RLG00000035321

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006465
Physical Location & Seq
Reverse (-)
64945768 .. 64951518
5751 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035321

Sequence Viewer

Length: 2526 bp
ATGGCAGTGGCAATGACAGGACTTGAGTTTAAGATAGTGGTGCTTTTGGTGGTCGGTGTTTTGGCGACGGCGTCGTATTGTGCAACTGTTAGTTACGACCACAGGGCGCTTGTGATTGATGGAAAGCGCAGAGTGTTGGTCTCTGGCTCTATTCACTACCCTCGTAGCACTCCAGAGATGTGGCCGGACCTTATTCAGAAATCCAAGGATGGAGGACTGGATGTGATCGAGACCTATGTGTTCTGGAACTCACATGAACCGGTTCGAGGCAAGTACAATTTTGAGGGAAGGAACGATTTGGTTGGATTTGTGAAGGCAGTGGCAGCAGCAGGGCTCTATGTGCATCTTCGAATTGGGCCTTATGTTTGCGCAGAATGGAATTATGGTGGCTTTCCTCTCTGGCTGCATTTTATACCCGGAATTAAGTTGCGGACTGATAATGAGCCGTACAAGGCAGAAATGCAGCGGTTCACGGCCAAAATAGTTGAAATGATGAAGAGTGAGAAGCTTTATGCATCTCAGGGAGGACCCATAATTTTATCCCAGATCGAGAACGAGTATGGAAACATTGATAAACCATATGGGCCTGCCGCCAAAACCTACATTAACTGGGCAGCAAACATGGCTGTGTCTTTGGATACAGGGGTGCCCTGGGTCATGTGCCAGCAAGCAGATGCTCCTCCTTCAGTGATCAACACTTGCAATGGATTTTACTGTGATCAATTCACCCCAAATTCTAACAGCACACCCAAAATTTGGACCGAGAATTGGAGTGGATGGTTTCTTTCATTTGGCGGTGCTGTACCTCAGAGACCGGTGGAAGACCTTGCATTTTCTGTAGCACGCTTCTACCAGCGAGGTGGAACTTTCCAAAATTATTATATGTACCATGGTGGAACCAACTTTGGGCGGTCCAGTGGAGGACCTTTTATTGCTACAAGTTATGACTATGATGCTCCACTCGATGAGTATGGACTTCTTAGGCAGCCAAAGTGGGGTCATCTAAAAGATGTGCATAAGGCGATAAAGCTTTGTGAAGCTGCAATGGTGGCCACTGACCCAACAATTTCTTCTCTTGGTCAAAACATTGAGGCAGCTGTTTACAAAACAGGATCAGTATGTTCTGCTTTTCTTGCCAATGTCGACACCAAATCTGATGCCACTGTGACTTTCAATGGAAATTCATATCAATTGCCTGCATGGTCTGTGAGCATCTTACCTGACTGCAAAAATGTAGTGCTTAACACCGCAAAGATTAACACTGCCGCTATGATTCCAAGCTTCACGCGTCAATCAATTTCAGCTGATGTTGAGCCCACTGGAGCTGTGGGCTCAGGCTGGAGTTGGATAAATGAACCAGTGGGTATCTCAAAGGCTGATGCATTTACTAGAAAGGGATTATTGGAGCAGATAAACACTACAGCTGATAAAAGTGACTATTTATGGTACTCTACAAGCATTGATGTCAAAGATGGATATAAAGCTGATCTTCATGTGGAATCGCTTGGTCATGCTCTTCATGCTTTTGTCAATGGAAAGCTTGCAGGGAGTGGAACAGGCAACAGTGGCAATGCCAAGGTTTCTGTGGACATCCCTGTCGAATTTGCATCTGGGAAGAACAAAATTGATCTTCTGAGTCTGACTGTTGGGCTTCAGAATTATGGATCTTTCTTTGACTTGGAGGGTGCTGGTATCACTGGTCCTGTACAACTGAAAGGCTCAACAAGTGGCACTACTATTGATCTTTCTTCTCAACAATGGACATATCAGATTGGACTTAAAGGTGAAGATGAAGATCTTCCCAGTGGAAGTTCTCAGTGGATTTCACAACCTACCTTGCCCAAGAATCAGCCATTGACGTGGTACAAGACCCAATTTGATGCCCCTGGTGGCAGTAACCCTGTTGCACTAGACTTCACTGGAATGGGGAAGGGTGAGGCATGGGTGAATGGACAAAGCATTGGACGTTACTGGCCCACTAATATTGCTCCAAAAAGTGGTTGCCCTGACACTTGCAATTACAGAGGAGCTTATAGTGCAGACAAATGCCGAAAGAACTGTGGCAAGCCATCTCAAGAATTGTACCACGTTCCTCGTTCATGGCTGAAATCCAGTGGCAATACTCTTGTATTATTTGAAGAAGTTGGGGGTGATCCGACACAGTTATCTTTTGCCACGAGACAGGTAGAAAGTTTGTGCTCTCATGTATCAGAGTCTCACCCATCACCTGTAGATATGTGGAGTTCAGATTCAAAAGCAGGAAGGAAGTCTCGGCCGAGGCTATCACTTGAGTGCCCATTTCCTAATCAAGTCATTTCTTCAATCAAGTTTGCAAGTTACGGAAGACCTAGTGGGACTTGTGGGAGCTTTAGCCATGGCTCGTGCAGGAGCCGTAGCGCTCTATCCATTGTACAGAAGGCGTGCATTGGATCAAAGAGTTGTAGCATCGAAGTCTCGACTCATACATTTGGTGACCCATGTAAAGGAGTAGCAAAGAGTTTAGCAGTAGAAGTTTCTTGTAAATAG

Protein Analysis

842

Amino Acids

91.33

Weight (kDa)

7.47

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 341 5.2e-122 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 58 - 205 3.2e-06 Beta-galactosidase
GHD PF17834 365 - 419 1.1e-23 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 619 - 707 9.4e-26 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 763 - 840 6.7e-22 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1595
Acc16I TGCGCA 1 cut(s) 370
AccB1I GGYRCC 1 cut(s) 646
AccB7I CCANNNNNTGG 2 cut(s) 756, 1997
AccI GTMKAC 1 cut(s) 1143
AccII CGCG 1 cut(s) 1288
AciI CCGC 7 cut(s) 430, 466, 591, 795, 910, 1248, 1266
AclWI GGATC 4 cut(s) 1120, 1672, 2147, 2437
AcoI YGGCCR 4 cut(s) 182, 474, 1050, 2273
AcsI RAATTY 4 cut(s) 733, 753, 1180, 1601
AcuI CTGAAG 2 cut(s) 669, 1637
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 9 cut(s) 275, 449, 804, 887, 1448, 1707, 1865, 2084, 2412
AfeI AGCGCT 1 cut(s) 2398
AfiI CCNNNNNNNGG 7 cut(s) 266, 756, 768, 906, 995, 1997, 2483
AflIII ACRYGT 1 cut(s) 1286
AgeI ACCGGT 2 cut(s) 259, 814
AgsI TTSAA 5 cut(s) 488, 1174, 2138, 2253, 2322
AjiI CACGTC 1 cut(s) 1860
AjnI CCWGG 2 cut(s) 650, 1885
AjuI GAANNNNNNNTTGG 2 cut(s) 1054, 1086
AleI CACNNNNGTG 1 cut(s) 2290
Alw21I GWGCWC 1 cut(s) 2201
Alw26I GTCTC 7 cut(s) 145, 224, 805, 2173, 2220, 2274, 2458
AlwI GGATC 4 cut(s) 1120, 1672, 2147, 2437
Aor51HI AGCGCT 1 cut(s) 2398
AoxI GGCC 7 cut(s) 182, 356, 474, 584, 1050, 1973, 2273
ApeKI GCWGC 8 cut(s) 323, 326, 403, 463, 614, 985, 1040, 1094
ApoI RAATTY 4 cut(s) 733, 753, 1180, 1601
AsiGI ACCGGT 2 cut(s) 259, 814
Asp700I GAANNNNTTC 2 cut(s) 261, 1797
AspLEI GCGC 4 cut(s) 109, 129, 371, 2399
AspS9I GGNCC 9 cut(s) 187, 356, 527, 584, 759, 912, 923, 1700, 1974
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 8 cut(s) 718, 1796, 1946, 1957, 2162, 2210, 2217, 2483
AsuII TTCGAA 1 cut(s) 349
AvaII GGWCC 6 cut(s) 187, 527, 759, 912, 923, 1700
BaeGI GKGCMC 2 cut(s) 651, 2297
