MD03G1114300.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006465
Physical Location & Seq
Reverse (-)
9987858 .. 9994620
6763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1114300.v1.1.491

Sequence Viewer

Length: 2529 bp
ATGAGGATTTTAGTGGTGGTTTTGGTGCTCGGAGTTCTGGCGACGGCGTCGTATTGCGCCACCGTGACATACGACCACAGGGCGCTGGTGATTGATGGGAAGCGCCGAGTTTTGGTGTCTGGCTCCATTCACTATCCTCGCAGCACTCCTGAGATGTGGCCGGACCTTATCCAAAAATCGAAAGACGGAGGCTTGGACGTGATCGAGACCTACGTCTTCTGGAACTTGCACGAACCAGTTCAAGGCCAGTATGATTTTGGGGGAAGAAAAGATTTGGTTAAATTTGTGAAGACAGTAGCAGAAGCTGGCCTTTATGTGCACCTTCGAATAGGTCCATATGTGTGCGCAGAATGGAACTATGGTGGCTTTCCTCTTTGGTTGCATTTCATACCCGGAATTCAATTGCGAACGGACAATGAGCCGTTCAAGGCAGAAATGCAGCGGTTCACAGCAAAAATTGTTGATATGATGAAGAAGGAGAAGCTTTATGCATCTCAGGGAGGACCCATCATTTTATCCCAGATTGAAAACGAGTATGGTAACATTGATCAAGCATATGGGGCCGCTGCCCAAACCTATATTAAGTGGGCAGCAAGCATGGCTGTGTCTTTGGATACAGGGGTTCCCTGGGTCATGTGTCAGCAAGCCGACGCTCCTGCATCTGTGATCAGCACTTGCAATGGATTTTATTGTGATCAATGGACCCCAAGACTTCCTGAAAAGAGACCCAAAATGTGGACCGAGAATTGGAGTGGATGGTTTCTTTCCTTTGGTGGTGCTGTGCCTCAAAGACCAGTGGAAGACCTCGCATTTGCCGTAGCACGCTTCTTTCAGAAAGGTGGAACTTTCCAAAATTATTATATGTACCATGGCGGGACTAACTTTGGGCGGAGTGCTGGTGGACCCTTTATTGCTACAAGTTATGACTACGATGCTCCAATTGATGAGTATGGATTTCTTAGGCAACCTAAGTGGGGTCACCTAAAAGATGTGCATAAGGCCGTAAAGCTTTGTGAAGAAGCAATGGTGGCCACTGACCCAAAAAATTCTTCTCTTGGTCCAAACGTGGAGGCAACTGTTTACCAAACAGGATCAGCATGTGCTGCGTTTCTTTCCAATTCTGACACTAAATCTGATGCCACTGTGACGTTCAATGGAAATTCTTATCAGTTGCCTGCATGGTCTGTGAGCATCTTACCAGACTGCAAAAATGTAGCGCTTAACACTGCAAAGATTAACTCTGCTGCCATGATTCCGAGCTTCATGCATCACTCAGTGATAGATGATATTGATTCCTCTGAAGCATTGGGCTCAGGCTGGAGTTGGATAAATGAACCAGTTGGTATCTCAAAGAAGGATGCATTTGCCAGAGTTGGATTGTTGGAGCAAATAAATACCACAGCTGATAAAAGTGACTATTTATGGTACTCTTTAAGCATTGATGTCACAAGTTCTGATACGTTCCTTCAAGATGGGTCTCAAACTGTTCTTCATGTGGAATCACTTGGCCATGCCCTTCATGCGTTTATTAATGGAAAGCCTGCAGGGAGTGGAACAGGCAGCGCTAGCAATCCCACGGTTTCTGTGGAGATCCCTGTAACATTTGCATCTGGGAAGAACACAATTGATCTTCTGAGTTTGACTGTTGGCCTTCAGAACTATGGAGCTTTTTTTGACAAAACAGGTGCTGGTATCACTGGTCCTGTACAGCTGAAAGGCTTGAAAAATGGCACTACTATTGATCTCTCATCTCAGCGGTGGACATATCAGATTGGACTTCAAGGGGAAGACTTGGATTTTCCCAGTGGAAGTTCTTCTCAGTGGGTTTCACAACCTACCTTGCCCAAGAAACAACCCTTGACATGGTACAAGGCAACATTTAATGCTCCTGATGGCAGTAACCCTGTTGCACTAGACTTTACAGGGATGGGAAAGGGTGAAGCATGGGTGAACGGACAAAGTATTGGGCGGTACTGGCCGACCAACATTTCTCCAACTAGTGGTTGCCCTGATTCTTGTGATTTCAGGGGACCTTATAACTCCGACAAATGCAGAAAGAACTGTGGCAAGCCATCTCAGGAATTGTACCACGTGCCTCGTTCATGGTTGAAACCAAGTGACAATACTCTTGTCCTGTTTGAGGAAATTGGGGGTGATCCAACGCAGATATCTTTTGCTACCAGACAGATTGAAAGTTTGTGCTCACATGTATCGGAGTCTCACCTGTCACCTGTAGATACATGGAGTTCAGATTCAAAAGTGGGAAGGAAGTTAGGGCCTGTGCTATCACTTGAGTGCCCATTTCCTAACCAGGTCATTTCTTCAATCAAATTTGCAAGTTACGGAAAACCTCAAGGGACTTGTGGGAGCTTTAGCCATGGCCAATGCAAGAGCAGTAGCGCTCTATCCATTGTACAGAAGGCTTGTGTTGGATCCAAGAGCTGTAGCGTCGAAGTCTCGGTTAAAACATTCGGTGACCCATGTAATGGAGTAGCAAAGAGTTTAGCAGTAGAAGCATCCTGTAGATAG

Protein Analysis

843

Amino Acids

91.7

Weight (kDa)

6.3

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 29 - 334 1.4e-123 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 50 - 197 3.3e-07 Beta-galactosidase
GHD PF17834 357 - 412 1.1e-24 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 620 - 708 2.2e-26 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 764 - 841 4.9e-21 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2037
Acc16I TGCGCA 1 cut(s) 346
AccB7I CCANNNNNTGG 3 cut(s) 735, 2000, 2486
AciI CCGC 6 cut(s) 442, 564, 873, 889, 1756, 1969
AclWI GGATC 5 cut(s) 1099, 1585, 2150, 2427, 2440
AcoI YGGCCR 5 cut(s) 158, 1029, 1507, 1976, 2380
AcsI RAATTY 5 cut(s) 281, 396, 1045, 1159, 2330
AcuI CTGAAG 2 cut(s) 1320, 1637
AcvI CACGTG 1 cut(s) 2092
AcyI GRCGYC 1 cut(s) 47
AdeI CACNNNGTG 1 cut(s) 1276
AfaI GTAC 7 cut(s) 866, 1427, 1707, 1868, 1973, 2087, 2415
AfeI AGCGCT 3 cut(s) 1218, 1564, 2401
AfiI CCNNNNNNNGG 9 cut(s) 112, 242, 735, 747, 974, 1863, 2000, 2140, 2486
AflIII ACRYGT 1 cut(s) 2206
AhlI ACTAGT 1 cut(s) 1997
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 2 cut(s) 626, 2310
AjuI GAANNNNNNNTTGG 2 cut(s) 1033, 1065
AleI CACNNNNGTG 1 cut(s) 2293
AluBI AGCT 9 cut(s) 305, 484, 1009, 1260, 1403, 1667, 1711, 2370, 2442
AluI AGCT 9 cut(s) 305, 484, 1009, 1260, 1403, 1667, 1711, 2370, 2442
Alw21I GWGCWC 3 cut(s) 30, 321, 2204
Alw26I GTCTC 5 cut(s) 200, 718, 1482, 2223, 2461
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 5 cut(s) 1099, 1585, 2150, 2427, 2440
AlwNI CAGNNNCTG 2 cut(s) 305, 1688
Aor51HI AGCGCT 3 cut(s) 1218, 1564, 2401
ApaLI GTGCAC 1 cut(s) 317
ApeKI GCWGC 7 cut(s) 141, 439, 566, 590, 1103, 1244, 1560
ApoI RAATTY 5 cut(s) 281, 396, 1045, 1159, 2330
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
AseI ATTAAT 1 cut(s) 1530
Asp700I GAANNNNTTC 2 cut(s) 237, 1813
AspLEI GCGC 7 cut(s) 59, 85, 105, 347, 1219, 1565, 2402
AsuC2I CCSGG 1 cut(s) 393
AsuHPI GGTGA 8 cut(s) 100, 971, 1949, 1960, 2165, 2213, 2220, 2486
AsuII TTCGAA 1 cut(s) 325
AsuNHI GCTAGC 1 cut(s) 1565
AvaII GGWCC 9 cut(s) 163, 332, 503, 702, 738, 902, 1058, 1700, 2030
BaeGI GKGCMC 2 cut(s) 321, 2300
BaeI ACNNNNGTAYC 2 cut(s) 606, 639
BalI TGGCCA 3 cut(s) 1031, 1509, 2382
BamHI GGATCC 1 cut(s) 2432
BanII GRGCYC 1 cut(s) 1313
BbrPI CACGTG 1 cut(s) 2092
BbsI GAAGAC 4 cut(s) 208, 296, 807, 1794
Bbv12I GWGCWC 3 cut(s) 30, 321, 2204
BbvI GCAGC 7 cut(s) 153, 451, 553, 602, 1090, 1231, 1572
BccI CCATC 7 cut(s) 89, 515, 750, 1466, 1886, 1921, 2080
BceAI ACGGC 4 cut(s) 60, 406, 800, 986
BcgI CGANNNNNNTGC 4 cut(s) 638, 672, 1246, 1280
BciT130I CCWGG 2 cut(s) 628, 2312
BciVI GTATCC 1 cut(s) 607
BclI TGATCA 3 cut(s) 547, 666, 694
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 5 cut(s) 200, 718, 1482, 2223, 2461
BcuI ACTAGT 1 cut(s) 1997
BfaI CTAG 3 cut(s) 1566, 1913, 1998
BfmI CTRYAG 4 cut(s) 1542, 2232, 2443, 2521
BfoI RGCGCY 5 cut(s) 86, 106, 1220, 1566, 2403
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 8 cut(s) 142, 440, 564, 567, 591, 1104, 1245, 1561
BlsI GCNGC 8 cut(s) 143, 441, 565, 568, 592, 1105, 1246, 1562
Bme1390I CCNGG 3 cut(s) 393, 628, 2312
Bme18I GGWCC 9 cut(s) 163, 332, 503, 702, 738, 902, 1058, 1700, 2030
BmgBI CACGTC 1 cut(s) 199
BmiI GGNNCC 8 cut(s) 124, 505, 562, 624, 704, 904, 2031, 2434
BmrFI CCNGG 3 cut(s) 393, 628, 2312
BmrI ACTGGG 1 cut(s) 1797
BmsI GCATC 9 cut(s) 500, 668, 922, 1126, 1200, 1276, 1348, 1616, 2525
BmtI GCTAGC 1 cut(s) 1569
BmuI ACTGGG 1 cut(s) 1797
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 4 cut(s) 208, 296, 807, 1794
BpmI CTGGAG 1 cut(s) 1339
Bpu10I CCTNAGC 1 cut(s) 1312
Bpu14I TTCGAA 1 cut(s) 325
BpuEI CTTGAG 2 cut(s) 2312, 2337
BpuMI CCSGG 1 cut(s) 393
BsaAI YACGTR 1 cut(s) 2092
BsaHI GRCGYC 1 cut(s) 47
BsaI GGTCTC 3 cut(s) 200, 718, 1482
