI	GTMKAC	1 cut(s)	684
XmnI	GAANNNNTTC	1 cut(s)	1338
XspI	CTAG	4 cut(s)	456, 930, 1451, 1890
Zsp2I	ATGCAT	2 cut(s)	58, 925

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.