BaeI ACNNNNGTAYC 2 cut(s) 630, 663
BalI TGGCCA 1 cut(s) 1052
BanI GGYRCC 1 cut(s) 646
BanII GRGCYC 3 cut(s) 336, 1317, 1334
BauI CACGAG 2 cut(s) 2176, 2380
BbsI GAAGAC 2 cut(s) 828, 2350
Bbv12I GWGCWC 1 cut(s) 2201
BbvI GCAGC 8 cut(s) 335, 338, 390, 475, 626, 997, 1027, 1106
BccI CCATC 6 cut(s) 113, 203, 771, 1466, 2077, 2230
BceAI ACGGC 4 cut(s) 84, 430, 489, 2376
BciT130I CCWGG 2 cut(s) 652, 1887
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 2 cut(s) 690, 718
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 7 cut(s) 145, 224, 805, 2173, 2220, 2274, 2458
BfaI CTAG 3 cut(s) 1389, 1910, 2349
BfmI CTRYAG 3 cut(s) 837, 1419, 2229
BfoI RGCGCY 2 cut(s) 110, 2400
BfuI GTATCC 1 cut(s) 631
BglII AGATCT 1 cut(s) 1795
Bme1390I CCNGG 3 cut(s) 417, 652, 1887
Bme18I GGWCC 6 cut(s) 187, 527, 759, 912, 923, 1700
BmgBI CACGTC 1 cut(s) 1860
BmgT120I GGNCC 9 cut(s) 187, 356, 527, 584, 759, 912, 923, 1700, 1974
BmiI GGNNCC 4 cut(s) 529, 648, 898, 2390
BmrFI CCNGG 3 cut(s) 417, 652, 1887
BmrI ACTGGG 2 cut(s) 619, 1797
BmuI ACTGGG 2 cut(s) 619, 1797
BpiI GAAGAC 2 cut(s) 828, 2350
BpmI CTGGAG 3 cut(s) 156, 1341, 1360
Bpu10I CCTNAGC 1 cut(s) 1333
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 3 cut(s) 44, 2058, 2309
BpuMI CCSGG 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 1794
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 3 cut(s) 145, 224, 805
BsaJI CCNNGG 8 cut(s) 204, 650, 651, 889, 1575, 1885, 2276, 2374
BsaWI WCCGGW 2 cut(s) 259, 814
Bsc4I CCNNNNNNNGG 7 cut(s) 266, 756, 768, 906, 995, 1997, 2483
Bse118I RCCGGY 2 cut(s) 259, 814
Bse3DI GCAATG 4 cut(s) 18, 709, 1050, 1576
Bse8I GATNNNNATC 1 cut(s) 1794
BseBI CCWGG 2 cut(s) 652, 1887
BseDI CCNNGG 8 cut(s) 204, 650, 651, 889, 1575, 1885, 2276, 2374
BseGI GGATG 4 cut(s) 214, 226, 782, 1590
BseJI GATNNNNATC 1 cut(s) 1794
BseLI CCNNNNNNNGG 7 cut(s) 266, 756, 768, 906, 995, 1997, 2483
BseMI GCAATG 4 cut(s) 18, 709, 1050, 1576
BseMII CTCAG 5 cut(s) 533, 821, 1347, 1625, 1829
BseRI GAGGAG 2 cut(s) 669, 2040
BseSI GKGCMC 2 cut(s) 651, 2297
BseX3I CGGCCG 1 cut(s) 2273
BseXI GCAGC 8 cut(s) 335, 338, 390, 475, 626, 997, 1027, 1106
BsgI GTGCAG 2 cut(s) 2058, 2404
Bsh1236I CGCG 1 cut(s) 1288
Bsh1285I CGRYCG 1 cut(s) 2276
BshFI GGCC 7 cut(s) 184, 358, 476, 586, 1052, 1975, 2275
BshNI GGYRCC 1 cut(s) 646
BshTI ACCGGT 2 cut(s) 259, 814
BsiEI CGRYCG 1 cut(s) 2276
BsiHKAI GWGCWC 1 cut(s) 2201