BsaJI CCNNGG 5 cut(s) 626, 627, 868, 1575, 2377
BsaXI ACNNNNNCTCC 2 cut(s) 883, 913
Bsc4I CCNNNNNNNGG 9 cut(s) 112, 242, 735, 747, 974, 1863, 2000, 2140, 2486
Bse1I ACTGG 7 cut(s) 236, 247, 794, 1337, 1702, 1803, 1979
Bse3DI GCAATG 2 cut(s) 685, 1029
BseBI CCWGG 2 cut(s) 628, 2312
BseDI CCNNGG 5 cut(s) 626, 627, 868, 1575, 2377
BseGI GGATG 4 cut(s) 761, 1363, 1932, 2516
BseLI CCNNNNNNNGG 9 cut(s) 112, 242, 735, 747, 974, 1863, 2000, 2140, 2486
BseMI GCAATG 2 cut(s) 685, 1029
BseMII CTCAG 8 cut(s) 141, 509, 1287, 1326, 1625, 1766, 1832, 2090
BseNI ACTGG 7 cut(s) 236, 247, 794, 1337, 1702, 1803, 1979
BseSI GKGCMC 2 cut(s) 321, 2300
BseXI GCAGC 7 cut(s) 153, 451, 553, 602, 1090, 1231, 1572
BsiHKAI GWGCWC 3 cut(s) 30, 321, 2204
BsiSI CCGG 2 cut(s) 161, 393
BslFI GGGAC 3 cut(s) 889, 2043, 2371
BslI CCNNNNNNNGG 9 cut(s) 112, 242, 735, 747, 974, 1863, 2000, 2140, 2486
BsmAI GTCTC 5 cut(s) 200, 718, 1482, 2223, 2461
BsmFI GGGAC 3 cut(s) 889, 2043, 2371
Bso31I GGTCTC 3 cut(s) 200, 718, 1482
Bsp119I TTCGAA 1 cut(s) 325
Bsp1286I GDGCHC 5 cut(s) 30, 321, 1313, 2204, 2300
Bsp1407I TGTACA 2 cut(s) 1705, 2413
Bsp19I CCATGG 2 cut(s) 868, 2377
BspACI CCGC 6 cut(s) 442, 564, 873, 889, 1756, 1969
BspCNI CTCAG 8 cut(s) 142, 508, 1286, 1325, 1626, 1765, 1831, 2089
BspLI GGNNCC 8 cut(s) 124, 505, 562, 624, 704, 904, 2031, 2434
BspMAI CTGCAG 1 cut(s) 1546
BspOI GCTAGC 1 cut(s) 1569
BspPI GGATC 5 cut(s) 1099, 1585, 2150, 2427, 2440
BspT104I TTCGAA 1 cut(s) 325
BspTNI GGTCTC 3 cut(s) 200, 718, 1482
BsrDI GCAATG 2 cut(s) 685, 1029
BsrGI TGTACA 2 cut(s) 1705, 2413
BsrI ACTGG 7 cut(s) 236, 247, 794, 1337, 1702, 1803, 1979
BssECI CCNNGG 5 cut(s) 626, 627, 868, 1575, 2377
BssNI GRCGYC 1 cut(s) 47
BssT1I CCWWGG 2 cut(s) 868, 2377
Bst2UI CCWGG 2 cut(s) 628, 2312
Bst4CI ACNGT 8 cut(s) 64, 295, 1078, 1144, 1486, 1579, 1645, 2063
BstACI GRCGYC 1 cut(s) 47
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstAUI TGTACA 2 cut(s) 1705, 2413
BstBAI YACGTR 1 cut(s) 2092
BstBI TTCGAA 1 cut(s) 325
BstC8I GCNNGC 8 cut(s) 307, 595, 645, 823, 1176, 1542, 1567, 2069
BstDSI CCRYGG 3 cut(s) 868, 1575, 2377
BstEII GGTNACC 2 cut(s) 977, 2474
BstENI CCTNNNNNAGG 1 cut(s) 2138