BsiSI CCGG 4 cut(s) 185, 260, 417, 815
BslFI GGGAC 1 cut(s) 2368
BslI CCNNNNNNNGG 7 cut(s) 266, 756, 768, 906, 995, 1997, 2483
BsmAI GTCTC 7 cut(s) 145, 224, 805, 2173, 2220, 2274, 2458
BsmFI GGGAC 1 cut(s) 2368
BsnI GGCC 7 cut(s) 184, 358, 476, 586, 1052, 1975, 2275
Bso31I GGTCTC 3 cut(s) 145, 224, 805
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 6 cut(s) 336, 651, 1317, 1334, 2201, 2297
Bsp1407I TGTACA 2 cut(s) 1705, 2410
Bsp19I CCATGG 2 cut(s) 889, 2374
BspACI CCGC 7 cut(s) 430, 466, 591, 795, 910, 1248, 1266
BspANI GGCC 7 cut(s) 184, 358, 476, 586, 1052, 1975, 2275
BspCNI CTCAG 5 cut(s) 532, 820, 1346, 1626, 1828
BspFNI CGCG 1 cut(s) 1288
BspLI GGNNCC 4 cut(s) 529, 648, 898, 2390
BspPI GGATC 4 cut(s) 1120, 1672, 2147, 2437
BspQI GCTCTTC 1 cut(s) 1521
BspT104I TTCGAA 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 646
BspTNI GGTCTC 3 cut(s) 145, 224, 805
BsrDI GCAATG 4 cut(s) 18, 709, 1050, 1576
BsrFI RCCGGY 2 cut(s) 259, 814
BsrGI TGTACA 2 cut(s) 1705, 2410
BssAI RCCGGY 2 cut(s) 259, 814
BssECI CCNNGG 8 cut(s) 204, 650, 651, 889, 1575, 1885, 2276, 2374
BssNI GRCGYC 1 cut(s) 71
BssSI CACGAG 2 cut(s) 2176, 2380
BssT1I CCWWGG 4 cut(s) 204, 889, 1575, 2374
Bst2BI CACGAG 2 cut(s) 2176, 2380
Bst2UI CCWGG 2 cut(s) 652, 1887
Bst4CI ACNGT 7 cut(s) 88, 716, 1165, 1565, 1645, 2060, 2163
Bst6I CTCTTC 2 cut(s) 491, 1521
BstACI GRCGYC 1 cut(s) 71
BstAUI TGTACA 2 cut(s) 1705, 2410
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 8 cut(s) 588, 665, 669, 844, 1197, 1542, 2066, 2422
BstDEI CTNAG 6 cut(s) 519, 807, 980, 1333, 1634, 1815
BstDSI CCRYGG 2 cut(s) 889, 2374
BstEII GGTNACC 1 cut(s) 2471
BstF5I GGATG 4 cut(s) 214, 226, 782, 1590
BstFNI CGCG 1 cut(s) 1288
BstH2I RGCGCY 2 cut(s) 110, 2400
BstHHI GCGC 4 cut(s) 109, 129, 371, 2399
BstMAI GTCTC 7 cut(s) 145, 224, 805, 2173, 2220, 2274, 2458
BstMCI CGRYCG 1 cut(s) 2276
BstMWI GCNNNNNNNGC 8 cut(s) 323, 340, 623, 1049, 1133, 1520, 1566, 2036
BstNI CCWGG 2 cut(s) 652, 1887
BstPI GGTNACC 1 cut(s) 2471
BstSCI CCNGG 3 cut(s) 415, 650, 1885
BstSFI CTRYAG 3 cut(s) 837, 1419, 2229
BstSLI GKGCMC 2 cut(s) 651, 2297
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 8 cut(s) 335, 338, 390, 475, 626, 997, 1027, 1106
BstV2I GAAGAC 2 cut(s) 828, 2350
BstX2I RGATCY 2 cut(s) 1664, 1795
BstXI CCANNNNNNTGG 3 cut(s) 180, 860, 1860
BstYI RGATCY 2 cut(s) 1664, 1795
BstZI CGGCCG 1 cut(s) 2273
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 7 cut(s) 184, 358, 476, 586, 1052, 1975, 2275
BtgI CCRYGG 2 cut(s) 889, 2374
BtrI CACGTC 1 cut(s) 1860
BtsCI GGATG 4 cut(s) 214, 226, 782, 1590
BtsI GCAGTG 3 cut(s) 12, 324, 1260
Cac8I GCNNGC 8 cut(s) 588, 665, 669, 844, 1197, 1542, 2066, 2422
CfoI GCGC 4 cut(s) 109, 129, 371, 2399
Cfr10I RCCGGY 2 cut(s) 259, 814
Cfr13I GGNCC 9 cut(s) 187, 356, 527, 584, 759, 912, 923, 1700, 1974
CseI GACGC 2 cut(s) 60, 1277
Csp6I GTAC 9 cut(s) 274, 448, 803, 886, 1447, 1706, 1864, 2083, 2411
CspAI ACCGGT 2 cut(s) 259, 814
CspCI CAANNNNNGTGG 2 cut(s) 1966, 2001
CviQI GTAC 9 cut(s) 274, 448, 803, 886, 1447, 1706, 1864, 2083, 2411
DdeI CTNAG 6 cut(s) 519, 807, 980, 1333, 1634, 1815
DrdI GACNNNNNNGTC 1 cut(s) 1595
DseDI GACNNNNNNGTC 1 cut(s) 1595
EaeI YGGCCR 4 cut(s) 182, 474, 1050, 2273
EagI CGGCCG 1 cut(s) 2273
Eam1104I CTCTTC 2 cut(s) 491, 1521
EarI CTCTTC 2 cut(s) 491, 1521
EclXI CGGCCG 1 cut(s) 2273
Eco130I CCWWGG 4 cut(s) 204, 889, 1575, 2374
Eco24I GRGCYC 3 cut(s) 336, 1317, 1334
Eco31I GGTCTC 3 cut(s) 145, 224, 805
Eco47I GGWCC 6 cut(s) 187, 527, 759, 912, 923, 1700
Eco47III AGCGCT 1 cut(s) 2398
Eco52I CGGCCG 1 cut(s) 2273
Eco57I CTGAAG 2 cut(s) 669, 1637
Eco91I GGTNACC 1 cut(s) 2471
EcoO109I RGGNCCY 2 cut(s) 527, 923
EcoO65I GGTNACC 1 cut(s) 2471
EcoRII CCWGG 2 cut(s) 650, 1885
EcoT14I CCWWGG 4 cut(s) 204, 889, 1575, 2374
EcoT22I ATGCAT 2 cut(s) 517, 1384
EcoT38I GRGCYC 3 cut(s) 336, 1317, 1334
ErhI CCWWGG 4 cut(s) 204, 889, 1575, 2374
FalI AAGNNNNNCTT 2 cut(s) 1473, 1505
FaqI GGGAC 1 cut(s) 2368
FauNDI CATATG 1 cut(s) 580
FbaI TGATCA 2 cut(s) 690, 718
FblI GTMKAC 1 cut(s) 1143
FokI GGATG 4 cut(s) 221, 233, 789, 1577
FriOI GRGCYC 3 cut(s) 336, 1317, 1334
FspBI CTAG 3 cut(s) 1389, 1910, 2349
FspI TGCGCA 1 cut(s) 370
GlaI GCGC 4 cut(s) 108, 128, 370, 2398
GsuI CTGGAG 3 cut(s) 156, 1341, 1360
HaeII RGCGCY 2 cut(s) 110, 2400
HaeIII GGCC 7 cut(s) 184, 358, 476, 586, 1052, 1975, 2275
HapII CCGG 4 cut(s) 185, 260, 417, 815
HgaI GACGC 2 cut(s) 60, 1277
HhaI GCGC 4 cut(s) 109, 129, 371, 2399
Hin1I GRCGYC 1 cut(s) 71
Hin6I GCGC 4 cut(s) 107, 127, 369, 2397
HinP1I GCGC 4 cut(s) 107, 127, 369, 2397
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HindIII AAGCTT 4 cut(s) 506, 1028, 1279, 1538
HinfI GANTC 7 cut(s) 1273, 1499, 1636, 1846, 2213, 2249, 2458