BstF5I GGATG 4 cut(s) 761, 1363, 1932, 2516
BstH2I RGCGCY 5 cut(s) 86, 106, 1220, 1566, 2403
BstHHI GCGC 7 cut(s) 59, 85, 105, 347, 1219, 1565, 2402
BstMAI GTCTC 5 cut(s) 200, 718, 1482, 2223, 2461
BstMWI GCNNNNNNNGC 8 cut(s) 560, 599, 1028, 1103, 1520, 1566, 1975, 2513
BstNI CCWGG 2 cut(s) 628, 2312
BstNSI RCATGY 2 cut(s) 1101, 2210
BstPAI GACNNNNGTC 1 cut(s) 212
BstPI GGTNACC 2 cut(s) 977, 2474
BstSCI CCNGG 3 cut(s) 391, 626, 2310
BstSFI CTRYAG 4 cut(s) 1542, 2232, 2443, 2521
BstSLI GKGCMC 2 cut(s) 321, 2300
BstV1I GCAGC 7 cut(s) 153, 451, 553, 602, 1090, 1231, 1572
BstV2I GAAGAC 4 cut(s) 208, 296, 807, 1794
BstX2I RGATCY 2 cut(s) 1590, 2432
BstYI RGATCY 2 cut(s) 1590, 2432
BsuI GTATCC 1 cut(s) 607
BtgI CCRYGG 3 cut(s) 868, 1575, 2377
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 4 cut(s) 761, 1363, 1932, 2516
BtsI GCAGTG 1 cut(s) 1224
BtsIMutI CAGTG 8 cut(s) 801, 1032, 1140, 1224, 1281, 1695, 1810, 1826
Cac8I GCNNGC 8 cut(s) 307, 595, 645, 823, 1176, 1542, 1567, 2069
CaiI CAGNNNCTG 2 cut(s) 305, 1688
CfoI GCGC 7 cut(s) 59, 85, 105, 347, 1219, 1565, 2402
CseI GACGC 3 cut(s) 36, 659, 2437
CsiI ACCWGGT 1 cut(s) 2310
Csp6I GTAC 7 cut(s) 865, 1426, 1706, 1867, 1972, 2086, 2414
CspCI CAANNNNNGTGG 2 cut(s) 953, 988
CviQI GTAC 7 cut(s) 865, 1426, 1706, 1867, 1972, 2086, 2414
DraIII CACNNNGTG 1 cut(s) 1276
EaeI YGGCCR 5 cut(s) 158, 1029, 1507, 1976, 2380
EciI GGCGGA 1 cut(s) 904
Eco130I CCWWGG 2 cut(s) 868, 2377
Eco24I GRGCYC 1 cut(s) 1313
Eco31I GGTCTC 3 cut(s) 200, 718, 1482
Eco32I GATATC 1 cut(s) 2169
Eco47I GGWCC 9 cut(s) 163, 332, 503, 702, 738, 902, 1058, 1700, 2030
Eco47III AGCGCT 3 cut(s) 1218, 1564, 2401
Eco57I CTGAAG 2 cut(s) 1320, 1637
Eco72I CACGTG 1 cut(s) 2092
Eco91I GGTNACC 2 cut(s) 977, 2474
EcoNI CCTNNNNNAGG 1 cut(s) 2138
EcoO109I RGGNCCY 3 cut(s) 503, 2030, 2276
EcoO65I GGTNACC 2 cut(s) 977, 2474
EcoRI GAATTC 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 626, 2310
EcoRV GATATC 1 cut(s) 2169
EcoT14I CCWWGG 2 cut(s) 868, 2377
EcoT22I ATGCAT 3 cut(s) 493, 1269, 1363
EcoT38I GRGCYC 1 cut(s) 1313
ErhI CCWWGG 2 cut(s) 868, 2377
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FaqI GGGAC 3 cut(s) 889, 2043, 2371
FauI CCCGC 1 cut(s) 866