HpaII CCGG 4 cut(s) 185, 260, 417, 815
HphI GGTGA 8 cut(s) 718, 1796, 1946, 1957, 2162, 2210, 2217, 2483
Hpy166II GTNNAC 4 cut(s) 471, 1102, 1144, 1588
Hpy188III TCNNGA 6 cut(s) 173, 229, 244, 550, 2075, 2455
Hpy8I GTNNAC 4 cut(s) 471, 1102, 1144, 1588
Hpy99I CGWCG 2 cut(s) 70, 76
HpyAV CCTTC 6 cut(s) 282, 307, 693, 1924, 2256, 2410
HpyCH4III ACNGT 7 cut(s) 88, 716, 1165, 1565, 1645, 2060, 2163
HpyCH4IV ACGT 3 cut(s) 1859, 1966, 2088
HpyF10VI GCNNNNNNNGC 8 cut(s) 323, 340, 623, 1049, 1133, 1520, 1566, 2036
HpyF3I CTNAG 6 cut(s) 519, 807, 980, 1333, 1634, 1815
HpySE526I ACGT 3 cut(s) 1859, 1966, 2088
Hsp92I GRCGYC 1 cut(s) 71
HspAI GCGC 4 cut(s) 107, 127, 369, 2397
Ksp22I TGATCA 2 cut(s) 690, 718
LguI GCTCTTC 1 cut(s) 1521
LmnI GCTCC 8 cut(s) 682, 961, 1322, 1405, 1993, 2027, 2364, 2388
Lsp1109I GCAGC 8 cut(s) 335, 338, 390, 475, 626, 997, 1027, 1106
MaeI CTAG 3 cut(s) 1389, 1910, 2349
MaeII ACGT 3 cut(s) 1859, 1966, 2088
MaeIII GTNAC 7 cut(s) 92, 1165, 1433, 1895, 1967, 2336, 2471
MfeI CAATTG 1 cut(s) 1190
MflI RGATCY 2 cut(s) 1664, 1795
MhlI GDGCHC 6 cut(s) 336, 651, 1317, 1334, 2201, 2297
MlsI TGGCCA 1 cut(s) 1052
MluI ACGCGT 1 cut(s) 1286
MluNI TGGCCA 1 cut(s) 1052
MlyI GAGTC 3 cut(s) 1645, 2222, 2452
MmeI TCCRAC 3 cut(s) 283, 1325, 2180
Mox20I TGGCCA 1 cut(s) 1052
Mph1103I ATGCAT 2 cut(s) 517, 1384
MroXI GAANNNNTTC 2 cut(s) 261, 1797
MscI TGGCCA 1 cut(s) 1052
MseI TTAA 6 cut(s) 30, 423, 606, 1242, 1257, 1779
MslI CAYNNNNRTG 4 cut(s) 626, 1858, 1922, 2290
Msp20I TGGCCA 1 cut(s) 1052
MspA1I CMGCKG 4 cut(s) 466, 1097, 1304, 1424
MspI CCGG 4 cut(s) 185, 260, 417, 815
MspR9I CCNGG 3 cut(s) 417, 652, 1887
MunI CAATTG 1 cut(s) 1190
MvaI CCWGG 2 cut(s) 652, 1887
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 8 cut(s) 323, 340, 623, 1049, 1133, 1520, 1566, 2036
NciI CCSGG 1 cut(s) 417
NcoI CCATGG 2 cut(s) 889, 2374
NdeI CATATG 1 cut(s) 580
NlaIV GGNNCC 4 cut(s) 529, 648, 898, 2390
NmeAIII GCCGAG 2 cut(s) 2251, 2301
NmuCI GTSAC 3 cut(s) 1165, 1433, 2471
NsbI TGCGCA 1 cut(s) 370
NsiI ATGCAT 2 cut(s) 517, 1384
NspV TTCGAA 1 cut(s) 349
OliI CACNNNNGTG 1 cut(s) 2290
PasI CCCWGGG 1 cut(s) 651
PciSI GCTCTTC 1 cut(s) 1521
PdmI GAANNNNTTC 2 cut(s) 261, 1797
PfeI GAWTC 4 cut(s) 1273, 1499, 1846, 2249
PflFI GACNNNGTC 1 cut(s) 70
PflMI CCANNNNNTGG 2 cut(s) 756, 1997
PinAI ACCGGT 2 cut(s) 259, 814
PleI GAGTC 3 cut(s) 1644, 2221, 2452
PpsI GAGTC 3 cut(s) 1644, 2221, 2452
PpuMI RGGWCCY 2 cut(s) 527, 923
Psp5II RGGWCCY 2 cut(s) 527, 923
Psp6I CCWGG 2 cut(s) 650, 1885
PspEI GGTNACC 1 cut(s) 2471
PspGI CCWGG 2 cut(s) 650, 1885
PspN4I GGNNCC 4 cut(s) 529, 648, 898, 2390
PspPI GGNCC 9 cut(s) 187, 356, 527, 584, 759, 912, 923, 1700, 1974
PspPPI RGGWCCY 2 cut(s) 527, 923
PsuI RGATCY 2 cut(s) 1664, 1795
PsyI GACNNNGTC 1 cut(s) 70
PvuII CAGCTG 3 cut(s) 1097, 1304, 1424
RsaI GTAC 9 cut(s) 275, 449, 804, 887, 1448, 1707, 1865, 2084, 2412
RsaNI GTAC 9 cut(s) 274, 448, 803, 886, 1447, 1706, 1864, 2083, 2411
RseI CAYNNNNRTG 4 cut(s) 626, 1858, 1922, 2290
SalI GTCGAC 1 cut(s) 1142
SapI GCTCTTC 1 cut(s) 1521
SaqAI TTAA 6 cut(s) 30, 423, 606, 1242, 1257, 1779
Sau96I GGNCC 9 cut(s) 187, 356, 527, 584, 759, 912, 923, 1700, 1974
SchI GAGTC 3 cut(s) 1645, 2222, 2452
ScrFI CCNGG 3 cut(s) 417, 652, 1887
SduI GDGCHC 6 cut(s) 336, 651, 1317, 1334, 2201, 2297
SfcI CTRYAG 3 cut(s) 837, 1419, 2229
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 6 cut(s) 187, 527, 759, 912, 923, 1700
SmiMI CAYNNNNRTG 4 cut(s) 626, 1858, 1922, 2290
SmlI CTYRAG 3 cut(s) 23, 2073, 2288
SmoI CTYRAG 3 cut(s) 23, 2073, 2288
SsiI CCGC 7 cut(s) 430, 466, 591, 795, 910, 1248, 1266
SspI AATATT 1 cut(s) 1984
SspMI CTAG 3 cut(s) 1389, 1910, 2349
StyD4I CCNGG 3 cut(s) 415, 650, 1885
StyI CCWWGG 4 cut(s) 204, 889, 1575, 2374
TaaI ACNGT 7 cut(s) 88, 716, 1165, 1565, 1645, 2060, 2163
TaiI ACGT 3 cut(s) 1862, 1969, 2091
TaqI TCGA 9 cut(s) 228, 265, 349, 549, 963, 1143, 1599, 2448, 2456
TaqII GACCGA 1 cut(s) 776
TatI WGTACW 3 cut(s) 273, 1705, 2410
TauI GCSGC 2 cut(s) 593, 1268
TfiI GAWTC 4 cut(s) 1273, 1499, 1846, 2249
Tru1I TTAA 6 cut(s) 30, 423, 606, 1242, 1257, 1779
Tru9I TTAA 6 cut(s) 30, 423, 606, 1242, 1257, 1779
TseFI GTSAC 3 cut(s) 1165, 1433, 2471
TseI GCWGC 8 cut(s) 323, 326, 403, 463, 614, 985, 1040, 1094
Tsp45I GTSAC 3 cut(s) 1165, 1433, 2471
TspDTI ATGAA 9 cut(s) 270, 509, 777, 1173, 1368, 1481, 1508, 1806, 2088
TspGWI ACGGA 1 cut(s) 2355
Tth111I GACNNNGTC 1 cut(s) 70
Van91I CCANNNNNTGG 2 cut(s) 756, 1997
VpaK11BI GGWCC 6 cut(s) 187, 527, 759, 912, 923, 1700
XapI RAATTY 4 cut(s) 733, 753, 1180, 1601
XcmI CCANNNNNNNNNTGG 2 cut(s) 1324, 1582
XmiI GTMKAC 1 cut(s) 1143
XmnI GAANNNNTTC 2 cut(s) 261, 1797
XspI CTAG 3 cut(s) 1389, 1910, 2349
Zsp2I ATGCAT 2 cut(s) 517, 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.