FauNDI CATATG 2 cut(s) 337, 556
FbaI TGATCA 3 cut(s) 547, 666, 694
Fnu4HI GCNGC 8 cut(s) 142, 440, 564, 567, 591, 1104, 1245, 1561
FokI GGATG 4 cut(s) 768, 1370, 1939, 2503
FriOI GRGCYC 1 cut(s) 1313
Fsp4HI GCNGC 8 cut(s) 142, 440, 564, 567, 591, 1104, 1245, 1561
FspBI CTAG 3 cut(s) 1566, 1913, 1998
FspI TGCGCA 1 cut(s) 346
GlaI GCGC 7 cut(s) 58, 84, 104, 346, 1218, 1564, 2401
GluI GCNGC 8 cut(s) 142, 440, 564, 567, 591, 1104, 1245, 1561
GsuI CTGGAG 1 cut(s) 1339
HaeII RGCGCY 5 cut(s) 86, 106, 1220, 1566, 2403
HapII CCGG 2 cut(s) 161, 393
HgaI GACGC 3 cut(s) 36, 659, 2437
HhaI GCGC 7 cut(s) 59, 85, 105, 347, 1219, 1565, 2402
Hin1I GRCGYC 1 cut(s) 47
Hin6I GCGC 7 cut(s) 57, 83, 103, 345, 1217, 1563, 2400
HinP1I GCGC 7 cut(s) 57, 83, 103, 345, 1217, 1563, 2400
HindIII AAGCTT 2 cut(s) 482, 1007
HinfI GANTC 6 cut(s) 1252, 1292, 1499, 2012, 2216, 2252
HpaII CCGG 2 cut(s) 161, 393
HphI GGTGA 8 cut(s) 100, 971, 1949, 1960, 2165, 2213, 2220, 2486
Hpy166II GTNNAC 7 cut(s) 319, 447, 738, 902, 1081, 1761, 1951
Hpy188III TCNNGA 7 cut(s) 149, 205, 220, 716, 1469, 1889, 2078
Hpy8I GTNNAC 7 cut(s) 319, 447, 738, 902, 1081, 1761, 1951
Hpy99I CGWCG 4 cut(s) 46, 52, 653, 2453
HpyAV CCTTC 8 cut(s) 332, 469, 1348, 1475, 1526, 1661, 2259, 2413
HpyCH4III ACNGT 8 cut(s) 64, 295, 1078, 1144, 1486, 1579, 1645, 2063
HpyCH4IV ACGT 6 cut(s) 198, 213, 1065, 1148, 1460, 2091
HpyF10VI GCNNNNNNNGC 8 cut(s) 560, 599, 1028, 1103, 1520, 1566, 1975, 2513
HpySE526I ACGT 6 cut(s) 198, 213, 1065, 1148, 1460, 2091
Hsp92I GRCGYC 1 cut(s) 47
HspAI GCGC 7 cut(s) 57, 83, 103, 345, 1217, 1563, 2400
Ksp22I TGATCA 3 cut(s) 547, 666, 694
LmnI GCTCC 7 cut(s) 128, 658, 940, 1384, 1664, 1891, 2367
Lsp1109I GCAGC 7 cut(s) 153, 451, 553, 602, 1090, 1231, 1572
LweI GCATC 9 cut(s) 500, 668, 922, 1126, 1200, 1276, 1348, 1616, 2525
MabI ACCWGGT 1 cut(s) 2310
MaeI CTAG 3 cut(s) 1566, 1913, 1998
MaeII ACGT 6 cut(s) 198, 213, 1065, 1148, 1460, 2091
MfeI CAATTG 3 cut(s) 401, 939, 1623
MflI RGATCY 2 cut(s) 1590, 2432
MhlI GDGCHC 5 cut(s) 30, 321, 1313, 2204, 2300
MlsI TGGCCA 3 cut(s) 1031, 1509, 2382
MluNI TGGCCA 3 cut(s) 1031, 1509, 2382
MlyI GAGTC 1 cut(s) 2225
MmeI TCCRAC 7 cut(s) 1304, 1354, 1362, 2018, 2067, 2183, 2410
Mox20I TGGCCA 3 cut(s) 1031, 1509, 2382
Mph1103I ATGCAT 3 cut(s) 493, 1269, 1363
MroXI GAANNNNTTC 2 cut(s) 237, 1813
MscI TGGCCA 3 cut(s) 1031, 1509, 2382
MseI TTAA 8 cut(s) 279, 582, 1221, 1236, 1433, 1530, 1881, 2463
MslI CAYNNNNRTG 3 cut(s) 340, 602, 2293
Msp20I TGGCCA 3 cut(s) 1031, 1509, 2382
MspA1I CMGCKG 5 cut(s) 442, 566, 1403, 1711, 1756
MspI CCGG 2 cut(s) 161, 393
MspR9I CCNGG 3 cut(s) 393, 628, 2312
MunI CAATTG 3 cut(s) 401, 939, 1623
MvaI CCWGG 2 cut(s) 628, 2312
MwoI GCNNNNNNNGC 8 cut(s) 560, 599, 1028, 1103, 1520, 1566, 1975, 2513
NciI CCSGG 1 cut(s) 393
NcoI CCATGG 2 cut(s) 868, 2377
NdeI CATATG 2 cut(s) 337, 556
NheI GCTAGC 1 cut(s) 1565
NlaIV GGNNCC 8 cut(s) 124, 505, 562, 624, 704, 904, 2031, 2434
NmeAIII GCCGAG 1 cut(s) 131
NmuCI GTSAC 8 cut(s) 64, 977, 1144, 1412, 1444, 2117, 2226, 2474
NsbI TGCGCA 1 cut(s) 346
NsiI ATGCAT 3 cut(s) 493, 1269, 1363
NspI RCATGY 2 cut(s) 1101, 2210
NspV TTCGAA 1 cut(s) 325
OliI CACNNNNGTG 1 cut(s) 2293
PasI CCCWGGG 1 cut(s) 627
PciI ACATGT 1 cut(s) 2206
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 813
PdmI GAANNNNTTC 2 cut(s) 237, 1813
PfeI GAWTC 5 cut(s) 1252, 1292, 1499, 2012, 2252
PflFI GACNNNGTC 1 cut(s) 46
PflMI CCANNNNNTGG 3 cut(s) 735, 2000, 2486
PkrI GCNGC 8 cut(s) 143, 441, 565, 568, 592, 1105, 1246, 1562
PleI GAGTC 1 cut(s) 2224
PmaCI CACGTG 1 cut(s) 2092
PmlI CACGTG 1 cut(s) 2092
PpsI GAGTC 1 cut(s) 2224
Ppu21I YACGTR 1 cut(s) 2092
PpuMI RGGWCCY 2 cut(s) 503, 2030
PscI ACATGT 1 cut(s) 2206
PshAI GACNNNNGTC 1 cut(s) 212
PshBI ATTAAT 1 cut(s) 1530
PsiI TTATAA 1 cut(s) 2037
Psp5II RGGWCCY 2 cut(s) 503, 2030
Psp6I CCWGG 2 cut(s) 626, 2310
PspCI CACGTG 1 cut(s) 2092
PspEI GGTNACC 2 cut(s) 977, 2474
PspGI CCWGG 2 cut(s) 626, 2310
PspN4I GGNNCC 8 cut(s) 124, 505, 562, 624, 704, 904, 2031, 2434
PspPPI RGGWCCY 2 cut(s) 503, 2030
PstI CTGCAG 1 cut(s) 1546
PstNI CAGNNNCTG 2 cut(s) 305, 1688
PsuI RGATCY 2 cut(s) 1590, 2432
PsyI GACNNNGTC 1 cut(s) 46
PvuII CAGCTG 2 cut(s) 1403, 1711
RsaI GTAC 7 cut(s) 866, 1427, 1707, 1868, 1973, 2087, 2415
RsaNI GTAC 7 cut(s) 865, 1426, 1706, 1867, 1972, 2086, 2414
RseI CAYNNNNRTG 3 cut(s) 340, 602, 2293
SaqAI TTAA 8 cut(s) 279, 582, 1221, 1236, 1433, 1530, 1881, 2463
SatI GCNGC 8 cut(s) 142, 440, 564, 567, 591, 1104, 1245, 1561
SbfI CCTGCAGG 1 cut(s) 1546
SchI GAGTC 1 cut(s) 2225
ScrFI CCNGG 3 cut(s) 393, 628, 2312
SdaI CCTGCAGG 1 cut(s) 1546
SduI GDGCHC 5 cut(s) 30, 321, 1313, 2204, 2300
SexAI ACCWGGT 1 cut(s) 2310
SfaNI GCATC 9 cut(s) 500, 668, 922, 1126, 1200, 1276, 1348, 1616, 2525
SfcI CTRYAG 4 cut(s) 1542, 2232, 2443, 2521
SfuI TTCGAA 1 cut(s) 325
SinI GGWCC 9 cut(s) 163, 332, 503, 702, 738, 902, 1058, 1700, 2030
SmiMI CAYNNNNRTG 3 cut(s) 340, 602, 2293
SmlI CTYRAG 2 cut(s) 2291, 2352
SmoI CTYRAG 2 cut(s) 2291, 2352
SpeI ACTAGT 1 cut(s) 1997
Sse8387I CCTGCAGG 1 cut(s) 1546
SsiI CCGC 6 cut(s) 442, 564, 873, 889, 1756, 1969
SspMI CTAG 3 cut(s) 1566, 1913, 1998
StyD4I CCNGG 3 cut(s) 391, 626, 2310
StyI CCWWGG 2 cut(s) 868, 2377
TaaI ACNGT 8 cut(s) 64, 295, 1078, 1144, 1486, 1579, 1645, 2063
TaiI ACGT 6 cut(s) 201, 216, 1068, 1151, 1463, 2094
TaqI TCGA 4 cut(s) 179, 204, 325, 2451
TaqII GACCGA 1 cut(s) 755
TatI WGTACW 2 cut(s) 1705, 2413
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 5 cut(s) 1252, 1292, 1499, 2012, 2252
Tru1I TTAA 8 cut(s) 279, 582, 1221, 1236, 1433, 1530, 1881, 2463
Tru9I TTAA 8 cut(s) 279, 582, 1221, 1236, 1433, 1530, 1881, 2463
TscAI CASTG 8 cut(s) 801, 1039, 1147, 1231, 1281, 1702, 1810, 1826
TseFI GTSAC 8 cut(s) 64, 977, 1144, 1412, 1444, 2117, 2226, 2474
TseI GCWGC 7 cut(s) 141, 439, 566, 590, 1103, 1244, 1560
Tsp45I GTSAC 8 cut(s) 64, 977, 1144, 1412, 1444, 2117, 2226, 2474
TspDTI ATGAA 7 cut(s) 376, 485, 1252, 1347, 1481, 1508, 2091
TspGWI ACGGA 4 cut(s) 201, 425, 1968, 2358
TspRI CASTG 8 cut(s) 801, 1039, 1147, 1231, 1281, 1702, 1810, 1826
Tth111I GACNNNGTC 1 cut(s) 46
Van91I CCANNNNNTGG 3 cut(s) 735, 2000, 2486
VneI GTGCAC 1 cut(s) 317
VpaK11BI GGWCC 9 cut(s) 163, 332, 503, 702, 738, 902, 1058, 1700, 2030
VspI ATTAAT 1 cut(s) 1530
XagI CCTNNNNNAGG 1 cut(s) 2138
XapI RAATTY 5 cut(s) 281, 396, 1045, 1159, 2330
XceI RCATGY 2 cut(s) 1101, 2210
XcmI CCANNNNNNNNNTGG 2 cut(s) 254, 1582
XmnI GAANNNNTTC 2 cut(s) 237, 1813
XspI CTAG 3 cut(s) 1566, 1913, 1998
Zsp2I ATGCAT 3 cut(s) 493, 1269, 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.